Search Results (796)

Spiking neural networks (SNNs) have emerged as a promising model for energy-efficient, event-driven processing of asynchronous event streams from Dynamic Vision Sensors (DVSs), a class of neuromorphic image sensors with microsecond-level latency and high dynamic range. Nevertheless, challenges persist in optimising training and effectively handling spatio-temporal complexity, which limits their potential for real-time applications on embedded sensing systems such as object tracking and recognition. Targeting this neuromorphic sensing pipeline, this paper proposes the Dynamic Tracking with Event Attention Spiking Network (DTEASN), a novel framework designed to address these challenges by employing a pure SNN architecture, bypassing conventional convolutional neural network (CNN) operations, and reducing GPU resource dependency, while tailoring the processing to DVS signal characteristics (asynchrony, sparsity, and polarity). The model incorporates two innovative, self-developed components: an event-driven multi-scale attention mechanism and a spatio-temporal event convolver, both of which significantly enhance spatio-temporal feature extraction from raw DVS events. An Event-Weighted Spiking Loss (EW-SLoss) is introduced to optimise the learning process by prioritising informative events and improving robustness to sensor noise. Additionally, a lightweight event tracking mechanism and a custom synaptic connection rule are proposed to further improve model efficiency for low-power, edge deployment. The efficacy of DTEASN is demonstrated through empirical results on event-based (DVS) object recognition and tracking benchmarks, where it outperforms conventional methods in accuracy, latency, event throughput (events/s) and spike rate (spikes/s), memory footprint, spike-efficiency (energy proxy), and overall computational efficiency under typical DVS settings. By virtue of its event-aligned, sparse computation, the framework is amenable to highly parallel neuromorphic hardware, supporting on- or near-sensor inference for embedded applications. Full article

Caenorhabditis elegans is one of the most extensively studied model organisms in biology. Its advantageous features, including genetic homology with humans, conservation of disease pathways, transparency, short lifespan, small size and ease of maintenance have established it as a powerful system for research in aging, genetics, molecular biology, disease modeling and drug discovery. However, traditional methods for worm handling, culturing, scoring and imaging are labor-intensive, low throughput, time consuming, susceptible to operator variability and environmental influences. Addressing these challenges, recent years have seen rapid innovation spanning microfluidics, robotics, imaging platforms and AI-driven analysis in C. elegans-based research. Advances include micromanipulation devices, robotic microinjection systems, automated worm assays and high-throughput screening platforms. In this review, we first summarize foundational developments prior to 2020 that shaped the field, then highlight breakthroughs from the past five years that address key limitations in throughput, reproducibility and scalability. Finally, we discuss ongoing challenges and future directions for integrating these technologies into next-generation automated C. elegans research. Full article

Organismal biosensing leverages the olfactory acuity of living systems to detect volatile organic compounds (VOCs) associated with cancer, offering a low-cost and non-invasive complement to conventional diagnostics. Early studies demonstrate its feasibility across diverse platforms. In C. elegans, chemotaxis assays on urine samples achieved sensitivities of 87–96% and specificities of 90–95% in case–control cohorts (n up to 242), while calcium imaging of AWC neurons distinguished breast cancer urine with ~97% accuracy in a small pilot cohort (n ≈ 40). Trained canines have identified prostate cancer from urine with sensitivities of ~71% and specificities of 70–76% (n ≈ 50), and AI-augmented canine breath platforms have reported accuracies of ~94–95% across ~1400 participants. Insects such as locusts and honeybees enable ultrafast neural decoding of VOCs, achieving 82–100% classification accuracy within 250 ms in pilot studies (n ≈ 20–30). Collectively, these platforms validate the principle that organismal behavior and neural activity encode cancer-related VOC signatures. However, limitations remain, including small cohorts, methodological heterogeneity, and reliance on binary outputs. This review proposes a Dual-Pathway Framework, where Pathway 1 leverages validated indices (e.g., the Chemotaxis Index) for high-throughput screening, and Pathway 2 applies machine learning to high-dimensional behavioral vectors for cancer subtyping, staging, and monitoring. By integrating these approaches, organismal biosensing could evolve from proof-of-concept assays into clinically scalable precision diagnostics. Full article

The burgeoning Internet of Medical Things (IoMT) offers unprecedented opportunities for real-time patient monitoring and predictive diagnostics, yet the current systems struggle with scalability, data confidentiality against quantum threats, and real-time privacy-preserving intelligence. This paper introduces Med-Q Ledger, a novel, multi-layered framework designed to overcome these critical limitations in the Medical IoT domain. Med-Q Ledger integrates a permissioned Hyperledger Fabric for transactional integrity with a scalable Holochain Distributed Hash Table for high-volume telemetry, achieving horizontal scalability and sub-second commit times. To fortify long-term data security, the framework incorporates post-quantum cryptography (PQC), specifically CRYSTALS-Di lithium signatures and Kyber Key Encapsulation Mechanisms. Real-time, privacy-preserving intelligence is delivered through an edge-based federated learning (FL) model, utilizing lightweight autoencoders for anomaly detection on encrypted gradients. We validate Med-Q Ledger’s efficacy through a critical application: the prediction of intestinal complications like necrotizing enterocolitis (NEC) in preterm infants, a condition frequently necessitating emergency colostomy. By processing physiological data from maternal wearable sensors and infant intestinal images, our integrated Random Forest model demonstrates superior performance in predicting colostomy necessity. Experimental evaluations reveal a throughput of approximately 3400 transactions per second (TPS) with ~180 ms end-to-end latency, a >95% anomaly detection rate with <2% false positives, and an 11% computational overhead for PQC on resource-constrained devices. Furthermore, our results show a 0.90 F1-score for colostomy prediction, a 25% reduction in emergency surgeries, and 31% lower energy consumption compared to MQTT baselines. Med-Q Ledger sets a new benchmark for secure, high-performance, and privacy-preserving IoMT analytics, offering a robust blueprint for next-generation healthcare deployments. Full article

Mechanical exfoliation remains the most common method for producing high-quality two-dimensional (2D) materials, but its inherently low yield requires screening large numbers of samples to identify usable flakes. Efficient optimization of the exfoliation process demands scalable methods to analyze deposited material across extensive datasets. While machine learning clustering techniques have demonstrated ~95% accuracy in classifying 2D material thicknesses from optical microscopy images, current tools are limited by slow processing speeds and heavy reliance on manual user input. This work presents an open-source, GPU-accelerated software platform that builds upon existing classification methods to enable high-throughput analysis of 2D material samples. By leveraging parallel computation, optimizing core algorithms, and automating preprocessing steps, the software can quantify flake coverage and thickness across uncompressed optical images at scale. Benchmark comparisons show that this implementation processes over 200× more pixel data with a 60× reduction in processing time relative to the original software. Specifically, a full dataset of2916 uncompressed images can be classified in 35 min, compared to an estimated 32 h required by the baseline method using compressed images. This platform enables rapid evaluation of exfoliation results across multiple trials, providing a practical tool for optimizing deposition techniques and improving the yield of high-quality 2D materials. Full article

Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. It is characterized by a high degree of heterogeneity, meaning that although these tumors may appear morphologically similar, they often exhibit distinct clinical outcomes. By associating specific molecular fingerprints with different clinical behaviors, high-throughput omics technologies (e.g., genomics, transcriptomics, and epigenomics) have significantly advanced our understanding of GBM, particularly of its extensive heterogeneity, by proposing a molecular classification for the implementation of precision medicine. However, due to the vast volume and complexity of data, the integrative analysis of omics data demands substantial computational power for processing, analyzing and interpreting GBM-related data. Artificial intelligence (AI), which mainly includes machine learning (ML) and deep learning (DL) computational approaches, now presents a unique opportunity to infer valuable biological insights from omics data and enhance the clinical management of GBM. In this review, we explored the potential of integrating multi-omics, imaging radiomics and clinical data with AI to uncover different aspects of GBM (molecular profiling, prognosis, and treatment) and improve its clinical management. Full article

Insights into the state of individual cells within a living organism are essential for identifying diseases and abnormalities. The internal state of a cell is reflected in its morphological features and changes in the localization of intracellular molecules. Using this information, it is possible to infer the state of the cells with high precision. In recent years, technological advancements and improvements in instrument specifications have made large-scale analyses, such as single-cell analysis, more widely accessible. Among these technologies, imaging flow cytometry (IFC) is a high-throughput imaging platform that can simultaneously acquire information from flow cytometry (FCM) and cellular images. While conventional FCM can only obtain fluorescence intensity information corresponding to each detector, IFC can acquire multidimensional information, including cellular morphology and the spatial arrangement of proteins, nucleic acids, and organelles for each imaging channel. This enables the discrimination of cell types and states based on the localization of proteins and organelles, which is difficult to assess accurately using conventional FCM. Because IFC can acquire a large number of single-cell morphological images in a short time, it is well suited for automated classification using machine learning. Furthermore, commercial instruments that combine integrated imaging and cell sorting capabilities have recently become available, enabling the sorting of cells based on their image information. In this review, we specifically highlight practical applications of IFC in four representative areas: cell cycle analysis, protein localization analysis, immunological synapse formation, and the detection of leukemic cells. In addition, particular emphasis is placed on applications that directly contribute to elucidating molecular mechanisms, thereby distinguishing this review from previous general overviews of IFC. IFC enables the estimation of cell cycle phases from large numbers of acquired cellular images using machine learning, thereby allowing more precise cell cycle analysis. Moreover, IFC has been applied to investigate intracellular survival and differentiation signals triggered by external stimuli, to monitor DNA damage responses such as γH2AX foci formation, and more recently, to detect immune synapse formation among interacting cells within large populations and to analyze these interactions at the molecular level. In hematological malignancies, IFC combined with fluorescence in situ hybridization (FISH) enables high-throughput detection of chromosomal abnormalities, such as BCR-ABL1 translocations. These advances demonstrate that IFC provides not only morphological and functional insights but also clinically relevant genomic information at the single-cell level. By summarizing these unique applications, this review aims to complement existing publications and provide researchers with practical insights into how IFC can be implemented in both basic and translational research. Full article

Achieving rapid and accurate object classification holds significant importance in various domains. However, conventional vision-based techniques suffer from several limitations, including high data redundancy and strong dependence on image quality. In this work, we present a high-speed, image-free object classification method based on dual-pixel measurement and normalized central moment invariants. Leveraging the complementary modulation capability of a digital micromirror device (DMD), the proposed system requires only five tailored binary illumination patterns to simultaneously extract geometric features and perform classification. The system can achieve a classification update rate of up to 4.44 kHz, offering significant improvements in both efficiency and accuracy compared to traditional image-based approaches. Numerical simulations verify the robustness of the method under similarity transformations—including translation, scaling, and rotation—while experimental validations further demonstrate reliable performance across diverse object types. This approach enables real-time, low-data throughput, and reconstruction-free classification, offering new potential for optical computing and edge intelligence applications. Full article

Neuronal chloride (Cl⁻) homeostasis is fundamental for brain function, with disruptions increasingly recognized as pathogenic across neurological disorders. This review synthesizes evidence from preclinical models and clinical studies, integrating electrophysiological measurements, molecular analyses, imaging with genetically encoded sensors like ClopHensor, and behavioral assays. Key findings demonstrate that Cl⁻ dysregulation follows distinct patterns: (1) in epilepsy, KCC2 downregulation converts GABAergic inhibition to excitation, promoting seizures; (2) in Alzheimer’s disease (AD) models, pre-symptomatic KCC2 loss in hippocampus is observed, with KCC2 restoration reversing aspects of cognitive decline; (3) in autism spectrum disorders (ASD), developmental delays in GABA polarity shifts feature due to altered NKCC1/KCC2 ratios; and (4) in Huntington’s disease (HD), striatal neuron-specific Cl⁻ imbalances are linked to motor dysfunction. Methodologically, advanced tools—including subcellular Cl⁻ imaging and high-throughput drug screening—have enabled precise dissection of these mechanisms. Therapeutic strategies targeting Cl⁻ transporters (NKCC1 inhibitors like bumetanide, KCC2 enhancers like CLP290) show preclinical promise but require improved central nervous system (CNS) delivery and selectivity. These findings establish Cl⁻ homeostasis as both a biomarker and therapeutic target, necessitating precision medicine approaches to address heterogeneity in neurological disorders. Full article

Micro-X-ray fluorescence spectroscopy (micro-XRF) integrates spatial and spectral information and is widely employed for multi-elemental analyses of rock-forming minerals. However, its inherent limitation in spatial resolution gives rise to significant pixel mixing, thereby hindering the accurate identification of fine-scale or anomalous mineral phases. Furthermore, most existing methods heavily rely on manually labeled data or predefined spectral libraries, rendering them poorly adaptable to complex and variable mineral systems. To address these challenges, this paper presents an unsupervised deep aggregation network (MSFA-Net) for micro-XRF imagery, aiming to eliminate the reliance of traditional methods on prior knowledge and enhance the recognition capability of rare mineral anomalies. Built on an autoencoder architecture, MSFA-Net incorporates a multi-scale orthogonal attention module to strengthen spectral–spatial feature fusion and employs density-based adaptive clustering to guide semantically aware reconstruction, thus achieving high-precision responses to potential anomalous regions. Experiments on real-world micro-XRF datasets demonstrate that MSFA-Net not only outperforms mainstream anomaly detection methods but also transcends the physical resolution limits of the instrument, successfully identifying subtle mineral anomalies that traditional approaches fail to detect. This method presents a novel paradigm for high-throughput and weakly supervised interpretation of complex geological images. Full article

Microplastics (MPs) pollution has become a global environmental issue. Soil, as a key environmental medium, serves as an important sink and carrier of MPs. Accurate and efficient extraction of MPs from soil matrices is essential for understanding their distribution, composition, and environmental behavior. This study presents a refined extraction method that combines two-step density separation with sodium chloride (NaCl, 1.20 g/cm³), hydrogen peroxide (H₂O₂) digestion for organic matter removal and a Fractionated Filtration Method (FFM) to capture MPs across multiple particle size ranges. Polymer identification and size characterization were performed using the high-throughput Agilent 8700 Laser Direct Infrared (LDIR) imaging system. Method validation demonstrated a recovery rate of 85% based on 100 μm MPs standards spiked into soil and minimal background contamination of 5–8 particles in blank controls, confirming the reliability of the workflow. Applying this method to agricultural soils from the Hetao Irrigation District revealed widespread MP contamination, with concentrations ranging from 5778 to 31,489 particles/kg and an average of 16,461 ± 8097 particles/kg. More than 99% of MPs were smaller than 500 μm, with the 10–30 μm fraction dominating the distribution. Polypropylene (PP), polyamide (PA), and polyethylene (PE) accounted for over 90% of detected MPs. This refined method enables reproducible extraction and accurate characterization of fine MPs in complex soil environments and provides a practical foundation for advancing standardized soil MP monitoring protocols. Full article

The exponential growth of AI applications has intensified the demand for efficient inference hardware capable of delivering low-latency, high-throughput, and energy-efficient performance. This study presents a systematic, empirical comparison of GPU- and NPU-based server platforms across key AI inference domains: text-to-text, text-to-image, multimodal understanding, and object detection. We configure representative models—LLama-family for text generation, Stable Diffusion variants for image synthesis, LLaVA-NeXT for multimodal tasks, and YOLO11 series for object detection—on a dual NVIDIA A100 GPU server and an eight-chip RBLN-CA12 NPU server. Performance metrics including latency, throughput, power consumption, and energy efficiency are measured under realistic workloads. Results demonstrate that NPUs match or exceed GPU throughput in many inference scenarios while consuming 35–70% less power. Moreover, optimization with the vLLM library on NPUs nearly doubles the tokens-per-second and yields a 92% increase in power efficiency. Our findings validate the potential of NPU-based inference architectures to reduce operational costs and energy footprints, offering a viable alternative to the prevailing GPU-dominated paradigm. Full article

In situ detection of growth information in greenhouse crops is crucial for germplasm resource optimization and intelligent greenhouse management. To address the limitations of poor flexibility and low automation in traditional phenotyping platforms, this study developed a controlled environment inspection robot. By means of a SCARA robotic arm equipped with an information acquisition device consisting of an RGB camera, a depth camera, and an infrared thermal imager, high-throughput and in situ acquisition of lettuce phenotypic information can be achieved. Through semantic segmentation and point cloud reconstruction, 12 phenotypic parameters, such as lettuce plant height and crown width, were extracted from the acquired images as inputs for three machine learning models to predict fresh weight. By analyzing the training results, a Backpropagation Neural Network (BPNN) with an added feature dimension-increasing module (DE-BP) was proposed, achieving improved prediction accuracy. The R² values for plant height, crown width, and fresh weight predictions were 0.85, 0.93, and 0.84, respectively, with RMSE values of 7 mm, 6 mm, and 8 g, respectively. This study achieved in situ, high-throughput acquisition of lettuce phenotypic information under controlled environmental conditions, providing a lightweight solution for crop phenotypic information analysis algorithms tailored for inspection tasks. Full article

Smart logistics systems generate massive amounts of data, such as images and videos, requiring real-time processing in edge clusters. However, the edge cluster systems face performance bottlenecks in reception and forwarding high-concurrency data streams from numerous smart terminals, resulting in degraded processing efficiency. To address this issue, a novel high-performance data stream model called CBPS-DPDK is proposed. CBPS-DPDK integrates the DPDK framework from Intel corporations with a content-based publish/subscribe model enhanced by semantic filtering. This model adopts a three-tier optimization architecture. First, the user-space data plane is restructured using DPDK to avoid kernel context switch overhead via zero-copy and polling. Second, semantic enhancement is introduced into the publish/subscribe model to reduce the coupling between data producers and consumers through subscription matching and priority queuing. Finally, a hierarchical load balancing strategy ensures reliable data transmission under high concurrency. Experimental results show that CBPS-DPDK significantly outperforms two baselines—OSKT (kernel-based data forwarding) and DPDK-only (DPDK). Relative to the OSKT baseline, DPDK-only achieves improvements of 37.5% in latency, 11.1% in throughput, and 9.1% in VMAF; CBPS-DPDK further increases these to 51.8%, 18.3%, and 11.2%, respectively. In addition, compared with the traditional publish–subscribe system NATS, CBPS-DPDK maintains lower delay, higher throughput, and more balanced CPU and memory utilization under saturated workloads, demonstrating its effectiveness for real-time, high-concurrency edge scenarios. Full article

Black fungus cultivation in bagged form requires frequent inspection of mycelial growth, a process that is labor-intensive and susceptible to subjective judgment. In addition, timely detection of contamination in low-light and high-humidity environments remains a significant challenge. To address these issues, this paper proposed an intelligent agriculture system for black fungus cultivation, with emphasis on practical deployment under real farming conditions. The system integrates a private 5G network with a YOLOv8-based deep learning model for real-time object detection and growth monitoring. Continuous image acquisition and data feedback are achieved through a multi-parameter environmental sensing module and an autonomous ground vehicle (AGV) equipped with IP cameras. To improve model robustness, more than 42,000 labeled training images were generated through data augmentation, and a modified C2f network architecture was employed. Experimental results show that the model achieved a detection accuracy of 93.7% with an average confidence score of 0.96 under live testing conditions. The deployed 5G network provided a downlink throughput of 645.2 Mbps and an uplink throughput of 147.5 Mbps, ensuring sufficient bandwidth and low latency for real-time inference and transmission. Field trials conducted over five cultivation batches demonstrated improvements in disease detection, reductions in labor requirements, and an increase in the average yield success rate to 80%. These findings indicate that the proposed method offers a scalable and practical solution for precision agriculture, integrating next-generation communication technologies with deep learning to enhance cultivation management. Full article