Search Results (577)

Weed management remains a critical challenge in the U.S. container ornamental production industry, where weeds not only compete with crops for limited resources but also harbor pests and pathogens, thereby diminishing plant quality and marketability. The paper explores the economic impact of weed infestations, herbicide resistance development, and the limited availability of selective herbicides for ornamental crops in the United States. This review synthesizes current chemical weed control tactics, focusing not only on both preemergence and postemergence herbicides commonly used in ornamental nurseries, but also organic alternatives and integrated weed management (IWM) approaches as complementary strategies by evaluating their effectiveness, crop safety, and usage. There is a critical need for research in the areas of alternative chemical options such as insecticides, miticides (e.g., Zerotol and Tetra Curb Max), and organic products for liverwort control in greenhouses. Although essential oils and plant-based extracts show some potential, their effectiveness and practical use remain largely unexplored. Full article

The commercial propagation of strawberries is increasingly constrained by the incidence of both established and emerging soilborne pathogens, particularly under soil cultivation systems. Micropropagation represents an effective strategy to ensure the production of virus-free, true-to-type mother plants suitable for high-efficiency propagation. In this study, micropropagated mother plants of four short-day cultivars (‘Francesca’, ‘Silvia’, ‘Lauretta’, and ‘Dina’) and one ever-bearing advanced selection (‘AN12,13,58’) were cultivated under a controlled soilless system. Quantitative parameters including number of runners per plant, runner length, and number of tips per runner and per plant were assessed to evaluate propagation performance. Micropropagated mother plants exhibited a significantly higher stoloniferous potential compared to in vivo-derived mother plants (frigo plants type A), with the latter producing approximately 50% fewer propagules. Rooted tips of ‘Dina’ were further assessed under different fertigation regimes. The NPK 20–20–20 nutrient solution enhanced photosynthetic activity and shoot and root biomass (length, diameter, and volume via WinRHIZO analysis). These results confirm the suitability of micropropagated mother plants grown in soilless conditions for efficient, high-quality clonal propagation and support the integration of such systems into certified nursery production schemes. Full article

Almond decline and dieback have become significant challenges in newly established orchards, with symptoms including internal necrosis, canker, and external gummosis. This work aims to explore the potential fungal and bacterial causative agents through metabarcoding and traditional culture plate isolation across six almond cultivars. Our results emphasize the multifactorial nature of almond decline and dieback, with possible co-infections by opportunistic fungi and bacteria playing a central role. Classical isolation identified 47 fungal species or genera, including Diaporthe amygdali, Diplodia corticola, Phytophthora sp., and several Fusarium species. Almond metabarcoding revealed a more diverse microbial community, highlighting the prevalence of soilborne pathogens such as Neocosmospora rubicola, Dactylonectria estremocensis, and Plectosphaerella niemeijerarum. Soil metabarcoding suggested that these pathogens likely originate from nursery substrates or soils shared with other crops, such as olives and vineyards, that serve as a source of inoculum. ‘Soleta’ generally presented lower richness when compared to the other tested cultivars, suggesting a higher degree of biotic stress and decreased plant resilience. This study highlights the value of integrating NGS approaches to comprehensively study complex diseases and the need for further research on pathogen interactions and cultivar susceptibility for the future development of new sustainable, targeted management strategies in almond orchards. Full article

Restricting the use of chemical pesticides in forestry requires the search for alternative solutions. These could be volatile organic compounds produced by three investigated species of bacteria (Bacillus amyloliquefaciens (ex Fukumoto) Priest, B. subtilis (Ehrenberg) Cohn and B. thuringiensis Berliner), which inhibit the growth of the pathogen F. oxysporum Schltdl. emend. Snyder & Hansen in forest nurseries. The highest inhibition of fungal growth (70%) was observed with B. amyloliquefaciens after 24 h of antagonism test, which had a higher content of carbonyl compounds (46.83 ± 8.41%) than B. subtilis (41.50 ± 6.45%) or B. thuringiensis (34.62 ± 4.77%). Only in the volatile emissions of B. amyloliquefaciens were 3-hydroxybutan-2-one, undecan-2-one, dodecan-5-one and tetradecan-5-one found. In contrast, the main components of the volatile emissions of F. oxysporum were chlorinated derivatives of benzaldehyde (e.g., 3,5-dichloro-4-methoxybenzaldehyde) and chlorinated derivatives of benzene (e.g., 1,4-dichloro-2,5-dimethoxybenzene), as well as carbonyl compounds (e.g., benzaldehyde) and alcohols (e.g., benzyl alcohol). Further compounds were found in the interactions between B. amyloliquefaciens and F. oxysporum (e.g., $\alpha$-cubebene, linalool, undecan-2-ol, decan-2-one and 2,6-dichloroanisole). Specific substances were found for B. amyloliquefaciens (limonene, nonan-2-ol, phenethyl alcohol, heptan-2-one and tridecan-2-one) and for F. oxysporum (propan-1-ol, propan-2-ol, heptan-2-one and tridecan-2-one). The amounts of volatile chemical compounds found in B. amyloliquefaciens or in the bacterium–fungus interaction can be used for further research to limit the pathogenic fungus. In the future, one should focus on the compounds that were found exclusively in interactions and whose content was higher than in isolated bacteria. In order to conquer an ecological niche, bacteria increase the production of secondary metabolites, including specific chemical compounds. The results presented are a prerequisite for creating an alternative solution or supplementing the currently used methods of plant protection against F. oxysporum. Understanding and applying the volatile organic compounds produced by bacteria can complement chemical plant protection against the pathogen, especially in greenhouses or tunnels where plants grow in conditions that favour fungal growth. Full article

Seed germination is a critical phase in the plant lifecycle of Camellia oleifera (oil tea), directly influencing seedling establishment and crop reproduction. In this study, we examined transcriptomic and physiological changes across five defined germination stages (G0–G4), from radicle dormancy to cotyledon emergence. Using RNA sequencing (RNA-seq), we assembled 169,652 unigenes and identified differentially expressed genes (DEGs) at each stage compared to G0, increasing from 1708 in G1 to 10,250 in G4. Functional enrichment analysis revealed upregulation of genes associated with cell wall organization, glucan metabolism, and Photosystem II assembly. Key genes involved in cell wall remodeling, including cellulose synthase (CESA), phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), caffeoyl-CoA O-methyltransferase (COMT), and peroxidase (POD) showed progressive activation during germination. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed dynamic regulation of phenylpropanoid and flavonoid biosynthesis, photosynthesis, carbohydrate metabolism, and hormone signaling pathways. Transcription factors such as indole-3-acetic acid (IAA), ABA-responsive element binding factor (ABF), and basic helix–loop–helix (bHLH) were upregulated, suggesting hormone-mediated regulation of dormancy release and seedling development. Physiologically, cytokinin (CTK) and IAA levels peaked in G4, antioxidant enzyme activities were highest in G2, and starch content increased toward later stages. These findings provide new insights into the molecular mechanisms underlying seed germination in C. oleifera and identify candidate genes relevant to rootstock breeding and nursery propagation. Full article

The nursery phase is vital for forest regeneration, yet studies on plant growth-promoting (PGP) bacteria to enhance sustainable nursery production in forest species are scarce. This study explores whether endophytic bacteria from disease-resistant Pinus pinea L. can improve germination and seedling quality in susceptible Pinus radiata D. Don. Root endophytes were isolated, screened for PGP traits, and identified via 16S rRNA gene sequencing. Bacterial formulations were applied to P. radiata seeds to determine their impact on germination and plant quality indicators (photosynthetic pigments and other metabolites). Paenibacillaceae (19%) and Bacillaceae (13%) were predominant among 68 isolates, with 94% producing indole-3-acetic acid, and Burkholderiaceae showing the broadest PGP trait diversity. Seedlings inoculated with formulation C3 (Caballeronia R.M3R3, Rhodococcus T.M4R4, and Mesorhizobium R.M1R2) displayed an improved germination rate (89% compared to 71% from the uninoculated control), while those inoculated with formulation P4 (Paenibacillus T.M5R4, Bacillus R.M2R7, Acinetobacter T.M2R22, and Paraburkholderia R.M1R3) showed an improved germination rate (81%), increased amount of starch (0.4-fold), and free amino acids (1.5-fold). This study presents a comprehensive approach, from endophyte isolation to in vivo tests, highlighting two bacterial formulations as candidates for further proof-of-concept nursery trials. Ultimately, these bioinoculants represent eco-friendly strategies to enhance forest seedling establishment and support sustainable forest management. Full article

Juglans neotropica Diels, an Andean native species classified as endangered by the IUCN, holds significant potential for reforestation and sustainable forest management programs. This study evaluated seed quality, phenotypic variability, and early establishment under nursery and field conditions in southern Ecuador. Three provenance sites—The Tundo, The Victoria, and The Argelia—were evaluated during the nursery phase, and two (The Tundo and The Victoria) in plantations, applying four pre-germination treatments: control, mechanical scarification, hot water, and water-sun exposure. Parameters assessed included seed weight, size, viability, germination, survival, and growth across three planting environments: secondary forest, riparian forest, and pasture. Significant differences in seed morphometry were observed among localities, while germination was influenced by treatment but not provenance. Seed viability remained high for up to six months, decreasing with a 2% loss of moisture. Survival reached 100% with urea application, and 96% of individuals exhibited straight stems after one year. No significant differences in growth were found between localities; however, basal diameter was highest in the pasture (13.2 mm/year⁻¹), and total height was greatest in the secondary forest (54.8 cm/year⁻¹). These findings provide key technical evidence to optimize the propagation and establishment of J. neotropica in ecological restoration and forest production contexts. Full article

Sustainable alternatives to peat in horticultural substrates are increasingly sought. This study assessed the use of coffee silverskin (CS), a byproduct of coffee roasting, as a substrate component for rooting and growing ornamental plants—Buddleja, Lythrum, and Veronica. Plants were cultivated in peat-based substrates with 0%, 25%, 50%, and 75% CS addition. In order to determine the effect of substrate modification with CS, the following parameters were analyzed: rooting efficiency, plant growth, pigment content, physiological indices (SPAD, Fv/Fm, NFI), and substrate properties. A 25% CS addition supported high rooting success (94.4% on average) without negatively affecting root or shoot traits, and even improved flowering earliness. At 50% CS, Buddleja showed moderate tolerance, while Lythrum and Veronica performed poorly. The substrate with 75% CS addition significantly reduced rooting and growth across all species. Elevated pH and electrical conductivity in high-CS substrates likely contributed to plant stress. Physiological indicators confirmed minimal stress at 25% CS, but increased stress response at 75%. Overall, CS can replace up to 25% of peat in substrates without compromising plant performance, offering a sustainable alternative in nursery production. However, higher CS levels require structural or chemical adjustments to reduce compaction and stress effects. Further research is needed to improve CS-based substrate formulations for broader horticultural use. Full article

Phenolic compounds, which are a large group of plant pigments, are recognized as important antioxidants. The potato (Solanum tuberosum L.), particularly the pigmented varieties, could be a source of natural anthocyanins for producing dietary foods. In this study, we analyzed forty potato specimens from our germplasm collection and breeding nurseries using high-performance liquid chromatography (HPLC) and second-order mass spectrometry to identify anthocyanins. We found seven main anthocyanins in potato tubers: delphinidin-3-glucoside, delphinidin-3-rhamnosyl-5-glucoside, petunidin-3-glucoside, malvidin-3-glucoside, cyanidin-3-glucoside, cyanidin-3-rhamnosyl-5-glucoside, and pelargonidin-3-glucoside. Two anthocyanins were found in potato inflorescences: peonidin-3-coumaroyl glucoside and cyanidin-3-coumaroyl glucoside. On average, varieties from the group with red-purple inflorescences contained 187.6 mg/kg of anthocyanins. Genotypes with white corollas had an anthocyanin content below 0.5 mg/kg or between 1.3 and 3.6 mg/kg. Two potato varieties, Vasilek (605.2 mg/kg) and Fioletovyi (501.1 mg/kg), with blue-purple corollas, had the highest total anthocyanin content. Studying the anthocyanin profile of leaves allowed us to identify eleven anthocyanins. The highest anthocyanin content (331.3 mg/kg) was found in varieties with purple or blue-purple tubers, while the lowest content (an average of 15.1 mg/kg) was found in varieties with yellow or cream tubers. Genotypes with purple and blue-purple tuber skin had an average anthocyanin content of 190.7 mg/kg. The group with yellow and cream tubers had an insignificant anthocyanin content (1.2 mg/kg). Varieties from the group with pink tubers had an average anthocyanin content of 43.2 mg/kg. Thus, this study identified diagnostic traits that could be used to assess the morphological characteristics of potato genotypes. Full article

The introduction of non-native species like Xylella fastidiosa to new environments can lead to potentially catastrophic ecological and economic repercussions. This work comprehended the prospection phase (insect sampling and submission of samples to the laboratory) from X. fastidiosa demarcated zones of Área Metropolitana do Porto; Sabrosa; Alijó; Baião; Mirandela; Mirandela II; and Bougado and the research phase (collecting and organizing data and statistical treatment). The results of this study showed the presence of the bacterium in some tested spittlebugs species captured in DZ of Área Metropolitana do Porto, which highlights the role of the vector in mediating the disease’s propagation. Most insects were found in public gardens and in nurseries/gardens where there is a diverse array of food sources, shelter, mating locations, and suitable substrates for egg laying that serve as ideal conditions for the population of Philaenus spumarius. We observed that most insects were found in the first trimester (36.5%), followed by the third trimester (23.2%). Finally, it was shown that, in our study, the most frequent host plants where insects were found included Lavandula dentata, Ulex minor, Ulex europaeus, Quercus suber, Plantago lanceolata. Our findings imply a robust connection between plant communities, ecological conditions, and insect populations with the occurrence of Xylella fastidiosa, particularly within the examined climatic context. Full article

This study addressed a significant and relevant issue, specifically the production of high-quality fruit planting material linked to an economically viable nursery operation. The process considered both the pedo-climatic conditions of the region where the fruit planting material was cultivated and the technological elements utilized. The objective of this research was to gather information regarding the necessity and effectiveness of implementing localized irrigation for plum trees in the nursery in the context of various fertilization treatments. It also aimed to investigate the variations in leaf area among Cacanska Lepotica and Stanley plum cultivars subjected to various irrigation (non-irrigated control, 10 mm, 20 mm, and 30 mm) and fertilization (unfertilized control, N₈P₈K₈, N₁₆P₁₆K₁₆, and N₂₄P₂₄K₂₄) methods. The study was conducted within a private nursery situated in the northwest region of Romania using a 4 × 2 × 4 split-split-plot design with five replications. This research took place in the summer of 2024, in the second field of the nursery during the growth stage of grafted trees. The implementation of various NPK fertilization methods (8%, 16%, and 24%) led to enhancements in leaf surface developments (increased by 6.53–16.14% compared to the control). The application of fertilization ranging from 8 to 16% and subsequently from 16 to 24% was effectively absorbed by the plum trees, resulting in a substantial growth of 180–226 cm². Irrigation with 30 mm generated significant increases in the leaf area of 4.42–14.27% compared to the control. To obtain optimal yields of grafted trees, it is advisable to utilize a combination of irrigation and NPK fertilization. To promote the appropriate growth and development of the trees, it is essential to monitor the soil moisture levels and to implement irrigation during times of water shortage when the trees exhibit heightened water usage. The research findings indicated that both cultivars experienced similar advantages from 24% NPK fertilization and 30 mm of irrigation; therefore, the implementation of the aforementioned technological elements is strongly recommended. Full article

A quantitative literature review of restoration techniques and supporting management strategies used throughout the Caribbean and Western Atlantic from 1998 through 2024 was compiled using references from the Web of Science to highlight those with potential for reef replenishment. From 93 sources listed, 74 publications were relevant and categorized into subtopics based on the most prevalent restoration techniques. Roughly half the studies focused on three general topics: the benefits of restoring Acropora species, studies utilizing micro-fragmentation and fragment nurseries, and outplanting techniques. Other subtopics, each with at least three references, included optimizing substrates and artificial reefs, enhancing larval recruitment, emphasizing the role of herbivory, improving management practices, and addressing the impacts of tourism and community engagement. The information from the references was compiled to determine the overlap among categories and the ways in which techniques and management strategies might be applied simultaneously to enhance restoration outcomes. Additionally, sources were analyzed according to time and location of publication to better visualize the emergence of this area of research and restoration efforts. An increase in publications was observed from 2014 to 2024, associated with the rise in major events impacting coral reefs. The major locations for published research were the Florida reef tract and Puerto Rico, though restoration studies were also reported from the Bahamas and sites around the Caribbean. Criteria to assess the success of techniques included coral survival, recruitment, coral coverage, habitat structure and complexity, and biomass of marine life, including fish and invertebrates that inhabited a restored reef. Most restoration efforts utilized either fragmentation or assisted sexual breeding, followed by cultivation in nurseries or labs. Outplanting success depended on fragment size, attachment style, and site selection, with less-intrusive techniques and intermediate planting densities promoting survival. Tools like GAO maps can guide site selection based on herbivore presence and algal coverage. Monitoring is critical to ensuring coral survival, especially after the first year of outplanting, while community involvement can foster public engagement in reef conservation. Full article

Olive knot disease, caused by Pseudomonas savastanoi pv. savastanoi, severely impacts olive tree yield and oil quality. Early and accurate detection of the bacterium’s presence, particularly in asymptomatic plants, is crucial for effective disease management. This study aimed to develop an improved protocol for processing plant samples and adapting quantitative PCR to droplet digital PCR (ddPCR). For this purpose, four plant preparations—EW (external washing), PELLET (bacterial concentration), and enrichment in liquid media for 24 or 48 h (24hE, 48hE)—were tested using spiked samples. The ddPCR was set up and compared with qPCR to evaluate analytical sensitivity and specificity. Additionally, field samples from symptomatic and asymptomatic olive orchards were tested to evaluate the performance of the selected methods in naturally infected plants. ddPCR showed higher sensitivity than qPCR, particularly with the PELLET and 24hE preparations. The PELLET from the spiked sample preparation achieved a limit of detection of 10 CFU/mL for both molecular tests. The ddPCR, combined with the PELLET preparation, offers a highly sensitive and reliable tool for detecting P. savastanoi pv. savastanoi in asymptomatic olive material. This protocol shows great potential for improving early bacterial detection and disease prevention, thus aiding control strategies in nurseries and olive orchards, and supporting the production of certified plant propagation material. Full article

Diseases and weeds occupy a leading place among the factors limiting the yield of agricultural crops, including rice. These factors can be overcome through the use of chemical protective agents, as well as through the creation and introduction into agricultural production of rice varieties resistant to these stressors. The use of DNA marking technologies for target genes of economically valuable traits in the creation of promising varieties allows not only for the identification of genes but also the monitoring of their transmission during crosses and the selection of breeding-valuable genotypes with genes of interest. In addition, this ensures a reduction in the volume of breeding nurseries, as well as time and material costs during variety modeling, and rapid rotation of new high-yield varieties with specified characteristics. We have selected effective marker systems based on the use of DNA marking technologies for target genes for resistance to blast (Pi) and submergence tolerance (Sub1A). These systems allow for precise targeted selection of hybrid plants with these genes in the breeding process. In addition, we have automated the detection of transferred Pi-ta and Pi-b genes, which greatly relieves the DNA analysis during mass screening of breeding material. The final result of this work is the created rice varieties Al’yans, Lenaris and Kapitan with the Pi-ta blast resistance gene and the Pirouette rice variety with the Pi-1, Pi-2, and Pi-33 genes. These varieties exceed the standards by 0.64–2.2 t/ha, and their involvement in production makes it possible to obtain additional products by increasing yields in the amount of about RUB 80 thousand/ha. Full article

The use of preemergence herbicides is the primary method of controlling weeds in container-grown ornamental plants, but it may cause injury to common popular ornamentals. The objective of this research was to evaluate the use of overhead irrigation to reduce phytotoxicity in ornamental plants. Dimethenamid-P and flumioxazin were applied at standard label rates to container-grown coneflower (Echinacea purpurea), lady fern (Anthyrium filix-femina), and blue plumbago (Plumbago auriculata). Plants were subjected to one of four irrigation regimes at the time of herbicide treatment, including receiving 1.3 cm of overhead irrigation before treatment, immediately after treatment, both immediately before and after treatment, and no irrigation until the next irrigation cycle resumed at 4 h after treatment. For all three species, irrigation timing had minimal effect on visual injury ratings following treatment with dimethenamid-P, as injury was minimal overall. Severe injury was observed following treatment with flumioxazin, but significant recovery was noted in both lady ferns and echinacea when irrigation was applied immediately after treatment. The results indicate that irrigating plants immediately after treatment could improve crop tolerance to preemergence herbicide applications and should be further investigated as an injury management strategy for container-grown ornamental plants. Full article