Search Results (444)

Mycoplasma bovis is an important pathogen that is associated with respiratory diseases, mastitis, and arthritis in cattle, leading to significant economic losses in the global cattle industry. Most notably in this study, we pioneer the discovery that its secreted effector ENO1 (α-enolase) directly targets host cytoskeletal proteins for metabolic–immune regulation. Using an innovative GST pull-down/mass spectrometry approach, we made the seminal discovery of β-actin (ACTB) as the primary host target of ENO1—the first reported bacterial effector–cytoskeleton interaction mediating metabolic reprogramming. ENO1–ACTB binding depends on a hydrogen bond network involving ACTB’s 117Glu and 372Arg residues. This interaction triggers (1) glycolytic activation via Glut1 upregulation, establishing Warburg effect characteristics (lactic acid accumulation/ATP inhibition), and (2) ROS-mediated activation of dual inflammatory axes (HIF-1α/IL-1β and IL-6/TNF-α). This work establishes three groundbreaking concepts: (1) the first evidence of a pathogen effector hijacking host ACTB for metabolic manipulation, (2) a novel ‘glycolysis–ACTB–ROS-inflammation’ axis, and (3) the first demonstration of bacterial proteins coordinating a Warburg effect with cytokine storms. These findings provide new targets for anti-infection therapies against Mycoplasma bovis. Full article

In this study, three alternating donor–acceptor (D–A) type [12]cycloparaphenylene ([12]CPP) derivatives ([12]CPP 1a, 2a, and 3a) were designed through precise molecular engineering, and their multidimensional photophysical responses and chiroptical properties were systematically investigated. The effects of the alternating D–A architecture on electronic structure, excited-state dynamics, and optical behavior were elucidated through density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations. The results show that the alternating D–A design significantly reduced the HOMO–LUMO energy gap (e.g., 3.11 eV for [12]CPP 2a), enhanced charge transfer characteristics, and induced pronounced red-shifted absorption. The introduction of an imide-based acceptor ([12]CPP 2a) further strengthened the electron push-pull interaction, exhibiting superior performance in two-photon absorption, while the symmetrically multifunctionalized structure ([12]CPP 3a) predominantly exhibited localized excitation with the highest absorption intensity but lacked charge transfer features. Chiral analysis reveals that the alternating D–A architecture modulated the distribution of chiral signals, with [12]CPP 1a displaying a strong Cotton effect in the low-wavelength region. These findings not only provide a theoretical basis for the molecular design of functionalized CPP derivatives, but also lay a solid theoretical foundation for expanding their application potential in optoelectronic devices and chiral functional materials. Full article

Continuous SiC fiber-reinforced SiC matrix composites (SiC/SiC), as structural heat protection integrated materials, are often used in parts for large-area heat protection and sharp leading edges, and there are a variety of low-velocity impact events in their service. In this paper, a drop hammer impact test was conducted using narrow strip samples to simulate the low-velocity impact damage process of sharp-edged components. During the test, different impact energies and impact times were set to focus on investigating the low-velocity impact damage characteristics of 2.5D SiC/SiC composites. To further analyze the damage mechanism, computed tomography (CT) was used to observe the crack propagation paths and distribution states of the composites before and after impact, while scanning electron microscopy (SEM) was employed to characterize the differences in the micro-morphology of their fracture surfaces. The results show that the in-plane impact behavior of a 2.5D needled SiC/SiC composite strip samples differs from the conventional three-stage pattern. In addition to the three stages observed in the energy–time curve—namely in the quasi-linear elastic region, the severe load drop region, and the rebound stage after peak impact energy—a plateau stage appears when the impact energy is 1 J. During the impact process, interlayer load transfer is achieved through the connection of needled fibers, which continuously provide significant structural support, with obvious fiber pull-out and debonding phenomena. When the samples are subjected to two impacts, damage accumulation occurs inside the material. Under conditions with the same total energy, multiple impacts cause more severe damage to the material compared to a single impact. Full article

Introduction: Strength training, essential for health and performance, often uses free weights for greater stabilization demands and pulleys for easier load adjustment and progression. Methods: The aim of the study was to analyze the differences in force application using gravitational and pneumatic resistances. Twenty experienced subjects participated in the study (age: 21.9 ± 3.8 years; body mass: 76.3 ± 9.4 kg; height: 177.4 ± 7.5 cm), performing four exercises with each type of resistance: bench press, lat pulldown, chest fly, and single-arm row. The participants performed 8 repetitions per exercise. Peak and mean force were measured with a 100 Hz load cell (SUIFF S2 Pro) during the concentric phase of the lifts. Differences between resistance types were analyzed using one-way ANOVA and paired t-tests. Results: Peak force was higher with gravitational resistance across all exercises (p < 0.001; d = 2.1–4.7). Average force with gravitational resistance was also higher in the bench press and lat pulldown (p < 0.05; d = 0.7–1.4), but not in the chest fly or single-arm row. Conclusions: Gravitational resistance may better enhance peak strength, while pneumatic resistance supports consistent force and neuromuscular control. These results allow us to select the resistance type based on specific mechanical characteristics. Full article

The purpose of this study is to reveal the characteristics of wine tourists by determining their demographic and psychographic profiles, the effects of their travel motivations and lifestyles on their attitudes towards wine tourism, and the relationships between their demographic characteristics and attitudes. Based on a quantitative approach, study data were collected using a questionnaire. The sample consists of tourists who had visited a winery or participated in the vintage in a wine destination. Wine tourists’ principal motivations were pull factors and wine-related motivations. Their attitudes towards wine consumption had higher mean values than attitudes towards info-seeking, meaning that wine tourists preferred to taste wine and shop from wineries more than learn about wine. Regarding lifestyles, they were mostly innovators and experiencers. For destinations like Türkiye, wine tourism is a significant economic and sociocultural development tool. It is the first study to identify the wine tourists’ profile based on VALS-2, thereby providing a different perspective for the literature. Full article

Background/Objectives: Deceleration can cause liver ruptures via ligament traction, with a specific, little-known transverse rupture in segment II of the left lobe being a concern. This study aimed to provide a detailed morphological characterization of these segment II ruptures, analyse their formation mechanisms using autopsy material, and propose a systematic intraoperative assessment method to improve their detection. Methods: This study analysed the autopsy cases of 132 victims of sudden, violent deceleration (falls from height, traffic accidents) performed between 2011 and 2014. Liver injuries were meticulously described, focusing on the morphological characteristics of ruptures (course, shape, depth) and their location relative to hepatic ligaments. Cases with prior liver resection due to injuries were excluded. Results: Liver ruptures were found in 61 of the 132 analysed cases (46.2%). A “new location” for ruptures was identified on the diaphragmatic surface of the left lobe’s segment II, near and along the left coronary and triangular ligaments. This specific type of rupture was found in 14 cases. Overall, 40 cadavers had liver ruptures near ligaments, totalling 55 such distinct ruptures, indicating that some had multiple ligament-associated tears. The incidence of liver rupture at this newly described site was statistically significant. Conclusions: Transverse rupture of the left hepatic lobe’s segment II, in its subdiaphragmatic area, results from ligament “pulling” forces during deceleration and is a characteristic injury. Its presence should be considered following blunt abdominal trauma involving deceleration, and the subdiaphragmatic area of the left lateral lobe requires intraoperative inspection, especially if other ligament-associated liver ruptures are found. Full article

Maximal and explosive strength—defined as the ability to rapidly generate high levels of force—are widely recognized as critical for performance in strength–power sports such as track and field throwing. However, their interrelationship remains insufficiently examined, particularly in the upper body of elite athletes. This study examined the relationship between early force production (≤250 ms, subdivided into early phase: 0–100 ms; late phase: 100–250 ms) and peak isometric upper-body push and pull force in elite Swedish track and field throwers. A total of 30 athletes (17 females, 13 males; aged 18–34 years), all competing nationally or internationally in discus, hammer, shot put, or javelin, participated in a cross-sectional assessment. Isometric force was measured during bench press (push) and supine bench row (pull) using a custom-built device. Force output was recorded at 50, 100, 150, 200, and 250 ms, along with peak force. The results showed a progressive increase in the correlation between force at early time points and peak force. Associations were weak to moderate at 50–100 ms (r = 0.07–0.55) and became strong to very strong at 150–250 ms (r = 0.64–0.92). These patterns were consistent across sexes and test types. The findings suggest that maximal strength becomes increasingly important as force production time extends beyond 100 ms. Coaches may benefit from assessing both early and peak force characteristics to inform strength profiling and guide training focus, though further research is needed to determine their impact on performance. Full article

In the present study, the mechanical properties of high-strength steel rebar with different crossrib spacing that affect the bond behavior between steel rebar and concrete is investigated. To reveal the effects of crossrib spacing on the bond behavior of 630 MPa high-strength steel rebar (T63) in concrete, 42 bonding specimens were designed using T63 rebars and T63 rebars with increased crossrib spacing (TB63). The bond properties of two kinds of steel rebar with concrete were investigated by pull-out test and the failure modes, bond strengths, relative slippages, and bond-slip curves were obtained. Based on analysis of bond-slip curves, the applicability of the existing bond-slip constitutive model to describe T63 and TB63 rebars was discussed. It was found that 30–50% increase in crossrib spacing had little effect on the bond failure mode and bond strength of T63 rebar. The bond-slip curves of the two types of bonding specimens were similar and there is a 1.3 to 1.5-fold increase in peak slippage with TB63. The calculation method of critical bond length in Chinese code (GB 50010-2010) is applicable to T63 and TB63 rebars, and the bond-slip characteristics of T63 rebar with different crossrib spacings was reliably described by the bond-slip constitutive model. The research results can be used as the basis for the application of T63 reinforcement and can also be used as a reference for optimizing of rebar ribs outline. Full article

In the field of the wood industry, the competitive effect caused by interregional resource differences and the linkage effect generated by industrial chain synergy profoundly affects the development direction of the industry in each province. Based on China’s input–output table from 2002 to 2017, this paper constructs an industrial comparative advantage measurement model using location entropy and finds that the industrial agglomeration advantage of the wood products industry in Heilongjiang Province is remarkable in the national context, and that it had already caught up with 79% of the provinces in 2017; we analyze the industrial characteristics of the wood products industry in Heilongjiang Province through the upstream and downstream degrees, the backward and forward correlation coefficients. The findings indicate that the average value of the downstream degree is 28.57% higher than the average value of the upstream degree in Heilongjiang Province, and the industry association mode has shifted from “demand-pull” to “supply-led”. Therefore, the timber industry in Heilongjiang Province has the capability to transform its resource advantage into a competitive edge across the entire industrial chain. Meanwhile, its sensitivity to economic fluctuations in various provinces is increasing significantly. However, there are still notable shortcomings: insufficient capacity to expand the terminal market and integrate the downstream segments of the industrial chain. Full article

Conventional bolts frequently fail under large deformations due to stress concentration in soft rock tunnels. In contrast, yielding bolts incorporate energy-absorbing mechanisms to sustain controlled plastic deformation. This study employed FLAC3D to numerically investigate the pull-out, shear, and bending behaviors of yielding bolts, evaluating their support effectiveness in soft rock tunnels. Three-dimensional finite difference models incorporating nonlinear coupling springs and the Mohr–Coulomb criterion were developed to simulate bolt–rock interactions under multifactorial loading. Validation against experimental pull-out tests and field measurements confirmed the model accuracy. Under pull-out loading, the axial forces in yielding bolts decreased more rapidly along the bolt length, reducing stress concentration at the head. The central position of the maximum load-bearing capacity in conventional bolts fractured under tension, resulting in an hourglass-shaped axial force distribution. Conversely, the yielding bolts maintained yield strength for an extended period after reaching it, exhibiting a spindle-shaped axial force distribution. Parametric analyses reveal that bolt spacing exerts a greater influence on support effectiveness than length. This study bridges critical gaps in understanding yielding bolt behavior under combined loading and provides a validated framework for optimizing energy-absorbing support systems in soft rock tunnels. Full article

With growing emphasis on sustainable construction, fiber-reinforced polymer (FRP) bars are increasingly being used as alternatives to steel rebars due to their high strength-to-weight ratio, corrosion resistance, and environmental benefits. This study has investigated the bond behavior between FRP bars and concrete of different strength grades under dynamic loading conditions. To analyze the microscopic properties of FRP bar surfaces, the study employs a variety of techniques, including scanning electron microscopy (SEM), atomic force microscopy (AFM), and non-contact surface profilometry. In addition, X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) measurements, and energy dispersive spectrometry (EDS) are used to further investigate surface characteristics. The results reveal a direct correlation between the resin surface roughness of FRP bars and their wettability characteristics, which in turn influence the cement hydration process. Pull-out tests under different loading rates and concrete strength grades have been conducted to evaluate the bond–slip behavior and failure modes. The results indicate that bond strength increases with increasing concrete strength. Dynamic pull-out tests further reveal that higher loading rates generate heterogeneous stress fields, which limit the deformation of FRP bars and consequently diminish the contribution of mechanical interlock to interfacial bonding. Full article

This study traces the origin and migration of the Nsit Clan of the Ibibio ethnic nationality, focusing on its distinct characteristics. The origin and migration of the Western Nsit Clan in the Niger Delta of Nigeria constitute a complex historical narrative shaped by a confluence of cultural, economic, and environmental factors. Rooted in pre-colonial times, this study delves into the clan’s ancestral origins, tracing the intricate patterns of migration that have defined its historical trajectory. Drawing from a diverse range of primary sources, including oral traditions, and anthropological findings, this research illuminates the cultural dynamics that propelled the clan’s movement across the Niger Delta region. This study also explores the impact of external influences, such as trade and colonialism, on the migration patterns and socio-political organization of the Western Nsit Clan. By unraveling the historical tapestry of this community, this research contributes valuable insights into the broader understanding of the Niger Delta’s rich and multifaceted history. A significant emphasis is placed on the migration patterns of the Western Nsit Clan, analyzing the push and pull factors that compelled movements within the Niger Delta region. Ultimately, this study aims to contribute to a deeper comprehension of the Western Nsit Clan’s historical trajectory, offering valuable insights into the complexities of migration patterns, cultural evolution, and resilience within the context of the Niger Delta’s diverse tapestry. Full article

The corrosion-induced strength degradation of steel nails poses a critical challenge to the structural integrity of timber connection joints, particularly in hygrothermal environments. Compressed wood nails exhibit hygroscopic expansion characteristics, demonstrating their potential as a sustainable alternative to steel nails in structural connections. However, systematic investigations on their shear performance under cyclic hygrothermal conditions remain limited. This study comparatively analyzed the shear behavior evolution of compressed wood nail and galvanized steel nail connections under wet-dry cycles. Distinct failure mechanisms were observed: wood nail connections exhibited characteristic brittle fracture patterns, whereas steel nail connections demonstrated ductile failure through pull-out deformation with nail bending. Notably, compressed wood nails displayed superior environmental stability, with significantly lower degradation rates in terms of load-bearing capacity (2.8% vs. 22.3%) and stiffness (16.3% vs. 38.0%) than their steel counterparts under identical hygrothermal exposure. These findings provide critical design references and data support for implementing wood-based fasteners in moisture-prone engineering applications. Full article

In this paper, the fixed points of involutions on the moduli space of principal $E_6$-bundles over a compact Riemann surface X are investigated. In particular, it is proved that the combined action of a representative $\sigma$ of the outer involution of $E_6$ with the pull-back action of a surface involution $\tau$ admits fixed points if and only if a specific topological obstruction in $H^2\left(X/\tau ,{\pi }_0\left(E_6^{\sigma }\right)\right)$ vanishes. For an involution $\tau$ with $2k$ fixed points, it is proved that the fixed point set is isomorphic to the moduli space of principal H-bundles over the quotient curve $X/\tau$, where H is either $F_4$ or $PSp(8,\mathbb{C})$ and it consists of $2^{g-k+1}$ components. The complex dimensions of these components are computed, and their singular loci are determined as corresponding to H-bundles admitting non-trivial automorphisms. Furthermore, it is checked that the stability of fixed $E_6$-bundles implies the stability of their corresponding H-bundles over $X/\tau$, and the behavior of characteristic classes is discussed under this correspondence. Finally, as an application of the above results, it is proved that the fixed points correspond to octonionic structures on $X/\tau$, and an explicit construction of these octonionic structures is provided. Full article

Steel slag aggregate (SSA), as a high-performance and sustainable material, has demonstrated significant potential in enhancing the mechanical properties of concrete and improving the bond behavior between reinforcement and the concrete matrix, thereby contributing to the seismic resilience of steel slag concrete columns (SSCCs). Nevertheless, the underlying mechanism through which SSA influences the seismic performance of SSCCs remains insufficiently understood, and current analytical models fail to accurately capture the effects of bond strength on structural behavior. In this study, a comprehensive experimental program comprising central pull-out tests and quasi-static cyclic loading tests was conducted to investigate the influence of SSA on bond strength and the seismic response of SSCCs. Key seismic performance indicators, including the hysteresis curve, equivalent viscous damping ratio, and ductility coefficient, were evaluated. The role of bond strength in governing energy dissipation and ductility characteristics was elucidated in detail. The results indicate that bond strength significantly affects the seismic performance of SSCC components. At an SSA replacement ratio of 40%, the specimens show optimal performance: energy dissipation capacity increases by 11.3%, bond–slip deformation in the plastic hinge region decreases by 10%, and flexural deformation capacity improves by 9% compared to the control group. However, when the SSA replacement exceeds 60%, the performance metrics are similar to those of ordinary concrete, showing no significant advantages. Based on the experimental findings, a modified bond–slip constitutive model for the steel slag concrete–reinforcement interface is proposed. Furthermore, a finite element model incorporating bond–slip effects is developed, and its numerical predictions exhibit strong agreement with the experimental results, effectively capturing the lateral load-carrying capacity and stiffness degradation behavior of SSCCs. Full article