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11 pages, 638 KiB  
Communication
Millet in Bioregenerative Life Support Systems: Hypergravity Resilience and Predictive Yield Models
by Tatiana S. Aniskina, Arkady N. Kudritsky, Olga A. Shchuklina, Nikita E. Andreev and Ekaterina N. Baranova
Life 2025, 15(8), 1261; https://doi.org/10.3390/life15081261 (registering DOI) - 7 Aug 2025
Abstract
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using [...] Read more.
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using millet in biological life support systems and to create predictive models of yield components for automating plant cultivation control. The study found that stress from hypergravity (800 g, 1200 g, 2000 g, and 3000 g) in the early stages of millet germination does not affect seedlings or yield. In a closed system, millet yield reached 0.31 kg/m2, the weight of 1000 seeds was 8.61 g, and the yield index was 0.06. The paper describes 40 quantitative traits, including six leaf and trichome traits and nine grain traits from the lower, middle and upper parts of the inflorescence. The compiled predictive regression equations allow predicting the accumulation of biomass in seedlings on the 10th and 20th days of cultivation, as well as the weight of 1000 seeds, the number of productive inflorescences, the total above-ground mass, and the number and weight of grains per plant. These equations open up opportunities for the development of computer vision and high-speed plant phenotyping programs that will allow automatic correction of the plant cultivation process and modeling of the required yield. Predicting biomass yield will also be useful in assessing the load on the waste-free processing system for plant waste at planetary stations. Full article
(This article belongs to the Special Issue Physiological Responses of Plants Under Abiotic Stresses)
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19 pages, 3601 KiB  
Article
Study on Correction Methods for GPM Rainfall Rate and Radar Reflectivity Using Ground-Based Raindrop Spectrometer Data
by Lin Chen, Huige Di, Dongdong Chen, Ning Chen, Qinze Chen and Dengxin Hua
Remote Sens. 2025, 17(15), 2747; https://doi.org/10.3390/rs17152747 (registering DOI) - 7 Aug 2025
Abstract
The Dual-frequency Precipitation Radar (DPR) aboard the Global Precipitation Measurement (GPM) mission provides valuable three-dimensional precipitation structure data on a global scale and has been widely used in hydrometeorological research. However, due to its spatial resolution limitations and inherent algorithmic assumptions, the accuracy [...] Read more.
The Dual-frequency Precipitation Radar (DPR) aboard the Global Precipitation Measurement (GPM) mission provides valuable three-dimensional precipitation structure data on a global scale and has been widely used in hydrometeorological research. However, due to its spatial resolution limitations and inherent algorithmic assumptions, the accuracy of GPM precipitation estimates can exhibit systematic biases, especially under complex terrain conditions or in the presence of variable precipitation structures, such as light stratiform rain or intense convective storms. In this study, we evaluated the near-surface precipitation rate estimates from the GPM-DPR Level 2A product using over 1440 min of disdrometer observations collected across China from 2021 to 2023. Based on three years of stable stratiform precipitation data from the Jinghe station, we developed a least squares linear correction model for radar reflectivity. Independent validation using national disdrometer data from 2023 demonstrated that the corrected reflectivity significantly improved rainfall estimates under light precipitation conditions, although improvements were limited for convective events or in complex terrain. To further enhance retrieval accuracy, we introduced a regionally adaptive R–Z relationship scheme stratified by precipitation type and terrain category. Applying these localized relationships to the corrected reflectivity yielded more consistent rainfall estimates across diverse conditions, highlighting the importance of incorporating regional microphysical characteristics into satellite retrieval algorithms. The results indicate that the accuracy of GPM precipitation retrievals is more significantly influenced by precipitation type than by terrain complexity. Under stratiform precipitation conditions, the GPM-estimated precipitation data demonstrate the highest reliability. The correction framework proposed in this study is grounded on ground-based observations and integrates regional precipitation types with terrain characteristics. It effectively enhances the applicability of GPM-DPR products across diverse environmental conditions in China and offers a methodological reference for correcting satellite precipitation biases in other regions. Full article
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14 pages, 74879 KiB  
Article
Upscaling In Situ and Airborne Hyperspectral Data for Satellite-Based Chlorophyll Retrieval in Coastal Waters
by Roko Andričević
Water 2025, 17(15), 2356; https://doi.org/10.3390/w17152356 (registering DOI) - 7 Aug 2025
Abstract
Monitoring water quality parameters in coastal and estuarine environments is critical for assessing their ecological status and addressing environmental challenges. However, traditional in situ sampling programs are often constrained by limited spatial and temporal coverage, making it difficult to capture the complex variability [...] Read more.
Monitoring water quality parameters in coastal and estuarine environments is critical for assessing their ecological status and addressing environmental challenges. However, traditional in situ sampling programs are often constrained by limited spatial and temporal coverage, making it difficult to capture the complex variability in these dynamic systems. This study introduces a novel upscaling framework that leverages limited in situ measurements and airborne hyperspectral data to generate multiple conditional realizations of water quality parameter fields. These pseudo-measurements are statistically consistent with the original data and are used to calibrate inversion algorithms that relate satellite-derived reflectance data to water quality parameters. The approach was applied to Kaštela Bay, a semi-enclosed coastal area in the eastern Adriatic Sea, to map seasonal variations in water quality parameters such as Chlorophyll-a. The upscaling framework captured spatial patterns that were absent in sparse in situ observations and enabled regional mapping using Sentinel-2A satellite data at the appropriate spatial scale. By generating realistic pseudo-measurements, the method improved the stability and performance of satellite-based retrieval algorithms, particularly in periods of high productivity. Overall, this methodology addresses data scarcity challenges in coastal water monitoring and its application could benefit the implementation of European water quality directives through enhanced regional-scale mapping capabilities. Full article
(This article belongs to the Section Oceans and Coastal Zones)
17 pages, 4238 KiB  
Article
Carbonatogenic Bacteria from Corallium rubrum Colonies
by Vincenzo Pasquale, Roberto Sandulli, Elena Chianese, Antonio Lettino, Maria Esther Sanz-Montero, Mazhar Ali Jarwar and Stefano Dumontet
Minerals 2025, 15(8), 839; https://doi.org/10.3390/min15080839 (registering DOI) - 7 Aug 2025
Abstract
The precipitation of minerals, in particular carbonates, is a widespread phenomenon in all ecosystems, where it assumes a high relevance both from a geological and biogeochemical standpoint. Most carbonate rocks are of biological origin and made in an aquatic environment. In particular, bioprecipitation [...] Read more.
The precipitation of minerals, in particular carbonates, is a widespread phenomenon in all ecosystems, where it assumes a high relevance both from a geological and biogeochemical standpoint. Most carbonate rocks are of biological origin and made in an aquatic environment. In particular, bioprecipitation of carbonates is believed to have started in the Mesoproterozoic Era, thanks to a process often driven by photosynthetic microorganisms. Nevertheless, an important contribution to carbonate precipitation is also due to the metabolic activity of heterotrophic bacteria, which is not restricted to specific taxonomic groups or to specific environments, making this process a ubiquitous phenomenon. In this framework, the relationship between carbonatogenic microorganisms and other living organisms assumes a particular interest. This study aims to isolate and identify the culturable heterotrophic bacterial component associated with the coenosarc of Corallium rubrum in order to evaluate the occurrence of strains able to precipitate carbonates. In particular, the study was focused on the identification and characterisation of bacterial strains isolated from a coral coenosarc showing a high carbonatogenic capacity under laboratory conditions. Samples of C. rubrum were taken in the coastal waters of three Italian regions. The concentration of the aerobic heterotrophic microflora colonising C. rubrum coenosarc samples spanned from 3 to 6∙106 CFU/cm2. This variation in microbial populations colonising the C. rubrum coenosarc, spanning over 6 orders of magnitude, is not mirrored by a corresponding variability in the colony morphotypes recorded, with the mean being 5.1 (±2.1 sd). Among these bacteria, the carbonatogenic predominant species was Staphylococcus equorum (93% of the isolates), whereas Staphylococcus xylosus and Shewanella sp. accounted only for 3% of isolates each. All these strains showed a remarkable capacity of precipitating calcium carbonate, in the form of calcite crystals organised radially as well crystalised spherulites (S. equorum) or coalescing spherulites (Shewanella sp.). S. xylosus only produced amorphous precipitates of calcium carbonate. All bacterial strains identified were positive both for the production of urease and carbon anhydrase in vitro at 30 °C. It seems that they potentially possess the major biochemical abilities conducive to Ca2+ precipitation, as they showed in vitro. In addition, all our carbonatogenic isolates were able to hydrolyse the phytic acid calcium salt and then were potentially able to induce precipitation of calcium phosphates also through such a mechanism. Full article
(This article belongs to the Special Issue Carbonate Petrology and Geochemistry, 2nd Edition)
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19 pages, 4005 KiB  
Article
Analysis of Temporal and Spatial Variations in Cropland Water-Use Efficiency and Influencing Factors in Xinjiang Based on the XGBoost–SHAP Model
by Qiu Zhao, Fan Gao, Bing He, Ying Li, Hairui Li, Yao Xiao and Ruzhang Lin
Agronomy 2025, 15(8), 1902; https://doi.org/10.3390/agronomy15081902 (registering DOI) - 7 Aug 2025
Abstract
In arid regions with limited water resources, improving cropland water-use efficiency (WUEc) is crucial for maintaining crop production. This study aims to investigate how changes in meteorological and vegetation factors affect WUEc in drylands and to identify its primary drivers, which are essential [...] Read more.
In arid regions with limited water resources, improving cropland water-use efficiency (WUEc) is crucial for maintaining crop production. This study aims to investigate how changes in meteorological and vegetation factors affect WUEc in drylands and to identify its primary drivers, which are essential for understanding how cropland ecosystems respond to complex environmental changes. Using remote sensing data, we analyzed the spatiotemporal patterns of WUEc in Xinjiang from 2002 to 2022 by applying STL decomposition, Sen’s slope combined with the Mann–Kendall test, and an XGBoost–SHAP model, quantifying its key controlling factors. The results indicate that from 2002 to 2022, WUEc in Xinjiang showed an overall declining trend. Prior to 2007, WUEc increased at 0.05 gC·m−1·m−2·a−1, after which it fluctuated downward at −0.01 gC·m−1·m−2·a−1. Intra-annual peaks consistently occurred in May and during September–October. Spatially, WUEc exhibited significant heterogeneity, increasing from south to north, with 53.26% of the region showing declines. Temperature (T) and leaf area index (LAI) emerged as the primary meteorological and vegetation drivers, respectively, influencing WUEc change in 45.7% and 17.6% of the area. Both variables were negatively correlated with WUEc, with negative correlations covering 60% of the region for T and 83% for LAI. These findings provide scientific guidance for optimizing crop structure and water-resource management strategies in arid regions. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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18 pages, 2405 KiB  
Article
Dynamic Comparative Assessment of Long-Term Simulation Strategies for an Off-Grid PV–AEM Electrolyzer System
by Roberta Caponi, Domenico Vizza, Claudia Bassano, Luca Del Zotto and Enrico Bocci
Energies 2025, 18(15), 4209; https://doi.org/10.3390/en18154209 (registering DOI) - 7 Aug 2025
Abstract
Among the various renewable-powered pathways for green hydrogen production, solar photovoltaic (PV) technology represents a particularly promising option due to its environmental sustainability, widespread availability, and declining costs. However, the inherent intermittency of solar irradiance presents operational challenges for electrolyzers, particularly in terms [...] Read more.
Among the various renewable-powered pathways for green hydrogen production, solar photovoltaic (PV) technology represents a particularly promising option due to its environmental sustainability, widespread availability, and declining costs. However, the inherent intermittency of solar irradiance presents operational challenges for electrolyzers, particularly in terms of stability and efficiency. This study presents a MATLAB-based dynamic model of an off-grid, DC-coupled solar PV-Anion Exchange Membrane (AEM) electrolyzer system, with a specific focus on realistically estimating hydrogen output. The model incorporates thermal energy management strategies, including electrolyte pre-heating during startup, and accounts for performance degradation due to load cycling. The model is designed for a comprehensive analysis of hydrogen production by employing a 10-year time series of irradiance and ambient temperature profiles as inputs. The results are compared with two simplified scenarios: one that does not consider the equipment response time to variable supply and another that assumes a fixed start temperature to evaluate their impact on productivity. Furthermore, to limit the effects of degradation, the algorithm has been modified to allow the non-sequential activation of the stacks, resulting in an improvement of the single stack efficiency over the lifetime and a slight increase in overall hydrogen production. Full article
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22 pages, 541 KiB  
Article
Patent Licensing Strategy for Supply Chain Reshaping Under Sudden Disruptive Events
by Jianxin Zhu, Xinying Wang, Nengmin Zeng and Huijian Zhong
Systems 2025, 13(8), 672; https://doi.org/10.3390/systems13080672 (registering DOI) - 7 Aug 2025
Abstract
Supply chains are increasingly exposed to sudden disruptive events (SDEs) such as natural disasters and trade wars. We develop a multi-stage game-theoretical model to investigate a novel coping mechanism: when a firm is forced to exit the market because of SDEs, the firm [...] Read more.
Supply chains are increasingly exposed to sudden disruptive events (SDEs) such as natural disasters and trade wars. We develop a multi-stage game-theoretical model to investigate a novel coping mechanism: when a firm is forced to exit the market because of SDEs, the firm can regain profits by licensing its proprietary production tech to a competitor. We find that, compared with the scenario before SDEs, such events can even increase the profit of each manufacturer under certain conditions. Under certain conditions, the cooperative strategy (i.e., supply chain reshaping) yields a higher supply chain system profit than the non-cooperative strategy. After SDEs, the common manufacturer may either accept or reject cooperation, depending on the customer transfer rate and the cooperation cost. Notably, under the cooperation strategy, the high-tech manufacturer extracts part of the common manufacturer’s profit through patent licensing, and the existence of cooperation cost further contributes to a misalignment between the common manufacturer’s optimal decision and the supply chain system optimum. These findings contribute to the literature by identifying a novel supply chain reshaping mechanism driven by patent licensing and offer strategic guidance for firms and policymakers navigating SDE-induced market exits. Full article
(This article belongs to the Special Issue Operation and Supply Chain Risk Management)
24 pages, 4458 KiB  
Review
Selenium-Enriched Microorganisms: Metabolism, Production, and Applications
by Lin Luo, Xue Hou, Dandan Yi, Guangai Deng, Zhiyong Wang and Mu Peng
Microorganisms 2025, 13(8), 1849; https://doi.org/10.3390/microorganisms13081849 (registering DOI) - 7 Aug 2025
Abstract
Microorganisms, as abundant biological resources, offer significant potential in the development of selenium-enrichment technologies. Selenium-enriched microorganisms not only absorb, reduce, and accumulate selenium efficiently but also produce various selenium compounds without relying on synthetic chemical processes. In particular, nano-selenium produced by these microorganisms [...] Read more.
Microorganisms, as abundant biological resources, offer significant potential in the development of selenium-enrichment technologies. Selenium-enriched microorganisms not only absorb, reduce, and accumulate selenium efficiently but also produce various selenium compounds without relying on synthetic chemical processes. In particular, nano-selenium produced by these microorganisms during cultivation has garnered attention due to its unique physicochemical properties and biological activity, making it a promising raw material for functional foods and pharmaceutical products. This paper reviews selenium-enriched microorganisms, focusing on their classification, selenium metabolism, and transformation mechanisms. It explores how selenium is absorbed, reduced, and transformed within microbial cells, analyzing the biochemical processes by which inorganic selenium is converted into organic and nano-selenium forms. Finally, the broad applications of selenium-enriched microbial products in food, medicine, and agriculture are explored, including their roles in selenium-rich foods, nano-selenium materials, and disease prevention and treatment. Full article
(This article belongs to the Special Issue Exploring the Diversity of Microbial Applications)
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22 pages, 681 KiB  
Article
Unlocking the Nexus: Personal Remittances and Economic Drivers Shaping Housing Prices Across EU Borders
by Maja Nikšić Radić, Siniša Bogdan and Marina Barkiđija Sotošek
World 2025, 6(3), 112; https://doi.org/10.3390/world6030112 (registering DOI) - 7 Aug 2025
Abstract
This study examines the impact of personal remittances on housing prices in European Union (EU) countries, while also accounting for a broader set of macroeconomic, demographic, and structural variables. Using annual data for 27 EU countries from 2007 to 2022, we employ a [...] Read more.
This study examines the impact of personal remittances on housing prices in European Union (EU) countries, while also accounting for a broader set of macroeconomic, demographic, and structural variables. Using annual data for 27 EU countries from 2007 to 2022, we employ a comprehensive panel econometric approach, including cross-sectional dependence tests, second-generation unit root tests, pooled mean group–autoregressive distributed lag (PMG-ARDL) estimation, and panel causality tests, to capture both short- and long-term dynamics. Our findings confirm that remittances significantly and positively influence long-term housing price levels, underscoring their relevance as a demand-side driver. Other key variables such as net migration, GDP, travel credit to GDP, economic freedom, and real effective exchange rates also contribute to housing price movements, while supply-side indicators, including production in construction and building permits, exert moderating effects. Moreover, real interest rates are shown to have a significant long-term negative effect on property prices. The analysis reveals key causal links from remittances, FDI, and net migration to housing prices, highlighting their structural and predictive roles. Bidirectional causality between economic freedom, housing output, and prices indicates reinforcing feedback effects. These findings position remittances as both a development tool and a key indicator of real estate dynamics. The study highlights complex interactions between international financial flows, demographic pressures, and domestic economic conditions and the need for policymakers to consider remittances and migrant investments in real estate strategies. These findings offer important implications for policymakers seeking to balance housing affordability, investment, and economic resilience in the EU context and key insights into the complexity of economic factors and real estate prices. Importantly, the analysis identifies several causal relationships, notably from remittances, FDI, and net migration toward housing prices, underscoring their predictive and structural importance. Bidirectional causality between economic freedom and house prices, as well as between housing output and pricing, reflects feedback mechanisms that further reinforce market dynamics. These results position remittances not only as a developmental instrument but also as a key signal for real estate market performance in recipient economies. Full article
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14 pages, 706 KiB  
Article
Study on the Effects of Irrigation Amount on Spring Maize Yield and Water Use Efficiency Under Different Planting Patterns in Xinjiang
by Ruxiao Bai, Haixiu He, Xinjiang Zhang and Qifeng Wu
Agriculture 2025, 15(15), 1710; https://doi.org/10.3390/agriculture15151710 (registering DOI) - 7 Aug 2025
Abstract
Planting patterns and irrigation amounts are key factors affecting maize yield. This study adopted a two-factor experimental design, with planting pattern as the main plot and irrigation amount as the subplot, to investigate the effects of irrigation levels under different planting patterns (including [...] Read more.
Planting patterns and irrigation amounts are key factors affecting maize yield. This study adopted a two-factor experimental design, with planting pattern as the main plot and irrigation amount as the subplot, to investigate the effects of irrigation levels under different planting patterns (including uniform row spacing and alternating wide-narrow row spacing) on spring maize yield and water use efficiency in Xinjiang. Through this approach, the study examined the mechanisms by which planting pattern and irrigation amount influence maize growth, yield formation, and water use efficiency. Experiments conducted at the Agricultural Science Research Institute of the Ninth Division of Xinjiang Production and Construction Corps demonstrated that alternating wide-narrow row spacing combined with moderate irrigation (5400 m3/hm2) significantly optimized maize root distribution, improved water use efficiency, and increased leaf area index and net photosynthetic rate, thereby promoting dry matter accumulation and yield enhancement. In contrast, uniform row spacing under high irrigation levels increased yield but resulted in lower water use efficiency. The study also found that alternating wide-narrow row spacing enhanced maize nutrient absorption from the soil, particularly phosphorus utilization efficiency, by improving canopy structure and root expansion. This pattern exhibited comprehensive advantages in resource utilization, providing a theoretical basis and technical pathway for achieving water-saving and high-yield maize production in arid regions, which holds significant importance for promoting sustainable agricultural development. Full article
(This article belongs to the Section Crop Production)
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21 pages, 1609 KiB  
Article
Exploring Residual Clays for Low-Impact Ceramics: Insights from a Portuguese Ceramic Region
by Carla Candeias, Sónia Novo and Fernando Rocha
Appl. Sci. 2025, 15(15), 8761; https://doi.org/10.3390/app15158761 (registering DOI) - 7 Aug 2025
Abstract
This study investigates the potential of residual clays from a traditional ceramic-producing region in southern Portugal as raw materials for red ceramic applications. This work aims to support more sustainable ceramic practices through the local valorization of naturally available, underutilized clay resources. A [...] Read more.
This study investigates the potential of residual clays from a traditional ceramic-producing region in southern Portugal as raw materials for red ceramic applications. This work aims to support more sustainable ceramic practices through the local valorization of naturally available, underutilized clay resources. A multidisciplinary approach was employed to characterize clays, integrating mineralogical (XRD), chemical (XRF), granulometric, and thermal analyses (TGA/DTA/TD), as well as technological tests on plasticity, extrusion moisture, shrinkage, and flexural strength. These assessments were designed to capture both the intrinsic properties of the clays and their behavior across key ceramic processing stages, such as shaping, drying, and firing. The results revealed a broad diversity in mineral composition, particularly in the proportions of kaolinite, smectite, and illite, which strongly influenced plasticity, water demand, and thermal stability. Clays with higher fine fractions and smectitic content exhibited excellent plasticity and workability, though with increased sensitivity to drying and firing conditions. Others, with coarser textures and illitic or feldspathic composition, demonstrated improved dimensional stability and lower shrinkage. Thermal analyses confirmed expected dehydroxylation and sintering behavior, with the formation of mullite and spinel-type phases contributing to densification and strength in fired bodies. This study highlights that residual clays from varied geological settings can offer distinct advantages when matched appropriately to ceramic product requirements. Some materials showed strong potential for direct application in structural ceramics, while others may serve as additives or tempering agents in formulations. These findings reinforce the value of integrated characterization for optimizing raw material use and support a more circular, resource-conscious approach to ceramic production. Full article
25 pages, 7934 KiB  
Article
An Improved InTEC Model for Estimating the Carbon Budgets in Eucalyptus Plantations
by Zhipeng Li, Mingxing Zhou, Kunfa Luo, Yunzhong Wu and Dengqiu Li
Remote Sens. 2025, 17(15), 2741; https://doi.org/10.3390/rs17152741 (registering DOI) - 7 Aug 2025
Abstract
Eucalyptus has become a major plantation crop in southern China, with a carbon sequestration capacity significantly higher than that of other species. However, its long-term carbon sequestration capacity and regional-scale potential remain highly uncertain due to commonly applied short-rotation management practices. The InTEC [...] Read more.
Eucalyptus has become a major plantation crop in southern China, with a carbon sequestration capacity significantly higher than that of other species. However, its long-term carbon sequestration capacity and regional-scale potential remain highly uncertain due to commonly applied short-rotation management practices. The InTEC (Integrated Terrestrial Ecosystem Carbon) model is a process-based biogeochemical model that simulates carbon dynamics in terrestrial ecosystems by integrating physiological processes, environmental drivers, and management practices. In this study, the InTEC model was enhanced with an optimized eucalyptus module (InTECeuc) and a data assimilation module (InTECDA), and driven by multiple remote sensing products (Net Primary Productivity (NPP) and carbon density) to simulate the carbon budgets of eucalyptus plantations from 2003 to 2023. The results indicated notable improvements in the performance of the InTECeuc model when driven by different datasets: carbon density simulation showed improvements in R2 (0.07–0.56), reductions in MAE (5.99–28.51 Mg C ha−1), reductions in RMSE (8.1–31.85 Mg C ha−1), and improvements in rRMSE (12.37–51.82%), excluding NPPLin. The carbon density-driven InTECeuc model outperformed the NPP-driven model, with improvements in R2 (0.28), MAE (−8.15 Mg C ha−1), RMSE (−9.43 Mg C ha−1), and rRMSE (−15.34%). When the InTECDA model was employed, R2 values for carbon density improved by 0–0.03 (excluding ACDYan), with MAE reductions between 0.17 and 7.22 Mg C ha−1, RMSE reductions between 0.33 and 12.94 Mg C ha−1 and rRMSE improvements ranging from 0.51 to 20.22%. The carbon density-driven InTECDA model enabled the production of high-resolution and accurate carbon budget estimates for eucalyptus plantations from 2003 to 2023, with average NPP, Net Ecosystem Productivity (NEP), and Net Biome Productivity (NBP) values of 17.80, 10.09, and 9.32 Mg C ha−1 yr−1, respectively, offering scientific insights and technical support for the management of eucalyptus plantations in alignment with carbon neutrality targets. Full article
10 pages, 523 KiB  
Article
Mutation Rates and Fitness Genes in Staphylococcus aureus Treated with the Medicinal Plant Synadenium glaucescens
by Zaituni Msengwa, Martin Saxtorph Bojer, Frank Rwegoshora, James Mwesongo, Magesa Mafuru, Faith Philemon Mabiki, Beda John Mwang’onde, Madundo Mkumbukwa Mtambo, Lughano Jeremy Kusiluka, Henrik Christensen, Robinson Hammerthon Mdegela and John Elmerdahl Olsen
Appl. Sci. 2025, 15(15), 8753; https://doi.org/10.3390/app15158753 (registering DOI) - 7 Aug 2025
Abstract
Extracts, fractions and the pure compound epifriedelanol of the medicinal plant Synadenium glaucescens have antibacterial properties. Herbal products are generally considered less prone to resistance development than conventional antimicrobials, as they contain multiple compounds, which makes bacteria less likely to develop resistance. However, [...] Read more.
Extracts, fractions and the pure compound epifriedelanol of the medicinal plant Synadenium glaucescens have antibacterial properties. Herbal products are generally considered less prone to resistance development than conventional antimicrobials, as they contain multiple compounds, which makes bacteria less likely to develop resistance. However, data supporting this notion are lacking. This study evaluated the development of resistance in Staphylococcus aureus subjected to extract, fractions and epifriedelanol of S. glaucescens. It also identified S. aureus fitness genes contributing to intrinsic resistance to extract of S. glaucescens. Fluctuation and gradient concentration assays were used to determine mutation rates and growth adaptation, respectively, which were lower following exposure to growth in crude extract than the pure compound epifriedelanol. By subjecting 1920 single gene mutants from the Nebraska Transposon Mutant Library to growth in the presence of extract of S. glaucescens, 12 genes were identified as important for natural resistance in S. aureus JE2; however, only mutation in the hemB gene decreased the minimum inhibitory concentration by greater than 4-fold (64-fold). In conclusion, purifying active antimicrobial compounds from S. glaucescens and using them as antibacterial substances as an alternative to crude extract increased the risk of resistance development. Further, the gene hemBappears to have a significant role in the natural resistance to the extracts obtained from S. glaucescens in this study. Full article
15 pages, 3707 KiB  
Article
Biodegradation of Both Ethanol and Acetaldehyde by Acetobacter ghanensis JN01
by Hongyan Liu, Jingjing Wang, Qianqian Xu, Xiaoyu Cao, Xinyue Du, Kun Lin and Hai Yan
Catalysts 2025, 15(8), 756; https://doi.org/10.3390/catal15080756 (registering DOI) - 7 Aug 2025
Abstract
Excessive alcohol consumption is associated with systemic health risks due to the production of acetaldehyde, a primary carcinogen that not only pollutes the environment but also endangers human health. In this study, a promising bacterial strain for biodegrading both ethanol and acetaldehyde was [...] Read more.
Excessive alcohol consumption is associated with systemic health risks due to the production of acetaldehyde, a primary carcinogen that not only pollutes the environment but also endangers human health. In this study, a promising bacterial strain for biodegrading both ethanol and acetaldehyde was successfully isolated from the traditional fermented food Jiaosu and identified as Acetobacter ghanensis JN01 based on average nucleotide identity (ANI) analysis. Initial ethanol of 1 g/L was completely biodegraded within 4 h, while initial acetaldehyde of 1 g/L was also rapidly removed at 2 or 1 h by whole cells or cell-free extracts (CEs) of JN01, respectively, which indicated that JN01 indeed has a strong ability in the biodegradation of both ethanol and acetaldehyde. Whole-genome sequencing revealed a 2.85 Mb draft genome of JN01 with 57.0% guanine–cytosine (GC) content and the key metabolic genes (adh1, adh2, and aldh) encoding involving alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), co-located with NADH dehydrogenase genes and ethanol-responsive regulatory motifs, supporting the metabolic pathway of transforming ethanol to acetaldehyde, and, subsequently, converting acetaldehyde to acetic acid. Furthermore, selected in vitro safety-related traits of JN01 were also assessed, which is very important in the development of microbial catalysts against both ethanol and acetaldehyde. Full article
(This article belongs to the Section Biocatalysis)
24 pages, 2812 KiB  
Article
Application of a Multi-Algorithm-Optimized CatBoost Model in Predicting the Strength of Multi-Source Solid Waste Backfilling Materials
by Jianhui Qiu, Jielin Li, Xin Xiong and Keping Zhou
Big Data Cogn. Comput. 2025, 9(8), 203; https://doi.org/10.3390/bdcc9080203 (registering DOI) - 7 Aug 2025
Abstract
Backfilling materials are commonly employed materials in mines for filling mining waste, and the strength of the consolidated backfill formed by the binding material directly influences the stability of the surrounding rock and production safety in mines. The traditional approach to obtaining the [...] Read more.
Backfilling materials are commonly employed materials in mines for filling mining waste, and the strength of the consolidated backfill formed by the binding material directly influences the stability of the surrounding rock and production safety in mines. The traditional approach to obtaining the strength of the backfill demands a considerable amount of manpower and time. The rapid and precise acquisition and optimization of backfill strength parameters hold utmost significance for mining safety. In this research, the authors carried out a backfill strength experiment with five experimental parameters, namely concentration, cement–sand ratio, waste rock–tailing ratio, curing time, and curing temperature, using an orthogonal design. They collected 174 sets of backfill strength parameters and employed six population optimization algorithms, including the Artificial Ecosystem-based Optimization (AEO) algorithm, Aquila Optimization (AO) algorithm, Germinal Center Optimization (GCO), Sand Cat Swarm Optimization (SCSO), Sparrow Search Algorithm (SSA), and Walrus Optimization Algorithm (WaOA), in combination with the CatBoost algorithm to conduct a prediction study of backfill strength. The study also utilized the Shapley Additive explanatory (SHAP) method to analyze the influence of different parameters on the prediction of backfill strength. The results demonstrate that when the population size was 60, the AEO-CatBoost algorithm model exhibited a favorable fitting effect (R2 = 0.947, VAF = 93.614), and the prediction error was minimal (RMSE = 0.606, MAE = 0.465), enabling the accurate and rapid prediction of the strength parameters of the backfill under different ratios and curing conditions. Additionally, an increase in curing temperature and curing time enhanced the strength of the backfill, and the influence of the waste rock–tailing ratio on the strength of the backfill was negative at a curing temperature of 50 °C, which is attributed to the change in the pore structure at the microscopic level leading to macroscopic mechanical alterations. When the curing conditions are adequate and the parameter ratios are reasonable, the smaller the porosity rate in the backfill, the greater the backfill strength will be. This study offers a reliable and accurate method for the rapid acquisition of backfill strength and provides new technical support for the development of filling mining technology. Full article
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