Search Results (8,651)

Soils act as sources or sinks for three major greenhouse gases (CO₂, CH₄, and N₂O). Approximately 20% of global CO₂ emissions are released from soils through the soil respiration process. Soil respiration (soil CO₂ emission) can account for over 85% of ecosystem respiration. The aim of this study was to compare managed and unmanaged stands of pedunculate oak (Quercus robur L.) in order to investigate the impact of forest management on soil CO₂ emissions. We selected one managed and two unmanaged stands. The first stand (S1) represents a managed middle-aged stand, which is the optimal stage of development. The second stand (S2) belongs to the over-mature stage of development in an old-growth oak forest, while the third stand (S3) belongs to the decay stage of development in an old-growth oak forest. The closed chambers method was used for air sampling and the air samples were analyzed using gas chromatography (GC). Multiple regression models that include soil temperature (ST), soil moisture (SM), and their interaction provide a better explanation for variation in soil CO₂ emission (SCDE) (higher R² values) compared to regression models that only involve two variables (ST and SM). The study showed that SCDE in the decay stage of old-growth forest (S3) was significantly lower (p < 0.001) compared to the other two stands (S1 and S2). S3 is characterized by very low canopy cover and intensive natural regeneration, unlike S1 and S2. However, there were no significant differences in SCDE between the managed middle-aged stand (S1) and the over-mature (old-growth) stand (S2). Over a long-term rotation period in pedunculate oak forests, forest management practices that involve the periodic implementation of moderate silvicultural interventions can be deemed acceptable in terms of maintaining the carbon balance in the soil. Full article

Background: This study investigated whether diaphragmatic breathing intervention could lead to acute improvements in trunk and shoulder mobility and pulmonary function in healthy young adults. Methods: Twenty-six physically active males (aged 24.3 ± 2.0 years, body height of 182.9 ± 6.4 cm, and body weight of 82.8 ± 10.4 kg) were randomly assigned to either an experimental or a control group. The experimental group underwent a 22 min diaphragmatic breathing intervention in a lying position. The control group lay passively, breathing naturally. Mobility assessments (chest expansion, thoracic spine rotation, lateral trunk flexion, and shoulder girdle mobility) and pulmonary function tests (forced vital capacity, forced expiratory volume in one second and their ratio) were conducted before and after the intervention. Results: Only experimental group showed significant improvements after the intervention (p ≤ 0.01) in the chest expansion (+22.2%, ES = 0.62), thoracic spine rotation (+21.7%, ES = 0.76 on the left and +23.3%, ES = 0.84 on the right side), lateral trunk flexion (+11.7%, ES = 0.62 on the left and +15.4%, ES = 1 on the right side), shoulder girdle mobility (+20.2%, ES = 0.44 on the left and +21.5%, ES = 0.38 on the right side), forced vital capacity (+4.7%, ES = 0.39) and reduction (p ≤ 0.01) in ratio between forced expiratory volume in one second and forced vital capacity (−4.6%, ES = 0.47). Conclusion: The results revealed that a 22 min diaphragmatic breathing intervention could immediately improve trunk and shoulder mobility and pulmonary function, likely due to anatomical relationships and more efficient use of respiratory muscles, especially the diaphragm. Full article

Cannabidiol (CBD), a non-psychoactive phytocannabinoid derived from Cannabis sativa L., has emerged as a promising multifunctional agent in dermatology and cosmetic science. The review provides an updated synthesis of CBD’s topical therapeutic potential, challenges, and evolving regulatory frameworks. CBD exhibits diverse biological effects, including anti-inflammatory, antioxidant, antibacterial, analgesic, lipostatic, antiproliferative, moisturising, and anti-ageing properties through interactions with the skin’s endocannabinoid system (ECS), modulating CB1, CB2, TRPV channels, and PPARs. Preclinical and clinical evidence support its efficacy in managing acne, psoriasis (including scalp psoriasis), atopic and seborrheic dermatitis, and allergic contact dermatitis. CBD also relieves pruritus through neuroimmune modulation and promotes wound healing in conditions such as pyoderma gangrenosum and epidermolysis bullosa. In hair disorders such as androgenetic alopecia, it aids follicular regeneration. CBD shows promise in managing skin cancers (melanoma, squamous cell carcinoma, Kaposi sarcoma) and pigmentation disorders such as melasma and vitiligo. It enhances skin rejuvenation by reducing oxidative stress and boosting collagen and hydration. However, there are challenges regarding CBD’s physicochemical stability, skin penetration, and regulatory standardisation. As consumer demand for natural, multifunctional skincare grows, further research is essential to validate its long-term safety, efficacy, and optimal formulation strategies. Full article

Rubella is typically a mild viral illness, but it can lead to severe complications when contracted during pregnancy, such as pregnancy loss or developmental defects in the fetus (congenital rubella syndrome). Therefore, it is crucial to develop and maintain protective immunity in women of childbearing age. In this study, we assessed the transcriptional factors associated with rubella-specific immune outcomes (IgG binding antibody and avidity, neutralizing antibody, and memory B cell ELISpot response) following a third MMR vaccine dose in women of reproductive age to identify key factors/signatures impacting the immune response. We identified baseline (Day 0) and differentially expressed (Day 28–Day 0) genes associated with several RV-specific immune outcomes, including the transferrin receptor 2 (TFR2), which is an important factor regulating iron homeostasis and macrophage functional activity, and a close functional homolog of TFR1, the cellular receptor of the New World hemorrhagic fever arenaviruses. We also identified enriched KEGG pathways, “cell adhesion molecules”, “antigen processing and presentation”, “natural killer cell-mediated cytotoxicity”, and “immune network for IgA production”, relevant to immune response priming and immune activation to be associated with RV-specific immune outcomes. This study provides novel insights into potential biomarkers of rubella-specific immunity in women of childbearing age. Full article

Calibrating the ages, masses, and radii of stars on the upper main sequence depends heavily on accurate measurements of the effective temperature ($T_{\mathrm{eff}}$) and surface gravity ($logg$). These parameters are difficult to obtain meticulously due to the nature of hot stars, which exhibit features such as rapid rotation, atomic diffusion, pulsation, and stellar winds. We compare the $T_{\mathrm{eff}}$ and $logg$ values of apparent normal B-F stars in four recent catalogues that employ different methods and pipelines to obtain these parameters. We derived various statistical parameters to compare the differences between the catalogues and discussed the astrophysical implications of these differences. Our results show that the huge differences in $T_{\mathrm{eff}}$ (up to ${10}^4$ K) and $logg$ (up to 2 dex) between the catalogues have serious implications on the determination of ages, masses, and radii of the stars in question. We conclude that there appears to be no homogeneous set of stellar parameters on the upper main sequence, and one must be cautious when interpreting results obtained from using only one of the catalogues. The homogenisation of said parameters is an essential task for the future and will have a significant impact on astrophysical research dealing with stars on the upper main sequence. Full article

The search for novel natural resources, such as extracts from algae and plant for use as reductants and capping agents for the synthesis of nanoparticles, may be appealing to medicine and nanotechnology. This study aimed to use Malva parviflora fruit extract as a novel source for the green synthesis of silver nanoparticles (AgNPs) and to evaluate their characterization. The results of biosynthesized AgNP characterization using multiple techniques, such as UV–Vis spectroscopy, scanning electron microscopy (SEM), FTIR analysis, and zeta potential (ZP), demonstrated that M. parviflora AgNPs exhibit a peak at 477 nm; possess needle-like and nanorod morphology with diameters ranging from 156.08 to 258.41 nm; contain –OH, C=O, C-C stretching from phenyl groups, and carbohydrates, pyranoid ring, and amide functional groups; and have a zeta potential of −21.2 mV. Moreover, the antibacterial activity of the M. parviflora AgNPs was assessed against two multidrug-resistant strains, including Staphylococcus aureus MRSA and Escherichia coli ESBL, with inhibition zones of 20.33 ± 0.88 mm and 13.33 ± 0.33 mm, respectively. The minimum bactericidal concentration (MBC) was 1.56 µg/mL for both. SEM revealed structural damage to the treated bacterial cells, and RAPD-PCR confirmed these genetic alterations. Additionally, M. parviflora AgNPs showed antioxidant activity (IC₅₀ = 0.68 mg/mL), 69% protein denaturation inhibition, and cytotoxic effects on MCF-7 breast cancer cells at concentrations above 100 µg/mL. These findings suggest that M. parviflora-based AgNPs are safe and effective for antimicrobial and biomedical applications, such as coatings for implanted medical devices, to prevent biofilm formation and facilitate drug delivery. Full article

Background/Objectives: Idiopathic pulmonary fibrosis (IPF) registries are established to enhance understanding of its natural history. Methods: Serbia (RS) participated in the EMPIRE (European Multi-Partner IPF Registry) from June 2015 to October 2022, involving four centers. The registry included patients over 18 diagnosed with IPF based on the 2011 international criteria. We aimed to gather key clinical, functional, and survival data, along with treatment information for IPF patients in RS, using a centralized electronic case report for consistency. Results: 188 RS patients participated (median age at diagnosis 65, 63.8% male, 51% smoking history, 56% radiological usual interstitial pneumonia (UIP) pattern). At the diagnosis, median forced vital capacity (FVC) was 73.7% and diffusion capacity for carbon monoxide (DL_CO) was 38%. At initiation of antifibrotic therapy, median FVC was 73.2% (71.5% for deceased, 75.8% for survivors (p = 0.455), and DL_CO was 33.8% (19.9% for deceased, and 35.6% for survivors (p = 0.046)). The median long-term survival from diagnosis was 29.4 months (95% CI: 22.6–36.2 months), and 9.4 months (95% CI: 5.9–12.9 months) from the initiation of therapy, with no difference in the duration of antifibrotic treatment between survivors and deceased (p = 0.598). Conclusions: The RS EMPIRE cohort represents a younger, less comorbid population with fewer smokers and more probable UIP, factors linked to a favorable prognosis. Nevertheless, survival was poorer than expected, mainly due to advanced disease severity at the time of antifibrotic initiation, as indicated by lower DL_CO. These findings highlight the importance of earlier diagnosis and treatment before significant physiological decline to improve outcomes. Full article

(1) Background: Violence against healthcare professionals is becoming a growing concern for healthcare systems and a public health issue, and in Portugal it remains undocumented at a national level, leaving a critical knowledge gap. This scenario compromises the development of effective public policies and evidence-based institutional strategies, which are essential for guiding policymakers in the implementation of preventive measures and appropriate safety protocols to assess the nature, frequency, and key factors contributing to violence against healthcare professionals (doctors and nurses) in clinical settings. (2) Methods: This is a quantitative, descriptive, and cross-sectional study. The sample size was 440 professionals (n = 440). Between January and May 2024, healthcare professionals (physicians and nurses) working in four local health units located in the south of Portugal were invited to participate in this study via institutional e-mail. Data was collected using a structured questionnaire on the healthcare professional’s sociodemographic and work-related characteristics and aspects related to violence towards healthcare professionals in the workplace. Unconditional logistic regression models were fitted to compute crude odds ratios (ORs) and 95% confidence intervals (95%CIs) for the association between sociodemographic and work-related characteristics and violence at work. (3) Results: Nearly 40% of the healthcare professionals sampled reported having been victims of violence in the workplace, and, among these, the majority reported experiencing psychological violence (94.2%), followed by physical violence (46.2%), another type of violence (39.1%), and sexual violence (4.1%). Incidents were mostly occasional (65.5%), occurring during the daytime (51.5%) and on weekdays (84.8%). Healthcare professionals aged between 34 and 55 years old were approximately twice as likely to experience violence compared to those who were 56 years old or older (OR = 2.28; 95%CI 1.33–3.90). Also, those who had been with the organization for more than 4 years (5–7 years: OR = 2.37; 95%CI 1.05–5.37. ≥8 years: OR = 1.87; 95%CI 1.00–3.50), as well as those who worked shifts (OR = 1.84; 95%CI 1.25–2.72), reported incidents of violence more frequently. (4) Conclusions: The low response rate (12.5%) and cross-sectional design limit the generalizability of the results, which should be interpreted considering these methodological limitations. Workplace violence in Portugal is a reality, and it requires solutions. Information related to violent incidents must be comprehensively gathered to understand the full extent of the problem and develop prevention strategies based on potentially changeable risk factors to minimize the negative effects of workplace violence. Full article

Background/Objectives: Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive fibrosing interstitial disease that incurs significant healthcare costs due to diagnostic and treatment needs. This study aimed to estimate healthcare expenses related to IPF diagnosis, treatment, and follow-up, including factors affecting overall expenditure. Methods: This retrospective cohort study included 276 IPF patients from a tertiary hospital (2013–2022). Diagnostic and treatment costs were analyzed, including antifibrotic medications (pirfenidone and nintedanib), diagnostic tests (pulmonary function tests and performance evaluation tests), and interventions (fiberoptic bronchoscopy, imaging modalities). Costs in Turkish Lira were converted to United States dollars. Statistical analysis was performed using non-parametric tests to evaluate expenditure correlations with demographic, clinical, and treatment parameters, which included the Mann–Whitney and Spearman Rank Correlation tests when appropriate. Results: The median healthcare expenditure was USD 429.1 (9.13–21,024.57). Inpatient costs (USD 582.67; USD 250.22 to USD 1751, 25th and 75th percentile, respectively) were higher than outpatient costs (USD 192.36; USD 85.75 to USD 407.47, 25th and 75th percentile, respectively). Antifibrotic regimens did not differ significantly in cost or duration (Z = 0.657; p = 0.511) (mean pirfenidone duration: 1.1 ± 1.0 years; mean nintedanib duration: 0.6 ± 0.9 years). Diagnostic tests, particularly pulmonary function tests (PFT) (p: 0.001, Rho: 0.337), diffusing capacity of the lungs for carbon monoxide (DLCO) (p: 0.001, Rho: 0.516), and high-resolution computed tomography (HRCT) (p: 0.001, Rho: 0.327), were the primary drivers of costs. Longer treatment duration was positively correlated with expenditure (Rho: 0.264, p: 0.001 and Rho: 0.247, p: 0.006 for pirfenidone and nintedanib, respectively) while age showed a weak negative correlation (Rho = −0.184, p = 0.002). Gender and type of antifibrotic regimen did not show any significant effect on costs. Discussion: Diagnostic and follow-up testing were the main contributors to costs, driven by reimbursement requirements and the progressive nature of IPF. Antifibrotic medications, although expensive, provided clinical stability, potentially reducing hospitalization needs but increasing long-term care expenses. Variations in healthcare systems affect expenditures, with Turkey’s universal coverage lowering costs compared to Western countries. The study’s main limitations include being a single-center, retrospective study and its inability to include comorbidities and disease severity in the statistical analysis. Conclusions: IPF management is resource-intensive, with diagnostic tests and follow-up driving costs independent of demographics and treatment modality. Anticipating higher expenditures with prolonged survival and evolving treatment options is crucial for healthcare budget planning. Preparation of healthcare policies accordingly to these observations, which must include an overall increase in cost due to treatment duration and survival, remains a crucial aspect of budget control. Full article

Toxicological effects of microplastics (MPs) have been confirmed in a variety of microorganisms in aquatic environments, and they are closely correlated with the physicochemical properties of the MPs. In a natural environment, different aging treatments always induce different alterations in the physicochemical properties of MPs, thus influencing their environmental behaviors and biotoxicity. In this work, physicochemical properties and toxicity towards Escherichia coli (E. coli) were investigated in polystyrene (PS) MPs (3 and 10 μm) before and after aging by UV irradiation and biofilm formation, respectively. The results show that UV irradiation and biofilm formation led to different alterations in the surface morphologies and functional groups of PS. The UV-aged 3 μm PS had the strongest inhibitory effect on E. coli growth, and the bio-aged 10 μm PS had the strongest beneficial effect on E. coli growth. Also, the ATPase activity, production of intercellular ROS, and MDA content of the E. coli were affected differently. UV aging enhanced the toxicity of PS towards E. coli, while bio-aging had an opposite weakening effect. Overall, our research verified the remarkable differences in the physicochemical properties and biotoxicity of PS induced by UV aging and bio-aging. Full article

With the advancement of artificial intelligence, Agricultural Expert Systems (AESs) show great potential in enhancing agricultural management efficiency and resource utilization. Accurate extraction of semantic features from agricultural short texts is fundamental to enabling key functions such as intelligent question answering, semantic retrieval, and decision support. However, existing single-structure deep neural networks struggle to capture the hierarchical linguistic patterns and contextual dependencies inherent in domain-specific texts. To address this limitation, we propose a hybrid deep learning model—Bidirectional Encoder Recurrent Neural Network (BERNN)—which combines a domain-specific pre-trained Transformer encoder (AgQsBERT) with a Bidirectional Long Short-Term Memory (BiLSTM) network. AgQsBERT generates contextualized word embeddings by leveraging domain-specific pretraining, effectively capturing the semantics of agricultural terminology. These embeddings are then passed to the BiLSTM, which models sequential dependencies in both directions, enhancing the model’s understanding of contextual flow and word disambiguation. Importantly, the bidirectional nature of the BiLSTM introduces a form of architectural symmetry, allowing the model to process input in both forward and backward directions. This symmetric design enables balanced context modeling, which improves the understanding of fragmented and ambiguous phrases frequently encountered in agricultural texts. The synergy between semantic abstraction from AgQsBERT and symmetric contextual modeling from BiLSTM significantly enhances the expressiveness and generalizability of the model. Evaluated on a self-constructed agricultural question dataset with 110,647 annotated samples, BERNN achieved a classification accuracy of 97.19%, surpassing the baseline by 3.2%. Cross-domain validation on the Tsinghua News dataset further demonstrates its robust generalization capability. This architecture provides a powerful foundation for intelligent agricultural question-answering systems, semantic retrieval, and decision support within smart agriculture applications. Full article

Artificial Intelligence (AI) profoundly shapes adolescents’ digital experiences, presenting both developmental opportunities and risks related to privacy and psychological well-being. This study investigates first the possible generational gap between adolescents and their parents in AI use and trust, and then the associations between the Trait Emotional Intelligence (trait EI), parenting styles, perceived social support, and parental involvement on adolescents’ use and trust in AI-based technologies. Participants were 170 adolescents (aged 13–17) and 175 parents from southern Italy, who completed standardized questionnaires assessing parenting styles, Trait Emotional Intelligence (Trait EI), social support, digital literacy, and use and trust in AI. Adolescents used AI more frequently than parents, especially for school- or work-related support and were more likely to seek behavioral advice from AI. They also showed higher trust in AI data security and the quality of behavioral advice than parents. Moreover, greater trait EI and more authoritative (vs. authoritarian) parenting were associated with less frequent AI use and lower use and trust in AI. In 47 matched parent–adolescent dyads, cluster analysis identified Balanced Users (higher trait EI, authoritative parenting, stronger support, cautious AI use) and At-Risk Users (lower trait EI, authoritarian parenting, lower support, heavier and more trusting AI use) Despite no causal inferences can be drawn due to the correlational nature of the data, the results suggested the importance of considering adolescents’ trait EI and authoritative parenting practices in supporting balanced and critical digital engagement, highlighting the concept of a “digital secure base” as essential for navigating the evolving digital landscape. Full article

Background/Objectives: Femoral neck fractures are a growing concern due to their increasing incidence in both elderly and younger populations. Preoperative CT scan evaluation is often used for better understanding of fracture patterns of femoral neck fractures that need to be treated using cannulated screws. Methods: The present study retrospectively analyzed 55 patients treated with cannulated screw fixation over seven years. Preoperative CT scans, fracture classification (Garden and Pauwels), and surgical timing were evaluated. Results: All 55 patients were treated with three cannulated screws by nine fellowship-trained surgeons. The average age of the CT-yes group was 54.44 years (SD 15.45), while the average age of the CT-no group was 56.93 (SD 14.95). Differences in age among the two groups were not statistically significant. In 4 patients, the treatment with cannulated screws failed, leading to a subsequent total hip arthroplasty for avascular necrosis (AVN). Three of them belonged to the CT-yes group, while only one belonged to the CT-no group. A statistical analysis showed no significant differences between patients who underwent a CT scan and those who did not have a CT scan and their results (p = 0.282).Results indicate no statistically significant difference in outcomes between patients who underwent a CT scan before the surgical treatment and those who did not, while a well-conducted X-ray assessment is essential and can be sufficient to fully understand and treat most of the fractures. Conclusions: The risks of avascular necrosis and non-union must be considered in the decision-making process regarding the suitable treatment. Early surgery did not significantly improve treatment results, but it is recommended. Fixation with cannulated screws remains a good treatment, especially for some patterns of fractures and younger patients. Given the study’s limitations, including the small sample size and retrospective nature, prospective multicenter studies are warranted to better understand the role of CT scans in optimizing surgical planning and improving patient outcomes. Full article

It is widely accepted that chronic inflammation constitutes a significant mechanism that promotes the biological aging process. The pineal gland is regarded as being closely related to the control of the “life clock”. The present study aimed to determine the inflammation associated with pinealectomy in the rat hippocampus and to investigate the extent to which age stage impacts the severity of this inflammation. We evaluated the expression of the Akt/NF-kB signaling pathway in neurons and gliosis level in the dorsal hippocampus (dHipp) of rats subjected to sham surgery or pinealectomy at 3, 14, or 18 months of age. The assessment was conducted using immunohistochemistry. Removal of the pineal gland resulted in significant, region-specific increases in NF-kB expression in neurons of the dHipp in the youngest and middle-aged groups. However, the change in expression of the phosphorylated form of Akt (pAkt1) in neurons went in the opposite direction in these two age groups, and there were also regional differences. Pinealectomy triggered microgliosis in both young and old rats, but middle-aged rats were resistant to microglia activation. Conversely, astrogliosis was observed in young adult and middle-aged groups with melatonin deficiency in certain regions of the dHipp. It is noteworthy that young adult rats demonstrated the highest degree of vulnerability to inflammation associated with the loss of melatonin as a hormone. In contrast, middle-aged rats with pinealectomy exhibited a complex and partial adaptive response. These findings emphasize the dynamic and age-dependent nature of neuroinflammation following pinealectomy, underscoring the developmental stage as a critical determinant of inflammatory susceptibility. Full article

Background/Objectives: The transcription factor 7-like 2 (TCF7L2) rs7903146 polymorphism has been strongly associated with type 2 diabetes mellitus (T2DM) in various populations; however, its impact on different ethnic groups is not fully understood. Given the distinct minor allele frequency in Chinese populations, this study aimed to analyze the association of rs7903146 with the risk of T2DM in a Han Chinese cohort and its relationship with relevant clinical parameters. Methods: We conducted a case–control study including 600 patients with type 2 diabetes mellitus (T2DM) and 511 sex-matched non-diabetic controls of Han Chinese descent. The TCF7L2 rs7903146 (C/T) polymorphism was genotyped using a TaqMan™ SNP assay. Clinical parameters, including body mass index (BMI), fasting plasma glucose, hemoglobin A1c, lipid profile, and high-sensitivity C-reactive protein (hs-CRP), were compared between genotypes. Logistic regression analyses were performed under a dominant genetic model (CT/TT vs. CC), adjusting for age, sex, systolic and diastolic blood pressure, BMI, and smoking status. Subgroup analyses were conducted by sex, BMI category, age at diagnosis, and family history of T2DM. Given the exploratory nature of this study and the low frequency of the TT genotype, no formal correction for multiple testing was applied. Results: Frequencies of the CT and TT genotypes were higher in the diabetic group (p = 0.045) and were significantly associated with an increased risk of T2DM under a dominant genetic model (adjusted OR = 2.24, p = 0.025). Individuals with CT/TT genotypes had elevated fasting glucose and hs-CRP levels; these genotypes were also linked to higher BMI in the female T2DM patients. The T allele frequency varied across ethnic groups, being lowest in East Asians and highest in Latin (Brazilian/mixed ancestry) populations. Mechanistically, the T allele may contribute to T2DM via altered TCF7L2 expression, impaired insulin secretion, inflammation, and metabolic dysregulation. Conclusions: The TCF7L2 rs7903146 T allele was associated with an increased risk of T2DM and higher fasting glucose and hs-CRP levels in this Han Chinese cohort. The CT/TT genotypes were also associated with higher BMI in the female T2DM patients. While the findings are consistent with the known effects of this variant in other populations, mechanistic hypotheses such as the involvement of inflammatory or metabolic pathways remain hypothetical and warrant further functional validation. Full article