Search Results (189)

Campylobacter (C.) jejuni and C. coli are common zoonotic bacteria in pigs, which typically act as asymptomatic carriers. However, the effects of Campylobacter colonisation on the porcine intestinal microbiota and metabolome remain poorly understood. This study investigated microbiome and metabolome alterations associated with co-colonisation by C. jejuni and C. coli in the different intestinal segments of pigs. Thirty-two weaned piglets were assigned to a control group and a group inoculated with C. coli ST5777/CT828 and C. jejuni ST122/CT206. Four weeks post inoculation, jejunal and caecal contents were analysed for Campylobacter counts, metabolite profiles and microbial composition. All animals remained clinically healthy. Both Campylobacter species colonised the jejunum and caecum, with higher C. coli counts in the caecum. Campylobacter-colonised pigs showed significantly altered metabolite profiles, including reduced cysteine and urea and increased glycine in the jejunum, as well as elevated 3-hydroxybutyrate levels in the caecum. In contrast, short-chain fatty acid concentrations in the caecum were unaffected by infection. Microbiota analysis revealed a significant reduction in caecal alpha diversity, whereas jejunal diversity remained unchanged. Infected pigs exhibited increased relative abundances of Lactobacillaceae and Bifidobacteriaceae and a decreased abundance of Pseudomonadota, including Enterobacteriaceae. In conclusion, Campylobacter co-colonisation induces distinct microbiome and metabolome alterations in pigs despite the absence of clinical disease. These findings highlight complex host-microbiota–pathogen interactions that may be relevant for future Campylobacter control strategies in pig production. Full article

Background: Islatravir (ISL) is a first-in-class nucleoside reverse transcriptase translocation inhibitor with high potency and long half-life in peripheral blood mononuclear cells (PBMCs). However, treatment and prevention of HIV with oral ISL in humans has been associated with decreases in total lymphocytes, CD4 T-cells, and B-cells in a dose-dependent manner. We investigated in macaques the effects of oral ISL on lymphocytes, monocytes, granulocytes, and gene expression in PBMCs. Methods: Female pig-tailed macaques (n = 5) received an HIV pre-exposure prophylaxis dose of oral ISL adjusted allometrically once a week for 12 weeks. Complete blood counts and B- and T-cells were monitored prior to, during, and after ISL treatment, and changes in counts were evaluated by using a repeated measures model. Changes in gene expression were investigated in PBMCs during treatment and following treatment discontinuation. Results: ISL treatment was associated with declines in lymphocytes (11.9%, p = 0.0015) and monocytes (22.4%, p = 0.0003), but not granulocytes (0.3%, p = 0.9781). Total lymphocytes and monocytes returned to pre-treatment levels 6 weeks after treatment cessation (p = 0.8244 and p = 0.4620, respectively). Lymphocyte subpopulation analyses showed a significant decline in CD8 (−18.4%, p = 0.0364) and CD20 (−35.3%; p = 0.0002) cells but not CD4 cells (−7.4%; p = 0.3470). Gene set enrichment analysis showed negative enrichment (p_adj < 0.05) of gene pathways associated with immune regulation, cell proliferation, and inflammation. Conclusions: ISL treatment resulted in significant reductions in lymphocytes reproducing clinical toxicity. This effect was reversed after treatment cessation as observed in humans. Our results highlight the value of the macaque model to study immune alterations at the preclinical stage. Full article

The study aimed to determine the effect of newly developed multi-strain synbiotics on the hygienic quality of feed for pigs and pork edible raw materials originating from animals supplemented with synbiotics. The trial was conducted on 54 feed samples and 54 pigs. Animals were divided into six groups corresponding to three synbiotic preparations (A, B, C), two positive controls with probiotics (D, E), and a negative control (K) with no feed additive. The supplementation of the sow’s basal diet with the feed additive started 10 days before farrowing and continued for 38 days, covering the lactation period. The diet of piglets from groups A-E was supplemented with the feed additive starting from two weeks of age until slaughter at 24 weeks of age. Feed and animal raw materials were tested for the presence of Salmonella spp., Campylobacter spp., L. monocytogenes, Clostridium spp., C. perfringens, C. botulinum, and the count of AMB, TPC, fungi, ASFB, C. perfringens, Enterobacteriaceae family, E. coli, presumptive B. cereus, CoPS, HS, LAB, yeast probiotic strains, and Enterococcus spp. Statistically significant differences were found between individual groups in the count of C. perfringens, AMB, TPC, Enterococcus spp., and LAB in all feeds tested. Moreover, synbiotics A, B, and C lowered the count of AMB, TPC, and LAB, and synbiotics A and C decreased Enterobacteriaceae family contamination in both total raw materials as well as raw materials of fatteners. The higher effectiveness of synbiotics was associated with a higher number of probiotic strains in one preparation. Our study found that dietary supplementation of synbiotics alters the occurrence of C. perfringens in feed and enhances the hygienic quality of edible pork raw materials. Full article

This study evaluated the effects of supplementing a multi-strain probiotic (MSP) in low–crude protein (LP) diets on growth performance, apparent nutrient digestibility, selected fecal microbial populations, and nitrogen (N) utilization in weaned piglets. A 7-week feeding trial was conducted using 105 weaned crossbred piglets (Duroc × [Landrace × Yorkshire]) with an initial body weight of 6.55 ± 1.09 kg. Based on initial body weight and sex, piglets were randomly assigned to three dietary treatments using a randomized complete block design, with seven replicates per treatment and five pigs per pen (two gilts and three barrows). The dietary treatments included CON: normal crude protein (CP) diet; TRT1: LP diet; and TRT2: LP diet supplemented with 0.01% MSP. At week 7, the final body weight (BW) was higher in CON and TRT2 than in TRT1 (p < 0.05). During weeks 1–3, the feed conversion ratio (FCR) was lower in CON than in TRT1 (p < 0.05). In weeks 5–7, average daily gain (ADG) was higher in CON and TRT2 than in TRT1 (p < 0.05), while only CON showed a reduced FCR (p < 0.05). Over the entire trial, ADG was highest in TRT2 (p < 0.05), and FCR was lower in both CON and TRT2 compared with TRT1 (p < 0.01). At the end of the experiment, apparent nitrogen digestibility was significantly improved in CON and TRT2 relative to TRT1 (p < 0.05). Fecal Lactobacillus counts were elevated, and Escherichia coli abundance decreased in TRT2 (p < 0.05), indicating a more favorable profile of selected fecal bacterial indicators. In the N balance trial, six healthy piglets (three barrows and three gilts) per treatment were randomly selected and housed individually in metabolism cages. After a 3-day adaptation, total feces and urine were collected for four days to determine N intake, fecal and urinary N excretion, and N retention. At week 7, DM and N intake were unaffected by dietary treatment, whereas fecal N excretion was lower in TRT2 and CON relative to TRT1 (p < 0.05). Overall, supplementation of MSP in LP diets partially mitigated the adverse effects associated with reduced dietary protein by enhancing feed efficiency and nitrogen utilization, resulting in growth performance comparable to that of piglets fed a normal CP diet. Full article

Deoxynivalenol (DON) is a common mycotoxin in cereal crops such as corn, wheat, and their processed products. It can cause feed refusal and growth retardation in piglets. This study systematically evaluated the effects of dietary exposure to purified DON at low doses of 0.25, 0.5, 1.0, and 2.0 mg/kg on growth performance, blood biochemistry, antioxidant capacity, immune function, intestinal health, and reproductive development in female weaned piglets over a 42-day period. Although dietary exposure to 0.25–2.0 mg/kg of DON did not significantly affect growth performance, it induced subclinical multi-organ toxicity. Notably, decreased platelet count (PLT) at 0.25–2.0 mg/kg and increased serum alanine aminotransferase (ALT) activity at 2.0 mg/kg were observed. DON exposure also impaired antioxidant function with reduced serum total antioxidant capacity (T-AOC) at 0.25–2.0 mg/kg, and elevated malondialdehyde (MDA) content in the jejunum and ileum at 0.5–2.0 mg/kg. Furthermore, at all doses tested (0.25–2.0 mg/kg), DON suppressed anti-inflammatory cytokine interleukin-10 (IL-10) levels in both serum and intestine, reduced duodenal villus height (VH), and decreased serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels. Additionally, histopathological injuries of liver, kidney, duodenum, jejunum, ileum, uterus and ovaries were also observed at doses of 1.0–2.0 mg/kg. In summary, this study confirms the multi-organ toxicity of low-dose DON in piglets. Our findings suggest that DON concentrations in pig feed should be more strictly controlled and highlight the importance of considering subclinical health endpoints, such as oxidative stress markers and immune parameters, in future risk assessments of mycotoxin exposure. Full article

In modern animal production systems, the use of alternative feed sources is essential for reducing production costs, protecting natural resources, and improving meat quality. This study evaluated the impact of a unique silage—one produced by agro-industrial by-products—on the growth, meat quality, and health parameters of finishing pigs. Eighteen pigs (120 days old, 59.47 ± 0.85 kg) were randomly assigned to three dietary treatments containing 0% (A), 5% (B), or 10% (C) silage. After 60 days, blood samples were collected for hematological and biochemical analyses; this was followed by slaughter for the collection of muscle tissues (triceps brachii, external abdominal oblique) and intestinal digesta (ileum, cecum). Final body weight did not differ among groups (124.54 ± 1.51 kg), and meat composition (fat, protein, collagen, and ash) and pH were unaffected (p > 0.05). ALT levels were significantly lower in group C than in A (p = 0.030). In the ileum, Enterobacteriaceae and Enterococci populations decreased, whereas Lactobacilli increased in B and C (p ≤ 0.05). Cecal Enterococci were reduced in both B and C (p ≤ 0.01), with a tendency for increased Lactobacilli in B. Group C showed higher total phenolic content (p ≤ 0.05) and an improved ω-6/ω-3 ratio (15.09 vs. 17.54 in A). The TBARS values did not differ among treatments. Campylobacter jejuni counts were lower in treatment C, and no Salmonella or Listeria monocytogenes were detected in any samples. The meat color of the triceps brachii was redder in treatments B and C (p = 0.001). Overall, the innovative silage increased phenolic content, supported improvements in gut microbiota, and maintained meat quality, indicating its potential as a sustainable feed ingredient. Full article

Background: Individual differences in immune responses to African swine fever virus (ASFV), whether induced by vaccination or natural infection, may be linked to genetic variation in the genes involved in antigen presentation. Methods: A total of nine pigs from the 112-population were selected for RNA-seq analysis. To pinpoint key transcription factors (TFs) regulating gene expression in the lymph nodes, weighted Kendall’s Tau rank correlation analysis was performed to link the TF binding potential with the extent of differential expression of target genes. Results: CD8⁺ T cells expressing a specific epitope of the ASFV p72 protein (ACD8⁺) accounted for 41% of the total CD8⁺ T cells in peripheral blood. A total of 2062 transcripts were identified as differentially expressed across the nine pigs (q-value < 1 × 10⁻⁸). Differential expression levels of the target genes for MECP2, ETS1, ZBTB33, ELK4, and E2F4 were significantly correlated with their TF binding potential (p < 0.05). Six SNPs were identified in the promoter region of ELK4. Analysis of the 112-pig population revealed that SNPs at S.-404A>G and S.-668C>T loci were significantly associated with ACD8⁺ levels (q-value < 0.01). Individuals with the AA genotype at S.-404A>G had significantly higher ACD8⁺ counts compared to those with AG and GG genotypes (q-value < 0.05). At the S.-668C>T locus, ACD8⁺ levels were highest in the CC genotype, followed by CT and TT genotypes, with CC showing notably higher ACD8⁺ counts (q-value < 0.05). Notably, the S.-404A>G site overlaps with potential binding sites for TFs FOXA2, GATAs, and TRPS1, while the S.-668C>T site lies within the binding regions for NR1H3, RARA, VDR, and NR1I3. Conclusion: These mutations may disrupt TFs binding to the ELK4 promoter, potentially reducing ELK4 expression and impairing antigen processing and presentation. Full article

Overdispersion is a major problem in the context of count data analysis, and the classical Poisson regression estimators are, in general, unreliable since they imply that the mean equals its variance. In this article, an event-driven class of average estimators, which is based on the Poisson–inverse Gaussian (P-IG) regression model, is formulated to overcome this shortcoming. P-IG regression is a mixture of Poisson and inverse Gaussian regression that is modeled to deal with the overdispersion that is often found in real data. It approximates such count data by a compound distribution with a heavy-tailed inverse Gaussian component. Suggested estimators are more effective in estimating the population means in situations of overdispersion using auxiliary data in the form of covariates. The design-based framework specifies the statistical properties of proposed estimators with respect to their bias and mean squared error (MSE). To confirm the effectiveness and the strength of the suggested methodology, a reasonable amount of simulations and real-data applications are carried out, contrasting it with customary Poisson-based estimators. The results indicate that the P-IG-based estimators are superior over their counterparts. The study provides a statistically valid and practically useful breakthrough in survey sampling and count data regression that can provide researchers and practitioners with a strong alternative to classical Poisson-regression-based mean estimator procedures. Full article

Foetal growth restriction (FGR) is commonly caused by placental insufficiency and increases the risk of perinatal morbidity and mortality. The developing brain is vulnerable to FGR, which can result in adverse long-term neurodevelopmental outcomes. Newborn pigs with spontaneous FGR (<10th centile body weight) and normally grown (NG) littermates were reared to postnatal day 10 (P10; n = 8 FGR and n = 9 NG). Neuropathology was assessed in the prefrontal cortex (PFC) and basal ganglia (BG), which play a key role in cognitive and motor functions. FGR piglets show decreased neuronal count (NeuN) and structural integrity (MAP2) associated with increased apoptotic activity (Casp-3 and -9) in the PFC and BG. Hypomyelination was consistently observed in the white matter of the FGR brain. There was increased microglial activation (Iba-1) and GFAP-positive astrocytes in both the grey and white matter of the PFC and BG, along with increased apoptotic activity in the FGR brain. These findings suggest that the FGR piglet brain shows impaired grey and white matter associated with increased apoptosis in the PFC and BG that persists at P10. Increased glial activation and apoptotic astrocytes may impact neuronal survival and potentially contribute to adverse long-term neurodevelopmental outcomes, highlighting the need for targeted therapeutic interventions to promote effective brain repair in infants with FGR. Full article

The metabolic capacity of swine caecum-derived Lactobacillus spp. to biotransform mycotoxins presents promising potential as a host-probiotic strategy to improve pig health and support host-targeted probiotic research. In the present study, Lactobacillus spp. isolated from the pig caecum were examined for their ability to detoxify fumonisin B1 (FB1) in vitro. Three experimental groups were established (i) Control 1 (C1: buffer + caecal chyme), (ii) Control 2 (C2: buffer + FB1), (iii) Experimental group (E: buffer + caecal chyme + FB1), each with 12 replicates per group (4 replicates per time point 0, 24, and 48 h). Quantitative polymerase chain reaction (qPCR) was used to determine bacterial abundance, while fumonisin B1 (FB1) and its hydrolyzed product (HFB1, Hydrolyzed Fumonisin B1), were quantified using liquid chromatography–mass spectrometry (LC-MS). Group E showed a significant increase in Lactobacillus spp. abundance (p < 0.001), indicating a selective microbial response to FB1 exposure. In contrast, total bacterial counts did not differ significantly between C1 and E (p = 0.35), suggesting that the proliferation of Lactobacillus was the main microbiological outcome supporting the host–probiotic hypothesis. Principal component analysis (PCA) revealed distinct microbial clustering, explaining 97.3% of the variance. Compared to C2, FB1 levels in group E were significantly reduced at 24 and 48 h, while HFB1 conversion rates increased from 47.1% to 56.5%. The study identified Lactobacillus pontis, Lactobacillus amylovorus, and Lactobacillus ultunensis as promising host-associated probiotics, with potential application as feed additives to mitigate mycotoxin effects in pigs. These findings warrant further in vivo validation. Full article

The lossof ear tags in breeding pigs can lead to the loss or confusion of individual identity information. Timely and accurate detection, along with continuous tracking of breeding pigs that have lost their ear tags, is crucial for improving the precision of farm management. However, considering the real-time requirements for the detection of ear tag-lost breeding pigs, coupled with tracking challenges such as similar appearances, clustered occlusion, and rapid movements of breeding pigs, this paper proposed a dual-view synergistic method for detecting ear tag-lost breeding pigs and tracking individuals. First, a lightweight ear tag loss detector was developed by combining the Cascade-TagLossDetector with a channel pruning algorithm. Second, a synergistic architecture was designed that integrates a localized top-down view with a panoramic oblique view, where the detection results of ear tag-lost breeding pigs from the localized top-down view were mapped to the panoramic oblique view for precise localization. Finally, an enhanced tracker incorporating Motion Attention was proposed to continuously track the localized ear tag-lost breeding pigs. Experimental results indicated that, during the ear tag loss detection stage for breeding pigs, the pruned detector achieved a mean average precision of 94.03% for bounding box detection and 90.16% for instance segmentation, with a parameter count of 28.04 million and a detection speed of 37.71 fps. Compared to the unpruned model, the parameter count was reduced by 20.93 million, and the detection speed increased by 12.38 fps while maintaining detection accuracy. In the tracking stage, the success rate, normalized precision, and precision of the proposed tracker reached 86.91%, 92.68%, and 89.74%, respectively, representing improvements of 4.39, 3.22, and 4.77 percentage points, respectively, compared to the baseline model. These results validated the advantages of the proposed method in terms of detection timeliness, tracking continuity, and feasibility of deployment on edge devices, providing significant reference value for managing livestock identity in breeding farms. Full article

Current consumer trends for meat production with reduced antibiotic use constitute huge challenges in animal farming. Using indigenous raw materials such as aromatic or medicinal plants or their extracts could positively affect or retain animals’ health. The present study aimed to evaluate the effects of medicinal plant extracts and essential oils on pig performance parameters, health indices and meat quality. A phytobiotic mixture (PM) consisting of oregano (Origanum vulgare subsp. hirtum) essential oil, rock samphire (Crithmum maritimum L.) essential oil, garlic flour (Allium sativum L.) and false flax flour (Camelina sativa L. Crantz) was used in pig diets, containing in the experimental trials two different proportions of the oregano essential oil (200 mL/t of feed vs. 400 mL/t of feed). Three groups of weaned pigs were fed either the control diet (CONT) or one of the enriched diets (PM-A or PM-B, 2 g/kg). After a 43-day feeding period, at 77 days of age, blood was taken from the jugular vein for biochemical and hematological tests, and eight pigs were humanely slaughtered. A microbiological analysis of intestinal digesta from the ileum and caecum was conducted. Additionally, meat tissue cuts (biceps femoris, external abdominal and triceps brachii) were collected for a chemical analysis, fatty acid lipid profile and oxidative stability testing. The statistical analysis revealed no differences (p > 0.05) in the body weights and growth rates among the groups. An increase (p < 0.05) in total aerobic bacteria was detected in the ileum of group PM-A, while Escherichia coli (E. coli) counts were reduced (p < 0.05) in group PM-B. In the caecum, reductions in Enterobacteriaceae and Lactobacillaceae counts were observed in groups PM-A and PM-B. Concentrations of malondialdehyde (MDA) as an indicator of lipid peroxidation were significantly reduced (p < 0.05) in triceps brachii and biceps femoris for both groups PM-A and PM-B (day 0). A reduction (p < 0.05) in MDA was noticed in triceps brachii and external abdominal meat samples (day 7) for groups PM-A and PM-B. In addition, the fatty acid profile of the meat lipids (ΣPUFA, h/H and PUFA/SFA ratios) was positively modified (p < 0.05) in the ham and belly cuts. The addition of the PM significantly (p < 0.05) affected the redness of the ham and shoulder meat (a* value increased), the yellowness of only the ham (b* value decreased) and the lightness of both belly (L* value increased) and ham samples (L* value decreased). The meat proximate analysis, as well as hematological and biochemical parameters, did not identify any differences (p > 0.05) between the groups. In conclusion, the two investigated mixtures could be used in weaned pigs’ diets, with positive results in intestinal microbial modulation, oxidative stability, fatty acid profile and color characteristics of the pork meat produced. Full article

Accurate, real-time, and cost-effective detection and counting of pre-weaning piglets are critical for improving piglet survival rates. However, achieving this remains technically challenging due to high computational demands, frequent occlusion, social behaviors, and cluttered backgrounds in commercial farming environments. To address these challenges, this study proposes a lightweight and attention-enhanced piglet detection and counting network based on an improved YOLOv8n architecture. The design includes three key innovations: (i) the standard C2f modules in the backbone were replaced with an efficient novel Multi-Scale Spatial Pyramid Attention (MSPA) module to enhance the multi-scale feature representation while a maintaining low computational cost; (ii) an improved Gather-and-Distribute (GD) mechanism was incorporated into the neck to facilitate feature fusion and accelerate inference; and (iii) the detection head and the sample assignment strategy were optimized to align the classification and localization tasks better, thereby improving the overall performance. Experiments on the custom dataset demonstrated the model’s superiority over state-of-the-art counterparts, achieving 88.5% precision and a 93.8% $mA{P}_{0.5}$. Furthermore, ablation studies showed that the model reduced the parameters, floating point operations (FLOPs), and model size by 58.45%, 46.91% and 56.45% compared to those of the baseline YOLOv8n, respectively, while achieving a 2.6% improvement in the detection precision and a 4.41% reduction in the counting MAE. The trained model was deployed on a Raspberry Pi 4B with $ncnn$ to verify the effectiveness of the lightweight design, reaching an average inference speed of <87 ms per image. These findings confirm that the proposed method offers a practical, scalable solution for intelligent pig farming, combining a high accuracy, efficiency, and real-time performance in resource-limited environments. Full article

The assessment of pig welfare and health at abattoirs is crucial for ensuring both animal well-being and food safety. Traditional assessment methods often rely on human observation, which is time-consuming, subjective, and difficult to scale in high-throughput facilities. This systematic review addresses a crucial gap by identifying and evaluating non-invasive human-free diagnostic methods applicable in commercial settings. Following PRISMA guidelines, a total of 102 articles met the inclusion criteria. Thirteen distinct methods were identified and classified into three categories: biological sample analysis (5 methods; n = 80 articles), imaging and computer vision systems (4 methods; n = 19), and physiological and other sensors (4 methods; n = 24). Some articles assessed more than one method and are therefore counted in multiple categories. While no method achieved both high implementation and practicality, blood analysis for glucose and lactate, convolutional neural networks for lesion detection, and automated camera-based systems emerged as the most promising for practical integration into the slaughter flowline. Most techniques still face challenges related to automation, operator independence, and standardisation. Overall, this review highlights the growing potential of non-invasive methods in pig welfare evaluation and underscores the need for continued development and validation to facilitate their adoption into routine abattoir practices. Full article

An adult female Eurasian otter (Lutra lutra), introduced with another subject in the National Park of Gran Paradiso (Aosta Valley Region, Italy), was found dead. The necropsy found a mass involving mainly the gallbladder walls with other multicentric masses in the liver and pancreas. In addition to these, through the histological examination, other nodules were detected in the pancreas, with structure similar to gallbladder neoplasm. Histopathology diagnosed it as neoplasia composed of epithelioid cells, forming lobules of tubules and pseudoacini, with a very low mitotic count, discrete cellular pleomorphism, and prominent fibrous stroma. Neoplastic cells demonstrated positive immunoreactivity for cytokeratin and negative immunoreactivity for S100. Gross and histologic lesions and immunohistochemical findings were consistent with a primary gallbladder carcinoma (GBC) of metastatic type. GBC is a rare neoplasm in both humans and animals, sometimes associated with cholelithiasis and cholecystitis, with few reports in the veterinary literature especially in cattle, pigs, dogs and cats. To the best of our knowledge, this is the first report of GBC in a Eurasian otter. Full article