Search Results (40)

Maintenance of building fire protection facilities is crucial for preventing fires and safeguarding lives and property; the standardization and timeliness of these activities directly determine operational reliability. However, as fire-safety requirements escalate, manually drafting maintenance work orders remains inefficient and prone to omissions. Furthermore, regulatory documents in this domain are inherently complex, and annotated resources are scarce, hampering the digitalization of fire-safety management. To address these challenges, this paper presents an LLM-based method for automatically generating maintenance work orders for building fire protection facilities. The proposed approach integrates a domain-specific knowledge base and incorporates the FS-RAG (Fire Services–Retrieval-Augmented Generation) framework to enhance both the accuracy and practical usability of generated work orders. First, we construct a lightweight domain knowledge base, FSKB (Fire Services Knowledge Base), derived from extensive maintenance regulations, capturing key elements such as equipment types, components, maintenance actions, and frequencies. Second, we design an FS-RAG framework that leverages retrieval-augmented generation to extract critical information from regulations and fuse it with the knowledge base, ensuring high accuracy and operational feasibility. Multi-round evaluations across stages B0–B4 validate the effectiveness of our method. Results indicate significant improvements over traditional approaches: the line-level compliance rate reaches 97.3% (an increase of 5.7% over B1 and 30.4% over B0), and the F1 score achieves 90.42% (an increase of 12.62% over B1 and 29.87% over B0). Full article

Overhead transmission lines often suffer from mutual collisions between adjacent conductors in windy weather, which can cause power failures to villages. To solve this problem, this paper introduces a spacer ring and a teleoperated robot for the installation and retrieval of the ring. The spacer ring and robot address the installation challenges of the anti-collision devices and enhance transmission line maintenance. Fixed by the locking mechanism, the spacer ring can isolate adjacent conductors to avoid collisions. The structure and working principle of the spacer ring and installation robot are introduced. Static analysis and finite element analysis (FEA) are conducted to analyze the output force of the locking mechanism, which is then validated through experiments. Experimental results show that the locking mechanism can generate a strong output force of up to 2000 N with about 6.0 N·m of input torque, providing a secure installation for the spacer ring. Diverse installation tests have validated the robot’s capability for live-line operations on transmission lines. Field tests indicate that the installation robot can travel at 0.3 m/s on a 15° slope and successfully install the spacer rings. Full article

As the core component of electrified railway power supply systems, the fatigue performance and reliability of catenary support structures are directly related to the operational safety of high-speed railways. To address the problem of structural fatigue damage caused by increasing train speed and high-frequency operation, this study develops a refined finite element model including a support structure, suspension system and support column, and the dynamic response characteristics and fatigue life evolution law under train operation conditions are systematically analyzed. The results show that under the conditions of 250 km/h speed and 100 times daily traffic, the fatigue lives of the limit locator and positioning support are 43.56 years and 34.48 years, respectively, whereas the transverse cantilever connection and inclined cantilever have infinite life characteristics. When the train speed increases to 400 km/h, the annual fatigue damage of the positioning bearing increases from 0.029 to 0.065, and the service life is shortened by 55.7% to 15.27 years, which proves that high-speed working conditions significantly aggravate the deterioration of fatigue in the structure. The reliability analysis based on Monte Carlo simulation reveals that when the speed is 400 km/h and the daily traffic is 130 times, the structural reliability shows an exponential declining trend with increasing service life. If the daily traffic frequency exceeds 130, the 15-year reliability decreases to 92.5%, the 20-year reliability suddenly decreases to 82.4%, and there is a significant inflection point of failure in the 15–20 years of service. Considering the coupling effect of environmental factors (wind load, temperature and freezing), the actual failure risk may be higher than the theoretical value. On the basis of these findings, engineering suggestions are proposed: for high-speed lines with a daily traffic frequency of more than 130 times, shortening the overhaul cycle of the catenary support structure to 7–10 years and strengthening the periodic inspection and maintenance of positioning support and limit locators are recommended. The research results provide a theoretical basis for the safety assessment and maintenance decision making of high-speed railway catenary systems. Full article

Background: During infection with the cytomegalovirus (CMV), the membrane system of the infected cell is remodelled into a megastructure called the assembly compartment (AC). These extensive changes may involve the manipulation of the host cell proteome by targeting a pleiotropic function of the cell such as ubiquitination (Ub). In this study, we investigate whether the Ub system is required for the establishment and maintenance of the AC in murine CMV (MCMV)-infected cells Methods: NIH3T3 cells were infected with wild-type and recombinant MCMVs and the Ub system was inhibited with PYR-41. The expression of viral and host cell proteins was analyzed by Western blot. AC formation was monitored by immunofluorescence with confocal imaging and long-term live imaging as the dislocation of the Golgi and expansion of Rab10-positive tubular membranes (Rab10 TMs). A cell line with inducible expression of hemagglutinin (HA)-Ub was constructed to monitor ubiquitination. siRNA was used to deplete host cell factors. Infectious virion production was monitored using the plaque assay. Results: The Ub system is required for the establishment of the infection, progression of the replication cycle, viral gene expression and production of infectious virions. The Ub system also regulates the establishment and maintenance of the AC, including the expansion of Rab10 TMs. Increased ubiquitination of WASHC1, which is recruited to the machinery that drives the growth of Rab10 TMs, is consistent with Ub-dependent rheostatic control of membrane tubulation and the continued expansion of Rab10 TMs. Conclusions: The Ub system is intensively utilized at all stages of the MCMV replication cycle, including the reorganization of the membrane system into the AC. Disruption of rheostatic control of the membrane tubulation by ubiquitination and expansion of Rab10 TREs within the AC may contribute to the development of a sufficient amount of tubular membranes for virion envelopment. Full article

The use of high-pressure grinding rolls (HPGRs) is increasing in the ore industries as advanced technology is available for this type of comminuting. There are important parameters in these devices, which have many effects on productivity. One of the main reasons for damage on the rolls and, therefore, decreases in the machine’s productivity and efficiency is surface wear. This phenomenon must be carefully understood so that it can be controlled as much as possible through the readjustment and optimization of the effective parameters. In this research, the wear mechanism of HPGRs in a production line for iron ore concentrate was investigated. The results showed that there was greater wear at the center of the rolls and that changes to the chemical and physical properties of the incoming iron compared to the design condition reduced the rolls’ lives. The results showed a failure to perform appropriate mechanical adjustment and improper repair and maintenance. Full article

Adopting mobile robots for high voltage (HV) live-line operations can mitigate personnel casualties and enhance operational efficiency. However, conventional mechanical arms cannot inspect concealed spaces in the power cable tunnel because their joint integrates metallic motors or hydraulic serial-drive mechanisms, which limit the arm’s length and insulation performance. Therefore, this study proposes a 7-degree-of-freedom (7-DOF) bionic mechanical arm with rigid-flexible coupling, mimicking human arm joints (shoulder, elbow, and wrist) designed for HV live-line operations in concealed cable tunnels. The arm employs a tendon-driven mechanism to remotely actuate joints, analogous to human musculoskeletal dynamics, thereby physically isolating conductive components (e.g., motors) from the mechanical arm. The arm’s structure utilizes dielectric materials and insulation-optimized geometries to reduce peak electric field intensity and increase creepage distance, achieving intrinsic self-insulation. Furthermore, the mechanical design addresses challenges posed by concealed spaces (e.g., shield tunnels and multi-circuit cable layouts) through the analysis of joint kinematics, drive mechanisms, and dielectric performance. The workspace of the proposed arm is an oblate ellipsoid with minor and major axes measuring 1.25 m and 1.65 m, respectively, covering the concealed space in the cable tunnel, while the arm’s quality is 4.7 kg. The maximum electric field intensity is 74.3 kV/m under 220 kV operating voltage. The field value is less than the air breakdown threshold. The proposed mechanical arm design significantly improves spatial adaptability, operational efficiency, and reliability in HV live-line inspection, offering theoretical and practical advancements for intelligent maintenance in cable tunnel environments. Full article

This article covers the analysis of voltages induced on the conductors of a de-energized circuit of a multi-circuit, multi-voltage HVAC transmission line. As a result of the multiplied interactions between the circuits in such lines, the expected electrical shock currents (touch currents) to which a lineman performing live work on such a line may be exposed are determined. A number of supporting structures of three- and four-circuit lines with various degrees of geometric asymmetry are analyzed. Analyses have shown that in multi-circuit lines in which circuits of different voltages are carried on a common structure, despite the outage of one of the circuits, touch voltages and electrical shock currents (touch currents) exceeding the permissible values can be expected on its conductors, endangering the safety of the lineman. The arrangements of s in such lines that provide the smallest values of touch currents are indicated. Full article

To provide diverse in-home services like elderly care, versatile activity recognition technology is essential. Radio-based methods, including WiFi CSI, RFID, and backscatter communication, are preferred due to their minimal privacy intrusion, reduced physical burden, and low maintenance costs. However, these methods face challenges, including environmental dependence, proximity limitations between the device and the user, and untested accuracy amidst various radio obstacles such as furniture, appliances, walls, and other radio waves. In this paper, we propose a frequency-shift backscatter tag-based in-home activity recognition method and test its feasibility in a near-real residential setting. Consisting of simple components such as antennas and switches, these tags facilitate ultra-low power consumption and demonstrate robustness against environmental noise because a context corresponding to a tag can be obtained by only observing frequency shifts. We implemented a sensing system consisting of SD-WiFi, a software-defined WiFi AP, and physical switches on backscatter tags tailored for detecting the movements of daily objects. Our experiments demonstrate that frequency shifts by tags can be detected within a 2 m range with 72% accuracy under the line of sight (LoS) conditions and achieve a 96.0% accuracy (F-score) in recognizing seven typical daily living activities with an appropriate receiver/transmitter layout. Furthermore, in an additional experiment, we confirmed that increasing the number of overlaying packets enables frequency shift-detection even without LoS at distances of 3–5 m. Full article

Hordeum vulgare genes NUD (HvNUD) and WIN1 (HvWIN1) play a regulatory role in cuticle organization. Because the cuticle is a key evolutionary acquisition of plants for protection against environmental factors, a knockout (KO) of each gene may alter their ability to adapt to unfavorable conditions. A potential pleiotropic effect of HvNUD or HvWIN1 gene mutations can be assessed under salt stress. Initial developmental stages are the most sensitive in living organisms; therefore, we evaluated salt tolerance of nud KO and win1 KO barley lines at the seedling stage. Air-dried barley grains of the KO lines and of a wild-type (WT) line were germinated in NaCl solutions (50, 100, or 150 mM). Over 30 physiological and morphological parameters of seedlings were assessed. Potential pleiotropic effects of the HvNUD gene KO under salt stress included the stimulation of root growth (which was lower under control conditions) and root necrosis. The pleiotropic effects of the HvWIN1 gene KO under the stressful conditions manifested themselves as maintenance of longer root length as compared to the other lines; stable variation of most of morphological parameters; lack of correlation between root lengths before and after exposure to NaCl solutions, as well as between shoot lengths; and the appearance of twins. Salt tolerance of the analyzed barley lines could be ranked as follows: nud KO > win1 KO ≈ WT, where nud KO lines were the most salt-tolerant. A comparison of effects of salinity and ionizing radiation on nud KO and win1 KO barley lines indicated differences in tolerance of the lines to these stressors. Full article

Glioblastoma (GBM) is the most malignant form of primary brain tumor. It is characterized by the presence of highly invasive cancer cells infiltrating the brain by hijacking neuronal mechanisms and interacting with non-neuronal cell types, such as astrocytes and endothelial cells. To enter the interstitial space of the brain parenchyma, GBM cells significantly shrink their volume and extend the invadopodia and lamellipodia by modulating their membrane conductance repertoire. However, the changes in the compartment-specific ionic dynamics involved in this process are still not fully understood. Here, using noninvasive perforated patch-clamp and live imaging approaches on various GBM cell lines during a wound-healing assay, we demonstrate that the sodium-calcium exchanger (NCX) is highly expressed in the lamellipodia compartment, is functionally active during GBM cell migration, and correlates with the overexpression of large conductance K+ channel (BK) potassium channels. Furthermore, a NCX blockade impairs lamellipodia formation and maintenance, as well as GBM cell migration. In conclusion, the functional expression of the NCX in the lamellipodia of GBM cells at the migrating front is a conditio sine qua non for the invasion strategy of these malignant cells and thus represents a potential target for brain tumor treatment. Full article

The genitourinary microbiome plays a crucial role in the establishment and maintenance of urinary and reproductive health in women throughout their lives. Particularly during the reproductive stage, resident microorganisms contribute to implantation and protect against perinatal complications, including preterm birth, stillbirth, and low birth weight, while also serving as the first line of defense against pathogens that can cause infections, such as urinary tract infections and bacterial vaginosis. This review aimed to elucidate the relationship between a healthy microbiome environment and women’s overall health. We examine the variability and dynamics of the microbiome during different developmental stages, ranging from the prepubertal to the postmenopausal stage. Furthermore, we explore the significance of a healthy microbiota in successful implantation and pregnancy development and investigate potential differences between women experiencing infertility. In addition, we analyze the local and systemic inflammatory responses associated with the establishment of a dysbiotic state and compare it to a condition where a healthy microbiome was established. Lastly, we present the most recent evidence regarding preventive measures, such as dietary interventions and the use of probiotics to promote and maintain a healthy microbiome, thereby ensuring comprehensive women’s health. By highlighting the importance of the genitourinary microbiome in reproductive health, this review aimed to enhance this microbiome’s visibility and significance in the field. Full article

A three-dimensional (3D) lung aggregate model based on sodium alginate scaffolds was developed to study the interactions between Paracoccidioides brasiliensis (Pb) and lung epithelial cells. The suitability of the 3D aggregate as an infection model was examined using cell viability (cytotoxicity), metabolic activity, and proliferation assays. Several studies exemplify the similarity between 3D cell cultures and living organisms, which can generate complementary data due to the greater complexity observed in these designed models, compared to 2D cell cultures. A 3D cell culture system of human A549 lung cell line plus sodium alginate was used to create the scaffolds that were infected with Pb18. Our results showed low cytotoxicity, evidence of increased cell density (indicative of cell proliferation), and the maintenance of cell viability for seven days. The confocal analysis revealed viable yeast within the 3D scaffold, as demonstrated in the solid BHI Agar medium cultivation. Moreover, when ECM proteins were added to the alginate scaffolds, the number of retrieved fungi was significantly higher. Our results highlight that this 3D model may be promising for in vitro studies of host–pathogen interactions. Full article

Tinnitus is a multifactorial phenomenon and psychological, audiological, or medical factors can facilitate its onset or maintenance. A growing body of research investigates individuals’ perceptions, associations, and experiences of living with tinnitus. This body of research examines tinnitus as a condition rather than a symptom. We examine a sample of chronic tinnitus patients in terms of associations that are induced by neutral sounds. In particular, we investigate how patients with chronic tinnitus ascribe meaning to those neutral sounds. The present study uses Mayring’s content analysis to explore the content of psychological associations underlying valence ratings of everyday neutral sounds. Nine tinnitus patients completed a hearing exercise, during which they listened to seven neutral sounds, following which we examined their sound-induced associations using semi-structured interviews. Three groups of factors influenced patients’ associations and valence ratings of neutral sounds: affect, episodic memory, and ‘other’. The former two factors further comprised two subcategories. In line with previous psychoaudiological research designs, our findings suggest that neutral, everyday auditory stimuli evoke strong affective reactions—possibly through serving as retrieval cues for episodic memories. Based on these findings, we discuss our results in the context of previous psychoaudiological findings and propose further research concerning psychological associations that may specifically underlie the tinnitus sound. Full article

A 10 kV distribution network is a crucial piece of infrastructure to guarantee enterprises’ and households’ access to electricity. Stripping cables is one of many power grid maintenance procedures that are now quickly expanding. However, typical cable-stripping procedures are manual and harmful to workers. Although numerous automated solutions for grid maintenance have been created, none of them focus on cable stripping, and most of them have large dimensions to guarantee multi-functions. In this paper, a new cable-stripping robot for the 10 kV power system is introduced. The design of a live working cable-stripping robot that is appropriate for installing insulating rods is introduced, taking into account the working environment of 10 kV overhead lines and the structural characteristics of overhead cables. The robot is managed by an auxiliary remote control device. A cascade PID control technology based on the back propagation neural network (BPNN) method was developed, as the stripper robot’s whole system is nonlinear and the traditional PID controller lacked robustness and adaptability in complex circumstances. To validate the structural feasibility of the cable-stripping robot, as well as the working stability and adaptability of the BPNN–PID controller, a 95 mm² cable-stripping experiment are carried out. A comparison of the BPNN–PID controller with the traditional PID method revealed that the BPNN–PID controller has a greater capacity for speed tracking and system stability. This robot demonstrated its ability to replace manual stripping procedures and will be used for practical routine power maintenance. Full article

Throughout the animal kingdom, our two chemical senses, olfaction and gustation, are defined by two primary factors: genomic architecture of the organisms and their living environment. During the past three years of the global COVID-19 pandemic, these two sensory modalities have drawn much attention at the basic science and clinical levels because of the strong association of olfactory and gustatory dysfunction with viral infection. Loss of our sense of smell alone, or together with a loss of taste, has emerged as a reliable indicator of COVID-19 infection. Previously, similar dysfunctions have been detected in a large cohort of patients with chronic conditions. The research focus remains on understanding the persistence of olfactory and gustatory disturbances in the post-infection phase, especially in cases with long-term effect of infection (long COVID). Also, both sensory modalities show consistent age-related decline in studies aimed to understand the pathology of neurodegenerative conditions. Some studies using classical model organisms show an impact on neural structure and behavior in offspring as an outcome of parental olfactory experience. The methylation status of specific odorant receptors, activated in parents, is passed on to the offspring. Furthermore, experimental evidence indicates an inverse correlation of gustatory and olfactory abilities with obesity. Such diverse lines of evidence emerging from basic and clinical research studies indicate a complex interplay of genetic factors, evolutionary forces, and epigenetic alterations. Environmental factors that regulate gustation and olfaction could induce epigenetic modulation. However, in turn, such modulation leads to variable effects depending on genetic makeup and physiological status. Therefore, a layered regulatory hierarchy remains active and is passed on to multiple generations. In the present review, we attempt to understand the experimental evidence that indicates variable regulatory mechanisms through multilayered and cross-reacting pathways. Our analytical approach will add to enhancement of prevailing therapeutic interventions and bring to the forefront the significance of chemosensory modalities for the evaluation and maintenance of long-term health. Full article