Search Results (29)

Pteridium aquilinum (bracken fern) poses a global threat to biodiversity and to the health of both animals and humans due to its toxic metabolites and aggressive ecological expansion. In northern Spain, particularly in regions of intensive livestock farming, these risks may be exacerbated, calling for urgent assessment and monitoring strategies. In this study, we implemented a multidisciplinary approach to evaluate the toxicological and ecological relevance of P. aquilinum through four key actions: (a) quantification of pterosins A and B in young fronds (croziers) using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS); (b) analysis of in vivo genotoxicity of aqueous extracts using Drosophila melanogaster as a model organism; (c) a large-scale survey of local livestock farmers to assess awareness and perceived impact of bracken; and (d) the development and field application of a drone-based mapping tool to assess the spatial distribution of the species at the regional level. Our results confirm the consistent presence of pterosins A and B in croziers, with concentrations ranging from 0.17 to 2.20 mg/g dry weight for PtrB and 13.39 to 257 µg/g for PtrA. Both metabolite concentrations and genotoxicity levels were found to correlate with latitude and, importantly, with each other. All tested samples exhibited genotoxic activity, with notable differences among them. The farmer survey (n = 212) revealed that only 50% of respondents were aware of the toxic risks posed by bracken, indicating a need for targeted outreach. The drone-assisted mapping approach proved to be a promising tool for identifying bracken-dominated areas and provides a scalable foundation for future ecological monitoring and land management strategies. Altogether, our findings emphasize that P. aquilinum is not merely a local concern but a globally relevant toxic species whose monitoring and control demand coordinated scientific and policy-based efforts. Full article

Under the pressures of global climate change, the sustainable management of plant resources in alpine gorge regions faces severe challenges. P. aquilinum var. latiusculum is widely harvested and utilized by residents in the upper reaches of the Dadu River–Min River basin due to its high edible and medicinal value. This study employed ensemble models to simulate the potential distribution of P. aquilinum var. latiusculum in this region, predicting the impacts of future climate change on its distribution, the centroid migration of suitable habitats, and niche dynamics. A production dynamics model was also constructed to identify current and future potential cultivation areas by integrating ecological suitability and nutritional component synergies. The results show that current high-suitability areas and core cultivation zones of P. aquilinum var. latiusculum are predominantly distributed in patchy, fragmented patterns across the Wenchuan, Li, Mao, Luding, and Xiaojin Counties and Kangding City. Under climate change, the “mountain-top trap effect” drives a significant increase in high-suitability areas and core cultivation zones, while moderate-to-low-suitability areas and marginal cultivation zones decrease substantially. Meanwhile, suitable habitats and cultivation areas exhibit a northward migration trend toward higher latitudes. The most significant changes in suitable area and cultivation zone extent, as well as the most pronounced niche shifts, occur under high-emission climate scenarios. This research facilitates the development of suitability-based management strategies for P. aquilinum var. latiusculum in the study region and provides scientific references for the sustainable utilization of montane plant resources in the face of climate change. Full article

Wildfires, one of the most important ecological disturbances, influence the composition and dynamics of ecosystems all around the world. Changes in fire regimes brought on by climate change are making their effects worse by increasing the frequency and size of fires. This study examined the issue of delayed mortality at the species and community levels, concentrating on Mediterranean forests dominated by Quercus ilex and Quercus suber. This research examined areas lacking spectral recovery following a megafire, which, although relatively small compared to the total burned area, represented significant ecological disturbances. The results highlighted distinct post-fire dynamics at both the woodland and species levels. Q. ilex experienced higher delayed mortality, particularly in areas of lower fire severity (NR), likely due to increased intra-specific competition. Because of its thick bark, which offers stronger fire resistance and encourages regeneration even in high-severity fire zones (HR), Q. suber showed greater resilience. Responses from the shrub layer varied, and some species, such as Pteridium aquilinum and Cytisus villosus, showed post-fire proliferation. To improve our knowledge of ecosystem resilience and guide forest management in fire-prone areas, these findings highlight the intricacy of post-fire ecological processes and the need to integrate species-specific features with more general community-level patterns. Full article

The Cerrado domain, one of the richest on Earth, is among the most threatened in South America due to human activities, resulting in biodiversity loss, altered fire dynamics, water pollution, and other environmental impacts. Monitoring this domain is crucial for preserving its biodiversity and ecosystem services. This study aimed to apply machine learning techniques to classify the main vegetation formations of the Cerrado within the IBGE Ecological Reserve, a protected area in Brazil, using high-resolution PlanetScope imagery from 2021 to 2024. Three machine learning methods were evaluated: Random Forest (RF), Support Vector Machine (SVM), and Extreme Gradient Boosting (XGBoost). A post-processing process was applied to avoid misclassification of forest in areas of savanna. After performance evaluation, the SVM method achieved the highest classification accuracy (overall accuracy of 97.51%, kappa coefficient of 0.9649) among the evaluated models. This study identified five main classes: grassland (GRA), savanna (SAV), bare soil (BS), samambaião (SAM, representing the superdominant species Pteridium esculentum), and forest (FOR). Over the three-year period (2021–2024), SAV and GRA formations were dominant in the reserve, reflecting the typical physiognomies of the Cerrado. This study successfully delineated areas occupied by the superdominant species P. esculentum, which was concentrated near gallery forests. The generated maps provide valuable insights into the vegetation dynamics within a protected area, aiding in monitoring efforts and suggesting potential new areas for protection in light of imminent anthropogenic threats. This study demonstrates the effectiveness of combining high-resolution satellite imagery with machine learning techniques for detailed vegetation mapping and monitoring in the Cerrado domain. Full article

Slavonia is the most developed agricultural region in Croatia. With rich and fertile soils that have enabled the cultivation of a wide variety of fruits, vegetables, and cereals, Slavonia has always met the food needs of its population. Today, the biocultural diversity of local varieties and semi-natural vegetation has irretrievably disappeared. Our aim was to document the remaining local knowledge of plant use in this area through in-depth semi-structured interviews, which were conducted in 2022–2023. All possible aspects of the use of plants and fungi were recorded as food, animal feed, medicine, construction, jewelry, rituals and ceremonies, dyes, etc. The names and uses of local plant varieties were also recorded. The results show 1702 entries—a total of 296 plant taxa from 76 families and 28 fungi from 16 families. The most frequently named plants were: Urtica dioica, Robinia pseudoacacia, Rosa canina, and Sambucus nigra. The plants with the greatest variety of uses were Morus alba, Rosmarinus officinalis, Triticum aestivum, and Zea mays. Interesting uses were identified. The leaves of the ornamental plant Hosta sieboldiana are still used today as food for wrapping meat with rice, the aquatic plant Trapa natans is eaten like chestnuts, and Pteridium aquilinum was once consumed as a vegetable. In addition, Ambrosia artemisiifolia and Sambucus ebulus were given to horses to prevent and avoid blood poisoning. Some forest species had a special significance and were revered or favored. The most frequently mentioned edible fungi were Boletus sp., Cantharellus cibarius, and Lactarius piperatus. Auricularia auricula-judae is the only species stated to have been used exclusively as a raw snack. Evidence of edible use of Sarccoscypha coccinea, which was reported as traditionally consumed in the past, was of particular interest. Despite the modernization and agricultural nature of the region, many interesting uses of plants and fungi were identified. Further efforts should be directed towards documenting this knowledge to facilitate its dissemination in the communities that possess it, or at least to preserve it for future generations. Full article

Fly ashes produced in huge amounts during coal combustion requires proper management. The purpose of this study was to determine the impact of fly ash from burning hard coal used in large doses (250, 500, 1000 and 2000 t ha⁻¹) on soil properties and vegetation of fresh mixed coniferous forest within 43 years from the ash application. The experiment was established in the Podzols in the forest habitat of Czułów, Katowice Forest district, Upper Silesia, Poland. Eight tree species were planted in ridges created by ploughing: Pinus sylvestris, P. nigra, Larix decidua, Betula pendula, Quercus robur, Q., Acer pseudoplatanus and Fagus sylvatica. The changes in soil morphology caused significant transformations in the physical and chemical properties of the soil such as soil texture, pH, macronutrients (P, K and Mg) content and C:N ratio. Increasing of ash doses changed the granulometric composition of the soil levels from loamy sand (250 t/ha⁻¹) to silt loam (2000 t ha⁻¹). Initially, the acidic Podzols were alkalized under the influence of the fly ash and then acidified, possibly due to the impact of accumulated litter layers, and the reaction of organic soil horizons changed from strongly acidic (250–1000 t ha⁻¹) to alkalis (2000 t/ha⁻¹). The macronutrients content increased in proportion to the fly ash dose, but the subsequent acidification resulted in a gradual decrease in the macronutrients share in the soil layers. The value of the C:N ratio grew after the ash application and then it gradually reduced, even by half. The transformations of soil horizons’ properties also increased the capacity of the soil sorption complex (CEC). All these processes led to a change in the trophic status of the habitat expressed by the soil habitat index (SIG) and the initial coniferous forest site can be classified as a mixed forest habitat even with the lowest ash dose used. The composition of plant communities developed forty years after the ash application was similar at the lower ash doses and the most frequent and abundant tree species were L. decidua, P. nigra and P. silvestris. B. pendula was previously co-dominant, but it was eliminated from the tree stands during the experiment. Planted trees characteristic of late stages of succession, such as Q. robur, Q. rubra, F. sylvatica and A. pseudoplatanus either did not survive or remained in very low quantities. The herb and moss layers developed in the process of spontaneous colonization, and together with the trees led to phytostabilisation of the bare substrates. After acidification of the topsoil horizons, the herb layers consisted mostly of coniferous, mixed, and deciduous forest species, and the most frequent or abundant were Lysimachia europea and Pteridium aquilinum. The moss layers were represented by coniferous forest flora. At the ash dose of 2000 t ha⁻¹, Tilia cordata settled in one of the seral stages of spontaneous succession and this species dominated in the community and formed a dense tree stand. After the soil acidification, a shift from calcicole to calcifuge plant strategy took place among species of the herbaceous layer. The transformations of plant communities’ composition occurred in relation to changes in the soil properties. Full article

Bracken fern (Pteridium aquilinum (L.) Kuhn) is ubiquitous and acts as a cosmopolitan weed in pastures and similar environments. Despite its historical uses, it presents risks due to toxicity. This study, conducted in the second half of 2023, aimed to assess the environmental and health hazards of P. aquilinum, primarily focusing on its carcinogenic compound, ptaquiloside. The literature was comprehensively reviewed using diverse databases, including PubMed, Web of Science, Scopus, and Google Scholar. Information was synthesized from original research articles, meta-analyses, systematic reviews, and relevant animal studies. Animals grazing on bracken fern face annual production losses due to toxin exposure. The substantial impact on biodiversity, animal health, and human well-being arises from the presence of ptaquiloside and related compounds in milk, meat, and water, along with the increasing global prevalence of P. aquilinum and its swift colonization in acidic soil and fire-damaged areas. The objectives were to identify major bioactive compounds and explore their effects at molecular, cellular, pathological, and population levels. Various cooking techniques were considered to mitigate toxin exposure, although complete elimination remains unattainable. Therefore, the findings emphasize the need for cautious consumption. In conclusion, continued research is necessary to better understand and manage its environmental and health implications. Full article

Pteridium aquilinum (L.) Kuhn, commonly known as bracken fern, is a cosmopolitan plant species absent only from polar and desert regions. Worldwide, Pteridium aquilinum has been rapidly spreading due to the neglect of agricultural fields and the growing wave of forest fires. This plant has several toxic components such as thiaminases, brains, active glucosides, beta-glucopyranosides and ptaquloside in its constitution that can be lethal to animals. The animals do not normally choose it as food, except in situations of food shortage, in periods of drought, when the ferns are still green or when the animals graze in places with large amounts of the plant in question. In order to revisit the major syndromes caused by P. aquilinum’s major toxins, the authors present several cases of acute and chronic ruminant poisoning associated with ingesting P. aquilinum in Portugal in sheep and cattle. The primary associated syndromes observed in animals that consume this plant were thiamine deficiency, blindness in sheep, acute or subacute poisoning with bone marrow depression, and consequently, leucopenia and bladder tumors in cattle. Given the economic impact of the diseases caused by the toxins of this plant, and the possible public health implications, it is imperative to minimize these syndromes in animals and establish measures for their prevention. Full article

Hexavalent chromium (Cr VI) is a highly toxic environmental pollutant produced as a byproduct of stainless steel manufacture and leather tanning. Several vegetal Cr VI-accumulating species have been investigated in phytoremediation, a promising technology to remove heavy metals from soils and water bodies. The aim of this work was to test the sensitivity, accumulation and remotion of Cr VI in both life cycle phases of the fern Pteridium aquilinum. Both gametophytes and sporophytes were obtained (in vitro) and evaluated (in vitro and using hydroponics) under controlled temperature, photoperiod and humidity conditions. One-month gametophytes were exposed in vitro to K₂Cr₂O₇ (0, 50, 200, 600 and 800 µM). Four-month sporophytes were exposed to K₂Cr₂O₇ (0, 800, 1600 and 6400 µM) under hydroponic conditions. Both phases were harvested at 24, 28, 72 and 168 h post-exposure, and biomass, chlorophyll content (a and b) and the amount of Cr in tissues and culture medium were tested. The results indicate that both phases of the cycle are not sensitive to Cr VI, since chlorosis and reduction in biomass were not observed. The gametophytes accumulated up to 915 mg of Cr×Kg⁻¹ DW, while the sporophytes accumulated up to 11,854 of Cr×kg⁻¹ DW in the underground parts. The sporophytes showed higher Cr uptake in rhizomes and adventitious roots, and despite having a low translocation index toward the leaves, these reached high concentrations as well (2240 mg Cr×Kg⁻¹ DW). Given the uptake capacity in sporophytes, this fern places within the top five species with highest Cr accumulation, and it may be successfully used in phytoremediation methods. Full article

This study was conducted to determine the optimal boiling time to reduce ptaquiloside (PTA) and to carry out a risk assessment for PTA, a representative toxic substance found in bracken fern (BF; Pteridium aquilinum), which is frequently consumed as food in East Asian countries. High-performance liquid chromatography showed that the concentration of PTA in BF was reduced by up to 99% after boiling for 20 min. Risk assessment results showed that the cancer margin of exposure (MOE; ≥ 25,000 = safe) to PTA for an average daily exposure scenario after boiling BF for 20 min was considered safe. In addition, the non-cancer MOE (≥ 300 = safe) to PTA under an average daily exposure scenario after BF boiling for 20 min was considered safe. However, human exposure to PTA was considered unsafe under the non-boiled BF exposure and maximum daily exposure scenarios. Therefore, boiling BF for at least 20 min is recommended before consumption, to reduce exposure to PTA as much as possible. Full article

Bracken fern (Pteridium aquilium, BF) is a widely consumed vegetable. It has the potential to be manufactured as a ready-to-eat (RTE) product as a cooking ingredient and a side dish. The aim of the current study was to develop sterilized BF RTE products and to investigate textural qualities depending on the size of the pouches. The F₀-value at the cold point according to pouch size (100, 150, and 200 g) targeted at 15 min was determined through heat transfer simulation using the calibrated heat transfer coefficient. The location of the cold points in the stand-up pouches was moved upward from the bottom of the pouch by increasing the pouch size. The sterilization time for 100, 150, and 200 g was evaluated as 35.0, 41.5, and 47.5 min, respectively. The textural properties measured using the cutting test showed significant differences according to the location in the pouch. The textural degradation of BF in the top part of the pouch was more extensive than that at the bottom due to the smaller dimensions. In addition, the percentage of textural degradation in the top part increased with increasing pouch sizes. The methods introduced in this study can be applied to validate the degree of sterilization and the texture of various stalk vegetables used for ready-to-eat products packed in stand-up pouches. Full article

Puffinosis is a disease of a range of seabirds characterised by dorsal and ventral blistering of their webbed feet, conjunctivitis, dry necrosis, leg spasticity, head shaking, loss of balance, tremors, and death. It is associated with Manx shearwaters (Puffinus puffinus), frequently affecting chicks within their underground nesting burrows. The aetiology of the disease is unclear but has been attributed to a type-2 coronavirus associated with Neotombicula mites as a potential vector. However, there is some uncertainty given potential laboratory contamination with mouse hepatitis virus and failure to fulfil Koch’s postulates, with birds injected with isolates remaining healthy. We describe a detailed case report of puffinosis in a Manx Shearwater covering necropsy, histology, bacteriology, and metagenomics including viral sequencing. We found no evidence of viral infection or parasites. Our results are consistent with an entirely environmental aetiology, with caustic faecal ammonia in damp nesting burrows causing conjunctivitis and foot dermatitis breaking the skin, allowing common soil bacteria (i.e., Flavobacterium, Staphylococcus and Serratia spp., Clostridia perfringens and Enterococcus faecalis) to cause opportunistic infection, debilitating the bird and leading to death. A similar condition (foot pad dermatitis or FPD) has been reported in broiler chickens, attributed to caustic faeces, high humidity, and poor environmental conditions during indoor rearing, preventable by adequate ventilation and husbandry. This is consistent with puffinosis being observed in Shearwater nesting burrows situated in tall, dense, vegetation (e.g., bracken Pteridium aquilinum) but rarely reported in burrows situated in well-ventilated, short coastal grasslands. This proposed environmental aetiology accounts for the disease’s non-epizootic prevalence, spatial variation within colonies, and higher frequency in chicks that are restricted to nesting burrows. Full article

Previous studies related to the ptaquiloside molecule, a carcinogenic secondary metabolite known from the world of ferns, are summarised. Ptaquiloside (PTA) belongs to the group of norsesquiterpenes of the illudane type. The name illudane refers to the fungal taxa from which the first representatives of the molecular group were identified. Ptaquiloside occurs mainly in Pteridium fern species, although it is also known in other fern taxa. The species of the genus Pteridium are common, frequent invasive species on all continents, and PTA is formed in smaller or larger amounts in all organs of the affected species. The effects of PTA and of their derivatives on animals and humans are of great toxicological significance. Its basic chemical property is that the molecule can be transformed. First, with the loss of sugar moiety, ptaquilosine is formed, and then, under certain conditions, a dienone derivative (pteridienone) may arise. The latter can alkylate (through its cyclopropane groups) certain molecules, including DNA, in animal or human organisms. In this case, DNA adducts are formed, which can later have a carcinogenic effect through point mutations. The scope of the PTA is interdisciplinary in nature since, for example, molecules from plant biomass can enter the body of animals or humans in several ways (directly and indirectly). Due to its physico-chemical properties (excellent water solubility), PTA can get from the plant into the soil and then into different water layers. PTA molecules that enter the soil, but mainly water, undergo degradation (hydrolytic) processes, so it is very important to clarify the toxicological conditions of a given ecosystem and to estimate the possible risks caused by PTA. The toxicoses and diseases of the animal world (mainly for ruminant farm animals) caused by PTA are briefly described. The intake of PTA-containing plants as a feed source causes not only various syndromes but can also enter the milk (and meat) of animals. In connection with the toxicological safety of the food chain, it is important to investigate the transport of carcinogenic PTA metabolites between organisms in a reassuring manner and in detail. This is a global, interdisciplinary task. The present review aims to contribute to this. Full article

Plant toxicology has affected animals throughout evolution. Plants have adapted themselves to the environment. This adaptation has led to the development of defensive strategies to avoid being consumed. Plants have several chemical compounds, which can cause deleterious effects on people or animals that consume them, causing a wide variety of clinical signs. Plants from various latitudes, both cultivated for human and animal feeding or decorative purpose and even wild growth plants are able to generate anaemia in ruminants. Coumarins or ptaquiloside predispose bleeding and haemorrhages, causing a haemorrhagic disease in affected animals. In this group, some important fodder plants, such sweet clover (Genus Melilotus spp.), or other weeds distributed worldwide, such as bracken fern (Pteridium aquilinum) of giant fennel (Ferula communis), are included. On the other hand, sulfur-containing chemicals (e.g., n-propyl disulfate and S-propyl cysteine sulfoxides (SMCOs)) may cause severe direct damage to the erythrocyte and their membrane, leading to their destruction and causing haemolytic anaemia in the animal. This review presents the most frequent intoxication by plants causing anaemia in ruminants. Toxic compounds, clinical signs, diagnosis and possible treatments are also presented. Full article

Pteridium aquilinum (L.) Kuhn (Pteridaceae family) has been widely used as a food and medicine in China and Korea. Previous studies indicate that P. aquilinum contains a variety of bioactive chemical components such as flavonoids, phenols, terpenoids, saponins, polysaccharides, and so on. In the present study, a novel polysaccharide (named as PAP-3) with average molecular weight of 2.14 × 10⁵ Da was obtained from P. aquilinum. The structure was studied through physicochemical and spectroscopic analysis. The results indicated that PAP-3 consists of arabinose, rhamnose, fucose, galactose, mannose, and xylose in a molar ratio of 1.58:1.00:3.26:4.57:4.81:3.33. The polysaccharide is mainly composed of (1→2)-linked xylose and (1→3,6)-linked mannose on the main chain, with (1→2)-linked xylose, (1→6)-linked mannose, and (1→6)- and (1→3,6)-linked galactose as side chains. Galactose, fucose, and xylose are located at the end of the side chains. The in vitro immunomodulatory and antioxidant activities were assayed. PAP-3 has strong free-radical scavenging activity on DPPH and ABTS radicals and significant immunomodulatory activity on RAW264.7 cells. These data provide useful information for further study on the polysaccharides of P. aquilinum and their applications in the food and medical industries. Full article