Search Results (46)

Milk production in dairy herds is determined by both intrinsic and extrinsic factors, with reproductive efficiency serving as a primary determinant. Infectious, nutritional, and management-related challenges can reduce this efficiency. Following parturition, cows are more susceptible to clinical disorders due to a temporary loss of integrity in the cervix, vagina, and vulva, which allows environmental bacteria to ascend and alter the vaginal microbiota. These microbial changes may disrupt endocrine responses related to conception and contribute to repeat breeder cow syndrome (RBCS), which is defined as failure to conceive after three or more inseminations. This study investigated associations among cultivable vaginal bacteria, circulating progesterone and glucose concentrations, and reproductive performance in 30 fourth-parity Holstein cows with a body condition score of 3.5. Cows were classified by reproductive history as repeat breeders (RBCS; n = 14) or controls (CTL; n = 16). Vaginal mucosal samples were collected at insemination and cultured on blood agar and MacConkey media under aerobic and microaerobic conditions. Bacterial identification was conducted using Gram staining and standard biochemical assays. Blood samples were collected at insemination, on day 5 post-insemination, and every two days thereafter to measure progesterone and glucose concentrations. Fertility outcomes were analyzed using PROC GLIMMIX, and hormonal data were analyzed using mixed models with repeated measures. The bacterial genera identified included Bacillus, Escherichia coli, Staphylococcus, Klebsiella, Proteus, Streptococcus, and Actinomyces. Progesterone and glucose concentrations did not differ significantly between groups (p > 0.05). However, the fertility rate (p < 0.05; CTL:87.50% vs. RBCS:57.14%) and number of attempts to conceive (p < 0.001; CTL:2.5 vs. RBCS:6.7) differed statistically between treatments. A higher prevalence of S. hyicus was detected in RBCS cows, and E. coli, S. hyicus, and Proteus spp. were more frequently detected in non-pregnant cows. These findings suggest that the identified cultivable vaginal bacteria are associated with reproductive status in dairy cows. Full article

The growing demand for resources for production in intensive livestock farming requires research to operate with an environmentally sustainable perspective and respect for animal welfare, promoting circularity in the livestock industry. In this context, animal monitoring plays a key role in livestock management, not only to ensure their well-being but also to preserve the balance of the territory. In particular, early detection of oestrus events is one of the crucial elements in livestock monitoring. This study presents the development and on-farm validation of a low-power oestrus detection system for dairy cows, based on stand-alone smart pedometers (SASPs) connected through a Low-Power Wide-Area Network (LPWAN). The system implements an upgradeable, threshold-based algorithm that analyzes cow motor activity using a 24 h moving-mean approach and three behavioral indicators related to oestrus expression. Data are processed on board and transmitted to a cloud platform for visualization through a farmer-oriented WebApp, without requiring any fixed installation in the barn. The system was tested on a commercial free-stall dairy farm over three experimental campaigns (2021–2023). Oestrus events were validated through farmer visual observation and milk progesterone analysis, used as the reference method. A total of 22 confirmed oestrus events were analyzed. The system achieved a detection rate of 72.7% for certain oestrus events and 86.4% when including probable detections, with a mean oestrus duration of 18.1 ± 2.5 h, consistent with values reported in the literature. The proposed solution demonstrates the feasibility of a transparent, low-computational-cost oestrus detection approach compatible with LPWAN constraints. Its plug-and-play design, reduced infrastructure requirements, and upgradable firmware, although not able to self-update, limiting its potential compared to the machine learning-based methods present in the literature, make it suitable for practical adoption, particularly in farms where conventional connectivity and high-cost commercial systems are limiting factors. Full article

The aim of this study was to evaluate the effect of the linoleic–α-linolenic acid ratio (LA:ALA) on cyclicity, oocyte quality, early pregnancy parameters, milk yield, and composition. Holstein cows were randomized to a 6:1-LA:ALA diet (Low-OMG3: n = 3 pens; 11 primiparous, 14 multiparous) or a 2:1-LA:ALA diet (High-OMG3: n = 3 pens; 10 primiparous, 14 multiparous). Diets were isocaloric and isonitrogenous and fed between 15 and 140 days in milk (DIM). Data were compared using linear mixed models. As expected, omega-3 concentrations in milk and blood increased in the High- compared to Low-OMG3 cows. No effect of diet was observed on cyclicity by 45DIM or oocyte quality at 50DIM. High-OMG3 cows had larger corpus luteum size (11–32 d post-timed artificial insemination [TAI]) and greater blood flow (32–60 d post-TAI) than Low-OMG3 cows. However, there was no effect of diet on progesterone, pregnancy-associated glycoproteins, or conceptus size. High-OMG3 cows produced more milk throughout the study, had greater lactose, and tended to have greater protein yield at 50DIM. In conclusion, decreasing the LA:ALA dietary ratio in lactating dairy cows did not provide evidence of effects on cyclicity, oocyte quality, or other early pregnancy-related parameters, but affected corpus luteum size and blood flow, enhanced milk production, and partially increased protein and lactose yields. Full article

The present study aimed to evaluate the effects of fermented compound Chinese herbal medicine (FCHM, Vaccaria segetali, Tetrapanax papyriferus, Ligusticum chuanxiong Hort and Rhaponticum uniflorum) on the reproductive performance of Ningxiang sows. A total of 30 Ningxiang sows were randomly assigned to the control group (CON), CHM group, and FCHM group. The results indicated that dietary CHMs and FCHM supplementation significantly increased (p < 0.05) the milk production, feed intake, reproductive performance protein content, and lactose content in the milk of sows and significantly decreased (p < 0.05) the number of somatic cells in colostrum and in the number of low-birthweight piglets. Dietary FCHM supplementation significantly increased (p < 0.05) the number of healthy piglets, birth litter weight, colostrum fat content, and feed intake. Moreover, malondialdehyde, porcine interleukin-1α, and porcine interleukin-6 in sow serum were decreased (p < 0.05), and the activity of superoxide dismutase, total antioxidant capacity, immunoglobulin, prolactin and progesterone levels (p < 0.05) were increased, with FCHM supplementation. The colostrum metabolomics analyses showed that FCHM significantly enriched the oxytocin signaling pathway, calcium signaling pathway, and pathways associated with milk composition synthesis. In conclusion, supplementing with FCHM improved the reproductive performance and milk metabolic biomarkers of sows and may serve as an effective feed additive to improve productivity. Full article

This study examined the effects of long-term Saccharomyces cerevisiae supplementation on feed intake, milk production, milk composition, and selected reproductive indicators in lactating dairy cows. Twenty-four multiparous Holstein–Friesian crossbred cows were blocked by parity and randomly allocated to three treatments: a control group without supplementation (CON; n = 7), live yeast supplementation for 60 days (YS-60; n = 10), and live yeast supplementation for 90 days (YS-90; n = 7). Dry matter intake and body weight gain were significantly higher in cows receiving live yeast, with the greatest responses observed in the YS-90 group (p < 0.05). Milk yield and energy-corrected milk were increased by supplementation, particularly in YS-90 cows (p < 0.01), along with higher milk fat and lactose concentrations. Somatic cell count was consistently lower in YS-90 cows throughout the 14-week experimental period. Body condition score differed among treatments (p < 0.01), with higher values observed in yeast-supplemented cows. Feed efficiency did not differ among treatments. Reproductive parameters, including estrus detection and pregnancy rate, were not significantly affected by live yeast supplementation, although plasma progesterone concentration was higher in supplemented cows (p < 0.05). Given the limited number of animals per treatment, reproductive outcomes should be interpreted cautiously. Overall, extended live yeast supplementation improved production performance and udder health, while its effects on reproductive performance warrant further investigation. Full article

Background: Against the backdrop of the large-scale and intensive development of the livestock industry, enhancing the reproductive efficiency of cattle has become a crucial factor in industrial development. Holstein cows, as the most predominant dairy cattle breed globally, are characterized by high milk yield and excellent milk quality. However, their reproductive efficiency is comprehensively influenced by a variety of complex factors, and improving their reproductive performance faces numerous challenges. The ovary, as the core organ of the female reproductive system, plays a decisive role in embryonic development and pregnancy maintenance. It is not only the site where eggs are produced and developed but it also regulates the cow’s estrous cycle, ovulation process, and the establishment and maintenance of pregnancy by secreting various hormones. The normal functioning of the ovary is crucial for the smooth development of the embryo and the successful maintenance of pregnancy. Methods: Currently, traditional sequencing technologies have obvious limitations in deciphering ovarian function and reproductive regulatory mechanisms. To overcome the bottlenecks of traditional sequencing technologies, this study selected Holstein cows as the research subjects. Ovarian samples were collected from one pregnant and one non-pregnant Holstein cow, and single-nucleus transcriptome sequencing technology was used to conduct an in-depth study on the ovarian cells of Holstein cows. Results: By constructing a cell type-specific molecular atlas of the ovaries, nine different cell types were successfully identified. This study compared the proportions of ovarian cell types under different physiological states and found that the proportion of endothelial cells decreased during pregnancy, while the proportions of granulosa cells and luteal cells increased significantly. In terms of functional enrichment analysis, oocytes during both pregnancy and non-pregnancy play roles in the “cell cycle” and “homologous recombination” pathways. However, non-pregnant oocytes are also involved in the “progesterone-mediated oocyte maturation” pathway. Luteal cells during pregnancy mainly function in the “cortisol synthesis and secretion” and “ovarian steroidogenesis” pathways; non-pregnant luteal cells are mainly enriched in pathway processes such as the “AMPK signaling pathway”, “pyrimidine metabolism”, and “nucleotide metabolism”. Cell communication analysis reveals that there are 51 signaling pathways involved in the pregnant ovary, with endothelial cells, granulosa cells, and luteal cells serving as the core communication hubs. In the non-pregnant ovary, there are 48 pathways, and the interaction between endothelial cells and stromal cells is the dominant mode. Conclusions: This study provides new insights into the regulatory mechanisms of reproductive efficiency in Holstein cows. The differences in the proportions of ovarian cell types, functional pathways, and cell communication patterns under different physiological states, especially the increase in the proportions of granulosa cells and luteal cells during pregnancy and the specificity of related functional pathways, indicate that these cells play a crucial role in the reproductive process of cows. These findings also highlight the importance of ovarian cells in pathways such as “cell cycle”, “homologous recombination”, and “progesterone-mediated oocyte maturation”, as well as the cell communication mechanisms in regulating ovarian function and reproductive performance. Full article

Obesity is a multifactorial condition that influences metabolic, endocrine, inflammatory, circadian, and behavioral systems. These disruptions can adversely affect the initiation of lactogenesis II—the critical process marking the onset of copious milk secretion following childbirth. In mothers with obesity, prolonged inflammation within the mammary gland, a blunted hormonal response (notably of prolactin), altered progesterone and estrogen dynamics, high leptin levels, and misaligned circadian rhythms contribute significantly to delayed lactogenesis. In addition, mechanical difficulties and psychological factors further hinder effective breastfeeding. This report synthesizes evidence from human epidemiological studies and animal models that elucidate the diverse mechanisms linking maternal obesity to delayed lactogenesis. We review the role of obesity-associated inflammatory mediators in impairing mammary tissue remodeling, the endocrine aberrations that impair lactogenic signaling, the consequences of circadian disruption on hormonal rhythmicity, and the behavioral influences that challenge effective breastfeeding. Finally, we discuss the clinical implications of these findings and propose future research directions targeting endocrine modulation, anti-inflammatory therapy, circadian interventions, and enhanced lactation support strategies for mothers with obesity. Full article

This study evaluated the effects of supplementing early-lactation Holstein cows with rumen-protected omega-3 fatty acids (calcium salts) on productive and reproductive performance. Thirty-six multiparous cows were randomly assigned to one of two treatments from 21 ± 2 days before calving to 105 ± 5 days in milk (DIM): a Control group (C) or an Omega-3-supplemented group (O-3), receiving a blend of linseed and fish oil (60:40). Both groups were fed isoenergetic diets, with ground corn as the control supplement. Total dry matter and net energy intake did not differ between treatments. A treatment-by-time interaction was observed for milk yield, with cows in the O-3 group producing more milk than controls at specific time points. Additionally, O-3 cows had higher overall protein yield and improved feed efficiency. No differences were found in body weight, BCS, glucose, insulin, IGF-1, or urea concentrations, but a tendency toward higher plasma NEFA and BHBA concentrations and lower energy balance was observed in the O-3 group. Supplementation increased plasma cholesterol and progesterone concentrations and was associated with a higher proportion of cows being pregnant at 130 DIM. These findings suggest that omega-3 supplementation may improve specific aspects of lactational performance and reproductive efficiency without compromising metabolic status. Full article

In dairy cattle, ovarian cysts (COFs) represent a major cause of infertility. They can be divided morphologically into follicular and luteal cysts based on their wall thickness, which can be examined by ultrasound, and progesterone secretion, which can be analyzed in serum or milk. The aim of our study was to evaluate cyst recovery using a modified ovsynch protocol with no need to differentiate COFs. Additionally, the beta-hydroxybutyric acid level (BHB), progesterone values, and trace elements in the serum were measured when therapy started. Fourteen days after treatment, COF recovery was confirmed in 88% of the cases. The median calving-to-conception interval, number of artificial inseminations until pregnancy, and median number of days from treatment to pregnancy were not different between the modified ovsynch protocol group and all other COF treatments. The logistic regression for COF included the parameters group, the cyst type, breed, the number of artificial inseminations (AIs), calving to conception cut at 200 d p.p., the cyst size, and therapy. The backward (and also forward) variable selection of the logistic regression yielded only the cyst size as a significant negative impact factor for recovery. In conclusion, the modified ovsynch protocol is a useful, practical option for COF treatment with the advantage of not needing to differentiate between the two cyst types. Full article

This study investigated changes in physiological processes and rumen microbial communities in does from mid-gestation to lactation and identified potential associations between these physiological changes and the rumen microbiome. Additionally, we studied the transmission mechanisms of microorganisms between the dam and offspring. Our study demonstrates significant changes in maternal physiological metabolism, immune status, and rumen microbiota from mid-pregnancy through lactation. We identified potential associations between these physiological changes and the rumen microbiome. Moreover, the findings highlight that alterations in maternal physiological metabolism and immune status significantly influence the growth and immune development of offspring kids. Additionally, we observed that the maternal microbiota serves as a key source of gastrointestinal microbial communities in young animals, with early colonization of maternally derived microbes in the offspring’s gastrointestinal tract playing a role in shaping their immune system development. The results for primary outcomes are as follows: The serum levels of estrogen and progesterone in pregnant does were greater than those observed during lactation, while the concentration of growth hormone, triiodothyronine, and glucose exhibited an upward trend during lactation. During late gestation, the serum IL-10 concentration in does decreased, while the TNF-α concentration increased. Additionally, on day 140 of gestation, does showed a significant decrease in IgG, total protein, and globulin levels. From mid-gestation to lactation, the abundance of dominant phyla and genera, including Firmicutes, Bacteroidetes, Patescibacteria, Bacteroidales_RF16_group, Clostridia_UCG-014, RF39, and Eubacterium_ventriosum_group, in the rumen of does underwent significant changes. LEfSe analysis identified a series of marker microorganisms in the rumen of does at different physiological stages. A correlation was observed between these dominant bacteria and the serum physiological indicators of the does. Notably, rumen volatile fatty acids also exhibited a correlation with serum physiological indicators. In addition, serum physiological indicators of does were significantly correlated with the growth and immune indicators of their kids. Microbiological origin analysis revealed that the gastrointestinal microbiome of kids primarily originated from the rumen, birth canal, and milk of does. Further analysis identified a correlation between the kids’ serum immunometric indicators and certain gastrointestinal microorganisms. In particular, the jejunum microbiota of 28-day-old lactating kids, including Alysiella, Neisseria, and Muribaculaceae, showed a significant positive correlation with serum IL-6 and IL-10 levels. Meanwhile, these genera were dominant in the saliva and milk of does, suggesting a direct microbial transfer from dam to offspring. These microbial communities may play a significant role in modulating the metabolism and immune responses of the offspring, thereby influencing their immune system development. Full article

Olive cake was incorporated at a low inclusion rate (3.7%) into the rations of dairy cows through partial substitution of maize, and its effects on milk production, general health, and fertility traits were investigated. Multiparous purebred Holstein dairy cows (n = 148) were divided into two groups: a treated group (n = 86) and a control group (n = 62). The control ration (group C) was modified by replacing 1 kg of maize with an equal quantity of olive cake (group T). The experiment lasted from 60 days prepartum to 150 days postpartum. Electronic aids were utilized to quantify daily milk yield and detect estrus expression, while clinical and uterine examinations were performed weekly. Blood samples, uterine epithelial cells, and endometrial tissue samples were collected from a subgroup of healthy animals in both groups at specific time points. Blood samples were examined to determine the β-hydroxybutyric acid (BHBA), acute phase proteins (haptoglobin [Hpt] and serum amyloid A [SAA]), progesterone, and pregnancy-associated glycoproteins concentrations. Uterine epithelial cells were assessed for polymorphonuclear neutrophil (PMN) counts, and the expression of nine genes encoding inflammatory cytokines and immune system activation was analyzed in uterine biopsy tissue. No significant differences (p > 0.05) were observed between groups in milk yield, general morbidity, clinical endometritis, or conception rates. However, animals in group T came to estrus approximately 6 days earlier (p = 0.013) than those in group C; progesterone concentrations on day 7 of the subsequent cycle tended (p = 0.07) to be higher in group T. On day 21, BHBA concentrations were higher in group C than in group T (p < 0.05). Throughout the experiment, Hpt levels in group T were consistently lower (p < 0.001), while SAA was lower on day 7 compared to group C. From days 21 to 42 postpartum, there was a significant reduction in PMN numbers (group C p = 0.02; group T p < 0.0001), with a tendency for a greater reduction rate in group T (p = 0.08). Among the genes studied, a significant difference was revealed in the expression of the ILA1 gene, with strong correlations of gene expression in group C and weak to moderate correlations in group T. In conclusion, under the conditions of this experiment, the inclusion of olive cake into dairy cows’ rations did not affect milk production but improved certain health and fertility parameters, making olive cake a suitable alternative feedstuff for high-producing animals. Full article

Adequate milk production is key for optimal infant growth, yet women often wean due to perceived low milk production (LMP). Maternal adiposity and gestational diabetes mellitus (GDM) are known potential risk factors for LMP and may alter both hormonal and macronutrient composition of human milk. This study aims to investigate the hormonal and macronutrient composition of human milk in relation to LMP, particularly in the context of maternal adiposity and GDM. Human milk samples were collected from 68 women with LMP and 160 women with normal milk production during 1–6 months postpartum. Of the 228 participants with the mean pre-pregnancy BMI of 26.3 ± 6.0 kg/m², 80 (35.1%) had GDM. Concentrations of hormones (estrone, estradiol, progesterone, insulin, leptin, adiponectin) and macronutrients (fat, protein, lactose, glucose) were measured and compared between the two groups. Univariable and multivariable logistic regression analyses were conducted, adjusting for potential confounders such as maternal body mass index (BMI) and GDM, to assess the relationships between hormone and macronutrient concentrations, and milk production. Preliminary analyses indicated that higher concentrations of estrone (p < 0.001), leptin (p = 0.009), insulin (p = 0.002), protein (p = 0.012), and lactose (p = 0.004) were associated with LMP. After adjusting for maternal BMI and GDM in the univariable models, all of these associations remained, and progesterone (p = 0.045) also emerged as positively associated with LMP. In multivariable regression, followed by stepwise model selection, estrone (p < 0.001), protein (p < 0.001) and lactose (p = 0.015) demonstrated the strongest associations with LMP, with maternal BMI also contributing significantly (p = 0.010). The findings suggest that hormone and macronutrient concentrations in human milk may reflect LMP status and provide insights into the underlying biological mechanisms associated with LMP. Full article

Lactation in dairy cows implies comprehensive endocrine and metabolic changes including a systemic electrolytic reaction. Previous studies have rarely considered these specific demands due to the influence of lactation periods. Therefore, this study aimed to assess the effects of early, middle, and late lactation phases on the dynamic changes in serum concentrations of progesterone (P4), 17β-oestradiol (E2), cortisol, and some electrolytes (Ca⁺⁺, Mg⁺⁺, Na⁺, K⁺, Cl⁻, Pi) and biochemical parameters (alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), total bilirubin, urea, and iron (Fe⁺⁺) in 10 Holstein, 10 Brown Swiss, and 10 Modicana multiparous healthy dairy cows (4.2 ± 1.7 years of age) sampled at 60-day intervals throughout lactation. Lactation induced significant changes in the concentrations of P4, which peaked at >120–180 days, decreased at >240–300 days, and increased again after 300 days. Cortisol showed an opposite trend to P4, with concentrations progressively decreasing, except for the phase between >240 and 300 days, and a steep drop at >300 days compared to previous phases. Na⁺ concentrations showed the lowest values at 0–60 d and the highest ones at >180–240 days, whereas Mg⁺⁺ showed the highest values at >60–120 d and the lowest at >300 d. Significant correlations were found between P4 with cortisol, Cl⁻ and K⁺, and cortisol with Ca⁺⁺ and LDH. Significant differences in average concentrations of AST, ALT, LDH, Ca⁺⁺, Mg⁺⁺, and Fe⁺⁺ were observed among different dairy cow breeds. Understanding the dynamic changes in hormone levels, electrolytes, and biochemical parameters during different lactation phases, while considering breed differences in dairy cows, is crucial for improving herd health management and milk production in commercial dairy farms. Full article

Endometrial disorders, such as infertility and endometriosis, significantly impact reproductive health, thus necessitating better models to study endometrial function. Current in vitro models fail to replicate the complexity of the human endometrium throughout the entire menstrual cycle. This study aimed to assess the physiological response of human endometrial organoids (hEOs) to in vitro hormonal treatments designed to mimic the hormonal fluctuations of the menstrual cycle. Endometrial biopsies from three healthy women were used to develop hEOs, which were treated over 28 days with three hormonal stimulation strategies: (1) estrogen only (E) to mimic the proliferative phase, (2) the addition of progesterone (EP) to simulate the secretory phase, and (3) the further addition of cAMP (EPC) to enhance the secretory functions of hEOs. Gene and protein expression were analyzed using qPCR, IHC, and ELISA. The hEOs exhibited proliferation, gland formation, and appropriate expression of markers such as E-cadherin and Ki67. The hormonal treatments induced significant changes in PR, HSD17B1, PAEP, SPP1, and other genes relevant to endometrial function, closely mirroring in vivo physiological responses. The prominent changes were observed in EPC-treated hEOs (week 4) with significantly high expression of uterine milk components such as glycodelin (PAEP) and osteopontin (SPP1), reflecting mid- to late-secretory phase physiology. This model successfully recapitulates human menstrual cycle dynamics and offers a promising platform for studying endometrial disorders and advancing personalized treatments in gynecology. Full article

Activities such as childbirth and breastfeeding can cause severe oxidative stress and inflammatory damage to the mother during early lactation, and can affect animal milk production, and the growth and development of offspring. Trehalose alleviates damage to the body by endowing it with stress resistance. In this study, we used trehalose combined with Lactobacillus plantarum, Bifidobacterium longum, Bacillus subtilis, and Saccharomyces cerevisiae to explore whether dietary intervention can alleviate oxidative stress and inflammatory damage in early lactation and to evaluate the growth ability, acid production ability, antioxidant ability, non-specific adhesion ability, antibacterial ability, and other parameters to determine the optimal combinations and proportions. The results showed that the synbiotics composed of 2.5% trehalose and 1 × 10⁷ cfu/g of Bifidobacterium longum could regulate the gut microbiota, and promote mammary gland development in dams by reducing progesterone (PROG) content in the blood, increasing prolactin (PRL) and insulin-like growth factor-1 (IGF-1) content, enhancing their antioxidant and immune abilities, and effectively increasing the weight and lactation of early lactating dams. In addition, it can also affect the growth of offspring and the development of the intestinal barrier. These results indicate that trehalose synbiotics have great potential in alleviating oxidative stress and inflammatory damage in early lactation. Full article