Search Results (423)

Clematis L., a significant genus of climbing plants within the Ranunculaceae family, boasts widespread germplasm resources distributed across temperate to tropical regions globally, with Asia preserving particularly abundant native populations. This review systematically summarizes recent advances in Clematis research: in terms of physiological characteristics, the research focuses on the evolution of plant classification, chromosomal evolutionary features revealed by karyotype analysis, and studies on genetic diversity and phylogenetic relationships based on molecular markers; in breeding methods, it summarizes the two major technical systems of sexual and asexual reproduction; regarding ornamental traits, it emphasizes the molecular mechanisms of flower color and form development, and synthesizes breakthroughs in techniques for flowering period regulation and research on the biosynthesis pathways of floral scent metabolites; in the field of stress resistance mechanisms, it thoroughly examines physiological responses and molecular adaptation mechanisms under abiotic stresses such as UV radiation, drought, high temperature, and intense light, and outlines research progress on pathogen types of major diseases; in studies of medicinal value, it highlights the material basis and mechanisms of pharmacological activities including anti-inflammatory, analgesic, and antitumor effects. Through multidimensional comprehensive analysis, this review aims to elucidate the comprehensive development potential of Clematis, providing theoretical foundations and practical guidance for germplasm resource innovation, breeding of high-ornamental-value cultivars, and stress resistance applications. Full article

The Cutting Development Chamber (CDC) design is presented as an innovative solution to crucial human challenges, such as food and plant medicinal production. Unlike conventional propagation chambers, the CDC is a much more comprehensive research tool, specifically designed to optimize plant reproduction from cuttings. It maintains precise control over humidity, temperature, and lighting, which are essential parameters for plant development, thus maximizing the success rate, even in difficult-to-propagate species. Its modular design is one of its main strengths, allowing users to adapt the chamber to their specific needs, whether for research studies or for larger-scale propagation. The most distinctive feature of this chamber is its ability to collect detailed, labeled data, such as images of plant growth and environmental parameters that can be used in artificial intelligence tasks, which differentiate it from chambers that are solely used for propagation. A study that validated and calibrated the chamber design using cuttings of various species demonstrated its effectiveness through descriptive statistics, confirming that CDC is a powerful tool for research and optimization of plant growth. In validation experiments (Aloysia citrodora and Stevia rebaudiana), the system generated 6579 labeled images and 67,919 environmental records, providing a robust dataset that confirmed stable control of temperature and humidity while documenting cutting development. Full article

Heavy metal and metalloid stress, particularly from toxic elements like cadmium (Cd), poses a growing threat to plant ecosystems, crop productivity, and global food security. Elevated concentrations of these contaminants can trigger cytotoxic and genotoxic effects in plants, severely impairing growth, development, and reproduction. In recent years, epigenetic mechanisms have emerged as crucial regulators of plant responses to heavy metal stress, offering novel insights and strategies for enhancing plant resilience in contaminated environments. This review synthesises current advances in the field of plant epigenetics, focusing on key modifications such as DNA methylation, histone acetylation and remodelling, chromatin dynamics, and small RNA-mediated regulation. These processes not only influence gene expression under metal-induced stress but also hold promise for long-term adaptation through transgenerational epigenetic memory. Recent developments in high-throughput sequencing and functional genomics have accelerated the identification of epigenetic markers associated with stress tolerance, enabling the integration of these markers into breeding programs and targeted epigenome editing strategies. Special attention is given to cadmium stress responses, where specific epigenetic traits have been linked to enhanced tolerance. As plant epigenomic research progresses, its application in sustainable agriculture becomes increasingly evident offering environmentally friendly solutions to mitigate the impact of heavy metal pollution. This review provides a foundation for future research aimed at leveraging epigenetic tools to engineer crops capable of thriving under metal stress, thereby contributing to resilient agricultural systems and sustainable food production. Full article

The rising global demand for high-quality animal protein has driven the development of advanced technologies in high-density livestock farming. Rabbits, with their rapid growth, high reproductive efficiency, and excellent feed conversion, play an important role in modern animal agriculture. However, large-scale rabbit farming poses challenges in timely health inspection and environmental monitoring. Traditional manual inspections are labor-intensive, prone-to-error, and inefficient for real-time management. To address these issues, we propose Livestock Environmental Monitoring System–Gantry (LIVEMOS-G), an intelligent gantry-based monitoring system tailored for large-scale rabbit farms. Inspired by plant phenotyping platforms, the system integrates a three-axis motion module with multi-source imaging (RGB, depth, near-infrared, thermal infrared) and an environmental sensing module. It autonomously inspects around the farm, capturing multi-angle, high-resolution images and real-time environmental data without disturbing the rabbits. Key environmental parameters are collected accurately and compared with welfare standards. After training on an original dataset, which contains a total of 2325 sets of images (each set includes RGB, NIR, TIR, and depth image), the system is able to detect dead rabbits using a fusion-based object detection model during inspections. LIVEMOS-G offers a scalable, non-intrusive solution for intelligent livestock inspection, contributing to enhanced biosecurity, animal welfare, and data-driven management in high-density, modern rabbit farms. It also shows the potential to be extended to other species, contributing to the sustainable development of the animal farming industry as a whole. Full article

The federally threatened Neches River rose-mallow (Hibiscus dasycalyx) is a rare wetland species restricted to Eastern Texas, where population recovery depends on successful pollination and reproduction. The reproductive ecology was investigated to evaluate how floral phenology, pollinator activity, and breeding system influence reproductive success in natural versus created populations. Observations and controlled breeding system trials were conducted during 2024–2025 in a natural population (Houston County) and a created population (Walker County). Flowering was extended and synchronous in the natural population but shorter and less synchronized in the restored population. Breeding system trials confirmed self-compatibility without autogamy: fruit set was highest in outcrossed flowers, moderate in hand-selfed flowers, and absent in bagged flowers. Seed set, seed weight, and germination did not differ significantly between treatments. Pollinator visitation was high in the natural population, dominated by the specialist bee Ptilothrix bombiformis (>98% of visits), resulting in high fruit set. In contrast, low visitation in the restored population was associated with reduced fruit production. Pollen receipt varied significantly between sites and was strongly associated with P. bombiformis activity. These results demonstrate that the successful restoration of H. dasycalyx requires not only suitable habitat and planting efforts but also the presence of effective pollinators to ensure reproductive success. Full article

Oxidative stress (OS), defined as a disturbance in the balance between the production and elimination of reactive oxygen species (ROS), has been widely recognized as a key factor in the pathogenesis of various aquatic animal diseases. With the intensification of aquaculture and increasing environmental pressure, aquatic animals are frequently subjected to stressors that trigger oxidative stress, thereby compromising their health and productivity. This review comprehensively summarizes recent advances in understanding the involvement of oxidative stress in multiple organ-related diseases in farmed aquatic animals, including hepatic/pancreatic injuries, gill lesions, muscle degeneration, skin and shell disorders, metabolic disruptions, immunosuppression, and reproductive impairments. The underlying mechanisms involve excessive ROS-induced lipid peroxidation, inflammation, mitochondrial dysfunction, and the disruption of critical signaling pathways. Additionally, recent advances in nutritional antioxidants (e.g., vitamins, plant extracts), environmental regulation, and feed additives for mitigating oxidative damage are also discussed. A comprehensive understanding of the pathogenesis and regulation of oxidative stress is essential for improving aquatic animal health and enhancing the sustainability of aquaculture systems. Full article

Polycystic ovary syndrome (PCOS) represents one of the most prevalent endocrine–metabolic disorder in women of reproductive age, which includes but not restricted to reproductive disruptions, insulin resistance (IR), hyperandrogenism, and chronic low-grade inflammation. Its heterogeneous pathophysiology arises from the interplay of metabolic, endocrine, and immune factors, including dysregulated adipokine secretion, cytokine-mediated inflammation, oxidative stress (OS), and mitochondrial dysfunction. Current pharmacological therapies, such as metformin, clomiphene, and oral contraceptives, often provide partial benefits and are limited by side effects, necessitating the exploration of safer, multi-target strategies. Flavonoids, a structurally diverse class of plant-derived polyphenols, have gained attention as promising therapeutic candidates in PCOS due to their antioxidant, anti-inflammatory, insulin-sensitizing, and hormone-modulating properties. Preclinical studies in rodent PCOS models consistently demonstrate improvements in insulin sensitivity, normalization of ovarian morphology, restoration of ovulation, and reduction in hyperandrogenism. Human clinical studies, though limited in scale and heterogeneity, report favorable effects of flavonoids such as quercetin, isoflavones, and catechins on glucose metabolism, adipokine balance, inflammatory markers, and reproductive functions. This evidence-based study critically synthesizes mechanistic insights into how flavonoids modulate insulin signaling, adipokine–cytokine crosstalk, OS, and androgen excess, while highlighting translational evidence and emerging delivery systems aimed at overcoming bioavailability barriers. Collectively, flavonoids represent a promising class of nutraceuticals and adjuncts to conventional therapies, offering an integrative strategy for the management of PCOS. Full article

Sphagneticola trilobata (L.) Pruski has been introduced into many countries due to its ornamental and economic value. However, it has been listed in the world’s 100 worst alien invasive species due to its invasive nature. This species easily escapes cultivation and forms dense ground cover. It reproduces asexually through ramet formation from stem fragments. It also produces a large number of viable seeds that establish extensive seed banks. The movement of stem fragments and the dispersal of seeds, coupled with human activity, contribute to its short- and long-distance distribution. S. trilobata grows rapidly due to its high nutrient absorption and photosynthetic abilities. It exhibits high genetic and epigenetic variation. It can adapt to different habitats and tolerate various adverse environmental conditions, including cold and high temperatures, low and high light irradiation, low nutrient levels, waterlogging, drought, salinity, and global warming. S. trilobata has powerful defense systems against herbivory and pathogen infection. These systems activate the jasmonic acid signaling pathway, producing several defensive compounds. This species may also acquire more resources through allelopathy, which suppresses the germination and growth of neighboring plants. These life history traits and defensive abilities likely contribute to its invasive nature. This is the first review to focus on the mechanisms of its invasiveness in terms of growth and reproduction, as well as its ability to adapt to different environmental conditions and defend itself. Full article

Background: The ubiquitin–proteasome system plays a critical role in plant antiviral defense, with HECT-type E3 ubiquitin ligases serving as key regulators of protein turnover. To explore the potential involvement of the HECT gene family in host resistance against tomato yellow leaf curl virus (TYLCV), a comprehensive analysis was conducted in Nicotiana benthamiana. Methods: In this study, the HECT gene family in N. benthamiana was systematically investigated using a genome-wide bioinformatic analysis. The potential roles of these genes in the response to TYLCV infection were further examined using a virus-induced gene silencing (VIGS) technique. Results: Using a Hidden Markov Model approach, 18 NbHECT genes were identified that phylogenetically clustered into four subfamilies with distinct structural features. Chromosomal location and synteny analyses indicated that these genes were unevenly distributed across 11 chromosomes, with 10 instances of segmental duplication identified. Tissue-specific expression profiling demonstrated that 17 NbHECTs were constitutively expressed, with Group III members showing the highest expression in reproductive tissues. Following TYLCV infection, NbHECT6 was significantly downregulated while NbHECT13 was upregulated in both inoculated and systemic leaves. Functional validation through the VIGS approach revealed that suppression of NbHECT6 and NbHECT13 increased host susceptibility, as evidenced by exacerbated symptom severity and enhanced viral DNA accumulation compared to controls. Conclusions: These findings establish NbHECT6 and NbHECT13 as critical components of the plant antiviral response, providing new insights into ubiquitin-mediated defense mechanisms against geminiviruses. Full article

The transition from outcrossing to self-pollination is an evolutionary process in angiosperms. However, the changes in floral volatile composition during this process and their impacts on the behavior of pollinators are poorly understood. Therefore, this study investigated the potential differences in the floral volatile profiles and pollinator attraction capabilities of three invasive Bidens species. The results indicated that Bidens pilosa var. radiata (BH), which serves as a transitional species between facultative outcrossing and obligate outcrossing attracts a greater diversity and abundance of pollinators such as Apis cerana compared to the more self-compatible Bidens frondosa (DL) and Bidens pilosa var. pilosa (SY). Furthermore, a total of 37, 33, and 34 Volatile Organic Compounds (VOCs) were identified in the floral volatiles of BH, DL, and SY, respectively, with no discernible trend of decreased number of floral VOCs owing to increased self-pollination ability. Moreover, eleven significantly different compounds in the floral volatiles of the three Bidens species were obtained. Among these (E)-β-Ocimene (18.31 ± 1.10%) and (Z)-β-Ocimene (33.93 ± 3.49%) in the floral volatiles of BH (52.24 ± 4.59%) was significantly higher than that of DL (1.72 ± 0.50%) and SY (0.32 ± 0.19%). Additionally, Y-tube olfactometer behavioral assays indicated that (E)- and (Z)-β-Ocimene significantly attracted A. cerana. These findings suggested that (E)- and (Z)-β-Ocimene contribute to the attractiveness of BH to local pollinators. Furthermore, it can be inferred that within Bidens, stronger self-pollination ability reduces the relative content of VOCs—such as (E)- and (Z)-β-Ocimene—used to attract generalist pollinators. Full article

The adaptability of several Italian olive cultivars to high-density cultivation was evaluated from 2020 to 2024 in central Italy by assessing their agronomic behavior, with the aim of identifying which Italian olive cultivars can combine high productivity and suitability for intensive mechanization—through high- and very high-density planting systems—allowing biodiversity valorization. The cultivars were Borgiona, Don Carlo, FS17, Gentile di Anghiari, Gentile di Montone, Giulia, Leccio del Corno, Maurino, Moraiolo, Pendolino, Piantone di Falerone, and Piantone di Mogliano. The international cultivar Arbequina was used as a reference. The olive orchard was planted in 2015, at a tree spacing of 5 m × 2 m (1000 trees/ha). Arbequina was found to have limited vigor and high production efficiency, as reported in other works, therefore confirming its suitability for high-density and super-high-density cultivation. Some cultivars, such as Leccio del Corno, Maurino, FS17, Piantone di Mogliano, and Piantone di Falerone, had a production and yield efficiency that was not different from or even higher than Arbequina. Other cultivars found to be promising were Don Carlo and Gentile di Anghiari, which had a slightly lower productive performance than Arbequina. Overall, the results are encouraging and suggest that some of these cultivars may be suitable candidates for high- and super-high-density olive orchards. This suitability is further supported by their favorable fruit characteristics, which appear to facilitate efficient mechanical harvesting. However, additional data is necessary to enable a more comprehensive assessment of these cultivars, particularly their capacity to maintain canopy dimensions compatible with straddle harvester operation, while maintaining a stable vegetative–reproductive balance over time. Full article

The predatory mite Amblyseius andersoni Chant (Acari: Phytoseiidae) is a key biological control agent against spider mites and other pests. For its broad application, efficient and affordable mass-rearing systems are essential. This study evaluated the effects of rearing substrate, food type, and rearing history on the development, survival, reproduction, and predation efficiency of the predator. Mites were reared on leaf discs or Plexiglas plates and fed one of five diets, including various plant pollens and the stored product mite Carpoglyphus lactis (L.) (Acari: Carpoglyphidae). Additionally, it was assessed whether rearing five generations on cattail pollen supplemented with the natural prey (Tetranychus urticae Koch (Acari: Tetranychidae) or frozen C. lactis influenced later predators’ performance. The substrate type did not affect development or survival contrary to the food source, with mites fed on cattail pollen or C. lactis developing faster and producing more eggs. Survival remained high across all diets. The intrinsic rate of increase was highest with cattail pollen and C. lactis. The five-generation rearing did not affect performance or feeding on natural prey such as T. urticae or Aculops lycopersici (Tryon) (Acari: Eriophyidae). These findings demonstrate that A. andersoni can be effectively mass-reared on alternative diets and substrates, supporting biocontrol strategies. Full article

Vanadium is a transition metal whose environmental presence has increased due to human activities such as fossil fuel combustion and industrial processes. A central mechanism of its toxicity involves mitochondrial dysfunction, as vanadium exposure disrupts energy metabolism, enhances reactive oxygen species (ROS) generation, and triggers oxidative stress, ultimately leading to genetic damage and alterations in cellular signaling. These mitochondrial alterations contribute to its potential carcinogenic, immunotoxic, and neurotoxic properties, affecting multiple systems, including the neurological, renal, immune, and reproductive systems. Since there are no specific treatments for vanadium intoxication, natural compounds—particularly plant-derived metabolites with antioxidant, mitochondrial-targeted, and chelating properties—have been investigated as potential therapeutic agents to counteract its toxicity. In this context, simple models such as the nematode Caenorhabditis elegans (C. elegans), the fruit fly (Drosophila melanogaster), and the zebrafish (Danio rerio) have emerged as valuable experimental systems for studying vanadium-induced mitochondrial dysfunction and evaluating protective strategies. These organisms offer key advantages, including a short life cycle, ease of handling, and conservation of essential biological pathways with mammals, making them effective tools in environmental toxicology. The aim of this review is to outline the mitochondrial-related toxic effects of vanadium across different biological models and to explore plant-based therapeutic approaches capable of mitigating its harmful health impacts. We also propose the use of simple models, such as D. melanogaster, D. rerio, and, most notably, C. elegans, as versatile and complementary experimental platforms to advance research in this field. Full article

Lead (Pb) is a non-essential, toxic heavy metal with no known biological function that has caused widespread environmental contamination throughout human history. Pb toxicity represents one of the most persistent environmental health challenges, with no safe exposure threshold identified. The metal demonstrates remarkable persistence in biological systems, with approximately 90% of it stored in bone tissue for decades, mimicking calcium due to its similar ionic properties. Contemporary contamination primarily stems from mining activities, battery manufacturing, electronic waste recycling, and deteriorating infrastructure. Pb enters organisms through multiple pathways and causes severe health impacts across all biological systems, with particularly devastating neurodevelopmental and bone effects in children and cardiovascular and reproductive consequences in adults. On a molecular level, Pb disrupts cellular processes through ion mimicry, replacing essential metals in enzymes and proteins and leading to mitochondrial dysfunction, oxidative stress, DNA damage, and epigenetic modifications. This review examines the sources of Pb pollution and its toxicological impacts on bacteria, fungi, plants, animals, and humans. It explores the molecular mechanisms underlying these effects, including neuroinflammation, genotoxicity, and cell death pathways. The paper considers current approaches for Pb removal from contaminated environments and therapeutic interventions for Pb poisoning. Full article

The traditional use of medicinal plants around the world has a long history, predominantly in low- and middle-income countries. Previous ethnobotanical research pertaining to urban environments demonstrated that the legacy of the use of medicinal plant species persists worldwide; however, information about the main city in the occidental part of Mexico is scarce regarding this traditional knowledge and its variation during the last few decades. A database was created from interviews with local people who had inhabited the oldest neighborhoods of Guadalajara for at least 30 years and by using different electronic databases. In addition, the correct taxonomic identification of species was supported via corroboration through local and other digital herbariums. Furthermore, a Principal Coordinate Analysis (PCoA) was performed on the database information to search for relationships among the medicinal plant species used. An inventory of 137 medicinal plants was created, where the plant species most commonly used in the five old neighborhoods of Guadalajara City were muicle (Justicia spicigera Schltdl.), pirul (Schinus molle L.), manzanilla (Matricaria chamomilla L.), valeriana (Valeriana sp.), calabaza (Cucurbita pepo L.), cola de caballo (Equisetum arvense L.), tepezcohuite (Mimosa tenuiflora Poir.), salvia (Salvia officinalis L.), canela (Cinnamomum verum J. Presl.), tila estrella (Tilia americana var. mexicana (Schltdl.) Hardin), cedrón (Aloysia citrodora Paláu), uva (Vitis vinifera L.), jengibre (Zingiber officinale Roscoe) and gobernadora (Larrea tridentata (DC.) Coville). Illnesses of the cardiovascular, digestive, urinary, respiratory, nervous, muscular and reproductive systems, as well as culture-bound syndromes, were mostly treated with these plant species. Moreover, J. spicigera, M. chamomilla and L. tridentata were used for eight medical purposes, followed by Z. officinale with five medicinal practices. In contrast, only two medicinal uses were recorded for C. pepo, M. tenuiflora and S. officinale. The PCoA explained 65.88% of the variation accumulated at the first three ordination axes and formed four groups of species, which were related to their geographical origin. Eight of the fourteen species that are commonly used as medicinal plants are from America, and the rest come from Europe and Asia. This study confirms the persistence of traditional knowledge related to medicinal plants, and the diseases empirically addressed among the inhabitants of Guadalajara City are common in other parts of the world and in different regions of Mexico. These findings are supported by electronic databases that comprise multiple studies related to the phytochemical compounds and medical validation regarding their biological activity, supporting the empirical use and efficacy of these medicinal plants. Full article