Search Results (533)

This study evaluated the effects of incorporating potential probiotic strains into Italian-style salami. Three lactic acid bacteria (LAB) strains were tested, including the commercial strain Pediococcus pentosaceus co-cultured with Staphylococcus xylosus (C), as well as the LAB strains Lacticaseibacillus paracasei (GV17), Lactococcus lactis (GV103), and Pediococcus pentosaceus (PP). Preliminary tests were conducted to assess the behavior of these strains under different pH values, NaCl concentrations, and preservative conditions. C, GV17, and GV103 exhibited greater viability at pH 5.0 and 5.5, while a 2% NaCl concentration promoted higher LAB growth rates. The addition of preservatives did not inhibit LAB growth. Weight loss during ripening was gradual and became significant from day 12 onward. GV17 and PP exhibited water activity values above 0.90, whereas all four formulations reached moisture contents below 35% and LAB counts above 8.00 log CFU/g after 25 days of ripening. After 45 days of storage, the LAB count in the GV17 formulation was 6.27 log CFU/g. PP exhibited the lightest internal color among the salami formulations, whereas red intensity was similar across all formulations. The use of autochthonous LAB strains significantly reduced hardness and chewiness. Overall, the new LAB strains demonstrated potential for the development of functional meat products, expanding opportunities for innovation in the food sector. Full article

In aquaculture, reduced water exchange helps constrain exogenous pathogen invasion, lower pumping costs, and improve management efficiency; nevertheless, its effects on the growth of cultured aquatic animals remain poorly characterized. This study compared two daily water-exchange rates (100% and 0%) to evaluate growth and associated intestinal responses in Babylonia areolata (ivory shell). Growth performance and intestinal enzyme activities were measured, while 16S rRNA gene sequencing and untargeted metabolomics were used to characterize microbial composition and metabolite profiles. The low-water-exchange group exhibited significantly lower weight gain rate (WGR, p < 0.0001), specific growth rate (SGR, p < 0.0001), daily increments in shell length (DISL, p = 0.0060), and shell width (DISW, p = 0.0012). Intestinal total superoxide dismutase (T-SOD, p < 0.0001) and lipase (LIP, p = 0.0011) activities were also significantly lower. The relative abundances of Vibrio, Halodesulfovibrio, and Fusibacter were higher, whereas that of Christensenellaceae_R-7_group was lower. Lipid-related metabolic pathways showed the most pronounced intestinal differences. Intestinal levels of prostaglandins (PGs), leukotrienes (LTs), and sphingosine (Sph) were significantly higher under low-water-exchange conditions. The relative abundance of Halodesulfovibrio correlated positively with PGs and LTs but negatively with lysophospholipids (Lpc) and T-SOD activity. The relative abundance of Christensenellaceae_R-7_group correlated positively with LIP activity and was significantly associated with metabolites involved in primary bile acid biosynthesis. Overall, low-water-exchange conditions were associated with coordinated changes in growth, intestinal enzyme activities, microbial composition, and metabolite profiles, although the underlying mechanisms require functional validation. These findings provide new insights into intestinal microbiota–metabolite responses to low-water-exchange conditions and may guide future validation and intervention studies in RAS-based ivory shell culture. Full article

What happens to emotional life when running water fails not for a day but for a decade? Drawing on 20 semi-structured interviews in a rural Romanian commune that went more than ten years without a functioning public water supply, and where a new network now reaches only part of the settlement, we examine how the emotional demands of chronic water scarcity are socially organised. We identify four recurrent patterns, which we analyse as feeling rules: shame before visiting outsiders, worry tied to falling household reserves, hope maintained in public, and resignation expressed in contained, formulaic terms. The four carry unequal normative warrant, which we assess explicitly. We propose the concept of infrastructural emotion work, the unpaid emotion work generated by the sustained failure of systems socially expected to function, and identify formulaic emotional display as one of its mechanisms, in which a conventional phrase provides an interactionally acceptable response while foreclosing elaboration. We argue that these expectations are socio-materially generated rather than culturally prescribed alone, and that the resulting burdens are unevenly distributed by gender. Where infrastructure is unreliable, households assemble an emotional infrastructure of their own. Full article

This article offers a fresh reading of Alonso de Oropesa’s Lumen ad revelationem gentium (1465) and of the pro-converso theology of this Hieronymite friar, through a close reading of a specific and largely neglected section of the treatise. This reading complicates the prevailing scholarly view that the Lumen expresses an essentially egalitarian theological vision. The article argues that Oropesa’s intensely anti-Jewish stance and emphasis on Pauline spirituality were part of a more layered and ambitious argument—one that sought to establish a genuine continuity between Judaism and Christianity through a creative reinterpretation of Paul and his conversion. Drawing on a highly original reading of the ice-water metaphor from Gregory the Great’s Moralia in Job and the letter-spirit binary of II Corinthians 3:6, Oropesa articulated a bold vindication of Jewish heritage as a worthy and enduring component of Christianity’s own fabric—preserved, rather than erased, through spiritual transformation. The article further suggests that this theological vision extended beyond the realm of ideas into that of practice, offering a new background for understanding the devotional culture of Hieronymite conversos and its eventual collision with the Inquisition. Full article

Urban vitality is a key indicator for characterizing the quality of urban space and the operational status of urban functions. However, existing studies still have limitations in multidimensional vitality measurement at the block scale, the representation of hierarchical differences in cultural facilities, and the coupling coordination diagnosis of multidimensional vitality. This study takes 2504 blocks in the central urban area of Nanjing as the basic analytical units and integrates multi-source geospatial data, including VIIRS nighttime light data, Baidu Huiyan population heat data, POIs, road networks, and water systems, to construct a three-dimensional urban vitality evaluation system encompassing economic, social, and cultural vitality. A Composite Nighttime Light Index (CNLI) is constructed by geometrically fusing VIIRS nighttime light data with the kernel density of industry- and consumption-related POIs to reduce the impact of the spatial generalization of nighttime lights on block-scale economic vitality measurement. Meanwhile, population heat data and cultural POIs are used to characterize social vitality and cultural resource supply, respectively, and PCA, a coupling coordination model, and spatial autocorrelation analysis are combined to identify the spatial structure of multidimensional vitality and the dominant factors of disorder. External reference variables are also introduced to conduct convergent validity verification. The results indicate that the comprehensive vitality of Nanjing’s central urban area exhibits a distinct “core agglomeration–multi-node diffusion” structure. High-vitality zones are primarily concentrated in Xinjiekou, Confucius Temple, Hunan Road–Zhongyang Road, Longjiang, and the Nanjing Olympic Sports Center, with localized vitality patches forming at peripheral commercial and transportation nodes. Both comprehensive vitality and coupling coordination degree exhibit significant positive spatial autocorrelation, with Moran’s I values of 0.8089 and 0.8372, respectively. The disorder types show distinct quantitative differences and spatial differentiation. Among these, blocks with lagging cultural vitality are the most numerous; peripheral new towns and newly developed residential areas are more prone to cultural vitality lag; areas surrounding scenic spots, universities, and large ecological spaces tend to exhibit economic vitality lag; and less developed peripheral blocks primarily exhibit comprehensive disorder. Based on accessible multi-source geospatial data, this study constructs a block-scale framework for measuring multidimensional urban vitality and diagnosing coordination status. This framework can provide a reference for vitality identification, functional shortcoming diagnosis, and refined spatial governance in Nanjing’s central urban area, and offer a case reference for historic and cultural cities with similar spatial structures. Full article

Hydrological conditions in the Mackenzie River Basin (MRB) are becoming increasingly variable due to climate change, permafrost degradation, and cumulative industrial impacts. While scientific assessments have documented many of these trends, far less is known about how changing water levels and flow patterns affect the daily lives, livelihoods, and cultural responsibilities of Indigenous Peoples across the Basin. This paper synthesizes basin wide Indigenous Knowledge related to water level and flow variability, drawing on 31 Indigenous-led research projects. The analysis highlights shared concerns across regions, including reduced travel safety, restricted access to harvesting areas, shifting river and lake behaviour, and emotional and spiritual impacts associated with hydrological extremes. These observations align with scientific evidence of earlier breakup, prolonged low-water periods, and increased hydrological unpredictability, while also revealing social and cultural dimensions not captured through conventional monitoring. By synthesizing basin wide Indigenous Knowledge of water level and flow variability, this study provides new insight into the cumulative social ecological consequences of freshwater change in the MRB and underscores the importance of Indigenous-led research and governance in responding to accelerating hydrological variability. Full article

Many species of actinomycete bacteria synthesise glycosylated polyene macrolides that have potential as antifungal drugs. The sugar residues of these compounds have profound effects on potency, toxicity and water-solubility. The medically important antibiotics amphotericin B and nystatin A1 have a single D-mycosamine sugar on C19 of the polyene macrolactone. A few naturally occurring polyenes have a second sugar residue. This may be attached to C35 of 38-membered macrolactones like nystatins or to the equivalent C27 of 30-membered pentaenes like selvamicin. The recently discovered mandimycin has a C35 disaccharide that changes the mode of action, reduces adverse side effects, and delays the emergence of resistance in laboratory cultures of fungal pathogens. Glycosyltransferases that can modify the C27 and C35 positions are of interest to synthetic biologists. The GloSV enzyme is predicted to add a 2,6-dideoxy-D-hexose to C27 of a pentaene in Saccharopolyspora gloriosae. Here we assess GloSV in strains of the amphotericin producer, Streptomyces nodosus. Low levels of new amphotericin analogues modified with D-oliose or D-digitoxose were identified through HR-LCMS. The identification of this glycosyltransferase will assist the development of streptomycete systems for production of non-toxic polyene glycoanalogues. Full article

Advanced methodologies for Botryllus schlosseri artificial seawater systems are needed to decrease dependency of large-scale culture on natural seawater and expand this important new model organism to more inland laboratories. We constructed two botryllid tunicate customized closed aquaculture systems, a static system consisting of aerated jars fed commercial filter feeder diet, and a recirculating aquaculture system (RAS) consisting of pertinent marine RAS components fed live microalgae and zooplankton diets. Initially, static tunicate culture yielded exponential growth in contrast to poor survival and negligible growth observed in RAS tunicates. RAS modifications were made to increase water treatment proficiency, which improved tunicate survival and growth. Experiments were performed isolating feed and water type as variables differentiating static and RAS and evaluating their specific effects. Live feed promoted five-fold greater growth relative to a commercial concentrate diet. Tunicates maintained in optimized RAS water achieved two-fold faster growth relative to animals in freshly prepared artificial seawater. Subsequent procedural modifications combined with the RAS revisions resulted in growth rates comparable to the static system. Both optimized systems are suitable for long-term husbandry of botryllid tunicate populations supporting both sexual and asexual modes of reproduction, with a current RAS residence time of over 24 months. Full article

The middle reaches of the Minjiang River, shaped by the Dujiangyan irrigation system, provide a typical setting for studying long-term human–water interactions. During the Little Ice Age, the water management system as a whole experienced a full cycle of recovery, expansion, and decline from 1644 to 1949 (Qing to Republican period), although subregions exhibited marked spatial heterogeneity. This heterogeneity makes the area an ideal case for comparative analysis; however, previous studies have neither quantitatively reconstructed river–canal changes nor systematically disentangled the composite natural and anthropogenic drivers across different subregions. Using archival documents, historical maps, remote sensing imagery, and water cultural heritage sites, this study reconstructs the evolution and quantifies two change types: anthropogenic construction, including new construction, reconstruction, and modification, and environmentally driven changes such as rerouting, damage, and maintenance. Correlations were analyzed among the four subregions: Inner River, Outer River, Nanhe River, and the Lower Basin to identify driving mechanisms. Results indicate that anthropogenic construction is constrained by natural conditions and driven by population growth, whereas environmentally driven changes are primarily caused by floods and worsened by canal head maintenance failure. The four spatially differentiated driving patterns are: Inner River—human-dominated intervention type; Outer River—flood stress type; Nanhe River—low-disturbance stable type; and Lower Basin—natural–human composite type. This study offers new insights into long-term human–water interactions in large irrigation districts under climate change. Full article

Tomato (Solanum lycopersicum) is a globally important horticultural crop, with Asia contributing 60.45% of total production, followed by the Americas at 13.36%. Tomato productivity is increasingly constrained by southern blight, a destructive disease responsible for yield losses ranging from 30 to 90% and annual economic damage of $10–20 million. The causal pathogen, Sclerotium rolfsii, infects the stem base and induces reddish-brown cankers through secretion of oxalic acid (OA) and cell wall-degrading enzymes, which girdle tissues, impair water transport, and result in rapid plant wilting and death. Its persistence in soil via sclerotia, broad host range, and adaptability make the disease difficult to manage. Recent advances in genomics, transcriptomics, proteomics and other multi-omics approaches have substantially improved understanding of pathogen virulence factors, host defense responses and disease epidemiology. These studies have revealed key roles of OA, carbohydrate-active enzymes, effector proteins, and sclerotial melanization in pathogenesis, while highlighting the activation of salicylic acid (SA)-, jasmonic acid (JA)-, and ethylene (ET)-mediated defense pathways in tomato. Although cultural, biological, and chemical measures are available, these measures often provide inconsistent protection when used alone. Promising strategies include the use of biocontrol agents, hypovirulence-inducing mycoviruses, and chemical fungicides such as carboxamides and quinone outside inhibitors (QoIs), though fungicide resistance remains a risk factor. Integrated Disease Management (IDM) approaches, such as combining biocontrol agents with fungicides, demonstrate enhanced efficacy. This review also evaluates progress in resistance breeding, grafting, RNA interference (HIGS and SIGS), CRISPR-based genome editing, and exploitation of wild genotypes for durable resistance. Furthermore, emerging precision agriculture tools, including hyperspectral imaging, machine learning-assisted disease detection and climate-resilient management strategies, were discussed as new components of sustainable disease management. Full article

In citrus production, there is an absence of established standards of critical Nickel (Ni) content for deficiency, sufficiency, and excess, which could be used to determine the nutritional status of plant Ni. In this study, to explore the critical Ni concentrations for deficiency and excess, we conducted a hydroponic pot culture experiment and investigated the effects of Ni levels on flower and fruit development, dry weight, and nutrient accumulation of Newhall navel orange. We found that 0.8 and 6.4 mg L⁻¹ of solution Ni were the turning point concentrations of Ni deficiency and excess for plants, respectively. Solution Ni deficiency (0 to 0.8 mg L⁻¹ of Ni) tended to promote vegetative growth and increase the dry weight of new leaves, but suppress flower bud number and fruit development. It also significantly promoted the accumulation of N, P, K, Ca, and Mg in old leaves and N and K in roots, but significantly reduced that of Fe, Mn, and Zn in roots. Excess solution Ni (6.4 to 12.8 mg L⁻¹ of Ni) reduced the water content of fruit peel and was accompanied by fruit cracking during the fruit expansion period, inhibited new leaf growth and whole plant biomass or dry weight, and significantly decreased nutrient accumulation in roots. Equations of dry weight and solution Ni levels for each plant organ were established, showing that 3.93 to 4.72 mg L⁻¹ of Ni was the sufficient concentration of solution Ni for the growth and development of Newhall navel orange, with the corresponding range of Ni contents in new and old leaves being 17.87 to 20.42 and 10.24 to 11.64 mg kg⁻¹, respectively. These findings provide reference for the recommended range of Ni sufficient for citrus growth. Full article

The presence of xenobiotics in wastewater, particularly emerging contaminants such as pharmaceuticals, poses an ecotoxicological risk to the environment and human health. One of the main pharmaceutical products detected in water is diclofenac, which can be sold without a prescription. The lack of health regulations indicates the necessity of finding environmentally friendly treatment alternatives to remove this type of contaminant. Among these alternatives, biotechnology, specifically biological processes, offers a sustainable option compared to conventional treatments. Current treatment methods used in wastewater treatment plants are ineffective at removing diclofenac, a chlorinated aromatic compound highly resistant to degradation processes. In recent years, new treatment methods have gained prominence due to the favorable results they have yielded, including physicochemical, biological, and advanced processes. Biological treatments are notable for their low cost and the high level of effectiveness and efficiency with which they can remove toxic compounds. For this reason, the aim of this research project was to evaluate the degradation efficiency of a biological treatment in a bioreactor using a microbial community consisting of five bacterial strains, which was isolated from a pharmaceutical effluent and cultivated in a continuous culture system. Removal efficiencies ranging from 99.38 to 99.98% were achieved at various volumetric loading rates (from 0.087 to 1.043 g L⁻¹d⁻¹). Influents and effluents from the biological reactor were analyzed using bioassays to determine any potential toxic effects. The results showed that the effluents did not elicit a negative response in the bioindicators, indicating high toxicity in the influents. Full article

The detection and degradation analysis of subsurface microcracks in mural paintings remain challenging due to their inhomogeneous multilayered structure and complex deterioration mechanisms. In this study, we propose a multimodal stepwise method for three-dimensional characterization and cross-scale degradation analysis by integrating digital holography (DH), infrared thermography (IRT), acoustic excitation (AE), and molecular dynamics (MD) simulations. In the first step, an adjustable field-of-view (FOV) digital holographic system is developed to capture subsurface deformation under acoustic excitation, enabling high-resolution planar characterization of subsurface microcracks. Infrared thermography is then employed to estimate crack depth through an inverse thermal model, achieving full three-dimensional reconstruction of crack geometry. Based on the reconstructed structures, MD simulations are conducted to investigate the evolution of stress, bond breaking, and crack propagation under varying temperature and humidity conditions, with particular emphasis on water molecule migration and chemically induced degradation. The results demonstrate that environmental factors promote stress concentration and material embrittlement at crack tips, leading to secondary microcrack formation and progressive deterioration. Experimental aging tests show strong agreement with simulation results, validating the proposed methodology. This work establishes a unified “characterization–simulation–validation” paradigm, providing new insights into the mechanisms of mural degradation and offering a robust framework for non-destructive evaluation and preventive conservation of multilayer cultural heritage materials. Full article

Arbuscular mycorrhizal (AM) fungi form mutualistic symbioses with about 80% of land plants and play a key role in improving host inorganic phosphate (Pi), nitrogen, and water acquisition. Traditional AM fungi research relies on field trials, compartmented cultivation, and pot cultures—methods that are time-consuming (taking months to years) and unable to monitor dynamic transport, thus limiting efficient strain screening. We developed a real-time fluorescence imaging platform integrating sterile symbiotic microchambers with photodiode array detection. This system enables the non-invasive, quantitative tracking of hyphal cytoplasmic streaming and transport kinetics at the plant–fungal interface. Distinct AM fungi strains exhibit significant differences in fluorescence kinetics—such as accumulation rate and peak intensity—providing measurable indicators of transport efficiency. Our method overcomes the temporal and technical limitations of conventional AM fungi screening approaches. By enabling simultaneous real-time monitoring, it shortens screening cycles and provides new insights for the (1) precise screening of AM fungi strains for efficient nutrient transport; (2) investigation of nutrient exchange mechanisms; (3) development of sustainable microbial inoculants. Full article

Members of the class Campylobacteria are microaerophilic bacteria widely distributed across diverse environments and are abundant in hydrothermal systems. However, cultivated representatives, particularly from shallow-water vents, remain limited. Here, we investigated the genomic diversity and environmental adaptation of the genus Sulfurospirillum. Phylogenomic analysis revealed a clear separation between terrestrial and marine clades, with relatively few cultured representatives in the marine lineage. Strain 1307, isolated from shallow-water hydrothermal vents, expands the genomic representation of this underexplored clade. Pan-genome analyses based on complete genomes revealed an open pan-genome, indicating ongoing diversification of genus Sulfurospirillum. Further comparison between hydrothermal vent (HTV) and non-HTV lineages identified distinct adaptive features. Vent-associated strains are enriched in genes involved in sulfur metabolism, carbon fixation, the glycine cleavage system (GCS), and the biosynthesis of key cofactors (spermidine, thiamine, lipoate, and heme), reflecting metabolic adaptation to hydrothermal environments. Beyond well-established processes such as sulfur metabolism and autotrophic carbon fixation, the widespread presence of the GCS in vent-associated lineages suggests its potential role as an auxiliary carbon fixation pathway under anaerobic conditions. Overall, this study expands the phylogenetic and genomic diversity of Sulfurospirillum and offers new insights into the mechanisms underlying environmental adaptation and niche differentiation in vent-associated Campylobacteria. Full article