Search Results (144)

Modern agriculture is undergoing a paradigm shift toward eco-friendly methodologies that enhance seed material quality while minimizing chemical inputs. This study evaluates the impact of Effective Microorganism (EM) exposure (variants E1 and E2) on the morpho-physiological parameters and phytosanitary health of potato tubers. The primary objective was to determine the efficacy of microbial priming in suppressing the infection rates of Streptomyces scabies (common scab) and Rhizoctonia solani (black scurf) across 14 genetically diverse cultivars. A three-year field experiment (2019–2021) was conducted using a split-plot design with three replications. The study analyzed the interaction between EM exposure times and the genetic resistance potential of the selected cultivars. Statistical analysis confirmed that pre-planting microbial treatments significantly inhibited pathogen development. EM applications (E1 and E2) reduced the infection rates of both S. scabies and R. solani through an “escape mechanism,” whereby treated tubers exhibited accelerated biomass accumulation and reached physiological maturity before peak pathogen pressure. Furthermore, treatments optimized the physiological state and vigor of the tubers, establishing a robust physiological barrier against soil-borne infections. The application of EMs proves to be a highly effective, non-invasive biostimulation method. A significant difference was observed in the responding varieties between EM treatments and the cultivars innate genetic resistance, particularly in cultivars with higher baseline resistance. The use of EM biostimulants significantly modifies the health of tubers, and the direction of these changes is strictly determined by the variety factors. The results suggest that microbial priming not only enhances plant growth kinetics but also induces systemic resistance, offering a viable ecological alternative to traditional chemical seed dressings in sustainable potato production. Full article

Management practices that optimize physiological responses of crops can be applied in agriculture to achieve higher productivity in challenging environments limited by nutrient availability. Extremely Low Frequency (ELF), a type of non-ionizing radiation in the range of 0.3 to 300 Hz, interacts with biological systems and has potential applications in sustainable agriculture. This study evaluates the effects of ELF on morphophysiological parameters of soybean plants during the vegetative stage. Plants grown under controlled conditions were subjected to ELF treatments—Control, TA (which increases interatomic spacing), and TB (which decreases interatomic spacing)—in combination with three nutrient solution strengths (50%, 75%, and 100% of the Hoagland solution). Chlorophyll index, root and shoot length, and dry mass were measured at the end of experiment. ELF treatment significantly enhanced chlorophyll index, with treatment TB showing the greatest increase. This may suggest improved nutrient assimilation of key nutrients such as nitrogen and magnesium, which are critical for chlorophyll synthesis. These findings demonstrate the potential of ELF treatment to enhance plant physiological performance, even under nutrient-limited conditions. When combined with nutrient solutions, ELF exposure may promote plant health and growth by increasing chlorophyll index and may improve nutrient uptake. This approach represents a promising and sustainable strategy to boost crop productivity and resource use efficiency in agricultural systems. Full article

The light spectrum and vapor pressure deficit (VPD) are key environmental factors that significantly influence the morphophysiological development and survival of micropropagated woody plants during acclimatization. However, few studies have focused on their interactive effects under ex vitro conditions. This study examined the combined effects of four light spectra (white, blue, red, and red–blue) and two VPD levels (low: 0.2 kPa; high: 1.0 kPa) on growth, photosynthesis pigments, biochemical indices, and leaf temperature of Pistacia spp. ‘UCB1’ plantlets over a 30-day acclimatization period. The results demonstrated that red–blue light under low VPD significantly enhanced plantlet performance across multiple parameters, resulting in the highest leaflet number (79.25 pieces), stem diameter (2.13 mm), leaf dry weight (0.048 g), leaf fresh weight (0.15 g), root length (1.48 cm), and leaf area (103.3 cm²). Furthermore, this treatment markedly increased anthocyanin, total soluble carbohydrate content, and photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoids). Principal component and correlation analyses identified that red–blue light under low VPD was strongly associated with traits linked to growth and photosynthetic ability, whereas blue and white light under high VPD showed the weakest responses. Entropy-weighted TOPSIS ranked red–blue light under low VPD as the most effective treatment for balanced morpho-physiological functions during acclimatization. These findings highlight the importance of optimizing spectral quality and VPD to enhance autotrophic transition and ex vitro establishment in pistachio plantlets. These findings are important for improving ex vitro survival and large-scale propagation efficiency of micropropagated pistachio plantlets. Full article

Erythrocyte aging is a fundamental physiological phenomenon that involves significant structural and nanomechanical alterations of the cells’ structure and function. Coupling optical, fluorescence, and Atomic Force Microscopy (AFM), we analyzed morphology, membrane roughness and nanomechanical properties of the very same RBCs arising from favism subjects, measured at different stages of their aging in vitro. We also investigated the evolution and abundance of vesicles arising from the cells over their senescence pathway. This approach combines high-resolution fluorescence imaging with the correlation of membrane topology and biomechanics. This explores the differences between investigation based on statistical morphometric parameters, such as membrane roughness, and those based on the measure of point-dependent nanomechanical properties. Our ultra-morphological study evidences the existence of clear differences in the aging of normal and favism erythrocytes that results in a larger number of cells with abnormal shapes and in a hyper-production of vesicles along the senescence pathway of favism cells. In explaining these differences, we focused on the roles played by the hemoglobin evolution and by the morpho-mechanical properties that are responsible for the skeletal alterations. In particular, our data reported evidence that the two corresponding degradative pathways are coupled and play an important enhancement role in promoting the progression of cell senescence. Full article

Heat and high-irradiance stress increasingly threaten almond production in Mediterranean environments, where rising temperatures and prolonged summer droughts impair photosynthetic performance and yield. This study evaluated the effectiveness of three mineral-based shielding materials: kaolin, basalt powder, and zeolite. We hypothesized that the foliar application of reflective mineral materials would reduce leaf temperature, enhance photosynthetic efficiency, and improve yield without altering nut nutraceutical quality. A two-year field experiment (2024–2025) was conducted using a randomized block design with four materials (untreated control, kaolin, basalt powder, and zeolite). Physiological traits (gas exchange, chlorophyll fluorescence, leaf temperature, and SPAD index), morpho-biometric and biochemical parameters, and yield components were assessed. Kaolin and basalt powder significantly lowered leaf temperature (−1.6 to −1.8 °C), increased stomatal conductance and net photosynthesis, and improved photochemical efficiency (Fv′/Fm′) and electron transport rates. These treatments also enhanced drupe weight, kernel dry matter, and productive yield (up to +32% compared with the control). Zeolite produced positive but less prominent effects. No significant differences were detected in fatty acid profile, total polyphenols, or antioxidant capacity, indicating that the materials did not affect almond nutraceutical quality. Principal component analysis confirmed the strong association between kaolin and basalt powder and improved eco-physiological performance. Overall, mineral shielding materials, particularly kaolin and basalt powder, represent a promising, sustainable strategy for enhancing almond orchard resilience under Mediterranean climate change scenarios. Full article

Creating an optimal light environment for different crops is crucial for achieving high yields under controlled environment agriculture conditions. Currently, there are no optimal technologies, including lighting technologies, for growing root crops (in particular radish) in CEA (Controlled Environment Agriculture). This study examined the effects of HPS (High-pressure sodium vapor lamps) and three original sunlike full-spectrum LED lamps on the morphophysiological characteristics and the diffuse reflectance indices of the leaves of two contrast radish cultivars. It was found that a higher blue light content (24%) in the spectrum of the LED 3 lamp contributed to the formation of radish plants with a more compact leaf rosette and maximum yield of roots (up to 19%) compared to the other two types of LED lamps. When treated with LED 3, photosynthesis efficiency was probably higher compared to LED 1 and LED 2, which led to a significant decrease in reflected radiation, especially in the blue and red ranges (by 5–143% and 32–86%, respectively). It was found that the genotype had a significant effect on all morphophysiological parameters of radish, while lighting treatment only affected the integral parameters (Pr—proportion of root crop, and Ai—attraction index) and leaf thickness. However, lighting treatment exhibited a greater impact on leaf reflection indices compared to the genotype, especially those related to chlorophyll content. The results of the study indicate that LED 3 lamps, simulating natural light at midday, are suitable for the production of radish root crops under CEA conditions. Full article

In Romania’s wild flora, several Iris species exhibit important ornamental characteristics, such as early spring flowering and resilience to abiotic stress. This study assessed the behavior to new ecological conditions, the ornamental potential, and the antioxidant capacity of the wild species of Iris brandzae using morpho-anatomical, physiological, and biochemical biomarkers. The study of phenotypic characteristics (number and size of leaves on sterile and fertile shoots, size of flowering stems, bracts protecting the flowers, and perianth-segments) aimed to confirm and supplement existing information in the literature, as well as to evaluate the ornamental potential of this species. Morphological analyses revealed clear differences between fertile and sterile shoots, while photosynthetic activity across phenophases showed values within normal parameters, with the maximum recorded during flowering and with the chlorophyll a/chlorophyll b ratio maintained at values close to 3:1, indicating favorable cultivation conditions. Biochemical investigations (total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity) demonstrated that dried plant material, particularly roots, contained higher levels of phenolic and flavonoid compounds and exhibited stronger antioxidant activity compared to fresh material. By integrating morpho-anatomical, physiological, and biochemical data, this research provides the first comprehensive characterization of I. brandzae beyond taxonomic and ecological descriptions. Our findings emphasize the species behavior under cultivation conditions, its ornamental value, and its potential as a source of bioactive compounds relevant to pharmaceutical applications. Full article

Soil acidity severely constrains coffee production by reducing nutrient availability and promoting aluminum toxicity and oxidative stress. Foliar zinc oxide nanoparticles (ZnO NPs) have been proposed as redox modulators that can improve nutrient homeostasis under abiotic stress. However, the safe and effective range of Coffea arabica L. remains unclear. In this study, seedlings were grown in acidic soil and sprayed twice with ZnO NPs at 10, 25, 50, and 100 mg L⁻¹. Morphophysiological, biochemical, and ionomic parameters were evaluated fifty days after treatment. Moderate ZnO NPs doses led to intermediate stomatal conductance values, whereas net photosynthesis showed intermediate but non-significant responses only at 10–25 mg L⁻¹, with higher doses (50–100 mg L⁻¹) causing a marked decline. These doses did not significantly modify hydrogen peroxide (H₂O₂) or malondialdehyde (MDA) levels in leaves or roots. In contrast, the highest dose (100 mg L⁻¹) induced a marked increase in H₂O₂ without affecting MDA, indicating a partial oxidative response rather than clear lipid peroxidation. Foliar analysis showed that 50 mg L⁻¹ ZnO NPs significantly increased P compared with the optimal soil, while Ca and K remained statistically similar across treatments. Na in the optimal soil was comparable to the 10–25 mg L⁻¹ ZnO NPs treatments, whereas Na at 50–100 mg L⁻¹ ZnO NPs was significantly reduced and foliar Zn increased markedly with increasing nanoparticle dose. Proline accumulation reflected a dose-dependent osmotic adjustment, and chlorophyll ratios indicated adaptive photoprotection. Overall, foliar ZnO NPs mitigated acidity-induced stress through physiological and ionomic adjustment, with 10–25 mg L⁻¹ identified as the physiologically safe range for C. arabica seedlings grown under acidic conditions. Full article

Lilium is one of the most valuable cut flowers in the world. Today, due to the global water crisis, the agricultural challenge is to modify cultivation patterns to reduce water requirements by plants. This research was conducted to optimize soilless cultivation systems and nutritional demands to improve morpho-physiological parameters of Oriental × Trumpet (Orienpet; OT) hybrid lily. Four soilless cultivation systems (aeroponic, ultrasonic, hydroponic in pots, and hydroponic in containers) were evaluated using three nutrient solutions (NS1, NS2, NS3), considering two variables: the ratio of ammonium to total nitrogen (0.1, 0.15, and 0.2) and the ratio of calcium to total cations (0.3, 0.5, and 0.7). A factorial experiment was used, arranged as a randomized complete block design. Plants grown in the hydroponic (pot) system treated with nutrient solution NS1 showed favorable and consistent results. Additionally, results revealed that the amount of malondialdehyde and, accordingly, the aging process were lower in the bulbs treated by NS3. Therefore, in addition to NS1, NS3 is also recommended for lily cultivation in the hydroponic (pot) system. Considering that photosynthetic performance improved with increasing ammonium in the nutrient solution of the ultrasonic system, it seems that the lily is an ammonium-feeding plant. However, this issue requires further evaluation. Full article

We evaluated the effects of suspensions of magnetite–maghemite nanocomposites functionalized with quaternized chitosan and phosphate groups on morpho-anatomical and physiological traits of yellow maize (Zea mays) and chili pepper (Capsicum annuum) seeds. A phytotoxicity assay was first conducted by applying aqueous suspensions of these nanocomposites to maize seeds at iron concentrations up to 100 ppm, using deionized water as the control under humid chamber conditions. After incubation, seeds treated with concentrations above 100 ppm exhibited reductions in biomass and root length compared with the control, suggesting phytotoxicity at high levels. Based on these results, suspensions containing 25, 35, and 45 ppm of iron, with corresponding phosphorus concentrations of 2.9, 4.0, and 5.2 ppm, were selected for a second in vitro assay using both maize and chili seeds. No statistically significant differences between treatments and control were detected for the variables measured. Germinated seeds from this assay were further evaluated under greenhouse conditions, where measured parameters also showed no significant differences between treatments and control in either crop. Overall, the findings indicate that aqueous suspensions of magnetite–maghemite nanocomposites with iron concentrations below 100 ppm do not produce phytotoxic effects on seed germination or morpho-anatomical and physiological traits measured. Full article

Cacti of the genera Opuntia and Nopalea exhibit morphophysiological and biochemical characteristics that favor their adaptation to semiarid environments, such as crassulacean acid metabolism (CAM) and cladode succulence. These strategies reduce water loss and allow the maintenance of photosynthesis under stress conditions. In this study, we evaluated the seasonal variation in the physiological and photochemical responses of forage cactus clones grown in semiarid environments, considering the rainy, dry, and transition seasons. The net photosynthetic rate (P_n) and chlorophyll fluorescence parameters varied significantly as a function of water availability and microclimatic conditions. We found higher CO₂ assimilation rates during the rainy season, while the dry season resulted in a strong impairment of photosynthetic activity, with reductions of 65% in stomatal conductance, 37% in transpiration, 20% in maximum quantum efficiency of photosystem II, and 19% in the electron transport rate. Furthermore, during these periods, we observed an increase in initial fluorescence and non-photochemical dissipation, demonstrating the activation of photoprotective mechanisms against excess light energy. During the transition seasons, the cacti exhibited rapid adjustments in gas exchange and energy dissipation, indicating the adaptive plasticity of CAM pathway. The MIU (Nopalea cochenillifera (L.) Salm-Dyck), OEM (Opuntia stricta (Haw.) Haw.), and IPA (Nopalea cochenillifera (L.) Salm-Dyck) clones demonstrated greater resilience, maintaining greater stability in P_n, instantaneous water use efficiency, and photochemical parameters during the drought. In contrast, the OEA (Opuntia undulata Griffiths) clone showed high sensitivity to water and heat stress, with marked reductions in physiological and photochemical performance. In summary, the photosynthetic efficiency and chlorophyll fluorescence of CAM plants result from the interaction between water availability, air temperature, radiation, and genotypic traits. This study provides a new scientific basis for exploring the effects of environmental conditions on the carbon and biochemical metabolism of cacti grown in a semiarid environment. Full article

The expected population rise will require a maximum exploitation of agricultural lands with a consequent increase in the demand for freshwater for irrigation uses. Future trends predict increasing periods of drought stress, which may impact on crop performance and limit the future production. Pepper is one of the most economically important crops and globally consumed vegetables. This crop is highly demanding in terms of water supply, and so far, developing tolerant cultivars is one of the main targets for breeding. The aim of this study is to accurately determine how pepper plants react to water stress at the vegetative stage in order to select genotypes that better cope with drought. We implemented the PhenoHort Plant Eye phenotyping platform to precisely assess changes in plant architecture and morpho-physiological parameters on 25 cultivated pepper genotypes (Capsicum annuum) under drought stress conditions. Three different irrigation supply levels were considered, including the control, intense, and severe water stress, by irrigating every 24, 72, and 96 h, respectively. Daily monitoring of 20 traits allowed ~190,000 multispectral and tridimensional data points through scans over 6 weeks of cultivation, thus shedding light on changes in plant architecture and vegetation indices’ values during stress. The dissection of genotype (G) and treatment (T) interactions revealed that digital biomass and plant height traits were strongly affected by the T factor (more than 50% of total variance), whereas color and multispectral parameters were under greater genotypic control, accounting for 58.27% and 64.97% of the total variance for HUE and NPCI, respectively. The comparison of each accession with respect to the control and the application of multivariate models allowed us to select four drought-tolerant lines (G1, G2, G22, and G25) able to reduce the effects of drought on the morphological parameters and architecture of the plant with positive effects on vegetative indices. This work represents the first attempt to dissect the response of pepper under drought stress at the vegetative stage using a high-throughput and non-invasive phenotyping system, offering new insights for selecting resilient genotypes. Full article

Background: Body surface area is a key determinant of cardiac morphology and function, but it is often underestimated in the interpretation of athlete’s cardiac phenotypes. Aims: This study aimed to assess the role of anthropometric characteristics and whether particularly high vs. low body surface area (BSA) is associated with distinct morpho-functional and cardiometabolic features in elite athletes. Methods: We retrospectively included 2518 Olympic athletes. All underwent a pre-participation screening, including physical examination, ECG, blood analysis, echocardiography, and cardiopulmonary exercise testing. Participants were grouped by sex-specific BSA percentiles: Group A (<5th percentile), Group B (25th–75th), and Group C (>95th percentile). Functional, echocardiographic, and cardio-metabolic parameters were compared among groups. Results: In male athletes, Group C showed higher resting systolic blood pressure (123.8 ± 10.4 mmHg) than Group B (117.4 ± 9.6, p < 0.0001) and Group A (110.4 ± 13, p < 0.0001), and a higher prevalence of dyslipidemia (31.7% vs. 11.1% in Group B and 4% in Group A, p = 0.031). Despite greater LVEDD (59 ± 3 mm in Group C vs. 55 ± 2.9 in B and 51.1 ± 3.1 in A, p < 0.0001) and LV mass (p < 0.0001), functional performance was lower in Group C, with VO₂ max/kg of 35.2 ± 13.2 mL/min/kg vs. 44 ± 7.1 in B, and 47.8 ± 7.3 in A (p < 0.0001). Similar trends were observed in females for morpho-functional parameters, though lipid profiles did not significantly differ among groups (p > 0.05). Conclusions: Anthropometric traits significantly influence the cardiovascular and metabolic phenotype of elite athletes. Our findings support the integration of anthropometric profiling into the routine cardiovascular assessment of athletes, especially those at the extremes of body size, to better interpret physiological adaptations and risk profiles. Full article

Salinity is a major abiotic stress that limits plant growth and survival. Colobanthus quitensis, the only native dicotyledon in the Antarctic Peninsula and southern South America, naturally inhabits environments with contrasting salinity regimes. This study compared the salt stress responses of three geographically distinct populations—Antarctic (pA), Magellanic coastal (pPA), and Andean inland (pC)—exposed to 0, 50, and 150 mM NaCl under controlled conditions. Morpho-physiological traits, photosynthetic parameters, osmolyte accumulation, oxidative damage markers, and antioxidant responses were evaluated. Population-specific strategies were observed. In pA, salinity reduced shoot biomass by 58% and doubled lipid peroxidation levels at 50 mM, indicating high oxidative stress. In pPA, shoot growth was maintained even at 150 mM, although chlorophyll and carotenoid contents decreased by approximately 20%, along with a reduction in total antioxidant capacity. In contrast, pC showed a coordinated tolerance response, maintaining biomass while accumulating the highest proline levels (742 µmol g⁻¹ FW at 150 mM) and enhancing total antioxidant capacity by 35% compared to the control. Multivariate analyses supported the contrasting strategies among populations. These results provide novel evidence of local adaptation and ecological plasticity in C. quitensis, particularly highlighting the hidden resilience of non-coastal populations. The findings support the potential of this extremophile species as a model system for investigating salinity tolerance and as a promising genetic resource for developing biotechnological strategies aimed at improving crop resilience under saline conditions. Full article

The aim of the study was to compare grain yield, grain quality, and morphophysiological parameters of three winter wheat cultivars: Kilimanjaro, Hymalaya, and Ostroga. The cultivars were grown in crop rotation after grain maize harvest, using three tillage systems: conventional (C), reduced (R), and no-tillage (N). A three-year field experiment was conducted in southeastern Poland. Compared to no-tillage, the use of conventional and reduced systems resulted in higher grain yield, increased leaf area index and relative chlorophyll content, and higher gas exchange parameters. In the conventional system, the highest grain yield was achieved by cvs. Hymalaya and Ostroga, while in no-tillage and reduced, it was cv. Hymalaya. Compared to no-tillage, the conventional system resulted in higher values of grain quality parameters, while simultaneously reducing ash content, and the reduced system promoted a better gluten index. Interactions between cultivar and tillage system demonstrated good grain quality in terms of protein, falling number, and gluten index. Gluten content above 25.0% was found in grains of cvs. Kilimanjaro and Hymalaya in the reduced and conventional systems, and cv. Ostroga in the conventional system. The dry and semi-drought periods in the 2018/2019 season were conducive to more favorable grain quality parameter values: protein, gluten, falling number, and ash. However, the resulting grain was characterized by a lower gluten index and lower physical parameters. Cvs. Hymalaya and Ostroga are recommended for cultivation in conventional and reduced tillage systems, and cv. additionally for no-tillage systems. Growing the cv. Kilimanjaro in no-tillage and reduced tillage systems, and the cv. Ostroga in a no-tillage system, will result in lower grain yields. Full article