Search Results (1,952)

The fabrication of complex architectures remains a central challenge in 3D bioprinting, as the low mechanical properties of hydrogels limit the range of achievable geometries. Four-dimensional (4D) bioprinting can address these limitations by enabling programmed shape-morphing behavior; however, in most approaches, this functionality is introduced after hydrogel formation, limiting the complexity of the resulting deformation. Here, a proof-of-concept strategy is presented, in which shape-morphing is directly encoded during fabrication. By modulating light exposure time layer-by-layer in vat photopolymerization, spatial variations in crosslinking density are introduced in situ within Gelatin Methacryloyl (GelMA) hydrogel constructs. Exposure times in the range of 20–70 s were investigated, enabling controlled bending of the printed structures upon immersion in aqueous media, with radii of curvature between 11 and 20 mm depending on the geometry. This approach allows deformation pathways to be programmed during printing, without requiring additional materials or post-processing steps. The morphing behavior was further supported by finite element simulations, which reproduced the experimentally observed deformation and enabled prediction of the shape change. In addition, high cell viability (>95%) was maintained after material contact and UV exposure. Overall, this study demonstrates that swelling-driven actuation can be encoded during fabrication. Although demonstrated on simplified geometries, this approach provides a versatile framework for process-driven shape-morphing and represents a step toward more spatially resolved and potentially volumetric 4D bioprinting strategies. Full article

Atlantic salmon (Salmo salar) is highly perishable during refrigerated storage due to the formation of off-odor volatile compounds that limit shelf life and consumer acceptance. This study investigated the development of off-odor volatiles in Atlantic salmon fillets during refrigerated storage and evaluated how rearing conditions influence storage-induced volatile formation. Salmon reared under warm (20.3 ± 1.95 °C with continuous light) or cool (13.1 ± 0.85 °C with a 12 h light–12 h dark cycle) conditions were harvested, stored at 4 ± 1 °C, and analyzed at 0, 3, 7, 9, and 15 days using selected-ion flow-tube mass spectrometry (SIFT-MS). Refrigerated storage was the primary driver of volatile formation, with lipid-derived aldehydes and alcohols forming early, followed by additional oxidation products as deterioration progressed, and finally, terminal oxidation products. These findings demonstrate distinct temporal pathways of off-odor volatile formation during refrigerated storage, linking early-stage oxidation of polar lipids, mid-stage involvement of neutral lipids, and late-stage accumulation of terminal and microbial products. Protein-derived volatiles exhibited compound-specific behavior, with reactive sulfur- and nitrogen-containing compounds increasing early or mid-storage and microbial metabolites accumulating steadily over time. Environmentally derived off-odor compounds, including geosmin and 2-methylisoborneol, were progressively released during storage as lipid structures degraded. Warm-reared salmon consistently exhibited higher concentrations of lipid- and protein-derived volatiles, indicating greater oxidative and proteolytic susceptibility. Rearing conditions modulate the extent but not the progression of these spoilage mechanisms. This mechanistic understanding provides a basis for targeted strategies to control off-odor volatile compound development and improve refrigerated shelf life and sensory quality of Atlantic salmon. Full article

Indoor lighting systems are a significant contributor to building energy consumption while also directly affecting occupant comfort and circadian regulation. Recent advances in smart lighting have introduced adaptive and human-centric approaches; however, the integration of optimization-oriented control strategies with interoperable automation frameworks remains only partially articulated in the literature. This study presents a systematic bibliometric review of smart indoor lighting research, with particular attention to the roles of hyper-heuristics (HH), Internet of Things (IoT) platforms, and IFTTT/Event–Condition–Action (ECA) automation. A PRISMA-based methodology was applied across Scopus, Web of Science, and IEEE Xplore for the period 2010–2025. A total of 5529 records were identified, with 5229 screened after duplicate removal, and 27 core studies included following eligibility assessment. To reduce the risk of over-interpreting null intersections, the review also incorporated a search-sensitivity analysis based on expanded query formulations and title–abstract screening. Bibliometric analysis was conducted using MATLAB and VOSviewer to identify publication trends, technological clusters, and patterns of fragmentation across the literature. The results indicate rapid growth in IoT-based and energy-aware lighting systems, alongside mature research in circadian and comfort-driven lighting. However, explicit indexed evidence connecting hyper-heuristics with IoT platforms and IFTTT/ECA frameworks remains sparse and fragmented in the available literature. Co-occurrence analysis further reveals weak metadata-level connections between optimization techniques and IoT protocols, while the sensitivity analysis confirms that broadened retrieval improves recall but still yields only limited directly relevant evidence. Overall, the review identifies a gap in the explicit convergence of optimization, interoperable IoT infrastructure, and event-driven automation for human-centric indoor lighting. On this basis, it outlines a conceptual integration framework combining hyper-heuristics, IoT middleware, and event-driven control. The findings provide a structured roadmap for future research and implementation-oriented studies aimed at improving both energy efficiency and human-centric comfort in smart indoor environments. Full article

The hole injection layer (HIL) plays a critical role in achieving high efficiency and operational stability in organic light-emitting diodes (OLEDs). As a commonly used HIL, poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) is limited by its intrinsically low electrical conductivity and mismatched work function alignment with the hole transport layer (HTL), leading to inefficient hole injection and carrier imbalance. In this work, a mild citric acid (CA) treatment is used to simultaneously enhance the conductivity of PEDOT:PSS through the partial removal of insulating PSS and tune its work function for improved energy level alignment at the anode interface. This simultaneous optimization effectively enhances the hole transport capability, successfully matching the electron transport capability to realize highly improved charge carrier balance within the device. Consequently, Ir(ppy)₃-based phosphorescent OLEDs featuring the optimally treated PEDOT:PSS HIL deliver a maximum external quantum efficiency of 20.37%, representing a 21% improvement over devices using pristine PEDOT:PSS, along with a twofold extension in operational lifetime. This strategy demonstrates a simple and controllable approach to interfacial engineering, providing practical guidance for the development of high-performance and stable OLEDs. Full article

Antimicrobial blue light (aBL) within the visible violet–blue spectrum has emerged as a promising non-chemical strategy for microbial control, yet its performance across environmentally realistic matrices and surfaces remains insufficiently characterised. Here, we evaluate a continuous-exposure aBL LED system operating within the visible 407–421 nm range for its antimicrobial efficacy against Escherichia coli K-12 MG1655 and Bacillus cereus NCTC 11143 across liquid cultures, agar surfaces, and representative built-environment materials (glass and steel bar). Bacterial inactivation was quantified using culture-based enumeration and flow cytometric viability profiling. The system delivered a controlled irradiance of 0.72 mW/cm² at 58 cm, corresponding to cumulative doses of 2.59–62.23 J cm⁻² over 1–24 h of exposure. Significant, time-dependent reductions in viability were observed across all matrices relative to fluorescent-light controls, with near-complete or complete loss of recoverable cells on solid surfaces following prolonged exposure. Flow cytometric analyses revealed progressive transitions from viable to injured and dead cell populations, consistent with photodynamic inactivation mediated by endogenous photosensitiser activation and reactive oxygen species generation. These findings demonstrate that continuous visible-light aBL illumination can achieve effective multisurface microbial inactivation under moderate irradiance conditions compatible with occupied environments, supporting its translational potential as a sustainable, non-chemical decontamination strategy for healthcare, food-processing, and built environments. Full article

This study evaluated the effects of light spectral quality on shoot yield and essential oil of Tagetes erecta L. cultivated in controlled growth chambers under three LED lighting treatments with different red, blue, and white wavelength ratios and a constant 16 h photoperiod for up to 101 days. The F2 treatment (5 red:1 blue) produced yields of fresh shoots, early blooming flowers, and oils of 1586 ± 164 g/m², 569.77 ± 76.81 g/m², and 307 ± 31.7 mg/m², respectively. These values were significantly higher (p < 0.05) than those of the F1 treatment (white:red-phosphor), and represented increases of 1.37-, 1.26-, and 1.38-fold, respectively. Gas chromatography identified 30–31 compounds in the oil with three major constituents—(E)-β-ocimene (22.9–28.8%, highest under F3), (E)-myroxide (13.9–20.6%, highest under F1), and piperitone (7.3–9.6%, highest under F3). Essential oils inhibited from four to five of the seven tested microbial strains, with the notable activity against Escherichia coli and Candida albicans recorded in F2 and F1, respectively. These findings confirm that light spectral quality is a critical factor regulating flower, essential oil, and antimicrobial efficacy in T. erecta, demonstrating that optimized LED spectra offer a practical strategy to improve plant yield and phytochemical quality. Full article

Chlorogenic acid (CGA) is a natural polyphenol with various biological activities, but its poor stability and premature release in the gastrointestinal tract limit oral application. Herein, a pH-responsive bilayer hydrogel based on sodium alginate (SA) and carboxymethyl cellulose (CMC) was developed to enhance the gastrointestinal stability and controlled release of CGA. CGA-loaded SA hydrogels were prepared via Ca²⁺-induced ionotropic gelation, followed by CMC coating to form a bilayer structure. The SA/CMC hydrogels showed a drug loading capacity of 15.2–16.7% and pH-dependent swelling behavior. In vitro release studies revealed that the bilayer hydrogel suppressed CGA release in simulated gastric fluid (pH 1.2), with a cumulative release of approximately 30%, while enabling sustained release in simulated intestinal fluid (pH 6.8), reaching about 70% within 10 h. Release kinetics indicated that CGA release was controlled by Fickian diffusion under acidic conditions and by a diffusion-polymer relaxation mechanism under intestinal conditions. Moreover, encapsulation in the SA/CMC hydrogel improved the thermal, light, and pH stability of CGA while maintaining its antioxidant activity and biocompatibility. These results indicate that SA/CMC bilayer hydrogels provide a promising strategy for stabilized gastrointestinal delivery of chlorogenic acid. Full article

The enrichment of chocolate with healthy beneficial ingredients represents an effective strategy to create functional food with high nutritional and bioactive potential. Comparisons were made between eight treatments derived by the factorial combination of 2 types of butter (milk and cocoa) and 4 concentrations of green barley powder addition (1%, 3%; 5%; and 7%), plus 2 untreated controls (milk butter and cocoa butter with no green barley powder addition), in terms of chemical, colorimetric, physical, antioxidant, mineral and sensory characteristics of white chocolate. Increasing addition of green barley to both milk and cocoa butter led to the decrease in dry matter, soluble solids, pH and fat in the produced chocolate, with the untreated controls always showing the highest values. Opposite trends were recorded for proteins, fiber, ash and mineral substances. The ‘L’, ‘a’ and ‘b’ color components gradually decreased from the untreated control to the highest concentration of barley powder addition both to milk and cocoa butter. The increasing integration of barley powder either into milk or cocoa butter resulted in the gradual decrease in F max compression and F max cutting of the chocolate manufactured, compared to the untreated control. The addition of barley powder to milk and cocoa butter elicited a gradual increase in all the antioxidants analyzed, i.e., vitamin C, carotenes, lycopene and xanthophylls, and of chlorophyll a and b, compared to the untreated control. Vegetal flavor attributes were enhanced by the increasing addition of green barley powder. The latter incorporation into milk and cocoa butter sheds light on the interesting topic of conceiving and applying the manufacture of innovative functional chocolate with high content of fiber, nutrients and antioxidants. Full article

Molecular on-surface photochemistry has emerged as a promising alternative to thermal activation for fabricating low-dimensional carbon-based nanomaterials, offering unique advantages such as non-thermal initiation and high chemoselectivity. Controlling the selectivity and efficiency of on-surface photoreactions remains challenging due to the complex interplay among molecular excitation pathways, substrate properties, and reaction conditions. This review briefly summarizes recent advances in light-driven on-surface synthesis under ultra-high-vacuum conditions. We focus on molecular photoexcitation pathways that can be probed by scanning tunneling microscopy and spectroscopy (STM and STS). Studies of light-driven reactions in three categories are overviewed, i.e., dehalogenative C-C coupling, [2+2] and [4+4] cycloadditions, and photoisomerization. Typical strategies for tuning reactivity are exemplified, including molecular pre-organization via self-assembly, surface passivation, and wavelength/polarization control. The summary of successful case studies may not only facilitate the fundamental understanding of on-surface photochemistry but also inspire the design of functional low-dimensional architectures and light-responsive molecular devices. Full article

Artificial lighting accounts for roughly 30% of total electricity use in supermarkets and significantly affects product perception, customer experience, and purchasing behavior. Increasing the availability of natural light, combined with appropriate architectural energy retrofitting strategies, offers a major opportunity to reduce electricity demand. This study proposes a data-driven framework for evaluating energy retrofit strategies in commercial buildings, integrating Building Information Modeling (BIM) and Building Energy Modeling (BEM). A parametric methodology is used to evaluate multiple architectural retrofitting scenarios aimed at enhancing daylighting and reducing artificial lighting demand, while improving energy efficiency and environmental performance. The scenarios investigated include variations in skylight geometry and orientation, glazing type, photovoltaic integration, and advanced lighting controls. Three Key Performance Indicators (KPIs)—real energy effectiveness, lighting control performance, and environmental impact—are used to assess how design modifications influence energy use, indoor lighting quality, and environmental performance. The methodology is applied to three real food-retail buildings in Italy. Results show that lighting energy consumption can be reduced by up to 60% in scenarios combining LED technology with smart control systems, while total building electricity savings vary across case studies depending on building characteristics and usage patterns. Environmental impact reductions of approximately 15–20% are achieved, reflecting both operational and life-cycle improvements. The study demonstrates the potential of parametric architectural retrofitting to support multi-criteria decision-making for sustainable refurbishment of food-retail environments. Full article

Autonomous driving in underground mines faces significant challenges due to Global Navigation Satellite System (GNSS) denial and harsh environmental conditions. Mainstream multi-sensor fusion and Simultaneous Localization and Mapping (SLAM) schemes have achieved substantial progress in underground navigation, but their deployment in feature-sparse tunnels may still face challenges related to computational burden and perception robustness. This study explores an infrastructure-assisted navigation architecture that transforms the roadway into a structured luminous guidance channel by deploying programmable Light Emitting Diode (LED) strips along the tunnel roof. The proposed system simplifies complex three-dimensional pose estimation into a two-dimensional visual servoing task targeting optical signals. Central to this approach is a robust data fusion strategy that utilizes a topology matching algorithm to map noisy Ultra-Wide-band (UWB) coordinates onto a discrete LED index space, thereby providing a reliable global positioning reference. Furthermore, a hierarchical fault-tolerant controller based on a Finite State Machine (FSM) is designed to facilitate seamless degradation to a UWB-assisted ultrasonic wall-following mode in the event of visual degradation, supporting fault-tolerant operation under controlled laboratory conditions. Experimental results in a laboratory simulation environment demonstrate that the system achieves millimeter-level static initialization accuracy, a dynamic tracking Root Mean Square Error of approximately 4 cm, and a 100% autonomous recovery rate from visual failures in straight tunnels. These results demonstrate the feasibility of the proposed infrastructure-assisted route under controlled laboratory conditions and suggest its potential as an engineering reference for structured underground transport scenarios with acceptable infrastructure modification. Full article

Plant extracts are widely used as natural pesticides, cosmetic ingredients, and in pharmaceutical applications. However, their poor water solubility and stability limit their usability. Lipid nanoparticles (LNPs) offer an effective encapsulation strategy to overcome these challenges. This study demonstrates the encapsulation of three representative substances from these industries: quercetin as a pesticide, irones as a cosmetic ingredient, and nucleic acids for pharmaceutical use. Ultrasonic treatment was used for the encapsulation of quercetin and irones, and a concept for continuous encapsulation in a plug flow reactor was proposed for process intensification. Inline multi-angle light scattering and dynamic light scattering measurements proved effective for real-time monitoring and enabled the replacement of traditional batch measurements. In the pharmaceutical area, mRNA-based therapies require LNP encapsulation to prevent nucleic acid degradation. Plant-based β-sitosterol was used as an alternative helper lipid to cholesterol, resulting in an average particle diameter of 72 nm and an encapsulation efficiency of 91%, comparable to commercial formulations such as the Comirnaty vaccine. Furthermore, a novel process model based on population balances was developed to simulate the entire manufacturing process, from rapid mixing in a T-mixer to particle stabilization via buffer exchange during diafiltration. By applying a quantitative and distinctive model validation workflow, the model was shown to be as accurate and precise as the experimental data, enabling its use as a digital twin for autonomous continuous operation. In summary, this study contributes to reducing the facility footprint and cost of goods through the implementation of continuous processing and model-based control. This approach improves productivity by 20% and reduces process time by a factor of two. Full article

Fruit anthocyanins are primary determinants of color, sensory quality, and nutritional value in grapes; however, their endogenous biosynthesis is governed by complex interactions among genetic, environmental, agronomic, and postharvest factors. This review elaborates recent advances in physiology and molecular biology to clarify the biosynthetic mechanisms in grapes, including the coordinated action of structural enzymes, MYB–bHLH–WD40 regulatory complexes, hormone-mediated signaling pathways, and vacuolar transport processes. Key environmental factors, such as temperature fluctuations, light exposure, water availability, and soil properties, regulate these networks, contributing to significant variation in pigmentation profiles across cultivars and growing regions. Strategic agronomic practices, including canopy management, regulated deficit irrigation, balanced nutrient management, and temperature-mitigation techniques, further influence pigmentation by modifying the microclimate of the fruit zone during development. Based on these mechanistic insights, this review evaluates targeted strategies for enhancing anthocyanin accumulation, highlighting recent progress in genetic improvement through CRISPR/Cas genome editing, transgenic approaches, and marker-assisted selection (MAS), which enable precise modulation of biosynthetic and regulatory genes. Complementary postharvest interventions, such as optimized cold storage, modified-atmosphere packaging, hormonal elicitors, and controlled oxidative technologies, provide additional opportunities to maintain or enhance pigment stability after harvest. Collectively, these advances establish a comprehensive framework linking molecular regulation with practical vineyard, breeding, and postharvest strategies, offering an integrated pathway to improve anthocyanin consistency, berry quality, and the phenolic characteristics of grape-derived products. Full article

This paper addresses the issue of lighting in historic urban spaces, using Tarnow (Poland) as a case study. The aim is to assess the impact of artificial light sources on visual comfort within the area, with particular consideration given to light pollution. A comprehensive inventory of active street lighting in the Old Town was conducted. Measurements taken at ground and eye level revealed strong inconsistencies: some areas were under-lit (<1 lx), while others showed façade illuminance above 100 lx, far exceeding recommended thresholds. The highest environmental impact was shown by decorative and globe-type fixtures, with Sky Glow Contribution Index (SGCI) values of up to 0.62. Only suspended street luminaires met CIE requirements (ULR ≤ 15%). The findings reveal that several lighting installations do not meet recommended standards, adversely affecting both human comfort and ecological balance. The study proposes strategies to optimise urban lighting, such as replacing inefficient fixtures with full cut-off LED luminaires and implementing intelligent lighting control systems which could reduce energy consumption by 50-67% while preserving the architectural character of the historic centre. The results provide evidence-based strategies for sustainable lighting modernisation in heritage cities across Europe. Full article

Background: We synthesized evidence from randomized clinical trials (RCTs) published between 2019 and 2025 on repetitive transcranial magnetic stimulation (rTMS) in post-stroke cognitive impairment (PSCI) and compared different stimulation parameters, cortical targets, and combinations with rehabilitation interventions. Methods: A systematic review according to PRISMA guidelines examined the RCTs applying rTMS in adults with PSCI compared with control or sham groups. The primary outcome was improvement in cognitive function and functional outcomes measured with standardized scales. Results: Fifteen studies, involving a total of 732 patients, were included. The most frequently investigated were high-frequency (≥10 Hz) stimulation protocols of the left dorsolateral prefrontal cortex, with treatment cycles ranging from 2 to 6 weeks. Overall, rTMS was generally safe and well tolerated, with rare and mild adverse events. Several studies reported improvements in cognitive performance following rTMS, although effects were variable across trials and need caution in light of heterogeneity in stimulation protocols, sample sizes, outcome measures, and methodological quality. In most cases, rTMS or intermittent theta burst stimulation combined with structured cognitive training yielded greater cognitive and functional gains than stimulation or rehabilitation alone. This suggests a positive interaction between rTMS and cognitive training, although current evidence does not yet allow definitive conclusions. Conclusions: rTMS appears to be a promising strategy for post-stroke cognitive rehabilitation, particularly for attention and executive functioning. However, heterogeneity in stimulation protocols and outcome measures, along with limited sample sizes and short follow-up, reduces the certainty and comparability of current evidence. The widespread reliance on global screening tools may further underestimate domain-specific effects. Future multicentre trials with standardized protocols and more sensitive cognitive assessments are needed to clarify efficacy and guide further clinical application of rTMS in PSCI. Full article