Search Results (250)

One-dimensional fibrous scaffolds with tunable bioactivity offer promise for bone tissue regeneration, yet optimal calcium phosphate phases for enhancing osteogenic performance remain underexplored. This study aimed to evaluate the impact of monetite-, brushite-, and cerium-doped phosphate deposition on electrospun nylon nanofibres functionalised via matrix-assisted pulsed laser evaporation (MAPLE). Five nylon fibre compositions were synthesised, coated with three calcium phosphate phases, and calcined at varying temperatures (500–800 °C) before laser deposition. Physicochemical properties were assessed using energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), and fibre diameter measurements, averaging $62.1\pm 23.8$ nm. Biocompatibility assays following MC3T3 preosteoblast seeding and incubation evaluated biological performance. EDX confirmed homogeneous phase deposition; SEM showed phase- and temperature-dependent morphology, with monetite yielding uniform granular structures and cerium-doped phosphate at 800 °C forming dense aggregates. Brushite-coated fibres exhibited superior preosteoblast metabolic activity, reaching $178\pm 2\%$ after 48 h (p < 0.001), indicating phase-specific stimulation of bone cell growth. These phosphate-functionalised nylon fibres retain structural integrity, hierarchical porosity, and enhanced bioactivity, providing a versatile electrospinning-MAPLE platform for customisable bone grafts with clinical potential. Full article

Inadequate surface sanitization represents a significant risk to sterility assurance and regulatory compliance. Therefore, an effective cleaning and disinfection program is a critical component of contamination control strategies in pharmaceutical facilities manufacturing sterile medicinal products. This study aimed to standardize a carrier-based methodology for evaluating the efficacy of disinfectants against in-house environmental isolates recovered from a pharmaceutical industry facility. Nine representative strains were selected from five different groups—Gram-positive non-spore-forming bacteria (Micrococcus luteus and Kocuria spp.), Gram-positive spore-forming bacteria (two Bacillus spp. strains), Gram-negative bacteria (Pseudomonas aeruginosa and Acinetobacter haemolyticus), yeasts (Candida parapsilosis and Rhodotorula mucilaginosa), and filamentous fungus (Penicillium spp.)—based on historical environmental monitoring data (2012–2022), and were characterized using matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (MALDI-TOF MS) and molecular sequencing (16S rRNA or D2 LSU rDNA). Disinfectant efficacy was assessed on stainless-steel and low-density polyethylene surfaces using NF T 72-281:2014 with adaptations, testing alcohol 70%, sodium hypochlorite 0.5%, quaternary ammonium 0.05%, peracetic acid 0.5%, and accelerated hydrogen peroxide wipes. All agents demonstrated ≥5 log₁₀ reductions against vegetative bacteria and fungi on both surfaces. However, variable sporicidal performance was observed, particularly for one Bacillus cereus group strain (B1342/15), which showed limited viability reduction on stainless steel. These findings highlight inter-strain variability and the greater tolerance of surface-associated spores. The study reinforces the importance of carrier-based testing using in-house isolates to ensure realistic validation of disinfectants and to strengthen microbiological risk management within pharmaceutical contamination control strategies. Full article

Mycoplasmas are frequently recovered from the upper respiratory tract of birds of prey, yet many isolates remain taxonomically unresolved. In the present study, a collection of ten previously unclassified Mycoplasma strains, predominantly isolated from the trachea of the common buzzard (Buteo buteo), was subjected to comprehensive phenotypic and genomic characterization. All strains grew well in modified Hayflick’s medium and formed colonies with the characteristic fried-egg appearance. None of the strains produced acid from glucose or hydrolyzed arginine or urea. Phylogenetic analyses based on 16S rRNA gene, 16S–23S intergenic spacer, and partial rpoB gene sequences placed the strains within the Mycoplasma synoviae cluster, in close proximity to five recently described Mycoplasma species associated with raptors such as eagles and kites. Matrix-assisted laser desorption ionization–time of flight (MALDI-ToF) mass spectrometry enabled the clear discrimination of the investigated strains from closely related taxa. Whole-genome comparisons, together with phylogenomic analyses, supported the assignment of these strains to a novel species within the genus Mycoplasma. The name Mycoplasma tracheobuteonis sp. nov. is proposed, corresponding to its preference for colonizing the upper respiratory tract of the common buzzard, with strain 48589B^T (=DSM 115882^T = NCTC 14927^T) designated as the type strain. Full article

Titanium alloys exhibit exceptional properties that enable their widespread application. Additive manufacturing (AM) technologies offer significant advantages for titanium alloy components, including rapid prototyping, high forming accuracy, and enhanced performance. Consequently, substantial research and industrial applications have emerged in the field of titanium alloy AM. Nevertheless, a systematic comparison and synthesis of related studies remains lacking. This paper reviews four distinct categories of titanium alloy AM processes classified by energy source (laser, electron beam, electric arc, and compressed air-assisted). Each category is analyzed in detail, with comparative assessments of microstructures, performance, and applications. Secondly, the paper comprehensively discusses current and potential applications of titanium alloy AM across aerospace, medical, and industrial sectors while identifying critical research gaps for future development. Finally, the development of novel titanium alloys for AM, titanium alloy AM assisted by acoustic or magnetic fields, and 4D printing of functional titanium alloys are discussed. Full article

Heat-assisted metal spinning comprises incremental forming routes, conventional spinning, shear spinning and flow forming, performed at elevated temperature to increase formability. This review consolidates the main advances of the last fifteen years. It outlines spinning mechanics and the rationale for heating (higher ductility, lower forming forces and microstructure control), then compares global and local heating strategies (furnace, flame, induction, laser and hot-gas convection) in terms of temperature uniformity, industrial practicality, energy efficiency and cost. Key process parameters (spindle speed, feed rate and thickness reduction) are discussed with respect to defect formation, and representative windows for defect mitigation are reported. Progress in modeling is reviewed, including coupled thermo-mechanical finite element simulations, damage/formability prediction and emerging data-driven optimization. The review also summarizes microstructural evolution under heat-assisted conditions, phase transformation, dynamic recrystallisation and grain growth, and its impact on final properties. Across more than 100 studies, evidence shows that robust thermal management can roughly double achievable deformation before failure and enables property tailoring in difficult-to-form alloys (Ni-based alloys, high-strength steels, Al, Mg and Ti). Remaining challenges include reliable in situ temperature measurement/control and improved predictive fidelity of simulations. Future opportunities include digital twins, real-time sensing and adaptive, machine-learning-assisted control. Full article

The laser-induced lift-off of functional surface layers is a key process in micro- and nano-fabrication; however, optimization criteria for maximizing the lifted-off area remain insufficiently defined. In analogy to the well-established theory of efficient laser ablation, where the maximum ablated volume per pulse is achieved at a peak fluence of $F_0^{\mathrm{o}\mathrm{p}\mathrm{t}}=e^2{F}_{\mathrm{t}\mathrm{h}}$, we develop a theoretical framework for efficient laser lift-off driven by Gaussian beams. The main highlight of this work is the derivation of a new analytical equation for the maximum delaminated area, enabling the straightforward determination of optimal processing conditions. By analytically describing the lift-off area as a function of peak fluence, beam radius, and focus position, we demonstrate that the maximum lifted-off area is achieved at a substantially lower optimal fluence, namely $F_0^{\mathrm{o}\mathrm{p}\mathrm{t}}=e^1{F}_{\mathrm{t}\mathrm{h}}$. Closed-form expressions for the optimal beam radius, maximal lift-off area, and optimal focus position are derived and validated by numerical modeling. The theory is applied to the picosecond laser lift-off of copper oxide from copper, showing excellent agreement between experimental observations and model predictions. The results reveal fundamental differences between ablation- and lift-off-dominated material removal and provide practical guidelines for maximizing process efficiency in laser-assisted delamination, selective coating removal, and surface functionalization. Full article

Aspergillosis is one of the most common fungal infections worldwide, caused by various species belonging to the genus Aspergillus, affecting both immunocompetent and immunocompromised individuals. The objective of this review was to provide an update on the last five years regarding various aspects of this mycosis, including epidemiology, risk factors, diagnosis, susceptibility, and treatment. The results showed that aspergillosis is distributed throughout the world. Furthermore, A. terreus was found to be an increasing causative agent in cases of aspergillosis, along with other less common species. Regarding clinical forms, particularly in the case of Allergic Bronchopulmonary Aspergillosis (ABPA), it is necessary to consider patients with structural lung impairment (Chronic Obstructive Pulmonary Disease (COPD) and Interstitial Lung Diseases). Meanwhile, newly identified risk factors for the development of aspergillosis include chronic obstructive pulmonary disease (odds ratio 1.88) and interstitial lung disease (OR 3.71). Furthermore, the main diagnostic methodologies for aspergillosis were polymerase chain reaction (PCR), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), and next-generation sequencing (NGS). Additionally, the usefulness of isavuconazole compared to voriconazole was demonstrated, representing a better alternative for the treatment of aspergillosis, while novel antifungals such as olorofim and fosmanogepix show excellent results in the management of aspergillosis. Due to the discovery of new risk factors, coupled with antifungal resistance in Aspergillus spp. and the wide variety of diagnostic tools, individualized assessment of aspergillosis cases is necessary for the appropriate management of this mycosis. Full article

The increasing impact of climate change and resource scarcity demands energy-efficient and resource-conserving manufacturing strategies. Metal forming offers substantial potential for lightweight construction and material efficiency. Forming-induced ductile damage, particularly void nucleation and growth, is often neglected in component design. Industrial practice still relies mainly on macroscopic mechanical properties and safety factors, while microstructural damage evolution and its influence on fatigue performance are largely disregarded. This study investigates load-path-dependent fatigue behavior and damage mechanisms using axial and combined axial–torsional fatigue tests. Particular attention is given to the phase shift d between axial and torsional loading, which strongly affects fatigue life. The results indicate that axial loading dominates damage evolution, while load path interactions significantly change fatigue performance. A phase shift of d = 90° resulted in a significant increase in the number of cycles to failure, N_f, for different total strain amplitudes with the same rotational angle amplitude of θ = 10°. These findings highlight the importance of considering load-path-sensitive stress states in fatigue assessment of formed components. Fractographic analyses, AI-assisted 3D reconstruction, and confocal laser scanning microscopy support the experimental results. Full article

Titanium and its alloys are widely used in endoprostheses. The naturally formed titanium dioxide film on titanium surfaces improves chemical stability and enhances implant biocompatibility. However, oxidised titanium surfaces may also promote bacterial adhesion and biofilm formation, contributing to implant-associated infections. Therefore, surface modification represents a key strategy for controlling microbial–implant interactions. This article focuses widely used titanium alloy Ti-6Al-4V treated with a laser beam, which induces surface colour changes as a result of oxide formation. Laser processing enables controlled formation of micro- and nanoscale features, structural reconstructions, and defects that may influence the surface electrical charge and, consequently, cell immobilisation. Thus, the surface colour, electrical potential, and cell immobilisation capacity are likely interrelated. From a manufacturing perspective, titanium oxide colouring facilitates quality control and process reproducibility, as surface colour provides a rapid, non-destructive visual indicator of oxide thickness and treatment consistency. This study aims to identify correlations among surface colour, electrical potential, and cell immobilisation capacity on laser-treated titanium alloys. A relationship between the optical properties, electronic structure, and biological response of laser-processed titanium oxide films is established. Specifically, the blue colour saturation of the oxide film is inversely correlated with the electron work function. A more saturated blue corresponds to a lower work function, indicating a higher positive surface charge density. This shift is attributed to changes in electron affinity, likely resulting from laser-induced structural reconstruction and defect formation within the oxide layer. The proposed changes in electronic structure are supported by modifications in the electronic density of states, analysed using near-threshold photoelectron spectroscopy. The biological response is directly linked to these physical changes: enhanced immobilisation of yeast (Saccharomyces cerevisiae) cells on the treated alloy surface correlates with the electron work function. These results may assist in the development of controlled titanium oxide surfaces with enhanced biocompatibility. Full article

Welding copper (Cu) and aluminum (Al) is highly demanded for lightweight and cost-effective manufacturing. However, it faces significant challenges. First, substantial differences in physical properties may lead to high residual stresses and distortion. Second, brittle intermetallic compounds (IMCs) readily form at the interface, severely compromising the joint’s mechanical properties and electrical conductivity. Third, the native oxide film on Al impedes effective wetting and bonding. Therefore, effective control over the interfacial microstructure of the welded joint is essential. This review provides a critical analysis and comparison of several typical welding techniques, including laser welding (LW), friction stir welding (FSW), ultrasonic welding (UW), brazing and soldering, and welding–brazing. These analyses focus on their process characteristics, joint microstructures, and corresponding formation mechanisms. Furthermore, this review synthesizes key strategies for enhancing joint quality, including process parameter optimization, introduction of functional interlayers, and external assistance, aimed at optimizing joint microstructure and minimizing defects. Based on the analysis, this work provides comparative insights into process selection and microstructure control, and highlights future directions: advancing novel methods such as magnetic pulse welding and transient liquid phase bonding; developing intelligent real-time process control to suppress brittle IMCs and associated defects; promoting sustainable practices and establishing standardized performance evaluation; and systematically investigating long-term reliability to support the industrial application of robust Cu/Al joints. Full article

Background/Objectives: Hospital outbreaks of Pseudomonas aeruginosa are difficult to control due to the pathogen’s extensive repertoire, including its ability to form biofilms, adapt and persist in diverse environments, and develop multidrug resistance, all of which contribute to prolonged outbreaks. This study integrates the phenotypic, proteomic, and genomic characterization of a nosocomial outbreak comprising 38 clinical isolates and one environmental isolate recovered from the intensive care unit (ICU) of Hospital IESS Quito Sur. Methods: Clinical data were collected, antimicrobial susceptibility was assessed by minimum inhibitory concentration (MIC), carbapenemase genes were detected by multiple PCR and immunochromatographic assays, and the biofilm formation index (BFI) was determined. In addition, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used for species identification and clustering based on spectral similarity. Twelve representative isolates underwent whole genome sequencing (WGS) to characterize the resistome and virulome and to compare phylogenetic relationships with proteomic clustering defined by MALDI Biotyper Compass Explorer software. Results: All isolates were identified as P. aeruginosa, and phenotypic antimicrobial susceptibility classified most isolates as multidrug resistant, including 32 CRPA strains. The bla_VIM gene was detected in 22 isolates, while BFI analysis showed that all isolates formed moderate to strong biofilms. Genomic analysis revealed that most isolates belonged to ST111 and ST253, and both conserved and heterogeneous resistome and virulome profiles, with a broad distribution of determinants related to biofilm formation, stress tolerance, and persistence. Comparison between MALDI-TOF MS and WGS showed predominant concordance in clustering, mainly within subclusters but disagreement at the cluster level. Conclusions: The detection of carbapenemases, biofilm-forming ability, and virulence determinants associated with prolonged persistence highlights the need for integrated molecular tools, such as MALDI-TOF MS with MALDI Biotyper Compass Explorer software, to support epidemiological surveillance and to inform strategies aimed at mitigating prolonged hospital outbreaks caused by P. aeruginosa. Full article

The significant disparity between the size and electronegativity of N and group-V (P, As, Sb) atoms in dilute III–V-Ns remains a cornerstone for developing the next-generation electronics. Variations in the structural, optical, and phonon properties of the quaternary GaAs_1−x−ySb_yN_x alloys are being used for improving the high-performance photovoltaic energy and optoelectronic technologies. Bandgap ${\mathrm{E}}_{\mathrm{g}}$ tunability has assisted efficient light emission/detection to cover the crucial optical fiber wavelengths for the low-cost integrated chips in data communications and sensing devices. The lattice dynamical properties of these materials are critical for assessing the reliability to evaluate the performance of long-wavelength lasers, photodetectors, and multi-junction solar cells. Our systematic Raman measurements on high-quality MBE grown $\mathrm{G}\mathrm{a}{\mathrm{A}\mathrm{s}}_{0.946}{\mathrm{S}\mathrm{b}}_{0.032}{\mathrm{N}}_{0.022}$/GaAs samples have detected ${\mathsf{\omega }}_{\mathrm{T}\mathrm{O}\left(\mathsf{\Gamma }\right)}^{\mathrm{G}\mathrm{a}\mathrm{A}\mathrm{s}}$ and ${\mathsf{\omega }}_{\mathrm{T}\mathrm{O}\left(\mathsf{\Gamma }\right)}^{\mathrm{G}\mathrm{a}\mathrm{A}\mathrm{s}}$ phonons along with a high frequency N_As local mode near ~476 cm⁻¹. Weak phonon structures on both sides of the broad 476 cm⁻¹ band are interpreted forming a complex N_As–Ga–Sb_As defect center. Using a realistic rigid-ion model in the Green’s function framework, the simulations of impurity modes for isolated and complex defects have provided corroboration to the experimental data. Full article

Basal cell carcinoma (BCC) is the most frequent skin cancer, and surgery remains the treatment of choice, particularly in high-risk subtypes and sites. However, in low-risk cases and in patients where cosmetic outcome is a priority, alternative strategies, including laser therapy, have been proposed. Different laser sources offer potential advantages in terms of minimal invasiveness, healing time, and cosmetic outcome, but their clinical role remains a matter of debate. This narrative review critically analyses the available evidence on the use of lasers in the treatment of basal cell carcinoma, with a focus on ablative lasers, vascular lasers, and laser-assisted photodynamic therapy. Mechanisms of action, main clinical results, limitations, and the emerging contribution of non-invasive imaging for case selection and response monitoring are discussed. Ablative lasers, in particular CO₂, show favourable results in superficial low-risk BCC, while clearance reliability decreases with increasing tumour depth. Vascular lasers may offer short-term control in selected lesions but with limited long-term data. Laser-assisted PDT represents a promising strategy to extend the indication of PDT to selected nodular forms. Overall, the literature is limited by methodological heterogeneity, incomplete stratification, and short follow-ups. Well-designed comparative studies, standardised protocols, and objective controls will be essential to define the real clinical space of laser therapy in basal cell carcinoma. Full article

Protein phosphorylation is a crucial post-translational modification that regulates protein activity, cellular signaling, transcriptional regulation, and cell cycle control. However, the analysis of phosphoproteins in biological samples is often compromised by complex sample matrices and interference from high-abundance proteins. While the top-down phosphoproteomics strategy enables comprehensive analysis of post-translational modifications based on intact proteins, its requirement for higher protein purity due to low protein ionization efficiency poses stern challenges. Consequently, developing appropriate enrichment methods for phosphoproteins in practical samples becomes essential. Immobilized metal ion affinity chromatography (IMAC) represents a common strategy for phosphorylated protein separation and enrichment. Among metal ions, Ti⁴⁺ has gained widespread application as IMAC chelating ligands due to its capacity to form multiple coordination networks and its high selectivity for phosphorylated protein enrichment, leveraging the strong chelating ability of phosphate groups toward metal ions. This paper presents the design and preparation of a novel magnetic Ti-IMAC nanocomposite, MNP@MPTMS–VPA–Ti(IV). The material is modified with phosphate groups via facile thiol-ene click chemistry and then immobilizes Ti⁴⁺, enabling selective enrichment of intact phosphoproteins through IMAC affinity. The efficiency of enrichment was evaluated using subsequent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for detection and analysis. This Ti-IMAC material-based magnetic solid-phase extraction (MSPE)-MALDI-TOF MS protocol has been successfully applied to enrich intact phosphoproteins in milk and eel mucus with high selectivity, sensitivity, and suitability. Full article

Legionella pneumophila (L. pneumophila), the primary causative agent of Legionnaires’ disease, is a waterborne bacterial pathogen that poses significant public health concern. This opportunistic pathogen commonly inhabits both natural and man-made water systems, particularly drinking water distribution systems (DWDSs), where it can proliferate and pose a risk to human health. In this study, we evaluated the potential of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for rapid and accurate subtyping of L. pneumophila. Our analysis included 70 L. pneumophila strains collected from the Middle East, representing one of the largest and most comprehensive MALDI-TOF MS-based subtyping of strains from this geographically underrepresented region. These strains, representing three Multi-Locus Variable Number Tandem Repeat Analysis (MLVA-8) genotypic groups (GT4, GT6, and GT15), have been extensively characterized in previous studies for their virulence traits, cytotoxicity patterns, and antimicrobial susceptibility profiles. Our findings revealed distinct genotype-associated spectral signatures with 30 discriminatory m/z peaks (p ≤ 0.005). These markers enabled accurate genotype-level classification, achieving over 85% classification accuracy with a Random Forest model and over 71% accuracy using a Decision Tree algorithm. Importantly, the m/z peak at 5358 was uniquely present in the GT15 strains, whereas m/z 5353 was consistently detected in both GT4 and GT6 isolates, demonstrating the potential of specific mass peaks to serve as reliable genotype markers. Furthermore, GT15 strains consistently formed a separate cluster in both Principal Component Analysis (PCA) and hierarchical analyses, whereas GT4 and GT6 exhibited partial overlap, reflecting their exceptionally high genomic similarity. Full article