Search Results (9,321)

Background/Objectives: Glyphosate-based herbicides (GBHs) are widely used agrochemicals implicated in nephrotoxicity through mechanisms involving oxidative stress, inflammation, and tissue remodeling. Natural peptides such as melittin possess potent anti-inflammatory and antioxidant properties; however, their therapeutic use is limited by instability and toxicity. Nanotechnology-based encapsulation presents a promising approach to overcoming these challenges. Objective: This study aimed to evaluate the protective effects of melittin-loaded chitosan–TPP nanoparticles (MEL-NPs) against glyphosate-induced nephrotoxicity in rats, with emphasis on oxidative, inflammatory, and apoptotic pathways. Methods: Female Wistar rats were divided into four groups: control, glyphosate (5 mg/kg/day, 25 days), glyphosate + free melittin, and glyphosate + MEL-NPs (40 µg/kg, orally, 3 times/week). Renal function biomarkers, oxidative stress parameters (MDA, GSH, SOD, CAT, NO), cytokines (TNF-α, IL-6), and serum protein/iron indices were assessed. Western blotting (Nrf2, NGAL), histopathology (H&E), and immunohistochemistry (Bax) were performed. Nanoparticles were characterized by TEM, FTIR, and UV–Vis spectroscopy. Results: Glyphosate exposure caused renal dysfunction, including elevated plasma urea and creatinine levels, and reduced creatinine clearance, indicating impaired glomerular filtration efficiency, oxidative stress (↑increased MDA, NO; ↓decreased GSH, SOD), and upregulation of pro-inflammatory cytokines. Histology revealed tubular degeneration and inflammatory infiltration, while NGAL and Bax were strongly induced. Nrf2 expression was elevated as a compensatory response. Free melittin partially ameliorated these alterations, whereas MEL-NPs provided superior protection, restoring renal function, normalizing oxidative balance, reducing NGAL and Bax expression, and preserving renal histoarchitecture. Conclusions: Melittin nanoparticles confer robust renoprotection against glyphosate-induced nephrotoxicity in rats by modulating oxidative stress, suppressing inflammation, and regulating Nrf2/Bax signaling. These findings highlight nano-melittin as a promising therapeutic platform for managing herbicide-related renal disorders. Full article

In vitro fertilization (IVF) exposes embryos to environmental stressors that can disrupt early development and confer long-term health risks, though the mechanisms remain poorly understood. Here, we tested the hypothesis that reducing incubation temperature during the first zygotic cleavage would promote long-term developmental stability in IVF-conceived offspring. Using a mouse model, we compared the long-term effects of standard (37 °C) versus reduced (35 °C) IVF culture temperature on energy balance, circadian rhythms, sleep architecture, and brain histone modifications. Although offspring from both IVF groups exhibited increased body mass without notable effects on glucose metabolism, significant disruptions in circadian rhythms and sleep–wake patterns were detected. The 37 °C group exhibited altered amplitudes in oxygen consumption rhythms and respiratory exchange ratios, as well as pronounced alterations in sleep–wake patterns, including reduced sleep duration and increased nighttime activity. The 35 °C group displayed intermediate phenotypes, substantiating the importance of optimizing embryo incubation parameters. These metabolic and behavioral changes were paralleled by altered histone modifications in the cerebral cortex of IVF offspring, suggesting an epigenetic basis for circadian misalignment. Our results identify disrupted circadian rhythm and sleep architecture as a novel mechanism contributing to metabolic dysfunction in IVF-conceived offspring. The partial mitigation of these effects through reduced culture temperature underscores the importance of optimizing IVF protocols to minimize long-term epigenetic and metabolic risks. Full article

Feed and water scarcity are major challenges for the sustainability of livestock production, particularly in semi-arid regions with structural limitations in resource availability. In this context, the valorization of agro-industrial by-products contributes to circular agriculture, reduces waste, and promotes more efficient resource use, in line with the United Nations Sustainable Development Goals. This study evaluated the inclusion of partial mixed tomato residue (PMR) silage in sheep diets and its effects on productive performance, total water intake, and meat quality. Eighteen ewe lambs were assigned to two groups: control (concentrate and deferred pasture) and PMR (tomato residue silage and deferred pasture). The PMR silage had a pH of 3.97 and was mainly characterized by lactic and acetic acids, with minor amounts of propionic and butyric acids. The butyric acid concentration (8.9 g kg⁻¹ DM) slightly exceeded the recommended threshold (0.5% DM), suggesting some clostridial activity but remaining below levels associated with severe deterioration. Animals fed PMR silage showed a 36% higher dry matter intake (p = 0.001), with greater intake of total digestible nutrients and fiber. This translated into a 54% higher average daily gain (p = 0.02) and an 11% greater final body weight compared with the control group (p = 0.02). Dietary water intake was also higher in the PMR group, reducing direct water consumption from drinkers by 38% (p < 0.001). Meat quality parameters were unaffected by the diet. Pesticide residue screening by LC-MS/MS revealed no detectable levels of abamectin, cymoxanil, chlorothalonil, difenoconazole, or mancozeb in silage. In meat samples, only chlorothalonil was tested and it was not detected. However, the use of PMR silage increased direct energy demand due to transport and compaction, while feeding costs per unit of weight gain were reduced. Overall, PMR silage proved to be a safe, fermentatively stable, and effective feeding alternative that enhances performance, reduces direct water intake, and maintains meat quality, representing a viable strategy for small ruminant production in water-limited regions. Full article

Calcium disorders remain a major challenge during the transition period of dairy cows, and oral calcium supplements are widely used to prevent postpartum hypocalcemia. This study evaluated the effects of administering an oral Ca-bolus containing calcium sulfate, acetate, and formate on postpartum mineral status and energy balance in multiparous Simmental cows. Twenty cows (mean parity 3.5 ± 0.51) were fed a prepartum diet with a negative dietary cation–anion difference (DCAD) and enrolled at calving if body condition score was between 3.0 and 3.5 and urine pH ranged from 6.2 to 6.8 during the wk before parturition. Animals were blocked by parity and randomly assigned to a control group (n = 10) or to a treatment group (n = 10) receiving two oral boluses (each 175 g; 45.14 g calcium plus 40,250 IU vitamin D₃), administered immediately after calving and 24 h later. Blood samples were collected up to 48 h postpartum for calcium, phosphorus, and magnesium analyses, and up to 14 d postpartum for glucose and β-hydroxybutyrate. Both groups were normocalcemic at calving, and no significant treatment effects were detected for any parameter at any time point. However, a short-lived numerical increase in calcium was observed 6 h after bolus administration. These results suggest that additional oral Ca supplementation may not be required in well-managed Simmental cows receiving a prepartum negative DCAD diet. Full article

Obesity is a major global health issue linked to metabolic disorders, chronic inflammation, and systemic complications, with high-fat diets (HFDs) playing a key role by disrupting intestinal balance and promoting oxidative stress. This study investigates Protaetia brevitarsis-derived protein hydrolysate (PBPH), an insect-derived bioactive peptide extract, as a potential intervention to counteract HFD-induced metabolic disturbances. Female ICR mice were divided into three groups: control diet, HFD, and HFD + PBPH, with PBPH (obtained by alcalase hydrolysis and ultrafiltration) administered daily for eight weeks. Researchers assessed adipokine levels, inflammatory cytokines, antioxidant enzymes, and apoptotic markers using qPCR, ELISA, histology, and immunohistochemistry. PBPH supplementation significantly improved metabolic parameters by lowering leptin, adipsin, resistin, IL-1β, TNF-α, and IL-6, while restoring antioxidant balance and reducing pro-apoptotic signals. Histological analyses confirmed preserved intestinal tissue and reduced inflammation. Overall, this study highlights PBPH’s promising therapeutic role in addressing obesity-related metabolic dysfunctions through its multifaceted effects on inflammation, oxidative stress, and apoptosis. It underscores the potential of insect-derived peptides as sustainable, innovative dietary interventions for improving metabolic health. Full article

Aim: This current research aims to determine the predictive value of the ratio of triglyceride (TG)/high-density lipoprotein cholesterol (HDL-C), index of triglyceride–glucose (TyG), homeostatic model assessment for insulin resistance (HOMA-IR) score, and anthropometric measurements at the onset of non-alcoholic fatty liver disease (NAFLD) in obese kids and juveniles. It also sought to assess how novel cardiovascular risk markers are affected in obese pediatric patients with NAFLD. Materials and Methods: Between November 2024 and May 2025, a total of 199 pediatric patients were prospectively evaluated, including 150 children with obesity and 49 entirely healthy controls. Two categories of obese patients were created based on whether or not they had non-alcoholic fatty liver disease. These groups were compared with each other and with the control group in terms of HOMA-IR score, index of TyG, ratio of TG/HDL-C, anthropometric parameters (percentage of body fat [BFP], index of body mass [BMI], body fat mass [BFM], waist circumference [WC]), and cardiovascular risk markers. The cutoff values, sensitivity, and specificity of the HOMA-IR score, ratio of TG/HDL-C, anthropometric measurements, and index of TyG in predicting NAFLD were assessed using Receiver Operating Characteristic (ROC) analysis. Results: Obese kids and juveniles with NAFLD had significantly higher TG/HDL-C ratios, TyG indices, HOMA-IR scores, anthropometric measurements, and cardiovascular risk markers than those without NAFLD (p < 0.001). The TG/HDL-C ratio (AUC: 0.936; 81.8% sensitivity, 95.9% specificity) and the TyG index (AUC: 0.912; 81.8% sensitivity, 91.8% specificity) showed strong predictive value for NAFLD, while HOMA-IR and WC were found to be relatively weaker predictors. Conclusions: The index of TyG and ratio of TG/HDL-C are highly effective parameters in predicting NAFLD development in obese kids and juveniles. Those with increased WC and BFP should be closely monitored for NAFLD development. Pediatric patients with NAFLD should be carefully followed up for potential cardiovascular diseases. Full article

Corn (Zea mays L.) ranks among the most important cereal crops globally, extensively cultivated for food, animal feed, and industrial applications. Its large-scale production generates substantial amounts of agricultural residues such as cobs, husks, stalks, leaves and other, which are often underutilized, leading to environmental concerns. Due to their high carbon content, lignocellulosic structure, and abundant availability, these residues represent a sustainable and low-cost raw material for the synthesis of activated carbon. Corn waste-derived activated carbon has emerged as a promising material for the efficient removal of heavy metals from aqueous solutions. Its high surface area, well-developed porosity, and adjustable surface chemistry, referring to the functional groups on the adsorbent surface that can be modified to enhance affinity toward metal ions, facilitate effective adsorption. This review provides a comprehensive overview of (1) the potential of corn waste biomass as a precursor for activated carbon production, (2) methods of carbonization and activation that influence the textural and chemical properties of the resulting adsorbents, (3) adsorption performance for heavy metal removal under varying experimental parameters such as pH, initial concentration, contact time, and adsorbent dosage, (4) adsorption mechanisms responsible for heavy metal uptake. Reported maximum adsorption capacities vary for different metals, ranging from 2.814–206 mg/g for lead, 0.21–87.72 mg/g for cadmium, 9.6246–175.44 mg/g for chromium, and 0.724–643.92 mg/g for copper. Utilizing corn waste not only provides an eco-friendly approach for managing agricultural residues but also supports the development of efficient adsorbents. Nevertheless, challenges such as scaling up production and evaluating adsorbent performance in real wastewater samples remain and require further investigation. Finally, the review highlights key challenges and knowledge gaps in current research and offers recommendations for future studies aimed at advancing the practical application of corn waste–based activated carbons in water treatment. Full article

The miscible process of virgin and aged asphalt in rejuvenated asphalt was studied by molecular dynamics (MD) simulation. In this paper, we used MD software to establish a molecular model of asphalt, and the model of aged asphalt was established by adding ketone and sulfoxide functional groups to the original asphalt. Wood tar rejuvenator (WTR) was selected for rejuvenation of aged asphalt, and parameters such as density, surface free energy, cohesion energy density, and Young’s modulus were used to verify the molecular model. The density, relative concentration, interaction energy, mean square displacement of molecules, diffusion coefficient, mixing free energy, and radial distribution function were used to analyze the action mechanism of the rejuvenator in the rejuvenation process and the suitable service temperature and optimal amount of WTR. The results demonstrated that the WTR with 373 K and 15% mass ratio has the best rejuvenation effect on aged asphalt. The addition of WTR can increase the interaction energy between original and aged asphalt by 12.9%, reduce the Van der Waals potential energy of aged asphalt by 13.85%, and thus ensure the uniform distribution of internal molecules in rejuvenated asphalt. A 15 wt% WTR can reduce the intermolecular distance of asphaltenes from 9.4 Å to 5.2 Å, thereby alleviating the displacement effect during the asphalt aging process. The diffusion coefficients of WT-rejuvenated asphalt at 298 K and 373 K are 28.6% and 44.6% higher than those of extracted oil-rejuvenated asphalt, respectively; thus, WT-rejuvenated asphalt has better crack resistance. Full article

Background/Objectives: This study aimed to determine the effects of weight gain and fatigue on nursing students performing high-fidelity cardiopulmonary resuscitation (CPR) simulation. Methods: A simulation-based research study (with or without a vest) was performed at the Faculty of Health Sciences, University of Castilla la Mancha (Spain), with volunteer nursing students. Vital signs, pain scale, pupillary sizes, analytical parameters, and lifestyle habits were evaluated before, during, and after CPR. The differences between groups were assessed by ANOVA for time and group factors. Results: A total of 31 participants met the inclusion criteria, 15 subjects without a vest and 16 subjects with a vest. The ANOVA results revealed that all the variables presented statistically significant time effects, except for glucose. For the group effect, heart rate, lactate, and cortisol presented statistically significant differences between subjects with and without vests. Conclusions: Vital signs and physiological variables increased during CPR with vests. This could negatively impact the CPR procedure. The implementation of physical training programs aimed at improving the performance of future health professionals during CPR should be studied. Full article

Carotid artery intima–media thickness (cIMT), a pre-clinical vascular change that accompanies atherosclerosis is considered as a cardiovascular risk factor. Coagulation factor XIII (FXIII) stabilizes the fibrin clot and increases its resistance to fibrinolysis. Regarding FXIII Val34Leu polymorphism, the protective effect of the Leu34 allele in the presence of elevated fibrinogen levels against myocardial infarction was demonstrated. Our aim was to investigate the effect of FXIII polymorphisms on cIMT. Patients with obesity (n = 69), type 2 diabetes mellitus (T2DM) (n = 104), and age- and sex-matched healthy controls (n = 82) were enrolled. FXIII polymorphisms (Val34Leu, His95Arg, Intron-K C>G) were determined by RT-PCR with FRET detection and melting curve analysis. cIMT was determined by B-mode ultrasound. Differences in cIMT between control (median: 0.5965, IQR: 0.5115–0.6580 mm) and T2DM (median: 0.7105, IQR: 0.5948–0.7568 mm), as well as between obese (median: 0.6105, IQR: 0.5455–0.6780 mm) and diabetic groups, were found (p < 0.0001 and p = 0.003, respectively). Genotype and allele frequencies of the studied polymorphisms did not differ between subgroups. In the study group (n = 255) after adjustment for age and sex, the presence of Intron-K G allele showed a significant and independent protective effect against cIMT progression in a separate model (p = 0.005) and after adjusting for other parameters associated with cIMT (p = 0.015). FXIII Intron-K G allele provides a protective effect against subclinical vascular disease in the studied population, and this effect is independent of the presence of obesity, as well as T2DM, Leu34 allele, and fibrinogen levels. Full article

Background/Objectives: Occlusal trauma does not initiate periodontitis but may accelerate its progression when inflammation is present. Chlorhexidine (CHX) mouthwash is widely used as an adjunct to periodontal therapy, although its effectiveness in patients with occlusal trauma is insufficiently documented. This study aimed to evaluate the effect of CHX mouthwash on periodontal status in patients with deflective occlusal contacts, in the absence of occlusal adjustments. Materials and Methods: This observational prospective study analyzed data from 52 patients (20 males, 32 females; mean age 41.35 years). Periodontal status was assessed using the Community Periodontal Index of Treatment Needs (CPITN) at baseline, 3 months, and 6 months. Patients were divided into groups based on CHX use and concentration. Statistical analysis evaluated intra- and intergroup variations. Results: Patients using CHX demonstrated statistically significant improvements in CPITN scores at 3 months for all sextants except sextant 5 (p < 0.05). Between 3 and 6 months, further significant improvement was observed only for sextant 6 and for the overall score. In contrast, patients without CHX showed a slight trend toward worsening CPITN values, with no statistically significant differences over the same period. Conclusions: CHX mouthwash significantly improved periodontal parameters in patients with occlusal trauma during the first three months of use. However, improvements plateaued after this period, highlighting the short-term benefits and limitations of CHX. These findings support the adjunctive role of CHX in managing periodontal disease associated with occlusal trauma but reinforce the necessity of mechanical occlusal correction for long-term stability. The CPITN index provides moderate clinical utility compared with full-mouth clinical periodontal measurements. Full article

Educational strategies based on technological models that integrate the dimensions of prevention, screening, and treatment of diabetic foot ulcers are emerging as promising methods to improve nurses’ knowledge, skills, and clinical competencies in primary care. In this investigation, we evaluated the effectiveness of a digital education program, mediated by a virtual learning environment, in enhancing nurses’ clinical knowledge about diabetic foot ulcers. This quasi-experimental intervention study was conducted with 114 nurses, selected for convenience, from the five health districts that make up primary care in the municipality of Teresina, Brazil. Two stages, separated by the educational intervention, allowed us to measure their knowledge levels before and after the implementation of the digital technology. A characterization form and the Nurse Knowledge Assessment Questionnaire on Diabetic Foot were used to evaluate the outcomes. The McNemar test compared the pre- and post-intervention knowledge levels, while accuracy rate-based parameters allowed for the classification of results into performance categories. The intervention effect size was estimated using Cohen’s d test. Results showed substantial improvements in knowledge, particularly in domains related to definition (p = 0.002), risk factors (p < 0.001), associated complications (p < 0.001), signs and symptoms of neuropathies (p < 0.001), application of tests to assess protective sensation (p < 0.001) and foot biomechanics (p < 0.001), risk classification (p < 0.001), and prevention strategies (p < 0.001), with performance ratings predominantly “good” or “excellent” after the intervention. The effect size for paired samples was large (Cohen’s dz = 1.82), based on the total knowledge scores. Findings support the effectiveness signal of the virtual learning environment for knowledge improvement; however, without a control group, we cannot rule out testing effects. Controlled or stepped-wedge trials should confirm causality. Full article

The cooling system is a core component for a vehicle’s powertrains to operate smoothly and maintain a satisfying noise, vibration, and harshness (NVH) performance. However, advances in new energy vehicles bring with them complex requirements for the cooling fan design due to new issues such as increased heat load, dynamic variations, and high-speed vibrations, which demand the optimization of fan dynamics over a wide range of parameters. In this paper, by thoroughly checking the effect of rigid–flexible coupling and the geometrically complex elastic frame of the fan, we propose a combined modeling approach to reduce the computational time of broad-range parameter variation analysis and examine the vibration problem in the cooling fans under various external excitations. First, the complicated frame of the fan is simplified through virtual prototyping based on an experiment. Then, modal transition is applied, reducing the complex kinetic expression, and a time-invariant system model is derived with multi-blade coordinate transformation. Stability and bifurcation analysis are performed regarding different excitation couplings from the rotor, powertrain, and road. The results of the simulation and experiment illustrate that the proposed methodology achieves a substantial reduction in computational time, and all degrees of freedom (DOFs) are divided into two groups including symmetrical and asymmetrical types. The results also imply the great potential for the optimization and control of the high-speed fan’s vibration for new energy cars. Full article

Background/Objectives: Oral candidiasis is frequently encountered in pediatric populations, particularly in infants and toddlers, where the development of immunity and inconsistent oral hygiene contribute to disease susceptibility. While topical antifungal agents remain the standard of care, treatment challenges persist, especially regarding adherence and recurrence. Aloe vera, recognized for its antimicrobial, anti-inflammatory, and mucosal healing properties, may offer therapeutic benefits when used in conjunction with standard regimens. This study aimed to evaluate the adjunctive effect of topical Aloe vera gel, when added to standard antifungal therapy, on reducing fungal load and improving treatment adherence in children with moderate oral candidiasis. Methods: A prospective controlled study was conducted among 54 children diagnosed with moderate oral candidiasis. Participants were randomly assigned to receive either standard topical nystatin or nystatin in conjunction with Aloe vera gel over a 7 day treatment duration. Fungal load was assessed using colony-forming units (CFU) counts from oral swabs collected at baseline and day 7, analyzed via ANCOVA. Additional parameters included treatment adherence, compared using an independent t-test, and clinical recurrence at a 14-day post-treatment follow-up, assessed through logistic regression. Results: Baseline characteristics were similar across groups. By day 7, children in the Aloe vera group exhibited a greater reduction in fungal load compared to those receiving standard therapy alone. Adherence was significantly higher in the aloe group (92.73% vs. 89.21%; p < 0.0001). Regression analysis identified both baseline fungal burden and adherence as factors associated with an increased risk of recurrence. Conclusions: The addition of Aloe vera gel to standard therapy may support a more effective fungal clearance and improved treatment adherence in children with moderate oral candidiasis, suggesting its potential as a complementary treatment option. Given the single-center design, short follow-up, and underpowered recurrence analysis, these findings should be considered preliminary, pending confirmation in larger studies with symptom-anchored endpoints. Full article

The increasing demand for sustainable and cost-effective animal feed alternatives has stimulated the use of agro-industrial by-products in poultry diets. This study evaluated the effects of tambaqui (Colossoma macropomum) residual oil (TRO), derived from aquaculture waste, on productive performance, physiological responses, and egg quality in commercial laying hens. A total of 144 Hisex Brown hens were assigned to diets containing 0%, 1.5%, 3.0%, or 4.5% TRO for 63 days. While TRO inclusion did not affect overall productivity, moderate levels (1.5% and 3.0%) improved egg weight, yolk pigmentation, and internal quality (Haugh unit). Hematological and biochemical parameters indicated metabolic adaptations, with increased cholesterol and decreased triglycerides in treated groups. The yolk fatty acid profile revealed higher omega-3 content with TRO inclusion, but lipid oxidation (TBARS) also increased, especially at higher levels. Sensory evaluation showed reduced aroma and flavor acceptability in eggs from hens fed 3.0% and 4.5% TRO. These findings suggest that moderate TRO inclusion can enhance egg nutritional value and support sustainable aquaculture waste reuse, though excessive levels may compromise product acceptability. Optimal inclusion levels should be further explored to balance metabolic benefits, oxidative stability, and consumer preferences. Full article