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Search Results (1,243)

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Keywords = pulsed heat

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10 pages, 5092 KB  
Article
A Compact Heat Sink Compatible with a Ka-Band Gyro-TWT with Non-Superconducting Magnets
by Shaohang Ji, Boxin Dai, Zewei Wu, Wei Jiang, Xin Chen, Binyang Han, Jianwei Zhou, Qianqian Chen, Guo Liu, Yelei Yao, Jianxun Wang and Yong Luo
Quantum Beam Sci. 2026, 10(1), 4; https://doi.org/10.3390/qubs10010004 - 22 Jan 2026
Viewed by 46
Abstract
This paper presents a thermal management solution for a Ka-band gyrotron traveling wave tube (gyro-TWT) with non-superconducting magnets. At present, the miniaturization and non-superconductivity of gyro-TWT have become a trend, but miniaturization leads to a significant increase in power density and a severe [...] Read more.
This paper presents a thermal management solution for a Ka-band gyrotron traveling wave tube (gyro-TWT) with non-superconducting magnets. At present, the miniaturization and non-superconductivity of gyro-TWT have become a trend, but miniaturization leads to a significant increase in power density and a severe limitation in heat sink volume, which critically limits power capacity. To address this challenge, a joint microwave–thermal management evaluation model is used to investigate the heat transfer process and identify the crucial factors constraining the power capacity. A cylindrical heat sink with narrow rectangular grooves is introduced. Based on this, the cooling efficiency has been enhanced through structural optimization. The beam–wave interaction, electrothermal conversion, and heat conduction processes of the interaction circuit are analyzed. The compact heat sink achieves a 1.2-fold increase in coolant utilization and reduces the overall volume by 27.4%. Meanwhile, this heat sink improves the cooling performance and power capability of the gyro-TWT effectively. At 29 GHz, the gyro-TWT achieves a pulse power of 150 kW. Simulation results show that the maximum temperature is 348 °C at a 45% duty cycle, reduced by 159 °C. The power capacity of the Ka-band gyro-TWT increases by 40.6%. Full article
(This article belongs to the Section Radiation Scattering Fundamentals and Theory)
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17 pages, 5957 KB  
Article
Precision Cutting of CF/PEEK by UV Nanosecond Laser for On-Orbit Manufacturing Applications
by Wenqiang Wu, Bing Wei, Yu Huang and Congyi Wu
Micromachines 2026, 17(1), 93; https://doi.org/10.3390/mi17010093 - 11 Jan 2026
Viewed by 313
Abstract
On-orbit cutting is a critical process for the on-orbit manufacturing of carbon fiber reinforced polyetheretherketone composites (CF/PEEK) truss structures, with pulsed laser cutting serving as one of the feasible methods. Achieving high-quality cutting of CF/PEEK remains a major challenge for on-orbit manufacturing. Therefore, [...] Read more.
On-orbit cutting is a critical process for the on-orbit manufacturing of carbon fiber reinforced polyetheretherketone composites (CF/PEEK) truss structures, with pulsed laser cutting serving as one of the feasible methods. Achieving high-quality cutting of CF/PEEK remains a major challenge for on-orbit manufacturing. Therefore, the cutting process of CF/PEEK prepreg tape was studied by an ultraviolet (UV) nanosecond laser. A three-factor, five-level orthogonal experiment was carried out to analyze the influence of laser repetition rate (LRR), laser cutting speed (LCS), and laser scanning times (LCTs) on cutting quality. The ablation mechanism dominated by the photothermal effect between the UV nanosecond laser and CF/PEEK was analyzed, and the by-products in the cutting process were explored. Finally, the optimal cutting quality (the width of slit (Ws) = 41.69 ± 3.54 μm, the heat-affected zone (HAZ) = 87.27 ± 7.30 μm) was obtained under the process conditions of LRR 50 kHz-LCS 50 mm/s-LCT 16 times. The findings show that the WS and HAZ increase with the increase in LRR and LCT and the decrease in LCS, and the carbon fiber decomposes and escapes due to the photothermal effect. Full article
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44 pages, 1670 KB  
Review
Synergistic Interactions Between Bacteria-Derived Metabolites and Emerging Technologies for Meat Preservation
by Carlos Alberto Guerra, André Fioravante Guerra and Marcelo Cristianini
Fermentation 2026, 12(1), 43; https://doi.org/10.3390/fermentation12010043 - 10 Jan 2026
Viewed by 610
Abstract
Considering the challenges associated with implementing emerging technologies and bacterial-derived antimicrobial metabolites at an industrial scale in the meat industry, this comprehensive review investigates the interactions between lactic acid bacteria-producing antimicrobial metabolites and emerging food preservation technologies applied to meat systems. By integrating [...] Read more.
Considering the challenges associated with implementing emerging technologies and bacterial-derived antimicrobial metabolites at an industrial scale in the meat industry, this comprehensive review investigates the interactions between lactic acid bacteria-producing antimicrobial metabolites and emerging food preservation technologies applied to meat systems. By integrating evidence from microbiology, food engineering, and molecular physiology, the review characterizes how metabolites-derived compounds exert inhibitory activity through pH modulation, membrane permeabilization, disruption of proton motive force, and interference with cell wall biosynthesis. These biochemical actions are evaluated in parallel with the mechanistic effects of high-pressure processing, pulsed electric fields, cold plasma, irradiation, pulsed light, ultrasound, ohmic heating and nanotechnology. Across the literature, consistent patterns of synergy emerge: many emerging technologies induce structural and metabolic vulnerabilities in microbial cells, thereby amplifying the efficacy of antimicrobial metabolites while enabling reductions in process intensity. The review consolidates these findings to elucidate multi-hurdle strategies capable of improving microbial safety, extending shelf life, and preserving the physicochemical integrity of meat products. Remaining challenges include optimizing combinational parameters, ensuring metabolite stability within complex matrices, and aligning integrated preservation strategies with regulatory and industrial constraints. Full article
(This article belongs to the Special Issue Microbial Fermentation: A Sustainable Approach to Food Production)
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18 pages, 4905 KB  
Article
Enhancing Spore Inactivation: Low-Intensity Pulsed Electric Field Combined with Ohmic Heating and Germinant Pretreatment
by Fei-Yue Xu, Hua-Xi Huang, Qing-Hui Wen, Lang-Hong Wang, Yan-Yan Huang and Man-Sheng Wang
Foods 2026, 15(2), 230; https://doi.org/10.3390/foods15020230 - 8 Jan 2026
Viewed by 243
Abstract
Bacterial spores, as one of the most resistant microbial forms, are difficult to completely eliminate through conventional heat treatments such as pasteurization, allowing them to persist in food and pose a significant threat to microbial safety. This study employed a “germination–inactivation” strategy to [...] Read more.
Bacterial spores, as one of the most resistant microbial forms, are difficult to completely eliminate through conventional heat treatments such as pasteurization, allowing them to persist in food and pose a significant threat to microbial safety. This study employed a “germination–inactivation” strategy to inactivate Alicyclobacillus acidoterrestris (AAT) spores using a germinant under low-intensity pulsed electric fields (PEFs). Analysis of germination curves identified 40 mM L-valine as the most effective germinant. Results showed that after 4-h incubation with 40 mM L-valine followed by 210 s of 0.18 kV/cm PEF treatment, the synergistic effect of electric field and ohmic heating (OH) reduced AAT spore counts by 1.73 log units. In contrast, the control group treated with the same PEF parameters without a germinant showed only a 0.54 log unit reduction. These findings indicate that germination agents significantly reduce spore resistance. Subsequent experiments confirmed that L-valine-treated AAT spores underwent pronounced structural disruption under the combined effects of the electric field and OH, leading to leakage of intracellular components such as nucleic acids and proteins. This phenomenon was verified via scanning electron microscopy (SEM) and laser confocal microscopy. Additionally, both ROS levels and ATPase activity in spores were substantially reduced, further indicating that the combined electric field and OH synergistically disrupted the spore’s external structure and internal macromolecules, leading to spore death. Thus, low-intensity PEF assisted by spore germination agents offers an energy-efficient and effective inactivation method, opening new avenues for spore inactivation research. Full article
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11 pages, 241 KB  
Article
The Role of High-Flow Nasal Cannula (HFNC) During Flexible Bronchoscopy in Adult Patients with Moderate Respiratory Dysfunctions: An Observational Study
by Francesco Coppolino, Pasquale Sansone, Gianluigi Cosenza, Simona Brunetti, Francesca Piccialli, Marco Fiore, Clelia Esposito, Maria Caterina Pace and Vincenzo Pota
J. Clin. Med. 2026, 15(2), 459; https://doi.org/10.3390/jcm15020459 - 7 Jan 2026
Viewed by 255
Abstract
Background/Objectives: Flexible bronchoscopy (FB) enables airway exploration and diagnosis of various respiratory pathologies, but the sedation and instrumentation required during the procedure raise oxygen demand while reducing ventilation, which can lead to hypoxemia. Conventional oxygen therapy (COT) may not adequately prevent desaturations [...] Read more.
Background/Objectives: Flexible bronchoscopy (FB) enables airway exploration and diagnosis of various respiratory pathologies, but the sedation and instrumentation required during the procedure raise oxygen demand while reducing ventilation, which can lead to hypoxemia. Conventional oxygen therapy (COT) may not adequately prevent desaturations in high-risk groups, as patients with moderate respiratory deficiency. High-flow nasal cannula (HFNC) can deliver heated, humidified oxygen at high flow rates, generating low-level positive airway pressure, improving oxygenation, reducing dead-space, and enhancing procedure tolerance. Prior studies have shown that HFNC can improve gas exchange and reduce desaturations during bronchoscopy. However, evidence remains limited for patients with moderate respiratory deficiency, who are particularly vulnerable. Evaluating the feasibility and safety of HFNC in this population is essential to guide safe procedural practice. Methods: A retrospective observational study including patients undergoing FB with HFNC support between January and May 2025. Inclusion criteria were BMI between 18 and 30; age > 18 years old; moderate respiratory dysfunction, defined by pulse oximetry, Pulmonary Functional Tests (PFTs) and Arterial Blood Gas (ABG) analysis. Exclusion criteria were intolerance/contraindication to HFNC. Procedures were performed under basic monitoring. Primary outcome was occurrence of severe hypoxemia (SpO2 < 90%). Secondary outcomes were needed for rescue maneuvers, interruption for conversion to other ventilatory strategies, and hemodynamic instability. Results: No severe desaturations were recorded, all procedures were completed without rescue maneuvers or other ventilatory strategies, and no hypoxemia occurred. Mean duration of the procedure was 9 min. Vital parameters were maintained within the normal ranges, with a mean SpO2 during bronchoscopy of 98%. Conclusions: HFNC enables oxygenation and ventilation without adverse events in sedations for FB in patients with moderate respiratory deficiency. Full article
23 pages, 6219 KB  
Article
Functional Nanocomposites with a Positive Temperature Coefficient of Resistance Based on Carbon Nanotubes Synthesized by Laser Ablation
by Alexandr V. Shchegolkov, Aleksei V. Shchegolkov, Ivan D. Parfimovich, Vladimir V. Kaminskii and Mariya Y. Putyrskaya
J. Compos. Sci. 2026, 10(1), 19; https://doi.org/10.3390/jcs10010019 - 4 Jan 2026
Viewed by 307
Abstract
This study presents the development of high-performance polymer composites designed for operation under extreme conditions. The research aimed to investigate the influence of laser ablation parameters on the synthesis of carbon nanotubes (CNTs) and to evaluate their efficacy as electrically conductive fillers. CNTs [...] Read more.
This study presents the development of high-performance polymer composites designed for operation under extreme conditions. The research aimed to investigate the influence of laser ablation parameters on the synthesis of carbon nanotubes (CNTs) and to evaluate their efficacy as electrically conductive fillers. CNTs were synthesized using a 200 W laser ablation setup, with the graphite-to-ferrocene ratio in the target varied from 3:1 to 8:1 at a constant pulse duration of 0.1 s. Comprehensive analysis by Raman spectroscopy and scanning electron microscopy (SEM) demonstrated that this method enables the production of nanotubes with controlled morphology and diameters ranging from 20 to 70 nm. It was established that varying the target composition serves as an effective tool for managing the specific surface area and structure of the synthesized CNTs. The obtained nanotubes exhibited high efficiency in forming conductive networks within polymer matrices (exemplified by silicone), thereby imparting the composites with tailored electrophysical properties. A key finding of the work is the identified dependence of the positive temperature coefficient of resistance (PTCR) of the composites on the morphology and composition of the carbon filler. This property opens prospects for creating “smart” self-regulating heating elements based on the developed materials, including for anti-icing systems. Thus, the study results confirm that the targeted synthesis of CNTs via laser ablation and their subsequent incorporation into polymer matrices constitutes an effective strategy for expanding the functional capabilities of composite materials in modern technical applications. Full article
(This article belongs to the Section Nanocomposites)
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10 pages, 624 KB  
Communication
The Effect of Magnetotherapy on Back Pain Sensitivity and Muscle Tension in Recreational Horses—A Pilot Study
by Ewa Jastrzębska, Dominika Dobbek and Aleksandra Pawłowska
Animals 2026, 16(1), 77; https://doi.org/10.3390/ani16010077 - 26 Dec 2025
Viewed by 396
Abstract
Back pain in horses is a frequent musculoskeletal issue that affects performance and welfare. Magnetotherapy has been proposed as a complementary, non-invasive treatment to reduce pain and support soft tissue recovery, but studies in horses remain limited. This pilot study aimed to evaluate [...] Read more.
Back pain in horses is a frequent musculoskeletal issue that affects performance and welfare. Magnetotherapy has been proposed as a complementary, non-invasive treatment to reduce pain and support soft tissue recovery, but studies in horses remain limited. This pilot study aimed to evaluate the effects of low-frequency pulsed magnetic field therapy on horses with hypersensitivity to palpation along the longissimus dorsi muscle. Four recreational horses participated in a 10-session magnetotherapy program, with changes assessed using palpation, neck flexibility tests, heart rate measurements and thermal imaging. Results showed a reduction in pain sensitivity and muscle tension, particularly in the withers, thoracic, lumbar and sacral regions. Heart rate decreased after treatment, which may indicate a relaxing effect. Thermal imaging confirmed that magnetotherapy did not increase surface temperature, confirming its non-thermal nature. No adverse effects or swelling were observed in any of the horses. These findings provide preliminary data from this pilot study, suggesting that magnetotherapy may be a beneficial adjunct in the treatment of back pain in horses, promoting relaxation and pain relief without inducing tissue heating. Further research on larger populations with a negative control group is needed to validate these findings and support broader clinical application. Full article
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12 pages, 2336 KB  
Article
Estimation of Soil Water Flux Using the Heat Pulse Technique and Vector Addition in Saturated Soils of Different Textures
by Fuyun Lu, Zhi Zhao, Qinghua Pan, Yuping Zhang, Dongye Lu and Yang Wu
Water 2026, 18(1), 67; https://doi.org/10.3390/w18010067 - 25 Dec 2025
Viewed by 444
Abstract
Soil water flux is a key parameter for understanding water and heat transport processes in the vadose zone. The heat pulse technique (HPT) has shown considerable potential for predicting soil water flux. Traditional three-needle probe methods, the maximum dimensionless temperature difference (MDTD [...] Read more.
Soil water flux is a key parameter for understanding water and heat transport processes in the vadose zone. The heat pulse technique (HPT) has shown considerable potential for predicting soil water flux. Traditional three-needle probe methods, the maximum dimensionless temperature difference (MDTD) method and the ratio of downstream to upstream temperature increases (Ratio) method, can only measure water flux along the probe alignment. To enhance the applicability of the HPT method, the five-needle probe with vector addition allows for the measurement of soil water flux in any direction within the plane perpendicular to the needles. However, its applicability across different soil textures remains unclear. The objective of this study was to evaluate the applicability of the MDTD and Ratio methods when combined with vector addition across different soil textures. Experimental results show that the vector MDTD and Ratio methods improve water flux measurement accuracy compared with traditional three-needle methods, confirming the reliability of the vector HPT approach. Specifically, the mean absolute percentage error (MAPE) of the vector MDTD method decreased by 1.69%, 1.04%, and 1.80% in sand, sandy loam, and silt loam, respectively, compared with the traditional MDTD method. In contrast, the MAPE of the vector Ratio method varied by +8.83%, −6.73%, and −18.20% in the same soils, relative to the traditional Ratio method. Examining the root mean square error (RMSE) of each method yields a similar conclusion. Similarly to traditional HPT methods, the measurement accuracy of the vector HPT approach is influenced by soil texture, water flux range, and probe spacing. Notably, because the vector HPT method involves four probe spacings, namely the distances between the heating needle and the temperature-sensing needles, it can exacerbate the instability of the resultant water flux measurements. These findings may facilitate the broader application of the HPT method. Full article
(This article belongs to the Section Soil and Water)
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12 pages, 1593 KB  
Article
Assessment of the Self-Heating Potential of Fresh Wood Using the Pulse Flow Calorimetric Method
by Boleslav Taraba
Fire 2026, 9(1), 12; https://doi.org/10.3390/fire9010012 - 24 Dec 2025
Viewed by 406
Abstract
The self-heating propensity of the fresh wood of ten tree species (two coniferous, eight deciduous) was studied calorimetrically using oxidation heats, q30, at a temperature of 30 °C. Values of q30 in the range between 0.45 W kg−1 (dry) [...] Read more.
The self-heating propensity of the fresh wood of ten tree species (two coniferous, eight deciduous) was studied calorimetrically using oxidation heats, q30, at a temperature of 30 °C. Values of q30 in the range between 0.45 W kg−1 (dry) and 1.1 W kg−1 (dry) were found. The lowest evolution of the oxidation heat proved two coniferous wood types—spruce and pine. On the other hand, the highest value of the q30 heat manifested willow wood, which exceeded (as the only one of the samples) the level of 1 W kg−1 (dry). Water was confirmed to promote the generation of oxidation heat, while completely negligible oxidation heat effects were found in dry wood samples. A rise in the heat evolution with increasing moisture content can be explained not only by a change in the mechanistic pathway of the chemical oxidation of wood in the presence of water, but also by the restoration of the activity of microorganisms in wood, which occurs only at a sufficient level of moisture content. Tree bark appears to be probable carrier of a diverse microbiome. Based on the experiments with debarked wood samples, it can be estimated that the part of the heat produced by microorganisms constitutes a remarkable 35–55% of the global oxidation heat q30, as determined for fresh wood samples. Full article
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23 pages, 6534 KB  
Article
Modeling and Simulation of Multi-Pulse Femtosecond Laser Ablation of WC-6Co Cemented Carbide
by Jin Wang, Haijiao Xu, Shiwei Zhang and Lianyu Fu
Micromachines 2026, 17(1), 11; https://doi.org/10.3390/mi17010011 - 23 Dec 2025
Viewed by 312
Abstract
In the process of femtosecond pulsed laser machining of cemented carbide, the simulation study of multi-pulse laser ablation is of great significance in revealing the laser ablation effect and guiding the machining process. When performing modeling, the consideration of the two-temperature equation, cumulative [...] Read more.
In the process of femtosecond pulsed laser machining of cemented carbide, the simulation study of multi-pulse laser ablation is of great significance in revealing the laser ablation effect and guiding the machining process. When performing modeling, the consideration of the two-temperature equation, cumulative effect, and the improvement in the energy attenuation term in the laser heat source have a great influence on the simulation accuracy, but there are few related studies. This study aims to solve the above three problems and establish a more accurate finite element simulation model to reveal the effect of femtosecond laser ablation of cemented carbide. In order to apply the dual-temperature equation, the thermophysical parameters are chosen to approximate similar materials through experiments; in order to consider the cumulative effect, the accumulation coefficients are obtained through experiments and corrected based on the energy accumulation model for the laser heat source. In order to consider the influence of multi-pulse laser processing on the energy decay term in the traditional laser body heat source, the energy decay term is expanded by transforming the starting surface of the attenuation. The multi-pulse laser two-temperature model is established based on the WC-6Co material, the temperature field distribution and ablation morphology of the sample surface under the action of femtosecond laser are obtained, and the accuracy of the simulation model is verified by experimental results. On this basis, the influence of the average power and the number of pulses of the multi-pulse laser on the ablation effect of the material is quantitatively investigated. Full article
(This article belongs to the Section E:Engineering and Technology)
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11 pages, 1792 KB  
Article
Theoretical Study of Ultra-Fast Laser Lift-Off of Carbon Nanotube-Integrated Polyimide Films
by Run Bai, Yachong Xu, Junwei Fu, Zhenzhen Sun, Yanbo Wang, Rui Yang, Zijuan Han, Fanfan Wang and Boyuan Cai
Nanomaterials 2026, 16(1), 1; https://doi.org/10.3390/nano16010001 - 19 Dec 2025
Viewed by 384
Abstract
In this paper, ultra-fast laser lift-off (LLO) of carbon nanotube (CNT)-integrated polyimide film (PI) was investigated by different laser burst mode and pulse intervals using the two-temperature model. By comparing the temperature field distributions of nanosecond, picosecond, and femtosecond lasers at different pulse [...] Read more.
In this paper, ultra-fast laser lift-off (LLO) of carbon nanotube (CNT)-integrated polyimide film (PI) was investigated by different laser burst mode and pulse intervals using the two-temperature model. By comparing the temperature field distributions of nanosecond, picosecond, and femtosecond lasers at different pulse intervals, it can be found that picosecond lasers cause a higher lattice temperature increase at the PI interface with specific pulse interval conditions. With the increase in the pulse interval, the lattice temperature of the three kinds of lasers decreased, indicating that the heat accumulation effect was weakened. In addition, under picosecond laser irradiation, the lattice temperature at the PI/glass interface of integrated CNTs could be significantly increased, which was significantly different from the system without integrated CNTs. The simulation results show that the picosecond laser is more suitable for LLO with an appropriate pulse interval, and the integration of CNTs at the PI/glass interface can effectively reduce the laser energy threshold required for the LLO process. Our work presents a new PI/CNT/glass model for ultra-fast laser low-threshold LLO and promotes the laser debonding technology in the fields of OLED and other optoelectronic chips. Full article
(This article belongs to the Special Issue Nano-Optics and Nanophotonics)
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28 pages, 10229 KB  
Article
Mechanical Properties of Copper Tailings Cemented Paste Backfill Incorporating Thermally and Mechanically Treated Saudi Natural Pozzolan
by Ardhymanto Am Tanjung, Haitham M. Ahmed and Hussin A. M. Ahmed
Appl. Sci. 2025, 15(24), 13205; https://doi.org/10.3390/app152413205 - 17 Dec 2025
Viewed by 340
Abstract
Cemented Paste Backfill (CPB) is a technique that utilizes mine tailings, mining-process water, and a binder, typically Ordinary Portland Cement (OPC), to backfill the opening created in underground mining. However, the use of cement in CPB increases operational costs and has adverse environmental [...] Read more.
Cemented Paste Backfill (CPB) is a technique that utilizes mine tailings, mining-process water, and a binder, typically Ordinary Portland Cement (OPC), to backfill the opening created in underground mining. However, the use of cement in CPB increases operational costs and has adverse environmental effects. To mitigate these effects, eco-friendly natural pozzolan can be used as a partial replacement for OPC, thereby reducing its consumption and environmental impact. The volcanic region of western Saudi Arabia contains extensive deposits of Saudi natural pozzolan (SNP), which is a promising candidate for this purpose. This study evaluates the mechanical performance of CPB under four scenarios: a control mixture (CTRL), a mixture with untreated SNP (UT), and mixtures with activated SNP, specifically heat-treated (HT) and mechanically treated (MT). Each scenario was tested at replacement levels of 5%, 10%, 15%, and 20% of OPC. The performance was assessed using Uniaxial Compressive Strength (UCS) with Elastic Modulus (E), Ultrasonic Pulse Velocity (UPV), and Indirect Tensile Strength (ITS/Brazilian) tests. The results indicate that the HT scenario at a 5% replacement level delivered the highest performance, slightly outperforming the MT scenario. Both activated scenarios (HT and MT) significantly surpassed the untreated mixture (UT). Overall, the HT scenario proved to be the most effective among all CPB mixtures tested. XRD diffractogram analysis supported HT as the material with the highest strength performance due to the occurrence of more strength phases than other CPB materials, including Alite, Quartz, and Calcite. While UCS and UPV showed a positive correlation across all CPB materials, the relationship between UPV and the modulus of elasticity (E) demonstrated a low correlation. The findings suggest that using activated SNP materials can enhance CPB sustainability by lowering cement demand, stabilizing operating costs, and reducing environmental impacts. Full article
(This article belongs to the Special Issue Mining Engineering: Present and Future Prospectives)
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15 pages, 3499 KB  
Article
Photothermal Heat Transfer in Nano-Hydroxyapatite/Carbon Nanotubes Composites Modeled Through Cellular Automata
by Cecilia Mercado-Zúñiga and José Antonio García-Merino
Crystals 2025, 15(12), 1062; https://doi.org/10.3390/cryst15121062 - 17 Dec 2025
Viewed by 314
Abstract
Modeling elementary diffusion processes in nanostructured materials is essential for developing platforms capable of interacting with high-speed physical signals. In this work, the photothermal response of a nano-hydroxyapatite/carbon nanotube (nHAp/CNT) composite was experimentally characterized and modeled through a cellular automaton (CA) framework designed [...] Read more.
Modeling elementary diffusion processes in nanostructured materials is essential for developing platforms capable of interacting with high-speed physical signals. In this work, the photothermal response of a nano-hydroxyapatite/carbon nanotube (nHAp/CNT) composite was experimentally characterized and modeled through a cellular automaton (CA) framework designed to capture the thermal propagation of the hybrid system. Synthesizing nHAp/CNT composites enables the combination of the biocompatible and piezoelectric nature of nHAp with the enhanced photothermal response introduced by CNTs. UV–Vis reflectance measurements confirmed that CNT incorporation increases the optical absorption of the ceramic matrix, resulting in more efficient photothermal conversion. The composite was irradiated with a nanosecond pulsed laser, and the resulting thermal transients were compared with CA simulations based on a D2Q9 lattice configuration. The model accurately reproduces experiments, achieving R2 > 0.991 and NRMSE below 2.4% for all tested laser powers. This strong correspondence validates the CA approach for predicting spatiotemporal heat diffusion in heterogeneous nanostructured composites. Furthermore, the model revealed a sensitive thermal coupling when two heat sources were considered, indicating synergistic enhancement of local temperature fields. These findings demonstrate both the effective integration of CNTs within the nHAp matrix and the capability of CA-based modeling to describe their photothermal behavior. Overall, this study establishes a computational–experimental basis for designing controlled thermal-wave propagation and guiding future multi-frequency or multi-source photothermal mixing experiments. Full article
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21 pages, 2751 KB  
Article
Temperature-Dependent Recombinase-Based Genetic Circuits
by Marc Gonzalez-Colell, Mariana Gomes del Castillo, Marta Palau Gauthier and Javier Macia
Int. J. Mol. Sci. 2025, 26(24), 12055; https://doi.org/10.3390/ijms262412055 - 15 Dec 2025
Viewed by 365
Abstract
Temperature offers a simple yet powerful signal to program cellular behavior. Here, we engineered and characterized a set of temperature-dependent genetic circuits that integrate RNA thermometers with site-specific DNA recombinases to achieve precise, irreversible control of gene expression. Using the serine recombinase Bxb1 [...] Read more.
Temperature offers a simple yet powerful signal to program cellular behavior. Here, we engineered and characterized a set of temperature-dependent genetic circuits that integrate RNA thermometers with site-specific DNA recombinases to achieve precise, irreversible control of gene expression. Using the serine recombinase Bxb1 placed under the control of the Salmonella FourU RNA thermometer, we demonstrate how promoter strength critically defines thermal sensitivity: weak promoters’ activity clears ON/OFF transitions, while strong promoters lead to continuous, quasi-temperature-independent recombination. Furthermore, temperature pulse duration and growth phase of cell culture were found to modulate recombination efficiency, providing additional layers of control. We illustrate the potential of this framework through proof-of-concept applications, including (i) the generation of spatial expression patterns on 2D surfaces via localized heating, (ii) a paper-based device capable of recording temperature gradients as stable genetic outputs, and (iii) a temperature-triggered lysis system for controlled cellular release. Together, these results establish temperature-regulated recombinase circuits as versatile and robust tools for programmable, spatially resolved, and irreversible control of gene expression, paving the way for new applications in synthetic biology, biosensing, and bioproduction. Full article
(This article belongs to the Section Biochemistry)
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27 pages, 13345 KB  
Article
Micro and Macrostructural Assessment of Welded 6082 Aluminium Alloy T-Connections
by Darko Landek, Ivica Garašić, Davor Skejić, Anđelo Valčić, Ivan Čudina and Mislav Štefok
Metals 2025, 15(12), 1365; https://doi.org/10.3390/met15121365 - 11 Dec 2025
Viewed by 435
Abstract
One of the main challenges in welding aluminium concerns structural integrity and a significant reduction in mechanical properties in the region adjacent to the weld. Design provisions can result in a drastic reduction, which may exceed 50% of the base metal resistance. This [...] Read more.
One of the main challenges in welding aluminium concerns structural integrity and a significant reduction in mechanical properties in the region adjacent to the weld. Design provisions can result in a drastic reduction, which may exceed 50% of the base metal resistance. This research aims to evaluate the accuracy of the HAZ extent values codified in Eurocode 9 for T-connections fabricated from artificially aged 6082 aluminium alloy, which is widely used in load-bearing structures. Three plate thicknesses (6, 8 and 10 mm) and two pulsed MIG welding processes (DC-MIG-P and AC-MIG-P) were used to fabricate 20 T-connection specimens (10 different configurations) in accordance with EN 1090-3. The study focuses on characterising the welding zones through hardness testing and metallographic examination. Results show that AC-MIG-P offers better control over thermal input and may reduce structural distortion, while DC-MIG-P provides more robust fusion and metallurgical continuity. Findings related to HAZ extent (12.77 mm and 15.36 mm maximum measured for AC-MIG-P and DC-MIG-P, respectively) suggest that Eurocode 9 may be overly conservative for pulsed MIG welding processes, particularly for greater plate thicknesses where a HAZ extent of 22.50 mm or more is specified. Consequently, adopting more precise, process-specific HAZ characterisations could lead to more realistic connection design and structural behaviour. Full article
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