Search Results (3,460)

Glucose oxidase (GOD) is a natural enzyme with antioxidant and antimicrobial properties but its effects on sows remain insufficient. This study investigated the effects of dietary GOD supplementation during gestation on inflammatory response, antioxidant capacity, immune function, and gut microbiota of farrowing sows. Twenty-four primiparous sows were randomly assigned to two groups and fed a basal diet or a basal diet supplemented with GOD (300 mg/kg diet) from gestation day 30 to farrowing. GOD supplementation significantly increased triglyceride, superoxide dismutase, and immunoglobulin M levels (p < 0.05), and significantly decreased alanine aminotransferase and interleukin-6 levels in serum (p < 0.05); significantly reduced placental interleukin-1β, malondialdehyde and tumor necrosis factor-α concentrations and NF-κB gene expression (p < 0.05), and elevated glutathione peroxidase activity and relative mRNA expressions of Nrf2, HO-1, GPX1 and SOD2 (p < 0.05). Moreover, GOD supplementation altered the fecal microbial community structure (p < 0.05), significantly reducing Clostridium, dgaA-11_gut_group, Bacteroides, and Prevotellaceae_NK3B31_group abundance (p < 0.05), while enriching Lachnospira, unclassified_f_Erysipelotrichiaceae, and Anaerostipes (p < 0.05). Collectively, 300 mg/kg glucose oxidase supplementation during mid-to-late gestation improved the health status of farrowing sows by improving nutrient utilization, immune function and antioxidant capacity, and altering fecal microbial structure and relative abundances. Full article

An essential multiprotein complex in the innate immune system is the NOD-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome. Through the coordination of upstream sensors and the adaptor protein ASC, which converge on the inflammasome complex in response to pathogens and cellular homeostatic disruptions, a broad range of metabolic danger signals activate the NLRP3 inflammasome. New research demonstrates the anti-inflammatory qualities of natural products, suggesting that they could be used as supplemental treatments for a number of chronic inflammatory conditions, such as diabetes mellitus and cardiovascular disorders. The literature related to inhibition of the NLRP3 inflammasome was compiled using keywords such as “NLRP3 Inflammasome”, “Natural products”, and “Inhibitor” from scientific databases, including “China Knowledge Resource Integrated Databases (CNKI)”, “Google Scholar”, “PubMed”, and “Web of Science” and so on, covering the period from 1997 to 2025. Herbal remedies have shown protection against the activation of the NLRP3 inflammasome. For the treatment of chronic inflammatory illnesses, natural products’ capacity to prevent NLRP3 inflammasome activation offers a novel and practical therapeutic approach. The impact of natural compounds made from medicinal plants on NLRP3 inflammasome activation and their underlying mechanisms of action are the main topics of this review. Full article

Vibration energy is widely present in the natural environment. In the development of wearable self-powered systems, how to efficiently harvest the low-frequency mechanical energy of human motion has always been a core challenge. The piezoelectric–electromagnetic hybrid energy harvester designed in this paper consists of two units: a piezoelectric unit and an electromagnetic unit. The piezoelectric unit is composed of two arched plates, a piezoelectric layer, and an end magnet. The two sides of the piezoelectric unit are completely symmetrical. The electromagnetic unit is composed of a hollow tube, a central magnet, and a coil. The coil is wound around the outside of the center of the hollow tube to ensure that the central magnet can cut more magnetic flux lines. The two units output voltage through an external load. Firstly, based on a physical model, the force–electricity coupling mechanism is derived, and the dynamic response of the harvester at different frequencies is systematically tested. Secondly, through simulation and experiment, the influencing factors of the output voltage are deeply studied, and it is concluded that at medium and low frequencies (5 Hz–15 Hz), the harvester can provide efficient voltage output. The electromagnetic unit dominates at low frequencies and can output a larger voltage, but the voltage drops significantly after a certain frequency. The piezoelectric unit can supplement after the electromagnetic voltage drops, and the two have a synergistic effect. In addition, the output characteristics of the system mainly depend on frequency, initial distance, coil turns, and magnet mass. This paper clarifies the inherent physical mechanism of the hybrid energy harvester and provides an effective scientific reference for practical human motion energy conversion applications. Full article

Background: The use of food supplements (FS) is rapidly increasing, particularly in Italy, which leads the European market. This trend is driven by various factors, including the pursuit of physical well-being, the influence of advertising, and concerns about disease prevention. This exploratory pilot descriptive study aimed to characterize FS use among patients attending general practitioner (GP) offices and examine potential patterns with psychophysical well-being. Methods: Two questionnaires were administered to participants: one on FS use and another on physical and mental health (SF-12 questionnaire). General information and anthropometric characteristics were also collected. Results: 230 questionnaires on FS use and 192 on psychophysical well-being were analyzed. The majority of participants (73.5%) reported using FS, primarily for general well-being (21.0%), immune system support (12.2%), and increased energy (11.4%). The most commonly consumed FS were vitamins (19.4%), minerals (16.9%), and probiotics (15.7%). Only 57.4% of patients reported informing their doctor about FS use, while 66.3% engaged in self-prescription. The SF-12 questionnaire revealed lower mental health scores (mood, energy, anxiety, and depression) in the studied population, while physical health remained unaffected. Importantly, no significant associations were observed between FS use and either physical or mental health scores, suggesting these patterns are independent of supplement consumption. Conclusions: FS use is prevalent among patients attending GP offices. The observed decrease in mental health scores may indicate psychological distress though this pattern was not associated with FS consumption. Given the exploratory nature of this study, findings should be interpreted with caution. This study highlights the need for improved health education and professional training to promote safer and more informed FS use. Further research is required to expand upon these initial findings. Full article

Plant-derived peptides have become one of the most promising classes of compounds in cancer research due to their specificity, safety, and different therapeutic actions. Generally, plant peptides have a size of 2 to 100 amino acids, and they can be extracted from different parts of the plant including leaves, seeds, stems, and roots. The present review brings together more than 300 prominent plant peptides, their sources, structural classes, extraction methods, anticancer effects, and mechanisms of action. We show the cytotoxicity of plant peptides against a wide range of human cancer cell lines (such as MCF-7, A549, HL-60, and HCT-116), as well as their effectiveness in preclinical animal models of cancer, where they resulted in lesser tumor growth and metastasis. Moreover, we go into the anticancer activity of plant peptides and reveal the interconnectedness of apoptosis, cell cycle arrest, angiogenesis inhibition, metastasis suppression, and the modulation of signaling pathways as some of the mechanisms through which plant peptides perform. In addition to their therapeutic potential, many of these peptides are derived from edible plant sources and can be delivered through functional foods or dietary supplements, offering a promising avenue for cancer prevention and adjunctive nutritional support. The review also touches upon the major hurdles in peptide drug development at present, such as stability, oral bioavailability, and large-scale production, while at the same time giving future perspectives that include bioengineering, nanotechnology-based delivery systems, and combination therapies for translating these natural products into clinical oncotherapeutics and health-promoting foods Full article

The forest within the Ben En National Park has a diverse flora, which, although protected, remains subject to degradation. The analysis and management strategies for forest degradation within this park were conducted using a phytogeographical approach supplemented by satellite imagery and a SWOT analysis. As a result, the area is characterized by nine distinct vegetation types comprising 1417 vascular plant species (from 902 genera and 196 families). These species belong to endemics from Northern, Central, and all of Vietnam, as well as 16 other phytogeographical elements. Tropical Asian and South China elements dominate the community structure in evergreen broad-leaved closed forests on both limestone and non-limestone mountains. Forest degradation is evident in changes to both floristic composition and vegetation structure. Floristic composition shows a trend of decreasing native elements while simultaneously increasing non-native or introduced elements. This “anthropogenic tropicalization” leads to a declining chain of ecological function from palaeotropical to introduced elements, resulting in biological invasion. For instance, the invasive species, Mimosa pigra, currently occupies about 442 ha in the semi-submerged zone of the Song Muc reservoir, indicating a loss of ecological function and a likely hydrological pathway for further spread. As a consequence of “anthropogenic tropicalization”, the vegetation is fragmented and gradually altered from a natural system to an anthropogenic one through a regressive succession from primary forest to bare land/invaded area. Based on the SWOT analysis, four management actions were proposed: 1—Establish a “sustainable native forest” program and “invasive species control” in the Song Muc reservoir; 2—Launch a “green livelihoods for the buffer zone” initiative; 3—Implement a “Smart forest monitoring” system; and 4—Forge an “ecotourism-conservation-community” alliance. Full article

Skin is a multifunctional organ essential for maintaining body homeostasis, regulating functions and providing protection from environmental stressors. In fish, skin is immune active, containing antimicrobial proteins acting as the first line of defence against infectious pathogens. The gills function similarly, as a key mucosal immunity site, where pathogens induce both innate and adaptive immune responses. In this study, proteomic analysis identified differentially expressed proteins in the skin and gills of ectoparasite Gyrodactylus turnbulli infected guppies (Poecilia reticulata) at two timepoints post-infection (Days 13 and 17). These fish were provided with a health-promoting additive, aimed to boost immunocompetency and reduce ectoparasite infections. Different proteomes were evidenced based on infection status of fish (susceptible, responding, or resistant) and in-feed supplementation. In skin tissue, susceptible fish showed no evidence of immune response, reflecting their high parasite load. Responding fish employed biological processes like apoptosis, reducing the gyrodactylid niche. In resistant fish, up-regulated innate and adaptive immunity explained the low parasite load on the host over the entire infection trajectory. Overall, fish protein expression in the skin and gills was affected both by the dietary supplement and gyrodactylid infection burden, highlighting the role of natural immunostimulants in aquatic infectious disease prophylaxis, control and treatment. Full article

Essential oils (EOs) are complex plant-derived mixtures increasingly investigated for their anti-inflammatory, antioxidant, and vasoprotective properties. Thromboinflammation, a process integrating coagulation, platelet activation, endothelial dysfunction, and inflammatory signaling, plays a central role in vascular pathology; however, the contribution of EOs to this process remains insufficiently characterized. This narrative review aims to synthesize current molecular and experimental evidence regarding the effects of EOs and their major bioactive constituents on pathways converging toward thromboinflammation. A focused PubMed/MEDLINE search, supplemented by manual reference screening, was conducted to identify experimental and translational studies on EOs and selected constituents relevant to inflammatory mediators, oxidative stress, endothelial dysfunction, platelet activation, and thrombotic pathways. Available data from predominantly preclinical experimental models indicate that EOs can exert multi-target effects, including modulation of cytokine production, attenuation of oxidative stress, improvement in endothelial function, and inhibition of platelet aggregation, thereby influencing key components of thromboinflammatory pathways. Despite these promising findings, heterogeneity in chemical composition, limited standardization, uncertain exposure relevance, and the predominance of preclinical data remain important limitations. In conclusion, EOs represent a promising but still largely preclinical class of natural compounds capable of modulating interconnected mechanisms relevant to thromboinflammation; however, further translational and clinical studies are required to validate their therapeutic potential. Full article

Gluten-free cereals and pseudocereals such as oats, quinoa, and buckwheat are widely used as bases for functional foods due to their protein quality, minerals, fiber, and polyphenols. However, they contain relatively low levels of some vitamins. This study evaluated the enrichment of cereal porridges with a cell-free homogenate of the carotenogenic yeast Rhodotorula toruloides (RT) as a natural strategy to enhance their nutritional value. Model porridges prepared from gluten-free oats, quinoa, buckwheat, and their blends were supplemented with 0%, 5%, or 10% R. toruloides homogenate (RTh). Samples were analyzed for antioxidant capacity (ABTS), lipid-soluble vitamins and provitamins (HPLC), fatty acid composition (GC-FID), approximate prebiotic potential, and cytotoxicity using the MTT assay on Caco-2 cells. The addition of RTh significantly increased antioxidant properties, with the highest value observed in buckwheat porridge with 5% RTh (1.9 mg TE/g DW). Lipid-soluble metabolites were detected only in enriched samples, reaching up to 420 µg/g DW ergosterol and 300 µg/g DW total carotenoids, mainly torularhodin. Quinoa porridges showed the highest PUFA content, whereas RTh was rich in oleic acid. Enrichment increased MUFA levels and improved the fatty acid profile. Approximate prebiotic potential was strongest in oat–quinoa blends. Cytotoxicity remained low, with most IC₅₀ values above 2000 µg/mL. Supplementation with 5% R. toruloides homogenate effectively improves antioxidant and lipid composition of gluten-free porridges while maintaining low cytotoxicity. Full article

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. Its main contributors are obesity, insulin resistance, diabetes and metabolic syndrome. Liver pathogenesis exacerbates when oxidative stress, inflammation, lipid accumulation, and attenuated autophagy signals coexist together with the main determinants of the liver disease. These findings may indicate that the suppression of the disease requires multi-targeting compounds to alleviate more than one factor, resulting in improved histopathological outcomes. This review studies natural compounds, given as supplements, with antioxidant and anti-inflammatory properties. The compounds included are vitamins, carotenoids, low-molecular-weight thiol-containing compounds, fatty acids and others that have been investigated for their pleiotropic activity alone or in combination. They act at different pathways and signals, and at gene expression control, modulating oxidative stress and inflammation, such as collagen, TNF-α, NF-κB, Nrf2 and PPARs genes. Their mechanism of action and characteristics may be encouraging treatment options as multi-targeting compounds for NAFLD and other diseases whose pathophysiology is closely related to metabolic syndrome. However, extensive study on their safety, toxicity, mechanisms of action and dosage regimen is needed before their final establishment as potential treatment options. Full article

Oxidative stress appears to be implicated in both the initiation and progression of autoimmune thyroiditis. Selenomethionine, which exhibits antioxidant properties, has been shown to reduce thyroid antibody titers in patients with autoimmune thyroiditis. Recent evidence suggests that vitamin E, a fat-soluble antioxidant, may protect against the development of autoimmune thyroiditis, and that its supplementation has been associated with improvements in female sexual function. The objective of the present pilot study was to determine whether vitamin E intake modulates the effects of selenomethionine on female sexual function and depressive symptoms in individuals with thyroid autoimmunity. The study enrolled three groups of reproductive-age women with euthyroid autoimmune thyroiditis, with 26 participants in each group. The groups were matched for age, thyroid peroxidase antibody titers, and TSH levels and differed according to vitamin E intake: adequate intake (group A), low intake (group B), and high intake (group C). All participants received selenomethionine supplementation (200 µg/day) for six months. Antibody titers and hormone levels were measured, and participants completed questionnaires assessing female sexual function (FSFI) and depressive symptoms (BDI-II). At baseline, no differences in biochemical outcomes were observed between the groups, except for testosterone levels. The study groups differed in sexual desire and arousal domain scores, which were higher in group A than in the other two groups. Total FSFI scores, the remaining FSFI domain scores, and BDI-II scores did not differ between groups at baseline. Across all groups, selenomethionine reduced thyroid peroxidase and thyroglobulin antibody titers and increased SPINA-GD and the ratio of free triiodothyronine to free thyroxine; however, the effects on antibody titers were most pronounced in group A. An increase in SPINA-GT and testosterone levels following selenomethionine supplementation was observed only in group A. In this group, selenomethionine also led to significant improvements in total FSFI scores and all individual domain scores. In contrast, in the remaining groups, the effects of supplementation were limited to increases in domain scores for lubrication, sexual satisfaction, and pain. A treatment-related reduction in total BDI-II scores was observed exclusively in women with adequate vitamin E intake. These findings suggest, for the first time, that dietary intake of a natural antioxidant may influence the effects of exogenous selenomethionine on sexual function and depressive symptoms in reproductive-age women with euthyroid autoimmune thyroiditis. Full article

Petroleum contamination of soils remains a significant environmental problem in boreal regions, where low temperatures constrain natural attenuation processes and complicate bioremediation. Nitrogen biostimulation is widely used to enhance petroleum hydrocarbon degradation; however, the combined effects of temperature regime, nitrogen form, contamination level, and nitrogen dosage remain insufficiently resolved for sandy podzolic soils of northern regions. This study investigated nitrogen-assisted biostimulation of petroleum-contaminated sandy podzolic soil collected in the Khanty–Mansi Autonomous Okrug (Western Siberia, Russia) using a factorial experimental design. Soil samples were artificially contaminated with crude oil at concentrations of 25, 50, and 100 g kg⁻¹ and incubated under warm and cold temperature regimes. Two nitrogen sources, urea and ammonium nitrate, were applied at several dosages. Changes in residual petroleum hydrocarbon content were monitored together with the abundance of culturable microorganisms under the applied cultivation conditions at the intermediate contamination level on day 60. Nitrogen supplementation enhanced petroleum hydrocarbon removal relative to the untreated control, but the magnitude of the effect depended substantially on temperature, nitrogen form, and contamination level. Under the tested conditions, ammonium nitrate was generally associated with stronger hydrocarbon removal than urea, particularly at the intermediate contamination level (50 g kg⁻¹). The results indicate that the response to nitrogen biostimulation in sandy boreal soils is controlled by interacting experimental factors rather than by nitrogen addition alone. These findings improve the positioning of nutrient-assisted remediation in cold-region soils and provide a basis for future mechanistic and field-scale studies. Full article

This study conducts a cradle-to-gate Environmental Life Cycle Assessment (E-LCA) of tea production in Sri Lanka, comparing smallholder and estate-owned plantations processed by Orthodox and Crush–Tear–Curl (CTC) methods. Unlike most tea LCA studies that treat cultivation as a single undifferentiated phase, this work explicitly incorporates the perennial nature of tea by using a modular life cycle framework that separates the agronomic stages alongside factory processing up to the packed-tea gate. This approach allows a more precise allocation of long-term environmental burdens over the entire productive lifespan of the tea plant, addressing a methodological gap in the literature. Four production scenarios were evaluated: Smallholder-Orthodox, Smallholder-CTC, Estate-Orthodox, and Estate-CTC, with the functional unit set to 1 tonne of processed tea. Primary data were gathered through structured surveys of 30 plantations (25 smallholders, 5 estates) and 5 tea factories, supplemented by secondary data from Ecoinvent v3.11 and national statistics. The CML-IA Baseline method in SimaPro v9.5 was applied to characterize impacts across eight impact categories: global warming potential (GWP), abiotic element depletion, fossil fuel depletion, acidification, human toxicity, terrestrial ecotoxicity, freshwater ecotoxicity, and eutrophication. Results indicate that Smallholder-Orthodox systems have the highest GWP (3304 kg CO₂ eq per tonne), whereas Estate-CTC systems show a lower GWP (2894.87 kg CO₂ eq). Acidification potential ranges from 47.21 kg SO₂ eq for Smallholder-Orthodox to 41.25 kg SO₂ eq for Estate-CTC. Overall, the findings suggest that the scale of plantation management has a greater impact on environmental performance than processing technology, highlighting the need to focus sustainable practices on the cultivation stage, exactly where the perennial crop modeling approach used here provides the greatest analytical benefit. Full article

Background/Objectives: Dietary antioxidants are frequently utilized by breast cancer (BC) patients to mitigate treatment-related toxicities and enhance quality of life. However, their clinical efficacy remains highly controversial due to conflicting epidemiological and clinical data. This review aims to critically evaluate the molecular mechanisms, clinical outcomes, and translational challenges of antioxidant supplementation in BC management. Methods: A comprehensive evaluation of current literature—encompassing observational cohorts, randomized controlled trials, and mechanistic in vitro/in vivo models—was conducted. The analysis focused on the pharmacological interactions of diverse bioactive compounds (polyphenols, vitamins, carotenoids) with BC progression and standard antineoplastic regimens. Results: Current evidence demonstrates a paradoxical, double-edged role of antioxidants in oncology. While specific interventions (e.g., Coenzyme Q10, melatonin) effectively ameliorate treatment-induced toxicities without compromising therapeutic efficacy, the concurrent administration of antioxidants during cytotoxic chemotherapy can inadvertently neutralize essential reactive oxygen species (ROS), correlating with increased disease recurrence and mortality. Furthermore, clinical translation is severely hindered by the intrinsic hydrophobicity of natural compounds, the lack of whole-food matrix standardization, and dose-dependent hepatotoxicity. Emerging targeted delivery systems, such as lipid nanoformulations, show significant potential in overcoming these pharmacokinetic barriers. Conclusions: The therapeutic viability of antioxidant supplementation in BC is not universal; it is heavily dictated by intrinsic tumor biology, specific treatment modalities, and chronopharmacology. These findings underscore a critical biological imperative to transition from generalized dietary guidelines toward a rigorous paradigm of precision nutritional oncology, strictly avoiding concurrent antioxidant supplementation during active oxidative therapies. Full article

The emergence of membrane boundaries represents a decisive transition in the origin of life, yet the molecular nature of the earliest abiotic membranes remains uncertain. Existing models based on simple fatty acids, while experimentally tractable, often lack the environmental robustness required under fluctuating prebiotic conditions. Furthermore, the absence of clear pathways linking primitive amphiphiles to later phospholipid systems highlights the need for chemically continuous intermediate frameworks. Here, we explore borate-bridged amphiphile–carbohydrate conjugates as plausible intermediates between simple prebiotic surfactants and modern lipid bilayers. These conjugates arise from low-molecular-weight polyols—including glycerol, butane-1,2,3,4-tetraol, pentane-1,2,3,4,5-pentaol, and hexane-1,2,3,4,5,6-hexitol—reacting with long-chain alkyl ethers and borate species under alkaline conditions, enabling reversible coupling to ribose and other vicinal diol-containing sugars. This chemistry integrates three essential properties for early compartmentalization: hydrolytically robust ether-linked hydrophobic domains, multivalent and highly hydrated headgroups, and environmentally responsive borate coordination. Comparative physicochemical analysis suggests that single-tail alkylglycerol derivatives preferentially form micelles and interfacial films, while di- and tri-tail tetritol and pentitol conjugates favor lamellar assemblies and vesicle formation across realistic prebiotic pH and salinity ranges. Hexitol-based systems, particularly those bearing three hydrophobic chains, may act as membrane-stabilizing components that enhance rigidity and reduce permeability under extreme conditions. We propose that heterogeneous mixtures dominated by two-tail polyol diethers, supplemented by tri-tail stabilizers and surface-active alkylglycerols, could provide mechanically robust, pH-tunable, and sugar-decorated abiotic membranes. Such borate-mediated amphiphiles offer a chemically coherent framework linking carbohydrate stabilization, ether lipid persistence, and dynamic self-assembly, potentially representing a transitional stage in the evolutionary pathway from primitive amphiphilic films to biologically encoded membranes. Full article