Search Results (251)

Patients with Liver Hepatocellular carcinoma (LIHC) have a poor prognosis due to late-stage diagnosis and the limited efficacy of drug treatments. Dysregulation of nuclear pore complex (NPC) components, particularly nucleoporins (NUPs), may play a role in tumor progression. However, the specific role of NUP205 in LIHC has not been comprehensively investigated. We evaluated the expression, prognostic significance, epigenetic regulation, microRNA(miRNA) interactions, drug sensitivity, and biological functions of NUP205 in LIHC. Comprehensive bioinformatics analyses were performed using publicly available databases and web-based analysis platforms, including The Cancer Genome Atlas (TCGA), UALCAN, and the Kaplan–Meier Plotter (KM Plotter), among others. In vitro validation was performed using small interfering RNA (siRNA)-mediated knockdown of NUP205 in HepG2 cells, followed by quantitative reverse transcription PCR (RT-qPCR), apoptosis assay and wound-healing assay. NUP205 expression was significantly elevated in patients with LIHC and was associated with advanced clinicopathological features and poor prognosis. Promoter hypomethylation and miRNAs were identified as regulatory mechanisms influencing NUP205 expression. Increased NUP205 levels were associated with resistance to multiple chemotherapeutic agents. NUP205 knockdown significantly reduced messenger RNA (mRNA) expression in HepG2 and PLC/PRF/5 cells, and also reduced the expression of Transmembrane protein 209 (TMEM209) in HepG2 cells and improved sensitivity to doxorubicin. NUP205 expression was consistently associated with adverse clinicopathological features, poor prognosis, and altered drug sensitivity in LIHC. Integrative analyses suggest that NUP205 dysregulation may be linked to epigenetic and miRNA-associated regulatory mechanisms. These findings support NUP205 as a candidate prognostic biomarker and a potential regulatory factor in LIHC, warranting further mechanistic and protein-level validation. Further research is necessary to fully elucidate its underlying mechanisms and potential clinical applications. Full article

Prolonged water injection in unconsolidated sandstone reservoirs can induce pore rearrangement and modify flow pathways, thereby affecting reservoir performance. However, quantitative characterization of pore evolution in both temporal and spatial dimensions remains limited. This study investigates the mechanisms of pore-structure evolution during extended injection through a series of multi-scale experiments. Scanning electron microscopy and X-ray diffraction analyses were employed to compare mineral composition and microstructural characteristics before and after injection, while in situ nuclear magnetic resonance (NMR) monitoring captured the dynamic evolution process, enabling pore-size classification from T₂ spectra and fractal assessment of structural complexity. Segmented NMR measurements at different distances further resolved spatial heterogeneity. The results show that prolonged water injection reduced permeability by 10.4–32.1%, whereas porosity exhibited only minor variation, indicating that the decline in flow capacity is primarily controlled by pore–throat structural adjustment rather than pore volume loss. Mineralogical redistribution and fine-particle migration decreased the median pore radius by 21.5–51.8% and the micropore fractal dimension by 23.8–76.5%, with stronger responses observed at higher permeabilities, while meso- and macropore fractal dimensions remained nearly unchanged, indicating preferential modification of micropores with preservation of the main connected flow framework. Consistently, NMR responses reveal pronounced spatial heterogeneity along the flow direction. The NMR signal changes at the injection end were 11.2–18.4% and 7.7–21.7% during the early and intermediate stages, respectively, both exceeding those at the distal end (2.9–12.4% and 1.9–17.1%). These results indicate a downstream-attenuating structural modification gradient. The findings provide new insights into pore-structure evolution during prolonged water injection and offer a scientific basis for optimizing water-injection strategies in unconsolidated sandstone reservoirs. Full article

In the context of the “dual-carbon” targets and the development of green building materials, lightweight mortar has attracted considerable attention, owing to its low density and excellent thermal insulation properties. However, lightweight aggregates, such as vitrified microspheres, while effectively reducing mortar density, exhibit high porosity and weak interfacial bonding, which compromise mechanical performance. To address this issue, this study introduces sugarcane bagasse fiber (SBF) as a reinforcing material, with contents of 0%, 0.4%, 0.8%, 1.2%, and 1.6%. The effects of SBF on physical properties (consistency, density, water absorption) and mechanical properties (compressive strength, flexural strength, and tensile bond strength) were systematically evaluated. Furthermore, low-field nuclear magnetic resonance (LF-NMR) and scanning electron microscopy (SEM) were employed to analyze pore structure and interfacial transition zone (ITZ) characteristics at multiple scales. The results indicate that: (1) at low contents (0.4–0.8%), SBF was uniformly dispersed, improving matrix compactness; (2) compared with the control group, the 28-day compressive, flexural, and tensile bond strengths increased by 7.1%, 13.1%, and 25%, respectively; (3) NMR analysis revealed that the incorporation of SBF significantly increased the proportion of capillary pores, reduced total porosity, and enhanced mortar compactness, thereby improving mechanical strength; (4) fractal dimension analysis showed that contents of 0.4% and 0.8% increased structural complexity while reducing pore connectivity, leading to higher compressive strength; (5) SEM observations further demonstrated that the fibers provided bridging and anchoring effects within the ITZ, promoted the deposition of hydration products, and enhanced interfacial compactness. Full article

Nuclear mechanics and mechanotransduction are involved in the migration and invasion process, such as those in which the cells need to deform themselves to pass through constrictions. Specifically, properties like nuclear softness, viscoelasticity, plasticity (like nuclear pore complexes) and deformability are critical in cancer and its malignant progression. The nucleus represents a physical barrier for the migration and invasion in dense 3D extracellular matrix (ECM) scaffolds. Therefore, the deformability of the nucleus seems to determine the migration limit in circumstances where the enzymatic remodeling of the surroundings is impaired. There are still significant knowledge gaps regarding effects of nuclear deformation during cancer dissemination. It seems that nuclear deformation can alter gene transcription, induce alternative splicing processes, impact nuclear envelope rupture, nuclear pore complex dilatation, damage the DNA, and increase the genomic instability. These mechanically induced alterations can in turn impact the migratory behavior of the cancer cells. The stiffness of the nucleus relies on the condensation of chromatin, and the nuclear lamina, which consists of a network of intermediate filaments underneath the nuclear envelope. All of this is discussed in the review and it is argued that nuclear deformability is universally found in various cancer types. Another focus is placed on the nuclear envelope proteins like emerin, and the SUN-KASH complex and how they contribute to the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, which consequently couples the nucleus and the cytoskeleton. It is argued that this connection is crucial for force transmission, which governs nuclear stiffness dynamically, depending on the force applied. In this review, recent findings are described that couple ECM-induced nuclear mechanosensing and mechanotransduction with the migration and invasion of cancer cells. Moreover, it is suspected that changes in the mechanosensory characteristics of the cell nucleus could play a pivotal part in the malignancy of cancer cells and the heterogeneity of tumors. Finally, it is discussed what impact the individual elements of the nucleus offer to mechanically alter cellular migration and invasion in cancer and its malignant progression. Full article

To address the critical challenge of drilling fluid invasion in deep coalbed methane (CBM) reservoirs, this study provides novel insight into the micro-scale sealing mechanism and pore structure evolution by leveraging Low-Field Nuclear Magnetic Resonance (LF-NMR) as a quantitative probe. Unlike traditional macroscopic evaluations, we utilized dynamic NMR T₂ spectral analysis to decipher the synergistic behavior of a proposed “Bridging–Filling–Densifying” ternary sealing system, which integrates a nano-plugging agent, micro-fillers, and size-matched skeletal agents. The results demonstrate a significant improvement in sealing efficiency. The optimized hierarchical architecture reduced the NMR signal intensity of the invaded cores by over 99.8% under a differential pressure of 10 MPa, effectively eliminating fluid invasion channels. Crucially, the study reveals that while multi-scale particle size matching is the precondition for sealing, the mechanical rigidity of the skeletal particles is the determinant for maintaining filter cake integrity against high-pressure deformation. These findings elucidate the transition from a “macropore-dominated” structure to a “zero-detectable” sealed state, establishing a robust theoretical framework for designing non-damaging drilling fluids tailored to the complex geomechanics of deep CBM exploration. Full article

Tight sandstone reservoirs are characterized by highly heterogeneous pore structures, in which multiscale pore–throat systems jointly control the shapes of capillary pressure curves and relative permeability, thereby exerting a fundamental influence on water production behavior and the overall development performance of gas reservoirs. The Ordos Basin is generally characterized by the development of tight sandstone. The tight sandstones exhibit porosities of 2–13% and permeabilities of 0.01–10 × 10⁻³ μm². To quantitatively elucidate the controlling mechanisms of multiscale pore structure on capillary pressure curve morphology and relative permeability, this study systematically investigates the fractal and multifractal characteristics of pore structures in tight sandstones based on high-pressure mercury intrusion (MICP) and nuclear magnetic resonance (NMR) experimental data, and establishes a quantitative relationship between fractal parameters and the capillary pressure curve shape parameter λ. First, capillary pressure curves were fitted using the Brooks–Corey model within the effective saturation interval to extract the shape parameter λ, which characterizes the concentration degree of pore-size distribution and the drainage behavior. Subsequently, based on NMR T₂ spectra, the small-pore fractal dimension D₁, large-pore fractal dimension D₂, and the multifractal singularity spectrum width Δα were extracted to quantitatively describe the geometric complexity of pore structures at different scales. On this basis, the correlations between λ and D₁, D₂, and Δα were systematically analyzed, and the predictive performance of λ under different parameter combinations was compared. The results indicate that: (1) the pore structures of tight sandstones exhibit pronounced fractal and multifractal characteristics at the NMR T₂ scale, with significant differences among samples; (2) λ shows an overall negative correlation with fractal parameters, among which the correlations with the large-pore fractal dimension D₂ and the multifractal spectrum width Δα are the most significant; (3) compared with models using a single fractal dimension, the multiparameter model incorporating Δα provides a more comprehensive characterization of multiscale pore heterogeneity, leading to a substantial improvement in the accuracy and stability of λ prediction; and (4) λ exerts a clear control on the shape of relative permeability curves, where a larger λ corresponds to earlier initiation and forward-shifted rising segments of water-phase flow, while a smaller λ results in overall flatter relative permeability curves. From the perspectives of fractal and multifractal theory, this study establishes an intrinsic linkage among pore structure, capillary pressure curve shape parameters, and relative permeability, providing a novel quantitative framework for constraining relative permeability curve morphology in tight sandstones under conditions where systematic relative permeability experiments are unavailable. Full article

Variations in the groundwater chemical environment are a critical factor affecting the mechanical property degradation and structural alteration of coal measure strata. Addressing the engineering challenges commonly encountered in coal mining areas of Northwest China, where groundwater with varying pH leads to difficulties in controlling surrounding rock in underground spaces, this study established a comprehensive experimental methodology integrating mechanical loading, nuclear magnetic resonance (NMR) quantitative pore analysis, and scanning electron microscopy (SEM) microstructural characterization. The study revealed the mechanical degradation mechanisms and microstructural evolution characteristics of coal measure coarse sandstone under groundwater environments with different pH values (6–10). With prolonged immersion time, the peak strength and elastic modulus of the coarse sandstone exhibited exponential decay across all pH environments. NMR analysis revealed that the porosity evolved through a path of “increase–decrease–re-increase,” while the macroscopic mechanical failure mode shifted from brittle to brittle-ductile and finally to ductile characteristics. Micropores continuously transformed into medium and large pores, and the macroscopic failure mode exhibited a transition from brittle to brittle-ductile. The findings indicate that groundwater with varying acidity/alkalinity systematically alters the integrity and load-bearing capacity of coal measure coarse sandstone through the complex mechanism of “mineral dissolution (acidic H⁺ corrosion, alkaline OH⁻ hydrolysis)—structural damage—pore/fracture evolution—mechanical degradation.” This mechanism not only reveals the essence of progressive rock damage in weak acid to moderately strong alkaline environments but also provides important insights for the integrity, sealing capacity, and permeability modification of various underground engineering applications, such as CO₂ geological storage, unconventional natural gas development, and underground space utilization. Full article

Natural gas hydrate (NGH) deposits represent a vast and clean energy source. However, sustainable gas production from these resources remains an unsolved technical problem due to potential geohazards and climate challenges. A critical issue in this regard is the difficulty of obtaining in situ samples, which are essential for detailed laboratory studies of NGH’s geomechanical and chemical behavior for safe and green gas production after hydrate dissociation. Currently, the retrieval of representative samples from NGH reservoirs is hindered by significant technological limitations and high costs. Consequently, laboratory-synthesized gas hydrate-bearing sediment (HBS) samples are crucial for controlled research purposes and validating numerical simulation models and are used in the majority of research studies. With this in mind and considering the complexity of synthesizing HBS samples, this study comprehensively reviews different methods of synthesizing gas hydrates in porous media, including excess-gas, excess-water, dissolved-gas, spray, bubble injection, and hybrid techniques. Each method produces distinct hydrate morphologies (e.g., pore-filling, cementing, grain-coating, etc.) and saturation levels, with trade-offs in speed, uniformity, reproducibility, and ease of control. Furthermore, the current review details the synergic application of non-invasive characterization techniques, i.e., X-ray Computed Tomography (CT) and Nuclear Magnetic Resonance (NMR), in studying gas hydrates. CT provides high-resolution three-dimensional (3D) structural images of pore geometry and hydrate distribution, while NMR/MRI (Magnetic Resonance Imaging) quantifies fluid saturations and tracks hydrate formation/dissociation dynamics in real time. The synergistic use of CT and NMR offers a powerful multimodal approach, overcoming individual limitations such as CT’s poor hydrate–water contrast detection and NMR’s indirect hydrate inference, which could help in the sustainable synthesis of particular hydrate morphologies. Finally, the critical analysis of current technological challenges or gaps and also the emerging trends and future directions in the study of HBS, including advanced imaging techniques, AI-assisted analysis, and standardization efforts, etc., are discussed. It was found that the selection of the most appropriate method for natural gas hydrate synthesis is mostly task-specific, and the emerging technologies have facilitated the synthesis of HBS samples with more precise control of morphology, saturation, etc. This review provides the required insights for sustainable synthesis and characterization of hydrate-bearing sediments samples and serves sustainable gas production from natural gas hydrate reservoirs. Full article

Upper E_s4 lacustrine calcareous shale in the Dongying Depression is characterized by strong pore–throat heterogeneity that limits shale-oil producibility. This study quantifies multiscale pore–throat complexity using high-pressure mercury intrusion-based fractal analysis (segmented fractal dimensions D₁–D₃ and a weighted comprehensive fractal dimension, D_c) and evaluates its control on oil occurrence and mobilization using low-field 2D NMR (T₁–T₂) and confocal microscopy before and after high-temperature, high-pressure spontaneous imbibition. Reservoirs show clear scale segmentation, with micropore fractality governing quality differentiation. Imbibition promotes desorption and redistribution from adsorbed to free oil, but effective mobilization is primarily controlled by pore–fracture connectivity: samples with well-connected networks can mobilize both adsorbed and free oil efficiently, whereas poorly connected systems exhibit desorption without effective production, implying that thermal stimulation alone is insufficient without fracture-assisted flow pathways. Movable-oil saturation decreases systematically with increasing D_c, indicating that higher roughness and tortuosity intensify capillary retention and Jamin trapping. D_c provides an actionable criterion for sweet-spot ranking and for designing stimulation–imbibition coupling and water-based EOR strategies in lacustrine calcareous shale-oil reservoirs. Full article

Integrating enhanced oil recovery (EOR) with geological carbon storage presents a dual-strategy solution for sustainable hydrocarbon production and greenhouse gas emission mitigation. CO₂ flooding, particularly under miscible conditions, is a pivotal technology in this endeavor. This study employs advanced in situ nuclear magnetic resonance (NMR) imaging to visually and quantitatively investigate the pore-scale mechanisms of CO₂ flooding in fractured carbonate rocks from a Kazakhstan oilfield. By establishing a novel correlation between NMR data and pore throat size distribution, we quantify the lower limit of pore throat mobilization—a key parameter for evaluating storage and displacement efficiency. Results show that miscible CO₂ flooding significantly reduces this limit to the submicron scale (0.1 μm) in matrix rocks, dramatically improving oil recovery from small pores. However, fracture networks dominate fluid flow, leading to early gas breakthrough and severely limiting CO₂ penetration and miscible displacement in the matrix. The study provides pore-scale insights for optimizing CO₂ injection strategies to maximize both hydrocarbon recovery and CO₂ storage potential in complex carbonate formations. The study elucidates the microscopic mobilization mechanisms and remaining oil distribution patterns during CO₂ flooding in volatile reservoirs. Moreover, it represents an environmentally friendly methodology for mitigating greenhouse gas emissions. Full article

HIV-1 entry into host cells culminates in integration of the reverse transcribed double-stranded viral DNA into host genes. Several lines of evidence suggest that intact, or nearly intact, HIV-1 cores—large, ~60 nm-wide structures—pass through the nuclear pore complex (NPC), and that this passage is associated with pore remodeling. Cryo-electron tomography studies support the dynamic nature of NPCs and their regulation by cytoskeleton and ATP-dependent processes. To explore NPC remodeling, we used super-resolution Stochastic Optical Reconstruction Microscopy (STORM) of U2OS cells endogenously expressing nucleoporin 96 tagged with SNAP. Single-molecule localization imaging and computational averaging resolved 8-fold symmetric nuclear pores with an average radius of ~51 nm. Depletion of cellular ATP using sodium azide or antimycin A, previously reported to reduce the size of yeast NPCs, did not significantly alter the nuclear pore radius in U2OS cells. Similarly, stressing the nuclear envelope by hypotonic or hypertonic conditions failed to induce detectable expansion or contraction of NPCs. These results indicate that the NPCs in U2OS cells do not respond to ATP depletion nor mechanical stresses on changes in pore morphology that can be resolved by STORM. Since these cells are infectable by HIV-1, we surmise that direct multivalent interactions between HIV-1 capsid and phenylalanine-glycine nucleoporins lining the pore’s interior drive the core penetration into the nucleus and the associated changes in the pore structure. Full article

The Wufeng–Longmaxi (WF–LMX) shale gas reservoirs at depths > 3500 m in the Lu203–Yang101 well block, southern Sichuan Basin, possess great exploration potential, but their reservoir characteristics and high-production mechanisms remain unclear. In this study, we employed multi-scale analyses—including core geochemistry, X-ray diffraction (XRD), scanning electron microscopy (SEM), low-pressure N₂ adsorption, and nuclear magnetic resonance (NMR)—to characterize the macro- and micro-scale characteristics of these deep shales. By comparing with shallower shales in adjacent areas, we investigated differences in pore structure between deep and shallow shales and the main controlling factors for high gas-well productivity. The results show that the Long 11 sub-member shales are rich in organic matter, with total organic carbon (TOC) content decreasing upward. The mineral composition is dominated by quartz (averaging ~51%), which slightly decreases upward, while clay content increases upward. Porosity ranges from 1% to 7%; the Long11-1-3 sublayers average 4–6%, locally >6%. Gas content correlates closely with TOC and porosity, highest in the Long11-1 sublayer (6–10 m³/t) and decreasing upward, and the central part of the study area has higher gas content than adjacent areas. The micro-pore structure exhibits pronounced stratigraphic differences: the WF Formation top and Long11-1 and Long11-3 sublayers are dominated by connected round or bubble-like organic pores (50–100 nm), whereas the Long11-2 and Long11-4 sublayers contain mainly smaller isolated organic pores (5–50 nm). Compared to shallow shales nearby, the deep shales have a slightly lower proportion of organic pores, smaller pore sizes with more isolated pores, inorganic pores of mainly intraparticle types, and more developed microfractures, confirming that greater burial depth leads to a more complex pore structure. Type I high-quality reservoirs are primarily distributed from the top of the WF Formation to the Long11-3 sublayer, with a thickness of 15.6–38.5 m and a continuous thickness of 13–23 m. The Lu206–Yang101 area has the thickest high-quality reservoir, with a cumulative thickness of Type I + II exceeding 60 m. Shale gas-well high productivity is jointly controlled by multiple factors: an oxygen-depleted, stagnant deep-shelf environment, with deposited organic-rich, biogenic siliceous shales providing the material basis for high yields; abnormally high pore-fluid pressure with preserved abundant large organic pores and increased free gas content; and effective multi-stage massive fracturing connecting a greater reservoir volume, which is the key to achieving high gas-well production. This study provides a scientific basis for evaluating deep marine shale gas reservoirs in southern Sichuan and understanding the enrichment patterns for high productivity. Full article

The Chang 7 shale oil reservoirs of the Yanchang Formation in the Heishui Area of the Ordos Basin display typical tight sandstone characteristics, marked by complex microscopic pore structures and limited flow capacity, which severely constrain efficient development. Using a suite of laboratory techniques—including nuclear magnetic resonance, mercury intrusion porosimetry, oil–water relative permeability, spontaneous imbibition experiments, scanning electron microscopy, and thin section analysis—this study systematically characterizes representative tight sandstone samples and examines the microscopic distribution of remaining oil, flow behavior, and their controlling factors. Results indicate that residual oil is mainly stored in nanoscale micropores, whereas movable fluids are predominantly concentrated in medium to large pores. The bimodal or trimodal T₂ spectra reflect the presence of multiscale pore–fracture systems. Spontaneous imbibition and relative permeability experiments reveal low displacement efficiency (average 41.07%), with flow behavior controlled by capillary forces and imbibition rates exhibiting a three-stage pattern. The primary factors influencing movable fluid distribution include mineral composition (quartz, feldspar, lithic fragments), pore–throat structure (pore size, sorting, displacement pressure), physical properties (porosity, permeability), and heterogeneity (fractal dimension). High quartz and illite contents enhance effective flow pathways, whereas lithic fragments and swelling clay minerals significantly impede fluid migration. Overall, this study clarifies the coupled “lithology–pore–flow” control mechanism, providing a theoretical foundation and practical guidance for the fine characterization and efficient development of tight oil reservoirs. The findings can directly guide the optimization of hydraulic fracturing and enhanced oil recovery strategies by identifying high-mobility zones and key mineralogical constraints, enabling targeted stimulation and improved recovery in the Chang 7 and analogous tight reservoirs. Full article

The p53 tumor suppressor, a key transcription factor, acts as a cellular stress sensor that regulates hundreds of genes involved in responses to DNA damage, oxidative stress, and ischemia. Through these actions, p53 can arrest cell cycle, initiate DNA repair, or trigger cell death. In addition to its nuclear functions, p53 can translocate to mitochondria to promote apoptosis. Studies using isolated mitochondria have suggested that p53 drives the voltage-dependent anion channel (VDAC1) into high molecular mass complexes to mediate apoptosis. VDAC1 is a central regulator of cellular energy production and metabolism and also an essential player in apoptosis, induced by various apoptotic stimuli and stress conditions. We previously demonstrated that VDAC1 oligomerization, induced by various apoptosis stimuli and stress conditions, forms a large pore that enables cytochrome c release from mitochondria, thereby promoting apoptotic cell death. In this study, we show that p53 interacts with VDAC1, modulates its expression levels, and promotes VDAC1 oligomerization-dependent apoptosis. Using purified proteins, we found that p53 directly binds VDAC1, as revealed by microscale thermophoresis and by experiments using bilayer-reconstituted VDAC1, in which p53 reduced VDAC1 channel conductance. Furthermore, overexpression of p53 in p53-null cells or in cells expressing wild-type p53 increased VDAC1 expression and induced VDAC1 oligomerization even in the absence of apoptotic stimuli. Together, these findings identify VDAC1 as a direct p53 target whose expression, oligomerization, and pro-apoptotic activity are regulated by p53. They also reinforce the central role of VDAC1 oligomerization in apoptosis. Full article

The shale oil reservoir is characterized by ultra-low porosity and permeability and multi-scale strong heterogeneity. During the sampling process of downhole cores, the rocks can easily be affected by drilling fluid contamination, mechanical stress damage, and other factors, altering the original distribution of oil–water and the characteristics of pore structures. Oil removal and oil saturation are critical steps in core pre-treatment, yet the mechanism of its impact on cores has not been systematically studied. This research focuses on oil removal in six cores from the Jimsar shale oil reservoir with different oil saturations. The necessity and effectiveness of the oil removal saturation and its impact on the microstructure of the cores were systematically evaluated by employing nuclear magnetic resonance (NMR), CT scanning, and permeability testing methods. The results indicate that there are significant differences in fluid composition, pore structure, and wettability among downhole cores, making oil removal saturation treatment a necessary prerequisite for subsequent experiments. High-temperature and high-pressure oil removal shows significant effectiveness, with an average core weight reduction of 2.46% and average reduction in T₂ peak area of 73.75%. The efficacy of oil saturation is influenced by the initial pore-throat distribution in the cores. The oil removal process significantly alters petrophysical parameters, with an average increase in porosity of 3.21 times and permeability rising by an average of 2.16 times, although individual variations exist. Microstructural analysis demonstrates that the oil removal process preferentially removes crude oil from larger pores, while residual oil is mainly distributed in smaller pores, indicated by a left shift in T₂ peak values. Meanwhile, high-temperature and high-pressure conditions induce microfracture development, promoting the migration of crude oil into smaller pores. This research reveals the complex impact mechanism of the oil removal saturation process on shale cores, providing a theoretical basis for accurately evaluating shale reservoir characteristics and optimizing experimental design. Full article