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17 pages, 7833 KiB  
Article
Two-Year Post-Fire Abundance of Arthropod Groups Across Different Types of Forest in Temperate Central Europe
by Václav Zumr, Oto Nakládal and Jiří Remeš
Fire 2025, 8(8), 305; https://doi.org/10.3390/fire8080305 - 2 Aug 2025
Viewed by 314
Abstract
Forest fires are commonly regarded as negative for ecosystems; however, they also represent a major ecological force shaping the biodiversity of invertebrates and many other organisms. The aim of this study was to better understand how multiple groups of invertebrates respond to wildfire [...] Read more.
Forest fires are commonly regarded as negative for ecosystems; however, they also represent a major ecological force shaping the biodiversity of invertebrates and many other organisms. The aim of this study was to better understand how multiple groups of invertebrates respond to wildfire across different forest types in Central Europe. The research was conducted following a large forest fire (ca. 1200 ha) that occurred in 2022. Data were collected over two years (2023 and 2024), from April to September. The research was conducted in coniferous forests and included six pairwise study types: burnt and unburnt dead spruce (bark beetle affected), burnt and unburnt clear-cuts, and burnt and unburnt healthy stands. In total, 96 traps were deployed each year. Across both years, 220,348 invertebrates were recorded (1.Y: 128,323; 2.Y: 92,025), representing 24 taxonomic groups. A general negative trend in abundance following forest fire was observed in the groups Acari, Auchenorhyncha, Blattodea, Dermaptera, Formicidae, Chilopoda, Isopoda, Opiliones, and Pseudoscorionida. Groups showing a neutral response included Araneae, Coleoptera, Collembola, Diplopoda, Heteroptera, Psocoptera, Raphidioptera, Thysanoptera, and Trichoptera. Positive responses, indicated by an increase in abundance, were recorded in Hymenoptera, Orthoptera, Lepidoptera, and Diptera. However, considerable differences among management types (clear-cut, dead spruce, and healthy) were evident, as their distinct characteristics largely influenced invertebrate abundance in both unburnt and burnt variants of the types across all groups studied. Forest fire primarily creates favorable conditions for heliophilous, open-landscape, and floricolous invertebrate groups, while less mobile epigeic groups are strongly negatively affected. In the second year post-fire, the total invertebrate abundance in burnt sites decreased to 59% of the first year’s levels. Conclusion: Forest fire generates a highly heterogeneous landscape from a regional perspective, creating unique ecological niches that persist more than two years after fire. For many invertebrates, successional return toward pre-fire conditions is delayed or incomplete. Full article
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15 pages, 428 KiB  
Article
Biodiversity Patterns and Community Construction in Subtropical Forests Driven by Species Phylogenetic Environments
by Pengcheng Liu, Jiejie Jiao, Chuping Wu, Weizhong Shao, Xuesong Liu and Liangjin Yao
Plants 2025, 14(15), 2397; https://doi.org/10.3390/plants14152397 - 2 Aug 2025
Viewed by 487
Abstract
To explore the characteristics of species diversity and phylogenetic diversity, as well as the dominant processes of community construction, in different forest types (deciduous broad-leaved forest, mixed coniferous and broad-leaved forest, and Chinese fir plantation) in subtropical regions, analyze the specific driving patterns [...] Read more.
To explore the characteristics of species diversity and phylogenetic diversity, as well as the dominant processes of community construction, in different forest types (deciduous broad-leaved forest, mixed coniferous and broad-leaved forest, and Chinese fir plantation) in subtropical regions, analyze the specific driving patterns of soil nutrients and other environmental factors on the formation of forest diversity in different forest types, and clarify the differences in response to environmental heterogeneity between natural forests and plantation forests. Based on 48 fixed monitoring plots of 50 m × 50 m in Shouchang Forest Farm, Jiande City, Zhejiang Province, woody plants with a diameter at breast height ≥5 cm were investigated. Species diversity indices (Margalef index, Shannon–Wiener index, Simpson index, and Pielou index), phylogenetic structure index (PD), and environmental factors were used to analyze the relationship between diversity characteristics and environmental factors through variance analysis, correlation analysis, and generalized linear models. Phylogenetic structural indices (NRI and NTI) were used, combined with a random zero model, to explore the mechanisms of community construction in different forest types. Research has found that (1) the deciduous broad-leaved forest had the highest species diversity (Margalef index of 4.121 ± 1.425) and phylogenetic diversity (PD index of 21.265 ± 7.796), significantly higher than the mixed coniferous and broad-leaved forest and the Chinese fir plantation (p < 0.05); (2) there is a significant positive correlation between species richness and phylogenetic diversity, with the best fit being AIC = 70.5636 and R2 = 0.9419 in broad-leaved forests; however, the contribution of evenness is limited; (3) the specific effects of soil factors on different forest types: available phosphorus (AP) is negatively correlated with the diversity of deciduous broad-leaved forests (p < 0.05), total phosphorus (TP) promotes the diversity of coniferous and broad-leaved mixed forests, while the diversity of Chinese fir plantations is significantly negatively correlated with total nitrogen (TN); (4) the phylogenetic structure of three different forest types shows a divergent pattern in deciduous broad-leaved forests, indicating that competition and exclusion dominate the construction of deciduous broad-leaved forests; the aggregation mode of Chinese fir plantation indicates that environmental filtering dominates the construction of Chinese fir plantation; the mixed coniferous and broad-leaved forest is a transitional model, indicating that the mixed coniferous and broad-leaved forest is influenced by both stochastic processes and ecological niche processes. In different forest types in subtropical regions, the species and phylogenetic diversity of broad-leaved forests is significantly higher than in other forest types. The impact of soil nutrients on the diversity of different forest types varies, and the characteristics of community construction in different forest types are also different. This indicates the importance of protecting the original vegetation and provides a scientific basis for improving the ecological function of artificial forest ecosystems through structural adjustment. The research results have important practical guidance value for sustainable forest management and biodiversity conservation in the region. Full article
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20 pages, 718 KiB  
Communication
Examining Crisis Communication in Geopolitical Conflicts: The Micro-Influencer Impact Model
by Ahmed Taher, Hoda El Kolaly and Nourhan Tarek
Journal. Media 2025, 6(3), 116; https://doi.org/10.3390/journalmedia6030116 - 24 Jul 2025
Viewed by 399
Abstract
In the digital communication ecosystem, micro-influencers have influenced public response during crises, especially in complex geopolitical contexts. This paper introduces the micro-influencer impact model (MIIM), a framework for analyzing the impact of micro-influencers on crisis communication. The MIIM integrates four components (micro-influencer characteristics, [...] Read more.
In the digital communication ecosystem, micro-influencers have influenced public response during crises, especially in complex geopolitical contexts. This paper introduces the micro-influencer impact model (MIIM), a framework for analyzing the impact of micro-influencers on crisis communication. The MIIM integrates four components (micro-influencer characteristics, message framing and delivery, audience factors, and crisis context) offering a comprehensive approach to understanding micro-influencer dynamics during crises. Cross-conflict analysis spanning Ukraine–Russia, Sudan–Ethiopia, Armenia–Azerbaijan, Myanmar, Syria, and India–Pakistan tensions demonstrates the MIIM’s broad applicability across diverse geopolitical crises, showing how factors like perceived authenticity, niche expertise, narrative personalization, and audience digital literacy consistently shape public opinion and crisis response. The MIIM synthesizes crisis communication theories, social influence models, and digital media research, providing a sophisticated framework for studying the dissemination of information and public engagement during crises. The paper proposes theoretically grounded propositions on the impact of micro-influencers, encompassing perceived authenticity, narrative framing, and influence over time, thereby laying the groundwork for future empirical research. Implications for communication scholars, crisis managers, policymakers, and social media platforms are discussed, emphasizing the MIIM’s relevance to theory and practice in crisis communication. Full article
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21 pages, 9479 KiB  
Review
Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily
by Jean-Stéphane Venisse, Gisèle Bronner, Mouadh Saadaoui, Patricia Roeckel-Drevet, Mohamed Faize and Boris Fumanal
J. Fungi 2025, 11(7), 543; https://doi.org/10.3390/jof11070543 - 21 Jul 2025
Viewed by 349
Abstract
The fungal kingdom, with an estimated five million species, has undergone extensive diversification over the past billion years and now occupies a wide array of ecological niches from terrestrial to aquatic ecosystems. To thrive in such diverse environments, fungi must exhibit finely tuned [...] Read more.
The fungal kingdom, with an estimated five million species, has undergone extensive diversification over the past billion years and now occupies a wide array of ecological niches from terrestrial to aquatic ecosystems. To thrive in such diverse environments, fungi must exhibit finely tuned physiological and morphological responses orchestrated by conserved molecular pathways. Increasing evidence suggests that aquaporins (AQPs) play a key role in mediating these adaptive responses, particularly under varying abiotic and biotic stress conditions. However, despite notable advances in recent decades, the precise functional roles of AQPs within the fungal kingdom remains largely unresolved in the field of cell biology. AQPs are transmembrane proteins belonging to the major intrinsic proteins (MIPs) superfamily, which is characterized by remarkable sequence and structural diversity. Beyond their established function in facilitating water transport, MIPs mediated the bidirectional diffusion of a range of small inorganic and organic solutes, ions, and gases across cellular membranes. In fungi, MIPs are classified into three main subfamilies: orthodox (i.e., classical) AQPs, aquaglyceroporins (AQGP), and X-intrinsic proteins (XIPs). This review provides a concise summary of the fundamental structural and functional characteristics of fungal aquaporins, including their structure, classification, and known physiological roles. While the majority of the current literature has focused on the aquaporin and aquaglyceroporin subfamilies, this review also aims to offer a comprehensive and original overview of the relatively understudied X-intrinsic protein subfamily, highlighting its potential implication in fungal biology. Full article
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26 pages, 1699 KiB  
Review
Tumor Niche Influences the Activity and Delivery of Anticancer Drugs: Pharmacology Meets Chemistry
by Mauro Ravera, Elisabetta Gabano, Stelvio Tonello and Donato Colangelo
Pharmaceuticals 2025, 18(7), 1047; https://doi.org/10.3390/ph18071047 - 17 Jul 2025
Viewed by 442
Abstract
Cellular and molecular characteristics of the tumor microenvironment are fundamental for the formation of niches. These structures include both cellular and matrix components and have been shown to protect and promote cancer formation and progression. The peculiarities of tumor niches have been suggested [...] Read more.
Cellular and molecular characteristics of the tumor microenvironment are fundamental for the formation of niches. These structures include both cellular and matrix components and have been shown to protect and promote cancer formation and progression. The peculiarities of tumor niches have been suggested by many authors as targets with high therapeutic potential. This narrative review analyzes the chemical characteristics of the tumor microenvironment and describes experimental and clinical approaches to influence its contribution to cancer promotion and the spread of metastases. In particular, the possible chemical differences, like pH, oxygen levels, and cell composition, to be used for the design of drugs or the delivery of antiproliferative moieties for a more precise oncology approach, will be discussed. The literature proposes a vast number of molecules, but this review focuses on hypoxia-activated molecules, pH-sensitive nanocarriers, metal-based drugs, and gasotransmitters targeting selectively the tumor microenvironment as possible negative modulators of the contribution of niches to tumor promotion. The chemical peculiarities of the tumor niche are discussed for possible pharmacological developments. Full article
(This article belongs to the Section Pharmacology)
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32 pages, 19967 KiB  
Article
A Comprehensive Study on Gravlax: A Multidimensional Evaluation of Gravlax Produced from Different Fish Species and Herbs
by Can Okan Altan
Foods 2025, 14(14), 2465; https://doi.org/10.3390/foods14142465 - 14 Jul 2025
Viewed by 379
Abstract
In this study, gravlax, a niche Scandinavian delicacy, was comprehensively investigated by producing it with combinations of two different fish species (tub gurnard (Chelidonichthys lucerna Linnaeus, 1758) and garfish (Belone belone Linnaeus, 1761)) and five herbs (dill (Anethum graveolens Linnaeus, [...] Read more.
In this study, gravlax, a niche Scandinavian delicacy, was comprehensively investigated by producing it with combinations of two different fish species (tub gurnard (Chelidonichthys lucerna Linnaeus, 1758) and garfish (Belone belone Linnaeus, 1761)) and five herbs (dill (Anethum graveolens Linnaeus, 1753), sage (Salvia officinalis Linnaeus, 1753), mint (Mentha piperita Linnaeus, 1753), sweet (Ocimum basilicum Linnaeus, 1754), and purple basil (Ocimum basilicum var. purpurascens Bentham, 1830)). The nutritional composition, amino acids, color parameters, mineral substances, and heavy metal content, as well as physical characteristics, texture profile analysis, and extensive sensory analyses, were conducted, and the results were thoroughly evaluated using multivariate statistical methods. The influence of using different herbs on nutritional composition was found to be significant in gravlax made from both fish species (p < 0.05). Sensory analyses revealed that panelists identified mint as enhancing aroma and umami sensations, while dill improved overall acceptance. Gravlax with sage exhibited softer textures, but lower general acceptance due to perceived high saltiness. Color analyses revealed that purple basil transferred distinct pigments, causing darkening, whereas sweet basil had a brightening effect. Amino acid analyses revealed higher umami and sweet-tasting amino acids in herb-free gravlax, whereas proteolytic activity appeared to slow down in herb-containing gravlax samples. Full article
(This article belongs to the Section Sensory and Consumer Sciences)
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21 pages, 10389 KiB  
Article
Functional Low-Fat Goat Feta Cheese Formulated with Dietary Fiber as a Fat Replacer: Physicochemical, Textural, and Sensory Interactions
by Malaiporn Wongkaew, Bow Tinpovong, Aekarin Inpramoon, Pikulthong Chaimongkol, Auengploy Chailangka, Sureerat Thomya and Nuttinee Salee
Dairy 2025, 6(4), 31; https://doi.org/10.3390/dairy6040031 - 28 Jun 2025
Viewed by 449
Abstract
Consumer scrutiny of fat content in foods is becoming a notable trend in health concerns. This study aims to develop a novel functional low-fat goat feta cheese by utilizing polydextrose (PDX) and inulin as dietary fiber-based fat replacers to improve its overall characteristics. [...] Read more.
Consumer scrutiny of fat content in foods is becoming a notable trend in health concerns. This study aims to develop a novel functional low-fat goat feta cheese by utilizing polydextrose (PDX) and inulin as dietary fiber-based fat replacers to improve its overall characteristics. The physicochemical and textural properties, along with consumer acceptance, of the feta cheese were evaluated across three fat levels (full-fat [FFC], reduced-fat [RFC], low-fat [LFC]) and three fibers: PDX, inulin, and their combination. The intercorrelation of all characteristics was assessed through principal component analysis and Pearson’s correlation. Fat reduction significantly altered the cheese’s visual properties, increasing lightness and the total color difference, which inversely correlated with a* and b* values. Lower-fat cheeses exhibited decreased pH and increased lactic acid, with salinity playing a crucial role in both lactic acid development and texture. Under Scanning Electron Microscopy (SEM), PDX yielded a cheese matrix with a finer pore structure than inulin or the combined fibers. Lower-fat cheeses exhibited greater hardness, with PDX resulting in the highest hardness among the fiber treatments. Crucially, the RFC with PDX was as well-received by consumers as the FFC. These findings not only empower goat farmers and cheese entrepreneurs to increase their product value for niche market but also contribute to sustainability by providing a healthier food option for functional benefits. Full article
(This article belongs to the Section Milk Processing)
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35 pages, 1374 KiB  
Article
Sustainable Development of Industry-Specific Universities in China Under the “Double First-Class” Initiative: A Niche Perspective
by Bin Dong, Yuchen Wang, Bo Chen, Ruijun Zhang and Shengzhong Zhang
Sustainability 2025, 17(13), 5736; https://doi.org/10.3390/su17135736 - 22 Jun 2025
Viewed by 724
Abstract
China has made a major strategic decision to build world-class universities and first-class disciplines (abbreviation: Double First-Class), aimed at enhancing the global competitiveness of Chinese higher education. Industry-specific universities have a special historical evolution and distinctive characteristics. China’s industry-specific universities have always played [...] Read more.
China has made a major strategic decision to build world-class universities and first-class disciplines (abbreviation: Double First-Class), aimed at enhancing the global competitiveness of Chinese higher education. Industry-specific universities have a special historical evolution and distinctive characteristics. China’s industry-specific universities have always played an important role in the higher education system and made significant contributions to the development of the country. However, the “Double First-Class” initiative presents both opportunities and challenges for industry-specific universities. This paper employs the SWOT analysis method to conduct a qualitative analysis of industry-specific universities and proposes a strategic matrix for decision-making. At the same time, from a niche perspective, this paper explores the sustainable development strategies of these institutions within the initiative through the calculation of niche breadth, niche overlap, and their relationship analysis. The research results indicate that the “Double First-Class” initiative has played a positive role in promoting the expansion of universities’ ecological niches. However, it has also led to excessive niche overlap and intense competition. Industry-specific universities face opportunities and challenges in terms of structure, strategy, and policy for their sustainable development. Key findings highlight the importance of strategic alignment with national demand, industry cooperation, and policy orientation for sustainable growth. This paper proposes recommendations for the construction of a sustainable development framework, implementation of strategic initiatives, and policy guidance for universities with industrial characteristics from three perspectives: government, industry, and universities. Full article
(This article belongs to the Section Sustainable Education and Approaches)
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12 pages, 425 KiB  
Perspective
Exploring Use-Rates of and Scientific Evidence on Sutureless Devices in Aortic Valve Replacement: A Bibliographic Meta-Analysis and Clinical Considerations
by Cristiano Spadaccio, Arnaldo Dimagli, Clayton J. Agler, Dane C. Paneitz, Stanley B. Wolfe, Antonio Nenna, Asishana A. Osho and David Rose
J. Clin. Med. 2025, 14(12), 4049; https://doi.org/10.3390/jcm14124049 - 7 Jun 2025
Viewed by 535
Abstract
Sutureless aortic valve replacement (SuAVR) has emerged as a potential alternative to conventional surgical aortic valve replacement (SAVR), particularly in minimally invasive settings. However, its global adoption remains limited, with a notable concentration of use and scientific production in select European countries. This [...] Read more.
Sutureless aortic valve replacement (SuAVR) has emerged as a potential alternative to conventional surgical aortic valve replacement (SAVR), particularly in minimally invasive settings. However, its global adoption remains limited, with a notable concentration of use and scientific production in select European countries. This bibliographic meta-analysis systematically reviewed 538 studies to assess the evidence landscape surrounding SuAVR, highlighting a predominance of observational data, sparse randomized controlled trials (1.3%), and significant geographical imbalances in research output. Europe accounted for 80% of publications, while North America contributed less than 10%. Key structural factors—including reimbursement policies, earlier regulatory approvals, and population characteristics—appear to influence SuAVR adoption. Despite procedural advantages such as reduced cross-clamp times, concerns over cost, pacemaker implantation rates, and uncertain long-term durability persist. Importantly, SuAVR may offer its greatest clinical value by facilitating minimally invasive surgery, a niche still underutilized worldwide. The limited randomized data and industrial focus on transcatheter approaches have further hindered widespread acceptance. Our findings underscore the need for high-quality comparative trials and standardized guidelines to define the role of SuAVR in modern valve therapy. Full article
(This article belongs to the Section Cardiology)
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15 pages, 1619 KiB  
Article
Characterization of Aroma, Sensory Properties, and Consumer Acceptability of Honey from Capparis spinosa L.
by Gianluca Tripodi, Maria Merlino, Marco Torre, Concetta Condurso, Antonella Verzera and Fabrizio Cincotta
Foods 2025, 14(11), 1978; https://doi.org/10.3390/foods14111978 - 3 Jun 2025
Viewed by 571
Abstract
The increasing scarcity of traditional nectar sources due to climate change has led beekeepers to explore alternative floral sources. This study investigates the volatile profile, sensory characteristics, and consumer acceptability of monofloral honey derived from Capparis spinosa L., a drought-resistant Mediterranean plant. Honey [...] Read more.
The increasing scarcity of traditional nectar sources due to climate change has led beekeepers to explore alternative floral sources. This study investigates the volatile profile, sensory characteristics, and consumer acceptability of monofloral honey derived from Capparis spinosa L., a drought-resistant Mediterranean plant. Honey samples produced by Apis mellifera ssp. sicula on Aeolian Islands (Sicily, Italy) were analyzed. Volatile organic compounds (VOCs) were extracted using headspace solid–phase microextraction (HS-SPME) and identified by gas chromatography–mass spectrometry (GC–MS), revealing 59 compounds, with dimethyl sulfide being the predominant one. Sensory evaluation using quantitative descriptive analysis (QDA) and Time Intensity (TI) analysis identified distinctive descriptors such as sweet-caramel, cabbage/cauliflower, and pungent notes. Statistical analyses confirmed correlations between specific VOCs and sensory perceptions. A consumer acceptability test involving 80 participants showed lower preference scores for caper honey in terms of aroma and overall acceptability compared to commercial multifloral honey, with differences observed across age groups. The unique aromatic profile and consumer feedback suggest that caper honey has strong potential as a niche, high-quality product, particularly within the context of climate-resilient beekeeping, offering valuable opportunities for innovation and diversification in sustainable apiculture. Full article
(This article belongs to the Special Issue Novel Insights into Food Flavor Chemistry and Analysis)
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18 pages, 2888 KiB  
Article
Effects of Stand Structure on Aboveground Biomass in Mixed Moso Bamboo Forests in Tianbaoyan National Nature Reserve, Fujian, China
by Ziyun Deng, Qing Xu, Shaohui Fan, Songpo Wei, Guanglu Liu, Zhiteng Li and Changtang Cai
Forests 2025, 16(6), 905; https://doi.org/10.3390/f16060905 - 28 May 2025
Viewed by 368
Abstract
Forest aboveground biomass (AGB) serves as a crucial indicator of productivity and carbon storage capacity. While the impact of stand structure on AGB is well-documented for pure moso bamboo stands, the specific structural factors influencing AGB and the mechanisms driving these effects in [...] Read more.
Forest aboveground biomass (AGB) serves as a crucial indicator of productivity and carbon storage capacity. While the impact of stand structure on AGB is well-documented for pure moso bamboo stands, the specific structural factors influencing AGB and the mechanisms driving these effects in mixed moso bamboo forests, characterized by species diversity and structural complexity, require further elucidation. This study analyzed 9453 bamboos and arbor trees within the TianBao MetaPlot, which were tessellated into 108 standard plots in Tianbaoyan National Nature Reserve, Fujian, China. Using a multi-method voting approach, we identified the key structural factors influencing stand AGB and employed Partial Least Squares Path Modeling (PLS-PM) to assess their direct and indirect effects. We found that the stand density, moso bamboo mixing ratio, Shannon’s index, Simpson’s index, mean tree height, openness, and tree size variation coefficient were the key structural factors influencing the stand AGB. The PLS-PM analysis showed that stand density had a negative effect on stand AGB, which can be explicitly decomposed through a direct negative effect and an indirect negative effect. Tree diversity showed a strong positive effect, supporting the niche complementarity theory. The stand mean tree height and stand tree size variation had positive effects on stand AGB, while stand openness had a negative effect. The direct effects of tree diversity, stand mean tree height, and stand openness were stronger than the indirect effects on stand AGB, while the indirect effect of stand density was greater than the aforementioned effects. These results highlight the complex interactions between stand structure and stand AGB in mixed moso bamboo forests. The negative effect of stand density on stand AGB is in contrast with previous findings on arbor forests, wherein a higher stand density often promotes AGB, highlighting the unique structural characteristics of mixed moso bamboo forests. To promote biomass accumulation and enhance carbon sequestration in mixed moso bamboo stands, it is recommended to increase the tree size variability, enhance the tree species diversity, and apply rational thinning of moso bamboo, based on site-specific conditions. Full article
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18 pages, 18559 KiB  
Article
Dynamic Restoration of Collapsed Anammox Biofilm Systems: Integrating Process Optimization, Microbial Community Succession, and Machine Learning-Based Prediction
by Li Wang, Yongxing Chen, Junfeng Yang, Jiayi Li, Yu Zhang and Xiaojun Wang
Processes 2025, 13(6), 1672; https://doi.org/10.3390/pr13061672 - 26 May 2025
Viewed by 464
Abstract
The majority of extant studies concentrate on the reactivation of dormant Anammox biomass or the recovery of activity under specific storage conditions. Research on rehabilitation strategies for anaerobic ammonium oxidation (Anammox) systems is limited, with the exception of research on inhibitory factors. The [...] Read more.
The majority of extant studies concentrate on the reactivation of dormant Anammox biomass or the recovery of activity under specific storage conditions. Research on rehabilitation strategies for anaerobic ammonium oxidation (Anammox) systems is limited, with the exception of research on inhibitory factors. The recovery characteristics of biofilm systems after collapse induced by varying degrees of ammonia-nitrogen and small-molecular organic compound composite shocks have not been thoroughly elucidated. This study addresses the collapse of Anammox biofilm systems caused by sodium acetate inhibition through multi-phase rehabilitation strategies, stoichiometric analysis, and microbial community succession dynamics. Two regression algorithms—Support Vector Regression (SVR) and eXtreme Gradient Boosting (XGBoost)—were employed to construct predictive models for Total Nitrogen Removal Efficiency (TNRE) and Total Nitrogen Removal Rate (TNRR) in the CANON system, with model performance evaluated via coefficient of determination (R2) and root mean square error (RMSE). Results demonstrated that after terminating moderate-to-high sodium acetate dosing (300 mg/L and 500 mg/L), reactors R300 and R500 achieved TNRE recovery to 57.98% and 58.86%, respectively, and TNRR of 0.281 and 0.275 kgN/m3·d within 60–100 days, indicating the reversibility of high-concentration sodium acetate inhibition but a positive correlation between recovery duration and inhibition intensity. Microbial community analysis revealed that Planctomycetota (including Candidatus_Kuenenia) rebounded to 46–49% relative abundance in R100, synchronized with TNRE improvement. In contrast, R300 and R500 exhibited ecological niche replacement of denitrifiers (Denitratisoma) and partial TNRE restoration despite enhanced performance. Model comparisons showed SVR outperformed XGBoost in TNRE prediction, whereas XGBoost demonstrated superior TNRR prediction accuracy with R2 approaching 1 and RMSE nearing 0, significantly surpassing SVR. This work provides critical insights into recovery mechanisms under organic inhibition stress and establishes a robust predictive framework for optimizing nitrogen removal performance in CANON systems. Full article
(This article belongs to the Special Issue Applications of Microorganisms in Wastewater Treatment Processes)
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21 pages, 4432 KiB  
Article
Soil Fungal Diversity, Community Structure, and Network Stability in the Southwestern Tibetan Plateau
by Shiqi Zhang, Zhenjiao Cao, Siyi Liu, Zhipeng Hao, Xin Zhang, Guoxin Sun, Yuan Ge, Limei Zhang and Baodong Chen
J. Fungi 2025, 11(5), 389; https://doi.org/10.3390/jof11050389 - 19 May 2025
Viewed by 754
Abstract
Despite substantial research on how environmental factors affect fungal diversity, the mechanisms shaping regional-scale diversity patterns remain poorly understood. This study employed ITS high-throughput sequencing to evaluate soil fungal diversity, community composition, and co-occurrence networks across alpine meadows, desert steppes, and alpine shrublands [...] Read more.
Despite substantial research on how environmental factors affect fungal diversity, the mechanisms shaping regional-scale diversity patterns remain poorly understood. This study employed ITS high-throughput sequencing to evaluate soil fungal diversity, community composition, and co-occurrence networks across alpine meadows, desert steppes, and alpine shrublands in the southwestern Tibetan Plateau. We found significantly higher fungal α-diversity in alpine meadows and desert steppes than in alpine shrublands. Random forest and CAP analyses identified the mean annual temperature (MAT) and normalized difference vegetation index (NDVI) as major ecological drivers. Mantel tests revealed that soil physicochemical properties explained more variation than climate, indicating an indirect climatic influence via soil characteristics. Distance–decay relationships suggested that environmental heterogeneity and species interactions drive community isolation. Structural equation modeling confirmed that the MAT and NDVI regulate soil pH and carbon/nitrogen availability, thereby influencing fungal richness. The highly modular fungal co-occurrence network depended on key nodes for connectivity. Vegetation coverage correlated positively with network structure, while soil pH strongly affected network stability. Spatial heterogeneity constrained stability and diversity through resource distribution and niche segregation, whereas stable networks concentrated resources among dominant species. These findings enhance our understanding of fungal assemblage processes at a regional scale, providing a scientific basis for the management of soil fungal resources in plateau ecosystems. Full article
(This article belongs to the Section Environmental and Ecological Interactions of Fungi)
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21 pages, 4707 KiB  
Article
The Impact of Litter from Different Belowground Organs of Phragmites australis on Microbial-Mediated Soil Organic Carbon Accumulation in a Lacustrine Wetland
by Chong Chen, Yong Wang, Liu Yang, Yongen Min, Keming Yue, Sitong Lu, Hongfeng Bian, Xue Wang and Leilei Zhang
Microorganisms 2025, 13(5), 1146; https://doi.org/10.3390/microorganisms13051146 - 16 May 2025
Viewed by 439
Abstract
Although belowground litter decomposition critically influences lacustrine wetland soil carbon dynamics, the organ-specific microbial mechanisms driving soil organic carbon (SOC) accumulation remain unclear. Existing research has predominantly focused on aboveground litter, leaving a significant gap in the understanding of how roots and rhizomes [...] Read more.
Although belowground litter decomposition critically influences lacustrine wetland soil carbon dynamics, the organ-specific microbial mechanisms driving soil organic carbon (SOC) accumulation remain unclear. Existing research has predominantly focused on aboveground litter, leaving a significant gap in the understanding of how roots and rhizomes differentially regulate carbon cycling through microbial community assembly and survival strategies. This study took Phragmites australis (a plant characteristic of lacustrine wetland) as the research object and examined how decomposing belowground litter from different organs affects microbial-mediated SOC accumulation through a one-year in situ field incubation in Jingyuetan National Forest Park, Changchun City, Jilin Province, China. Our findings reveal that root litter exhibited the highest decomposition rate, which was accelerated by intermittent flooding, reaching up to 1.86 times that of rhizome. This process enriched r-strategist microbial taxa, intensified homogeneous selection, and expanded niche width, directly promoting SOC accumulation. Rhizome litter decomposition enhanced dispersal limitation, promoted K-strategist microbial dominance, and indirectly modulated SOC through soil acidification. Mixed-litter treatments significantly enhanced SOC accumulation (up to three times higher than single-litter treatments) through synergistic nutrient release (non-additive effects < 0.04) and reinforced microbial network interactions. SOC accumulation varied significantly with the flooding regime as follows: non-flooded > intermittent flooding > permanent flooding. This study provides new insights into the microbially driven mechanisms of plant-organ-specific decomposition in the carbon cycling of wetland ecosystems. Full article
(This article belongs to the Section Environmental Microbiology)
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31 pages, 105996 KiB  
Article
Archaeological Analysis of the Newly Discovered Tomb with a Relief of a Couple at the Funerary Area of Porta Sarno in Pompeii
by Llorenç Alapont, Rachele Cava, Joaquin Alfonso Llorens, Juan José Ruiz Lopez, Ana Miguélez González, Pilar Mas Hurtuna, Tomas Hurtado Mullor, Victor Revilla, Antoni Puig Palerm, Silvia Alfayé Vila, Altea Gadea Matamoros, Esther Alba Pagan and Sophie Hay
Heritage 2025, 8(5), 174; https://doi.org/10.3390/heritage8050174 - 16 May 2025
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Abstract
In July 2024, the “Investigating the Archaeology of Death in Pompeii Research Project” carried out a scientific and methodical excavation of the areas outside two of the gates to the city of Pompeii. One of them is the funerary area of Porta Nola [...] Read more.
In July 2024, the “Investigating the Archaeology of Death in Pompeii Research Project” carried out a scientific and methodical excavation of the areas outside two of the gates to the city of Pompeii. One of them is the funerary area of Porta Nola (next to the tomb of Obellio Firmo) and the other is outside Porta Sarno area (east of the tomb of Marcus Venerius Secundius). The investigated funerary area to the east of Porta Sarno corresponds with the area excavated in 1998 for the construction of the double Circumvesuviana rails. The 1998 excavations recorded the presence of more than 50 cremation burial sites, marked by stelae (columelle) and a monument with an arch, which are delineated by a boundary wall. The tombs were initially dated to the Late Republican period. In order to carry out comprehensive studies of the funerary area uncovered in 1998, a four metre by four metre trench was stratigraphically excavated. This investigation allowed mapping of the area and the carrying out of archaeological analysis and bioarchaeological studies in order to answer the questions that guided our archaeological research, such as whether the funerary area was abandoned and, if so, when? What was the chronological succession, monumentality, and prestige of this funerary space? Was it a single family and private funerary enclosure, or was it an open public space? How were this funerary area and the spaces destined to preserve the memory of the deceased managed? How were the funerary and mortuary rituals and gestures articulated and what did they consist of? Our methodical excavation discovered a monumental tomb which allows us to answer many of the questions raised by our research. This extraordinary monument consists of a wide wall with several niches containing the cremated remains of the deceased built into its structure and which is crowned by a relief of a young couple. The symbolism of the carved accessories of the wife may identify her as a priestess of Ceres. Additionally, the quality of the carving in the sculptures and their archaic characteristics suggest a Republic period dating, which is uncommon in southern Italy. Full article
(This article belongs to the Special Issue Advances in Archaeology and Anthropology of the Ancient World)
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