Search Results (27)

As one of the busiest airports in East China, effective bird-strike prevention is of paramount importance for Hangzhou Xiaoshan International Airport. Ground-dwelling insects in airport grasslands serve as a critical food source for insectivorous birds, making the study of insect communities essential for understanding bird activity patterns and mitigating bird-strike risks from a food chain perspective. This study investigates the communities of insects, birds, and vegetation in the flight zone and clear zone of Hangzhou Xiaoshan International Airport. Based on monthly surveys conducted from January to December 2024, we analyzed insect community composition and diversity, assessed bird-strike risks, and examined correlations between insect and bird communities. The results recorded a total of 7744 birds belonging to 107 species, 43 families, and 15 orders in the flight zone and clear zone. Passeriformes was the most species-rich order, and resident birds dominated the avian community. Bird species richness and abundance peaked in spring and autumn. In the flight zone, 18 bird species (e.g., Hirundo rustica) were classified as highly hazardous (R ≥ 15, where R is the calculated risk index) or above. The vegetation survey identified Cynodon dactylon as the dominant plant species in the flight zone. Importantly, positive trends were observed between insectivorous birds and insect abundance, though correlations did not reach statistical significance. To reduce food availability for birds, we recommend stringent management of the grassland habitat in the flight zone, including targeted insect control measures. Given the airport’s location along the East Asian-Australasian Flyway, enhanced bird dispersal efforts should be implemented during peak migration seasons. This study provides a crucial ecological foundation for developing an integrated “vegetation–insect–bird” management strategy for bird-strike prevention at Hangzhou Xiaoshan International Airport and similar aviation hubs. Full article

The global expansion of tree plantations has led to extensive fragmentation of natural forests, posing significant challenges for biodiversity conservation. Understanding the diversity patterns and underlying mechanisms of ground-dwelling insects in these fragmented landscapes is critical to inform effective conservation strategies. To address this, we sampled ground-dwelling insects using pitfall traps across nine remnant natural forest fragments (“islands”) embedded within a tree plantation matrix in Guangxi, China. We examined insect family-level diversity and community composition in relation to fragment isolation (low vs. high) and size (large vs. small) and explored the mechanisms driving the observed patterns. Our results revealed no significant difference in ground-dwelling insect diversity between low-isolation and high-isolation fragments. However, diversity was significantly lower in smaller fragments compared to larger ones. This reduction was primarily driven by decreased seedling density within smaller fragments, directly reflecting the adverse effects of plantation-driven fragmentation on native seedling establishment. Furthermore, we observed noble shifts in community composition of ground-dwelling insects along both fragment isolation and size gradients. Highly isolated fragments exhibited a decline in phytophagous insects and omnivores (with detritivore-herbivore diets), but an increase in detritivores. Smaller fragments exhibited consistent declines across multiple insect taxa spanning various dietary guilds. The observed changes in ground-dwelling insect composition were driven by shifts in plant (especially seedling) community composition. Our findings reveal a clear cascading effect: plantation-driven fragmentation limits native plant regeneration, and these limitations subsequently propagate to higher trophic levels, profoundly impacting ground-dwelling insects. Effective restoration of plantation-fragmented landscapes requires strategies that both prioritize the preservation of large, continuous forest fragments and promote native seedling recruitment within existing fragments. Full article

Masson pine (Pinus massoniana Lamb.) is the most widely planted tree species in southern China, playing a critical role in forestry production and reforestation. Understanding the contribution of Masson pine plantations to biodiversity conservation is essential for sustainable land-use policies. We conducted comparative studies to examine the family diversity and composition of ground-dwelling and aerial insects in Masson pine plantations and adjacent natural forests at regional (spanning five forest types across Guangxi, China) and local (at Yachang, Guangxi) scales. We investigated the mechanisms driving the differences in insect community assemblages between the two forest types at the local scale. Our results indicated that aerial insect diversity and composition in Masson pine plantations were comparable to those in natural forests. However, ground-dwelling insects in plantations showed a significant decline in diversity and a notable shift in community composition, with a decrease in highly mobile omnivores (e.g., Drosophilidae and Nitidulidae) and an increase in crawling detritivores (e.g., Blattidae and Gryllidae). These patterns were consistent at both regional and local scales. At the local scale, the shift in ground-dwelling insect community composition was linked to decreased understory tree density (explaining 45.9% of the compositional variation), reduced litter Ca content (29.7%), and increased litter cover (13.5%) in plantations. To enhance ground-dwelling insect diversity in Masson pine plantations, mixed planting with broad-leaved species offers an effective management strategy. This approach both enriches litter nutrients and reduces needle litter accumulation, thereby supporting the recovery of understory vegetation. Full article

The invasive sap-feeding pest Toumeyella parvicornis (pine tortoise scale) is rapidly spreading across Europe, threatening pine ecosystems, particularly in forest–urban areas of Italy. In this scenario, early detection and monitoring strategies are critical to prevent new outbreaks and mitigate impacts in infested regions. Current surveillance is challenged by the lack of rapid, sensitive tools for indirect detection of this cryptic, canopy-dwelling pest, despite advancements in molecular diagnostics and environmental DNA (eDNA). Here, we established a highly specific qPCR assay using LNA probe chemistry for detecting T. parvicornis DNA from both adult insects and their excreted honeydew. DNA was successfully isolated/quantified from all tested matrices. We recorded average Cq values of 20.9 for insect specimens and 30.3 for collected honeydew samples. Targeting the COI barcoding region, the assay demonstrated excellent specificity in both in silico and in vitro tests, showing no cross-reactivity to other pine-associated taxa. The limit of detection for DNA isolated from insect was 64 fg/µL. This is the first diagnostic protocol to use honeydew as a matrix for indirect detection of T. parvicornis. Optimized for routine application by Plant Health Services, this eDNA-based tool offers a valuable approach for future monitoring of sap-sucking hemipterans in multiple environments. Full article

Wild bees are vital for the pollination of native plants and crops, providing essential ecosystem services. Climate change is known to impact biodiversity and species distributions, but insects adapted to desert ecosystems may exhibit unique physiological, behavioral, and evolutionary responses. The desert pallid bee (C. pallida), a solitary bee native to the arid southwestern United States and northern Mexico, primarily forages on yellow palo verde (P. microphylla), blue palo verde (P. florida), and desert ironwood (O. tesota). This study used MaxEnt to estimate the current and projected geographical overlap of suitable habitats for C. pallida and its host plants. Here, we used MaxEnt to estimate the current and forecasted overlapping geographically suitable habitat of C. pallida with all three host plants. We forecasted potential environmentally suitable areas for each species to the year 2040 using the current distribution model and climate projections with moderate CO₂ levels. We found a continued spatial alignment in the suitable area of the bee and its host plants with a 70% increase in the range overlap area, though shifted to higher average altitudes and a slight northern expansion. These findings may provide insight to stakeholders on the conservation needs of desert-dwelling pollinators. Full article

Structurally well-defined compounds have advantages for quality control in plant biostimulant production and application processes. Humic acid (HA) is a biostimulant that significantly affects plant growth, and soil-dwelling Protaetia brevitarsis larva (PBLs) can rapidly convert agricultural waste into HA. In this study, we use PBLs as a model to investigate HA formation and screen for structurally well-defined HA-related plant biostimulant compounds. Dephasing magic angle spinning nuclear magnetic resonance (¹³C DD–MAS NMR) analysis indicated HA structural changes during PBL digestion; metabolic profiling detected seven HA-related aromatic ring-containing compounds. A total of six compounds that significantly stimulate plant growth were identified through plant experiments, and all six compounds demonstrate the ability to enhance seed germination. It is noteworthy that piperic acid exhibits a remarkable promotion of root growth in plants, a finding reported for the first time in this study. Thus, this study not only provides insights into the insect-mediated transformation of HA but also illustrates a new method for discovering structurally well-defined plant biostimulant compounds. Full article

Entomopathogenic nematodes (EPNs), small soil-dwelling non-segmented roundworms, are obligate parasites of insects and commonly used in agriculture for biological control of insect pests. For successful reproduction, EPNs must identify, move towards, and successfully infect a suitable insect host in a chemically complex soil environment. EPNs can have innate host insect preferences and can be attracted to semiochemicals associated with that host. They can also develop strong learned preferences for chemical signals associated with the presence of a host, such as herbivory-induced volatiles. We hypothesized that simultaneous manipulation of innate and learned preferences could result in increased biological control services of EPNs in agriculture. Separate cohorts of the EPN Steinernema diaprepesi were raised on two insect hosts, Galleria mellonella and Tenebrio molitor, for multiple generations until the nematodes in a dual-choice olfactometer exhibited preference for the host they were reared on. Subsequently, the two strains of nematodes were imprinted on three plant-produced terpenoids of agricultural significance: pregeijerene, β-caryophyllene, and α-pinene. After exposure to one of the plant compounds, the behavior of the EPNs was assayed in an olfactometer where the two host insects were presented with and without the plant compounds. We found that plant volatile exposure increased the infection rate of the nematodes, and some host–compound combinations proved to be attractive, but other combinations appeared to become repellent. These results indicate that learned preference is neither subordinate nor superior to innate preference, and that infection efficiency can vary with compound exposure and insect host. Full article

Entomopathogenic fungi Metarhizium species are generally employed to manage the soil-dwelling stage of insect pests, and are known for their rhizocompetency property. Since this fungus is typically recommended for use in soil, it could potentially be investigated as a bioinoculant to reduce abiotic stress, such as salinity, along with improved plant growth promotion. Salt stress tolerance potential of native Metarhizium isolates was evaluated based on mycelial fresh weight, dry weight, and spore yield. All the isolates were found to tolerate NaCl concentrations (50 mM, 100 mM, 150 mM, 200 mM, 250 mM, and 300 mM) supplemented in the culture medium. Metarhizium anisopliae (AAUBC-M15) and Metarhizium pinghaense (AAUBC-M26) were found to be effective at tolerating NaCl stress up to 200 mM NaCl. These two isolates were analyzed in vitro for plant growth-promoting traits at elevated salt concentrations (100 and 200 mM NaCl). No significant effect on IAA production was reported with the isolate M. pinghaense (AAUBC-M26) (39.16 µg/mL) or in combination with isolate M. anisopliae (AAUBC-M15) (40.17 µg/mL) at 100 mM NaCl (38.55 µg/mL). The salinity stress of 100 mM and 200 mM NaCl had a significant influence on the phosphate solubilization activity, except in the co-inoculation treatment at 100 mM NaCl. The isolates were positive for ACC deaminase enzyme activity. An increase in salt concentration was accompanied by a steady and significant increase in chitinase enzyme activity. Total phenolics (149.3 µg/mL) and flavonoids (79.20 µg/mL) were significantly higher in the culture filtrate of Metarhizium isolates at 100 mM NaCl, and gradual decline was documented at 200 mM NaCl. M. pinghaense (AAUBC-M26) proved to be promising in reducing the salt stress in tomato seedlings during the nursery stage. In the pot culture experiment, the treatment comprising soil application + seedling root dip + foliar spray resulted in improved growth parameters of the tomato plant under salt stress. This study shows that Metarhizium, a fungus well known for controlling biotic stress brought on by insect pests, can also help plants cope with abiotic stress, such as salinity. Full article

Epigeic generalist predators play a crucial role in terrestrial ecosystems, connecting aboveground and belowground food webs. Using stable isotope compositions (δ¹³C and δ¹⁵N values), we assessed the trophic niches of the two main groups of generalist predators (ground beetles (Coleoptera, Carabidae) and spiders (Arachnida: Aranei)), as well as their potential prey (phytophagous and saprophagous insects), plants, and soils in the forest, steppe, and transitional ecosystems located in the forest–steppe of southwestern Siberia. We hypothesized that (1) the trophic niche of carabids is wider than that of spiders, because some ground beetles are omnivorous, and (2) the contribution of invertebrates from the detrital food web (saprophages) to the diet of generalist predators is higher in the “detrital” forest ecosystem than in the steppe, which should be reflected in increased δ¹³C and δ¹⁵N values of the predators in the forest. In total, 16 species of carabid and 17 species of spider were analyzed. The δ¹⁵N values of ground beetles suggested a wide range of trophic niches corresponding to two or three trophic levels. Omnivorous carabids of the genera Amara and Harpalus had the minimum δ¹⁵N values. The carbon isotope compositions of the ground beetles suggests that most predatory species were predominantly involved in grazing food chains. Spiders had on average increased δ¹⁵N values compared to ground beetles, and a relatively narrow range of δ¹⁵N values. The isotopic niche occupied by spiders hardly overlapped with the isotopic niche of carabids, which may indicate a significant difference in the range of consumed resources. Thus, our data suggest a significant difference in the trophic niches of the key generalist predators, ground beetles and spiders, which was observed both in the forest and the steppe habitats. Spiders appear to be more closely associated with detrital food webs than ground beetles. Contrary to our expectations, we did not find higher δ¹³C and δ¹⁵N values in ground-dwelling generalist predators in the forest compared to the steppe ecosystem. Full article

Entomopathogenic fungi (EPF) are cosmopolitan species of great interest in pest management due to their ability to cause epizooty in soil-dwelling and aboveground insects. Besides their direct effect against a wide host range of serious agricultural insect pests, such as aphids, a major emphasis has been placed on investigating the impact of EPF with endophytic traits (EIPF) on aboveground tripartite interactions between host plants, herbivores and beneficial insects. However, despite their valuable role in biocontrol processes, there is still more to explore about their diverse potential as ecofriendly biological control agents. Herein, we provide an overview of the meaningful role and faced challenges following the use of EPF and EIPF to control aphids. Full article

Exhibiting manifold ecological impacts on terrestrial biota, roads have become a major driver of environmental change nowadays. However, many insect groups with high indication potential, such as grasshoppers and crickets (Orthoptera), have been largely neglected in road ecology research from a functional perspective. Using two complementary sampling methods, we have investigated the spatial dynamics of functional diversity and six functional traits in orthopteran assemblages, with respect to motorway proximity and the associated environmental factors, in a grassland habitat in the Lika region, Croatia. This research shows, for the first time, that road proximity can facilitate an increase in the functional diversity of orthopteran assemblages, with shifts in functional traits related to mobility, feeding habits and lifestyle being primarily driven by changes in vegetation height. Our findings also suggest that our ability to detect road-related patterns depends on the choice of a diversity measure and sampling method, since different components of orthopteran assemblages (plant-dwelling vs. ground-dwelling) exhibit different functional responses to road proximity. Full article

Entomopathogenic fungi are a special group of soil-dwelling microorganisms that infects and kills insects and other arthropods through cuticle penetration. They are currently used as biocontrol agents against insect plant pests and play a vital role in their management. Regardless that entomopathogenic fungi are currently on the agriculture market, their full potential has not yet been utterly explored. Up to date substantial research has covered the topic revealing numerous uses in pest management but also on their ability as endophytes, assisting the plant host on growth and pathogen resistance. This article addresses the literature on entomopathogenic fungi through the years, noting their mode of action, advantages, potential applications, and prospects. Full article

Plants influenced the evolution of plant-dwelling stink bugs’ systems underlying communication with chemical and substrate-borne vibratory signals. Plant volatiles provides cues that increase attractiveness or interfere with the probability of finding a mate in the field. Mechanical properties of herbaceous hosts and associated plants alter the frequency, amplitude, and temporal characteristics of stink bug species and sex-specific vibratory signals. The specificity of pheromone odor tuning has evolved through highly specific odorant receptors located within the receptor membrane. The narrow-band low-frequency characteristics of the signals produced by abdomen vibration and the frequency tuning of the highly sensitive subgenual organ vibration receptors match with filtering properties of the plants enabling optimized communication. A range of less sensitive mechanoreceptors, tuned to lower vibration frequencies, detect signals produced by other mechanisms used at less species-specific levels of communication in a plant environment. Whereas the encoding of frequency-intensity and temporal parameters of stink bug vibratory signals is relatively well investigated at low levels of processing in the ventral nerve cord, processing of this information and its integration with other modalities at higher neuronal levels still needs research attention. Full article

Plant vascular systems can translocate the entomopathogen Bacillus thuringiensis from the soil into plant tissues. However, whether other soil dwelling entomopathogens utilize plant vascular tissue for movement has not yet been fully explored. We used Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) to evaluate whether baculoviruses, a common entomopathogen and bioinsecticide, can be transported through the plant vascular pathways of Zea mays. We found that our treatments did not allow a sufficient virus translocation into the plant to induce a lethal infection in insects, which was confirmed by a molecular analysis. While other entomopathogens translocate, baculoviruses may not be one of them. Full article

Landscape diversification with flowering plants can benefit pollinators and natural enemies, although insect pests can also use floral resources for nutrition and chemoprotection. Corn rootworms (Coleoptera: Chrysomelidae, Diabrotica spp.) are major pests of corn (Zea mays L.), and while subterranean larvae primarily feed on corn roots, adult rootworms commonly consume floral resources from other plant species. We quantified the species, density, and sex of adult corn Diabroticite rootworm beetles on wild and cultivated sunflower, corn, and squash, quantified pollen within the bodies of adult northern corn rootworms [NCR, D. barberi (Smith & Lawrence)], and investigated how consumption of sunflower and corn pollen by NCR adults impacted predation of their eggs by two soil-dwelling mites with different feeding specialization. NCR were the most common Diabroticite species on sunflower inflorescences and western corn rootworm (WCR, D. v. virgifera LeConte) were more abundant in corn and squash blossoms. Pollen feeding by NCR adults did not impact egg predation by omnivorous Tyrophagus putrescentiae (Schrank) (Acari: Sarcoptiformes, Acaridae), but predatory Stratiolaelaps scimitus (Womersley) (Acari: Mesostigmata, Laelapidae) ate eggs less frequently and took longer to feed on eggs from NCR females that had fed on sunflower pollen. This research suggests pollen feeding by adult NCR can impact predation of their eggs. While increasing plant diversity can benefit natural enemies and pest control within agroecosystems, it is important to consider how floral resources alter dietary preferences of biocontrol agents. Full article