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Article

Biodegradation of Both Ethanol and Acetaldehyde by Acetobacter ghanensis JN01

School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Catalysts 2025, 15(8), 756; https://doi.org/10.3390/catal15080756 (registering DOI)
Submission received: 3 July 2025 / Revised: 3 August 2025 / Accepted: 5 August 2025 / Published: 7 August 2025
(This article belongs to the Section Biocatalysis)

Abstract

Excessive alcohol consumption is associated with systemic health risks due to the production of acetaldehyde, a primary carcinogen that not only pollutes the environment but also endangers human health. In this study, a promising bacterial strain for biodegrading both ethanol and acetaldehyde was successfully isolated from the traditional fermented food Jiaosu and identified as Acetobacter ghanensis JN01 based on average nucleotide identity (ANI) analysis. Initial ethanol of 1 g/L was completely biodegraded within 4 h, while initial acetaldehyde of 1 g/L was also rapidly removed at 2 or 1 h by whole cells or cell-free extracts (CEs) of JN01, respectively, which indicated that JN01 indeed has a strong ability in the biodegradation of both ethanol and acetaldehyde. Whole-genome sequencing revealed a 2.85 Mb draft genome of JN01 with 57.0% guanine–cytosine (GC) content and the key metabolic genes (adh1, adh2, and aldh) encoding involving alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), co-located with NADH dehydrogenase genes and ethanol-responsive regulatory motifs, supporting the metabolic pathway of transforming ethanol to acetaldehyde, and, subsequently, converting acetaldehyde to acetic acid. Furthermore, selected in vitro safety-related traits of JN01 were also assessed, which is very important in the development of microbial catalysts against both ethanol and acetaldehyde.
Keywords: Acetobacter ghanensis JN01; ethanol; acetaldehyde; biodegradation; genome analysis Acetobacter ghanensis JN01; ethanol; acetaldehyde; biodegradation; genome analysis

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MDPI and ACS Style

Liu, H.; Wang, J.; Xu, Q.; Cao, X.; Du, X.; Lin, K.; Yan, H. Biodegradation of Both Ethanol and Acetaldehyde by Acetobacter ghanensis JN01. Catalysts 2025, 15, 756. https://doi.org/10.3390/catal15080756

AMA Style

Liu H, Wang J, Xu Q, Cao X, Du X, Lin K, Yan H. Biodegradation of Both Ethanol and Acetaldehyde by Acetobacter ghanensis JN01. Catalysts. 2025; 15(8):756. https://doi.org/10.3390/catal15080756

Chicago/Turabian Style

Liu, Hongyan, Jingjing Wang, Qianqian Xu, Xiaoyu Cao, Xinyue Du, Kun Lin, and Hai Yan. 2025. "Biodegradation of Both Ethanol and Acetaldehyde by Acetobacter ghanensis JN01" Catalysts 15, no. 8: 756. https://doi.org/10.3390/catal15080756

APA Style

Liu, H., Wang, J., Xu, Q., Cao, X., Du, X., Lin, K., & Yan, H. (2025). Biodegradation of Both Ethanol and Acetaldehyde by Acetobacter ghanensis JN01. Catalysts, 15(8), 756. https://doi.org/10.3390/catal15080756

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