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31 pages, 340 KiB  
Article
Goal Setting for Teacher Development: Enhancing Culturally Responsive, Inclusive, and Social Justice Pedagogy
by Lydiah Nganga, Aaron Nydam and John Kambutu
Educ. Sci. 2025, 15(3), 264; https://doi.org/10.3390/educsci15030264 - 20 Feb 2025
Cited by 1 | Viewed by 2288
Abstract
This interpretive phenomenological study explores the perspectives of in-service, K-12 teachers in a graduate teacher education program on using goal setting to promote culturally responsive practices. The participants set two goals, documented their growth, and rated course instructional strategies that contributed to their [...] Read more.
This interpretive phenomenological study explores the perspectives of in-service, K-12 teachers in a graduate teacher education program on using goal setting to promote culturally responsive practices. The participants set two goals, documented their growth, and rated course instructional strategies that contributed to their learning. Data were collected through written reflections and responses to an anonymous midterm questionnaire in an online forum. Additional data came from the participants’ final reflections, the principal researcher’s reflective teaching notes, and end-of-semester reflections gathered by an unaffiliated critical friend after grades were posted. Coded data revealed that: 1. In-service teachers created goals that were relevant to specific areas of growth in professional learning and development in culturally responsive teaching practices and understanding diverse learners, 2. Goal setting, as a self-regulated learning strategy, serves to empower teachers toward teaching for promoting culturally responsive teaching practices, 3. Instructional supports and activities enhance personal development in becoming culturally responsive teachers, 4. Teacher educators’ reflection-on-action supports professional development. Full article
18 pages, 262 KiB  
Article
Culturally Responsive Professional Development Programs for Teacher Educators Using Community-Based Collaborative Learning: Lessons Learned from a Native American Community
by Lydiah Nganga and John Kambutu
Educ. Sci. 2024, 14(7), 787; https://doi.org/10.3390/educsci14070787 - 19 Jul 2024
Cited by 5 | Viewed by 6071
Abstract
Earners from Native American communities in the U.S. experience unfamiliar curricula that are designed and implemented by educators from the dominant culture who are often unprepared to meet the needs of diverse leaners. Consequently, teacher education programs have a responsibility to equipe preservice [...] Read more.
Earners from Native American communities in the U.S. experience unfamiliar curricula that are designed and implemented by educators from the dominant culture who are often unprepared to meet the needs of diverse leaners. Consequently, teacher education programs have a responsibility to equipe preservice teachers with knowledge and skills essential to integrating culturally relevant contents. Therefore, this collaborative autoethnography conducted as a teacher-educator professional development program, explored the perspectives of Native Peoples on preferred pedagogical approaches for Indigenous learners. Data were collected through autobiographical notes, written reflections, focus groups, interactive activities with study participants, and structured interviews. A thematic analysis resulted in two findings, that is, importance of contextually appropriate curriculum and the value of community-based collaborative learning professional development activities in supporting authentic culturally responsive lessons for teacher educators. Among other recommendations, the data support the enactment of teacher education programs that support intentional culturally collaborative Community-Based Professional Development activities between educators and Indigenous Peoples. Full article
(This article belongs to the Special Issue Hybrid Ecologies for Teacher Professional Learning)
17 pages, 618 KiB  
Article
Concept Mapping STI/HIV Prevention and Condom Use among Young African American Adults
by Chakema Carmack, Sarah Nganga, Eisha Ahmed and Taylor Coleman
Behav. Sci. 2024, 14(6), 501; https://doi.org/10.3390/bs14060501 - 14 Jun 2024
Viewed by 1749
Abstract
Theory-based HIV prevention programs have resulted in increased condom use, which remains the best method for the prevention of sexually transmitted infections (STIs) among sexually active heterosexual individuals. Particularly, the integrative model of behavior prediction theorizes that attitudes, norms, self-efficacy, and socioenvironmental factors [...] Read more.
Theory-based HIV prevention programs have resulted in increased condom use, which remains the best method for the prevention of sexually transmitted infections (STIs) among sexually active heterosexual individuals. Particularly, the integrative model of behavior prediction theorizes that attitudes, norms, self-efficacy, and socioenvironmental factors influence intention and behavior and has been useful in understanding STI risk among adolescents. However, more research is needed regarding young African American adults. Given the increased freedom and decision-making independence afforded to young adults compared to adolescents, it is important to consider the STI/human immunodeficiency virus (HIV) prevention messages that would resonate with them, particularly regarding condom use. The present study sought to explore how attitudes, subjective norms, self-efficacies, and socioenvironmental factors may influence condom use and STI/HIV prevention, as conceptualized by the participants. We conducted a group-based concept map, a systems-thinking mixed methodology that resulted in a geospatial map reflecting the conceptualizations of the participants. Self-identified young heterosexual African American adults (N = 43) aged 20–26 engaged in an interactive concept mapping procedure in order to “map out” their overarching concepts about STI/HIV risk and condom use. Seven overall conceptual domains emerged: self-efficacy for partner communication, condom use self-efficacy, social media/sociocultural influences, condom use/STI knowledge, condom use cons, condom use pros, and subjective and social norms about condom use. We presented the concept map and discussed the conceptual interpretations and the relationships among the overarching concepts. We also discussed how the social environment, including the social media environment, was conceptualized regarding STI/HIV risk and prevention among young African American adults. Concept mapping can be viewed as a way to determine worthwhile messages for intervention development. The findings may provide information for prevention programs aimed at reducing the incidence of STIs among young adult heterosexual persons within African American communities. Full article
(This article belongs to the Special Issue Psychosocial Factors and Risk Behavior among Minority Populations)
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17 pages, 683 KiB  
Article
Bridging the Gap: Enhancing Maritime Vessel Cyber Resilience through Security Operation Centers
by Allan Nganga, George Nganya, Margareta Lützhöft, Steven Mallam and Joel Scanlan
Sensors 2024, 24(1), 146; https://doi.org/10.3390/s24010146 - 27 Dec 2023
Cited by 8 | Viewed by 2899
Abstract
Increasingly disruptive cyber-attacks in the maritime domain have led to more efforts being focused on enhancing cyber resilience. From a regulatory perspective, there is a requirement that maritime stakeholders implement measures that would enable the timely detection of cyber events, leading to the [...] Read more.
Increasingly disruptive cyber-attacks in the maritime domain have led to more efforts being focused on enhancing cyber resilience. From a regulatory perspective, there is a requirement that maritime stakeholders implement measures that would enable the timely detection of cyber events, leading to the adoption of Maritime Security Operation Centers (M-SOCs). At the same time, Remote Operation Centers (ROCs) are also being discussed to enable increased adoption of highly automated and autonomous technologies, which could further impact the attack surface of vessels. The main objective of this research was therefore to better understand both enabling factors and challenges impacting the effectiveness of M-SOC operations. Semi-structured interviews were conducted with nine M-SOC experts. Informed by grounded theory, incident management emerged as the core category. By focusing on the factors that make M-SOC operations a unique undertaking, the main contribution of this study is that it highlights how maritime connectivity challenges and domain knowledge impact the M-SOC incident management process. Additionally, we have related the findings to a future where M-SOC and ROC operations could be converged. Full article
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15 pages, 2362 KiB  
Article
Association Mapping of Candidate Genes Associated with Iron and Zinc Content in Rice (Oryza sativa L.) Grains
by Chance Bahati Bukomarhe, Paul Kitenge Kimwemwe, Stephen Mwangi Githiri, Edward George Mamati, Wilson Kimani, Collins Mutai, Fredrick Nganga, Paul-Martin Dontsop Nguezet, Jacob Mignouna, René Mushizi Civava and Mamadou Fofana
Genes 2023, 14(9), 1815; https://doi.org/10.3390/genes14091815 - 18 Sep 2023
Cited by 5 | Viewed by 2791
Abstract
Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), in the diet contribute to health issues and hidden hunger. Enhancing the Fe and Zn content in globally staple food crops like rice is necessary to address food malnutrition. A Genome-Wide Association Study (GWAS) [...] Read more.
Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), in the diet contribute to health issues and hidden hunger. Enhancing the Fe and Zn content in globally staple food crops like rice is necessary to address food malnutrition. A Genome-Wide Association Study (GWAS) was conducted using 85 diverse rice accessions from the Democratic Republic of Congo (DRC) to identify genomic regions associated with grain Fe and Zn content. The Fe content ranged from 0.95 to 8.68 mg/100 g on a dry weight basis (dwb) while Zn content ranged from 0.87 to 3.8 mg/100 g (dwb). Using MLM and FarmCPU models, we found 10 significant SNPs out of which one SNP on chromosome 11 was associated with the variation in Fe content and one SNP on chromosome 4 was associated with the Zn content, and both were commonly detected by the two models. Candidate genes belonging to transcription regulator activities, including the bZIP family genes and MYB family genes, as well as transporter activities involved in Fe and Zn homeostasis were identified in the vicinity of the SNP markers and selected. The identified SNP markers hold promise for marker-assisted selection in rice breeding programs aimed at enhancing Fe and Zn content in rice. This study provides valuable insights into the genetic factors controlling Fe and Zn uptake and their transport and accumulation in rice, offering opportunities for developing biofortified rice varieties to combat malnutrition among rice consumers. Full article
(This article belongs to the Section Plant Genetics and Genomics)
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21 pages, 1333 KiB  
Article
Foliar Diseases and the Associated Fungi in Rice Cultivated in Kenya
by Everlyne M. Nganga, Martina Kyallo, Philemon Orwa, Felix Rotich, Emily Gichuhi, John M. Kimani, David Mwongera, Bernice Waweru, Phoebe Sikuku, David M. Musyimi, Samuel K. Mutiga, Cathrine Ziyomo, Rosemary Murori, Lusike Wasilwa, James C. Correll and Nicholas J. Talbot
Plants 2022, 11(9), 1264; https://doi.org/10.3390/plants11091264 - 7 May 2022
Cited by 6 | Viewed by 4658
Abstract
We conducted a survey to assess the occurrence and severity of rice blast and brown spot diseases on popular cultivars grown in the Busia, Kirinyaga, and Kisumu counties of Kenya in 2019. Working with agricultural extension workers within rice production areas, we interviewed [...] Read more.
We conducted a survey to assess the occurrence and severity of rice blast and brown spot diseases on popular cultivars grown in the Busia, Kirinyaga, and Kisumu counties of Kenya in 2019. Working with agricultural extension workers within rice production areas, we interviewed farmers (n = 89) regarding their preferred cultivars and their awareness of blast disease, as this was the major focus of our research. We scored the symptoms of blast and brown spot and assessed the lodging, plant height, and maturity of the crops (days after planting). Furthermore, we collected leaf and neck tissues for the assessment of the prevailing fungal populations. We used specific DNA primers to screen for the prevalence of the causal pathogens of blast, Magnaporthe oryzae, and brown spot, Cochliobolus miyabeanus, on asymptomatic and symptomatic leaf samples. We also conducted fungal isolations and PCR-sequencing to identify the fungal species in these tissues. Busia and Kisumu had a higher diversity of cultivars compared to Kirinyaga. The aromatic Pishori (NIBAM 11) was preferred and widely grown for commercial purposes in Kirinyaga, where 86% of Kenyan rice is produced. NIBAM108 (IR2793-80-1) and BW196 (NIBAM 109) were moderately resistant to blast, while NIBAM110 (ITA310) and Vietnam were susceptible. All the cultivars were susceptible to brown spot except for KEH10005 (Arize Tej Gold), a commercial hybrid cultivar. We also identified diverse pathogenic and non-pathogenic fungi, with a high incidence of Nigrospora oryzae, in the rice fields of Kirinyaga. There was a marginal correlation between disease severity/incidence and the occurrence of causal pathogens. This study provides evidence of the need to strengthen pathogen surveillance through retraining agricultural extension agents and to breed for blast and brown spot resistance in popular rice cultivars in Kenya. Full article
(This article belongs to the Collection Feature Papers in Plant Protection)
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16 pages, 2103 KiB  
Article
Performance as Intersectional Resistance: Power, Polyphony and Processes of Abolition
by Omid Tofighian, Rachael Swain, Dalisa Pigram, Bhenji Ra, Chandler Connell, Emmanuel James Brown, Feras Shaheen, Issa El Assaad, Luke Currie-Richardson, Miranda Wheen, Czack (Ses) Bero and Zachary Lopez
Humanities 2022, 11(1), 28; https://doi.org/10.3390/h11010028 - 17 Feb 2022
Cited by 3 | Viewed by 3497
Abstract
Australia’s brutal carceral-border regime is a colonial system of intertwining systems of oppression that combine the prison-industrial complex and the border-industrial complex. It is a violent and multidimensional regime that includes an expanding prison industry and onshore and offshore immigration detention centres; locations [...] Read more.
Australia’s brutal carceral-border regime is a colonial system of intertwining systems of oppression that combine the prison-industrial complex and the border-industrial complex. It is a violent and multidimensional regime that includes an expanding prison industry and onshore and offshore immigration detention centres; locations of cruelty, and violent sites for staging contemporary politics and coloniality. This article shares insights into the making of a radical intersectional dance theatre work titled Jurrungu Ngan-ga by Marrugeku, Australia’s leading Indigenous and intercultural dance theatre company. The production, created between 2019–2021, brings together collaborations through and across Indigenous Australian, Kurdish, Iranian, Palestinian, Filipino, Filipinx, and Anglo settler performance, activism and knowledge production. The artistic, political and intellectual dimensions of the show reinforce each other to interrogate Australia’s brutal carceral regime and the concept of the border itself. The article is presented in a polyphonic structure of expanded interviews with the cast and descriptions of the resulting live performance. It identifies radical ways that intersectional and trans-disciplinary performances can, as an ‘act of liberation’, be applied to make visible, embody, address, and help dismantle systems of oppression, control and subjugation. Full article
(This article belongs to the Special Issue Acts of Liberation)
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13 pages, 3981 KiB  
Article
Are Landscape Restoration Interventions Sustainable? A Case for the Upper Tana Basin of Kenya
by Fred Kizito, Jane Gicheha, Abdul Rahman Nurudeen, Lulseged Tamene, Kennedy Nganga and Nicholas Koech
Sustainability 2021, 13(18), 10136; https://doi.org/10.3390/su131810136 - 10 Sep 2021
Cited by 2 | Viewed by 2489
Abstract
Landscape restoration initiatives often have the potential to result in environmental gains, but the question of whether these gains are sustainable and how they are linked to other community needs (social, productivity and economic gains) remains unclear. We use the Sustainable Intensification Assessment [...] Read more.
Landscape restoration initiatives often have the potential to result in environmental gains, but the question of whether these gains are sustainable and how they are linked to other community needs (social, productivity and economic gains) remains unclear. We use the Sustainable Intensification Assessment Framework (SIAF) to demonstrate how environmental benefits are linked to productivity, environment, social, human, and economic components. Using the SIAF, the standardization of relevant indicators across multiple objectives provided a contextual representation of sustainability. The study assessed the overall gains resulting from the measured indicators of sustainable land management (SLM) practices and their relationship to the multiple domains of the SIAF. We present a unique case for SLM options using a combined-methods approach where biophysical, socio-economic, and citizen science help assess the sustainability of the interventions. Using a participatory approach with farmers, land restoration options were conducted in four target micro-watersheds for 3 years (2015–2017). Co-developed restoration measures at the landscape level within the four micro-watersheds (MW1-MW4) resulted in a substantial increment (50%) for all treatments (grass strips, terraces, and a combination of grass strips and terraces) in soil moisture storage and increased maize and forage production. We demonstrate that SLM practices, when used in combination, greatly reduce soil erosion and are profitable and sustainable while conferring livelihood benefits to smallholder farmers. Full article
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16 pages, 1736 KiB  
Article
Multi-Environmental Evaluation of Protein Content and Yield Stability among Tropical Soybean Genotypes Using GGE Biplot Analysis
by Tonny Obua, Julius Pyton Sserumaga, Bruno Awio, Fredrick Nganga, Thomas L. Odong, Phinehas Tukamuhabwa, Geoffrey Tusiime, Settumba B. Mukasa and Margaret Nabasirye
Agronomy 2021, 11(7), 1265; https://doi.org/10.3390/agronomy11071265 - 22 Jun 2021
Cited by 9 | Viewed by 3166
Abstract
The yield and protein performance in a soybean genotype result from its interaction with the prevailing environmental conditions. This makes selecting the best genotypes under varied target production environments more complex. This study’s objectives were to determine protein content and protein stability of [...] Read more.
The yield and protein performance in a soybean genotype result from its interaction with the prevailing environmental conditions. This makes selecting the best genotypes under varied target production environments more complex. This study’s objectives were to determine protein content and protein stability of 30 elite soybean genotypes in major soybean-growing areas of Uganda, assess the yield performance and stability in soybeans and determine the relationship between the protein content and grain yield in soybeans. The genotypes were planted in a randomized complete block design of three replications for six seasons across eight locations in Uganda. Genotype and genotype-by-environment (GGE) biplot analyses classified the test locations into three mega-environments for soybean protein and grain yields. Genotype NII X GC 20.3 had the highest mean protein content of 43.0%, and BSPS 48A-9-2 and BSPS 48A-28 were superior for the mean grain yield (1207 kg ha−1). Bulindi was the most discriminating and representative test environment for soybean yield. A weak and negative correlation (r = −0.1**, d.f. = 29) was detected between the protein content (%) and yield (kg ha1). The highest-yielding genotypes BSPS 48A-9-2, BSPS 48A-31, and Nam II × GC 44.2 are recommended for further evaluation under farmers’ production conditions for selection and release as new soybean varieties in Uganda. Full article
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29 pages, 2560 KiB  
Review
Polysaccharides and Derivatives from Africa to Address and Advance Sustainable Development and Economic Growth in the Next Decade
by Antony Sarraf, Emeline Verton, Noura Addoun, Zakaria Boual, Mohamed Didi Ould El Hadj, Zainab El Alaoui-Talibi, Cherkaoui El Modafar, Slim Abdelkafi, Imen Fendri, Cédric Delattre, Pascal Dubessay, Philippe Michaud and Guillaume Pierre
Appl. Sci. 2021, 11(11), 5243; https://doi.org/10.3390/app11115243 - 4 Jun 2021
Cited by 5 | Viewed by 4485
Abstract
Polysaccharides are highly variable and complex biomolecules whose inventory of structures is still very incomplete, as nature still preserves unexplored biotopes. Plants, macroalgae and microalgae are an integral part of the daily life of human being regardless of culture, time, or knowledge development [...] Read more.
Polysaccharides are highly variable and complex biomolecules whose inventory of structures is still very incomplete, as nature still preserves unexplored biotopes. Plants, macroalgae and microalgae are an integral part of the daily life of human being regardless of culture, time, or knowledge development of a country. Natural medicine is an ancestral knowledge widely distributed throughout the world, handed down for centuries from generation to generation by those commonly referred to as “nganga” healers or shamans. It is also called alternative medicine or traditional medicine, and has been associated for millennia to legends. This review gives an emphasis regarding the ethnobotanic approach associated to the structural variability of poly- and oligosaccharides for designing the new polysaccharide-based drugs and hydrocolloids of tomorrow. The guiding thread is to survey the potential of plants (and some macroalgae) from Africa as a source of polysaccharides with original structures and, secondly, to correlate these structures with biological and/or functional properties in particular to address and advance the sustainable development and economic growth of mankind. Full article
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16 pages, 13796 KiB  
Article
Influence of Heat Treatments, Parameters and Machining Conditions on Machinability in the Turning of Recycled 6061 Aluminum Alloy
by Jean Brice Mandatsy Moungomo, Guy Richard Kibouka and Donatien Nganga-Kouya
J. Manuf. Mater. Process. 2021, 5(2), 37; https://doi.org/10.3390/jmmp5020037 - 14 Apr 2021
Cited by 3 | Viewed by 3287
Abstract
Some manufacturing companies now use recycled aluminum alloys. It is important that they have the necessary data relating to the control of the machinability of these alloys. Thus, this study on the machinability in the turning of two recycled aluminum alloys by a [...] Read more.
Some manufacturing companies now use recycled aluminum alloys. It is important that they have the necessary data relating to the control of the machinability of these alloys. Thus, this study on the machinability in the turning of two recycled aluminum alloys by a 6061 R and 6061 R-T6 smelter was conducted. The aim of this study is to provide solutions to the problem posed, which is whether recycled aluminum alloys have good machinability skills, such as virgin aluminum alloys. To provide these solutions, the experimental designs were used to study the influence of cutting parameters and conditions (feed, cutting speed, lubrication) and material hardness on machinability characteristics (surface roughness, mass concentration of metal particles, and chip morphology). The results of this study show that the two alloys studied have good machinability. The feed, hardness and lubrication significantly influence the machinability of these two alloys. Predictive models to assess the machinability of these recycled alloys have been established. Full article
(This article belongs to the Special Issue Accuracy Improvements in Machine Tools and Machining)
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8 pages, 239 KiB  
Article
Mpambu Nzila: José Bedia at the Crossroads
by Alan West-Durán
Religions 2021, 12(3), 172; https://doi.org/10.3390/rel12030172 - 6 Mar 2021
Cited by 2 | Viewed by 2946
Abstract
The article draws on the Kongo term mpambu nzila of crossroads, that equally signifies altar, to discuss the paintings and drawings of Cuban-born artist José Bedia. He is a practitioner of Palo Briyumba, a syncretic Afro-Cuban religion that combines Kongo religious beliefs, Regla [...] Read more.
The article draws on the Kongo term mpambu nzila of crossroads, that equally signifies altar, to discuss the paintings and drawings of Cuban-born artist José Bedia. He is a practitioner of Palo Briyumba, a syncretic Afro-Cuban religion that combines Kongo religious beliefs, Regla de Ocha, Spiritism, and Catholicism. The article examines six works by the artist from 1984 to 1999 and how Bedia represents Palo in his art. Additionally, the centrality of the nganga (a cauldron that paleros use to work for and protect them) is discussed historically, philosophically, and religiously as a physical and spiritual embodiment of the crossroads. Bedia’s work is also analyzed using the Sankofa bird as metaphor (of flying forward and looking back) and as an example of the West African notion of coolness. The article also examines Palo as a de-colonial way of knowing and ends with the crossroads through the example of Lucero Mundo (Elegguá). Full article
(This article belongs to the Special Issue Syncretism and Liminality in Latin American and Latinx Religions)
19 pages, 1382 KiB  
Article
Assessment of Fungal Contamination in Fish Feed from the Lake Victoria Basin, Uganda
by Victoria Tibenda Namulawa, Samuel Mutiga, Fred Musimbi, Sundy Akello, Fredrick Ngángá, Leah Kago, Martina Kyallo, Jagger Harvey and Sita Ghimire
Toxins 2020, 12(4), 233; https://doi.org/10.3390/toxins12040233 - 7 Apr 2020
Cited by 20 | Viewed by 5109
Abstract
The emergence of commercial fish farming has stimulated the establishment of fish feed factories in Uganda. However, no information is available on the safety of the feed, mainly due to lack of mycotoxin testing facilities and weak regulatory systems. A study was carried [...] Read more.
The emergence of commercial fish farming has stimulated the establishment of fish feed factories in Uganda. However, no information is available on the safety of the feed, mainly due to lack of mycotoxin testing facilities and weak regulatory systems. A study was carried out to examine fungal colonization and mycotoxin contamination in fish feed samples (n = 147) of different types collected from nine fish farms (n = 81) and seven fish feed factories (n = 66) in the Lake Victoria Basin (LVB). Fungi were isolated in potato dextrose agar, grouped into morphotypes and representative isolates from each morphotype were identified based on the internal transcribed spacer (ITS) region of ribosomal DNA sequences. Aflatoxin B1 (AFB1) and total fumonisin (combinations of B1, B2 and B3; hereinafter named fumonisin) levels in feed samples were determined by enzyme-linked immunosorbent assay (ELISA). A wide range of fungi, including toxigenic Aspergillus flavus and Fusarium verticillioides, were isolated from the fish feed samples. AFB1 was detected in 48% of the factory samples and in 63% of the farm samples, with toxin levels <40 and >400 µg/kg, respectively. Similarly, 31% of the factory samples and 29% of the farm samples had fumonisin contamination ranging between 0.1 and 4.06 mg/kg. Pellets and powder had higher mycotoxin contamination compared to other commercially available fish feed types. This study shows AFB1 as a potential fish feed safety issue in the LVB and suggests a need for more research on mycotoxin residues in fish fillets. Full article
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19 pages, 2627 KiB  
Article
Genetic Profiling of Aspergillus Isolates with Varying Aflatoxin Production Potential from Different Maize-Growing Regions of Kenya
by Richard Dooso Oloo, Sheila Okoth, Peter Wachira, Samuel Mutiga, Phillis Ochieng, Leah Kago, Fredrick Nganga, Jean-Baka Domelevo Entfellner and Sita Ghimire
Toxins 2019, 11(8), 467; https://doi.org/10.3390/toxins11080467 - 9 Aug 2019
Cited by 23 | Viewed by 7137
Abstract
Highly toxigenic strains of Aspergillus flavus have been reported to frequently contaminate maize, causing fatal aflatoxin poisoning in Kenya. To gain insights into the environmental and genetic factors that influence toxigenicity, fungi (n = 218) that were culturally identified as A. flavus [...] Read more.
Highly toxigenic strains of Aspergillus flavus have been reported to frequently contaminate maize, causing fatal aflatoxin poisoning in Kenya. To gain insights into the environmental and genetic factors that influence toxigenicity, fungi (n = 218) that were culturally identified as A. flavus were isolated from maize grains samples (n = 120) from three regions of Kenya. The fungi were further characterized to confirm their identities using a PCR-sequence analysis of the internal transcribed spacer (ITS) region of rDNA which also revealed all of them to be A. flavus. A subset of 72 isolates representing ITS sequence-based phylogeny cluster and the agroecological origin of maize samples was constituted for subsequent analysis. The analysis of partial calmodulin gene sequences showed that the subset consisted of A. flavus (87%) and Aspergillus minisclerotigenes (13%). No obvious association was detected between the presence of seven aflatoxin biosynthesis genes and fungal species or region. However, the presence of the aflD and aflS genes showed some association with aflatoxin production. The assessment of toxigenicity showed higher aflatoxin production potential in A. minisclerotigenes isolates. Given that A. minisclerotigenes were mainly observed in maize samples from Eastern Kenya, a known aflatoxin hotspot, we speculate that production of copious aflatoxin is an adaptative trait of this recently discovered species in the region. Full article
(This article belongs to the Collection Aflatoxins)
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