Search Results (203)

Passive and fixed-damping transfemoral prostheses cannot adapt their resistance to the phase-dependent demands of human gait, and microprocessor-controlled commercial knees remain out of reach for most amputees. We present a fuzzy logic controller that modulates a magnetorheological (MR) damper directly from gait phase and knee joint angle, since linear state-feedback and discrete PI designs are valid only at a single linearization point and require retuning across the gait cycle. The controller is formalized as a fuzzy-basis-function expansion with established coverage and Lipschitz continuity; the universal-approximation property of Mamdani systems grounds fuzzy logic theoretically but does not certify this 8-rule controller’s performance, established empirically instead. A dissipativity-based Lyapunov argument and a numerical sweep establish local closed-loop stability and bounded, rate-limited actuation. The damper couples to the knee through a shaft–bearing–housing assembly sized by free-body and Goodman fatigue analysis and verified by finite-element analysis, with the control pipeline embedded on an ESP32 microcontroller in a 2 kg prototype, corresponding to Technology Readiness Level (TRL) 5–6. In a single-subject case study with one transfemoral amputee, the controller achieved the lowest mean RMSE (0.0557 over three trials) against a non-disabled gait reference among five compared conditions, improving on the best fixed voltage by 20.2% and a passive prosthesis by 6.8×; these are single-subject feasibility results, not a claim of generalizable performance. Full article

Hereditary hemolytic anemias (HHAs) have very similar clinical manifestations, complicating differential diagnosis and determining treatment strategies, such as splenectomy. To differentiate between hereditary spherocytosis (HS), pyruvate kinase deficiency (PKD), and hereditary stomatocytosis (HSt), we proposed measuring erythrocyte filterability using membrane filters with a pore diameter of 3 or 3.5 μm. Our modified method provides improved reproducibility, reducing the scattering in results caused by differences in the filters used. Erythrocytes’ filterability differs significantly between patients with HS, HSt, and PKD (medians and 95% confidence intervals were 0.025 [0.004; 0.100] (n = 86); 0.645 [0.520; 0.730] (n = 10), and 0.760 [0.700; 0.782] (n = 26), respectively). The specificity of the filterability test for the HS diagnosis was 99.1%, comparable with the best available tests, while its sensitivity (85.4%) was slightly lower than that of some alternative methods. Only the filterability test was able to identify a second subgroup of HS patients (14.0%) with higher filterability (close to normal), but the diagnosis of HS in these patients could be confirmed by reduced filterability on a 3-μm filter. This subgroup requires further detailed study. The proposed method for measuring filterability is promising for diagnosing HS. It has high sensitivity and specificity and can be performed quickly without expensive equipment. Full article

The study evaluated the effects of corn grain deterioration under different storage conditions in processed feeds and broiler digestibility. The corn grains were stored in chambers (quality corn and contaminated corn) at different storage temperatures and relative humidity (10 °C and 90%, 30 °C and 40%), and the evaluations were carried out at five storage times (zero, three, six, nine, and twelve months). After feed formulation (zero, six, and twelve months of corn stored), the broilers (504 animals) were fed for up to 14 days. The excreta collection was done twice per day for metabolism analysis. The 10 °C and 90% RH conditions influenced the loss of dry matter; the physical quality of corn grain was lower for the 30 °C and 90% RH conditions. When we obtained lower dry matter losses, the microbiological and mycotoxin levels were directly influenced by the loss of dry matter in corn. The storage time increased the risk of fungi (8.5 × 10⁷ CFU g⁻¹), bacteria (7.5 × 10⁶ CFU g⁻¹), and aflatoxins (64.96 µg kg⁻¹), reaching levels above acceptable limits for the corn quality and interfering with digestibility and animal metabolism. In addition, the storage time was the determining factor in the nutritional loss of corn quality, feed, and animal digestibility. Furthermore, the storage time reduced the levels of crude protein (5%), gross energy in the feed (3200 kcal kg⁻¹), metabolizable energy (3000 kcal kg⁻¹), and animal digestibility (3100 kcal kg⁻¹) after six months of storage. Based on the results of this study, the feed processing industry should pay close attention to the origin of stored corn and the associated environmental conditions, as these factors led to significant changes in grain quality, directly impacting animal nutrition and production. Therefore, it is recommended that the production chain invest more in the sector—specifically in adequate infrastructure and grain storage monitoring and control systems—to provide higher-quality raw materials to the processing industry. Full article

Exceptionally, this space is now dedicated to a tribute to Prof. Dr. Danilo Florentino Pereira (in memoriam), who passed away on 30 May 2026, during the organization period for the Special Issue “Application of Antimicrobial Peptides and Natural Products as Feed Additives in Animal Production” of the journal Agriculture, in which he was contributing as a Guest Editor [...] Full article

Esophageal squamous cell carcinoma (ESCC) is a highly prevalent malignancy worldwide. Moreover, ESCC remains poorly characterized at the molecular level, which contributes to limited therapeutic options and an overall poor prognosis. In this context, HMGA family members, which are overexpressed in tumors but almost absent in healthy adult tissues, seem to represent promising therapeutic targets. These proteins act by binding to AT-hook DNA-binding motifs and may regulate the expression of several genes associated with tumor progression. Therefore, integrating in silico, translational, and in vitro approaches, we investigated the functional consequences of blocking HMGA2–DNA interaction in ESCC tumor progression by using netropsin, a site-specific ligand for AT-rich DNA regions. Our results demonstrate that netropsin treatment significantly reduced cell viability, migration, and cell cycle progression, thereby promoting apoptosis. Furthermore, netropsin treatment was capable of partially reverting Epithelial–Mesenchymal Transition (EMT) activation associated with HMGA2 expression, by downregulating EMT activators, such as Slug and Twist. Finally, the netropsin treatment sensitizes ESCC cells to chemotherapeutic treatment with 5-Fluorouracil. Taken together, our findings highlight that AT binding-specific blockade could be correlated with the inhibition of HMGA2 and may reveal a promising approach to better understand ESCC progression. Full article

Background: Basal cell carcinoma (BCC) of the skin is the most common cancer in humans, and its incidence rises annually. Despite its low death rate, BCC causes significant morbidity because of its destructive nature to local tissues. The aim of this study was to review the state of the BCC research landscape using data published in Scopus from 1972 to 2023. Methods: Using the R package Bibliometrix, we first determined authors, countries, journals, and main topics behind the advancement of BCC research. The Latent Dirichlet Allocation (LDA), a probabilistic topic algorithm, was applied to automatically discover latent (hidden) thematic structures. Additionally, the HJ-Biplot was also chosen to visualize graphical representations of multivariate scientometric and bibliometric data to improve the LDA outcome. Results: Over five decades, we discovered 32 unique themes. BCC research has shifted from studying tumor and immunohistochemically characterization, epidemiology, and surgical/histological clearance to the advancement of diagnostic and imaging techniques, like dermoscopy and reflectance confocal microscopy (RCM). Another exciting BCC research trend has been the discovery of aberrant activation within the Hedgehog signaling. Finally, patient treatment, especially surgery, radiotherapy, topical fluorouracil, and imiquimod, is an object of intense research. Conclusions: By displaying each topic as a group of related words, this in-depth exploration outlined emerging avenues in BCC evidence-based research that will benefit from the development of cutting-edge diagnostic procedures, as well as a deeper knowledge of BCC etiology and genetic underpinnings, the quality of life in BCC patients after surgery, and the application of artificial intelligence. Full article

Leprosy presents a broad clinical–immunological spectrum, whose heterogeneity challenges early diagnosis and disease stratification. Metabolomic approaches have emerged as promising tools for identifying potential biomarkers associated with the disease’s pathophysiology. This study aimed to investigate metabolic profiles associated with the different clinical forms of leprosy using untargeted metabolomics in formalin-fixed paraffin-embedded (FFPE) tissue samples. A retrospective cross-sectional study was conducted with 55 patients classified according to the Ridley–Jopling spectrum. Metabolites were extracted from FFPE skin biopsies and analyzed by liquid chromatography–mass spectrometry (LC-MS). From 908 metabolites initially detected, 27 were retained after frequency filtering. Six metabolites ultimately met the criteria of one-way analysis of variance (ANOVA, p < 0.05) and fold-change (FC ≥ 2.0) for differential expression, while N-stearoyl tryptophan was identified as an additional candidate metabolite based on its contribution to multivariate group discrimination. These included 11-hydroperoxy-H4-neuroprostane, which showed a specific association with bacterial load, and the Gly-Pro-Lys tripeptide, which correlated with markers of infection progression. Metabolomics applied to FFPE samples proved feasible for discriminating the clinical spectrum of leprosy and annotating signatures associated with immune response. This approach represents an innovative strategy for exploratory biomarker discovery using archived histopathological samples in translational research. Full article

Roman amphorae are among the most frequently excavated archaeological ceramics and provide valuable information on ancient manufacturing techniques and trade networks. This work presents a comparative Mössbauer spectroscopy study of amphora sherds recovered from kiln sites in the Roman provinces of Hispania Lusitania and Baetica in order to reconstruct firing procedures and assess the influence of raw material composition on ceramic appearance and iron speciation. Three representative samples—São Lourenço (Lusitania), Lebrija (Baetica), and Arva (Baetica)—were investigated by ⁵⁷Fe Mössbauer spectroscopy at room temperature and 4.2 K, complemented by X-ray diffraction (XRD), X-ray fluorescence (XRF), and controlled laboratory re-firing experiments under reducing and oxidising atmospheres. The Mössbauer results reveal that all studied amphorae were originally fired under variable redox conditions, involving a reducing stage followed by partial oxidation during the final stages of firing and cooling. Re-firing experiments reproduced the original spectra and demonstrated that the observed iron phase assemblages reflect primary technological processes rather than post-depositional alteration. Low-calcium ceramics developed abundant hematite and characteristic red colours after oxidation, whereas calcium-rich ceramics favoured incorporation of iron into gehlenite and vitreous phases, producing buff or grey colouration. The Arva samples further illustrate how incomplete oxygen penetration generated red oxidised surfaces surrounding partially reduced cores. These results demonstrate that Roman amphora production at the kiln sites studied relied on empirically controlled firing regimes in open updraught kilns and that calcium content was a major factor governing both iron mineralogy and final ceramic appearance. Full article

Ecological restoration and nature repair combat ecosystem and land degradation, biodiversity loss and climate change. Yet seedling recruitment failure and perilously low plant survival (6–11%, often less) result in mass seed wastage. To increase seed use efficiency for restoration, the germination stimulant karrikinolide or KAR₁ (3-methyl-2H-furo[2,3-c]pyran-2-one) was evaluated for on-demand seed germination, seedling establishment and plant survival in Australian core restoration species. Our research demonstrated that KAR₁ promoted on-demand germination in 82% of species across nine families. We also showed improved germination outcomes in stored and aged seeds and greater seedling establishment and plant survival in soil after KAR₁ treatment. The commercially available stimulant, gibberellic acid (GA₃), provided no assistance beyond seed germination, suggesting KAR₁ cannot be readily substituted. We recommend that KAR₁ has the potential to meaningfully enhance large-scale seed use efficiency for restoration once challenges like cost and KAR₁ delivery issues are overcome. Full article

The role of the gut–brain axis is crucial in maintaining homeostasis and regulating neural, hormonal, and immunological activity. This study aimed to evaluate the effects of prebiotics or synbiotics on serum markers related to emotional disorders in individuals with morbid obesity in a triple-blind, randomized trial. The sample consisted of 22 subjects (16 women and 6 men) with a mean age of 41.8 ± 8.5 years and a mean BMI of 47.7 ± 6.8 kg/m². Serum BDNF concentrations decreased significantly after 30 days of prebiotic supplementation (p = 0.017). Serum cortisol concentrations increased in all groups between the evaluated time points, with the increase being statistically significant in the synbiotic-supplemented group (p = 0.028). Serum TNF-α concentrations increased significantly after 30 days of prebiotic supplementation when compared to the group’s baseline (p = 0.035); however, this variation did not produce a significant difference between the groups evaluated after 30 days of supplementation. The results suggest that a chronic, low-grade inflammatory state may be related to the neuroendocrine changes present in emotional disorders, but the absence of significant results in the primary outcomes is possibly due to severe underpowering. Findings from additional secondary outcomes are hypothesis-generating only, requiring confirmation in adequately powered trials. Full article

Burn injury induces profound immune dysregulation that extends beyond the acute phase of wound healing, contributing to complications such as delayed repair, infection, and long-term immune dysfunction. Importantly, these effects are not restricted to severe trauma, as similar immune alterations occur following small- to moderate-sized burns. Despite increasing recognition of post-burn immune dysregulation, targeted immunomodulatory therapies remain limited. In this review, we synthesize current insights into the mechanisms driving immune dysfunction after burn injury and outline therapeutic strategies aimed at restoring immune homeostasis. We examine approaches targeting inflammatory triggers and mediators, including acute clinical interventions, reduction in microbial burden, and inhibition of immune cell activation through systemic and local delivery. We also explore strategies to modulate dysregulated innate immune responses by targeting cell-specific functions, such as neutrophil activity and monocyte/macrophage polarization. Persistent activation and exhaustion of the adaptive immune system may be alleviated through interventions such as β-adrenergic blockade, while metabolic, endocrine, and oxidative stress pathways represent additional therapeutic targets. Finally, we highlight key challenges, including the need for improved diagnostics, early prognostic stratification, and personalized treatment approaches to improve outcomes following burn injury. Full article

Oil spills along the northeast coast of Brazil have the potential to cause catastrophic contamination of coastal environments and their associated biota. Beyond the direct contamination processes occurring on beaches, oil can also be transported inland by tides through estuaries. In addition, wind-driven transport of oil was observed in nearly all sections studied along the coast. Therefore, this study evaluated the potential of wind to transport oil fragments inland using both direct and indirect methods, including field observations and GIS-based mapping tools. The results identified and quantified oil fragmentation processes and wind-driven transport over relatively large distances (hundreds of meters). The presence of exhumed beachrock, combined with the absence or low elevation of foredunes and the high potential for wind transport, plays a crucial role in trapping oil on the beach surface. These factors further facilitate the fragmentation and inland dispersal of oil particles, allowing them to penetrate deeper into the coastal environment. The findings underscore the importance of assessing the contamination risks posed by oil fragments as they become incorporated into aeolian and other interconnected inland systems. Full article

Haemophilus influenzae b (Hib) is a pathogenic bacterium that causes meningitis worldwide, mainly in children less than two years old. The capsular polysaccharide b (PRP) is an essential antigen for vaccine formulation. This study aimed to develop a high-yield, technically accessible production strategy for PRP production to facilitate vaccine manufacturing in non-profit laboratories. Various fed-batch cultivation strategies were evaluated to address metabolic limitations and identify a robust, simplified process suitable for seamless scale-up to pilot scale. Glucose limitation strategies did not reduce inhibitory acetic acid accumulation due to deficiencies in Hib’s respiratory chain, whereas oxygen availability was identified as critical parameter. Increasing the specific air flow from 0.5 to 1.0 vvm in constant fed-batch (Cfb) resulted in a 33% yield increase, reaching 1706.40 mg PRP.L⁻¹. However, the highest PRP concentration was achieved using exponential fed-batch with cell recycling (EfbCR), resulting in 1879.28 mg PRP.L⁻¹. Although EfbCR offered high productivity, the Cfb strategy emerged to be the most technically feasible and robust solution and was successfully scaled up to an 80 L bioreactor, achieving 1885 mg PRP.L⁻¹. These results advance understanding of PRP production by Hib and provides valuable insight into an efficient and simplified strategy for producing this key/vital vaccine antigen. The findings support the potential for cost-effective local production in public health initiatives. Full article