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20 pages, 1778 KB  
Article
Aiding Imperiled Fish and Mussel Conservation Using Swimming Performance Metrics to Inform the Design or Modification of Road Stream Crossings
by Allie N. Burdette-Lapuz, Malachi R. Hubbard, Cameron M. Emadi, Preston T. Bean and Edward M. Mager
Fishes 2026, 11(8), 488; https://doi.org/10.3390/fishes11080488 - 19 Aug 2026
Viewed by 120
Abstract
Culvert construction at stream crossing sites can lead to zones of high-velocity water flow which can disrupt the dispersal and connectivity of species of greatest conservation need (SGCN) in Texas. To help inform effective culvert design, the swimming performance of the following seven [...] Read more.
Culvert construction at stream crossing sites can lead to zones of high-velocity water flow which can disrupt the dispersal and connectivity of species of greatest conservation need (SGCN) in Texas. To help inform effective culvert design, the swimming performance of the following seven fish species has been measured, the first three of which are SGCN themselves, and the last four serve as hosts for glochidia of SGCN mussels (mussel genera indicated in parentheses): Colorado Roundnose Minnow, Texas Shiner, Headwater Catfish, Largemouth Bass (host for Lampsilis spp.), Green Sunfish (host for Lampsilis spp.), Channel Catfish (host for Cyclonaias spp.) and Blacktail Shiner (host for Fusconaia spp.). The primary objective was to measure the critical swimming speeds (Ucrit) under a range of relevant temperatures (15, 22.5, and 30 °C) to be used in site-specific calculations of culvert water velocities (Vf). A secondary objective was to collect additional physiological endpoints of relevance to overall swimming performance, including maximum burst swimming speed (Umax), metabolic rate measurements (i.e., standard (SMR), maximum (MMR) and aerobic scope (AS)) and calculations of cost of transport (COT) and optimal swimming speed (Uopt). As expected, Ucrit tended to increase with increasing temperature for several species with metabolic rate measurements following congruent trends, whereas Umax exhibited thermal independence for all species. Full article
(This article belongs to the Section Physiology and Biochemistry)
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23 pages, 2914 KB  
Article
Microretention in a River Basin as an Example of Sustainable Stormwater Management—A Case Study
by Maciej K. Bełcik, Aleksandra Mika, Marcin Wdowikowski and Małgorzata Kutyłowska
Sustainability 2026, 18(16), 8492; https://doi.org/10.3390/su18168492 - 19 Aug 2026
Viewed by 176
Abstract
While low-impact development and retention strategies are widely studied in urban and agricultural contexts, a distinct knowledge gap remains regarding the quantitative evaluation of dispersed, natural microretention structures in small, ungauged, mountainous forested catchments under complex topographic conditions. To address this limitation, this [...] Read more.
While low-impact development and retention strategies are widely studied in urban and agricultural contexts, a distinct knowledge gap remains regarding the quantitative evaluation of dispersed, natural microretention structures in small, ungauged, mountainous forested catchments under complex topographic conditions. To address this limitation, this study provides a novel quantitative assessment of how natural bioretention interventions—specifically arcuate deadwood log barriers, cascading reservoir systems, and strategic afforestation—influence runoff reduction and substrate infiltration dynamics. Focusing on the 4.57 km2 basin of the Stankowice Stream in southwestern Poland, the research integrates field geodetic and hydrological measurements with Iszkowski’s empirical flow formulas and high-resolution digital elevation modeling (SCALGO platform). Delineation of 10 key subcatchments revealed that surface runoff potential is heavily concentrated within specific flow pathways rather than determined solely by subbasin area. In unit No. 9, deploying an arcuate arrangement of 19 deadwood logs achieved an 11% reduction in surface runoff (retaining 8662.50 m3), whereas coupling these log structures with a downstream cascading two-dam system significantly enhanced retention performance by establishing 79,065.68 m3 of depression storage and driving 264,066.16 m3 of subsurface infiltration. Furthermore, multi-scenario land use modeling demonstrated that transforming land cover to forest reduced surface runoff by over 70% in topographically steep subcatchments (e.g., unit No. 7). These findings demonstrate that effective flood mitigation in headwater catchments requires a systemic, targeted hybrid strategy combining decentralized bioretention with localized storage nodes, offering a transferable framework for sustainable regional water governance. Full article
(This article belongs to the Section Sustainable Water Management)
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22 pages, 7092 KB  
Article
A Water Budget Evaluation of a Tile-Drain-Fed Irrigation Pond in the Willamette Valley, Oregon, USA
by Noah Goodwin Bain, Carlos G. Ochoa, Derek C. Godwin, Abigail Tomasek and Arshdeep Singh
Hydrology 2026, 13(8), 211; https://doi.org/10.3390/hydrology13080211 - 4 Aug 2026
Viewed by 287
Abstract
Agricultural systems face heightened risks from extreme weather events and water insecurity. Producers commonly use irrigation ponds to secure or improve crop yields. The hydrology and storage efficiency of irrigation ponds in the Willamette Valley, Oregon, USA, are not well understood. This study [...] Read more.
Agricultural systems face heightened risks from extreme weather events and water insecurity. Producers commonly use irrigation ponds to secure or improve crop yields. The hydrology and storage efficiency of irrigation ponds in the Willamette Valley, Oregon, USA, are not well understood. This study evaluated the hydrological interactions of a tile-drain-fed irrigation pond. A water balance approach was applied over the irrigation season using weather data, evaporation estimates, metered irrigation withdrawals, and bathymetry analysis for pond stage–volume estimates to quantify water budget components. Irrigation withdrawals were the largest output, with 75% of effective pond storage utilized, followed by evaporation (24.5%). Evaporation far exceeded precipitation over the same period. The unaccounted-for proportion of the water balance was negligible, indicating that net drain tile inflows and groundwater exchange had a minimal impact on seasonal irrigation water availability and seepage losses. This study provides an example for measuring water balance components and assessing water input–output relationships of an irrigation pond within a headwaters stream and catchment (<5 ha) of an important agricultural corridor in the Pacific Northwest region in the USA. The study methodology can be replicated in other similar agricultural areas with irrigation ponds worldwide. Findings from this study can be used by farmers, irrigation districts, and other stakeholders to better inform irrigation planning and water management decisions for similar site conditions. Full article
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12 pages, 1032 KB  
Article
Microplastic Pollution in Headwater Streams and a Small Freshwater Fish
by Elizabeth S. Gordon, Maeve H. Ahern, Nicole E. Burdick, Kaitlyn E. Prentiss, Paulina Torres and Daniel P. Welsh
Microplastics 2026, 5(3), 144; https://doi.org/10.3390/microplastics5030144 - 18 Jul 2026
Viewed by 1115
Abstract
Microplastic pollution is an increasingly important environmental concern, especially in aquatic ecosystems. However, research on microplastics has historically been skewed towards marine systems and large freshwater systems, leading to an incomplete picture of the potential prevalence and scope of this type of pollution. [...] Read more.
Microplastic pollution is an increasingly important environmental concern, especially in aquatic ecosystems. However, research on microplastics has historically been skewed towards marine systems and large freshwater systems, leading to an incomplete picture of the potential prevalence and scope of this type of pollution. In order to understand the potentially ubiquitous nature of freshwater microplastics, our study focused on 11 small headwater streams in north–central Massachusetts (USA) and a small freshwater fish species, the Blacknose dace (Rhinichthys atratulus). Microplastics were found in all sampled streams. Considerable variation in the number of stream microplastics existed, with concentrations ranging from 0.3 to 45.1 pieces per cubic meter of water. Microplastics were assessed in 14–20 fish from each of five of these streams. The vast majority of all fish had microplastics, with some individuals having over 200 pieces in their digestive tract. The number of fish microplastics was not found to be statistically related to the amount of water microplastics, the length of the fish, or the land use surrounding the streams. Together, these results illustrate that microplastics are prevalent and that small freshwater ecosystems need to be considered when assessing the full impact this source of pollution may have on ecosystems. Full article
(This article belongs to the Special Issue Microplastics in Freshwater Ecosystems)
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14 pages, 36176 KB  
Article
Of Pigs and Pillars: Wild Pigs (Sus scrofa) Impair a Critical Functional Group of Headwater Streams, Threatening an Important Component of the Environmental Pillar of Sustainability
by Carlos D. Camp and Elijah Marshall
Sustainability 2026, 18(14), 7269; https://doi.org/10.3390/su18147269 - 16 Jul 2026
Viewed by 348
Abstract
Comprising the greatest proportion of stream networks, headwater streams provide critical ecosystem services to entire river networks by sustaining water quality and biodiversity as well as controlling floods and groundwater recharge. As such, they are important contributors to the environmental pillar of sustainability. [...] Read more.
Comprising the greatest proportion of stream networks, headwater streams provide critical ecosystem services to entire river networks by sustaining water quality and biodiversity as well as controlling floods and groundwater recharge. As such, they are important contributors to the environmental pillar of sustainability. Unfortunately, they are also among the most vulnerable ecosystems. In Appalachian headwater streams, larval salamanders represent a critical functional group because of their strong influence on ecosystem function. Wild pigs (Sus scrofa) rank among the most notorious invasive species, with widespread, negative impacts on native habitats and biodiversity. We tested the impacts of wallowing by wild pigs on the salamander larval community in Appalachian headwater streams in northeastern Georgia and found a significant drop in both numbers and diversity of larvae in wallows. Because downstream networks and the communities that depend upon them rely on healthy headwater streams, understanding exactly how the impairment of this functional group by wild pigs impacts energy flow, nutrient cycling, carbon sequestration, and water quality requires further investigation. Full article
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16 pages, 3655 KB  
Article
Hierarchical Environmental Filters Structure Benthic Macroinvertebrate Assemblages in Relatively Well-Preserved Mediterranean Mountain Headwater Streams
by Gabriel Rosário, Laís Cristina Gonçalves, Manuel Lopes Lima, João Queirós, Sara Sampaio, Joshua Díaz Caballero, Maria de Jesus Gonzalez, Paulo Célio Alves, Edna Cabecinha, Guilherme Rossi Gorni and Simone Varandas
Water 2026, 18(12), 1448; https://doi.org/10.3390/w18121448 - 12 Jun 2026
Viewed by 441
Abstract
Mountain stream ecosystems are often considered among the least disturbed freshwater environments; however, increasing land-use pressures may affect their ecological integrity even under apparently high-water quality conditions. This study aimed to assess the relative influence of landscape, physicochemical, and hydromorphological factors on benthic [...] Read more.
Mountain stream ecosystems are often considered among the least disturbed freshwater environments; however, increasing land-use pressures may affect their ecological integrity even under apparently high-water quality conditions. This study aimed to assess the relative influence of landscape, physicochemical, and hydromorphological factors on benthic macroinvertebrate communities in three sub-catchments (Ambroz, Jerte, and Tiétar) of the Sierra de Gredos (Central Spain). A total of 33 sampling sites were surveyed, and macroinvertebrate assemblages were analyzed in relation to environmental variables using partial Redundancy Analysis (pRDA) and variance partitioning. All sites were classified as having “Excellent” ecological status based on the Iberian Biological Monitoring Working Party (IBMWP) index. However, multivariate analyses revealed clear spatial patterns and responses to environmental gradients. Results indicated that catchment-scale landscape characteristics defined the pool of potential colonizers, while local physicochemical and hydromorphological conditions acted as secondary filters structuring macroinvertebrate assemblages. Landscape variables explained the largest fraction of variance in community structure (30.6%), followed by physicochemical parameters (29.0%) and hydromorphological indices (24.9%), with a significant shared component (16.5%) indicating interactions among drivers. Agricultural land use, particularly in the Jerte sub-catchment, was associated with shifts in community composition, favoring tolerant taxa such as Diptera, while sub-catchments dominated by natural vegetation supported higher richness of sensitive groups, including Ephemeroptera and Plecoptera. These findings highlight the importance of multi-scale processes in structuring mountain stream communities and reveal limitations of traditional biotic indices in detecting early ecological changes. The results support the integration of catchment-scale variables into ecological assessment frameworks and emphasize the need for preventive, basin-scale management strategies to maintain ecological integrity under increasing anthropogenic pressure. Full article
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28 pages, 50253 KB  
Technical Note
Limitations of a Low-Cost Camera System for Monitoring Streamflow in an Extremely Small Forested Headwater Stream
by Tyler Wong and Steve W. Lyon
Water 2026, 18(11), 1380; https://doi.org/10.3390/w18111380 - 5 Jun 2026
Viewed by 537
Abstract
Headwater stream dynamics are vital for understanding hydrological and ecological processes in watersheds; however, traditional monitoring methods can be costly and time-consuming. This technical note documents the limitations and challenges encountered when deploying a low-cost camera system for continuous streamflow monitoring in a [...] Read more.
Headwater stream dynamics are vital for understanding hydrological and ecological processes in watersheds; however, traditional monitoring methods can be costly and time-consuming. This technical note documents the limitations and challenges encountered when deploying a low-cost camera system for continuous streamflow monitoring in a forested headwater stream in Ohio, USA. The study stream, with a channel width of less than 1 m and watershed of 0.4 km2, is much smaller than previously studied streams. The camera system was constructed using inexpensive and easily accessible electronics, and it enabled application of large-scale particle image velocimetry (LSPIV) to videos collected at a frequency of 15 min. The application of LSPIV to much larger streams is well-established in previous studies; however, its application to extremely small headwater streams is understudied. Preliminary testing in a flume showed that this system was capable of providing accurate discharge measurements. In the field, however, a rating curve calibrated based on the LSPIV-derived flow estimates had an R2 value of 0.70, which was weaker than relationships previously reported in the literature. The rating curve overestimated flows at lower channel stages and underestimated them at higher stages when compared to physical discharge measurements. Examination of the videos collected during field deployment revealed that unsteady flow conditions introduced significant variability in the rating curve analysis. Environmental noise from raindrops, illumination conditions, and leaf litter also caused erroneous flow measurements in the LSPIV results. This technical note presents a critical evaluation of the performance of LSPIV-based camera system in extremely small streams, and practitioners and researchers are advised to follow several best practices, offered as lessons learned from our study, to minimize specific sources of error during implementation. Full article
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26 pages, 6291 KB  
Article
Integrated SWAT+ and MODFLOW Modeling Reveals Spatially Heterogeneous Surface Water–Groundwater Interactions in the Headwater Region of the Upper Blue Nile Basin
by Zena Tessema Terefe, Nigussie Haregeweyn, Mitsuru Tsubo, Ayele Almaw Fenta, Taye Minichil Meshesha, Getnet Taye Bawoke, Ashebir Sewale Belay, Alebachew Tareke Kehali, Samuel Berihun Kassa, Takeshi Abe and Atsushi Tsunekawa
Water 2026, 18(11), 1328; https://doi.org/10.3390/w18111328 - 30 May 2026
Viewed by 822
Abstract
Surface water–groundwater interactions play a critical role in regulating hydrological fluxes and sustaining water availability, yet they remain poorly understood in hydrogeologically complex terrains. This study employed an integrated modeling approach combining SWAT+ and MODFLOW to quantify water balance components, groundwater flow dynamics, [...] Read more.
Surface water–groundwater interactions play a critical role in regulating hydrological fluxes and sustaining water availability, yet they remain poorly understood in hydrogeologically complex terrains. This study employed an integrated modeling approach combining SWAT+ and MODFLOW to quantify water balance components, groundwater flow dynamics, and river–aquifer exchanges in the Chemoga watershed, a representative headwater system of the Upper Blue Nile Basin characterized by strong environmental and geological contrasts. Model results revealed substantial spatial heterogeneity in hydrological partitioning, with annual groundwater recharge ranging from 105 to 711 mm (mean = 296 mm; 24% of annual rainfall). Simulated groundwater flow exhibited a pronounced topographic control, with hydraulic heads declining from highland recharge zones toward deeply incised lowland gorges. River–aquifer interactions showed marked spatial variability, with the Chemoga river predominantly acting as a gaining stream in the highland and nick-point gorge sections (up to 2867 m3 d−1), while transitioning to a losing stream in the midland floodplains and lowland gorge areas, with leakage reaching up to 75.0 m3 d−1. These findings highlight the value of integrated, process-based modeling for resolving complex hydrological interactions, advancing understanding of groundwater flow regimes and supporting sustainable groundwater management in the Ethiopian highlands and other similar regions worldwide. Full article
(This article belongs to the Section Hydrogeology)
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8 pages, 467 KB  
Proceeding Paper
A Low-Cost IoT Sensor for Streamflow Monitoring: A Proof-of-Concept Using Commercial off the Shelf (COTS) Hardware
by Konstantinos Ioannou, Stefanos Stefanidis and Ilias Karmiris
Environ. Earth Sci. Proc. 2026, 40(1), 14; https://doi.org/10.3390/eesp2026040014 - 23 Apr 2026
Viewed by 1026
Abstract
Accurate measurement of streamflow is fundamental for water resources management, ecological conservation, flash flood early warning, and climate change impact studies. This study presents a proof of concept on the usage of Internet of Things (IoT) for automatic streamflow measurements using commercial off-the-shelf [...] Read more.
Accurate measurement of streamflow is fundamental for water resources management, ecological conservation, flash flood early warning, and climate change impact studies. This study presents a proof of concept on the usage of Internet of Things (IoT) for automatic streamflow measurements using commercial off-the-shelf (COTS) hardware. The system is designed, implemented, and experimentally evaluated as a low-cost, solar-powered IoT device tailored to small-order streams and headwater tributaries. At its core is the Hall-effect YF-S201 flow sensor. Although primarily designed for closed-conduit applications, the sensor was tested in a controlled setup where stream water was diverted into a short pipe section, enabling continuous monitoring and calibration. This paper provides details on the design and validation of a low-cost (approximately 24 Euros), solar-powered streamflow measurement system based on a water flow sensor, using wireless communications, and cloud storage based on an ESP32 board, PostgreSQL, and a web interface. The device was tested in a simulated environment. Results indicate the proposed device reliably tracks flow variability, while offering portability, energy autonomy, and cost efficiency, and may serve as a feasible alternative for low-infrastructure, temporary deployments. Full article
(This article belongs to the Proceedings of The 9th International Electronic Conference on Water Sciences)
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12 pages, 3834 KB  
Review
An Assessment of the Mechanistic Basis for the High Endemism and Landscape-Scale Biodiversity in Headwater Streams
by John S. Richardson
Diversity 2026, 18(4), 239; https://doi.org/10.3390/d18040239 - 21 Apr 2026
Viewed by 781
Abstract
Observations suggest that headwater streams have lower species diversity within a site than larger streams, but higher beta diversity, and thus gamma diversity, across a catchment. This pattern of diversity includes taxonomic richness and genetic diversity, as well as a high degree of [...] Read more.
Observations suggest that headwater streams have lower species diversity within a site than larger streams, but higher beta diversity, and thus gamma diversity, across a catchment. This pattern of diversity includes taxonomic richness and genetic diversity, as well as a high degree of endemism. I review several mechanisms that potentially contribute to the overall high diversity of freshwater organisms in headwaters, although these mechanisms are interdependent. These include the high numbers of headwater streams, heterogeneity of habitats and resources, founder effects, colonization dynamics, isolation, and strong selection, all leading to diversification of forms. However, riverscape diversity patterns vary across taxonomic and functional groups, highlighting that patterns of diversity are driven by different processes for different organisms. More explicitly structured sampling designs will better address patterns of taxonomic richness and for a broader range of taxa. It will be interesting to find ways to partition the relative importance of different mechanisms in contributing to the variation in diversity among headwaters. The great importance of headwater streams to global biodiversity conservation is clear, but will be more evident when better assessments of diversity patterns across these small systems are available. Full article
(This article belongs to the Special Issue Aquatic Biodiversity and Habitat Restoration)
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29 pages, 5427 KB  
Article
Integrated Multi-Evidence Modeling of River–Groundwater Interactions and Sustainable Water Use in the Arid Aksu River Basin, Northwest China
by Jingya Ban, Shukun Ni, Zhilin Bao, Bin Wu and Chuanhong Ye
Hydrology 2026, 13(3), 95; https://doi.org/10.3390/hydrology13030095 - 16 Mar 2026
Viewed by 1666
Abstract
The Aksu River Basin, the main headwater of the Tarim River, contributes more than 70% of the main stream’s runoff and is therefore critical in maintaining hydrological stability in this arid river system. In recent decades, rapid oasis expansion and growing agricultural water [...] Read more.
The Aksu River Basin, the main headwater of the Tarim River, contributes more than 70% of the main stream’s runoff and is therefore critical in maintaining hydrological stability in this arid river system. In recent decades, rapid oasis expansion and growing agricultural water withdrawals have intensified competition for surface and groundwater, posing increasing ecological risks to the downstream Tarim River Basin. To quantitatively characterize river–groundwater hydrological responses under intensive water use, we combined statistical analysis, field observations, and distributed hydrological modeling within a basin-scale conceptual framework. Multiple lines of evidence—water level monitoring, hydrochemical tracers, stable isotopes, and the integrated surface–groundwater model MIKE SHE—were used to identify river–groundwater interaction mechanisms in the Aksu alluvial plain. Results reveal a typical three-stage spatial exchange pattern: river recharge to groundwater in the upstream reach, groundwater discharge to the river in the midstream, and renewed river infiltration to groundwater downstream. The patterns inferred from water levels, hydrochemistry, and isotopes are broadly consistent, while water-level data better resolve left–right bank asymmetry. The MIKE SHE model supports the seasonal bidirectional exchange dynamics and reproduces runoff behavior with acceptable performance (RMSE and residual standard deviation within 20% of observed means and R2 > 0.7 during both calibration (2010–2017) and validation (2018–2021)). The proposed multi-evidence framework captures the spatio-temporal variability of river–groundwater interactions in arid regions and provides spatially differentiated guidance for conjunctive surface–groundwater regulation and integrated water resources management in the Tarim River Basin. Full article
(This article belongs to the Section Surface Waters and Groundwaters)
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14 pages, 2397 KB  
Article
Limits in the Recovery of the Headwater Stream Litavka, Czech Republic: A 22-Year Experience
by Kateřina Dočkalová, Pavel Chvojka, Jiří Kopáček, Josef Křeček, Jan Špaček, Marie Uhrová and Evžen Stuchlík
Water 2026, 18(4), 479; https://doi.org/10.3390/w18040479 - 13 Feb 2026
Viewed by 640
Abstract
Despite reductions in sulphur and nitrogen emissions, lakes and streams in Europe and North America have shown only partial recovery from acidification. This study aims to assess the chemical and biological recovery of the upper stretch of the Litavka River, currently on of [...] Read more.
Despite reductions in sulphur and nitrogen emissions, lakes and streams in Europe and North America have shown only partial recovery from acidification. This study aims to assess the chemical and biological recovery of the upper stretch of the Litavka River, currently on of the most acidic stream in the Czech Republic. Water composition and macroinvertebrates were studied for 1999, 2010, and 2021, along with long-term data on hydrology and climate. Over these 22 years, concentrations of SO42−, base cations, conductivity, and toxic Al forms (Ali) significantly decreased, but pH only increased from 4.2 to 4.3. Biological recovery was most evident during 1999–2010, with an increase in the number of taxa and the appearance of less acid-tolerant taxa such as stonefly Diura bicaudata and caddisfly Rhyacophila sp., mainly associated with decreased Ali toxicity. Subsequently, however, despite continued chemical improvement, macroinvertebrate diversity decreased, and sensitive taxa were again absent in 2021. Average annual temperature increased by 2.4 °C over the past 50 years (1970–2020) while precipitation remained unchanged, resulting in significant aridification of the regional climate. We attribute the lack of biological recovery in 2021 to climate-related changes, including more frequent dry periods and floods. Although partial biological recovery of the river followed chemical recovery, the increasing frequency of hydrological extremes has likely become the main limiting factor. Full article
(This article belongs to the Section Ecohydrology)
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24 pages, 6704 KB  
Article
Exploratory Assessment of Short-Term Antecedent Modeled Flow Memory in Shaping Macroinvertebrate Diversity: Integrating Satellite-Derived Precipitation and Rainfall-Runoff Modeling in a Remote Andean Micro-Catchment
by Gonzalo Sotomayor, Raúl F. Vázquez, Marie Anne Eurie Forio, Henrietta Hampel, Bolívar Erazo and Peter L. M. Goethals
Biology 2026, 15(3), 257; https://doi.org/10.3390/biology15030257 - 30 Jan 2026
Viewed by 2001
Abstract
Estimating runoff in ungauged catchments remains a major challenge in hydrology, particularly in remote Andean headwaters where limited accessibility and budgetary constraints hinder the long-term operation of monitoring networks. This study integrates satellite-derived rainfall data, hydrological modeling, and benthic macroinvertebrate diversity analysis to [...] Read more.
Estimating runoff in ungauged catchments remains a major challenge in hydrology, particularly in remote Andean headwaters where limited accessibility and budgetary constraints hinder the long-term operation of monitoring networks. This study integrates satellite-derived rainfall data, hydrological modeling, and benthic macroinvertebrate diversity analysis to explore how short-term antecedent flow conditions relate to temporal variation in community structure. The research was conducted in a pristine 0.26 km2 micro-catchment of the upper Collay basin (southern Ecuador). Daily simulated discharge was used to compute antecedent flow descriptors representing short-term variability and cumulative changes in stream conditions, which were related to taxonomic (i.e., H = Shannon diversity, E = Pielou evenness, and D = Simpson dominance) and functional indices (i.e., Rao = Rao’s quadratic entropy, FAD1 = Functional Attribute Diversity, and wFDc = weighted functional dendrogram-based diversity) using Generalized Additive Models. Results showed progressively higher hydrology–biology associations with increasing antecedent flow integration length, suggesting that biological variability responds more strongly to cumulative than to instantaneous flow conditions. Among hydrological descriptors, the cumulative magnitude of negative flow changes was consistently associated with taxonomic diversity. H and E showed more coherent and robust patterns than functional metrics, indicating a faster response of community composition to short-term hydrological variability, whereas functional diversity integrates slower ecological processes. While based on modeled discharge under severe hydrometeorological data limitations, this study provides a practical ecohydrological starting point for identifying short-term hydrological memory signals potentially relevant to aquatic biodiversity in ungauged headwater systems. Full article
(This article belongs to the Section Marine and Freshwater Biology)
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25 pages, 7572 KB  
Article
Streambed Microbial Activity and Its Spatial Distribution in Two Intermittent Stream Networks
by Andrielle L. Kemajou Tchamba, Charles T. Bond, Brett A. Nave, Claire Utzman, Jerald Ibal, Delaney M. Peterson, C. Nathan Jones, Carla L. Atkinson, Erin C. Seybold, Robert J. Ramos, Amy J. Burgin, Lydia H. Zeglin, Yaqi You, Ken Aho, Kevin A. Kuehn and Colin R. Jackson
Microorganisms 2026, 14(1), 71; https://doi.org/10.3390/microorganisms14010071 - 29 Dec 2025
Viewed by 1135
Abstract
Headwater streams comprise almost 90% of global river networks, and their microorganisms play critical roles in organic matter decomposition and nutrient cycling. These functions, however, are affected by recurrent drying and rewetting. This study examined spatial variation in microbial enzyme activity tied to [...] Read more.
Headwater streams comprise almost 90% of global river networks, and their microorganisms play critical roles in organic matter decomposition and nutrient cycling. These functions, however, are affected by recurrent drying and rewetting. This study examined spatial variation in microbial enzyme activity tied to organic carbon degradation (β-glucosidase, phenol oxidase, and peroxidase) and nitrogen (N-acetylglucosaminidase) and phosphorus (phosphatase) mineralization in water, epilithic biofilm, leaf litter, and sediment in two intermittent streams: Gibson Jack Creek (Idaho, USA) and Pendergrass Creek (Alabama, USA), representing different climactic and physiographic settings. Microbial activity was greater in Gibson Jack Creek, where the activity of leaf litter enzymes varied along the stream network, and there were strong correlations in microbial activity between different stream habitats. Microbial activity in Pendergrass Creek showed primarily within-habitat associations. Activity in water, sediment, and biofilm showed broader spatial heterogeneity in both stream networks. Ratios of microbial activity (enzyme stoichiometry) suggested that microbial communities in both systems were primarily limited by carbon and phosphorus, although there was more spatial variation in nitrogen limitation, particularly in water and sediment at Pendergrass Creek and in biofilm at Gibson Jack Creek. These findings underscore the spatial heterogeneity and environmental sensitivity of microbial processes in intermittent streams. Full article
(This article belongs to the Section Environmental Microbiology)
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19 pages, 3045 KB  
Article
Mountains, Lakes, and Ancient Drainage Networks Sculpt the Phylogeographic Architecture of the Stream Headwater Fish Acrossocheilus kreyenbergii in China
by Yun Chen, Guangmin Deng, Ziyu Le and Cuizhang Fu
Genes 2025, 16(12), 1393; https://doi.org/10.3390/genes16121393 - 21 Nov 2025
Cited by 1 | Viewed by 1430
Abstract
Background: Phylogeographic surveys of obligate freshwater fishes could serve as a pivotal lens through which the biological footprints of historic drainage rearrangements can be deciphered. Methods: Focusing on the headwater-restricted cyprinid Acrossocheilus kreyenbergii in the Pearl, Yangtze, and Huai river basins, we examined [...] Read more.
Background: Phylogeographic surveys of obligate freshwater fishes could serve as a pivotal lens through which the biological footprints of historic drainage rearrangements can be deciphered. Methods: Focusing on the headwater-restricted cyprinid Acrossocheilus kreyenbergii in the Pearl, Yangtze, and Huai river basins, we examined variations in mitochondrial cytochrome b gene (Cyt b) to elucidate the phylogeographic architecture and evolutionary history of this stream fish in South–Central China through integrative analyses of phylogeny, ancestral area reconstruction, genetic structure, and population demography. Results: A time-calibrated phylogeny recovered two primary lineages, K-I and K-II, which diverged ca. 2.15 Ma: K-I split into K-Ia (Huai River) and K-Ib (Yangtze–Poyang Lake catchment) at 1.53 Ma, whereas K-II gave rise to K-IIa, K-IIb, and K-IIc through sequential divergences at 1.29 Ma and 0.83 Ma, with K-IIa restricted to the Poyang Lake catchment. K-IIb was shared between the Poyang Lake catchment and the Qiupu River (Yangtze basin), and K-IIc was distributed in the Xijiang River (Pearl basin) as well as the Yangtze–Dongting Lake catchment. Conclusions: Our findings reveal that the phylogeographic architecture of A. kreyenbergii was sculpted by a succession of geologic and anthropocentric events: the Late-Cenozoic collapse of the Zhe–Min Uplift first fractured its range; the intervening Mufu–Lianyun–Luoxiao Mountains then acted as a persistent barrier; the large waters of Poyang and Dongting Lakes served as biological filters; and the 2200-year-old Lingqu Canal—constructed during the Qin dynasty—briefly re-established a corridor for gene flow. Together, these forces disrupted and reorganized the species’ genetic connections, leaving a visible imprint today. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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