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23 pages, 2122 KB  
Article
Pesticide Residues in Pome Fruits: Occurrence, Quality Profiling, and Advanced Dietary Risk Characterisation
by Nimo Hussein Yussuf, Tuba Buyuksirit-Bedir, Cagla Kayisoglu, Eylem Odabas, Fatma Oznur Afacan, Ozgur Golge, Tamara Lazarević-Pašti and Bulent Kabak
Molecules 2026, 31(12), 2132; https://doi.org/10.3390/molecules31122132 - 17 Jun 2026
Viewed by 323
Abstract
The occurrence of pesticide residues in pome fruits and their implications for consumer health remain critical concerns in food safety. In this study, 222 pesticide residues were analysed in 155 samples of apples, pears, and quinces collected from Türkiye between October 2025 and [...] Read more.
The occurrence of pesticide residues in pome fruits and their implications for consumer health remain critical concerns in food safety. In this study, 222 pesticide residues were analysed in 155 samples of apples, pears, and quinces collected from Türkiye between October 2025 and March 2026 using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Residues were detected in 76.4% of apples, 86% of pears, and 30% of quinces, with frequent multi-residue patterns and notable occurrences of non-approved compounds. Pear samples exhibited the highest contamination levels, with maximum residue level (MRL) exceedance rates reaching 30%, compared to 14.5% in apples and 2% in quinces. Quality assessment based on the index of quality for residues (IqR) indicated that 96% of quince samples were classified as excellent or good, demonstrating the most favourable profile among the evaluated commodities. Risk ranking analysis further indicated that acetamiprid was the only high-risk pesticide in apples, whereas residues in pears were predominantly medium risk, and all detected compounds in quinces fell within the low-risk category. Deterministic risk assessment indicated that chronic exposure remained well below levels of concern for both adults and children. Under combined pome fruit consumption, acetamiprid and spirodiclofen were identified as the main contributors to chronic hazard index (HIc), accounting for 33% and 13% of HIc, respectively. However, acute exposure exceeded the safety threshold (HQa > 1) in children for acetamiprid in both apples and pears. Probabilistic modelling confirmed right-skewed exposure distributions and highlighted increased risk under cumulative consumption scenarios. Full article
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18 pages, 367 KB  
Review
Integrated Management of Cydia pomonella Within a One Health Perspective: A Global Review
by Roberta Duarte Ávila Vieira, Bruna Fernanda da Silva and Lenita Agostinetto
Green Health 2026, 2(2), 15; https://doi.org/10.3390/greenhealth2020015 - 2 Jun 2026
Viewed by 395
Abstract
Cydia pomonella (Linnaeus, 1758) is considered one of the major pests affecting global pome fruit production due to its wide distribution, cryptic feeding habit, high economic impact, and continuous evolution of insecticide resistance. Historically, management of this species has relied on repeated pesticide [...] Read more.
Cydia pomonella (Linnaeus, 1758) is considered one of the major pests affecting global pome fruit production due to its wide distribution, cryptic feeding habit, high economic impact, and continuous evolution of insecticide resistance. Historically, management of this species has relied on repeated pesticide applications, which have been associated with environmental impacts, occupational exposure, pesticide residues in food, and compromised sustainability of pesticide-dependent agricultural systems, reinforcing the relevance of integrated One Health approaches. This narrative review analyzed global management strategies for C. pomonella published between 2014 and 2024 and indexed in the Scopus, Web of Science, and SciELO databases. The reviewed studies demonstrate a gradual transition from predominantly chemical-based programs toward integrated strategies involving pheromone-based monitoring, mating disruption, biological control, and preventive plant biosecurity measures. Behavioral and biological approaches showed potential to reduce dependence on recurrent insecticide applications, particularly when associated with phytosanitary surveillance and integrated pest management programs. However, the effectiveness of these approaches remains influenced by insecticide resistance, climatic variability, and local ecological conditions. The evidence also suggests that the impacts of C. pomonella management are not limited to phytosanitary protection, involving interactions related to environmental sustainability, food safety, and human exposure to pesticides. Despite the relevance of the One Health approach, its operational incorporation into agricultural pest management remains limited, especially regarding the integration of research conducted under the One Health perspective. In this context, the sustainable management of Cydia pomonella requires integrated strategies capable of connecting phytosanitary surveillance, preventive plant biosecurity, and agricultural and ecological sustainability in order to ensure food security and population health. Full article
16 pages, 1261 KB  
Review
Advances in Fruit Organic Acid Metabolism and Molecular Regulation in Fruit Trees
by Xufeng Guo, Yanxia Zhang, Zhenghai Liu, Min Tan, Jinyu He, Qifeng Zhao and Zhigang Dong
Horticulturae 2026, 12(5), 566; https://doi.org/10.3390/horticulturae12050566 - 5 May 2026
Viewed by 1253
Abstract
Organic acids are the core of fruit flavor quality and cell metabolism, but a comprehensive review of their metabolism and regulatory networks in fruit trees is still limited. Here, we systematically summarized the biosynthesis, degradation and transport of major organic acids in fruits [...] Read more.
Organic acids are the core of fruit flavor quality and cell metabolism, but a comprehensive review of their metabolism and regulatory networks in fruit trees is still limited. Here, we systematically summarized the biosynthesis, degradation and transport of major organic acids in fruits of horticultural crops. We focused on the distribution and molecular regulation of organic acids in citrus, pome fruits, stone fruits, grapes and tropical–subtropical fruits, and emphasized the regulation of transcription factors, epigenetic modifications and environmental signals. We also evaluated the progress of various omics strategies for dissecting organic acid metabolism and identifying key regulatory genes. Finally, we discuss the current research gaps and propose future directions for multi-gene editing and molecular design breeding. This review provides a theoretical framework for improving fruit flavor quality and breeding excellent varieties. Full article
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39 pages, 1486 KB  
Review
An Overview of Major Penicillium Species Associated with Plant Diseases
by Latiffah Zakaria
J. Fungi 2026, 12(4), 286; https://doi.org/10.3390/jof12040286 - 17 Apr 2026
Cited by 2 | Viewed by 1884
Abstract
Species of Penicillium are among the most important fungal pathogens responsible for postharvest diseases of agricultural crops worldwide. This review provides an overview of five economically important Penicillium spp., namely P. expansum, P. digitatum, P. italicum, P. citrinum, and [...] Read more.
Species of Penicillium are among the most important fungal pathogens responsible for postharvest diseases of agricultural crops worldwide. This review provides an overview of five economically important Penicillium spp., namely P. expansum, P. digitatum, P. italicum, P. citrinum, and P. oxalicum. Emphasis is placed on P. expansum, P. digitatum, and P. italicum which are the main causal agents of blue mold and green mold rots in pome fruits and citrus, commodities that dominate global fresh produce trade and long-term storage. While studies on plant-pathogenic Penicillium are mainly focused on these hosts, this review highlights reports of infections in other crops across diverse geographic regions, highlighting the broader host range of these species. The main aspects highlighted include host specificity and diversity, production of mycotoxins and other secondary metabolites, current management and control strategies, and the potential influence of climate change on disease incidence and severity. Understanding the biology and epidemiology of plant-pathogenic Penicillium species is essential, as several species are both pathogens and producers of mycotoxins, leading to quality deterioration and nutrient depletion resulting in economic losses. Full article
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27 pages, 336 KB  
Article
Replacing Glyphosate Shifts Environmental Burdens: Trade-Offs Between Ecotoxicity and Climate Impact in Chemical and Non-Chemical Strategies
by Michael Raimondi, Edelbis López Dávila, Laura Peeters, Wim Reybroeck, Tim Belien, Dany Bylemans, Jeroen Buysse, Benny De Cauwer and Pieter Spanoghe
Agronomy 2026, 16(5), 510; https://doi.org/10.3390/agronomy16050510 - 26 Feb 2026
Viewed by 2343
Abstract
The potential withdrawal of glyphosate necessitates a comprehensive evaluation of alternative weed control strategies that balances human health safety with environmental concerns. This study applied a decision-support grid to compare the impacts of glyphosate-based reference strategies against chemical and non-chemical alternatives across four [...] Read more.
The potential withdrawal of glyphosate necessitates a comprehensive evaluation of alternative weed control strategies that balances human health safety with environmental concerns. This study applied a decision-support grid to compare the impacts of glyphosate-based reference strategies against chemical and non-chemical alternatives across four Belgian case studies: pome fruit orchards, grassland renewal, arable weed patches, and railways. The assessment integrated twelve risk indicators including human, environmental and biodiversity risk, and life cycle assessment for global warming potential (GWP) into a Final Scenario Score (FSS). The results indicated that only one alternative strategy, the chemical alternative in local weed patch control, achieved the FSS threshold (<0.75) required to justify substitution (FSS = 0.70). Chemical alternatives in other case studies frequently shifted burdens; for instance, bio-herbicides in railways increased risks to residents and aquatic organisms compared to the reference. Conversely, mechanical and thermal alternatives eliminated chemical toxicity but resulted in GWP increases up to 32 times higher than glyphosate-based practices. These findings demonstrate that chemical substitutes often maintain toxicity risks while non-chemical strategies trade them for increased climate impacts. Consequently, a ban on glyphosate is currently unsupported by the environmental performance of available alternatives in these temperate high-intensity systems. Sustainable progress requires a transition period where optimized conventional strategies remain available within integrated weed management, while innovations in electrification and precision technology are accelerated to resolve current trade-offs. Full article
(This article belongs to the Special Issue Herbicide Use: Effects on the Agricultural Environment)
17 pages, 295 KB  
Article
Pesticide Residues in Apples and Pears: A Deterministic Assessment of Chronic Exposure and Non-Carcinogenic Risk for European Consumers
by Jarosław Chmielewski, Barbara Gworek, Magdalena Florek-Łuszczki and Jarogniew J. Łuszczki
Molecules 2026, 31(5), 767; https://doi.org/10.3390/molecules31050767 - 25 Feb 2026
Cited by 1 | Viewed by 1151
Abstract
(1) Pome fruits (apples and pears) are among the most frequently consumed fruits in Europe and may contribute to dietary exposure to pesticide residues. Although residue levels generally comply with maximum residue limits (MRLs), even low concentrations may cumulatively contribute to chronic health [...] Read more.
(1) Pome fruits (apples and pears) are among the most frequently consumed fruits in Europe and may contribute to dietary exposure to pesticide residues. Although residue levels generally comply with maximum residue limits (MRLs), even low concentrations may cumulatively contribute to chronic health risks under conditions of frequent and long-term consumption. This study aimed to quantitatively assess dietary exposure and the potential non-carcinogenic health risks associated with pesticide residues in apples and pears, using representative monitoring and consumption data. (2) The assessment was based on results of the Polish national official monitoring program for pesticide residues in food, specifically apples and pears sampled in 2022, as reported by the National Institute of Public Health (NIZP-PZH). These data were combined with age- and body weight-specific consumption scenarios derived from FAO/WHO GEMS/Food cluster diets and national Polish statistics. For the most frequently detected pesticides (captan, flonicamid, acetamiprid and fosetyl-Al in apples; captan and acetamiprid in pears), the mean and 95th percentile concentrations were used to estimate the estimated daily intake (EDI). Non-carcinogenic risk was characterized using the hazard quotient (HQ = EDI/ADI) and the cumulative Hazard Index (HI). The hazard quotient (HQ) was calculated as the ratio of estimated daily intake to the acceptable daily intake (HQ = EDI/ADI), while the Hazard Index (HI) was defined as the sum of individual HQ values for pesticides detected in a given commodity and exposure scenario (HI = ΣHQ). Calculations were performed separately for children and adults under several dietary scenarios (Polish general population, German child, German general population, GEMS/Food G08). (3) For all pesticides and exposure scenarios, the HQ values were well below 1, indicating no exceedance of the acceptable daily intake (ADI). The highest chronic exposure was observed for apples in children (German child scenario), with the HQ values for captan, flonicamid and acetamiprid in the approximate range of 0.01–0.05, while the HI remained < 0.1 even under high-consumption conditions. In adults (Polish and German general populations, GEMS/Food G08), HQ values were approximately one order of magnitude lower than in children, and the cumulative HI values for both apples and pears were far below 1. The contribution of pears to total exposure was limited, reflecting lower consumption and fewer active substances detected. (4) This quantitative risk assessment, based on Polish monitoring data from 2022, indicates that under current residue levels and consumption patterns, chronic dietary exposure to pesticide residues from apples and pears does not pose a relevant non-carcinogenic health concern for either children or adults. Nevertheless, children consistently showed higher relative exposure than adults, underscoring the importance of age-stratified risk assessment and continued monitoring of residues in commonly consumed fruits. The findings support existing regulatory frameworks while justifying sustained, targeted surveillance of key active substances in pome fruits as part of public health prevention strategies. Full article
33 pages, 2600 KB  
Review
A Comprehensive Review of Biochemical Insights and Advanced Packaging Technologies for Shelf-Life Enhancement of Temperate Fruits
by Sharath Kumar Nagaraja, Puneet Kumar, Kavitha R, Sajad Un Nabi, Javid Iqbal Mir, Mahendra Kumar Verma, Ozgun Kalkisim, Mustafa Akbulut, Yong Beom Kwon, Ho-Min Kang and Sheikh Mansoor
Biosensors 2026, 16(2), 94; https://doi.org/10.3390/bios16020094 - 2 Feb 2026
Cited by 2 | Viewed by 1552
Abstract
Temperate fruits, mostly comprising pome, stone fruits, and berries with immense nutritional benefits and a storehouse of various therapeutic phytochemicals, are prone to several physiological disorders immediately after harvest. The etiology, symptom progression, and decay incidence are influenced by pre-harvest and post-harvest factors, [...] Read more.
Temperate fruits, mostly comprising pome, stone fruits, and berries with immense nutritional benefits and a storehouse of various therapeutic phytochemicals, are prone to several physiological disorders immediately after harvest. The etiology, symptom progression, and decay incidence are influenced by pre-harvest and post-harvest factors, causing significant economic loss with respect to both the energy and economics invested. Respiratory end products, ethylene generation, and enzymatic activities interact to influence the metabolic response and associated biochemical variation. Advanced packaging technologies have emerged as innovative solutions to curtail these post-harvest problems. The design and development of novel packaging technologies need to critically understand the respiratory behavior of the fruits and their associated metabolic functions. A desirable polymer or packaging technology should exhibit enhanced barriers to the gases while providing adequate support to the fruit matrix. In addition, it should also fulfill the role of environmental sustainability and the circular economy. The outcome of this review will highlight the importance of proper post-harvest procedure, appropriate pretreatment, packaging matrix selection, and the storage conditions for effective and enhanced shelf-life storage. Therefore, this review was structured in two phases; the first phase discusses the biochemical understanding of the fruit during storage and transit in response to stress factors. The next phase highlights the various packaging interventions (polymers, biodegradable films, edible coatings, smart packaging, nano-packaging) taken to address these issues, with a key focus on shelf-life enhancement. Further, the key limitations of each technology are appraised. Full article
(This article belongs to the Section Environmental, Agricultural, and Food Biosensors)
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23 pages, 3299 KB  
Systematic Review
Utilization of Oil Palm Residual Biomass Within the Framework of Industrial Symbiosis: A Systematic Review of the Economic Sectors Involved in Its Valorization
by Dalidys Rendón-Camargo, Efrain Boom-Cárcamo, Lina Buelvas-Gutiérrez and Ana Maya-Gonzalez
Biomass 2026, 6(1), 10; https://doi.org/10.3390/biomass6010010 - 2 Feb 2026
Cited by 5 | Viewed by 2283
Abstract
This study analyzes the valorization of oil palm biomass residues within the framework of industrial symbiosis (IS), emphasizing their role in circular economy strategies and sustainable industrial development. Through a systematic literature review and snowball sampling, 156 articles indexed in Scopus and Web [...] Read more.
This study analyzes the valorization of oil palm biomass residues within the framework of industrial symbiosis (IS), emphasizing their role in circular economy strategies and sustainable industrial development. Through a systematic literature review and snowball sampling, 156 articles indexed in Scopus and Web of Science were examined, classifying evidence by country, type of residue, derived products, economic sector (ISIC Rev. 4), and technological approach. The results show a strong geographical concentration of IS experiences in Asia, particularly Malaysia, Indonesia, and Thailand, where residues such as empty fruit bunches (EFB), palm kernel shells (PKS), oil palm mesocarp fibers, palm oil mill effluent (POME), and oil palm trunks (OPT) are integrated into processes for bioenergy, biochemicals, composite materials, construction products, biochar, and bioplastics. In contrast, applications in Latin America and Africa remain incipient, with high potential but limited industrial implementation due to infrastructural and regulatory gaps. Technological trends point toward thermo-chemical and biological conversion routes (pyrolysis, gasification, hydrothermal carbonization, anaerobic digestion), development of advanced materials and catalysts, and the emergence of integrated biorefinery models supported by computational optimization tools. The analysis highlights that palm biomass residues, far from being an environmental liability, constitute strategic resources for low-carbon value chains. However, scaling IS initiatives requires clear public policies, economic incentives, and stronger coordination between industry, government, and academia. The study provides a structured overview of current knowledge, identifies research gaps, and outlines future directions for leveraging oil palm residues as a key input for sustainable IS. Full article
(This article belongs to the Topic The Utilization of Non-Grain Biomass Resources)
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18 pages, 2460 KB  
Article
Techno-Economic and FP2O Resilience Analysis of the Hydrogen Production Process from Palm Rachis in María La Baja, Bolívar
by Tamy Carolina Herrera-Rodríguez, Paola Andrea Acevedo Pabón and Ángel Darío González-Delgado
Processes 2026, 14(3), 489; https://doi.org/10.3390/pr14030489 - 30 Jan 2026
Viewed by 814
Abstract
In Colombia, two main palm varieties, Elaeis guineensis and Elaeis oleifera, are cultivated for the production of crude palm oil (CPO). During the CPO extraction process, several residues are generated, including empty fruit bunches (EFB), nut fiber, palm kernel cake, and Palm [...] Read more.
In Colombia, two main palm varieties, Elaeis guineensis and Elaeis oleifera, are cultivated for the production of crude palm oil (CPO). During the CPO extraction process, several residues are generated, including empty fruit bunches (EFB), nut fiber, palm kernel cake, and Palm Oil Mill Effluent (POME), among others. These residues are commonly used for biochar and compost production to improve soil quality, for biogas generation, and for energy production through biomass combustion. Because the rachis is rich in lignocellulosic material and exhibits physicochemical properties suitable for thermochemical processes, it is proposed as a feedstock for hydrogen synthesis through gasification. In this study, a techno-economic analysis and an FP2O resilience assessment were conducted for a hydrogen production process based on the utilization of palm rachis generated in María la Baja, northern Colombia. The economic evaluation results indicate that the capital investment required for plant installation is USD 10,111,255.23. The economic indicators show favorable performance with a Return on Investment (ROI) of 58.83%, a Net Present Value (NPV) of USD 25.01 million, a B/C ratio of 3.29, and a Discounted Payback Period (DPBP) of 4.54 years. Regarding techno-economic resilience, critical values for processing capacity, selling price, and feedstock cost were identified through parameter variation. The findings suggest that the process has opportunities for improvement, since small changes in these variables could significantly reduce its resilience. Finally, an On-Stream efficiency of 39.65% at the break-even point was obtained, indicating that the process can operate at less than 50% of its maximum capacity while still generating significant profits. Full article
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27 pages, 3474 KB  
Article
Exploring the Possible Role of Semiochemicals in Quince (Cydonia oblonga Mill.): Implications for the Biological Behavior of Cydia pomonella
by María Pía Gomez, Flavia Jofré Barud, Sayra Jaled, Silvina Garrido, Liliana Cichón and María Liza López
Agronomy 2026, 16(3), 331; https://doi.org/10.3390/agronomy16030331 - 28 Jan 2026
Viewed by 631
Abstract
The codling moth (Cydia pomonella L.) is a major pest of pome fruits worldwide, guided by semiochemicals to locate hosts and oviposition sites. Quince (Cydonia oblonga Mill.), although less studied, is also affected by this pest. This study aimed to identify [...] Read more.
The codling moth (Cydia pomonella L.) is a major pest of pome fruits worldwide, guided by semiochemicals to locate hosts and oviposition sites. Quince (Cydonia oblonga Mill.), although less studied, is also affected by this pest. This study aimed to identify behaviorally active compounds for codling moth by characterizing the volatilome of quince cultivars. Volatile profiles were analyzed across four phenological stages (flowering, unripe, growth, and ripe fruit) using solid-phase microextraction and GC–MS. The cultivars evaluated were Champion, INTA 37, INTA 117, and INTA 147. Female oviposition behavior and neonate larval host choice were also assessed. Identified volatiles included esters, sesquiterpenes, monoterpenes, alcohols, aldehydes, and norisoprenoids. Among monoterpenes, limonene, consistently detected across all cultivars and stages, emerged as a key kairomone. Volatile composition varied across phenological stages, with the fruit growth stage exhibiting the highest diversity and abundance of compounds previously reported as behaviorally active. This pattern coincided with peak female oviposition and larval host selection. Females oviposited mainly on leaf surface, whereas during ripening, eggs were deposited on fruit lacking pubescence. Overall, INTA 147 was the most preferred cultivar. These findings highlight quince volatiles, particularly Limonene, as potential candidates for the development of semiochemical-based tools to improve codling moth management. Full article
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17 pages, 9792 KB  
Article
Quantifying Key Environmental Determinants Shaping the Ecological Niche of Fruit Moth Carposina sasakii Matsumura, 1900 (Lepidoptera, Carposinidae)
by Ziyu Huang, Ling Wu, Huimin Yao, Shaopeng Cui, Angie Deng, Ruihe Gao, Fei Yu, Weifeng Wang, Shiyi Lian, Yali Li, Lina Men and Zhiwei Zhang
Insects 2026, 17(1), 109; https://doi.org/10.3390/insects17010109 - 18 Jan 2026
Cited by 1 | Viewed by 810
Abstract
Carposina sasakii Matsumura is a significant lepidopteran pest in the Carposinidae family, inflicting substantial damage on stone and pome fruit trees such as jujube, peach, and apple. Using MaxEnt, we assessed the worldwide climatic suitability for C. sasakii and its key environmental drivers, [...] Read more.
Carposina sasakii Matsumura is a significant lepidopteran pest in the Carposinidae family, inflicting substantial damage on stone and pome fruit trees such as jujube, peach, and apple. Using MaxEnt, we assessed the worldwide climatic suitability for C. sasakii and its key environmental drivers, evaluating how climate change impacts dispersal risks. Integrating global occurrence records with 37 environmental variables, the model (AUC = 0.982) quantitatively identifies July precipitation (prec7), minimum average temperatures in April and August (tmin4 and tmin8, respectively), and maximum average temperature in May (tmax5) as critical distribution determinants. Among these, prec7 exhibits the highest contribution (threshold approximately 370 mm). The current suitable habitat spans 10.39 × 102 km2, concentrated predominantly in East Asia’s temperate monsoon zone (eastern China, the Korean Peninsula, and Japan) and southern North America. Under future climate scenarios, the high-emission pathway (SSP585) will reduce highly suitable areas, while moderately suitable zones expand coastward. In contrast, SSP370 projects a significant, albeit phased, habitat increase with a 19.61% growth rate. Precipitation regimes and extreme temperatures jointly regulate niche differentiation in C. sasakii, whose range shifts toward Southeast Asia and suboptimal regions in Europe and America, underscoring cascading climate change effects. These findings provide a scientific basis for transnational monitoring, early warning systems, and regional ecological governance. Full article
(This article belongs to the Section Insect Pest and Vector Management)
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14 pages, 488 KB  
Article
Using a Standardized Protocol to Assess Female Codling Moth, Cydia pomonella (L.), Mating Status Under Mating Disruption Technologies
by Alan Lee Knight, Michele Preti and Esteban Basoalto
Insects 2026, 17(1), 99; https://doi.org/10.3390/insects17010099 - 15 Jan 2026
Cited by 2 | Viewed by 872
Abstract
Implementing mating disruption (MD) programs to manage codling moth (CM), Cydia pomonella (L.), should be based on knowledge of how effectively each program disrupts female mating. A recent survey of 142 pome fruit orchards under MD in Washington State and Oregon found that, [...] Read more.
Implementing mating disruption (MD) programs to manage codling moth (CM), Cydia pomonella (L.), should be based on knowledge of how effectively each program disrupts female mating. A recent survey of 142 pome fruit orchards under MD in Washington State and Oregon found that, on average, about half of the CM females caught in traps baited with a kairomone-based lure were mated. However, significantly lower proportions of mated females were sampled when the intensity of the MD program was increased. A standardized protocol that could reduce the large inter-orchard variability was developed, involving weekly releases of sterilized CM adults. Eleven trials were conducted in 2023 and 2024 across 82 orchards treated with 20 MD programs. The intensive MD programs were significantly more effective in reducing mating of both wild and sterile CM females. Three advantages of using sterile moths to assess CM MD were identified: (i) it minimized the impact of wild immigrant females or individuals previously exposed to sublethal spray residues; (ii) it allowed greater numbers of females to be dissected, thus increasing the precision of the mean value; and (iii) it and allowed the collection of sufficient sampling data (>5 CM females per site) from 30% more orchards than relying on wild moth catch. Full article
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26 pages, 4221 KB  
Article
Predicting Phenological Stages for Cherry and Apple Orchards: A Comparative Study with Meteorological and Satellite Data
by Valentin Kazandjiev, Dessislava Ganeva, Eugenia Roumenina, Georgi Jelev, Veska Georgieva, Boryana Tsenova, Petia Malasheva, Marieta Nesheva, Svetoslav Malchev, Stanislava Dimitrova and Anita Stoeva
Agronomy 2026, 16(2), 200; https://doi.org/10.3390/agronomy16020200 - 14 Jan 2026
Viewed by 1345
Abstract
Fruit growing is a traditional component of Bulgarian agricultural production. According to the latest statistical data, the share of areas planted with cherries is 10.5% of the total orchard area, and with apples, 7.2%, totaling 67,800 ha. This article presents the results of [...] Read more.
Fruit growing is a traditional component of Bulgarian agricultural production. According to the latest statistical data, the share of areas planted with cherries is 10.5% of the total orchard area, and with apples, 7.2%, totaling 67,800 ha. This article presents the results of ground and remote (satellite) measurements and observations of cherry and apple orchards, along with the methods for their processing and interpretation, to define the current state and forecast their expected development. This research aims to combine the capabilities of the two approaches by improving and expanding observation and forecasting activities. Ground-based measurements and observations consider the dates of a permanent transition in air temperature above 5 °C and several cardinal phenological stages, based on the idea that a certain temperature sum (CU, GDH, GDD) must accumulate to move from one phenological stage to another. The obtained data were statistically analyzed, and by means of classification with the Random Forest algorithm, the dates for the occurrence of the stages of bud break, flowering, and fruit ripening in the development of cherry and apple orchards were predicted with an accuracy of −6 to +2 days. Satellite studies include creating a database of Sentinel-2 digital images across different spectral bands for the studied orchards, investigating various post-processing approaches, and deriving indicators of developmental phenostages. Ground data from the 2021–2023 experiment in Kyustendil and Plovdiv were used to determine the phases of fruit bursting, flowering, and ripening through satellite images. An assessment of the two approaches to predicting the development of the accuracy of the models was carried out by comparing their predictions for bud swelling and bursting (BBCH 57), flowering (BBCH 65), and fruit ripening (BBCH 87/89) of the observed phenological events in the two selected orchard types, representatives of stone and pome fruit species. Full article
(This article belongs to the Section Innovative Cropping Systems)
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15 pages, 13736 KB  
Article
Molecular Survey and Genetic Characterization of Hop Stunt Viroid (HSVd) in Fruit Trees in Kazakhstan
by Leila T. Nadirova, Gulshan E. Stanbekova, Anna S. Nizkorodova, Ruslan V. Kryldakov, Bulat K. Iskakov and Andrey V. Zhigailov
Viruses 2025, 17(12), 1547; https://doi.org/10.3390/v17121547 - 26 Nov 2025
Cited by 1 | Viewed by 873
Abstract
Hop stunt viroid (HSVd) infects a variety of natural hosts, including fruit trees, leading to significant economic losses worldwide. This survey aimed to assess the incidence of HSVd in fruit trees in southern Kazakhstan. Out of 482 fruit trees examined, 28 (5.81%) were [...] Read more.
Hop stunt viroid (HSVd) infects a variety of natural hosts, including fruit trees, leading to significant economic losses worldwide. This survey aimed to assess the incidence of HSVd in fruit trees in southern Kazakhstan. Out of 482 fruit trees examined, 28 (5.81%) were found to be infected with HSVd. The incidence was significantly higher in stone fruit trees (8.15%; 22/270) compared to pome fruit trees (2.82%; 6/212; p = 0.0133). Apricots had the highest infection rate at 12.66% (10/79), while pears had the lowest rate at 2.08% (1/48). Fifteen of the identified viroids were cloned for full-genome sequencing. Sequence analysis revealed a high percentage of nucleotide sequence similarity (99–100%) among the Kazakhstani HSVd isolates, suggesting a possible unique origin for the infection. We also identified several SNPs of HSVd that have not been previously documented. A phylogenetic analysis indicated that the Kazakhstani HSVd variants clustered together in a separate group, distinct from the five known groups of HSVd. This potentially new group displayed differences in its predicted secondary structure compared to other viroid groups. These findings emphasize the need for the development of effective control measures for HSVd and other viroids affecting fruit trees. Full article
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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14 pages, 1526 KB  
Article
Antibiotic and Copper Sensitivity in Erwinia amylovora Isolates from Northern Saudi Arabia, and the Induction of Fire Blight Suppression by Salicylic Acid
by Ali A. Al Masrahi, Abdurrehman M. Rafique, Abdullah F. Al Hashel, Mohammed A. Al Saleh and Yasser E. Ibrahim
Plants 2025, 14(20), 3192; https://doi.org/10.3390/plants14203192 - 17 Oct 2025
Viewed by 1381
Abstract
Fire blight, caused by Erwinia amylovora, is a severe disease impacting pome fruit production worldwide, including in Saudi Arabia. This study evaluated antibiotic sensitivity and the potential of chemical and elicitor treatments to suppress E. amylovora isolates collected from various regions in [...] Read more.
Fire blight, caused by Erwinia amylovora, is a severe disease impacting pome fruit production worldwide, including in Saudi Arabia. This study evaluated antibiotic sensitivity and the potential of chemical and elicitor treatments to suppress E. amylovora isolates collected from various regions in Saudi Arabia. In the in vitro assays, at low antibiotic levels (10 µg/mL streptomycin and 25 µg/mL oxytetracycline), all Saudi Arabian strains exhibited minimal inhibition (zones ≤ 14 mm). Two isolates displayed partial tolerance at an intermediate oxytetracycline concentration (50 µg/mL). True sensitivity (zones > 18 mm) was mainly observed at the highest tested oxytetracycline dose (100 µg/mL). Regarding copper sulfate, all isolates showed no inhibition between 0.02 and 0.08 mM, while all isolates exhibited intermediate susceptibility at 0.16 mM. The second experimental phase examined in planta effects of streptomycin, salicylic acid (SA), and their combination on disease development in artificially inoculated apple (Malus domestica) shoots under greenhouse conditions. Both streptomycin and SA significantly reduced fire blight incidence (by 75%) and symptom severity, while the combined treatment yielded the greatest reduction in shoot necrosis and bacterial load. This is the first report demonstrating that SA, particularly when used in combination with streptomycin, can effectively suppress fire blight in Saudi Arabia. These results stress the importance of integrating resistance inducers into fire blight management strategies to counter the rise in antimicrobial resistance. Full article
(This article belongs to the Special Issue Occurrence and Control of Plant Bacterial Diseases)
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