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21 pages, 559 KiB  
Review
Interest Flooding Attacks in Named Data Networking and Mitigations: Recent Advances and Challenges
by Simeon Ogunbunmi, Yu Chen, Qi Zhao, Deeraj Nagothu, Sixiao Wei, Genshe Chen and Erik Blasch
Future Internet 2025, 17(8), 357; https://doi.org/10.3390/fi17080357 (registering DOI) - 6 Aug 2025
Abstract
Named Data Networking (NDN) represents a promising Information-Centric Networking architecture that addresses limitations of traditional host-centric Internet protocols by emphasizing content names rather than host addresses for communication. While NDN offers advantages in content distribution, mobility support, and built-in security features, its stateful [...] Read more.
Named Data Networking (NDN) represents a promising Information-Centric Networking architecture that addresses limitations of traditional host-centric Internet protocols by emphasizing content names rather than host addresses for communication. While NDN offers advantages in content distribution, mobility support, and built-in security features, its stateful forwarding plane introduces significant vulnerabilities, particularly Interest Flooding Attacks (IFAs). These IFA attacks exploit the Pending Interest Table (PIT) by injecting malicious interest packets for non-existent or unsatisfiable content, leading to resource exhaustion and denial-of-service attacks against legitimate users. This survey examines research advances in IFA detection and mitigation from 2013 to 2024, analyzing seven relevant published detection and mitigation strategies to provide current insights into this evolving security challenge. We establish a taxonomy of attack variants, including Fake Interest, Unsatisfiable Interest, Interest Loop, and Collusive models, while examining their operational characteristics and network performance impacts. Our analysis categorizes defense mechanisms into five primary approaches: rate-limiting strategies, PIT management techniques, machine learning and artificial intelligence methods, reputation-based systems, and blockchain-enabled solutions. These approaches are evaluated for their effectiveness, computational requirements, and deployment feasibility. The survey extends to domain-specific implementations in resource-constrained environments, examining adaptations for Internet of Things deployments, wireless sensor networks, and high-mobility vehicular scenarios. Five critical research directions are proposed: adaptive defense mechanisms against sophisticated attackers, privacy-preserving detection techniques, real-time optimization for edge computing environments, standardized evaluation frameworks, and hybrid approaches combining multiple mitigation strategies. Full article
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28 pages, 845 KiB  
Review
Circulating Tumor DNA in Prostate Cancer: A Dual Perspective on Early Detection and Advanced Disease Management
by Stepan A. Kopytov, Guzel R. Sagitova, Dmitry Y. Guschin, Vera S. Egorova, Andrei V. Zvyagin and Alexey S. Rzhevskiy
Cancers 2025, 17(15), 2589; https://doi.org/10.3390/cancers17152589 - 6 Aug 2025
Abstract
Prostate cancer (PC) remains a leading cause of malignancy in men worldwide, with current diagnostic methods such as prostate-specific antigen (PSA) testing and tissue biopsies facing limitations in specificity, invasiveness, and ability to capture tumor heterogeneity. Liquid biopsy, especially analysis of circulating tumor [...] Read more.
Prostate cancer (PC) remains a leading cause of malignancy in men worldwide, with current diagnostic methods such as prostate-specific antigen (PSA) testing and tissue biopsies facing limitations in specificity, invasiveness, and ability to capture tumor heterogeneity. Liquid biopsy, especially analysis of circulating tumor DNA (ctDNA), has emerged as a transformative tool for non-invasive detection, real-time monitoring, and treatment selection for PC. This review examines the role of ctDNA in both localized and metastatic PCs, focusing on its utility in early detection, risk stratification, therapy selection, and post-treatment monitoring. In localized PC, ctDNA-based biomarkers, including ctDNA fraction, methylation patterns, fragmentation profiles, and mutations, demonstrate promise in improving diagnostic accuracy and predicting disease recurrence. For metastatic PC, ctDNA analysis provides insights into tumor burden, genomic alterations, and resistance mechanisms, enabling immediate assessment of treatment response and guiding therapeutic decisions. Despite challenges such as the low ctDNA abundance in early-stage disease and the need for standardized protocols, advances in sequencing technologies and multimodal approaches enhance the clinical applicability of ctDNA. Integrating ctDNA with imaging and traditional biomarkers offers a pathway to precision oncology, ultimately improving outcomes. This review underscores the potential of ctDNA to redefine PC management while addressing current limitations and future directions for research and clinical implementation. Full article
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10 pages, 1663 KiB  
Article
First Detection and Molecular Identification of Rhabditis (Rhabditella) axei from the Chinese Red Panda (Ailurus styani)
by Chanjuan Yue, Wanjing Yang, Dunwu Qi, Mei Yang, James Edward Ayala, Yanshan Zhou, Chao Chen, Xiaoyan Su, Rong Hou and Songrui Liu
Pathogens 2025, 14(8), 783; https://doi.org/10.3390/pathogens14080783 - 6 Aug 2025
Abstract
Rhabditis (Rhabditella) axei is a predominantly free-living nematode commonly found in sewage systems and decomposing organic matter. While primarily saprophytic, it has been documented as an opportunistic pathogen in human urinary and gastrointestinal tracts. The Chinese red panda (Ailurus styani [...] Read more.
Rhabditis (Rhabditella) axei is a predominantly free-living nematode commonly found in sewage systems and decomposing organic matter. While primarily saprophytic, it has been documented as an opportunistic pathogen in human urinary and gastrointestinal tracts. The Chinese red panda (Ailurus styani), a rare and protected species in China, has not previously been reported as a host for Rhabditis (Rhabditella) spp. infections. This study reports the first documented occurrence of R. axei in red panda feces, unambiguously confirmed through integrative taxonomic approaches combining morphological and molecular analyses. The nematodes exhibited key morphological features consistent with R. axei, including a cylindrical rhabditiform esophagus, sexually dimorphic tail structures, and diagnostic spicule morphology. Molecular analysis based on 18S-ITS-28S rDNA sequencing confirmed their identity, showing >99% sequence similarity to R. axei reference strains (GenBank: PP135624.1, PP135622.1). Phylogenetic reconstruction using 18S rDNA and ITS rDNA sequences placed the isolate within a well-supported R. axei clade, clearly distinguishing it from related species such as R. blumi and R. brassicae. The findings demonstrate the ecological plasticity of R. axei as a facultative parasite capable of infecting non-traditional hosts and further highlight potential zoonotic risks associated with environmental exposure in captive wildlife populations. Our results emphasize the indispensable role of molecular diagnostics in accurately distinguishing morphologically similar nematodes within the Rhabditidae family, while providing essential baseline data for health monitoring in both in situ and ex situ conservation programs for this endangered species. Full article
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20 pages, 7088 KiB  
Article
SAR Images Despeckling Using Subaperture Decomposition and Non-Local Low-Rank Tensor Approximation
by Xinwei An, Hongcheng Zeng, Zhaohong Li, Wei Yang, Wei Xiong, Yamin Wang and Yanfang Liu
Remote Sens. 2025, 17(15), 2716; https://doi.org/10.3390/rs17152716 - 6 Aug 2025
Abstract
Synthetic aperture radar (SAR) images suffer from speckle noise due to their imaging mechanism, which deteriorates image interpretability and hinders subsequent tasks like target detection and recognition. Traditional denoising methods fall short of the demands for high-quality SAR image processing, and deep learning [...] Read more.
Synthetic aperture radar (SAR) images suffer from speckle noise due to their imaging mechanism, which deteriorates image interpretability and hinders subsequent tasks like target detection and recognition. Traditional denoising methods fall short of the demands for high-quality SAR image processing, and deep learning approaches trained on synthetic datasets exhibit poor generalization because noise-free real SAR images are unattainable. To solve this problem and improve the quality of SAR images, a speckle noise suppression method based on subaperture decomposition and non-local low-rank tensor approximation is proposed. Subaperture decomposition yields azimuth-frame subimages with high global structural similarity, which are modeled as low-rank and formed into a 3D tensor. The tensor is decomposed to derive a low-dimensional orthogonal basis and low-rank representation, followed by non-local denoising and iterative regularization in the low-rank subspace for data reconstruction. Experiments on simulated and real SAR images demonstrate that the proposed method outperforms state-of-the-art techniques in speckle suppression, significantly improving SAR image quality. Full article
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51 pages, 2489 KiB  
Review
Immunomodulatory Effects of Gold Nanoparticles: Impacts on Immune Cells and Mechanisms of Action
by Khadijeh Koushki, Prapannajeet Biswal, Geraldine Vidhya Vijay, Mahvash Sadeghi, Sajad Dehnavi, Ngoc Tuyet Tra, Sai Kumar Samala, Mahdieh Yousefi Taba, Arjun Balaji Vasan, Emily Han, Yuri Mackeyev and Sunil Krishnan
Nanomaterials 2025, 15(15), 1201; https://doi.org/10.3390/nano15151201 - 6 Aug 2025
Abstract
Traditional anti-inflammatory medications—such as corticosteroids, biological agents, and non-steroidal anti-inflammatory drugs—are commonly employed to mitigate inflammation, despite their potential for debilitating side effects. There is a growing need for alternative next-generation therapies for symptomatic, unchecked, and/or detrimental inflammation with more favorable adverse effect [...] Read more.
Traditional anti-inflammatory medications—such as corticosteroids, biological agents, and non-steroidal anti-inflammatory drugs—are commonly employed to mitigate inflammation, despite their potential for debilitating side effects. There is a growing need for alternative next-generation therapies for symptomatic, unchecked, and/or detrimental inflammation with more favorable adverse effect profiles. The long history of use of gold salts as anti-inflammatory agents and the more recent exploration of gold nanoparticle (AuNP) formulations for clinical indications suggest that the targeted delivery of nanoparticles to inflammatory sites may be a promising approach worth investigating. Coupled with peptides that specifically target immune cells, AuNPs could potently counteract inflammation. Here, we provide an overview of the selective infiltration of AuNPs into immune cells and summarize their interactions with and impact on these cells. Additionally, we provide a comprehensive mechanistic summary of how AuNPs exert their anti-inflammatory effects. Full article
(This article belongs to the Special Issue Roadmaps for Nanomaterials in Radiation Therapy)
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24 pages, 11081 KiB  
Article
Quantifying Wildfire Dynamics Through Spatio-Temporal Clustering and Remote Sensing Metrics: The 2023 Quebec Case Study
by Tuğrul Urfalı and Abdurrahman Eymen
Fire 2025, 8(8), 308; https://doi.org/10.3390/fire8080308 - 5 Aug 2025
Abstract
Wildfires have become increasingly frequent and destructive environmental hazards, especially in boreal ecosystems facing prolonged droughts and temperature extremes. This study presents an integrated spatio-temporal framework that combines Spatio-Temporal Density-Based Spatial Clustering of Applications with Noise (ST-DBSCAN), Fire Radiative Power (FRP), and the [...] Read more.
Wildfires have become increasingly frequent and destructive environmental hazards, especially in boreal ecosystems facing prolonged droughts and temperature extremes. This study presents an integrated spatio-temporal framework that combines Spatio-Temporal Density-Based Spatial Clustering of Applications with Noise (ST-DBSCAN), Fire Radiative Power (FRP), and the differenced Normalized Burn Ratio (ΔNBR) to characterize the dynamics and ecological impacts of large-scale wildfires, using the extreme 2023 Quebec fire season as a case study. The analysis of 80,228 VIIRS fire detections resulted in 19 distinct clusters across four fire zones. Validation against the National Burned Area Composite (NBAC) showed high spatial agreement in densely burned areas, with Intersection over Union (IoU) scores reaching 62.6%. Gaussian Process Regression (GPR) revealed significant non-linear relationships between FRP and key fire behavior metrics. Higher mean FRP was associated with both longer durations and greater burn severity. While FRP was also linked to faster spread rates, this relationship varied by zone. Notably, Fire Zone 2 exhibited the most severe ecological impact, with 83.8% of the area classified as high-severity burn. These findings demonstrate the value of integrating spatial clustering, radiative intensity, and post-fire vegetation damage into a unified analytical framework. Unlike traditional methods, this approach enables scalable, hypothesis-driven assessment of fire behavior, supporting improved fire management, ecosystem recovery planning, and climate resilience efforts in fire-prone regions. Full article
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22 pages, 3217 KiB  
Article
A Deep Reinforcement Learning Approach for Energy Management in Low Earth Orbit Satellite Electrical Power Systems
by Silvio Baccari, Elisa Mostacciuolo, Massimo Tipaldi and Valerio Mariani
Electronics 2025, 14(15), 3110; https://doi.org/10.3390/electronics14153110 - 5 Aug 2025
Abstract
Effective energy management in Low Earth Orbit satellites is critical, as inefficient energy management can significantly affect mission objectives. The dynamic and harsh space environment further complicates the development of effective energy management strategies. To address these challenges, we propose a Deep Reinforcement [...] Read more.
Effective energy management in Low Earth Orbit satellites is critical, as inefficient energy management can significantly affect mission objectives. The dynamic and harsh space environment further complicates the development of effective energy management strategies. To address these challenges, we propose a Deep Reinforcement Learning approach using Deep-Q Network to develop an adaptive energy management framework for Low Earth Orbit satellites. Compared to traditional techniques, the proposed solution autonomously learns from environmental interaction, offering robustness to uncertainty and online adaptability. It adjusts to changing conditions without manual retraining, making it well-suited for handling modeling uncertainties and non-stationary dynamics typical of space operations. Training is conducted using a realistic satellite electric power system model with accurate component parameters and single-orbit power profiles derived from real space missions. Numerical simulations validate the controller performance across diverse scenarios, including multi-orbit settings, demonstrating superior adaptability and efficiency compared to conventional Maximum Power Point Tracking methods. Full article
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18 pages, 1102 KiB  
Review
Exploring Human Sperm Metabolism and Male Infertility: A Systematic Review of Genomics, Proteomics, Metabolomics, and Imaging Techniques
by Achraf Zakaria, Idrissa Diawara, Amal Bouziyane and Noureddine Louanjli
Int. J. Mol. Sci. 2025, 26(15), 7544; https://doi.org/10.3390/ijms26157544 - 5 Aug 2025
Abstract
Male infertility is a multifactorial condition often associated with disruptions in sperm metabolism and mitochondrial function, yet traditional semen analysis provides limited insight into these molecular mechanisms. Understanding sperm bioenergetics and metabolic dysfunctions is crucial for improving the diagnosis and treatment of conditions [...] Read more.
Male infertility is a multifactorial condition often associated with disruptions in sperm metabolism and mitochondrial function, yet traditional semen analysis provides limited insight into these molecular mechanisms. Understanding sperm bioenergetics and metabolic dysfunctions is crucial for improving the diagnosis and treatment of conditions such as asthenozoospermia and azoospermia. This systematic review synthesizes recent literature, focusing on advanced tools and techniques—including omics technologies, advanced imaging, spectroscopy, and functional assays—that enable comprehensive molecular assessment of sperm metabolism and development. The reviewed studies highlight the effectiveness of metabolomics, proteomics, and transcriptomics in identifying metabolic biomarkers linked to male infertility. Non-invasive imaging modalities such as Raman and magnetic resonance spectroscopy offer real-time metabolic profiling, while the seminal microbiome is increasingly recognized for its role in modulating sperm metabolic health. Despite these advances, challenges remain in clinical validation and implementation of these techniques in routine infertility diagnostics. Integrating molecular metabolic assessments with conventional semen analysis promises enhanced diagnostic precision and personalized therapeutic approaches, ultimately improving reproductive outcomes. Continued research is needed to standardize biomarkers and validate clinical utility. Furthermore, these metabolic tools hold significant potential to elucidate the underlying causes of previously misunderstood and unexplained infertility cases, offering new avenues for diagnosis and treatment. Full article
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18 pages, 1814 KiB  
Article
Student’s t Kernel-Based Maximum Correntropy Criterion Extended Kalman Filter for GPS Navigation
by Dah-Jing Jwo, Yi Chang, Yun-Han Hsu and Amita Biswal
Appl. Sci. 2025, 15(15), 8645; https://doi.org/10.3390/app15158645 (registering DOI) - 5 Aug 2025
Abstract
Global Navigation Satellite System (GNSS) receivers may produce measurement outliers in real-world applications owing to various circumstances, including poor signal quality, multipath effects, data loss, satellite signal loss, or electromagnetic interference. This can lead to a noise distribution that is non-Gaussian heavy-tailed, affecting [...] Read more.
Global Navigation Satellite System (GNSS) receivers may produce measurement outliers in real-world applications owing to various circumstances, including poor signal quality, multipath effects, data loss, satellite signal loss, or electromagnetic interference. This can lead to a noise distribution that is non-Gaussian heavy-tailed, affecting the effectiveness of satellite navigation filters. This paper presents a robust Extended Kalman Filter (EKF) based on the Maximum Correntropy Criterion with a Student’s t kernel (STMCCEKF) for GPS navigation under non-Gaussian noise. Unlike traditional EKF and Gaussian-kernel MCCEKF, the proposed method enhances robustness by leveraging the heavy-tailed Student’s t kernel, which effectively suppresses outliers and dynamic observation noise. A fixed-point iterative algorithm is used for state update, and a new posterior error covariance expression is derived. The simulation results demonstrate that STMCCEKF outperforms conventional filters in positioning accuracy and robustness, particularly in environments with impulsive noise and multipath interference. The Student’s t-distribution kernel efficiently mitigates heavy-tailed non-Gaussian noise, while it adaptively adjusts process and measurement noise covariances, leading to improved estimation performance. A detailed explanation of several key concepts along with practical examples are discussed to aid in understanding and applying the Global Positioning System (GPS) navigation filter. By integrating cutting-edge reinforcement learning with robust statistical approaches, this work advances adaptive signal processing and estimation, offering a significant contribution to the field. Full article
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25 pages, 1751 KiB  
Review
Large Language Models for Adverse Drug Events: A Clinical Perspective
by Md Muntasir Zitu, Dwight Owen, Ashish Manne, Ping Wei and Lang Li
J. Clin. Med. 2025, 14(15), 5490; https://doi.org/10.3390/jcm14155490 - 4 Aug 2025
Abstract
Adverse drug events (ADEs) significantly impact patient safety and health outcomes. Manual ADE detection from clinical narratives is time-consuming, labor-intensive, and costly. Recent advancements in large language models (LLMs), including transformer-based architectures such as Bidirectional Encoder Representations from Transformers (BERT) and Generative Pretrained [...] Read more.
Adverse drug events (ADEs) significantly impact patient safety and health outcomes. Manual ADE detection from clinical narratives is time-consuming, labor-intensive, and costly. Recent advancements in large language models (LLMs), including transformer-based architectures such as Bidirectional Encoder Representations from Transformers (BERT) and Generative Pretrained Transformer (GPT) series, offer promising methods for automating ADE extraction from clinical data. These models have been applied to various aspects of pharmacovigilance and clinical decision support, demonstrating potential in extracting ADE-related information from real-world clinical data. Additionally, chatbot-assisted systems have been explored as tools in clinical management, aiding in medication adherence, patient engagement, and symptom monitoring. This narrative review synthesizes the current state of LLMs in ADE detection from a clinical perspective, organizing studies into categories such as human-facing decision support tools, immune-related ADE detection, cancer-related and non-cancer-related ADE surveillance, and personalized decision support systems. In total, 39 articles were included in this review. Across domains, LLM-driven methods have demonstrated promising performances, often outperforming traditional approaches. However, critical limitations persist, such as domain-specific variability in model performance, interpretability challenges, data quality and privacy concerns, and infrastructure requirements. By addressing these challenges, LLM-based ADE detection could enhance pharmacovigilance practices, improve patient safety outcomes, and optimize clinical workflows. Full article
(This article belongs to the Section Pharmacology)
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22 pages, 3270 KiB  
Article
Deep Point Cloud Facet Segmentation and Applications in Downsampling and Crop Organ Extraction
by Yixuan Wang, Chuang Huang and Dawei Li
Appl. Sci. 2025, 15(15), 8638; https://doi.org/10.3390/app15158638 (registering DOI) - 4 Aug 2025
Abstract
To address the issues in existing 3D point cloud facet generation networks, specifically, the tendency to produce a large number of empty facets and the uncertainty in facet count, this paper proposes a novel deep learning framework for robust facet segmentation. Based on [...] Read more.
To address the issues in existing 3D point cloud facet generation networks, specifically, the tendency to produce a large number of empty facets and the uncertainty in facet count, this paper proposes a novel deep learning framework for robust facet segmentation. Based on the generated facet set, two exploratory applications are further developed. First, to overcome the bottleneck where inaccurate empty-facet detection impairs the downsampling performance, a facet-abstracted downsampling method is introduced. By using a learned facet classifier to filter out and discard empty facets, retaining only non-empty surface facets, and fusing point coordinates and local features within each facet, the method achieves significant compression of point cloud data while preserving essential geometric information. Second, to solve the insufficient precision in organ segmentation within crop point clouds, a facet growth-based segmentation algorithm is designed. The network first predicts the edge scores for the facets to determine the seed facets. The facets are then iteratively expanded according to adjacent-facet similarity until a complete organ region is enclosed, thereby enhancing the accuracy of segmentation across semantic boundaries. Finally, the proposed facet segmentation network is trained and validated using a synthetic dataset. Experiments show that, compared with traditional methods, the proposed approach significantly outperforms both downsampling accuracy and instance segmentation performance. In various crop scenarios, it demonstrates excellent geometric fidelity and semantic consistency, as well as strong generalization ability and practical application potential, providing new ideas for in-depth applications of facet-level features in 3D point cloud analysis. Full article
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31 pages, 2983 KiB  
Review
Sustainable Management of Willow Forest Landscapes: A Review of Ecosystem Functions and Conservation Strategies
by Florin Achim, Lucian Dinca, Danut Chira, Razvan Raducu, Alexandru Chirca and Gabriel Murariu
Land 2025, 14(8), 1593; https://doi.org/10.3390/land14081593 - 4 Aug 2025
Abstract
Willow stands (Salix spp.) are an essential part of riparian ecosystems, as they sustain biodiversity and provide bioenergy solutions. The present review synthesizes the global scientific literature about the management of willow stands. In order to achieve this goal, we used a [...] Read more.
Willow stands (Salix spp.) are an essential part of riparian ecosystems, as they sustain biodiversity and provide bioenergy solutions. The present review synthesizes the global scientific literature about the management of willow stands. In order to achieve this goal, we used a dual approach combining bibliometric analysis with traditional literature review. As such, we consulted 416 publications published between 1978 and 2024. This allowed us to identify key species, ecosystem services, conservation strategies, and management issues. The results we have obtained show a diversity of approaches, with an increase in short-rotation coppice (SRC) systems and the multiple roles covered by willow stands (carbon sequestration, biomass production, riparian restoration, and habitat provision). The key trends we have identified show a shift toward topics such as climate resilience, ecological restoration, and precision forestry. This trend has become especially pronounced over the past decade (2014–2024), as reflected in the increasing use of these keywords in the literature. However, as willow systems expand in scale and function—from biomass production to ecological restoration—they also raise complex challenges, including invasive tendencies in non-native regions and uncertainties surrounding biodiversity impacts and soil carbon dynamics over the long term. The present review is a guide for forest policies and, more specifically, for future research, linking the need to integrate and use adaptive strategies in order to maintain the willow stands. Full article
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15 pages, 27119 KiB  
Article
Dehazing Algorithm Based on Joint Polarimetric Transmittance Estimation via Multi-Scale Segmentation and Fusion
by Zhen Wang, Zhenduo Zhang and Xueying Cao
Appl. Sci. 2025, 15(15), 8632; https://doi.org/10.3390/app15158632 (registering DOI) - 4 Aug 2025
Abstract
To address the significant degradation of image visibility and contrast in turbid media, this paper proposes an enhanced image dehazing algorithm. Unlike traditional polarimetric dehazing methods that exclusively attribute polarization information to airlight, our approach integrates object radiance polarization and airlight polarization for [...] Read more.
To address the significant degradation of image visibility and contrast in turbid media, this paper proposes an enhanced image dehazing algorithm. Unlike traditional polarimetric dehazing methods that exclusively attribute polarization information to airlight, our approach integrates object radiance polarization and airlight polarization for haze removal. First, sky regions are localized through multi-scale fusion of polarization and intensity segmentation maps. Second, region-specific transmittance estimation is performed by differentiating haze-occluded regions from haze-free regions. Finally, target radiance is solved using boundary constraints derived from non-haze regions. Compared with other dehazing algorithms, the method proposed in this paper demonstrates greater adaptability across diverse scenarios. It achieves higher-quality restoration of targets with results that more closely resemble natural appearances, avoiding noticeable distortion. Not only does it deliver excellent dehazing performance for land fog scenes, but it also effectively handles maritime fog environments. Full article
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17 pages, 5658 KiB  
Communication
When DNA Tells the Tale: High-Resolution Melting as a Forensic Tool for Mediterranean Cetacean Identification
by Mariangela Norcia, Alessia Illiano, Barbara Mussi, Fabio Di Nocera, Emanuele Esposito, Anna Di Cosmo, Domenico Fulgione and Valeria Maselli
Int. J. Mol. Sci. 2025, 26(15), 7517; https://doi.org/10.3390/ijms26157517 - 4 Aug 2025
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Abstract
Effective species identification is crucial for the conservation and management of marine mammals, particularly in regions such as the Mediterranean Sea, where several cetacean populations are endangered or vulnerable. In this study, we developed and validated a High-Resolution Melting (HRM) analysis protocol for [...] Read more.
Effective species identification is crucial for the conservation and management of marine mammals, particularly in regions such as the Mediterranean Sea, where several cetacean populations are endangered or vulnerable. In this study, we developed and validated a High-Resolution Melting (HRM) analysis protocol for the rapid, cost-effective, and reliable identification of the four representative marine cetacean species that occur in the Mediterranean Sea: the bottlenose dolphin (Tursiops truncatus), the striped dolphin (Stenella coeruleoalba), the sperm whale (Physeter macrocephalus), and the fin whale (Balaenoptera physalus). Species-specific primers targeting mitochondrial DNA regions (cytochrome b and D-loop) were designed to generate distinct melting profiles. The protocol was tested on both tissue and fecal samples, demonstrating high sensitivity, reproducibility, and discrimination power. The results confirmed the robustness of the method, with melting curve profiles clearly distinguishing the target species and achieving a success rate > 95% in identifying unknown samples. The use of HRM offers several advantages over traditional sequencing methods, including reduced cost, speed, portability, and suitability for degraded samples, such as those from the stranded individuals. This approach provides a valuable tool for non-invasive genetic surveys and real-time species monitoring, contributing to more effective conservation strategies for cetaceans and enforcement of regulations against illegal trade. Full article
(This article belongs to the Special Issue Molecular Insights into Zoology)
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44 pages, 6212 KiB  
Article
A Hybrid Deep Reinforcement Learning Architecture for Optimizing Concrete Mix Design Through Precision Strength Prediction
by Ali Mirzaei and Amir Aghsami
Math. Comput. Appl. 2025, 30(4), 83; https://doi.org/10.3390/mca30040083 - 3 Aug 2025
Viewed by 182
Abstract
Concrete mix design plays a pivotal role in ensuring the mechanical performance, durability, and sustainability of construction projects. However, the nonlinear interactions among the mix components challenge traditional approaches in predicting compressive strength and optimizing proportions. This study presents a two-stage hybrid framework [...] Read more.
Concrete mix design plays a pivotal role in ensuring the mechanical performance, durability, and sustainability of construction projects. However, the nonlinear interactions among the mix components challenge traditional approaches in predicting compressive strength and optimizing proportions. This study presents a two-stage hybrid framework that integrates deep learning with reinforcement learning to overcome these limitations. First, a Convolutional Neural Network–Long Short-Term Memory (CNN–LSTM) model was developed to capture spatial–temporal patterns from a dataset of 1030 historical concrete samples. The extracted features were enhanced using an eXtreme Gradient Boosting (XGBoost) meta-model to improve generalizability and noise resistance. Then, a Dueling Double Deep Q-Network (Dueling DDQN) agent was used to iteratively identify optimal mix ratios that maximize the predicted compressive strength. The proposed framework outperformed ten benchmark models, achieving an MAE of 2.97, RMSE of 4.08, and R2 of 0.94. Feature attribution methods—including SHapley Additive exPlanations (SHAP), Elasticity-Based Feature Importance (EFI), and Permutation Feature Importance (PFI)—highlighted the dominant influence of cement content and curing age, as well as revealing non-intuitive effects such as the compensatory role of superplasticizers in low-water mixtures. These findings demonstrate the potential of the proposed approach to support intelligent concrete mix design and real-time optimization in smart construction environments. Full article
(This article belongs to the Section Engineering)
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