Search Results (662)

Chronic kidney disease (CKD)-associated anemia is a global health concern and is linked to vascular and ocular complications. Hypoxia-inducible factor (HIF) stabilizers, or HIF prolyl hydroxylase inhibitors (PHIs), are promising candidates for the treatment of CKD-associated anemia. Since hypoxia and angiogenesis are involved in eye diseases, this study examined the effects of HIF-PHIs on metabolism and gene expression in retinal pigment epithelium (RPE) cells. Results revealed that PHIs differentially induced angiogenic (VEGFA, ANG) and glycolytic (PDK1, GLUT1) gene expression, with Roxadustat causing the strongest transcriptional changes. However, Roxadustat-induced angiogenic signals did not promote endothelial tube formation. Moreover, it did not induce oxidative stress, inflammation, or significant antioxidant gene responses in ARPE-19 cells. Roxadustat also reduced the inflammatory cytokine response to tumor necrosis factor-α, including IL-6, IL-8, and MCP-1, and did not exacerbate VEGF expression under high-glucose conditions. Overall, Roxadustat triggered complex gene expression changes without promoting inflammation or oxidative stress in RPE cells. Despite these findings, ophthalmologic monitoring is advised during PHI treatment in CKD patients receiving HIF-PHIs. Full article

Biofloc technology (BFT), traditionally centered on feed supplementation and water purification in aquaculture, harbors untapped multifunctional potential as a sustainable resource management platform. This review systematically explores beyond conventional applications. BFT leverages microbial consortia to drive resource recovery, yielding bioactive compounds with antibacterial/antioxidant properties, microbial proteins for efficient feed production, and algae biomass for nutrient recycling and bioenergy. In environmental remediation, its porous microbial aggregates remove microplastics and heavy metals through integrated physical, chemical, and biological mechanisms, addressing critical aquatic pollution challenges. Agri-aquatic integration systems create symbiotic loops where nutrient-rich aquaculture effluents fertilize plant cultures, while plants act as natural filters to stabilize water quality, reducing freshwater dependence and enhancing resource efficiency. Emerging applications, including pigment extraction for ornamental fish and the anaerobic fermentation of biofloc waste into organic amendments, further demonstrate its alignment with circular economy principles. While technical advancements highlight its capacity to balance productivity and ecological stewardship, challenges in large-scale optimization, long-term system stability, and economic viability necessitate interdisciplinary research. By shifting focus to its underexplored functionalities, this review positions BFT as a transformative technology capable of addressing interconnected global challenges in food security, pollution mitigation, and sustainable resource use, offering a scalable framework for the future of aquaculture and beyond. Full article

The oxidative degradation of anthocyanins in red wine was investigated under controlled conditions using hydroxyl radicals generated in the presence of Cu (II) as a catalyst. A full factorial experimental design with 23 replicates was used to evaluate the effects of hydrogen peroxide concentration, catalyst dosage, and reaction temperature on anthocyanin degradation over a fixed time. Statistical analysis (ANOVA and multiple regression) showed that all three variables and the main interactions significantly affected anthocyanin loss, with temperature identified as the most influential factor. The combined effects were described by a first-order polynomial model. The activation energies for degradation ranged from 56.62 kJ/mol (cyanidin-3-O-glucoside) to 40.58 kJ/mol (peonidin-3-O-glucoside acetate). Increasing the temperature from 30 °C to 40 °C accelerated the degradation kinetics, almost doubled the rate constants and shortened the half-life of the pigments. At 40 °C, the half-lives ranged from 62.3 min to 154.0 min, depending on the anthocyanin structure. These results contribute to a deeper understanding of the stability of anthocyanins in red wine under oxidative stress and provide insights into the chemical behavior of derived pigments. The results are of practical importance for both oenology and viticulture and support efforts to improve the color stability of wine and extend the shelf life of grape-based products. Full article

Annually, more than 10,000 synthetic dyes are produced worldwide, generating around 280,000 tons of waste, posing risks to human and aquatic life, and potentially creating even more toxic products than the dyes themselves. This study aims to immobilize organic dyes, forming hybrid pigments using ZnO as support obtained through starch combustion. ZnO was obtained by starch (sago) combustion and characterized by XRD, SEM and the BET method. It was then used for the adsorption of orange and green textile dyes, evaluating the adsorbent dosage, initial dye concentration, contact time, and selectivity with copper ions. The removal studies indicated up to 100% removal of both dyes at low concentrations. The co-adsorption system showed excellent performance, with removal percentages exceeding 90% for both textile dyes and Cu (II) ions. Hybrid pigments were assessed for solvent resistance and durability under extended white light exposure. ZnO immobilized the dyes, showing resistance to organic solvents and good stability under prolonged white light exposure. Full article

The restoration of gold leaf on sandstone sculptures requires structural stability, aesthetic considerations, and compliance with the principles of cultural heritage preservation. A primary issue is achieving visual and material compatibility between newly restored and original areas. Based on the “Diagnosis–Analysis–Selection–Restoration” methodology, the research team developed a targeted restoration approach for gilded stone sculptures, using the Shakyamuni sculpture at Erfo Temple in Chongqing as a case study. Assessment of the current situation revealed that over 70% of the sculpture’s surface exhibited gold leaf delamination. The composition and structure of the gold-sizing lacquer, lacquer plaster filler, ground layers, and pigments were investigated using SEM-EDS, XRD, Raman spectroscopy, and THM-Py-GC/MS techniques. The results confirmed that the sculpture featured a typical multilayer gilding structure with clear evidence of historical restorations. Considering both material performance and interfacial compatibility, an NHL2/SiO₂/SF016 composite emulsion and traditional lacquer plaster were selected as the optimal materials for reattachment and infill, respectively. A scientific restoration protocol was developed, encompassing gentle cleaning, targeted reattachment and reinforcement, and region-specific repair methods. Principal Component Analysis (PCA) was used to evaluate the influence of temperature and humidity on the curing behavior of lacquer layers. Additionally, a non-invasive gold leaf color-matching technique was developed by controlling the surface roughness of the gold-sizing lacquer, effectively avoiding the damage caused by traditional color-matching methods. Full article

The clean-label movement has markedly increased consumer demand for meat products free from synthetic additives, such as sodium nitrite, ascorbate, and phosphate. This review summarizes strategies to replace these additives with natural alternatives while preserving the functional and quality properties of traditionally cured meats. Nitrite replacement commonly employs nitrate-rich vegetables, alongside nitrate-reducing starter cultures or pre-converted nitrite powders for adequate nitric oxide production and meat pigment stabilization. Ascorbate substitutes include vitamin C-rich materials and polyphenol-based antioxidants from green tea and rosemary, supporting nitrite reduction and contributing to meat pigment and oxidative stability. To compensate for phosphate functions, natural substitutes such as hydrocolloids, dietary fibers, protein isolates, and calcium powders from eggshells or oyster shells have shown partial success in restoring water-holding capacity, pH buffering, and textural integrity. In addition, non-thermal processing technologies, such as high-pressure processing, ultrasound, and cold plasma are explored as complementary strategies to enhance the efficacy of natural ingredients and support industrial scalability. However, challenges persist regarding ingredient variability, dose-dependent effects, and consistency in functional performance. Future research should focus on synergistic ingredient combinations, formulation standardization, and scalable application in industrial production to ensure the production of high-quality clean-label meat products. Full article

Central nervous system diseases are highly complex in terms of etiology and pathogenesis, making their treatment and interventions for them a major focus and challenge in neuroscience research. Anthocyanins, natural water-soluble pigments widely present in plants, belong to the class of flavonoid compounds. As natural antioxidants, anthocyanins have attracted extensive attention due to their significant functions in scavenging free radicals, antioxidation, anti-inflammation, and anti-apoptosis. The application of anthocyanins in the field of central nervous system injury, particularly in neurodegenerative diseases, neurotoxicity induced by chemical drugs, stress-related nerve damage, and cerebrovascular diseases, has achieved remarkable research outcomes. However, anthocyanins often exhibit low chemical stability, a short half-life, and relatively low bioavailability, which limit their clinical application. Recent studies have found that the stability and bioavailability of anthocyanins can be significantly improved through nanoencapsulation, acylation, and copigmentation, as well as the preparation of nanogels, nanoemulsions, and liposomes. These advancements offer the potential for the development of anthocyanins as a new type of neuroprotective agent. Future research will focus on the innovative design of nano-delivery systems and structural modification based on artificial intelligence. Such research is expected to break through the bottleneck of anthocyanin application and enable it to become a core component of next-generation intelligent neuroprotective agents. Full article

This study investigates the thermally induced phase transformation of Ni-exchanged LTA zeolite as a dual-purpose method for nickel immobilization and the synthesis of stable ceramic pigments. The process describes a cost-effective and sustainable alternative to conventional pigment production, aligning with circular economy principles. Upon thermal treatment at temperatures ranging between 900 °C and 1300 °C, Ni-exchanged LTA zeolite undergoes a transformation to NiAl₂O₄ spinel, confirmed by XRPD, FTIR, and thermal analysis. Initially, NiO is formed, but as the temperature increases, it dissolves and transforms into NiAl₂O₄. Colorimetric studies revealed intensified blue pigmentation with increasing temperature, correlating with crystallite growth and structural evolution. SEM analysis showed morphological changes from cubic particles to sintered agglomerates, enhancing pigment stability and hardness. The Ni-LTA sample calcined at 1300 °C showed the highest hue angle, which was consistent with the formation of over 99 wt.% of the nickel aluminate crystalline phase at this temperature. The results demonstrate that Ni-LTA zeolite can be effectively transformed into durable greenish-blue pigments suitable for application in porcelain. This transformation is especially evident at 1300 °C, where a spinel phase (NiAlSi₂O₄) forms, with colorimetric values: L = 58.94, a* = –16.08, and b* = –15.90. Full article

Paracoccidioides brasiliensis is a thermally dimorphic fungal pathogen and the main etiological agent of paracoccidioidomycosis (PCM), a neglected systemic mycosis endemic in Latin America. The virulence of P. brasiliensis is closely associated with its capacity to survive under hostile host conditions, including acidic environments. In this study, we demonstrate that acidic pH induces melanization in P. brasiliensis, modulates its cell wall composition, and alters the interaction with macrophages. Cultivation at acidic pH resulted in reduced fungal growth without compromising viability and triggered increased production of melanin-like pigments, as confirmed by enhanced laccase activity and upregulation of genes in the DHN-melanin biosynthetic pathway. Additionally, growth under acidic pH induced significant remodeling of the fungal cell wall, leading to increased chitin on the cell wall surface and reduced mannan content, while β-glucan levels remained unchanged. These modifications correlated with decreased viability to Congo Red, suggesting altered cell wall stability. Importantly, P. brasiliensis grown under acidic conditions exhibited reduced phagocytosis by RAW 264.7 macrophages, along with changes in nitric oxide and cytokine production, indicating potential mechanisms of immune evasion. Collectively, our findings suggest that environmental acidification promotes fungal adaptations that enhance survival and modulate host–pathogen interactions, contributing to P. brasiliensis virulence. Understanding how acidic pH regulates these processes provides new insights into the pathobiology of PCM and may contribute to understanding the mechanisms of fungal immune evasion. Full article

Preserving heritage sites is a complex challenge that requires multidisciplinary approaches, combining scientific accuracy with cultural and historical sensitivity. In alignment with UNESCO’s conservation guidelines, this study investigated the airflow dynamics and wind-induced structural effects within ancient architecture using advanced computational fluid dynamics (CFD). The study site was the Na Phra Meru Historical Temple in Ayutthaya, Thailand, where the shear stress transport $k-\omega$ turbulence model was applied to analyze distinctive airflow patterns. A high-precision 3D computational domain was developed using Faro focus laser scanning technology, with the CFD results being validated based on onsite experimental data. The findings provided critical insights into the temple’s ventilation behavior, revealing strong correlations between turbulence characteristics, wind speed, temperature, and relative humidity. Notably, the small slit windows generated complex flow mixing, producing a large internal recirculation zone spanning approximately 70% of the central interior space. In addition to airflow distribution, the study evaluated the aerodynamic forces and rotational moments acting on the structure based on five prevailing wind directions. Based on these results, winds from the east and northeast generated the highest aerodynamic loads and rotational stresses, particularly in the lateral and vertical directions. Overall, the findings highlighted the critical role of airflow and wind-induced forces in the deterioration and long-term stability of heritage buildings. The study demonstrated the value of integrating CFD, environmental data, and structural analysis to bridge the gap between conservation science and engineering practice. Future work will explore further the interactions between wall moisture and the multi-layered pigments in mural paintings to inform preservation practices. Full article

Color, aroma, and overall sensory quality in red wines are largely influenced by oxygen availability during fermentation. This study evaluated the effects of micro-oxygenation under hyperbaric conditions on the physicochemical, chromatic, volatile, and sensory properties of Vitis vinifera L. cv. Monastrell grape must. Grape clusters were manually harvested and fermented under controlled conditions, applying micro-oxygenation treatments at two fermentation stages (day 3 and day 13) within a hyperbaric chamber. Physicochemical analyses, CIELab color measurements, visible reflectance spectra, GC-FID volatile profiling, and descriptive sensory analysis were performed. Micro-oxygenated samples (M1_MOX and M2_MOX) showed significant increases in lightness (L*), redness (a*), chroma (C*), and reflectance in the 520–620 nm range, indicating enhanced extraction and stabilization of phenolic pigments. Volatile analysis revealed that these samples also contained higher concentrations of key esters and terpenes associated with fruity and floral notes. Sensory evaluation confirmed these findings, with MOX-treated wines displaying greater aromatic intensity, flavor persistence, and varietal character. Control samples (M1_CON and M2_CON) exhibited lower color saturation and volatile compound content, along with diminished sensory quality. These results suggest that hyperbaric micro-oxygenation is an effective strategy for improving color intensity and aromatic complexity during red wine fermentation under controlled, non-thermal conditions. Full article

While pipecolic acid (Pip) mediates morpho-physiological and molecular responses during biotic stress, its roles under drought remain an inexpressible mystery. The investigation aimed to elucidate the roles of a 30μM Pip pretreatment in alleviating drought injury on barley (Hordeum vulgare L. cv, Bülbül89) seedlings. Pip pretreatment under normal or drought conditions lowered the osmotic potential (Ψs) and water saturation deficit (WSD), while optimizing the relative water content (RWC), triggered osmotically energetic molecules (OEM) and salicylic acid (SA) accumulation, improving osmotic adjustment (OA), and boosting water retention and uptake capacity (WTC, and WUC), alongwith a considerable improvement in seedling growth over non-treated plants under such conditions. Additionally, Pip pretreatment improved chlorophyll (Chl), the chlorophyll stability index (CSI), pheophytin_a, chlorophyllide_a (chlide_a), chlorophyllide_b (chlide_b), chl_a/chlide_a, chl_b/chlide_b, protoporphyrin, Mg-protoporphyrin, protochlorophyllide, and photosynthetic performance over non-treated plants under such conditions. Pip pretreatment preserves redox homeostasis in drought-stressed plants by accumulating antioxidant solutes alongside the activation of superoxide dismutase and glutathione reductase over non-treated plants. Drought distinctly reduced Ψs (more negative), RWC, photosynthetic pigment, CSI, chlorophyll assimilation intermediate, and photosynthetic performance, with an increment in chlorophyll degradation intermediate and nonenzymatic antioxidant solutes. Drought maintains OA capacity via a hyper-accumulation of OEM and SA, which results in higher WSD, WTC, and WUC. Drought triggered an oxidative burst, which was associated with a decline in the membrane stability index. These findings highlight Pip’s capability for lessening drought stress-induced restriction in barley seedlings via bolstering oxidative homeostasis, OA capacity, and stabilizing chlorophyll biosynthesis. Future research must elucidate the precise molecular mechanisms underlying Pip’s action in alleviating drought injury. Full article

Global fruit production is excessive, and fruit wine is a significant outcome of fruit processing. The pigment in fruit wine gives it a vibrant color and affects its quality, taste, and marketing. The pigments in fruit wines are commonly divided into three categories: anthocyanins, carotenoids, and chlorophylls. They are naturally synthesized pigments in plants that undergo complex biochemical changes that eventually tend to be stable in mature fruit wine, showing the color properties desired by consumers. Under normal circumstances, pigment molecules are unstable and have isomers, which makes it difficult to accurately identify and control them. In addition, biochemical changes produce a series of chemical derivatives that affect bioavailability and biological functions. This review summarizes the chemical basis, formation process, influencing factors, identification techniques, bioavailability, and bioactivity of fruit wine pigments, providing an important reference for the utilization of fruit resources and the development of high-quality fruit wine products. Full article

Chlorella vulgaris, a unicellular microalga with broad industrial applications, is a valuable source of bioactive compounds, including proteins, pigments, and lipids. However, optimizing its growth and metabolite production remains a challenge. This study investigates the potential of angular 6/6/5/6-annelated pyrrolidine-2,3-diones—structurally complex small molecules resembling alkaloids and 13(14 → 8)abeo-steroids—as novel growth stimulants for C. vulgaris. A series of these compounds (20 structurally diverse derivatives, including 7 previously unreported ones) were synthesized and screened for their ability to enhance microalgal growth. Primary screening identified one compound as a promising candidate, significantly increasing algae cell concentration in microplate cultures. Subsequent validation in flask-scale experiments revealed that this candidate induced a 19% increase in protein content at 1 μmol/L, suggesting potential for protein enrichment in algal biomass. Stability studies of the candidate compound revealed its significant hydrolytic degradation in aqueous media. These findings highlight the potential of angular 6/6/5/6-annelated pyrrolidine-2,3-diones as modulators of microalgal metabolism, offering a new avenue for enhancing C. vulgaris biomass quality, particularly for protein-rich applications in the food and feed industries. Full article

Marine biofouling, the process of marine microorganisms, algae, and invertebrates attaching to and forming biofilms on ship hulls, underwater infrastructure, and marine equipment in ocean environments, severely impacts shipping and underwater operations by increasing fuel consumption, maintenance costs, and corrosion risks, and by threatening marine ecosystem stability via invasive species transport. This study reports the development of a hydrogel-metal-organic framework (MOF)-quorum sensing inhibitor (QSI) antifouling coating on 304 stainless steel (SS) substrates. Inspired by mussel adhesion, a hydrophilic bionic hydrogel was first constructed via metal ion coordination. The traditional metal ion source was replaced with a zeolitic imidazolate framework-8 (ZIF-8) loaded with 2-(5H)-furanone (HF, a QSI) without altering coating formation. Physicochemical characterization using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), the Brunauer–Emmett–Teller (BET) method, and the diffraction of x-rays (XRD) confirmed successful HF loading into ZIF-8 with intact crystal structures. Antifouling tests showed HF@ZIF-8 enhanced antibacterial inhibition against Staphylococcus aureus (97.28%) and Escherichia coli (>97%) and suppressed Chromobacterium violaceum CV026 pigment synthesis at 0.25 mg/mL (sub-growth concentration). The reconstructed PG/PVP/PEI/HF@ZIF-8 coating achieved 72.47% corrosion inhibition via synergistic anodic protection and physical shielding. This work provides a novel green approach for surface antifouling and drag reduction, highlighting MOF-loaded QSIs as promising additives to enhance the antifouling performance of hydrogel coatings, anti-corrosion performance, and QSI performance for sustainable marine engineering applications. Full article