Search Results (6,432)

Objectives: Animal studies have demonstrated that mesenchymal stem cell-conditioned medium (MSC-CM) possesses various therapeutic effects, including anti-inflammatory and anti-fibrotic properties. This study investigated the efficacy and safety of administering MSC-CM as a treatment for patients with generalized fatigue. Methods: The MSC-CM used in this study was derived from human adipose tissue and umbilical cord-derived mesenchymal stem cells cultured in a medium free of animal-derived components to avoid the risk of infection. This MSC-CM has recently been shown to possess anti-inflammatory effects and has been reported to be safe for human administration. With the expectation of alleviating fatigue symptoms through its anti-inflammatory effects, it was administered to patients intravenously and by inhalation. Safety and changes in subjective symptoms were evaluated, and blood biomarkers related to inflammation and oxidative stress were measured. Results: In this trial involving 19 patients experiencing fatigue, no serious side effects were observed following MSC-CM administration. Nearly half of the patients reported symptom improvement after a single dose, and some exhibited signs of reduced inflammation. Conclusions: This report presents the first investigation of systemic MSC-CM treatment for generalized fatigue, paving the way for more targeted studies on dosage and treatment frequency. These findings offer new hope and possibilities for treating fatigue, providing valuable insights into the clinical application of MSC-CM. Full article

Objective: This study aimed to assess the impact of photodynamic therapy (PDT) and topical glucocorticosteroids (GKS) on total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI) in the saliva of patients with oral lichen planus (OLP). Methods: Ninety patients with histopathologically confirmed OLP were randomly assigned to either the PDT group (n = 50) or the GKS group (n = 40). Unstimulated saliva samples were collected before treatment and at 1, 3, and 6 months post-therapy. TOS, TAC, and OSI were determined using colorimetric assays. Results: Both PDT and GKS significantly reduced TOS over the entire observation period. TAC decreased persistently after GKS but remained stable after PDT except for an initial decline. OSI was significantly lower immediately after PDT but did not show sustained differences. Overall, PDT more effectively and durably restored redox balance compared to GKS. Conclusions: Photodynamic therapy demonstrates superior long-term efficacy in modulating oxidative stress markers in saliva, supporting its role as a promising alternative to topical corticosteroids in managing OLP. Clinically, these findings suggest that PDT may offer a non-invasive, recurrence-reducing, and steroid-sparing treatment alternative for OLP, potentially improving long-term patient outcomes and reducing side effects associated with prolonged corticosteroid use. Full article

Polyethylene glycol (PEG) has been widely utilized in optimizing therapeutics due to its excellent biocompatibility and chemical stability. However, multiple dosing of PEGylated drugs may result in toxicity due to PEG accumulation in tissues, leading to the formation of anti-PEG antibodies (APAs), which can accelerate drug clearance, reduce efficacy, and alongside enhanced side effects, such as allergic reactions. Notably, pre-existing APAs have also been detected in individuals with no prior exposure to PEGylated drugs, raising additional clinical concerns. This review summarizes the mechanisms of APA generation, the factors influencing PEG immunogenicity, and the biological consequences of APAs on drug pharmacokinetics, efficacy, and safety. We also discuss current challenges in APA detection and highlight strategies to minimize immunogenic responses, including PEG modification, immunomodulation, and alternative polymers. This review aims to provide a comprehensive reference for the rational design, evaluation, and clinical management of PEGylated drugs. Full article

Microgrids play a crucial role in the development of future smart grids, with multiple interconnected microgrids forming large-scale multi-microgrid systems that operate as smart grids. Multi-agent system (MAS)-based control solutions are the most suitable for addressing such control challenges. This paper presents a demand-side management (DSM) strategy using a meta-heuristic optimization technique for minimizing the household energy consumption cost using MAS. The binary grey wolf optimization algorithm (BGWOA) optimizes load scheduling, reducing electricity costs, without compromising consumer preferences using time-of-day (ToD) tariffs. The communication agents and load agents comprise the MAS used to streamline load control operations. The results demonstrate that MAS-based load control using metaheuristic optimization techniques enhances demand-side management, thus minimizing the electricity costs while adhering to contradictory parameters like user preferences, appliance duration, and load atomicity. This makes renewable energy integration more cost-effective in smart grids, thereby ensuring affordable, reliable, and sustainable energy for all. Full article

Objectives: To assess the impact of intravenous ferric carboxymaltose (FCM) on fetal and maternal adverse effects in pregnant women diagnosed with iron deficiency anemia (IDA). Methods: This is a multicenter retrospective study on 472 pregnant women diagnosed with moderate to severe IDA undergoing treatment with FCM between 2019 and 2025 at Careggi University Hospital (Florence) and Vittore Buzzi Children Hospital (Milan). Fetal wellbeing was assessed using computerized cardiotocography (cCTG) or ultrasound, based on gestational age at treatment. Maternal side effects were evaluated through clinical evaluation. Results: cCTG was performed in 377/472 patients (80%), with a mean short-term variability of 10.2 ms. Normal cCTG criteria were met in 98.4% of cases; six patients exhibited transient reduced variability, which resolved following intrauterine resuscitation. Ultrasound assessment was performed in 95 patients (20%), revealing no fetal heart rate abnormalities. Maternal side effects occurred in seven patients (1.4%). Hemoglobin concentrations increased by a median of 1.4 g/dL after five weeks, reaching up to 2.8 g/dL in women with a baseline Hb < 8 g/dL. Conclusions: Our findings support the potential safety and efficacy of intravenous FCM for the treatment of IDA during pregnancy, demonstrating low rates of maternal side effects and no adverse fetal heart rate patterns. However, prospective studies are necessary to confirm these results. Full article

Due to the high cost of hydrogen utilization and the uncertainties in renewable energy generation and load demand, significant challenges are posed for the operation optimization of hydrogen-containing integrated energy systems (IESs). In this study, a robust operational model for an electric–heat–gas IES (EHG-IES) is proposed, considering the hydrogen energy system heat recovery (HESHR) and multiple uncertainties. Firstly, a heat recovery model for the hydrogen system is established based on thermodynamic equations and reaction principles; secondly, through the constructed adjustable robust optimization (ARO) model, the optimal solution of the system under the worst-case scenario is obtained; lastly, the original problem is decomposed based on the column and constraint generation method and strong duality theory, resulting in the formulation of a master problem and subproblem with mixed-integer linear characteristics. These problems are solved through alternating iterations, ultimately obtaining the corresponding optimal scheduling scheme. The simulation results demonstrate that our model and method can effectively reduce the operation and maintenance costs of HESHR-EHG-IES while being resilient to uncertainties on both the supply and demand sides. In summary, this study provides a novel approach for the diversified utilization and flexible operation of energy in HESHR-EHG-IES, contributing to the safe, controllable, and economically efficient development of the energy market. It holds significant value for engineering practice. Full article

Background: Common bile duct (CBD) treatments are often associated with complications, limiting long-term efficacy. To overcome these issues, polymeric grafts have been suggested as promising alternatives, since they are highly customizable, biocompatible, and may reduce side effects frequency. Methods: A systematic review was conducted, interrogating MEDLINE and Cochrane Library. Next, an in vivo study involved 20 pigs, which underwent a former controlled biliary injury. To repair the defect, a α,β-Poly(N-2-hydroxyethyl)-DL-Aspartamide (PHEA)–Polylactic-acid (PLA)–Polycaprolactone (PCL) scaffold was implanted. The animals were sacrificed at one and three months for gross and histological examinations, to assess tissue integration and healing outcomes. Results: The systematic review highlighted that such scaffolds have shown promising results in CBD regeneration, both in single and joined applications. These findings were confirmed by the in vivo study, where the use of such scaffolds—particularly, the planar ones—led to safe and complete bile duct regeneration. Histological analysis revealed lymphomonocytic infiltrates and neovascularization, while microscopic examination showed progressive scaffold degradation accompanied by biliary tissue regeneration. Conclusions: Experimental results are consistent with the literature, confirming the potential of such polymeric scaffolds in aiding complete CBD regeneration and being reabsorbed shortly after. Still, further studies are needed to fully validate their translational application. PROSPERO ID: CRD420251115056. Full article

Colostrum is a nutrient-rich fluid secreted by mammals shortly after birth, primarily to provide passive immunity and support early immune development in newborns. Among its various sources, bovine colostrum is the most widely used supplement due to its high bioavailability, safety profile, and clinically supported health benefits. Rich in immunoglobulins, lactoferrin, growth factors, and antimicrobial peptides, bovine colostrum exhibits diverse biological activities that extend beyond neonatal health. Recently, the rising prevalence of cancer—driven by environmental stressors such as radiation, processed foods, and chronic inflammation, as well as non-environmental hereditary factors including germline mutations, family history, and epigenetic inheritance—has fueled interest in natural adjunctive therapies. Scientific studies have explored the anticancer potential of bovine colostrum, highlighting its ability to modulate immune responses, inhibit tumor growth, induce apoptosis in cancer cells, and reduce inflammation. Key components including lactoferrin and proline-rich peptides have been identified as contributors to these effects. Additionally, bovine colostrum may help reduce the side effects of standard cancer treatments, such as mouth sores from chemotherapy or weakened immune systems, by helping to heal tissues and boost the body’s defenses. While large-scale clinical studies are still needed, current findings suggest that bovine colostrum holds promise as a supportive element in integrative cancer care. In conclusion, bovine colostrum represents a safe, bioactive-rich natural supplement with multifaceted therapeutic potential, particularly in oncology, owing to its key components such as lactoferrin, immunoglobulins, growth factors (e.g., IGF-1, TGF-β), and proline-rich polypeptides (PRPs), which contribute to its immunomodulatory, anti-inflammatory, and potential anticancer effects. Ongoing and future research will be crucial to fully understand its mechanisms of action and establish its role in evidence-based cancer prevention and treatment strategies. Full article

There is growing evidence regarding non-pharmacological therapies such as music as a supportive approach for the treatment of various clinical conditions in humans. Physiological and neurobiological research suggests that music exposure is related to endorphin, endocannabinoid and dopamine release, favourable effects on autonomic nervous system functioning and is associated with decreased pain perception and reduced stress response. Further evidence in humans demonstrates a beneficial role of music application during the perioperative period by improving various outcome measures, such as the perioperative stress and anxiety levels, the sedation or general anaesthetic requirements, the pain levels, the analgesic requirements and other parameters related to patient prognosis, without reported side effects. Accordingly, such interventions have been considered as a method of environmental enrichment for animal welfare enhancement, by masking potentially disturbing background noises and by ameliorating anxiety or aggressive behaviours in different stressful settings in dogs and cats. Furthermore, research has been lately extended to the potential music’s effect in these species during the perioperative period, considered a stressful setting, as well. This review presents the existing evidence of music application focusing on the perioperative period of dogs and cats, as part of a multimodal approach, to improve their surgical outcome and welfare. Full article

In the context of green building development, the lighting design of campus living rooms in hot summer and cold winter areas faces the dual challenges of glare control in summer and insufficient daylight in winter. Based on BIM technology, this study uses Revit 2016 modeling and the HYBPA 2024 performance analysis platform to simulate and optimize the daylighting performance of the campus activity center of Hunan City College in multiple rounds of iterations. It is found that the traditional single large-area external window design leads to uneven lighting in 70% of the area, and the average value of the lighting coefficient is only 2.1%, which is lower than the national standard requirement of 3.3%. Through the introduction of the hybrid system of “side lighting + top light guide”, combined with adjustable inner louver shading, the optimized average value of the lighting coefficient is increased to 4.8%, the uniformity of indoor illuminance is increased from 0.35 to 0.68, the proportion of annual standard sunshine hours (≥300 lx) reaches 68.7%, and the energy consumption of the artificial lighting is reduced by 27.3%. Dynamic simulation shows that the uncomfortable glare index at noon on the summer solstice is reduced from 30.2 to 22.7, which meets the visual comfort requirements. The study confirms that the BIM-driven “static-dynamic” simulation coupling method can effectively address climate adaptability issues. However, it has limitations such as insufficient integration with international healthy building standards, insufficient accuracy of meteorological data, and simplification of indoor dynamic shading factors. Future research can focus on improving meteorological data accuracy, incorporating indoor dynamic factors, and exploring intelligent daylighting systems to deepen and expand the method, promote the integration of cross-standard evaluation systems, and provide a technical pathway for healthy lighting environment design in summer-hot and winter-cold regions. Full article

Cisplatin (CIS)-induced peripheral neuropathy and associated comorbidities have a detrimental effect on the lives of cancer patients. Currently, there are no effective therapies to alleviate these symptoms. Duloxetine (DULO) is a recommended treatment, but it is linked with important side effects, thus making it essential to explore novel approaches. We examined the impact of a prophylactic treatment with a low dose of DULO combined with hydrogen-rich water (HRW) on CIS-injected C57BL/6 male and female mice as a possible therapy for allodynia, muscle and body weight deficits, and emotive syndromes accompanying this type of chemotherapy. The prophylactic treatment with DULO and HRW prevented mechanical allodynia caused by CIS in both sexes and had greater effects than either treatment given individually. The combined treatment also prevented cold allodynia in male mice but only reduced it in females. Moreover, the coadministration of DULO with HRW avoided muscular deficits in both sexes. Furthermore, the body weight reduction induced by CIS in both sexes was not entirely mitigated by the combined therapy. However, all treatments avoided the anxiety- and depressive-like behaviors elicited by CIS. The antiallodynic actions and prevention of muscular deficits produced by the combined treatment might be explained by the inhibition of oxidative stress, inflammatory responses, and plasticity alterations provoked by CIS in the dorsal root ganglia of these subjects. This study proposes, for the first time, the cotreatment of DULO with HRW as an effective therapy for CIS-induced peripheral neuropathy and reveals the influence of sex on these actions. Full article

Current therapies for inflammatory bowel disease (IBD), such as olsalazine and cyclosporine, often exhibit limited long-term efficacy and are associated with adverse effects. Cannabidiol (CBD), a non-psychoactive phytocannabinoid, shows promise for its anti-inflammatory properties, though its effectiveness as a monotherapy remains inconclusive. This study investigates the therapeutic potential of combining low-dose CBD (10 mg/kg) with olsalazine (50 mg/kg) or cyclosporine (2.5, 5 mg/kg) in dextran sulphate sodium (DSS)-induced acute and chronic colitis models in mice. Disease severity was assessed via disease activity index (DAI), colon morphology, cytokine and chemokine expression, myeloperoxidase (MPO) activity, systemic inflammatory markers, and glucagon-like peptide-1 (GLP-1) regulation. Safety evaluations included haematology and plasma biochemistry. DSS-treated mice showed elevated DAI scores, colon shortening, heightened inflammation, and organ enlargement. Combination therapies significantly ameliorated colitis, reducing DAI, MPO activity, and inflammatory cytokines, while restoring colon length and GLP-1 levels—without inducing liver or kidney toxicity. These findings demonstrate that combining a low dose of CBD with standard IBD drugs enhances therapeutic efficacy while minimizing side effects, supporting its integration into future combination strategies for more effective and safer IBD management. Full article

Accurate real-time detection of hawthorn by vision systems is a fundamental prerequisite for automated harvesting. This study addresses the challenges in hawthorn orchards—including target overlap, leaf occlusion, and environmental variations—which lead to compromised detection accuracy, high computational resource demands, and poor real-time performance in existing methods. To overcome these limitations, we propose YOLO-DCL (group shuffling convolution and coordinate attention integrated with a lightweight head based on YOLOv8n), a novel lightweight hawthorn detection model. The backbone network employs dynamic group shuffling convolution (DGCST) for efficient and effective feature extraction. Within the neck network, coordinate attention (CA) is integrated into the feature pyramid network (FPN), forming an enhanced multi-scale feature pyramid network (HSPFN); this integration further optimizes the C2f structure. The detection head is designed utilizing shared convolution and batch normalization to streamline computation. Additionally, the PIoUv2 (powerful intersection over union version 2) loss function is introduced to significantly reduce model complexity. Experimental validation demonstrates that YOLO-DCL achieves a precision of 91.6%, recall of 90.1%, and mean average precision (mAP) of 95.6%, while simultaneously reducing the model size to 2.46 MB with only 1.2 million parameters and 4.8 GFLOPs computational cost. To rigorously assess real-world applicability, we developed and deployed a detection system based on the PySide6 framework on an NVIDIA Jetson Xavier NX edge device. Field testing validated the model’s robustness, high accuracy, and real-time performance, confirming its suitability for integration into harvesting robots operating in practical orchard environments. Full article

This review explores the therapeutic promise of natural compounds in modulating 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), a key enzyme in cholesterol synthesis. HMGCR dysregulation is implicated in dyslipidemia, cardiovascular disease, and cancer, conditions linked to oxidative stress. While statins inhibit HMGCR, their side effects necessitate exploring alternatives. The review highlights various natural compounds—flavonoids, phenolic acids, stilbenes, and herbal formulations—with HMGCR-modulating and antioxidant capabilities. In vitro and in vivo studies suggest these compounds offer a promising avenue for treating HMGCR-related conditions. Synergistic effects are observed when combining natural products with statins, hinting at combination therapies that could lower statin dosages and reduce adverse effects. Natural HMGCR modulators hold therapeutic promise but face hurdles like limited in vivo data, regulatory issues, variability in composition, potential drug interactions, and safety concerns. Future research must prioritize comprehensive mechanistic studies, standardized preparations, and well-designed clinical trials. Overcoming these challenges through rigorous science is essential for integrating natural HMGCR modulators into clinical practice and improving patient outcomes in a safe and effective manner. Specifically, clinical trials should consider combination therapies and comparison with standard treatments like statins. More research is also needed on optimal dosages and treatment regimens. Full article

Background/Objectives: Dyslipidemia is a prevalent metabolic disorder closely linked to cardiovascular complications and muscular pathologies, often managed using statins such as simvastatin. However, statin-induced myopathy remains a significant treatment-limiting side effect, necessitating the exploration of safe, natural alternatives. Spirulina platensis, a phytochemical-rich marine-derived cyanobacterium, has emerged as a promising bioactive nutraceutical with potent antioxidant and anti-inflammatory properties. This study evaluated the comparative effects of Spirulina platensis and simvastatin in attenuating dyslipidemia-induced skeletal muscle injury in adult male albino rats. Methods: Forty animals were allocated to the control and high-fat diet (HFD) groups. After 4 weeks, the dyslipidemic rats were subdivided into untreated, simvastatin-treated, and Spirulina platensis-treated subgroups. Serum lipid profile, creatine kinase (CK), and malondialdehyde (MDA) levels were assessed. Histological, ultrastructural, and immunohistochemical analyses were conducted to assess muscle fiber integrity and expression of TGF-β1 and Bcl2. Results: Spirulina platensis significantly improved lipid parameters, reduced CK and MDA levels, preserved muscle histoarchitecture, and downregulated fibrotic (↓TGF-β1) and apoptotic (↑Bcl2) responses compared to the dyslipidemic and simvastatin-treated groups. Our results proved that Spirulina platensis ameliorates the effects of statin-associated myopathy while exerting lipid-lowering, cytoprotective, and antifibrotic effects. Conclusion: These molecular and ultrastructural benefits position Spirulina platensis as a promising, natural alternative to statins for managing dyslipidemia and preventing statin-induced myopathy. Future translational and clinical studies are warranted to further validate its efficacy and safety, supporting its broader application in metabolic and muscle-related disorders. Full article