Search Results (238)

The increasing consumption of fresh organic produce has given rise to concerns regarding the microbiological safety of minimally processed foods. Organic cultivation may be associated with increased exposure to environmental microorganisms due to soil-based inputs and reduced chemical interventions, including both beneficial taxa and potential foodborne pathogens. Fresh produce is known to harbour complex microbial ecosystems, which are shaped by farming practices, plant physiology, handling, packaging and storage, particularly in raw-consumed products such as leafy greens and strawberries. In this study, bacterial (16S rRNA) and eukaryotic (18S rRNA) communities were characterized by amplicon sequencing. In parallel, an amoeba-associated bacterial microbiome was analyzed and DVC-FISH was used to assess the viability and metabolic activity of pathogenic bacteria internalized within free-living amoebae (FLA). No significant differences in alpha or beta diversity were observed between organic and conventional products, suggesting microbiome convergence at the retail stage driven by post-harvest handling and processing. Potentially pathogenic genera, including Pseudomonas, Stenotrophomonas, and Acinetobacter (bacterial), as well as Tilletiopsis, Candida, and Naegleria (eukaryotic), were identified in both organic and non-organic microbiomes. The viability of FLA-internalized Pseudomonas spp. was confirmed by DVC-FISH, demonstrating that FLA act as reservoirs, enhancing pathogen persistence in fresh produce. This integrated assessment of organic and conventional fruits and vegetables at the retail stage highlights the importance of post-harvest handling and retail conditions in shaping microbiological safety. The integration of microbiome profiling with targeted viability analyses demonstrates that downstream stages are critical control points for food safety and consumer exposure, beyond the influence of the production system alone. Full article

Cultivation knowledge deficiencies limit the appreciation of microalgae-based nutrient solutions on hydroponic plants. This study compared Chlorella vulgaris implications for lettuce growth and the production of high-value components through the use of four different co-cultivation hydroponic scenarios. The results of 30-day co-cultivation of Chlorella vulgaris and lettuce demonstrated the significance of controls of pH (7.0–7.75) and green microalgal cell density (10⁷ cells/mL) to improve the qualities in lettuce leaf growth, root vigor, and nutrient yield from days 15 to 30 during the co-cultivation. Plant height increased by 19%, leaf area by 4%, root cortex thickness by 14% (p < 0.05), total chlorophyll content by 49%, soluble sugar content by 12%, and protein content by 6% through the adoption of 1.0 × 10⁷~1.6 × 10⁷ cells/mL of microalgal solution during hydroponic cultivation. Furthermore, the aerated hydroponic device benefits of co-cultivating high-concentration Chlorella vulgaris and lettuce resulted in a 1.0-time increase in vitamin C compared to the cultivation of low-concentration Chlorella vulgaris. This study highlights the benefits of the sustainable strategy of the microalgal cultivation technique used in the hydroponic systems for nutritious and healthy leafy vegetable growers, which is also emphasized for eco-friendly bioactive compound production. Full article

Background/Objectives: Cognitive function is fundamental to daily life, and nutrition is a key modifiable determinant of brain health across the lifespan. While plant-based “brain foods” have been emphasized, the contributions of animal-sourced foods (ASF) to neurodevelopment and cognitive performance remain underexplored. This review synthesizes current evidence on the effects of both plant- and animal-derived foods on cognitive outcomes from early development through older adulthood. Methods: A narrative review was conducted focusing on eight major categories of brain-supportive foods—dairy, eggs, seafood, lean meat, berries, leafy green vegetables, nuts, and whole grains. Evidence was evaluated across life stages, considering nutrient bioavailability, dietary patterns, and the interplay between structural, socioeconomic, and environmental factors that influence access to these foods. Results: Nutrient-dense foods, including ASF and plant-based sources, support cognitive outcomes across the life course. In early childhood, eggs, meat, and nuts were linked to improved neurodevelopment and reductions in developmental delays, while evidence for seafood and dairy was more mixed. During adolescence and adulthood, berries, walnuts, vegetables, and whole grains were associated with improvements in executive function, verbal reasoning, and mood, with adequate bioavailable protein from ASF remaining important. Among older adults, higher intake of leafy greens, nuts, berries, and moderate seafood consumption correlated with slower cognitive decline and improved memory. Findings were limited by heterogeneous study designs, dietary assessments, and underrepresentation of adolescents and populations in low- and middle-income countries. Conclusions: Both animal-sourced and plant-based brain foods uniquely support cognitive development, maintenance, and resilience. While nutritional needs vary across the life course, strong evidence supporting distinct food-based dietary recommendations for cognitive outcomes at different ages, particularly adolescents, remains limited. Current findings suggest stage-specific associations, particularly during early development, but more longitudinal and experimental research is needed. Expanding rigorous, inclusive research will be critical for informing nutrition policies that support lifelong cognitive health. Full article

In controlled-environment agriculture (CEA), light serves both as an energy source for photosynthesis and as a regulatory factor. However, the light responses of underutilized leafy greens are still not fully characterized compared with model crops such as lettuce. This study evaluated the effects of lighting parameters on the growth, metabolism, antioxidant properties, and mineral composition of Chrysanthemum coronarium (shungiku) greens cultivated hydroponically in CEA. Three parallel experiments were conducted, aiming to explore the effects of (I) light spectrum using red (R, 660 nm), blue (B, 447 nm), and combined RB light; (II) photoperiod, using 12, 16, and 24 h photoperiods at equal daily light integral; and 150, 200, 250, and 300 µmol m⁻² s⁻¹ photosynthetic photon flux density (PPFD) at 16 h photoperiod. RB light promoted the highest biomass accumulation and light use efficiency (LUE), while monochromatic red and blue light limited growth and reduced Fe and Zn contents. A 12 h photoperiod yielded the best results for leaf area, fresh weight, and LUE compared with 16 and 24 h photoperiods. Higher PPFD increased biomass, soluble sugars, antioxidant capacity, organic acids, and micronutrients, with peak LUE at 200 µmol m⁻² s⁻¹ instead of the maximum yield at 300 µmol m⁻² s⁻¹. These findings emphasize the importance of crop-specific and trait-oriented light optimization for underutilized leafy vegetables. Full article

The Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet has emerged as a promising dietary pattern associated with reduced Alzheimer’s disease (AD) risk, supported by growing evidence that both diet and the gut microbiota are modifiable contributors to disease development and progression. Observational studies have linked higher MIND diet adherence to lower AD incidence and slower cognitive decline, with certain comparative analyses reporting stronger associations with cognitive outcomes than those observed for the parent Mediterranean or DASH diets. Developed specifically to support cognitive health, the MIND diet emphasizes leafy green vegetables, berries, and olive oil while restricting butter, cheese, fried foods, sweets, and red meat. While these features suggest a biologically plausible basis for neuroprotection, the underlying mechanisms remain incompletely defined. The microbiota–gut–brain axis offers a potential mechanistic framework, as diet is a major determinant of gut microbiota composition and microbiota-derived metabolites that may influence brain function and AD-related pathways. However, direct evidence characterizing MIND diet-specific effects on the gut microbiota remains limited, with most mechanistic insights derived from related dietary patterns or individual dietary components. Accordingly, this review synthesizes evidence from these related dietary patterns and key MIND components to propose a conceptual framework linking the MIND diet, the gut microbiota, and AD risk, while highlighting priorities for future research. Full article

Optimizing nutrient management in organic polytunnel production remains challenging due to the limited availability of field-based knowledge on the mineralization dynamics of organic fertilizers. At the same time, microalgae-based products such as Chlorella vulgaris have gained increasing attention in recent research, yet their interactions with nutrient supply intensity are not well understood. This study aimed to evaluate the effects of increasing nutrient supply intensities (34, 116, and 189 kg ha⁻¹ N from different organic sources), in combination with C. vulgaris foliar application, on the crop performance of kapia pepper and a subsequent leafy green crop under on-farm organic polytunnel conditions on soil with moderate organic matter content. Increasing production intensity did not result in significant improvements in pepper yield or vegetative biomass (p > 0.05), and no significant residual effects of nutrient supply were detected in the yield of the subsequent leafy green crop (p: 0.08–0.94). C. vulgaris treatment showed predominantly non-significant but positive trends in several parameters, but only in combination with high-intensity technology, while reducing the total pest damage of the thrips and stinkbug index up to 15.7% in most technology variations. These results indicate that the effects of C. vulgaris may be strongly context-dependent and confirm that increasing the intensity of nutrient supply may carry the risks of conventionalization of organic farming practices. Full article

Background: Many countries in sub-Saharan Africa are at risk of inadequate calcium intake, yet no data exist for vulnerable population groups in Madagascar. We aimed to assess daily calcium intake, the major contributing food sources, and the prevalence of inadequate intake in young children, adolescents, and women of reproductive age. Methods: The 2024 National Micronutrient Survey used a two-stage probabilistic design across all 23 regions. The daily calcium intake was estimated using a food frequency questionnaire that focused on calcium-rich foods that are commonly consumed in Madagascar and the calcium concentration measured in drinking water. Results: Calcium intake was low across all population groups, averaging 200–300 mg/d in adolescents and women and below 180 mg/d in young children. The prevalence of inadequate intake exceeded 96% in every population group. While calcium intake increased with increasing household wealth in children, the opposite pattern was observed for adolescents and women, whose intake decreased with increasing wealth. The main contributors to calcium intake were cassava leaves, cassava roots, small fresh and dried fish eaten with bones, drinking water across all population groups, and breastmilk in young children. Conclusions: The calcium intake is low throughout Madagascar and across all demographic groups. Strategies to improve intake are urgently needed and should include promoting continued breastfeeding and the consumption of calcium-rich, locally available, affordable foods such as small fish eaten with bones and leafy green vegetables, alongside a consideration of wheat flour fortified with calcium. Full article

Evidence on modifiable post-diagnosis factors influencing outcomes after intravesical Bacillus Calmette–Guérin (BCG) therapy for high-risk non-muscle-invasive bladder cancer (NMIBC) is limited. In this exploratory, feasibility-focused prospective multicenter cohort (March 2023–November 2024), BCG-naïve patients completed repeated interviewer-administered 24 h dietary recalls; prespecified food groups, selected foods, and nutrients were screened for associations with 1-year intravesical recurrence using Firth’s penalized logistic regression adjusted a priori for age, sex, and total energy intake, with false discovery rate control within each exposure family. Forty-six patients were enrolled; 41 had evaluable recurrence status, including 8 recurrences (19.5%). Participants were predominantly overweight (mean body mass index (BMI) 28.4 kg/m²) and had low adherence to a Mediterranean dietary pattern (median Mediterranean Adequacy Index 2.25). No dietary exposure met the within-family false discovery rate threshold; the smallest q-value was 0.361. Nominal inverse associations were observed for leafy green vegetables (OR per 1 SD 0.385; 95% CI 0.101–0.972) and for energy-adjusted zinc (OR 0.280; 95% CI 0.069–0.802) and magnesium intakes (OR 0.260; 95% CI 0.045–0.872), but these did not remain significant after FDR adjustment. These exploratory signals warrant replication in larger, biomarker-informed cohorts incorporating dietary biomarkers and immune profiling during BCG. Given the limited sample size and low number of recurrence events, these findings are strictly hypothesis-generating and should not be interpreted as evidence of definitive protective or risk dietary factors. Full article

Leafy green vegetables provide important nutrients for human growth; however, human health is highly compromised through consumption of vegetables contaminated by heavy metals. Therefore, the study aimed to investigate the bioaccumulation of heavy metals in five different leafy green vegetables and soils and determine the human health risks that may arise from consuming those vegetables from Tonga town in Mpumalanga province, South Africa. Soils and five edible leafy vegetables (i.e., lettuce, cabbage, rape, pumpkin leaves, and spinach) were assessed for bio-concentration factor, daily intake of metals, health risk, and target hazard quotient across the study sites. The Si, K, Na, Ca, Mg, Al, and Fe concentrations were high in the soils. In general, vegetables exhibited elevated Ca, Fe, Si, Al, and Sr levels, although spinach had high Na concentrations. The bioconcentration factor showed the following trends: Mg > B > Si > V for trace metals and Cr > Co > Mn > Ni > B for heavy metals in lettuce, spinach, and pumpkin leaves. The human risk index for all vegetables showed that all metals were not likely to induce any health hazards to humans, and the target hazard quotient for B, Si, V, Al, Cr, Mn, Fe, Ni, Zn, and Pb showed potential for substantial health risk hazard. The findings of this study generally reveal that the concentrations of the analysed metals exceeded the permissible limits established by the World Health Organisation and the Food and Agricultural Organisation. Given the high levels of metals detected in the soil and vegetables within the study area, it is important to investigate the potential implications for human health and mitigate both acute and chronic health challenges associated with heavy metal exposure. Furthermore, this study will guide policymakers in developing improved regulations and safety standards for agricultural practices and environmental protection, particularly for vulnerable peri-urban and rural communities. Full article

Accurate measurement of plant height in leafy vegetables is challenging due to their short stature, high planting density, and severe canopy occlusion during later growth stages. These factors often limit the reliability of single-plant monitoring across the full growth cycle in open-field environments. To address this, we propose a multi-temporal point cloud alignment method for accurate plant height measurement, focusing on Choy Sum (Brassica rapa var. parachinensis). The method estimates plant height by calculating the vertical distance between the canopy and the ground. Multi-temporal point cloud maps are reconstructed using an enhanced Oriented FAST and Rotated BRIEF–Simultaneous Localization and Mapping (ORB-SLAM3) algorithm. A fixed checkerboard calibration board, leveled using a spirit level, ensures proper vertical alignment of the Z-axis and unifies coordinate systems across growth stages. Ground and plant points are separated using the Excess Green (ExG) index. During early growth stages, when the soil is minimally occluded, ground point clouds are extracted and used to construct a high-precision reference ground model through Cloth Simulation Filtering (CSF) and Kriging interpolation, compensating for canopy occlusion and noise. In later growth stages, plant point cloud data are spatially aligned with this reconstructed ground surface. Individual plants are identified using an improved Euclidean clustering algorithm, and consistent measurement regions are defined. Within each region, a ground plane is fitted using the Random Sample Consensus (RANSAC) algorithm to ensure alignment with the X–Y plane. Plant height is then determined by the elevation difference between the canopy and the interpolated ground surface. Experimental results show mean absolute errors (MAEs) of 7.19 mm and 18.45 mm for early and late growth stages, respectively, with coefficients of determination (R²) exceeding 0.85. These findings demonstrate that the proposed method provides reliable and continuous plant height monitoring across the full growth cycle, offering a robust solution for high-throughput phenotyping of leafy vegetables in field environments. Full article

Smallholder farmers in semi-arid regions worldwide face persistent water scarcity, declining soil fertility, and increasing climate variability, which constrain food production. This study investigated soil and water management practices and their effects on soil health, crop productivity, and adoption among smallholder vegetable farmers in a semi-arid area in Kenya. A mixed-methods approach was employed, combining survey data from 397 farmers with a randomized field experiment. Results showed that hand watering (88.7%) and manure application (95.5%) were prevalent, while only 5.7% of farmers used drip irrigation. Compost and mulch treatments significantly improved soil organic carbon (p = 0.03), available water capacity (p = 0.01), and gravimetric moisture content (p = 0.02), with soil moisture conservation practices strongly correlated with higher yields in leafy green vegetables (R = 0.62). Despite these benefits, adoption was hindered by high water costs (42.6%) and unreliable sources (25.7%). Encouragingly, 96.2% of respondents expressed willingness to pay for improved water systems if affordable and dependable. The findings stress the need for integrated water–soil strategies supported by inclusive policy, infrastructure investment, and gender-responsive training to enhance resilience and productivity in smallholder farming under water-scarce conditions across sub-Saharan Africa and other regions globally, contributing to global sustainability targets such as SDG 6, 12 and 15. Full article

Cabbage (Brassica oleracea L. var. capitata) is an important leafy vegetable crop, and the development of homozygous parental lines is essential for F₁ hybrid breeding. Isolated microspore culture (IMC) provides a rapid approach for producing haploid and doubled haploid (DH) lines. However, its efficiency in cabbage remains highly dependent on genotype, donor plant growth conditions, and culture conditions. This study aimed to optimize key factors affecting microspore embryogenesis and plant regeneration in a Korean green cabbage (‘SJ-Ca 13’) and to evaluate the ploidy and genetic characteristics of regenerated plants. Microspore yield and embryogenesis were strongly influenced by flower bud size. Bud size of 4.0 ± 0.5 mm yielded the highest number of microspores (4.17 × 10⁴ per bud) and exclusively produced microspore-derived embryos (2.33 embryos per Petri dish), whereas smaller or larger buds failed to induce embryogenesis. Heat shock treatment at 32.5 °C was essential for embryogenesis, with 24 or 48 h of treatment inducing embryo formation, while prolonged exposure (72 h) completely inhibited embryogenesis. Efficient shoot regeneration was achieved when microspore-derived embryos were cultured on semi-solid MS medium with reduced salt strength (1/3×) and higher agar concentration (1.0%), resulting in the highest shoot regeneration rate. Ploidy test revealed that 50% of regenerated plants were spontaneous doubled haploids. SSR analysis using 26 markers detected no genetic polymorphism among regenerated plants. Overall, this study establishes an efficient IMC and regeneration system for cabbage and demonstrates its potential for rapid DH line production to support cabbage breeding programs. Full article

Background/Objectives: Infertility is a multifactorial condition with an etiopathology that remains largely unclear. Although substantial evidence implicates oxidative stress (OS) as a key contributor to both male and female infertility, targeted strategies for OS-mediated reproductive dysfunction are still not well defined and require further investigation. Ubiquinol is the reduced form of Coenzyme Q10 involved in mitochondrial bioenergetics. It can be synthesized by humans endogenously or provided by dietary sources—typically egg yolks, oily fish, organ meats, and in smaller amounts in nuts and seeds and leafy green vegetables. The present article reviews possible mechanisms through which Ubiquinol plays a role in the regulation of fertility and reproduction, discussing why it could be positioned as a conditionally essential nutrient. Several questions and areas for further inquiry are also proposed. Methods: The present position paper narratively summarizes evidence related to Ubiquinol fertility and reproduction, focusing on the literature from PubMed, Science Direct, and Semantic Scholar. Results: Research advancements suggest that when physiological demands rise during certain life stages, e.g., the reproductive years, the amount of Ubiquinol produced internally may not be enough to meet heightened needs, particularly with advanced maternal/paternal age. This places a heavier reliance on obtaining Ubiquinol from the diet, thus presenting itself as a conditionally essential nutrient during certain life stages. Conclusions: Overall, Ubiquinol appears to enhance mitochondrial energy production and antioxidant defense in gametes, a process that appears to aid sperm function, oocyte quality, and early embryo development. Collectively, these data indicate a key physiological role for Ubiquinol in male and female fertility, especially given its age-related decline. Full article

This study evaluated the dissipation kinetics and dietary risk of etofenprox in kale (Brassica oleracea) and red mustard greens (Brassica juncea), leafy vegetables frequently reported to exceed residue limits in Korea. Field trials were conducted at three sites, and residues were analyzed using QuEChERS extraction followed by LC–MS/MS in accordance with MFDS and SANTE guidelines. The method validation parameters—specificity, linearity, limit of quantitation, accuracy, and precision—were within the acceptable criteria specified by the guidelines. The half-lives of etofenprox under greenhouse conditions were 2.2 days in kale and 3.1 days in red mustard greens, with dissipation rate constants of 0.3118 and 0.2232, respectively. Dietary risk assessment based on residue levels and consumption data confirmed that the %ADI values at the pre-harvest interval (PHI, 7 days) for were <1% the average consumer group and <4% for the high-intake group. Accordingly, the residue levels were considered safe, indicating that compliance with recommended application practices poses negligible health risk to consumers. Full article

Prebiotics, such as short- and long-chain fructans, beneficially modulate the microbiota; however, individual variability in response remains unclear. In this randomized, double-blind, placebo-controlled trial, 40 healthy adults received either a combined fructan supplement—1-Kestose (Kes) and inulin (Inu)—or a placebo (maltose + cornstarch) for 4 weeks. We investigated the fecal microbiome, bacterial growth, and glycoside hydrolase family 32 (GH32) gene abundance, and further examined the association between dietary intake and GH32. Kes and Inu co-supplementation selectively increased Bifidobacterium adolescentis and B. longum, harboring the GH32 genes inuA and cscA, respectively. Growth assays revealed that B. longum, which expresses cscA, grew only on Kes, whereas B. adolescentis, which expresses inuA, showed growth on Kes and Inu. Only responders—participants showing increases in both species—exhibited consistent upregulation of GH32 genes and were associated with higher retinol and C16:3 (n-6) fatty acid intake, as well as greater green leafy vegetable and canned tuna consumption. This study provides insights into species level responses to prebiotics, supporting personalized dietary strategies for gut microbiota modulation. Full article