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28 pages, 4082 KiB  
Review
Perspectives in the Scientific Literature on the Barriers and Benefits of the Transition to a Plant-Based Diet: A Bibliometric Analysis
by Lelia Voinea, Ana-Maria Badea, Răzvan Dina, Dorin Vicențiu Popescu, Mihaela Bucur and Teodor Mihai Negrea
Foods 2025, 14(17), 2942; https://doi.org/10.3390/foods14172942 (registering DOI) - 23 Aug 2025
Abstract
Plant-based diets are increasingly attracting attention as they play a significant role in human health and environmental sustainability and are believed to be key components of sustainable food systems. In the present study, both pros and cons of the adoption of plant-based diets [...] Read more.
Plant-based diets are increasingly attracting attention as they play a significant role in human health and environmental sustainability and are believed to be key components of sustainable food systems. In the present study, both pros and cons of the adoption of plant-based diets are analyzed using a bibliometric method integrated with a qualitative examination of the scientific literature. For the bibliometric study, Bibliometrix software was utilized, examining 3245 scientific articles, downloaded from the Scopus database, and printed between the years 1957 and 2025. The analyses were conducted using R software, version 4.4.1, with access to the Bibliometrix package, version 4.1. The results indicate a remarkable rise, in the last two decades, in the scholarly focus on the influence of plant-based diets on the individual’s health condition as well as the environment. Keyword co-occurrence studies and international collaborations demonstrate a dominance of research focus in both the United States and Europe, with significant contributions from the Asia–Pacific region. Furthermore, the current work offers qualitative identification of the benefits of plant diets from various perspectives like nutritional, economic, ecological, and cultural. It also explores the main dissuaders from adhering to these diets, including perceived nutritional hazards, cost perceptions, low availability, and social prohibitions. Findings emphasize that, in spite of all the barriers, plant food-based diets have a wide-ranging ability to provide tangible benefits at both the individual and population levels, and documented in the scientific literature are recommendations of expert-led education programs, economic incentives, and judiciously framed public policies to overcome these barriers and to make this transition possible towards sustainable food choices. Findings provide a comprehensive understanding of the current lines of inquiry and stage the subsequent work on how to motivate sustainability among the general population. Full article
(This article belongs to the Section Food Security and Sustainability)
14 pages, 1508 KiB  
Article
Analysis of Coupled Response Characteristics of NAI Release and Stem Flow in Four Urban Greening Tree Species in Beijing During Drought Stress and Recovery Processes
by Xueqiang Liu, Bin Li, Weikang Zhang, Shaowei Lu, Jigui Wu, Jing An, Yaqian Fan, Na Zhao, Xiaotian Xu and Shaoning Li
Plants 2025, 14(17), 2630; https://doi.org/10.3390/plants14172630 (registering DOI) - 23 Aug 2025
Abstract
Negative air ions (NAI) represent an important ecological value indicator for green tree species. Flow of sap is a crucial indicator for water utilization and physiological state of trees. Although there have been some advancements in studies on the correlation between the release [...] Read more.
Negative air ions (NAI) represent an important ecological value indicator for green tree species. Flow of sap is a crucial indicator for water utilization and physiological state of trees. Although there have been some advancements in studies on the correlation between the release of NAI by plants and sap flow in recent years, it is still unclear how the release of NAI by plants changes during drought stress and recovery processes, as well as the coupling effect between the release of NAI by plants and sap flow under drought stress. In this context, four typical green tree species, Robinia pseudoacacia, Quercus variabilis, Pinus tabulaeformis, and Platycladus orientalis, were selected as experimental materials. A drought stress and recovery control experiment was conducted based on OTC. The dynamic data of negative air ion concentration (NAIC) and sap flow rate during the process of drought stress and recovery were monitored to clarify the characteristics and correlations of NAI and sap flow changes in the experimental tree species under drought stress and recovery. The main research results are as follows: (1) At the end of the drought period, the NAI and sap flow in the drought treatment group significantly decreased (p < 0.01), compared with the control group (CK), and the reduction rate of sap flow (77.73 ± 4.96%) for each tree species was higher than that of NAI (47.78% ± 4.96%). (2) At 1 day after rehydration, the recovery amplitudes of NAI and sap flow for all tree species were the greatest; at 7 days after rehydration, the NAI and sap flow of the drought treatment group recovered to the levels of the control group (p > 0.05). (3) During different stages of drought rehydration, the response degree of NAI to sap flow varied. The study found that in the drought-rehydration stage, the correlation between the NAI released by each tree species and sap flow was the lowest at the drought endpoint. In conclusion, this research clarifies the changing patterns of plant NAI release and sap flow during drought-rehydration, as well as the response changes of NAI to sap flow. It provides a theoretical basis for selecting drought-tolerant tree species in arid regions. Full article
(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)
33 pages, 37073 KiB  
Article
Optimized Plant Configuration Designs for Wind Damage Prevention in Masonry Heritage Buildings: A Case Study of Zhen Guo Tower in Weihui, Henan, China
by Zhiyuan Mao, Ke Ma, Dong He, Zhenkuan Guo, Xuefei Zhao and Yichuan Zhang
Buildings 2025, 15(17), 2999; https://doi.org/10.3390/buildings15172999 (registering DOI) - 23 Aug 2025
Abstract
Wind-induced erosion and extreme weather events pose growing risks to the structural integrity of masonry heritage buildings. However, current mitigation approaches often overlook ecological sustainability. This study investigates the wind-regulating effects of vegetation surrounding the Zhen Guo Tower, a 400-year-old masonry structure in [...] Read more.
Wind-induced erosion and extreme weather events pose growing risks to the structural integrity of masonry heritage buildings. However, current mitigation approaches often overlook ecological sustainability. This study investigates the wind-regulating effects of vegetation surrounding the Zhen Guo Tower, a 400-year-old masonry structure in Weihui, Henan Province, China. Using computational fluid dynamics (CFD) simulations, we first assess the protective performance of the existing vegetation layout and then develop and evaluate an optimized plant configuration. The results show that the proposed multilayered vegetation arrangement effectively reduces wind speeds by up to 13.57 m/s under extreme wind conditions, particularly within the 5–15 m height range. Wind protection efficiency improved by 28–68% compared to the baseline. This study demonstrates a replicable and ecologically integrated strategy for mitigating wind hazards in masonry heritage sites through vegetation-based interventions. Full article
(This article belongs to the Section Building Structures)
32 pages, 456 KiB  
Review
Emerging Approaches to Anthelmintic Therapy Using Medicinal Plants and Phytochemicals: A Review of Natural Products Against Strongyloidiasis
by Julio López-Abán, Belén Vicente-Santiago, Guadalupe Gutiérrez-Soto, Nancy Edith Rodríguez-Garza, Miroslava Kačániová, Iosvany López-Sandin, Cesar Iván Romo-Sáenz, Juan Manuel Ballesteros-Torres, Lucio Galaviz-Silva, Uziel Castillo-Velázquez, Stefania Garzoli and Joel Horacio Elizondo-Luévano
Pathogens 2025, 14(9), 842; https://doi.org/10.3390/pathogens14090842 (registering DOI) - 23 Aug 2025
Abstract
Strongyloidosis is a parasitic disease caused by Strongyloides stercoralis, a nematode with a complex life cycle that facilitates long-term persistence within the host. The infection affects millions of people in tropical and subtropical regions and poses a particular challenge in immunocompromised individuals. [...] Read more.
Strongyloidosis is a parasitic disease caused by Strongyloides stercoralis, a nematode with a complex life cycle that facilitates long-term persistence within the host. The infection affects millions of people in tropical and subtropical regions and poses a particular challenge in immunocompromised individuals. Although conventional treatments, such as ivermectin and albendazole, are generally effective, emerging concerns regarding drug resistance and adverse effects have prompted the search for alternative therapeutic options. In this context, natural products—including plant extracts, bioactive phytochemicals, and nanoparticle-based formulations derived from natural sources—are emerging as promising anti-Strongyloides potential. This review summarizes recent studies on natural products with anthelmintic activity against strongyloidiasis, with emphasis on their mechanisms of action, efficacy, and future perspectives. A systematic search of the literature was conducted using terms related to Strongyloides, plant species, extracts, and bioactive compounds with nematocidal activity. Eligible studies included those reporting the activity of plants, plant extracts, and their purified metabolites against Strongyloides spp. Data were compiled into a comprehensive table including year of publication, author, plant species, active principle, application conditions, and target nematode species. The pharmacological treatment of this parasite varies according to its life cycle stage. Various biomolecules, phytoactive compounds, and novel plant-based formulations have demonstrated promising activity and may be considered both for treatment and for inclusion in control programs for strongyloidiasis. This review highlights medicinal plants and phytochemicals with ethnopharmacological background and experimentally validated activity against Strongyloides spp., integrating evidence from in vitro, in vivo, and experimental models, as well as clinical trials. Full article
(This article belongs to the Special Issue Parasitic Helminths and Control Strategies)
13 pages, 1260 KiB  
Article
Seasonal Uptake and Partitioning of Macro- and Micronutrients in Yellow-Fleshed Kiwifruit (Actinidia chinensis var. chinensis)
by Elena Baldi, Maurizio Quartieri, Giovambattista Sorrenti, Marco Mastroleo, Evangelos Xylogiannis and Moreno Toselli
Horticulturae 2025, 11(9), 1003; https://doi.org/10.3390/horticulturae11091003 (registering DOI) - 23 Aug 2025
Abstract
Little information is available on the yellow-fleshed Zespri Zesy002 kiwifruit dynamic of mineral nutrient uptake and partitioning within organs. The aim of the present experiment was to find nutrient requirements and supply data for a specific nutrient management plan for Zesy002. The trial [...] Read more.
Little information is available on the yellow-fleshed Zespri Zesy002 kiwifruit dynamic of mineral nutrient uptake and partitioning within organs. The aim of the present experiment was to find nutrient requirements and supply data for a specific nutrient management plan for Zesy002. The trial was conducted, for three years, in northern Italy, on a six-year-old kiwifruit orchard of the variety Zespri Zesy002. During the experiment organs were periodically sampled and analyzed for macro- and micronutrient concentration. A yearly nutrient uptake of 175 g N plant−1, 16 g P plant−1, 138 g K plant−1, 235 g Ca plant−1, 48 g Mg plant−1, 17 g S plant−1, 247 mg B plant−1, 673 mg Cu plant−1, 5.20 g Fe plant−1, 473 mg Mn plant−1, and 263 mg Zn plant−1 was calculated, confirming that kiwifruit is a high-nutrient-demanding species. The nutrients found in the tree organs were divided in two factions: removed (not returned into the soil) and recycled (returned into the soil during and at the end of the growing cycle). The two fractions were similar for N, P, K, S, and Mn. The fraction recycled of Ca, Mg, Cu, and Zn was higher than the fraction removed, and the reverse was observed for Fe. These data created the basis for the determination of the correct nutritional plans that take into consideration not only nutrient requirements but also the dynamics of uptake during the season. Full article
(This article belongs to the Special Issue Mineral Nutrition of Plants)
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15 pages, 2133 KiB  
Article
Tree Species Identity Drives Fungal, but Not Bacterial, Soil Community Shifts in Tropical Monoculture Plantations
by Kristin Saltonstall, Erin R. Spear, Martyna A. Glodowska and Jefferson S. Hall
Forests 2025, 16(9), 1366; https://doi.org/10.3390/f16091366 (registering DOI) - 23 Aug 2025
Abstract
Tree plantations can help reverse the negative impacts of deforestation and land degradation worldwide, and soil microbial communities play key roles in tree growth and productivity. We studied microbial communities in the bulk soil of five native species monoculture plantations in the Republic [...] Read more.
Tree plantations can help reverse the negative impacts of deforestation and land degradation worldwide, and soil microbial communities play key roles in tree growth and productivity. We studied microbial communities in the bulk soil of five native species monoculture plantations in the Republic of Panamá to assess how bacteria and fungi were affected by soil chemistry and plant identity after seven years of tree growth. Relative to the other species, Terminalia amazonia accumulated over three times the aboveground biomass and had lower mortality. Soil nutrients, especially phosphorus, were low, and we found no differences in soil chemistry across the five plantation types. Similarly, there was no difference in alpha diversity of the soil microbial communities across plantation types, and the bacterial communities showed no compositional variation or enrichment of any individual taxa. However, soil fungal communities differed in T. amazonia plantations as compared to the others, exhibiting enrichment or absence of specific taxa of arbuscular mycorrhizal fungi and putative phytopathogens. Our results suggest that T. amazonia may associate with certain microbial taxa that help it overcome low nutrient availability in these habitats. Consideration of plant–soil–microbe interactions in restoration efforts may facilitate tree growth and help to promote climate resilient forested areas. Full article
(This article belongs to the Section Forest Soil)
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35 pages, 6554 KiB  
Review
Selenium Compounds and Their Bioactivities: Molecular Mechanisms and Prospects for Functional Food and Therapeutic Applications
by Xue Hou, Zhiyong Wang and Mu Peng
Plants 2025, 14(17), 2622; https://doi.org/10.3390/plants14172622 (registering DOI) - 23 Aug 2025
Abstract
Selenium (Se) is an essential trace element for the human body and plays a vital role in various physiological processes. Plants serve not only as a major dietary source of selenium but also as natural biofactories capable of synthesizing a wide range of [...] Read more.
Selenium (Se) is an essential trace element for the human body and plays a vital role in various physiological processes. Plants serve not only as a major dietary source of selenium but also as natural biofactories capable of synthesizing a wide range of organic selenium compounds. The bioavailability and toxicity of selenium are highly dependent on its chemical form, which can exert varying effects on human physiology. Among these, organic selenium species exhibit higher bioavailability, lower toxicity, and greater structural diversity. In recent years, plant-derived selenium-containing compounds—selenium-enriched proteins, peptides, polysaccharides, polyphenols, and nanoselenium—have garnered increasing scientific attention. Through a systematic search of databases including PubMed, Web of Science, and Scopus, this review provides a comprehensive overview of selenium uptake and transformation in plants, selenium metabolism in humans, and the classification, composition, structural features, and biological activities of plant-derived selenium compounds, thereby providing a theoretical basis for future research on functional foods and nutritional interventions. Full article
(This article belongs to the Topic Nutritional and Phytochemical Composition of Plants)
19 pages, 2830 KiB  
Article
Community Structure Diversity of Endophytic Fungi in Cissampelos pareira from Different Habitats and Their α-Glucosidase Inhibitory Activity
by Jing Yu, Cuiyun Yin, Zhaoyou Deng, Yin Yuan, Deying Tang, Xuanchao Shi, Yihang Li and Lixia Zhang
J. Fungi 2025, 11(9), 615; https://doi.org/10.3390/jof11090615 (registering DOI) - 22 Aug 2025
Abstract
Cissampelos pareira is a medicinal plant with the potential effect of treating diabetes, commonly used by the Dai people in southern Yunnan Province. However, the wild resources of C. pareira are currently scarce. Endophytic fungi are a natural component of medicinal plants, while [...] Read more.
Cissampelos pareira is a medicinal plant with the potential effect of treating diabetes, commonly used by the Dai people in southern Yunnan Province. However, the wild resources of C. pareira are currently scarce. Endophytic fungi are a natural component of medicinal plants, while also serving as important repositories for discovering active natural products. In this study, we focused on 2-year-old C. pareira plants cultivated in potted and non-potted conditions. The community structure of endophytic fungi in the roots, stems, leaves, and flowers of two cultivation methods of C. pareira was investigated by using high-throughput sequencing (HTS) and traditional culture methods. Through HTS, we discover that the richness and diversity of endophytic fungi in C. pareira are associated with its growth environment and plant tissues. The endophytic fungi richness of C. pareira showed significant differences between the two habitats. And significant differences existed in the diversity of root endophytic fungi of C. pareira compared to those in the stems, leaves, and flowers. Additionally, the richness of endophytic fungi in the stems showed significant differences from that in the roots, leaves, and flowers. The results obtained using traditional culture methods revealed 69 endophytic fungi strains, classified into 2 phylum, 4 classes, 11 orders, 23 families, and 69 genera. The fermentation products of the obtained strains were evaluated for in vitro α-glucosidase inhibitory activity, and the results demonstrated that 11 endophytic fungi strains exhibited an inhibition rate exceeding 80%. The above-mentioned study can provide a theoretical basis for a comprehensive understanding of the community composition and diversity of endophytic fungi in C. pareira. Full article
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22 pages, 12018 KiB  
Article
Effects of Winter Green Manure Incorporation on Grain Yield, Nitrogen Uptake, and Nitrogen Use Efficiency in Different Ratoon Rice Varieties
by Qiwen Hou, Pufan Shao, Sheng Chen, Zhangzhen Yang, Zhixiong Yuan, Liusheng Zhong, Ziyuan Zhao, Yu Wang, Cuo Ga, Jiarui Tang, Yaoyun Xu, Yanfu Zeng, Cong Yu, Cheng Huang and Ying Xu
Agriculture 2025, 15(17), 1801; https://doi.org/10.3390/agriculture15171801 - 22 Aug 2025
Abstract
This study evaluated the effects of winter green manure incorporation on grain yield, nitrogen uptake, and use efficiency in ratoon rice production. A two-year field experiment (2019–2021) was conducted using a split-plot design, with main plots comprising three cropping systems: fallow–ratoon rice (FA), [...] Read more.
This study evaluated the effects of winter green manure incorporation on grain yield, nitrogen uptake, and use efficiency in ratoon rice production. A two-year field experiment (2019–2021) was conducted using a split-plot design, with main plots comprising three cropping systems: fallow–ratoon rice (FA), rapeseed–ratoon rice (RA), and milk vetch–ratoon rice (MV). In the RA and MV systems, green manures were incorporated in situ, while subplots featured two ratoon rice varieties (Yliangyou 911, YLY911; Liangyou 6326, LY6326). Compared to FA treatment, RA and MV treatments significantly increased main crop yields by 16.37% and 9.31%, respectively, with corresponding annual total yield improvements of 11.34% and 7.78%. Under RA treatment, LY6326 achieved significantly higher yields than YLY911. Biomass accumulation analysis revealed that RA and MV treatments enhanced plant dry matter by 24.40% and 5.63% at heading stage, and 9.83% and 7.47% at maturity, respectively, relative to FA treatment. Green manure incorporation improved plant nitrogen content at maturity (9.42% and 10.29% for RA and MV, respectively) and panicle nitrogen accumulation (11.73% and 38.26%, respectively) compared to fallow treatment. Nitrogen use efficiency metrics demonstrated that RA and MV treatments enhanced nitrogen harvest index by 1.54% and 5.65%, respectively, while nitrogen partial factor productivity increased by 11.34% and 7.78%. Varietal comparison confirmed that LY6326 exhibited superior nitrogen accumulation and utilization compared to YLY911. These findings demonstrate that winter green manure incorporation significantly enhances grain yield and nitrogen use efficiency in ratoon rice systems, providing a scientific foundation for developing sustainable and productive rice cropping practices. Full article
(This article belongs to the Special Issue Innovative Conservation Cropping Systems and Practices—2nd Edition)
25 pages, 14212 KiB  
Article
Optimization of Composting Locations for Livestock Manure in Bangladesh: Spatial Analysis-Based Potential Environmental Benefits Assessment
by Zinat Mahal, Helmut Yabar and Md Faisal Abedin Khan
Clean Technol. 2025, 7(3), 72; https://doi.org/10.3390/cleantechnol7030072 - 22 Aug 2025
Abstract
For sustainable livestock manure management, composting is a common practice for supplying nutrients to crops. Therefore, optimizing plant locations for composting from livestock manure is essential in Bangladesh. This study performed a land suitability analysis using Geographic Information System (GIS) spatial modeling to [...] Read more.
For sustainable livestock manure management, composting is a common practice for supplying nutrients to crops. Therefore, optimizing plant locations for composting from livestock manure is essential in Bangladesh. This study performed a land suitability analysis using Geographic Information System (GIS) spatial modeling to identify suitable sites for composting plants, which was optimized through network analysis. After spatial analysis, 15, 42, and 147 locations were identified for large-scale, medium-scale, and small-scale manure-based compost production, respectively, across different scenarios. As a result, approximately 1537.74 kilotons/year of compost can be generated from 2703.86 kilotons of livestock manure, replacing about 44.31% of synthetic fertilizer use in Bangladesh in 2024. The potential reduction in greenhouse gas (GHG) emissions was assessed at 1986.76 gigagrams CO2eq/year, with nutrient leaching reduction potentials of 15.11 and 10.98 kilotons/year for nitrogen and phosphorus, respectively. Additionally, around 4.51 million tons of livestock manure can be disposed of annually by establishing composting plants. However, assessing the potential environmental benefits by optimizing composting plant locations can support the development of strategies to produce organic fertilizer by utilizing natural resources in Bangladesh. Full article
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20 pages, 1257 KiB  
Article
Effects of Nitrogen–Phosphorus Co-Application on Biomass Allocation and Accumulation in Two-Year-Old Pinus yunnanensis Seedlings
by Jianzhen Liao, Yaqi Li, Boning Yang, Chiyu Zhou, Zixing Pan, Lin Chen, Nianhui Cai and Yulan Xu
Biology 2025, 14(9), 1115; https://doi.org/10.3390/biology14091115 - 22 Aug 2025
Abstract
Pinus yunnanensis is a significant native tree species in southwestern China, contributing substantially to the area’s ecological stability and economic growth. However, its growth rate tends to be relatively slow during the seedling stage, and fertilization is crucial to promote seedling growth. This [...] Read more.
Pinus yunnanensis is a significant native tree species in southwestern China, contributing substantially to the area’s ecological stability and economic growth. However, its growth rate tends to be relatively slow during the seedling stage, and fertilization is crucial to promote seedling growth. This study used two-year-old P. yunnanensis seedlings as experimental materials and applied a 3 × 3 factorial design, combining three nitrogen (N) levels (0, 0.4, and 0.8 g·plant−1) supplied in the form of urea with three levels of phosphorus (P) (0, 0.8, and 1.6 g·plant−1) supplied in the form of superphosphate to form nine treatments, denoted as T1 to T9. This study was carried out in the open-air nursery of Southwest Forestry University, with fertilization beginning in July and observations continuing until December of the same year. Using allometric growth analysis and constructing the fertilizer response regression equation, we investigated the effects of fertilization on biomass accumulation in P. yunnanensis. The findings revealed that fertilization significantly increased the biomass allocation ratio to roots but decreased the allocation to needles and aboveground parts (p < 0.05). Allometric growth analysis showed that root growth was more rapid than stem and needle growth, and the growth rate of belowground parts exceeded that of aboveground parts. Allometric growth between organs differed among treatments, whereas the allometric growth relationship between aboveground and belowground biomass showed no significant difference across treatments. Moderate N and P fertilizer application promoted biomass accumulation in all organs, with T5 (N: 0.4 g·plant−1; P: 0.8 g·plant−1) exhibiting the highest biomass accumulation. Based on the comprehensive analysis of optimal N and P fertilizer requirements for biomass accumulation across different organs, the recommended fertilizer application rates are as follows: N 0.5–0.6 g·plant−1 and P 0.5–0.9 g·plant−1, with an optimal N:P ratio ranging from 1:0.8 to 1:1.8. The results establish a scientific rationale for enhancing fertilization methods in P. yunnanensis seedling cultivation, contributing to the slow growth issue during the seedling stage. Full article
(This article belongs to the Section Conservation Biology and Biodiversity)
22 pages, 2402 KiB  
Article
Influence of Organic Mulching Strategies on Apple Tree (Mallus domestica BORKH.) Development, Fruit Quality and Soil Enzyme Dynamics
by Ioana Maria Borza, Cristina Adriana Rosan, Daniela Gitea, Manuel Alexandru Gitea, Alina Dora Samuel, Carmen Violeta Iancu, Ioana Larisa Bene, Daniela Padilla-Contreras, Cristian Gabriel Domuta and Simona Ioana Vicas
Agronomy 2025, 15(9), 2021; https://doi.org/10.3390/agronomy15092021 - 22 Aug 2025
Abstract
Mulching is a sustainable agronomic practice that can improve soil quality and fruit characteristics in crops. This study investigated the influence of sheep wool mulch and a soil conditioner on growth, the accumulation of bioactive compounds, and soil enzymatic activity in apple orchards. [...] Read more.
Mulching is a sustainable agronomic practice that can improve soil quality and fruit characteristics in crops. This study investigated the influence of sheep wool mulch and a soil conditioner on growth, the accumulation of bioactive compounds, and soil enzymatic activity in apple orchards. A two-year field experiment (2023–2024) was conducted using three experimental methods: mulching with sheep wool (V2), application of a soil conditioner, corn starch-based polymer (V3), and a combination of sheep wool and corn starch-based polymer (V4) along with a control (V1). Tree growth parameters, fruit physicochemical properties, total phenolic and flavonoid content, and soil enzyme activities (dehydrogenase, catalase, phosphatase) were assessed. Data were analyzed using Principal Component Analysis (PCA) and Pearson’s correlation. PCA showed that the combined variant (V4) improved fruit size, weight, and bioactive compound content, while wool mulch alone (V2) was associated with higher fruit yield and better vegetative growth. Catalase activity correlated positively and consistently with bioactive compounds in both years, while phosphatase activity showed an intensified positive relationship in 2024. Dehydrogenase activity was negatively correlated with phenolic content in both seasons. Organic and integrated mulching practices can beneficially modulate both aboveground and belowground plant–soil interactions. The combined variant proved to be the most effective strategy, enhancing fruit nutritional quality and supporting sustainable apple orchard management. Full article
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25 pages, 2365 KiB  
Article
Decentralized Model for Sustainable Aviation Fuel (SAF) Production from Residual Biomass Gasification in Spain
by Carolina Santamarta Ballesteros, David Bolonio, María-Pilar Martínez-Hernando, David León, Enrique García-Franco and María-Jesús García-Martínez
Resources 2025, 14(9), 133; https://doi.org/10.3390/resources14090133 - 22 Aug 2025
Abstract
Decarbonizing air transport is a major challenge in the global energy transition since electrification is not yet feasible. Sustainable aviation fuel (SAF) is a promising solution because it can reduce CO2 emissions without major infrastructure changes. This study proposes a decentralized model [...] Read more.
Decarbonizing air transport is a major challenge in the global energy transition since electrification is not yet feasible. Sustainable aviation fuel (SAF) is a promising solution because it can reduce CO2 emissions without major infrastructure changes. This study proposes a decentralized model for producing SAF in Spain through the gasification of residual lignocellulosic biomass followed by a refinement process using Fischer–Tropsch (FT) synthesis. The model uses underexploited agricultural residues such as cereal straw, vine pruning, and olive pruning, converting them into syngas in medium-scale facilities situated near biomass sources. The syngas is then transported to a central upgrading unit to produce SAF compliant with ASTM D7566 standards. The following two configurations were evaluated: one with a single gasification plant and upgrading unit and another with three gasification plants supplying one central FT facility. Energy yields, capital and operational expenditures (CAPEX and OPEX), logistic costs, and the levelized cost of fuel (LCOF) were assessed. Under a conservative scenario using one-third of the available certain types of biomass from three regions of Spain, annual SAF production could reach 517.6 million liters, with unit costs ranging from 1.63 to 1.24 EUR/L and up to 47,060 tonnes of CO2 emissions avoided per year. The findings support the model’s technical and economic viability and its alignment with circular economy principles and climate policy goals. This approach offers a scalable and replicable pathway for decarbonizing the aviation sector using local renewable resources. Full article
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15 pages, 3992 KiB  
Article
Characteristics of Organisms and Origin of Organic Matter in Permian Shale in Western Hubei Province, South China
by Yuying Zhang, Baojian Shen, Dongjun Feng, Bo Gao, Pengwei Wang, Min Li, Yifei Li and Yang Liu
Processes 2025, 13(9), 2673; https://doi.org/10.3390/pr13092673 - 22 Aug 2025
Abstract
Permian shale gas is a kind of energy resource with commercial development potential. The characteristics of its organic source and enrichment have received extensive attention in recent years. This study systematically analyzed the variations in types and assemblages of hydrocarbon-forming organisms across different [...] Read more.
Permian shale gas is a kind of energy resource with commercial development potential. The characteristics of its organic source and enrichment have received extensive attention in recent years. This study systematically analyzed the variations in types and assemblages of hydrocarbon-forming organisms across different stratigraphic layers of Permian shale in western Hubei through scanning electron microscopy (SEM) and microscopic observations. Moreover, the source characteristics and enrichment mechanisms of organic matter in Permian shale were identified. Hydrocarbon generation in Permian shale is primarily attributed to planktonic algae-derived acritarchs, supplemented by higher plants and green algae, based on the observation under the SEM and microscope. The hydrocarbon-forming microorganisms in the Gufeng Formation are predominantly characterized by acritarchs. A notable decrease in acritarch content is observed at the bottom of the Wujiaping Formation, accompanied by a significant increase in higher plant constituents and a slight rise in green algae abundance. Subsequently, from the middle-upper members of the Wujiaping Formation through the Dalong Formation, acritarch concentrations rebound while higher plants and green algae contributions diminish. The organic matter in the studied layer is predominantly generated from planktonic algae (acritarchs and green algae), with subordinate contributions from terrestrial higher plants. During the sedimentary stage of the Gufeng Formation, rising sea levels sustained a deep siliceous shelf environment in the E’xi Trough, where organic matter was primarily sourced from acritarchs, with limited terrigenous input. The regressive phase at the bottom of the Wujiaping Formation resulted in coastal marsh throughout the E’xi Trough, creating a mixed organic matter assemblage of aquatic planktonic algae and enhanced terrestrial higher plant material. As sedimentation progressed into the middle-upper Wujiaping Formation and Dalong Formation, the E’xi Trough evolved into a deep siliceous shelf and platform-margin slope environment. During this stage, organic matter was again predominantly supplied by planktonic algae (mainly acritarchs), with reduced terrestrial organic input. These findings provide valuable theoretical insights for guiding Permian shale gas exploration and development strategies. Full article
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17 pages, 2268 KiB  
Article
Brassinosteroids Enhance Low-Temperature Resistance by Promoting the Formation of Sugars in Maize Mesocotyls
by Siqi Sun, Xiaoqiang Zhao, Xin Li and Yining Niu
Plants 2025, 14(17), 2612; https://doi.org/10.3390/plants14172612 - 22 Aug 2025
Abstract
The germination and elongation of maize in the early growth stage are closely related to the elongation of the mesocotyl, which is one of the earlier parts that are able to sense external temperature, except for the coleoptile. And, low-temperature (LT) stress can [...] Read more.
The germination and elongation of maize in the early growth stage are closely related to the elongation of the mesocotyl, which is one of the earlier parts that are able to sense external temperature, except for the coleoptile. And, low-temperature (LT) stress can significantly influence the survival and growth of maize seedlings. In addition, the brassinosteroids (BRs) have also been applied to alleviate the damage suffered by various plants in LT in recent years. However, the interaction relationship among LT, BRs, and sugar remains unclear. Therefore, we examined the changing relationships among the contents of glucose, sucrose, and starch, as well as the changes in differentially expressed genes (DEGs) in the starch and sucrose metabolism and glycolysis/gluconeogenesis pathways. Herein, compared to CK (0 μM 24-epibrassinolide (EBR) application at 25 °C), the contents of glucose and sucrose all increased by 0.26, 0.47, and 0.70 mg g−1 FW and 0.80, 0.30, and 0.61 mg g−1 FW, respectively, under the CKE (2.0 μM 24-epibrassinolide (EBR) application at 25 °C), LT (0 μM 24-epibrassinolide (EBR) application at 10 °C), and LTE (2.0 μM 24-epibrassinolide (EBR) application at 10 °C) treatments, but the contents of starch decreased under LT and LTE treatments by −0.54% and −0.20%, compared to CK. This suggested that not only did the sugar signaling and metabolism play key roles in regulating LT tolerance but the application of EBR can also alleviate the damage caused by LT by regulating the sugar accumulation level. Meanwhile, 108 DEGs in the starch and sucrose metabolism pathway and 65 DEGs in the glycolysis pathway were identified at the transcriptome level. The common Zm00001d042146 in both pathways is always down-regulated, and the down-regulation multiple when EBR is added is less than the LT. In addition, key genes such as Zm00001d021598, Zm00001d034017, and Zm00001d029091, were all differentially expressed under LT, and the expression multiples decreased when EBR was added. In conclusion, our results provide new insights into the molecular mechanism by which exogenous application of EBR enhances the low-temperature tolerance of maize seedlings. The germination and elongation of maize in the early growth stage are closely related to the elongation of the mesocotyl, which is one of the first parts to sense external temperature, aside from the coleoptile. Low-temperature (LT, 10~15 °C) stress can significantly affect the survival and growth of maize seedlings. Additionally, brassinosteroids (BRs) have been used in recent years to help alleviate damage caused by LT in various plants. However, the interaction among LT, BRs, and sugar remains unclear. Therefore, we examined the relationships among the contents of glucose, sucrose, and starch, along with the changes in differentially expressed genes (DEGs) involved in starch and sucrose metabolism and glycolysis/gluconeogenesis pathways. Compared to CK (0 μM 24-epibrassinolide (EBR) application at 25 °C), the contents of glucose and sucrose increased by 0.26, 0.47, and 0.70 mg g−1 FW and 0.80, 0.30, and 0.61 mg g−1 FW, respectively, under the CKE (2.0 μM 24-epibrassinolide (EBR) application at 25 °C), LT (0 μM 24-epibrassinolide (EBR) application at 10 °C), and LTE (2.0 μM 24-epibrassinolide (EBR) application at 10 °C) treatments. However, starch contents decreased under LT and LTE treatments, by −20.54% and −0.20%, respectively, compared to CK. This suggests that sugar signaling and metabolism play key roles in regulating LT tolerance, and the application of EBR may alleviate LT damage by regulating sugar accumulation levels. Furthermore, 108 DEGs were identified in the starch and sucrose metabolism pathways, along with 23 in glycolysis, with 65 DEGs at the transcriptome level. The common Zm00001d042146 (hexokinase-3) in both pathways is usually down-regulated, and the degree of down-regulation when EBR is added is less than under LT alone. Additionally, key genes such as Zm00001d021598 (glucan endo-1,3-beta-glucosidase 3), Zm00001d034017 (uncharacterized LOC541703), and Zm00001d029091 (sucrose synthase 2) were differentially expressed under LT, with their expression levels decreasing further when EBR was added. In conclusion, our results provide a new direction into the molecular mechanisms by which exogenous EBR application enhances low-temperature tolerance in maize seedlings. Full article
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