Search Results (146)

Piriformospora indica is a beneficial endophytic fungus that promotes plant growth and root development by colonizing plant roots. In order to investigate whether P. indica could promote the growth of tissue-cultured Cerasus humilis seedlings, in this study, we co-cultivated P. indica colony segments (P+) and P. indica spore suspensions (P++) in the rooting medium, and plant biomass as well as chlorophyll and root hormone contents of ‘3-19-3’ tissue-cultured C. humilis seedlings were determined under P+, P++, and CK (without fungus inoculation) treatments. The results showed that above-ground biomass and chlorophyll content of P+-and P++-treated tissue-cultured seedlings were significantly increased, and root peroxidase (POD), indole-3-acetic-acid (IAA) content, and root activities were significantly enhanced, while jasmonic acid (JA) and 1-aminocyclopropane-1-carboxylic acid (ACC) contents were reduced. Moreover, the growth-promoting effects of P++ treatment were found to be stronger than those of P+ treatment. Our results confirmed that P. indica was able to promote the growth of tissue-cultured C. humilis seedlings and effectively promoted root development by regulating hormone content. Therefore, the application of P. indica in the production of C. humilis is promising, especially in the cultivation of elite varieties. Full article

The apple tree (Malus domestica), a member of the Rosaceae family, holds significant economic value but faces postharvest challenges, like blue mold caused by Penicillium expansum and gray mold caused by Botrytis cinerea. While synthetic fungicides are widely used, their limitations highlight the need for sustainable alternatives. This study explores the antifungal properties of extracts from Celtis australis, Olea europea var. sylvestris, Chamaerops humilis, and Asparagus albus against these pathogens. In vitro tests assessed mycelial growth inhibition, whereas in vivo trials consisted of measurement of weight loss, firmness, total soluble solids, titratable acidity, and maturity index. Moreover, the phytochemical traits of the extracts were determined using the Folin–Ciocalteu method and HPLC. The results revealed notable antifungal activity, particularly for Celtis australis extract at a concentration of 300 g L⁻¹, which led to significant mycelial growth inhibition (61% for P. expansum and 41% for B. cinerea), a reduction in diseases’ severity (39% and 50%), and a notable decrease in diseases’ incidence (43% and 48%), respectively. Phytochemical analysis reflected the presence of phenols and flavonoids in the tested extracts. Importantly, the natural treatments helped preserve the apples’ quality during storage. Molecular docking studies further revealed that major compounds in Celtis australis extract inhibit the 14α-demethylase enzyme, a key target in fungal sterols biosynthesis. Full article

The Cerasus humilis, a perennial shrub belonging to the Cerasus genus, is native to China and holds significant ecological and economic importance. Growth regulation factors (GRF) are a family of transcription factors (TF) that play a key role in plant growth and development. This research entailed an in-depth examination of the GRF family in C. humilis, exploring its significance in the evolution of C. humilis. Twelve GRF genes were identified in the C. humilis genome. Named separately as ChGRF1-Chumilis15987.1, ChGRF2-Chumilis25207.1, ChGRF3-Chumilis26233.1, ChGRF4-Chumilis08578.3, ChGRF5-Chumilis18808.1, ChGRF6-Chumilis12052.1, ChGRF7-Chumilis10417.1, ChGRF8-Chumilis01608.1, ChGRF9-Chumilis14057.1, ChGRF10-Chumilis12169.1, ChGRF11-Chumilis14952.1, and ChGRF12-Chumilis07534.1. Phylogenetic analysis divided twelve GRF genes into five subfamilies. The gene structure, pattern, and cis-regulatory components of the GRF gene family were analyzed. In addition, according to collinearity analysis, there are six collinearity with Arabidopsis, twelve collinearity with Malus pumila, eight collinearity with Vitis vinifera, and three collinearity with Oryza sativa. Intraspecific collinearity analysis revealed the presence of three pairs of tandem repeat genes in the dwarf cherry genome. Identifying cis-acting elements revealed the prominent presence of gibberellin reaction elements, which are widely distributed in the promoter region. Cluster heatmap analysis showed that ChGRF2 had the highest expression levels in fruits and stems. ChGRF3 is highly expressed in red fruits of different colors, while ChGRF6 and ChGRF12 are highly expressed in yellow fruits. This study mainly focused on dwarf cherries treated with gibberellin. As the treatment time increased, the ChGRF gene showed different expression levels. ChGRF2, ChGRF3, ChGRF6, and ChGRF12 were up-regulated under gibberellin treatment. These genes all contain hormone-responsive cis-acting elements, indicating tht the ChGRF gene family plays a vital role under gibberellin treatment in C. humilis. The results laid the foundation for further research on the biological functions of the GRF genes in C. humilis. Full article

Love and intimate relations between German men and Norwegian women were a widespread phenomenon during WWII. Like in many other European countries, these women were stigmatized and humiliated both by the authorities and by the civilian population. In this article, I discuss four postmemory literary works that address this issue: Edvard Hoem’s novel Mors og fars historie (The Story of My Mother and Father, 2005), Lene Ask’s graphic novel Hitler, Jesus og farfar (Hitler, Jesus, and Grandfather, 2006), Randi Crott and Lillian Crott Berthung’s autobiography Ikke si det til noen! (Don’t tell anyone!, 2013), and Atle Næss’s novel Blindgjengere (Duds, 2019). I explore how the narratives create a living connection between then and now and how they deal with unresolved questions and knowledge gaps. Furthermore, I discuss common themes such as the fate and identity of war children, national responsibilities versus individual choice, and norms connected to gender and sexuality. I argue that these postmemory interpretations of wartime love affairs not only aim to retell the past but to investigate the normative frameworks within which these relationships took place. My contention is that the postmemory gaze pays primary attention to the power of cultural constructions—of nationality, identity, and gender—as well as their context-related historical changes. Full article

Endemic plant species, with their restricted distribution, are vulnerable to extinction due to human activities and environmental change. Monitoring their ecological characteristics and habitat relationships is crucial for conservation. This study examined plant communities to prioritize populations for conserving the Korean endemic species, Taihyun’s abelia (Zabelia tyaihyonii (Nakai) Hisauti & H.Hara), and to identify threats and strategies for its protection. Vegetation surveys were conducted, classifying communities and analyzing species composition differences. Habitat quality and zeta diversity, assessed using the InVEST model, identified three community types: Quercus dentata–Thuja orientalis (Com. 1), Fraxinus rhynchophylla–Buxus koreana (Com. 2), and Quercus dentata–Carex humilis var. nana (Com. 3). Community classification was supported by a multi-response permutation procedure (p < 0.001) and non-metric multidimensional scaling (R² = 0.643). Species richness and soil calcium influenced species composition, and habitat quality was moderate (0.5562 ± 0.0294). Com. 1 and Com. 3 showed minimal zeta diversity decline, indicating strong habitat connectivity. However, fluctuations at zeta orders 8–12 suggested localized disturbances. Species turnover instability was linked to urbanization and disturbance. This study, using a diverse set of analytical tools, was able to pinpoint key features of habitat quality and composition associated with Z. tyaihyonii and the anthropogenic factors that will lead to its decline. Our work provides a road map for the conservation of other rare and endemic Korean plant species with similar conservation issues. Full article

Sourdough fermentation, a time-honored biotechnology known for enhancing the texture, flavor, and nutritional quality of steamed bread, has yet to be fully leveraged for optimizing microbial synergy, particularly between Kazachstania humilis (KH) and Lactobacillus plantarum (LP). In this study, we systematically evaluated the impact of fermentation dynamics on sourdough properties and steamed bread quality using single-strain (KH or LP) and co-fermentation (LP+KH) strategies. Our findings demonstrated that LP+KH co-fermentation significantly accelerated sourdough acidification, achieving the lowest pH (3.8) and highest total titratable acidity (TTA, 14.2 mL) among all groups. This synergy also enhanced dough gas retention, resulting in an 11.89% and 7.25% increase in specific volume compared to LP and KH monocultures, respectively. Steamed bread produced from the co-fermented dough exhibited markedly improved textural qualities, including reduced hardness, gumminess, and chewiness, along with increased cohesiveness. Moreover, the water content in bread from the LP+KH group remained significantly higher, contributing to better freshness retention over time. In conclusion, LP and KH co-fermentation offers a promising approach for elevating the quality and shelf-life of steamed bread, revealing untapped potential in microbial synergy during sourdough fermentation. Full article

Roasted carob flour is a sustainable ingredient rich in dietary fiber, polyphenols, and pinitol, offering potential for both food and pharmaceutical applications. However, its high sugar content and the presence of undesirable compounds such as furans present challenges for its use in bread making. This study evaluated the effects of prolonged sourdough fermentation on roasted carob flour, with a focus on microbial dynamics and its functional and technological properties. Carob and carob–wheat sourdoughs were prepared using a mixed starter culture comprising three lactic acid bacteria (Lactiplantibacillus plantarum, Fructilactobacillus sanfranciscensis, and Lactobacillus helveticus) and three yeast species (Saccharomyces cerevisiae, Kazachstania humilis, and Torulaspora delbrueckii). The sourdoughs underwent six consecutive refreshment cycles and were analyzed to determine their pH, microbial and biochemical composition, gassing power, and volatile organic compounds (VOCs). The carob–wheat sourdough exhibited faster acidification and higher lactic acid bacteria (LAB) activity, resulting in a 90–98% reduction in the sugar content, compared to 60% in the carob sourdough. Microbial sequencing revealed that L. plantarum was the dominant species in all samples, while K. humilis and S. cerevisiae were enriched in carob and carob–wheat sourdough, respectively. Both types of sourdough demonstrated effective leavening in bread dough without the addition of commercial yeast. Fermentation also modified the VOC profiles, increasing esters and alcohols while reducing acids, aldehydes, ketones, and furans. While the antioxidant activity showed a slight decline, the pinitol content remained unchanged. These findings suggest that extended sourdough fermentation, supported by multiple refreshments, enhances the baking suitability of roasted carob flour and supports its application as a functional, sustainable ingredient. Full article

Cyberbullying can be prevalent across different life stages, with lasting traces on mental health across the lifespan. This study aims to (a) explore how cyberbullying is emotionally experienced across three distinct age groups and (b) analyze the influence of cyberbullying on mental health across the lifespan. This study included 883 participants divided into three age groups: 18–39, 40–59, and 60+. In-depth semi-structured interviews were conducted to gather participants’ experiences and perspectives. The data were then subjected to content analysis, which revealed a number of themes. The first objective revealed the following themes: For ages 18–39: (a) feeling ashamed or humiliated (92.4%), (b) withdrawing from friends and family, and (c) experiencing harassment as positive and difficulties with rules. For ages 40–59: (a) losing interest in hobbies (89.5%), (b) questioning about things they did or did not do, and (c) experiencing a sense of missing out. For ages 60+: (a) negative thoughts and self-talk (91.3%), (b) feeling judged negatively, and (c) feeling financially vulnerable. The second objective showed: For 18–39: (a) depressive symptoms (79.7%), (b) easy anger, and (c) suicidal behavior. For 40–59: (a) anxiety (93.2%), (b) low self-esteem, and (c) the use of substances. For 60+: (a) frustration (78.1%), (b) isolation, and (c) disturbances in sleep and eating patterns. This study highlights the significant psychological and emotional impact of cyberbullying across age groups, emphasizing the need for targeted interventions that address the unique challenges faced by individuals at different life stages. The findings underscore the importance of developing age-specific strategies to mitigate the effects of cyberbullying and to have perpetrators take responsibility for their reckless disregard for others, and ultimately, themselves. Full article

Pinus densiflora and Quercus mongolica are representative forest vegetation communities in Baekdudaegan, South Korea. Recently, signs of deterioration, such as natural succession, disease, and insect pests, have been detected. Therefore, this study aims to classify the vegetation types and elucidate the vegetation structure across the entire South Korean section of the Baekdudaegan, from Hyangrobong to Cheonwangbong, while also proposing strategies for vegetation conservation and management. A vegetation survey was conducted in 341 plots investigated from 2016 to 2020. Cluster analysis revealed nine community types, with a single indicator species, Rhododendron schlippenbachii, in Community 1 (C1); two, Fraxinus sieboldiana and Calamagrostis arundinacea, in C2; six, including Carex humilis var. nana, Polygonatum odoratum var. pluriflorum, and Quercus variabilis, in C3; three, Sasa borealis, Q. mongolica, and Erigeron annuus, in C4; two, Rhododendron mucronulatum and Vaccinium koreanum, in C5; twelve, including Lespedeza maximowiczii, Tripterygium regelii, and Fraxinus rhynchophylla, in C6; two, Toxicodendron trichocarpum and P. densiflora, in C7; twenty, including Acer pseudosieboldianum, Acer pictum var. mono, Staphylea bumalda, and Carex pediformis, in C8; and thirteen species, including Oplismenus undulatifolius, Castanea crenata, and Smilax china, in C9. Our findings highlight the need for management plans that consider each vegetation type’s community structural characteristics. Full article

Childhood sexual abuse (CSA) is a prevalent form of violence against children, associated with profound negative impacts on survivors’ health and well-being. In Bangladesh, sociocultural and economic barriers hinder CSA prevention and intervention, particularly for vulnerable populations such as transgender individuals. This study explores the experiences of CSA among Bangladeshi transgender individuals, focusing on their coping mechanisms and resilience-building strategies. A qualitative narrative approach was employed, using life story-telling interviews with four transgender CSA survivors, and data were analyzed thematically. The findings reveal significant challenges, including social exclusion, humiliation, and lack of family support. Participants reported experiencing physical injuries, mental trauma, and loss of trust due to CSA, while the conservative nature of Bangladeshi society often prevented them from disclosing their abuse or seeking healthcare. These barriers exacerbate health inequalities among gender-minority children. Despite these adversities, participants described engaging in resilience-building strategies such as self-care, personal development, advocacy, and efforts to derive meaning from their experiences, while highlighting the perceived importance of family support. This study provides insights into the unique experiences of transgender CSA survivors in Bangladesh and calls for efforts towards gender-inclusive education, mental health support, and further research to address CSA-related adversity, encourage disclosure, and promote health equity for gender-minority groups. Full article

The genus Spiraea is well represented in the Russian flora. Several phytochemical and bioactivity studies, completed so far with several individual species of this genus, indicate young Spiraea shoots as a promising source of pharmaceutically and nutraceutically active natural products. Therefore, a broad-scale phytochemical analysis of shoot extracts from multiple Russian Spiraea species (i.e., profiling of secondary metabolites and assignment of their structures), complemented with comprehensive activity screening, might give access to valuable information on the structure–activity relationship (SAR) of their constituents. However, despite a lot of phytochemical and bioactivity information on individual species being available, these data are mostly fragmentary and do not allow for building a general picture, and in-depth comprehensive studies are still missing. Therefore, to fill this gap, here, we present a comprehensive metabolite profiling study accomplished with 15 of the most widely spread Russian Spiraea species, which was complemented with appropriate bioactivity screening of their first-year shoot alcoholic extracts. A chromatography–mass spectrometric (LC-MS) analysis revealed 33 major constituents of the shoot isolates, which were dominated by flavonoids (quercetin and kaempferol derivatives) and hydroxycinnamic acids (caffeic, ferulic, and coumaric acid derivatives). Their relative quantification indicated that most of the identified major components were distributed among all of the studied extracts with minimal overlap in their composition and relative abundance. The antioxidant activity screening revealed the high efficiency of all of the extracts as potential redox protectors, acting at the levels of radical scavenging (DPPH assay) and quenching cation radicals (TEAC assay) and superoxide anion radicals (NBT assay). Screening the antiviral and antimicrobial activity of the same extracts revealed significant antiviral activity at a concentration of 2 µg/mL, and high (MIC < 1 mg/mL) or moderate (1 mg/mL ≤ MIC ≤ 4 mg/mL) antibacterial activity against Gram-positive and Gram-negative strains. The structures responsible for the manifestation of the studied types of activity were tentatively assigned using a bioinformatics-based strategy. This analysis revealed the most bioactive Spiraea species that might be promising for further in-depth phytochemical analysis and evaluations of their structure–activity relationships (SARs). In this context, we consider S. humilis, which simultaneously showed antioxidant, antimicrobial, and antiviral activity; S. media, with marked antioxidant, antimicrobial, and cytotoxic properties; S. ussuriensis, a strong antioxidant and cytotoxic species; and S. trilobata, with a combination of antioxidant and antiviral properties. Full article

Organic acids are key components that determine the taste and flavor of fruits, playing a crucial role in maintaining fruit quality and nutritional value. To investigate the metabolic differences of organic acids between the fruit pericarp and the pulp during the developmental maturation of the Cerasus humilis, this experiment utilized Cerasus humilis cultivated in Inner Mongolia, China, as the experimental material. By measuring the malic acid and citric acid content, as well as the activities of the related metabolic enzymes in the fruit pericarp and the pulp at five developmental stages, this study investigated the characteristics of organic acid accumulation, changes in enzyme activities, and the expression trends of corresponding genes. Transcriptomic data were integrated to support the analysis. This study specifically analyzed the reasons for the differences in acidity between the pericarp and the pulp, and performed a correlation analysis of various indicators. The results indicated that, during development, the organic acid composition in both the pericarp and the pulp was primarily malic acid, with citric acid as a secondary component. The malic acid and citric acid content in the pericarp were significantly higher than in the pulp, resulting in greater overall acidity in the pericarp. The combined action of PEPC, NAD-MDH, and NADP-ME was identified as the primary reason for the differences in malic acid content between the pericarp and the pulp of Cerasus humilis. CS and ACO were identified as the key enzymes responsible for the lower citric acid content in the pulp compared to the pericarp. Furthermore, the expression levels of ChMDH2, ChME, ChCS2, ChCS3, ChACO1, and ChACO2 differed significantly between the fruit pericarp and the pulp, suggesting their regulatory roles in organic acid accumulation. Full article

Anthocyanins play a pivotal role in determining the color diversity in the flowers and fruits of Cerasus humilis (Bge.) Sok. This study performed a metabolomic analysis of the flowers and fruits of two varieties differing in pigmentation phenotypes (‘Jinou 1’ and ‘Nongda 5’), and the results indicated that the cyanidin, pelargonidin, paeonidin, and delphinidin were the main substances serving as the primary pigments contributing to their striking chromatic divergence between two varieties. Transcriptome profiling revealed that several key structural genes (ChCHS1, ChDFR, ChF3H, and ChF3’H) in the anthocyanin biosynthesis pathway exhibited significantly elevated expression levels in ’Jinou 1’ compared to ’Nongda 5’. Further metabolomic and transcriptomic correlation analyses identified that ChMYB9 and ChMYB12 exhibited strong positive associations with anthocyanin pathway metabolites in both floral and fruit tissues. Notably, ChMYB9 displayed the strongest correlation with the metabolite profiles, suggesting it may serve as a core regulatory component of the anthocyanin biosynthesis. This research provides new insights into the regulatory mechanisms of anthocyanin biosynthesis in C. humilis. Full article

This study investigates the foraging habits and dietary niche characteristics of plateau pikas (Ochotona curzoniae) by analyzing differences between the cold and warm seasons using stable isotope analysis of carbon and nitrogen in liver, muscle, and fur organs. The results indicated that in the cold season, the plant species incorporated into different organs were more diverse than in the warm season, with varying contribution rates for each plant species. Potentilla anserina made the most significant contribution to the plateau pika’s diet during the cold season, while Kobresia humilis had the highest contribution in the warm season. The trophic niche width of liver and muscle organs was broader in the cold season compared to the warm season, while the trophic niche width of the fur organ was narrower in the cold season. Additionally, long-term dietary information derived from muscle and fur organs showed a wider niche breadth compared to short-term dietary information obtained from the liver organ. Across both seasons, organ niche overlap was highest in fur, followed by muscle, and lowest in the liver. In conclusion, short-term food source information (derived from the liver organ) indicated fewer food types and a smaller niche breadth. In contrast, long-term food source information (obtained from muscle and fur organs) included a greater variety of food types and a larger niche breadth. Additionally, niche overlap was more pronounced on the long-term scale compared to the short-term scale. These findings highlight the adaptive feeding strategies of plateau pikas in response to varying seasonal food availability and the ecological importance of their dietary flexibility in maintaining energy balance in challenging environments. Full article

This study explored women living with HIV (WLHIV)’s stigma-related emotional life and sense of self in a rural Zimbabwean setting. The objective of this study was to understand the sense of stigma in the emotional lives and self-perception of women living with HIV in rural Zimbabwe. The participants were a purposive sample of 20 rural women living with HIV. Their age ranged from 20 to 65 years old. WLHIV completed semi-structured individual interviews on their emotions and sense of life. The interpretive phenomenological analysis (IPA) revealed that these rural women living with HIV endure humiliation and isolation, leading them to feeling hopeless. Their society (significant others) perceived them as burdensome social others from which little could be expected. These women experience this sense of “otherness” that represents them as social outcasts, which results in a deep sense of social isolation and loneliness, worthlessness, withdrawal, and hopelessness. The women self-perceived themselves to be constantly managing their sense of dehumanization and being stereotyped as primarily with an identity defined by disease or illness by society. The findings suggest a need for the development and implementation of support programs for building healthy self-identities for women living with HIV. Such programs would focus on strategies that counteract societal and self-stigmatization living with HIV and AIDS for full community inclusion. Full article