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Search Results (2,733)

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Keywords = human-computer interactions

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18 pages, 1388 KiB  
Review
Simulation in the Built Environment: A Bibliometric Analysis
by Saman Jamshidi
Metrics 2025, 2(3), 13; https://doi.org/10.3390/metrics2030013 - 4 Aug 2025
Abstract
Simulation has become a pivotal tool in the design, analysis, and optimization of the built environment, and has been widely adopted by professionals in architecture, engineering, and urban planning. These techniques enable stakeholders to test hypotheses, evaluate design alternatives, and predict performance outcomes [...] Read more.
Simulation has become a pivotal tool in the design, analysis, and optimization of the built environment, and has been widely adopted by professionals in architecture, engineering, and urban planning. These techniques enable stakeholders to test hypotheses, evaluate design alternatives, and predict performance outcomes prior to construction. Applications span energy consumption, airflow, thermal comfort, lighting, structural behavior, and human interactions within buildings and urban contexts. This study maps the scientific landscape of simulation research in the built environment through a bibliometric analysis of 12,220 publications indexed in Scopus. Using VOSviewer 1.6.20, it conducted citation and keyword co-occurrence analyses to identify key research themes, leading countries and journals, and central publications in the field. The analysis revealed seven primary thematic clusters: (1) human-focused simulation, (2) building-scale energy performance simulation, (3) urban-scale energy performance simulation, (4) sustainable design and simulation, (5) indoor environmental quality simulation, (6) building aerodynamics simulation, and (7) computing in building simulation. By synthesizing these trends and domains, this study provides an overview of the field, facilitating greater accessibility to the simulation literature and informing future interdisciplinary research and practice in the built environment. Full article
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13 pages, 1520 KiB  
Article
Designing a Patient Outcome Clinical Assessment Tool for Modified Rankin Scale: “You Feel the Same Way Too”
by Laura London and Noreen Kamal
Informatics 2025, 12(3), 78; https://doi.org/10.3390/informatics12030078 (registering DOI) - 4 Aug 2025
Abstract
The modified Rankin Scale (mRS) is a widely used outcome measure for assessing disability in stroke care; however, its administration is often affected by subjectivity and variability, leading to poor inter-rater reliability and inconsistent scoring. Originally designed for hospital discharge evaluations, the mRS [...] Read more.
The modified Rankin Scale (mRS) is a widely used outcome measure for assessing disability in stroke care; however, its administration is often affected by subjectivity and variability, leading to poor inter-rater reliability and inconsistent scoring. Originally designed for hospital discharge evaluations, the mRS has evolved into an outcome tool for disability assessment and clinical decision-making. Inconsistencies persist due to a lack of standardization and cognitive biases during its use. This paper presents design principles for creating a standardized clinical assessment tool (CAT) for the mRS, grounded in human–computer interaction (HCI) and cognitive engineering principles. Design principles were informed in part by an anonymous online survey conducted with clinicians across Canada to gain insights into current administration practices, opinions, and challenges of the mRS. The proposed design principles aim to reduce cognitive load, improve inter-rater reliability, and streamline the administration process of the mRS. By focusing on usability and standardization, the design principles seek to enhance scoring consistency and improve the overall reliability of clinical outcomes in stroke care and research. Developing a standardized CAT for the mRS represents a significant step toward improving the accuracy and consistency of stroke disability assessments. Future work will focus on real-world validation with healthcare stakeholders and exploring self-completed mRS assessments to further refine the tool. Full article
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45 pages, 10039 KiB  
Article
Design of an Interactive System by Combining Affective Computing Technology with Music for Stress Relief
by Chao-Ming Wang and Ching-Hsuan Lin
Electronics 2025, 14(15), 3087; https://doi.org/10.3390/electronics14153087 - 1 Aug 2025
Viewed by 179
Abstract
In response to the stress commonly experienced by young people in high-pressure daily environments, a music-based stress-relief interactive system was developed by integrating music-assisted care with emotion-sensing technology. The design principles of the system were established through a literature review on stress, music [...] Read more.
In response to the stress commonly experienced by young people in high-pressure daily environments, a music-based stress-relief interactive system was developed by integrating music-assisted care with emotion-sensing technology. The design principles of the system were established through a literature review on stress, music listening, emotion detection, and interactive devices. A prototype was created accordingly and refined through interviews with four experts and eleven users participating in a preliminary experiment. The system is grounded in a four-stage guided imagery and music framework, along with a static activity model focused on relaxation-based stress management. Emotion detection was achieved using a wearable EEG device (NeuroSky’s MindWave Mobile device) and a two-dimensional emotion model, and the emotional states were translated into visual representations using seasonal and weather metaphors. A formal experiment involving 52 users was conducted. The system was evaluated, and its effectiveness confirmed, through user interviews and questionnaire surveys, with statistical analysis conducted using SPSS 26 and AMOS 23. The findings reveal that: (1) integrating emotion sensing with music listening creates a novel and engaging interactive experience; (2) emotional states can be effectively visualized using nature-inspired metaphors, enhancing user immersion and understanding; and (3) the combination of music listening, guided imagery, and real-time emotional feedback successfully promotes emotional relaxation and increases self-awareness. Full article
(This article belongs to the Special Issue New Trends in Human-Computer Interactions for Smart Devices)
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20 pages, 980 KiB  
Article
Dynamic Decoding of VR Immersive Experience in User’s Technology-Privacy Game
by Shugang Li, Zulei Qin, Meitong Liu, Ziyi Li, Jiayi Zhang and Yanfang Wei
Systems 2025, 13(8), 638; https://doi.org/10.3390/systems13080638 (registering DOI) - 1 Aug 2025
Viewed by 170
Abstract
The formation mechanism of Virtual Reality (VR) Immersive Experience (VRIE) is notably complex; this study aimed to dynamically decode its underlying drivers by innovatively integrating Flow Theory and Privacy Calculus Theory, focusing on Perceptual-Interactive Fidelity (PIF), Consumer Willingness to Immerse in Technology (CWTI), [...] Read more.
The formation mechanism of Virtual Reality (VR) Immersive Experience (VRIE) is notably complex; this study aimed to dynamically decode its underlying drivers by innovatively integrating Flow Theory and Privacy Calculus Theory, focusing on Perceptual-Interactive Fidelity (PIF), Consumer Willingness to Immerse in Technology (CWTI), and the applicability of Loss Aversion Theory. To achieve this, we analyzed approximately 30,000 user reviews from Amazon using Latent Semantic Analysis (LSA) and regression analysis. The findings reveal that user attention’s impact on VRIE is non-linear, suggesting an optimal threshold, and confirm PIF as a central influencing mechanism; furthermore, CWTI significantly moderates users’ privacy calculus, thereby affecting VRIE, while Loss Aversion Theory showed limited explanatory power in the VR context. These results provide a deeper understanding of VR user behavior, offering significant theoretical guidance and practical implications for future VR system design, particularly in strategically balancing user cognition, PIF, privacy concerns, and individual willingness. Full article
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42 pages, 28030 KiB  
Article
Can AI and Urban Design Optimization Mitigate Cardiovascular Risks Amid Rapid Urbanization? Unveiling the Impact of Environmental Stressors on Health Resilience
by Mehdi Makvandi, Zeinab Khodabakhshi, Yige Liu, Wenjing Li and Philip F. Yuan
Sustainability 2025, 17(15), 6973; https://doi.org/10.3390/su17156973 (registering DOI) - 31 Jul 2025
Viewed by 268
Abstract
In rapidly urbanizing environments, environmental stressors—such as air pollution, noise, heat, and green space depletion—substantially exacerbate public health burdens, contributing to the global rise of non-communicable diseases, particularly hypertension, cardiovascular disorders, and mental health conditions. Despite expanding research on green spaces and health [...] Read more.
In rapidly urbanizing environments, environmental stressors—such as air pollution, noise, heat, and green space depletion—substantially exacerbate public health burdens, contributing to the global rise of non-communicable diseases, particularly hypertension, cardiovascular disorders, and mental health conditions. Despite expanding research on green spaces and health (+76.9%, 2019–2025) and optimization and algorithmic approaches (+63.7%), the compounded and synergistic impacts of these stressors remain inadequately explored or addressed within current urban planning frameworks. This study presents a Mixed Methods Systematic Review (MMSR) to investigate the potential of AI-driven urban design optimizations in mitigating these multi-scalar environmental health risks. Specifically, it explores the complex interactions between urbanization, traffic-related pollutants, green infrastructure, and architectural intelligence, identifying critical gaps in the integration of computational optimization with nature-based solutions (NBS). To empirically substantiate these theoretical insights, this study draws on longitudinal 24 h dynamic blood pressure (BP) monitoring (3–9 months), revealing that chronic exposure to environmental noise (mean 79.84 dB) increases cardiovascular risk by approximately 1.8-fold. BP data (average 132/76 mmHg), along with observed hypertensive spikes (systolic > 172 mmHg, diastolic ≤ 101 mmHg), underscore the inadequacy of current urban design strategies in mitigating health risks. Based on these findings, this paper advocates for the integration of AI-driven approaches to optimize urban environments, offering actionable recommendations for developing adaptive, human-centric, and health-responsive urban planning frameworks that enhance resilience and public health in the face of accelerating urbanization. Full article
(This article belongs to the Section Sustainable Urban and Rural Development)
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20 pages, 10161 KiB  
Article
HybridFilm: A Mixed-Reality History Tool Enabling Interoperability Between Screen Space and Immersive Environments
by Lisha Zhou, Meng Zhang, Yapeng Liu and Dongliang Guo
Appl. Sci. 2025, 15(15), 8489; https://doi.org/10.3390/app15158489 (registering DOI) - 31 Jul 2025
Viewed by 115
Abstract
History tools facilitate iterative analysis data by allowing users to view, retrieve, and revisit visualization states. However, traditional history tools are constrained by screen space limitations, which restrict the user’s ability to fully understand historical states and make it challenging to provide an [...] Read more.
History tools facilitate iterative analysis data by allowing users to view, retrieve, and revisit visualization states. However, traditional history tools are constrained by screen space limitations, which restrict the user’s ability to fully understand historical states and make it challenging to provide an intuitive preview of these states. Most immersive history tools, in contrast, operate independently of screen space and fail to consider their integration. This paper proposes HybridFilm, an innovative mixed-reality history tool that seamlessly integrates screen space and immersive reality. First, it expands the user’s understanding of historical states through a multi-source spatial fusion approach. Second, it proposes a “focus + context”-based multi-source spatial historical data visualization and interaction scheme. Furthermore, we assessed the usability and utility of HybridFilm through experimental evaluation. In comparison to traditional history tools, HybridFilm offers a more intuitive and immersive experience while maintaining a comparable level of interaction comfort and fluency. Full article
(This article belongs to the Special Issue Virtual and Augmented Reality: Theory, Methods, and Applications)
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28 pages, 3441 KiB  
Article
Which AI Sees Like Us? Investigating the Cognitive Plausibility of Language and Vision Models via Eye-Tracking in Human-Robot Interaction
by Khashayar Ghamati, Maryam Banitalebi Dehkordi and Abolfazl Zaraki
Sensors 2025, 25(15), 4687; https://doi.org/10.3390/s25154687 - 29 Jul 2025
Viewed by 334
Abstract
As large language models (LLMs) and vision–language models (VLMs) become increasingly used in robotics area, a crucial question arises: to what extent do these models replicate human-like cognitive processes, particularly within socially interactive contexts? Whilst these models demonstrate impressive multimodal reasoning and perception [...] Read more.
As large language models (LLMs) and vision–language models (VLMs) become increasingly used in robotics area, a crucial question arises: to what extent do these models replicate human-like cognitive processes, particularly within socially interactive contexts? Whilst these models demonstrate impressive multimodal reasoning and perception capabilities, their cognitive plausibility remains underexplored. In this study, we address this gap by using human visual attention as a behavioural proxy for cognition in a naturalistic human-robot interaction (HRI) scenario. Eye-tracking data were previously collected from participants engaging in social human-human interactions, providing frame-level gaze fixations as a human attentional ground truth. We then prompted a state-of-the-art VLM (LLaVA) to generate scene descriptions, which were processed by four LLMs (DeepSeek-R1-Distill-Qwen-7B, Qwen1.5-7B-Chat, LLaMA-3.1-8b-instruct, and Gemma-7b-it) to infer saliency points. Critically, we evaluated each model in both stateless and memory-augmented (short-term memory, STM) modes to assess the influence of temporal context on saliency prediction. Our results presented that whilst stateless LLaVA most closely replicates human gaze patterns, STM confers measurable benefits only for DeepSeek, whose lexical anchoring mirrors human rehearsal mechanisms. Other models exhibited degraded performance with memory due to prompt interference or limited contextual integration. This work introduces a novel, empirically grounded framework for assessing cognitive plausibility in generative models and underscores the role of short-term memory in shaping human-like visual attention in robotic systems. Full article
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19 pages, 5927 KiB  
Article
Modeling the Anti-Adhesive Role of Punicalagin Against Listeria Monocytogenes from the Analysis of the Interaction Between Internalin A and E-Cadherin
by Lorenzo Pedroni, Sergio Ghidini, Javier Vázquez, Francisco Javier Luque and Luca Dellafiora
Int. J. Mol. Sci. 2025, 26(15), 7327; https://doi.org/10.3390/ijms26157327 - 29 Jul 2025
Viewed by 259
Abstract
Listeria monocytogenes poses health threats due to its resilience and potential to cause severe infections, especially in vulnerable populations. Plant extracts and/or phytocomplexes have demonstrated the capability of natural compounds in mitigating L. monocytogenes virulence. Here we explored the suitability of a computational [...] Read more.
Listeria monocytogenes poses health threats due to its resilience and potential to cause severe infections, especially in vulnerable populations. Plant extracts and/or phytocomplexes have demonstrated the capability of natural compounds in mitigating L. monocytogenes virulence. Here we explored the suitability of a computational pipeline envisioned to identify the molecular determinants for the recognition between the bacterial protein internalin A (InlA) and the human E-cadherin (Ecad), which is the first step leading to internalization. This pipeline consists of molecular docking and extended atomistic molecular dynamics simulations to identify key interaction clusters between InlA and Ecad. It exploits this information in the screening of chemical libraries of natural compounds that might competitively interact with InIA and hence impede the formation of the InIA–Ecad complex. This strategy was effective in providing a molecular model for the anti-adhesive activity of punicalagin and disclosed two natural phenolic compounds with a similar interaction pattern. Besides elucidating key aspects of the mutual recognition between InIA and Ecad, this study provides a molecular basis about the mechanistic underpinnings of the anti-adhesive action of punicalagin that enable application against L. monocytogenes. Full article
(This article belongs to the Special Issue Computational Approaches for Protein Design)
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20 pages, 1762 KiB  
Article
EmoBERTa–CNN: Hybrid Deep Learning Approach Capturing Global Semantics and Local Features for Enhanced Emotion Recognition in Conversational Settings
by Mingfeng Zhang, Aihe Yu, Xuanyu Sheng, Jisun Park, Jongtae Rhee and Kyungeun Cho
Mathematics 2025, 13(15), 2438; https://doi.org/10.3390/math13152438 - 29 Jul 2025
Viewed by 203
Abstract
Emotion recognition in conversations is a key task in natural language processing that enhances the quality of human–computer interactions. Although existing deep learning and Transformer-based pretrained language models have shown remarkably enhanced performances, both approaches have inherent limitations. Deep learning models often fail [...] Read more.
Emotion recognition in conversations is a key task in natural language processing that enhances the quality of human–computer interactions. Although existing deep learning and Transformer-based pretrained language models have shown remarkably enhanced performances, both approaches have inherent limitations. Deep learning models often fail to capture the global semantic context, whereas Transformer-based pretrained language models can overlook subtle, local emotional cues. To overcome these challenges, we developed EmoBERTa–CNN, a hybrid framework that combines EmoBERTa’s ability to capture global semantics with the capability of convolutional neural networks (CNNs) to extract local emotional features. Experiments on the SemEval-2019 Task 3 and Multimodal EmotionLines Dataset (MELD) demonstrated that the proposed EmoBERTa–CNN model achieved F1-scores of 96.0% and 79.45%, respectively, significantly outperforming existing methods and confirming its effectiveness for emotion recognition in conversations. Full article
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16 pages, 358 KiB  
Article
Artificial Intelligence in Curriculum Design: A Data-Driven Approach to Higher Education Innovation
by Thai Son Chu and Mahfuz Ashraf
Knowledge 2025, 5(3), 14; https://doi.org/10.3390/knowledge5030014 - 29 Jul 2025
Viewed by 359
Abstract
This paper shows that artificial intelligence is fundamentally transforming college curricula by enabling data-driven personalization, which enhances student outcomes and better aligns educational programs with evolving workforce demands. Specifically, predictive analytics, machine learning algorithms, and natural language processing were applied here, grounded in [...] Read more.
This paper shows that artificial intelligence is fundamentally transforming college curricula by enabling data-driven personalization, which enhances student outcomes and better aligns educational programs with evolving workforce demands. Specifically, predictive analytics, machine learning algorithms, and natural language processing were applied here, grounded in constructivist learning theory and Human–Computer Interaction principles, to evaluate student performance and identify at-risk students to propose personalized learning pathways. Results indicated that the AI-based curriculum achieved much higher course completion rates (89.72%) as well as retention (91.44%) and dropout rates (4.98%) compared to the traditional model. Sentiment analysis of learner feedback showed a more positive learning experience, while regression and ANOVA analyses proved the impact of AI on enhancing academic performance to be real. Therefore, the learning content delivery for each student was continuously improved based on individual learner characteristics and industry trends by AI-enabled recommender systems and adaptive learning models. Its advantages notwithstanding, the study emphasizes the need to address ethical concerns, ensure data privacy safeguards, and mitigate algorithmic bias before an equitable outcome can be claimed. These findings can inform institutions aspiring to adopt AI-driven models for curriculum innovation to build a more dynamic, responsive, and learner-centered educational ecosystem. Full article
(This article belongs to the Special Issue Knowledge Management in Learning and Education)
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23 pages, 19710 KiB  
Article
Hybrid EEG Feature Learning Method for Cross-Session Human Mental Attention State Classification
by Xu Chen, Xingtong Bao, Kailun Jitian, Ruihan Li, Li Zhu and Wanzeng Kong
Brain Sci. 2025, 15(8), 805; https://doi.org/10.3390/brainsci15080805 - 28 Jul 2025
Viewed by 235
Abstract
Background: Decoding mental attention states from electroencephalogram (EEG) signals is crucial for numerous applications such as cognitive monitoring, adaptive human–computer interaction, and brain–computer interfaces (BCIs). However, conventional EEG-based approaches often focus on channel-wise processing and are limited to intra-session or subject-specific scenarios, lacking [...] Read more.
Background: Decoding mental attention states from electroencephalogram (EEG) signals is crucial for numerous applications such as cognitive monitoring, adaptive human–computer interaction, and brain–computer interfaces (BCIs). However, conventional EEG-based approaches often focus on channel-wise processing and are limited to intra-session or subject-specific scenarios, lacking robustness in cross-session or inter-subject conditions. Methods: In this study, we propose a hybrid feature learning framework for robust classification of mental attention states, including focused, unfocused, and drowsy conditions, across both sessions and individuals. Our method integrates preprocessing, feature extraction, feature selection, and classification in a unified pipeline. We extract channel-wise spectral features using short-time Fourier transform (STFT) and further incorporate both functional and structural connectivity features to capture inter-regional interactions in the brain. A two-stage feature selection strategy, combining correlation-based filtering and random forest ranking, is adopted to enhance feature relevance and reduce dimensionality. Support vector machine (SVM) is employed for final classification due to its efficiency and generalization capability. Results: Experimental results on two cross-session and inter-subject EEG datasets demonstrate that our approach achieves classification accuracy of 86.27% and 94.01%, respectively, significantly outperforming traditional methods. Conclusions: These findings suggest that integrating connectivity-aware features with spectral analysis can enhance the generalizability of attention decoding models. The proposed framework provides a promising foundation for the development of practical EEG-based systems for continuous mental state monitoring and adaptive BCIs in real-world environments. Full article
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24 pages, 10460 KiB  
Article
WGGLFA: Wavelet-Guided Global–Local Feature Aggregation Network for Facial Expression Recognition
by Kaile Dong, Xi Li, Cong Zhang, Zhenhua Xiao and Runpu Nie
Biomimetics 2025, 10(8), 495; https://doi.org/10.3390/biomimetics10080495 - 27 Jul 2025
Viewed by 296
Abstract
Facial expression plays an important role in human–computer interaction and affective computing. However, existing expression recognition methods cannot effectively capture multi-scale structural details contained in facial expressions, leading to a decline in recognition accuracy. Inspired by the multi-scale processing mechanism of the biological [...] Read more.
Facial expression plays an important role in human–computer interaction and affective computing. However, existing expression recognition methods cannot effectively capture multi-scale structural details contained in facial expressions, leading to a decline in recognition accuracy. Inspired by the multi-scale processing mechanism of the biological visual system, this paper proposes a wavelet-guided global–local feature aggregation network (WGGLFA) for facial expression recognition (FER). Our WGGLFA network consists of three main modules: the scale-aware expansion (SAE) module, which combines dilated convolution and wavelet transform to capture multi-scale contextual features; the structured local feature aggregation (SLFA) module based on facial keypoints to extract structured local features; and the expression-guided region refinement (ExGR) module, which enhances features from high-response expression areas to improve the collaborative modeling between local details and key expression regions. All three modules utilize the spatial frequency locality of the wavelet transform to achieve high-/low-frequency feature separation, thereby enhancing fine-grained expression representation under frequency domain guidance. Experimental results show that our WGGLFA achieves accuracies of 90.32%, 91.24%, and 71.90% on the RAF-DB, FERPlus, and FED-RO datasets, respectively, demonstrating that our WGGLFA is effective and has more capability of robustness and generalization than state-of-the-art (SOTA) expression recognition methods. Full article
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17 pages, 8512 KiB  
Article
Interactive Holographic Display System Based on Emotional Adaptability and CCNN-PCG
by Yu Zhao, Zhong Xu, Ting-Yu Zhang, Meng Xie, Bing Han and Ye Liu
Electronics 2025, 14(15), 2981; https://doi.org/10.3390/electronics14152981 - 26 Jul 2025
Viewed by 299
Abstract
Against the backdrop of the rapid advancement of intelligent speech interaction and holographic display technologies, this paper introduces an interactive holographic display system. This paper applies 2D-to-3D technology to acquisition work and uses a Complex-valued Convolutional Neural Network Point Cloud Gridding (CCNN-PCG) algorithm [...] Read more.
Against the backdrop of the rapid advancement of intelligent speech interaction and holographic display technologies, this paper introduces an interactive holographic display system. This paper applies 2D-to-3D technology to acquisition work and uses a Complex-valued Convolutional Neural Network Point Cloud Gridding (CCNN-PCG) algorithm to generate a computer-generated hologram (CGH) with depth information for application in point cloud data. During digital human hologram building, 2D-to-3D conversion yields high-precision point cloud data. The system uses ChatGLM for natural language processing and emotion-adaptive responses, enabling multi-turn voice dialogs and text-driven model generation. The CCNN-PCG algorithm reduces computational complexity and improves display quality. Simulations and experiments show that CCNN-PCG enhances reconstruction quality and speeds up computation by over 2.2 times. This research provides a theoretical framework and practical technology for holographic interactive systems, applicable in virtual assistants, educational displays, and other fields. Full article
(This article belongs to the Special Issue Artificial Intelligence, Computer Vision and 3D Display)
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20 pages, 16450 KiB  
Article
A Smart Textile-Based Tactile Sensing System for Multi-Channel Sign Language Recognition
by Keran Chen, Longnan Li, Qinyao Peng, Mengyuan He, Liyun Ma, Xinxin Li and Zhenyu Lu
Sensors 2025, 25(15), 4602; https://doi.org/10.3390/s25154602 - 25 Jul 2025
Viewed by 289
Abstract
Sign language recognition plays a crucial role in enabling communication for deaf individuals, yet current methods face limitations such as sensitivity to lighting conditions, occlusions, and lack of adaptability in diverse environments. This study presents a wearable multi-channel tactile sensing system based on [...] Read more.
Sign language recognition plays a crucial role in enabling communication for deaf individuals, yet current methods face limitations such as sensitivity to lighting conditions, occlusions, and lack of adaptability in diverse environments. This study presents a wearable multi-channel tactile sensing system based on smart textiles, designed to capture subtle wrist and finger motions for static sign language recognition. The system leverages triboelectric yarns sewn into gloves and sleeves to construct a skin-conformal tactile sensor array, capable of detecting biomechanical interactions through contact and deformation. Unlike vision-based approaches, the proposed sensor platform operates independently of environmental lighting or occlusions, offering reliable performance in diverse conditions. Experimental validation on American Sign Language letter gestures demonstrates that the proposed system achieves high signal clarity after customized filtering, leading to a classification accuracy of 94.66%. Experimental results show effective recognition of complex gestures, highlighting the system’s potential for broader applications in human-computer interaction. Full article
(This article belongs to the Special Issue Advanced Tactile Sensors: Design and Applications)
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16 pages, 2230 KiB  
Article
Three-Dimensional-Printed Biomimetic Scaffolds for Investigating Osteoblast-Like Cell Interactions in Simulated Microgravity: An In Vitro Platform for Bone Tissue Engineering Research
by Eleonora Zenobi, Giulia Gramigna, Elisa Scatena, Luca Panizza, Carlotta Achille, Raffaella Pecci, Annalisa Convertino, Costantino Del Gaudio, Antonella Lisi and Mario Ledda
J. Funct. Biomater. 2025, 16(8), 271; https://doi.org/10.3390/jfb16080271 - 24 Jul 2025
Viewed by 605
Abstract
Three-dimensional cell culture systems are relevant in vitro models for studying cellular behavior. In this regard, this present study investigates the interaction between human osteoblast-like cells and 3D-printed scaffolds mimicking physiological and osteoporotic bone structures under simulated microgravity conditions. The objective is to [...] Read more.
Three-dimensional cell culture systems are relevant in vitro models for studying cellular behavior. In this regard, this present study investigates the interaction between human osteoblast-like cells and 3D-printed scaffolds mimicking physiological and osteoporotic bone structures under simulated microgravity conditions. The objective is to assess the effects of scaffold architecture and dynamic culture conditions on cell adhesion, proliferation, and metabolic activity, with implications for osteoporosis research. Polylactic acid scaffolds with physiological (P) and osteoporotic-like (O) trabecular architectures were 3D-printed by means of fused deposition modeling technology. Morphometric characterization was performed using micro-computed tomography. Human osteoblast-like SAOS-2 and U2OS cells were cultured on the scaffolds under static and dynamic simulated microgravity conditions using a rotary cell culture system (RCCS). Scaffold biocompatibility, cell viability, adhesion, and metabolic activity were evaluated through Bromodeoxyuridine incorporation assays, a water-soluble tetrazolium salt assay, and an enzyme-linked immunosorbent assay of tumor necrosis factor-α secretion. Both scaffold models supported osteoblast-like cell adhesion and growth, with an approximately threefold increase in colonization observed on the high-porosity O scaffolds under dynamic conditions. The dynamic environment facilitated increased surface interaction, amplifying the effects of scaffold architecture on cell behavior. Overall, sustained cell growth and metabolic activity, together with the absence of detectable inflammatory responses, confirmed the biocompatibility of the system. Scaffold microstructure and dynamic culture conditions significantly influence osteoblast-like cell behavior. The combination of 3D-printed scaffolds and a RCCS bioreactor provides a promising platform for studying bone remodeling in osteoporosis and microgravity-induced bone loss. These findings may contribute to the development of advanced in vitro models for biomedical research and potential countermeasures for bone degeneration. Full article
(This article belongs to the Special Issue Functional Biomaterial for Bone Regeneration)
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