Search Results (413)

We report on X-ray-induced Sm³⁺ → Sm²⁺ reduction in SrF₂:Sm³⁺ nanocrystals of ~40 nm size synthesized via a co-precipitation method. Non-irradiated samples show characteristic Sm³⁺ f-f ⁴G_5/2 → ⁶H_5/2, ⁶H_7/2, ⁶H_9/2, and ⁶H_11/2 emissions, while X-irradiation induces intense low-temperature Sm^{2+ 5}D₀ → ⁷F₁ emission and other Sm²⁺ lines. The evolution of Sm³⁺ and Sm²⁺ photoluminescence intensities with X-ray dose (0–300 Gy) follows first-order kinetics, consistent with a trapping–detrapping mechanism. Compared to CaF₂:Sm³⁺, SrF₂:Sm³⁺ exhibits faster Sm³⁺ reduction due to the higher X-ray absorption cross section of strontium compared to calcium for Cu-Kα (8 keV) radiation, highlighting its potential as a nanoscale X-ray storage phosphor. Full article

Precise manipulation of two-phase flow in micro-confined electrowetting pixels is limited by contact angle hysteresis (CAH). To elucidate this non-equilibrium process, we establish a high-fidelity electrohydrodynamic (EHD) phase-field simulation framework. The model rigorously couples Navier–Stokes equations with molecular kinetic theory (MKT) to characterize energy dissipation at the three-phase contact line (TCL) and further integrates charge transport kinetics. Numerical results reveal CAH is driven by physical pinning and interfacial charge trapping, with the latter dominating interfacial retreat and causing significant residual displacement. Furthermore, analysis shows alternating current (AC) waveforms mitigate charge accumulation and promote depinning via micro-oscillations, minimizing the hysteresis loop compared to direct current (DC) waveforms. Additionally, an overdrive strategy utilizing a suprathreshold Maxwell stress pulse rapidly overcomes static friction. This strategy significantly improves transient dynamics, substantially reducing the time to reach 90% of the steady-state target from 19.6 ms (under standard DC waveform driving) to 7.4 ms. This work provides a comprehensive theoretical basis and design criteria for optimizing active driving strategies in optofluidic and digital microfluidic systems. Full article

Recent years have witnessed growing interest in CO₂ and in the possibility of injecting it into the Earth’s crust for multiple purposes. In addition to the fact that pure CO₂ is already present in some geological formations, the most debated is Carbon Capture and Storage (CCS), which aims to capture and trap CO₂ through water-assisted reactions that promote its precipitation; moreover, proposed technological improvements to geothermal plants foresee the use of pure CO₂ as a working fluid and energy carrier for electricity generation in terms of MWh. These applications require detailed knowledge and a deep understanding of CO₂ behaviour under non-standard conditions. Upon entering the Earth’s crust, CO₂ is subjected to progressively increasing temperature and pressure. The resulting effects are not limited to a reduction in intermolecular distance; they also include changes in molecular geometry, as well as in chemical and thermodynamic behaviour. For instance, a dipole moment may arise even in the gaseous phase as intermolecular distances decrease. Moreover, CO₂ typically reaches supercritical conditions at depths of approximately 700 m. It is therefore necessary to account for both phase transitions and variations in molecular structure, as these can significantly influence the surrounding environment and the stoichiometric relationships with other substances. In this work, a steady-state column was simulated, representing CO₂ injection down to a depth of 5 km, assuming an average geothermal gradient of 30 °C/km and nine different initial pressures, so nine different steady state columns. The results highlight the presence of a wedge-shaped region acting as a barrier for stepwise-equilibrated CO₂: the computed CO₂ column profiles avoid this region. This wedge includes part of the liquid–gas boundary under subcritical conditions, as well as the Widom lines above the critical point. It effectively separates two supercritical regimes, namely gas-like and liquid-like domains. In this context, the present work provides insights into the Widom region—possibly extending into subcritical conditions—and into these two distinct regimes. This may have implications for the solvent capacity of CO₂ for ionic species. Ultimately, the initial pressure appears to determine the behaviour of CO₂ at depth. Full article

TiO₂ films with a thickness of 20 nm were obtained by anodizing a titanium film with an aluminum sublayer on a glass substrate. The I–V characteristics were studied in a temperature range of 100–300 K. Three linear sections can be distinguished on the I–V curves in logarithmic coordinates with a bias voltage of up to 2.5 V. The first section is an ohmic section with a bias voltage sweep from 0 V. The second section is associated with the space-charge-limited currents. The third section is characterized by the flow of Poole–Frenkel currents. In the third section, the slope of the approximating line is greater than in the second one due to the flow of higher currents. This is explained by the transition of electrons from donor centers to trap levels, which leads to a decrease in the number of free traps available for capturing electrons injected from the contacts into the conduction band. The obtained values of the Fermi energy of 0.032 and 0.028 eV for temperatures from 100 to 300 K, respectively, indicate that the electron traps in the forbidden zone of TiO₂ are shallow. The value of the donor level energy E = 0.082 eV is close to the values of the activation energy of thermal conductivity. This indicates the formation of donor centers in anodic TiO₂ by the mechanism of donor vacancies. In anodic TiO₂ films, the concentration of electron traps is 10¹⁵ cm⁻³, which is approximately three orders of magnitude less than their concentration in anodic TiO₂ films obtained by vacuum deposition. Full article

European badgers (Meles meles) represent an ideal model for studies on conflict resolution between pest control and conservation management. From the farmers’ perspective, badgers are considered agricultural pests, mainly owing to crop damages. From a veterinary and public health point of view, they are implicated in disease spread to livestock and humans. Nevertheless, at the same time, the European badger operates as an ecosystem engineer by creating new microhabitats. The scope of the present study is to evaluate the role, behavior, and public health importance of the European badger in an intensive agricultural area, in Galatas, western Greece. We used camera traps to collect ecological and behavioral data in correlation to seasonal occurrence of badgers. Furthermore, we collected badger feces samples to investigate any potential parasite load using both microscopic and molecular analyses. Lastly, we assessed farmers’ opinions regarding the occurrence of this animal on their properties, as well as any imputed damage. Interestingly, farmers’ opinion was in line with camera observations. Parasitological and molecular analyses revealed the presence of four parasites in badger feces, namely Strongyloides procyonis, Ancylostoma caninum and Perostrongylus falciformis, and a member of the family Strongyloididae. Overall, our results demonstrate the controversial role of Meles meles as an ecosystem engineer and a pest simultaneously, highlighting the value of effective monitoring preceding any control practice. Full article

Inflammation is a key feature in breast cancer progression, with neutrophil extracellular traps (NETs) playing an important role. NETs are DNA-based structures released by neutrophils that can promote tumor adhesion, invasion, and immune evasion. Another crucial mechanism is the inflammasome, a multiprotein complex that drives inflammation through cytokine release. Both mechanisms are present in tumors and may act synergistically. In this study, we evaluated how isolated NETs modulate the NLRP3 inflammasome in a human breast cancer model. Exposure of MDA-MB-231 cells to NETs increased the expression of NLRP3, CASP1, and IL1B. Blocking IL-1R with Anakinra reduced IL1B expression, while inhibition of the P2X7 receptor with A740003 decreased NLRP3 and IL1B. ELISA confirmed that NETs stimulate IL-1β release, which was reduced by MCC950, Anakinra, and A740003. Functionally, NETs accelerated tumor cell migration, and this effect was inhibited by MCC950 and Anakinra. Bioinformatics analysis of TCGA breast cancer samples showed differential inflammasome gene expression among subtypes and a positive correlation between inflammasome components and NET-related genes. These findings highlight the interplay between inflammatory and immune mechanisms in breast cancer progression and may support the development of new therapeutic strategies. Full article

The structural characteristics of the Majiatan fault–fold system in the northwestern Ordos Basin are complex, and the detailed 3D distribution of faults and their evolutionary mechanisms remain insufficiently understood, which restricts effective petroleum exploration in this region. To address this, this study utilizes high-resolution 3D seismic data comprising 20 lines (total length 753.371 km, survey grid 3 × 3 km) and drilling and logging data from 13 wells (including synthetic seismograms) to establish a detailed 3D fault model. We aim to elucidate the fault styles and the formation mechanism of the fault–fold–thrust belt. Results indicate the presence of 47 Mesozoic faults, all of which are thrust faults classified into three types. Structural traps dominate the leading transition zone, whereas lithologic–structural traps are prevalent in the Tian-huan Syncline. Laterally, from south to north, the fault occurrence transitions from west-dipping east-thrust to east-dipping west-thrust, accompanied by a shift in tectonic style from thrusting nappe to late-stage reconstruction. The stress intensity generated during the Late Cretaceous increases northward, causing deformation to shift westward. Typical fault styles observed include “y-shaped”, “flower-shaped”, and “imbricated” structures. The middle-north zones of the Majiatan area and the Hengshanbu Thrust Belt share a unified formation mechanism: initiation in the Late Triassic, main development in the Late Jurassic, initial shaping in the Late Cretaceous, and final modification in the Eocene, driven by the rotation of the Ordos Basin and shear tectonic forces. The most favorable exploration zones are identified at the junctions between the leading zone, the fault–fold zone, and weakly transformed zones. The tectonic evolution model established in this study provides a valuable reference for understanding structural complexities and guiding hydrocarbon exploration in similar fold and thrust belts globally. Full article

Retinol-binding protein 4 (RBP4), an adipokine secreted by adipose tissues, has been implicated in metabolic inflammation and insulin resistance. Type 2 diabetes (T2D) is a recognized risk factor for osteoarthritis, with both conditions characterized by chronic low-grade inflammation, suggesting potential links between metabolic disorder and joint degeneration. This study aimed to investigate whether inflammatory and metabolic stresses regulate RBP4 expression and function in joint-related cells. Murine immature chondrocyte cells (iMACs) and the mouse AT805 teratocarcinoma cell line, clone 5, that differentiates into chondrogenic cells (ATDC5), were used as in vitro models for chondrocyte cells. Rbp4 mRNA expression increased during differentiation of iMACs, with 3.6- and 2.2-fold elevations observed on days 7 and 14, respectively (p < 0.01 vs. undifferentiated controls). Inflammatory stimulation with interleukin-6 (IL-6) significantly increased Rbp4 mRNA expression in ATDC5 cells (p < 0.05 vs. vehicle), along with elevated expression of catabolic and inflammatory mediators, including monocyte chemoattractant protein-1 (Mcp1), cyclooxygenase-2 (Cox2), and matrix metalloproteinase-3 (Mmp3) (p < 0.05 vs. vehicle). Pharmacological inhibition of RBP4 using fenretinide (FEN) attenuated chondrogenic differentiation marker expression, reduced glycosaminoglycan synthesis during chondrogenic differentiation, and mitigated high-glucose-induced catabolic responses, as indicated by reduced Mcp2 (p = 0.04) and Mmp13 (p = 0.01) expression in ATDC5 cells treated with FEN compared with cells treated with the vehicle under high-glucose conditions. Furthermore, in RAW 264.7 cells, a murine macrophage cell line commonly used as an in vitro model for osteoclastogenesis, FEN significantly reduced the expression of osteoclast differentiation markers, dendritic cell-specific transmembrane protein (DC-Stamp), nuclear factor of activated T-cells, cytoplasmic 1 (Nf-atc1), cathepsin k (Cath.k), and tartrate-resistant acid phosphatase (Trap) under osteoclastogenic conditions (p < 0.01 vs. vehicle). Collectively, these findings suggest that RBP4 functions as a metabolic–inflammatory mediator influencing both cartilage and bone-remodeling processes. This study reveals a previously unrecognized role of RBP4 in regulating osteoclast-associated pathways. Targeting RBP4 may, therefore, represent a promising therapeutic strategy for delaying or preventing osteoarthritis progression, particularly in metabolically compromised conditions. Full article

Background: A new paradigm for treating glioblastoma multiforme (GBM) cells was proposed. Instead of trying to eliminate cancer cells infiltrated in the brain, this new treatment is based on attracting them into a macroporous gel-based trap, where they are retained and then irradiated with a localized, higher radiation dose. The objective of this study is to identify a cytokine combination that would attract GBM cells while considering heterogeneity among GBM cell lines. Methods: The ability of different combinations of cytokines CXCL12, IL-1β, IL-6, and EGF to stimulate the migration of the GBM cell lines U87, U87 CXCR4+, F98, and U118 was assessed with a two-layer Matrigel device that simulates the extracellular environment in brain. The accumulation of GBM cells within a cancer cell trap made from a macroporous hydrogel consisting of 1% alginate, 0.75% chitosan, and 0.05% genipin was determined. This hydrogel was grafted with RGD and features fully interconnected pores with an average diameter of 300 µm. CXCL12 is the most frequently used for attracting GBM cells. The other cytokines were chosen to enhance CXCR4 expression, the receptor for CXCL12, increase matrix metalloproteinase-2 and -9 (MMP-2 and -9) production, and promote the epithelial–mesenchymal transition (EMT), a phenotype shift that facilitates cell migration. Results: IL-1β significantly enhanced CXCR4 expression in the F98 and U118 cells. The production of MMP-2 was significantly stimulated with IL-1β and IL-6 in F98 cells. The combination of the cytokines IL-1β + IL-6 + CXCL12 + EGF, on the other hand, induced a decrease in MMP-2 levels. The EMT was induced by EGF in all GBM cells tested. The results obtained using the two-layer Matrigel device showed that the combination of the cytokines IL-1β + CXCL12 + EGF was the most effective in promoting the migration of the four GBM cell lines. Regarding accumulation in the macroporous hydrogel, U118 cells showed the best response to this cytokine combination. Conclusions: A significant challenge in developing a cancer cell trap is to identify a cytokine combination to attract the heterogeneous population of GBM cells. In this study, the cytokine combination IL-1β + CXCL12 + EGF was found to be the most effective in promoting the migration of GBM cells. Full article

Traditional involute gear pumps find it difficult to meet the requirements of miniaturization and high performance because of the undercutting, trapped oil, and flow pulsation. To eliminate the phenomenon of trapped oil and reduce flow pulsation in the miniature gear pump, a novel miniature double-circular-arc line gear (MDLG) and its manufacturing method are proposed. Firstly, based on the spatial curve meshing theory, the tooth flank equation of the MDLG is established, and the design method of the MDLG hob is presented. Then, the instantaneous flow rate of the MDLG pump is analyzed by using the swept-area method. Subsequently, a hobbing machining model is built on the VERICUT virtual simulation platform, and machining experiments are conducted on a hobbing machine. Furthermore, the manufactured MDLGs are inspected at a gear measuring center. Finally, an MDLG pump prototype is developed and machined. The measurement results show that the total cumulative pitch deviations of the machined MDLGs are controlled within 32.1 μm, achieving the ISO 8 accuracy grade. The theoretical calculations and experimental results in this article verify the feasibility of the design and processing of MDLG pumps, providing a reference for the development of high-performance miniature gear pumps. Full article

A conceptual design study is presented for a hemispherical, two-chamber water Cherenkov detector instrumented with bladder-embedded light traps. The detector consists of a rigid aluminium vessel enclosing a water volume that is divided into an outer, optically black chamber and a inner, reflective chamber lined by a flexible bladder. Arrays of light-trap modules, based on plastic scintillators with wavelength-shifting elements and thin silicon photomultipliers, are integrated into the bladder and selected inner surfaces. This geometry is intended to enhance muon tagging, increase acceptance for inclined air showers, and enable improved discrimination between electromagnetic and hadronic components. The study describes the mechanical and optical layout of the detector, the baseline aluminium housing, and the use of 3D-printed hexagonal prototypes to validate integration of the bladder and readout electronics. A first-order structural assessment based on thin-shell and plate theory is presented, indicating large safety margins for the hemispherical shells and identifying the flat base as the mechanically most loaded component. While GEANT4 simulations for detector response to extensive air showers in the atmosphere and performance measurements are left to future work, the present study establishes a mechanically validated, costed baseline design and outlines the steps needed to assess its impact in air-shower arrays. Full article

This study proposes an artificial intelligence-integrated Six Sigma framework for reducing multiple critical defects in plastic injection molding using real industrial production data from a washing-machine control-panel manufacturing line. Predictive models were developed under severe class imbalance conditions and combined with SHAP-based interpretability to identify the most influential process parameters. A multi-objective NSGA-II optimization strategy was then employed to simultaneously minimize major defect types, including gas-trapped burn (GTB), short shot (SS), sink mark (SK), and flash (FL). The proposed framework was validated through on-site continuous trial production of 300 parts after process stabilization, demonstrating substantial and consistent defect reduction. The results indicate that the integration of data-driven modeling, explainable artificial intelligence, and evolutionary multi-objective optimization provides a practical and scalable approach for quality improvement in industrial injection molding processes. Full article

Anti-GQ1b antibody syndrome (AGABS) unifies triad-defined Miller Fisher syndrome (MFS), Bickerstaff brainstem encephalitis (BBE), and the ophthalmoplegic variant of Guillain–Barré syndrome (GBS-O) under a post-infectious immune mechanism centered on IgG to disialosyl gangliosides. The spectrum also encompasses triad-minus phenotypes—acute ophthalmoparesis without ataxia, acute vestibular syndrome, optic involvement, and acute sensory-ataxic neuropathy. A molecular-mimicry model with complement-mediated nodal/paranodal dysfunction explains severe early deficits despite bland limb nerve conduction studies (NCSs), the cranial/proprioceptive predilection, and generally favorable treatment responsiveness to immunotherapy. In practice, a serology-first strategy, complemented by targeted electrophysiology—blink and H-reflex testing, and, where feasible, paired SEP–ABR showing a literature-supported dissociation (normal ABR with impaired median-nerve cortical SEPs), which, in our series, was documented in one illustrative BBE case—and by structured neuro-otologic examination, mitigates the “normal-NCS trap” and enables timely treatment. Intravenous immunoglobulin (IVIg) is first-line; plasma exchange (PLEX) is an alternative in severe or IVIg-ineligible cases; and intravenous methylprednisolone (IVMP) may be added selectively for central/optic-weighted phenotypes without routine oral taper. We consolidate actionable diagnostic and therapeutic steps and examine them in an institutional series of 16 consecutive seropositive patients (2015–2025): all were anti-GQ1b-positive with frequent GT1a co-reactivity; most reported an antecedent infection—typically upper respiratory, less often gastrointestinal—within the two weeks before onset; limb NCSs were often nondiagnostic whereas reflex/evoked-potential studies were informative; two required intubation in addition to IVIg; outcomes were generally favorable with early immunotherapy. The practical message: order anti-GQ1b at first contact, pair targeted electrophysiology with neuro-otology, and treat early to exploit reversible nodal/paranodal dysfunction. Full article

We report on the realization of a platform for trapping and manipulating individual ⁸⁸Sr atoms in optical tweezers. A first cooling stage based on a blue shielded magneto-optical trap (MOT) operating on the ${|}^1{\mathrm{S}}_0⟩\to {|}^1{\mathrm{P}}_1⟩$ transition at $461 \mathrm{n}\mathrm{m}$ enables us to trap approximately 4 × 10⁶ atoms at a temperature of 6.8 mK. Further cooling is achieved in a narrow-line red MOT using the ${|}^1{\mathrm{S}}_0⟩\to {|}^3{\mathrm{P}}_1⟩$ intercombination transition at 689 nm, bringing 5 × 10⁵ atoms down to $5\mu \mathrm{K}$ and reaching a density of 4 × 10¹⁰ cm³. Atoms are then loaded into 813 nm tweezer arrays generated by crossed acousto-optic deflectors and tightly focused onto the atoms with a high-numerical-aperture objective. Through light-assisted collision processes we achieve the collisional blockade, which leads to single-atom occupancy with a probability of about 50%. The trapped atoms are detected via fluorescence imaging with a fidelity of $99.986\left(6\right)\%$, while maintaining a survival probability of $97\left(2\right)\%$. The release-and-recapture measurement provides a temperature of $12.92\left(5\right)\mu \mathrm{K}$ for the atoms in the tweezers, and the ultra-high-vacuum environment ensures a vacuum lifetime higher than 7 min. These results demonstrate a robust alkaline-earth tweezer platform that combines efficient loading, cooling, and high-fidelity detection, providing the essential building blocks for scalable quantum simulation and quantum information processing with Sr atoms. Full article

Microalgae, such as Euglena cantabrica, are rich in secondary metabolites, including polyphenols, which are valued for their antioxidant and therapeutic properties. Here a rapid, cost-effective and efficient protocol using a trichloroacetic acid (TCA) solution was developed for the production of an extract from E. cantabrica (EuPoly). The potential environmental and biomedical applications of this new extract were evaluated. The effects of EuPoly extract were tested on normal human dermal fibroblasts (NHDFs) and on breast cancer cells of the triple-negative MDA-MB-231 cell line. EuPoly was able to increase the NHDFs survival in oxidative -stress conditions and, on the contrary, to induce a decrease in cell viability of the breast cancer cells. EuPoly was also used to functionalize frustules (FEuPoly), mesoporous silica structures from diatoms. FEuPoly were investigated for the complexation of Cu²⁺ and Ni²⁺, as new potential tools for metal-ion decontamination. Finally, the scaffolding properties of FEuPoly were here assessed in the bone marrow mesenchymal stem cells (BM-MSCs) growth and their osteo-differentiation. This study provides new insights into the sustainable valorization of algae extracts, showing that TCA E. cantabrica extract and functionalized frustules may serve as multifunctional, eco-friendly resources for biomedical applications, as antioxidants and cancer cell inhibitor, metal ions-trapping and tissue osteo-repair. Full article