Search Results (461)

Fluoxetine and carbamazepine are widely prescribed psychotropic drugs, yet few studies have quantified metal(loid) impurities in these medicines, which may pose health risks to patients. This study aimed to determine concentrations of As, Cd, Cr, Cu, Fe, K, Mg, Mn, P, Pb, Se, and Zn in brand, similar, and generic samples of fluoxetine and carbamazepine marketed in Campo Grande, Brazil. Drug samples were purchased from local pharmacies, digested with acid, and analyzed by Inductively Coupled Plasma Optical Emission Spectrometry (ICP OES). Results showed that arsenic was detected only in fluoxetine samples, with concentrations ranging from 0.068 to 0.217 mg/kg, all below national and international limits. Phosphorus presented the highest levels, especially in fluoxetine, reaching up to 14,000 mg/kg, and up to 93 mg/kg in carbamazepine. Other elements such as Fe (0.07–3.03 mg/kg), Mg (0.21–259 mg/kg), K (up to 45 mg/kg), Se (up to 1.5 mg/kg), and Zn (up to 4.2 mg/kg) were also quantified, while Cd, Cr, Cu, and Pb were below detection limits. The hazard index (HI) exceeded 1 for all carbamazepine samples and for one brand, two similar, and three generic fluoxetine samples, indicating that the intake of these medications may pose potential health concerns. These findings underscore the need for stricter monitoring of metal(loid) impurities in psychotropic drugs to protect patient safety and ensure regulatory compliance. Full article

This study delves into the necessity for gender-affirming practices, particularly focusing on the underrepresented transgender and non-binary communities in California’s Central Valley. Despite the recognized standards by the World Professional Association for Transgender Health (WPATH) for best practices in mental health care, adequately trained professionals in this region remains a notable scarcity. The paper highlights the heightened risks these communities face, including discrimination and mental health challenges, underscoring the critical need for compassionate and competent care. The research aims to bridge the gap in education and training for practitioners on gender diversity and improve mental health services for transgender and non-binary individuals. Through thematic analysis of individual interviews, the study captures the experiences of gender diverse individuals with behavioral health care, emphasizing the importance of gender-affirming care, the dangers of pathologizing gender diversity, and the adverse impacts of gatekeeping and conversion therapy. Conclusively, the study advocates for an informed consent model for medical transitions, as per WPATH guidelines, and calls for a shift towards intersectional, inclusive practices. It stresses the need for ongoing education, policy reform, and advocacy to ensure equitable, affirming mental health care for gender diverse populations. Full article

Current research on Daoist neidan (內丹, Internal Alchemy) has primarily focused on its philosophical frameworks, practical methods, and therapeutic benefits; however, systematic inquiry into the mechanisms of failure during practice remains limited. This study investigates the long-neglected yet pivotal phenomenon of zouhuo rumo (走火入魔, fire deviation and entry into demonic states) within Daoist cultivation, especially as it emerges in the context of dual cultivation of xing and ming (性命雙修). Through textual and hermeneutical analysis, this study traces the historical evolution, semantic transformation, and causal structure of the term, revealing its dual function as both a technical deviation and a religious warning. Findings indicate that zouhuo rumo arises from the interplay of impure self-refinement, loss of mental focus, improper fire phases (火候), and illusory disturbances, reflecting a profound psychosomatic imbalance rooted in the practitioner’s mind-nature (心性). Daoism interprets this state as mokao (魔考, demonic trials in Daoist cultivation), a transformative mechanism designed to refine inner alignment. On this basis, this study proposes a three-stage healing pathway—Spirit Preservation and Breath Stabilization (存神定息), Inner Vision and Self-Reflection (內觀返照), and Transformation of Form and Refinement of Essence (化形改質)—and constructs a Daoist cultural healing model that integrates moral cultivation, breath regulation, and introspection. This model provides a non-pathologizing cultural framework for enhancing psychological resilience, reconstructing meaning, and addressing contemporary spiritual and psychological crises. Full article

Neurodegenerative and neuropsychiatric disorders remain a major public health concern due to their progressive nature, high prevalence, and considerable socioeconomic burden. Conventional treatments often fall short, facing limitations such as pharmacoresistance, adverse effects, and limited efficacy, underscoring the need for complementary approaches. Recent advances highlight the central role of the gut–brain axis (GBA) in neurological health, positioning psychobiotics and probiotic strains with potential mental health benefits, as candidates in adjunctive therapy. This review integrates current evidence on the GBA’s involvement in conditions such as Alzheimer’s disease, Parkinson’s disease, depression, and anxiety. We examine how psychobiotics may modulate neuroinflammation, oxidative stress, and neurotransmitter signaling, thereby contributing to cognitive and emotional regulation. Both preclinical and clinical studies are discussed, with emphasis on biomarker changes, quality-of-life outcomes, and neuropsychiatric comorbidities. We also explore recent innovations, including precision psychobiotics, microbiota–drug synergies, and their relevance to overlapping metabolic and neurodegenerative pathologies. Finally, we address the major translational challenges in the field, strain selection, methodological standardization, biomarker integration, and ethical design, highlighting key perspectives for advancing psychobiotics research toward clinical application. Full article

Background: Post-traumatic stress disorder (PTSD) is a mental disorder that can develop after experiencing or witnessing a traumatic event. Dysfunction of the hypothalamic–pituitary–adrenal (HPA) axis and alterations in neurotransmitters (gamma-aminobutyric acid (GABA) and glutamate) are the main pathologies of PTSD. In particular, altered GABAergic neurotransmission and reduced GABA activity are linked to PTSD. Given the low efficacy and side effects of serotonin reuptake inhibitors—the most common treatment for PTSD—a safer and more effective treatment is urgently needed. Bojungikgi-tang (BJIGT) is well-known herbal prescription in East Asia, which used to boost immunity and to alleviated symptoms such as chronic fatigue, poor appetite, and indigestion. However, its role in PTSD remains largely unexamined. This study aimed to investigate the effects of BJIGT in single-prolonged stress with shock (SPSS)-induced PTSD male mice for 2 weeks. Methods: To assess PTSD-like behaviors, we conducted open field, forced swimming, Y-maze, and contextual fear conditioning tests. To investigate the underlying mechanisms, we performed ELISA, Western blot, and immunohistochemistry. Results: BJIGT significantly ameliorated PTSD-like behaviors, including emotional and cognitive decline. Additionally, it restored serum corticosterone levels, regulated neuronal functions (c-Fos, DCX, and Prox1), and GABAergic neurotransmission-related factors (vGAT, GAD67, and parvalbumin) in the hippocampus of PTSD mice. Notably, in SPSS-induced PTSD mice, BJIGT effectively ameliorated pathological changes by modulating JNK-CaMKII and Pin1–β-catenin intracellular signaling. Conclusions: These findings revealed that BJIGT effectively improved PTSD-like emotional and cognitive decline by regulating the HPA axis and GABAergic neurotransmission in SPSS-induced PTSD mice, thereby promising to be an effective strategy for the treatment of PTSD. Full article

Exposure to campus suicide poses a significant threat to adolescent mental health. While childhood psychological abuse (CPA) is a known vulnerability factor for depression, the mechanisms linking this early adversity to depressive symptoms (DS) following acute trauma remain unclear. This study aimed to test a chain mediation model where CPA contributes to DS through the sequential effects of psychological trauma (PT) and anxiety symptoms (AS). In a cross-sectional study of 1603 adolescents exposed to a campus suicide event, participants completed self-report measures for CPA, PT, AS, and DS. Chain mediation analysis revealed a significant direct effect of CPA on DS. More importantly, the hypothesized chain mediation pathway (CPA → PT → AS → DS) was significant and was identified as the most substantial indirect route. A key asymmetry emerged: the direct effect of CPA on DS remained robust, whereas its direct effect on AS became non-significant when controlling for DS. These findings suggest that CPA establishes a specific vulnerability to depression that, when activated by an acute stressor, initiates a pathological cascade. Interventions for suicide-exposed youth should be trauma-informed, prioritizing those with a CPA history and targeting emergent anxiety to interrupt the progression to severe depression. Full article

Background/Objectives: Myotonic dystrophy type 1 (DM1) is a multisystem disorder that affects the central nervous system. Despite previous studies, blood-based biomarkers have not been sufficiently characterized. This study investigated plasma neurofilament light chain (NfL), phosphorylated tau (p-tau181), amyloid-β (Aβ42/40), and glial fibrillary acidic protein (GFAP) in a Japanese cohort with DM1 to assess their potential as biomarkers. Methods: Forty patients with genetically confirmed DM1 were enrolled in this study. Plasma NfL, p-tau181, Aβ42/40, and GFAP were quantified using single-molecule array technology. Clinical and genetic variables, including age, CTG repeat size, Mini-Mental State Examination (MMSE) score, modified Rankin Scale (mRS) score, and creatine kinase levels, were analyzed for correlations. Results: NfL and p-tau181 were significantly elevated in patients with DM1 compared with controls, with 95% exceeding the p-tau181 cut-off. NfL was moderately correlated with age, age at onset, and mRS, and no significant associations were observed between p-tau181 and other biomarkers, although a correlation was noted with serum creatine kinase. Conclusions: These findings support that NfL is a marker of disease severity in DM1 and identified plasma p-tau181 as a potential novel biomarker. While the mechanisms underlying the increased p-tau181 levels remain unclear, they may reflect DM1-related pathologies in the brain and possibly in skeletal muscle. Study limitations include a small sample size and lack of age-matched controls, highlighting the need for longitudinal validation. This study demonstrates the utility of NfL and suggests that p-tau181 is an emerging biomarker for DM1, supporting future work toward biomarker-guided monitoring and therapeutic evaluation. Full article

The endocannabinoid system (ECS) is a primary regulatory system in human physiology that serves to help maintain homeostasis throughout the nervous system, immune system, and gastrointestinal system. This review has the goal of evaluating the unique opportunity for the ECS to provide a regulatory axis within the microbiota–gut–brain axis, particularly with regard to neurodevelopment, immune tolerance, and gut health. Cannabinoid receptors CB1 and CB2 and endogenous ligands anandamide (AEA) and 2-arachidonoylglycerol (2-AG have the ability to provide a variety of signaling pathways that can regulate cognitive resilience, emotional tuning, and immune regulation. Because the ECS has the ability to regulate multiple neurochemicals, alter immune cell functions, and maintain gut barriers, the ECS exists at the crossroads of many physiological systems, which also have a predictive role in neurodegenerative disease, chronic inflammation, and mental illness. Our goal is to present the latest and best recent advances in the ECS literature and establish evidence that there exists some modest potential for the therapeutic modulation of the ECS to improve pathological manifestations of cross-system dysregulation. In addition to cellular signaling pathways, the ECS affects other homeostatic processes, such as synaptic plasticity and the level of neuroprotection in the CNS, immune-related homeostasis, and coordinating the composition of gut microbiota. We argue that the ECS represents a suitable new therapeutic target that could modulate dysregulation across these systems more inclusively. This paper aims to emphasize the proposed potential of the ECS’s position in this axis and propose advanced cannabinoid-based interventions as a novel mechanism for developing personalized medicine and health systems through multi-system integration. Full article

Empirical studies support the difficulties European youths encounter when transitioning into adulthood, as well as several economic and social constraints that make the acquisition of a full adult role complex and challenging, with relevant implications for psychological adjustment. In this direction, international research showed the effectiveness of Artificial Intelligence (AI) in promoting mental health adjustment, although most studies are limited to the field of clinical psychology for diagnosing and preventing the onset of pathological problems rather than on non-clinical populations. Furthermore, only a limited number of studies have been conducted in European Countries in comparison to Asian and American countries. Accordingly, this scoping review aims to provide an overview of studies conducted in Europe on AI applications for psychological support to promote psychological adjustment in emerging adults who face the challenges of reaching adulthood, often associated with stress and pressures that increase the likelihood of developing psychological problems. Out of 167 initially selected articles for the period between 2015 and 2025, only six articles were included for the final synthesis, according to explicit inclusion and exclusion criteria, and among them, only three studies were conducted on emerging European adults using different AI tools to provide support to their psychological adjustment. Results from these studies support, first, that despite the significant increase in the AI applications for mental health, their use is still scarce in the European context and specifically to promote emerging adults’ adjustment; second, that despite the scarce applications of AI tools in this sense, results from the few studies are promising regarding the potential AI applications. Future research should better investigate the effects of AI tools to understand their benefits in promoting the mental health of European youths, considering the challenges that they face in going through adulthood. Full article

Major Depressive Disorder (MDD) is a high-risk mental illness that severely affects individuals across all age groups. However, existing research lacks comprehensive analysis and utilization of brain topological features, making it challenging to reduce redundant connectivity while preserving depression-related biomarkers. This study proposes a brain network analysis and recognition algorithm based on class-specific correlation feature selection. Leveraging electroencephalogram monitoring as a more objective MDD detection tool, this study employs tensor sparse representation to reduce the dimensionality of functional brain network time-series data, extracting the most representative functional connectivity matrices. To mitigate the impact of redundant connections, a feature selection algorithm combining topologically aware maximum class-specific dynamic correlation and minimum redundancy is integrated, identifying an optimal feature subset that best distinguishes MDD patients from healthy controls. The selected features are then ranked by relevance and fed into a hybrid CNN-BiLSTM classifier. Experimental results demonstrate classification accuracies of 95.96% and 94.90% on the MODMA and PRED + CT datasets, respectively, significantly outperforming conventional methods. This study not only improves the accuracy of MDD identification but also enhances the clinical interpretability of feature selection results, offering novel perspectives for pathological MDD research and clinical diagnosis. Full article

Background: Current research is paying more attention to neurological outcomes and quality of life after life-threatening events. Children with heart disease are particularly vulnerable, especially after resuscitation events. While newer data show that adults with heart failure and a left-ventricular assist device suffer from a higher incidence of depression, mental health in pediatric heart disease patients is poorly understood. This is the first study in Germany to examine the quality of life and psychological burden in cardiac arrest survivors with congenital or acquired heart disease. Methods: This monocentric study retrospectively analyzed survival outcomes of pediatric heart disease patients who underwent in-hospital resuscitation between 2008 and 2022. The PedsQL and Strength and difficulties questionnaires were prospectively administered to survivors to assess quality of life and emotional/behavioral problems, while academic achievements were additionally documented. Results: Of 127 patients experiencing cardiac arrest, 91 (71.7%) survived to discharge. Most had complex congenital heart diseases; mean cardiopulmonary resuscitation duration was 14 min. Five patients received extracorporeal cardiopulmonary resuscitation. Of the 22 patients who were receiving follow-up care at the pediatric cardiology outpatient clinic at the time of the study, 14 completed questionnaires were received. Overall quality of life was comparable to healthy controls, though those with prolonged or multiple resuscitations showed lower physical, emotional, social, and school functioning scores. The Strengths and Difficulties Questionnaire revealed no pathological scores but elevated average values for hyperactivity and emotional problems in parent reports, and emotional and peer difficulties in self-reports, indicating increased psychological burden. Conclusions: While survival rates are comparable to international data, gaps exist in structured follow-up and neuropsychological care, especially for high-risk subgroups like ECMO survivors. Routine neuropsychological screening and multidisciplinary outpatient programs are essential to improve long-term follow-up care. Full article

Background: While sexual trauma is inherently an embodied experience, research on psychological interventions that is cognisant of geographic and socio-political community contexts within which embodied, therapeutic interventions occur remains limited. Decolonial, African and feminist community psychologies have noted this epistemic–ethical gap. Objectives: This paper explores the co-development of trauma-informed care pathways for adult survivors of childhood sexual trauma (CST) in under-resourced communities in Cape Town, South Africa. The study aimed to integrate intergenerational community knowledge, embodied therapeutic practices and collaborative approaches into locally relevant models of care. Methods: Drawing on feminist mental health frameworks, this qualitative study engaged 13 adult female survivors who identify as “coloured”. Embodiment was central in guiding the deconstructive therapeutic praxis, informing both the co-development of care pathways and the conceptualization of integrative trauma-informed care (ITIC) beyond pathologizing, deficit-based narratives. The cultivation of trust and the situated lived realities of survivors were foregrounded to illustrate the relational dimensions of trauma recovery. Results: Establishing relational safety emerged as the foundation for therapeutic engagement, supported by non-directive therapeutic probing. Grounding practices, affective regulation and embodied awareness enabled participants to process trauma at their own pace. Somatic engagement allowed the integration of dissociative experiences while strengthening relational resilience. Recovery was a continuous process, with participants reporting increased peace, authenticity and capacity for social connection despite structural barriers to community support. Conclusions: The development of care pathways was embedded within the research process itself, offering an approach that is culturally sensitive and responsive to survivors’ lived experiences. ITIC accounted for temporal, intergenerational and embodied trauma and should be adaptable across age and community-specific needs. The ITIC approach offers a transferable framework for co-developing de-pathologizing, culturally responsive interventions that can be adapted across diverse global contexts to support sustainable trauma integration. Full article

Background/Objectives: Hypericum perforatum L. (H. perforatum), commonly known as St. John’s wort, has been widely used in clinical practice to treat mental disorders. Previous studies and clinical applications have primarily focused on its alcohol-soluble ingredients. Our research was designed to investigate the physicochemical properties, antidepressant-like effects, and anti-post-traumatic stress disorder (PTSD)-like effects of the alcohol-insoluble polysaccharide-rich ingredients from H. perforatum. Meanwhile, the underlying mechanisms were elucidated. Methods: The physicochemical properties of two polysaccharide-rich ingredients, designated as HPP1 and HPP2, were characterized using colorimetric assay, capillary electrophoresis, high-performance gel permeation chromatography, and fourier transform infrared spectroscopy. Behavioral despair tests were conducted to rapidly assess and compare their antidepressant-like effects in mice. Subsequently, behavioral despair mice and foot-shock mice were established to thoroughly explore the impact of HPP2 on depression-like and PTSD-like symptoms. The effects of HPP2 on cerebral pathological changes, neurotrophic factors, and gut microbiota in foot-shock mice were detected through hematoxylin & eosin staining, immunofluorescence staining, and 16S rDNA (V3 + V4 regions) gene sequencing. Results: HPP1 and HPP2 are predominantly composed of arabinose, glucose, galactose, mannose, and galacturonic acid. The molecular weight distribution of HPP1 ranges from 1133 to 67,278 Da, whereas that of HPP2 extends from 1493 to 38,407 Da. Acute pre-treatment with HPP1 or HPP2 (200 mg/kg, i.g.) could reduce mice’s immobility in behavioral despair tests, with HPP2 exhibiting superior efficacy. Additionally, both acute and sub-chronic pre-treatment with HPP2 (50, 200, and 800 mg/kg, i.g.) effectively alleviated depression-like symptoms in behavioral despair mice. Prolonged pre-treatment with HPP2 (200 mg/kg, i.g.) also mitigated the slow increase in body weight and behavioral abnormalities in foot-shock mice. Furthermore, HPP2 (200 mg/kg) successfully restored hippocampal histomorphological abnormalities, neurotrophic disturbance, and dysregulation of the gut microbiota in foot-shock mice. Conclusions: HPP2 exerts noteworthy antidepressant-like and anti-PTSD-like impact in mouse models via multiple targets, indicating a potential therapeutic candidate in depression and PTSD therapy. Full article

Background: Since quality of life encompasses social, psychological, and physical well-being, it is a crucial component of overall health and well-being. The quality of life has a significant impact on both the mother and the unborn child throughout the perinatal period. Both parties suffer when a threat, such as an illness, materialises because it lowers the quality of life. Using the SCL-90-R and SF-36, the current study aims to investigate variations in anxiety levels and health-related quality of life (HRQoL) between pregnant women with and without relevant medical conditions. Methods: We carried out a cross-sectional study between April 2023 and December 2024. Eligibility criteria were: (a) pregnant women; (b) at least 18 years old; (c) of Romanian nationality residing in Romania; and (d) who signed informed consent and agreed to participate. A Personal Information Form (PIF), the SF-36 Health Survey, and the SCL-90-R questionnaire were used to collect data. Statistical analyses were performed with SPSS v26, using non-parametric tests (Mann–Whitney U, Spearman correlations). Results: Ninety-five of the 212 patients in the study reported having related medical conditions. There were no statistically significant differences between the groups in the physical or mental components of the SF-36. Nonetheless, the pathological group’s anxiety scores were noticeably higher. Particularly in the pathological group, Spearman correlation revealed an inverse relationship between anxiety and SF-36 physical component scores. Conclusions: The findings highlight the importance of integrating psychological screening into prenatal care, particularly for women with medical comorbidities. Early identification and management of elevated anxiety may help preserve maternal HRQoL and contribute to better perinatal outcomes. Full article

Liquid–liquid phase separation (LLPS) in cell biology has revolutionized our understanding of how cells organize biochemical reactions and structures through dynamic, membraneless organelles (MLOs). In neurons, LLPS-driven processes are particularly important for regulating synaptic plasticity, RNA metabolism, and responses to environmental stressors. Over the past decade, LLPS has gained increasing attention in neurobiology as a framework to interpret altered synaptic functions in various neurodevelopmental disorders (NDDs). These diseases comprise a diverse spectrum of clinical and pathological symptoms (e.g., global developmental delay, impaired cognitive and mental functions, as well as social withdrawal). Recent studies have highlighted how mutations in proteins containing intrinsically disordered regions (IDRs)—key drivers of LLPS—can alter condensate properties, resulting in persistent or defective MLO formation. These aberrant assemblies may disrupt RNA transport, splicing, and translation in developing neurons, thereby contributing to disorder pathology. IDRs are known to be enriched in membraneless components, such as stress granules, nuclear paraspeckles, and P-bodies, where they play crucial role in the formation, maintenance, and function of protein–RNA networks. This review explores the role of stress-induced MLOs in the nervous system, the molecular principles governing their formation, and how their dysfunction bridges the gap between environmental stress responses and neurodevelopmental impairment. Full article