Search Results (475)

Sustainable deepwater drilling for oil and gas offers significant potential. In this work, we synthesized a nanoscale collapse-prevention agent by grafting didecyldimethylammonium chloride onto spherical nano-silica and characterized it using Fourier-transform infrared spectroscopy, thermogravimetric analysis, zeta-potential, and particle-size measurements, as well as SEM and TEM. Adding 1 wt% of this agent to a bentonite slurry only marginally alters its rheology and maintains acceptable low-temperature flow properties. Microporous-membrane tests show filtrate passing through 200 nm pores drops to 55 mL, demonstrating excellent plugging. Core-immersion studies reveal that shale cores retain integrity with minimal spalling after prolonged exposure. Rolling recovery assays increase shale-cutting recovery to 68%. Wettability tests indicate the water contact angle rises from 17.1° to 90.1°, and capillary rise height falls by roughly 50%, reversing suction to repulsion. Together, these findings support a synergistic plugging–adsorption–hydrophobization mechanism that significantly enhances wellbore stability without compromising low-temperature rheology. This work may guide the design of high-performance collapse-prevention additives for safe, efficient deepwater drilling. Full article

Per- and polyfluoroalkyl substances (PFASs), commonly known as “forever chemicals”, are synthetic compounds with highly stable carbon–fluorine bonds, making them resistant to environmental degradation. These chemicals accumulate in ecosystems and water supplies, posing significant risks to human health, including cancer, immune system dysfunction, and neurological disorders. However, the mental health impacts of PFAS exposure remain underexplored, particularly in marginalized communities. This review examines the emerging evidence linking PFAS exposure to mental health issues such as anxiety, depression, and cognitive decline, with a focus on communities of color who face heightened vulnerability due to environmental and social health disparities. The review highlights the persistence of PFASs in the environment, common exposure pathways, and the disproportionate effects on populations living near contaminated sites. Despite some regulatory progress, U.S. regulations on PFASs are limited, especially compared to international standards. The review calls for stronger policy frameworks and emphasizes the need for environmental justice, health equity, and public awareness. By connecting environmental health, social justice, and mental well-being, the review aims to guide future research and policy reforms to mitigate the mental health consequences of PFAS exposure in vulnerable populations. Full article

Background: Elucidating the mechanisms underlying cisplatin-induced ototoxicity is critical for the clinical management of hearing loss. While cisplatin is known to penetrate the inner ear via the blood-labyrinth barrier in the stria vascularis, its precise damaging effects on marginal cells (MCs) and subsequent hearing impairment remain incompletely understood. Pyroptosis, a gasdermin-mediated inflammatory cell death pathway, may play a key role. This study investigated the involvement of gasdermin E (GSDME)-dependent pyroptosis in cisplatin-induced injury to MCs. Methods: An in vitro cisplatin-induced pyroptosis model was established in MCs. GSDME expression was downregulated using small interfering RNA (siRNA), and caspase-3 activity was inhibited pharmacologically. The critical threshold for pyroptosis induction was determined to be 5 μmol/L cisplatin exposure for 24 h, which activated the caspase-3/GSDME signaling pathway. Results: Cisplatin treatment upregulated GSDME and caspase-3 expression in MCs. Both inhibition of GSDME and pharmacological blockade of caspase-3 significantly attenuated cisplatin-induced cellular damage. Notably, caspase-3 suppression reduced GSDME expression, suggesting a positive regulatory relationship between these mediators. Conclusions: GSDME-mediated pyroptosis plays a pivotal role in cisplatin-induced marginal cell injury, with caspase-3 acting as an upstream regulator of GSDME expression. These findings provide a mechanistic foundation for developing novel therapeutic strategies against cisplatin ototoxicity. Full article

In this study, the frequency of aflatoxin M1 (AFM1) occurrence in raw milk was investigated across different seasons over a three-year period from 2022 to 2024 in Croatia. Risk assessment was conducted using estimated daily intake (EDI), hazard index (HI), and margin of exposure (MOE) for various age groups and both genders. The frequency of AFM1 detection above the maximum level (ML) ranged from 1.60% to 15.1%. The average incidence of AFM1 exceeding the ML was 5.67%, with the highest incidence recorded in autumn 2024. AFM1 levels within the limit of detection (LOD) and ML were found in 13% of the samples. The average mean value of AFM1 over the three-year period was 19.2 ng/kg. The highest mean AFM1 EDI values were determined for toddlers (0.61–0.67 ng/kg bw/day) and children (0.41–0.43 ng/kg bw/day). The lowest EDI values were observed in elderly females and males (0.058–0.074 ng/kg bw/day). The EDI values for females and males were slightly different. The risk assessment, based on the HI and MOE, indicated that toddlers and children are at the highest risk of exposure to AFM1, which raises significant health concerns. Additionally, consumers of large quantities of milk face a high risk of exposure, particularly during the spring and autumn seasons. For adults and the elderly, milk consumption does not pose a serious health risk. Full article

Tropical montane cloud forests (TMCFs) are biodiversity hotspots that have been increasingly cleared to cultivate coffee under full sun exposure, replacing traditional shaded agroforestry systems. This study evaluated the impact of TMCF clearing on soil quality by analyzing 108 samples from undisturbed primary and secondary forests and deforested coffee plantations in the Dominican Republic and Haiti. Our findings indicate that forest clearing has a substantial adverse impact on soil nutrient status. Soils from undisturbed plots had total organic carbon (TOC) concentrations 4.83 units higher than those from cleared plots. Nitrogen levels were reduced by 28–61%, and available potassium declined by 23–51% in soils that had been cleared. Conversely, the available phosphorus levels exhibited a modest increase (ranging from 23% to 27%) following the clearing process, presumably attributable to diminished plant uptake and augmented mineralization in conditions characterized by diminished organic matter. However, given that phosphorus is not a limiting factor for coffee growth, this marginal gain does not compensate for the broader degradation of soil fertility. The study emphasizes that allowing TMCFs to be used for sun-grown coffee results in long-term nutrient depletion through erosion and leaching, which poses a threat to both the productivity of the soil and the ecological integrity of these valuable forest systems. Full article

This study investigated how corporate maturity—measured through firm age and lifecycle stage—moderates the value relevance of Environmental, Social, and Governance (ESG) disclosures in a frontier market context, using Bangladesh as a case study. Drawing on panel data from 2011–2012 to 2023–2024 for 86 publicly listed non-financial firms, the study employed a modified Ohlson valuation framework, panel regression analysis, and multiple robustness techniques (2SLS, PSM). ESG disclosure was measured using a researcher-developed index aligned with international reporting standards (GRI, SASB, TCFD, UN SDGs). ESG disclosures are positively associated with firm value, but this relationship is significantly moderated by corporate maturity. Younger firms exhibit a stronger valuation effect from ESG transparency, driven by higher signaling and legitimacy needs. In contrast, mature firms experience a diminished marginal benefit, reflecting routine compliance rather than strategic differentiation. These findings challenge the uniform application of ESG assessment models and suggest the need for lifecycle-adjusted disclosure ratings, particularly in nascent regulatory environments like Bangladesh. Investors and regulators should tailor ESG evaluation criteria by firm age and industry sustainability exposure. Younger firms, often overlooked, may carry outsized ESG signaling value in emerging markets. Enhancing ESG transparency among younger firms can foster greater stakeholder trust, support inclusive growth, and strengthen social accountability in emerging economies. This study contributes to the ESG literature by introducing corporate maturity as a key moderating variable in value relevance analysis. It provides new empirical insights from a developing economy and proposes lifecycle-based adaptations to global ESG rating methodologies. Full article

Dietary exposure of Singapore population to foodborne and natural toxins was estimated through Total Diet Study (TDS) approach. Among the common mycotoxins and plant toxins studied, such as aflatoxins, ochratoxin A, zearalenone, deoxynivalenol, and fumonisins, aflatoxins were identified with food safety concerns. Aflatoxin occurrence was determined in 642 commonly consumed foods, with a detection rate of 4%, and a mean concentration of 0.01–0.07 µg/kg. Dietary exposure and risk assessment of aflatoxins for the general population revealed a mean estimated daily intake (EDI) of 0.0002–0.002 ng/kg bw/day, a margin of exposure (MOE) of 2819–7101, cancer risk of 0.002–0.004 additional cases per 100,000 person per year, and a hazard quotient (HQ) of 0.19–0.20. Despite the low overall estimated exposure risk for the general population, elevated exposure was observed among the eaters-only group, with the highest upper-bound (UB) exposure reaching 3.4 ng/kg bw/day for high consumers (95th percentile) of satay sauce, a popular Asian delicacy. The corresponding cancer risk of 0.23 additional cases per 100,000 individuals, or 14 additional cases annually, contributes to an estimation of 1% of the 1442 liver cancer cases reported in Singapore in 2022. These findings highlight the importance of continuous monitoring and call for appropriate mitigation strategies for further reduction in aflatoxin exposure in the Singapore population. Full article

Arsenic is a human carcinogen present in drinking water and food, especially rice, rice products and seafood. It can be found in both organic and inorganic forms, the latter being the most toxic. In addition to the carcinogenic effect, exposure to inorganic arsenic can cause numerous disorders in different organs/systems of the human body, such as the skin, cardiovascular, neurological, endocrine, immune, and reproductive systems. The risk assessment associated with dietary arsenic is mainly based on the margin of exposure, i.e., the ratio between the dose at which a small but measurable adverse effect may occur and the estimated daily intake of the target substance. It is mainly influenced by arsenic concentrations and consumption data of average or 95th percentile consumers. This review focuses on the toxicity of arsenic, its sources and routes of human exposure, with particular attention to the ingestion of contaminated water and food, considering the differences between age groups and dietary habits. Full article

This study evaluated the prevalence of aflatoxin M1 (AFM1) in milk marketed in Tirana, Albania, along with dietary exposure and associated potential health risks. The World Health Organization has categorized Albania in cluster G02 of GEMS/FOOD, highlighting that milk is a staple in the Albanian diet, which points to a possible health risk. A total of 141 milk samples, comprising both Ultra-High Temperature (UHT) and pasteurized types, were collected from local markets in Tirana and analyzed from March 2023 to February 2024. The determination of AFM1 levels was carried out using High-Pressure Liquid Chromatography with a Fluorescence Detector (HPLC-FLD), a precise and dependable technique for identifying and measuring aflatoxins in food products. Aflatoxin M1 was found in 62.4% of the milk samples, with 26.2% surpassing the European Union’s maximum residue levels (MRL). The mean AFM1 concentrations were 58.8 ± 95.8 ng/kg, reaching a maximum level of 399.0 ng/kg. The Estimated Daily Intake (EDI) for various groups—toddlers, children, adolescents, and adults—was determined to be 2.161, 1.297, 0.519, and 0.370 ng/kg of body weight per day, respectively. The Hazard Index (HI), derived from the AFM1 exposure for four population groups, was 10.81 (toddlers), 6.48 (children), 2.59 (adolescents), and 1.85 (adults). The Margin of Exposure (MoE) was 1.85, 3.08, 7.71, and 10.81, respectively. The incidence of hepatocellular carcinoma (HCC) per 100,000 people in the four groups was 0.034, 0.021, 0.008, and 0.006, respectively. The study is the first comprehensive evaluation of AFM1 prevalence, highlighting the potential risks associated with milk consumption, as milk is a dietary staple in Albanian households. It addresses a critical public health concern regarding aflatoxin M1 (AFM1) contamination in milk consumed in Tirana, Albania, by highlighting the need for ongoing monitoring, regulatory measures, and educational outreach to enhance food safety and safeguard public health in Albania, as well as in other regions facing similar concerns. Full article

The aim of this article is to forecast the systemic risk contribution and exposure measured by the delta conditional value at risk (ΔCoVaR) and the marginal expected shortfall (MES), respectively. We first estimate the ΔCoVaR and MES for banks in 16 European countries for the 2002–2016 period. We then predict systemic risk measures using machine learning techniques, such as artificial neural network (ANN) and support vector machine (SVM), and we use AR-GARCH specification. Finally, we compare the methods’ forecasting values and the actual values. Our results show that two hidden layers of artificial neural networks perform efficiently in forecasting systemic risk. Full article

Background: Autism spectrum disorder (ASD) is increasingly linked to systemic metabolic dysfunction, potentially influenced by gut–brain axis dysregulation, but the underlying mechanisms remain unclear. Methods: We developed Personalized Metabolic Margin Mapping (PM³), a computational systems biology framework, to analyze RNA-seq data from 12 ASD and 12 control postmortem brain samples. The model focused on 158 curated metabolic genes selected for their roles in redox balance, mitochondrial function, neurodevelopment, and gut–brain interactions. Results: Using unsupervised machine learning (Isolation Forest) to detect outlier expression patterns, Euclidean distance, and percent expression difference metrics, PM³ revealed a consistent downregulation of glycolysis (e.g., −5.4% in PFKM) and mitochondrial enzymes (e.g., −12% in SUCLA2). By incorporating cofactor dependency and subcellular localization, PM³ identified a coordinated suppression of multivitamin transporters (e.g., −4.5% in SLC5A6, −3.5% in SLC19A2), potentially limiting cofactor availability and compounding energy deficits in ASD brains. Conclusions: These findings suggest a convergent metabolic dysregulation signature in ASD; wherein the subtle suppression of cofactor-dependent pathways may impair energy metabolism and neurodevelopment. We propose that chronic microbial lipopolysaccharide (LPS) exposure in ASD suppresses vitamin transporter function, initiating mitochondrial dysfunction and transcriptomic reprogramming. Validation in LPS-exposed systems using integrated transcriptomic–metabolomic analysis is warranted. Full article

This study integrates socio-demographic factors and local sustainability indicators to assess their implications for public health and social vulnerability in the Henequén neighborhood of Cartagena, Colombia. This historically marginalized community, primarily composed of women and displaced families, faces chronic exposure to environmental contaminants due to its past as a municipal landfill. Poor housing conditions, overcrowding, and inadequate access to water and sanitation services exacerbate health risks. Additionally, low educational attainment and limited economic opportunities contribute to cycles of poverty and illicit activities, disproportionately affecting children’s development. Using a cross-sectional and correlational approach, the study identifies key variables, such as housing conditions, access to basic services, and marital status, that shape social vulnerability. The findings are analyzed in the context of the United Nations Sustainable Development Goals (SDGs), particularly SDG 3 (Good Health and Well-being), SDG 6 (Clean Water and Sanitation), and SDG 11 (Sustainable Cities and Communities). The study highlights critical gaps in sustainability efforts and provides a framework for assessing local progress toward achieving these global development objectives. Full article

Floating aquatic vegetation and algal blooms are increasing with global warming, potentially reducing UVB exposure and, consequently, vitamin D (vit-D) synthesis in freshwater turtles. Vit-D mediates calcium metabolism and overall health, yet the effects of floating aquatic vegetation on vit-D levels remain unclear, as is whether turtles actively avoid habitats with abundant floating vegetation. Here, we address these questions by quantifying vit-D₃ levels in the blood of adult female painted turtles (Chrysemys picta) exposed to high-vegetation (darker/colder) or clear-water (lighter/warmer) treatments for one month outdoors and one month indoors at a single temperature during late summer and early fall. The observed circulating vit-D₃ levels resembled those reported for other freshwater turtles, declined over time in both treatments, and were marginally lower under high vegetation after 60 days compared to clear water. However, this difference disappeared after correcting for lymph contamination and multiple comparisons, suggesting that perhaps adult females are robust to the effect of floating vegetation, but whether they were buffered by vit-D₃ stores in lipids is unclear. Additionally, in subsequent years, females were exposed to habitat choice experiments and exhibited a strong preference for high floating vegetation over clear water, both as a group (outdoors) and individually (outdoors, and indoors at 21 °C and 26 °C), consistent with known benefits conferred by floating vegetation (food, predator avoidance). While no ill effects of high vegetation nor behavioral avoidance were detected here, longer experiments at different seasons on both sexes and varying ages are warranted before concluding whether painted turtles are truly resilient in their vit-D levels or if, instead, a tradeoff exists between the known benefits of floating vegetation and potential [yet unidentified] detrimental effects (lower dissolved oxygen or vit-D) when vegetation is overgrown for extended periods. Full article

This study is the first to scrutinize the rates of, and the lexical diversity in, adjective intensification in second language (L2) German. We additionally attend to the issue concerning whether sociodemographic variables (i.e., length of residence, age, and gender) and individual learner differences (i.e., L2 proficiency, intensity of exposure to the L2, and L2 socioaffect) can predict (a) the inter-individual variation in syntactic adjective intensification, and (b) the observed intra-individual variation based on a weighted measure of intensifier lexical diversity. We analyzed spoken data collected via virtual reality (VR) elicitation tasks from 40 learners of L2 German (first language [L1] English). We found that learners engaged in adjective intensification at similar rates as those reported in the literature, despite some cases of overshooting the target; learners also preferred markers of intensification consistent with the lexical choices of L1 German speakers. Sociodemographic variables did not predict different rates of adjective intensification; rather, individual learner differences such as those relating to L2 proficiency and L2 exposure correlated with more target-like use of intensifiers, though the correlations were weak. The diversity in adjective intensification was also only marginally related to demographic factors and individual learner differences. Our findings suggest that L2 learners indeed engage in similar intensification practices as do L1 speakers; however, systematically predicting more ‘successful’ adoption of target-like sociopragmatic norms among L2 learners remains challenging. Full article

Fruit spirits have roots in traditions across Eastern and Central Europe. Homemade/unrecorded spirits are typically produced under inconsistent conditions, leading to inconsistent product quality. From the safety aspect, great concentration variability of exclusively harmful substances (methanol and acetaldehyde) and compounds with either favorable or hazardous properties (ethyl acetate and higher alcohols), depending on their amount, must be considered. An option to ensure their safety could be a dephlegmator, a central component in column distillation systems. To determine whether such an approach in small-scale spirit production lessens the health risks from harmful volatile compounds, 35 fruit spirits were investigated using HSS-GC-FID. Dephlegmator usage was associated with lower median methanol concentrations (1878 vs. 3723 mg/L p.a.) and a narrower concentration span. The remaining analytes showed no significant reduction in median level; however, the ranges have narrowed. A risk assessment (margin of exposure approach) revealed that dephlegmator usage increased the proportion of methanol-safe samples. The risk of acetaldehyde was equivalent or somewhat greater in the dephlegmator spirit group, suggesting challenges for optimizing the head cut during distillation. Ethyl acetate and higher alcohols did not pose a risk regardless of dephlegmator use. These findings support dephlegmator usage as a useful but insufficient intervention in home/small-scale spirit production to obtain safer products of consistent quality. Full article