Search Results (572)

Autophagy is a self-degradative homeostatic mechanism that plays an important role in tumor viability, metabolic reprogramming, and drug resistance. Circulating tumor DNA (ctDNA) is fragmented DNA that comes from dying tumor cells and leaks out into the blood stream. ctDNA can now be measured through blood tests and is a non-invasive way to identify cancer. ctDNA has shown promise for early detection of cancer, prognosis, and monitoring treatment response in real time. There is emerging mechanistic evidence suggesting a potential relationship between autophagy and ctDNA dynamics which has been discussed as a new autophagy–ctDNA axis. Autophagy can affect ctDNA levels by promoting or suppressing apoptosis and necrosis of tumor cells. When autophagy is cytoprotective, less DNA would be shed into the bloodstream. When autophagy is inhibited or defective, more DNA would be released because of increased genomic instability. Stressors found within the tumor microenvironment (TME) like hypoxia, oxidative stress, and nutrient depletion can also induce autophagy and indirectly affect ctDNA. Targeting autophagy therapeutically with drugs that induce or inhibit autophagy such as chloroquine (CQ) or mechanistic target of rapamycin (mTOR) inhibitors can affect ctDNA concentrations. Although emerging mechanistic evidence suggests a potential relationship between autophagy and ctDNA dynamics, direct clinical studies validating this interaction remain lacking. Therefore, this review presents the autophagy–ctDNA relationship as a hypothetical and exploratory model that warrants further mechanistic and translational investigation in cancer development, therapeutic resistance, and clinical applications. Full article

Glyphosate [N-(phosphonomethyl)glycine] is the most widely used herbicide worldwide, and its environmental persistence has prompted increasing interest in microbial processes that may contribute to its dissipation. This study evaluated a collection of 15 soil-derived actinobacterial strains for plant growth-promoting traits, extracellular enzymatic activities, glyphosate tolerance, and glyphosate removal under nutrient-sufficient and phosphate-starved conditions. Herbicide tolerance evaluated on agar plates was widespread across the collection, with all strains sustaining growth at 10 and 50 g L⁻¹ of glyphosate. Under nutrient-sufficient conditions glyphosate removal remained limited, with maximum values of 16.15 ± 2.08% (Streptomyces sp. Con7.16) and 15.34 ± 2.89% (Streptomyces sp. Z38). In contrast, prior phosphate starvation markedly enhanced removal efficiency, reaching 42.21 ± 3.59% in Streptomyces sp. Z38 and 39.46 ± 1.94% in Streptomyces sp. Con7.16. Transmission electron microscopy coupled with X-ray microanalysis in the selected Streptomyces sp. Z38 revealed starvation-associated depletion of intracellular polyphosphate granules, followed by partial replenishment when glyphosate was supplied as the sole phosphorus source, consistent with indirect evidence of glyphosate-derived phosphorus acquisition. Genome mining of Streptomyces sp. Z38 identified candidate genes potentially consistent with a non-canonical, C-P lyase-independent phosphonate utilization route; however, these assignments are based exclusively on bioinformatic evidence and require experimental validation. Collectively, these findings indicate that phosphate limitation enhances glyphosate removal in the selected actinobacteria, and the physiological and genomic data are consistent with a starvation-triggered shift toward alternative phosphorus scavenging strategies. Because this strain is intended for future phytoremediation applications in glyphosate-contaminated agricultural soils, elucidating the underlying phosphorus dynamics is essential for anticipating its functional behavior and environmental relevance. Full article

Radiotherapy (RT) is a cornerstone of cancer treatment, traditionally recognized for its direct cytotoxic effects via DNA damage. However, emerging evidence highlights RT as a profound modulator of the tumor microenvironment (TME), acting as a “double-edged sword” that greatly influences the success of immune checkpoint inhibitors (ICIs). On the one hand, RT acts like an in situ vaccine, causing immunogenic cell death and activating the cGAS-STING pathway, which leads to dendritic cell maturation, T-cell infiltration, and reactive PD-L1 expression. This effect can turn “cold” tumors into “hot” ones, making them more responsive to immune checkpoint blockade. On the other hand, RT can lead to resistance to ICIs by promoting an immunosuppressive environment, recruiting regulatory T cells, M2 macrophages, and myeloid-derived suppressor cells. This review analyzes the mechanisms behind this immunological duality and assesses how parameters such as dose, fractionation, and particle type (e.g., carbon ion versus photon therapy) can be optimized to enhance immune activation. Lastly, we discuss future strategies that focus on innate immunity and tumor metabolism, showing how targeting nutrient depletion and ferroptosis can break down immunosuppressive barriers and position RT as an essential component of precision immuno-oncology. Full article

The cell density effect, defined as reduced cell-specific productivity above a critical cell density, remains a major limitation in virus manufacturing processes. While medium exchange prior to infection has been reported to mitigate this effect, the role of spent medium during the early phase of infection is poorly understood. Here, we show that spent medium conditioned by high-density HEK293 cultures inhibits infection with recombinant vesicular stomatitis virus (rVSV), even when infection is performed at low cell density. The strength of inhibition increased with the density and conditioning time of the donor culture and resulted in slower replication kinetics, thereby delaying the optimal harvest time and potentially reducing overall yield. Notably, the inhibitory effect was reversible when the virus was added to cells maintained in fresh medium, indicating that inhibition is mediated by the medium rather than intrinsic changes in the cells. We excluded pH effects within 7.1–8.0, nutrient depletion, and lactate/ammonium accumulation as primary causes. Removal of cell debris and extracellular vesicles by filtration (down to 0.02 µm) and size-based retention down to 3 kDa did not restore infection, and AUC indicated no major differences in particle distributions between fresh and conditioned media. Together, our data suggest an unidentified <3 kDa inhibitor in spent medium that partially suppresses rVSV infection and slows replication kinetics. Full article

Soil degradation, nutrient depletion, and persistent weed pressure represent critical challenges in the adoption of sustainable agriculture practices in subtropical organic farming systems. Reliance on conventional inputs threatens long-term soil health and ecosystem resilience, highlighting the need for regenerative alternatives. Cover crops are widely recognized as multifunctional agroecological tools with the capacity to enhance nutrient cycling, perform weed suppression, and improve soil organic matter. To evaluate their effectiveness in South Florida's subtropical climate and organic raised bed systems, a field experiment was conducted as a Randomized Block Design (RBD) at the Florida International University Organic Garden during the 2024 summer season. The six cover crops species that were tested include green gram (Vigna radiata), hibiscus (Hibiscus sabdariffa), sorghum (Sorghum bicolor), soybean (Glycine max), sunn hemp (Crotalaria juncea), and pearl millet (Pennisetum glaucum). Data collected includes plant establishment, biomass accumulation, weed suppression, soil physiochemical properties, and plant nutrient composition. Sorghum and sunn hemp produced the highest fresh and dry biomass, with sorghum achieving the most effective weed suppression with the lowest weed biomass and weed population. Sunn hemp contributed to enhanced nitrogen content in plant tissues, while hibiscus promoted the highest soil P and N concentrations. Pearl millet exhibited the highest total carbon and organic matter content, indicating potential for enhancing soil carbon content and soil fertility. Results show that each cover crop species can provide a specialized or generalized ecosystem service depending on management goals. Full article

Continuous cropping obstacles represent a major constraint in agricultural production, yet the underlying microbial mechanisms remain incompletely understood. This study systematically compared soil physicochemical properties, microbial diversity, community composition, and nutrient-microbe relationships between continuous cropping (CC) and non-continuous cropping (CK) celery rhizospheres using high-throughput sequencing, soil physicochemical analysis, and Mantel tests. The results revealed that CC soils exhibited severe depletion of available potassium (AK, 69.9% decreased) and alkali-hydrolyzable nitrogen (AN, 65.9% decreased), accompanied by a modest but statistically significant accumulation of total phosphorus (TP, 8.0% increased). Strikingly, bacterial and fungal communities displayed diametrically opposed diversity responses: CC significantly reduced bacterial α-diversity (Shannon: 5.66 vs. 6.67, p < 0.01) and richness (ACE: 2018 vs. 2623, p < 0.01), whereas fungal diversity and richness more than doubled under CC (ACE: 619 vs. 296, p < 0.01; Shannon: 4.13 vs. 3.34, p < 0.01). β-diversity analyses (NMDS and ANOSIM) confirmed fundamental community restructuring in CC soils for both microbial domains. At the taxonomic level, CC soils showed significant depletion of beneficial plant growth-promoting rhizobacteria (PGPR), including Bacillus (↓89.3%), Mesobacillus (↓72.8%), and Pseudomonas (↓30.8%), coupled with dramatic enrichment of the phytopathogenic genus Fusarium (10.9-fold increase, 8.81% vs. 0.81%, p < 0.001). LEfSe analysis identified Fusarium, Arrhenia, and Mortierella as specific biomarkers of CC soils, whereas Bacillus, Mesobacillus, Cladosporium, and Alternaria were biomarkers of CK soils. Mantel tests further revealed that CC significantly altered nutrient-microbe coupling relationships, with bacterial communities significantly correlated with TP, AN, and OC, and fungal communities with TP, TK, AP, and AN. Collectively, these findings demonstrate that continuous celery cropping shifts the rhizosphere microbiome from a bacterial-dominated profile associated with beneficial taxa (e.g., Bacillus, Pseudomonas) toward a fungal-enriched profile dominated by the pathogen Fusarium, suggesting a potential transition from a putatively disease-suppressive to a disease-conducive microbial state. Full article

This study investigated the interactive effects of the culture system and carbon source on growth, shoot proliferation, and mineral nutrition dynamics in the in vitro propagation of chestnut. Explants of the ‘Akyüz’ cultivar were used in the Woody Plant Medium. Both plant tissues and culture media were analyzed for Fe, Cu, Mn, Zn, and Mg concentrations. Morphological parameters, nutrient accumulation, and depletion patterns were evaluated. The results demonstrated that the liquid culture system supplemented with sucrose significantly enhanced plant growth, chlorophyll content, callus development, and shoot multiplication. Sucrose treatments promoted higher accumulation of Fe, Cu, Zn, and Mg in plant tissues, whereas glucose treatments resulted in significantly higher Mn accumulation. Correlation and principal component analysis revealed strong positive relationships between growth parameters and Fe, Mg, Cu, and Zn, whereas Mn exhibited significant negative correlations. Among the machine learning models, Support Vector Regression showed the highest predictive performance for plant length (R² = 0.74) and SPAD (R² = 0.87). Nutrient depletion analysis showed substantial reductions in mineral concentrations in all treatments after four weeks. Overall, the combination of liquid culture systems with sucrose provides optimal conditions for chestnut micropropagation by promoting favorable nutrient interactions and minimizing antagonistic effects. Full article

Sugarcane is the leading feedstock for bioethanol in Brazil and worldwide, but its continuous cultivation can degrade soil through nutrient depletion and compaction. Integrating green manures such as Crotalaria and pigeon pea into rotations offers a sustainable way to improve soil structure, water infiltration, and nutrient cycling. When combined with sweet sorghum as a complementary crop, these species can mitigate soil physical constraints and strengthen the resilience of sugar–energy systems under rainfed conditions. This three-year field experiment evaluated the effects of green manure and sweet sorghum rotations on sugarcane yield and sandy-soil physical attributes. The treatments were arranged in a 3 × 2 factorial design with randomized blocks, including two green manures (Crotalaria and pigeon pea) and a fallow control, each combined with or without sweet sorghum rotation. Biometric traits and yields were measured for all crops, and soil physical properties were assessed after the sugarcane cycle. Green manure significantly increased the stalk yield and dry matter of both sweet sorghum and sugarcane. In sugarcane, rotations with Crotalaria and pigeon pea enhanced stalk and dry matter yields by up to 18%, while the highest increase (31%) occurred under the sweet sorghum rotation. Furthermore, green manures improved sandy-soil water retention, increased infiltration rates, and reduced penetration resistance. These results demonstrate that legume–sorghum rotations are an effective and low-input strategy to enhance crop yield and sandy-soil physical properties, contributing to more sustainable bioenergy production under tropical rainfed conditions. Full article

Amino acids are essential nutrients for both tumor growth and immune cell function. Cancer cells actively deplete intracellular and extracellular amino acid pools, and limited amino acid availability in the tumor microenvironment (TME) reinforces immunosuppression. Mitochondria are not merely adenosine triphosphate-producing organelles. Amino acid metabolism within mitochondria contributes to tumor progression and influences immune cell fate and effector function. These effects are mediated through biosynthetic precursor generation for lipid, nucleotide, and polyamine synthesis, maintenance redox homeostasis through glutathione and NAD⁺ metabolism, and regulation of gene expression through aryl hydrocarbon receptor signaling. In this review, we discuss four major mitochondrial amino acid metabolic pathways: glutamine-driven anaplerosis, serine/glycine-dependent one-carbon metabolism, arginine–ornithine metabolism, and tryptophan–kynurenine metabolism. We examine how these pathways are rewired in cancer cells, how they influence immune cell function through direct or mitochondria-associated mechanisms, and how such metabolic reprogramming promotes tumor progression while impairing antitumor immunity. Finally, we consider therapeutic strategies to improve cancer immunotherapy by targeting amino acid metabolism, including mitochondrial metabolic enzymes. This review may help guide the development of more effective metabolic biomarkers and mitochondria-based therapeutic strategies for cancer immunotherapy. Full article

Chloroidium saccharophilum is a resilient green microalga with a broad ecological distribution and an increasing biotechnological interest due to its tolerance of extreme environmental conditions. In this study, a sample of C. saccharophilum from the Laguna Blanca aquifer (Magallanes, southern Chile) was physiologically and phylogenetically characterized. This is the first confirmed evidence of this strain in the Southern Cone. Molecular identification based on ITS rDNA sequencing and ITS2 secondary structure analysis confirmed its taxonomic location, showing high similarity with reference strains and no compensatory base changes. Growth performance was analyzed under controlled laboratory conditions and under outdoor desert cultivation in the Atacama Desert, focusing on temperature, salinity, nutrients limitation, and high solar irradiance operational conditions. The strain exhibited optimal growth at 22 °C under laboratory conditions and demonstrated a strong tolerance to high salinity (150 g L⁻¹ NaCl). Outdoor raceways cultivation revealed a negative relationship between temperatures above 25 °C and biomass accumulation, while nutrients depletion and strong irradiance caused moderate carotenoid accumulation. However, the low amount of carotenoid yields remained constant, even under combined stress conditions. In general, the results highlight the ecological adaptability and the stress tolerance of C. saccharophilum, supporting its potential application in saline bioprocesses and bioremediation. Nevertheless, the limited production of carotenoid synthesis suggests that additional or combined stress strategies will be required to enhance the production of high-value metabolites. This study expands the biogeographical knowledge of C. saccharophilum and provides a physiological baseline for future optimization studies in extreme and Mars-analog environments. Full article

Ocean acidification and warming will alter phytoplankton biomass and composition, yet despite numerous studies, there are few consistent responses on which to base predictions. To determine the responses of chlorophyll-a and phytoplankton size and composition to predicted lower pH (−0.33 to −0.5) alone, and also combined with elevated temperature (+2.5–3.5 °C), two mesocosm experiments were carried out in austral spring and autumn in temperate New Zealand coastal waters. Lower pH alone had no effect on chlorophyll-a in either experiment and, as the treatment pH was lower than the pH minimum recorded in a parallel four-year time series, this lack of response in chlorophyll-a was not attributable to prior in situ exposure. Conversely, chlorophyll-a increased under lower pH and warming in both experiments, with the large (>20 µm) phytoplankton size fraction showing opposing responses under nutrient deplete and replete conditions. Diatom biomass also increased in both treatments when nutrient availability was maintained, with a dominant pennate species Cylindrotheca clostridium emerging. The results highlight the value of contextual time series for experimental interpretation, and also the importance of assessing warming and acidification together using regionally representative nutrient concentrations, for prediction of coastal phytoplankton response to climate change. Full article

Species of Penicillium are among the most important fungal pathogens responsible for postharvest diseases of agricultural crops worldwide. This review provides an overview of five economically important Penicillium spp., namely P. expansum, P. digitatum, P. italicum, P. citrinum, and P. oxalicum. Emphasis is placed on P. expansum, P. digitatum, and P. italicum which are the main causal agents of blue mold and green mold rots in pome fruits and citrus, commodities that dominate global fresh produce trade and long-term storage. While studies on plant-pathogenic Penicillium are mainly focused on these hosts, this review highlights reports of infections in other crops across diverse geographic regions, highlighting the broader host range of these species. The main aspects highlighted include host specificity and diversity, production of mycotoxins and other secondary metabolites, current management and control strategies, and the potential influence of climate change on disease incidence and severity. Understanding the biology and epidemiology of plant-pathogenic Penicillium species is essential, as several species are both pathogens and producers of mycotoxins, leading to quality deterioration and nutrient depletion resulting in economic losses. Full article

Drought stress profoundly affects plant growth and survival, but comparisons of integrated adaptive strategies across multiple tree species remain unclear. In this study, seedlings of Elaeagnus angustifolia (E. angustifolia), Populus euphratica (P. euphratica) and Xanthoceras sorbifolium (X. sorbifolium) were subjected to well-watered (CK), mild (T1), moderate (T2), and severe (T3) drought treatments. Leaf microanatomical traits, non-structural carbohydrates (NSCs), stoichiometric elements, biomass allocation, and key stress indicators were measured. The results showed that P. euphratica seedlings thickened leaves and vascular tissues and accumulated soluble sugars (SSs) and starch (ST) under T1–T2, but under T3, they prioritized root investment (root biomass +26.0%); their antioxidant enzymes were activated only under mild-to-moderate stress and declined under severe stress. E. angustifolia seedlings exhibited moderate leaf structural thickening, sharply increased root biomass (+97.2% under T3) while maintaining stem biomass, continuously elevated activities of superoxide dismutase (SOD) and peroxidase (POD) as well as osmoregulatory substances (soluble protein SP, proline Pro), and showed the lowest malondialdehyde (MDA) content; their leaf carbon (C), nitrogen (N), and phosphorus (P) contents decreased the least, and their stoichiometric ratios remained stable. In contrast, X. sorbifolium seedlings progressively reduced leaf thickness and vascular area, depleted NSC reserves, exhibited unstable antioxidant responses, showed a significant decrease in Pro under severe drought, accumulated the highest MDA, and had the lowest N/P ratio, indicating the strongest nitrogen limitation. These results demonstrate that E. angustifolia combines structural plasticity, efficient nutrient use, robust osmotic adjustment, and sustained antioxidant capacity, conferring the strongest drought tolerance; P. euphratica* shows moderate tolerance through transient structural and carbon investment but suffers under extreme drought; X. sorbifolium has the weakest drought tolerance. Full article

Successive cropping frequently causes a decline in Chinese Fir (Cunninghamia lanceolata) biomass, a problem intricately tied to soil nutrient shifts and microbial processes. This research investigates the mechanisms governing biomass carbon partitioning and soil nutrient shifts in these plantations. This study investigated five Chinese Fir clones (‘ck’, ‘b44’, ‘K13’, ‘F13’, and ‘kt13’) across two cultivation regimes: continuous cropping (second-generation plantation, G2) and first-generation plantation (G1). The focus was on their biomass and soil nutrient status. The results showed that: (1) The biomass of different Chinese Fir clones at 25 years of age decreased significantly with increasing generations of continuous cultivation. Tree height showed no significant differences among clones within the same generation; however, the G2 cultivation significantly inhibited diameter at breast height (DBH). (2) The changes in soil nutrients and microbial activity under different successive generations (G1, G2) was closely linked to the decline in Chinese Fir biomass carbon. Analysis revealed that the decreases in dissolved organic carbon (DOC), dissolved organic nitrogen (DON), and Catalase (CAT) activity were significantly positively correlated with the reduction in biomass carbon. Concurrently, the decrease in soil pH showed a significant negative correlation with microbial biomass carbon (MBC) and Sucrase (SUC) activity. (3) Regarding growth traits, although tree height showed no significant differences among clones within the same generation, DBH was generally and significantly inhibited under G2 cultivation. An exception was the ‘K13’ clone, which remained largely unaffected. In terms of carbon accumulation, G2 cultivation led to a universal decline in biomass carbon across clones; however, the magnitude of reduction in different components (leaf, branch, stem, root) and total biomass carbon varied clone-specifically. Notably, ‘K13’ exhibited the strongest tolerance, with a significantly smaller decrease in tree biomass carbon compared to the other four clones, which showed substantially lower tree carbon stocks across all components relative to G1 plantations. This indicates that successive cropping of Chinese Fir likely constrains the carbon sequestration capacity of plantations by altering soil nutrient properties, thereby suppressing tree DBH growth and biomass carbon accumulation, likely through reduced net primary productivity. Among the five clones, ‘K13’ was the least affected, demonstrating its high potential for adaptation to continuous cultivation. These findings provide implications for sustainable forest management by guiding clone selection to mitigate productivity decline under successive cropping. Full article

Quantifying the effects of land-use changes on soil fertility is essential for agricultural planning, yet long-term analyses combining field and remote sensing data remain scarce in Hungary. This systematic review followed PRISMA 2020 guidelines to assess arable land fertility trends between 2000 and 2020. A comprehensive search of WoS, Scopus, and Google Scholar identified 202 records, with 106 studies meeting inclusion criteria. Eligibility required empirical soil data collected from Hungarian arable lands. Among these, 17% reported declines in SOC, 13% indicated nutrient depletion, 36% observed stable or lost fertility, and 34% documented improvements. Regarding monitoring methods, 41% relied solely on field sampling, 44% applied GIS or spatial analyses, and 15% incorporated remote sensing indices such as NDVI. Evidence revealed spatial–temporal heterogeneity: fertility declines occurred in intensively cultivated regions, while western Transdanubia showed stability. Trends were linked to land-use intensification and intermittent reductions in agricultural area. Integration of remote sensing indices, such as NDVI, with field observations enhanced detection of spatial and temporal patterns. These findings underscore the need for harmonised monitoring frameworks, precision agriculture tools, and predictive modelling to support sustainable soil management. Identifying fertility-decline zones informs policy aligned with the EU Soil Strategy 2030 and supports Hungary’s agricultural resilience. Full article