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17 pages, 2079 KB  
Article
Comparative Analysis of Statistical and AI-Based Methods for Livestock Monitoring in Extensive Systems
by Marco Bonfanti, Dominga Mancuso, Giulia Castagnolo and Simona Maria Carmela Porto
Appl. Sci. 2025, 15(20), 11116; https://doi.org/10.3390/app152011116 - 16 Oct 2025
Viewed by 255
Abstract
In recent years, the research focusing on extensive farming systems has attracted considerable interest among experts in the field. Environmental sustainability and animal welfare are emerging as key elements, assuming a crucial role in global agriculture. In this context, monitoring animals is important [...] Read more.
In recent years, the research focusing on extensive farming systems has attracted considerable interest among experts in the field. Environmental sustainability and animal welfare are emerging as key elements, assuming a crucial role in global agriculture. In this context, monitoring animals is important not only to ensure their welfare, but also to preserve the balance of the land. Inadequate grazing management can in fact damage vegetation due to soil erosion. Therefore, monitoring the habits of animals during grazing is a challenging and crucial task for livestock management. Internet of Things (IoT) technologies, which allow for remote and real-time monitoring, may be a valid solution to these challenges in extensive farms where farmer-to-animal contact is not usual. In this regard, this paper examined three different methods to classify the behavioral activities of grazing cows, by using data collected with collars equipped with accelerometers. Three distinct approaches were compared: the former based on statistical methods, and the other on the use of Machine and Deep Learning techniques. From the comparison of the results obtained, strengths and weaknesses of each approach were examined, so to determine the most appropriate choice in relation to the characteristics of extensive livestock systems. In detail, Machine and Deep Learning-based approaches were found to be more accurate but highly energy-intensive. Therefore, in rural environments, the approach based on statistical methods, combined with LPWAN applications, was preferable due to its long range and low energy consumption. Ultimately, the statistical approach was found to be 64% accurate in classifying four behavioral classes. Full article
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14 pages, 1620 KB  
Article
Cattle Preference in Warm-Season Grasses: Effects of Seasonal Growth, Leaf Morphology, and Leaf Anatomy
by Florencia Marcón, Elio L. Di Lorenzo, Myriam C. Peichoto and Carlos A. Acuña
Grasses 2025, 4(4), 40; https://doi.org/10.3390/grasses4040040 - 9 Oct 2025
Viewed by 208
Abstract
Warm-season grasses are the main source of feed in tropical and subtropical beef cattle production systems. The objective was to assess cattle preference among three warm-season grasses and explore its relationship with forage yield and plant structural traits. The three species were cultivated [...] Read more.
Warm-season grasses are the main source of feed in tropical and subtropical beef cattle production systems. The objective was to assess cattle preference among three warm-season grasses and explore its relationship with forage yield and plant structural traits. The three species were cultivated in 2 × 2 m plots using a completely randomized design. Cattle preference was evaluated in spring (December 2016 and 2017), summer (March 2017), and autumn (May 2017) using six Braford steers that grazed the plots for 4 h on two consecutive days. Pre-grazing forage yield, plant height, leaf-blade length, leaf-blade width, and the proportions of five leaf tissues at three leaf regions were measured at each date. Cattle preference was variable among the three species and evaluation dates. Paspalum atratum exhibited the highest pre-grazing forage yield, and constituted the tallest plants with the longest leaves during the summer. Urochloa brizantha showed the greatest proportion of vascular bundle sheath (17–30% at the midrib region, 25–31% at the interveinal region and 14–23% at the margin region) and P. atratum exhibited the greatest number of primary vascular bundle. Cattle preference was negatively correlated with the number of primary vascular bundle, pre-grazing forage yield, plant height and leaf-blade length. Full article
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29 pages, 3006 KB  
Review
Systematic Literature Review on Donkeys (Equus asinus): Husbandry and Welfare in Europe
by Naod Thomas Masebo, Beatrice Benedetti, Maria Gaia Angeloni, Leonie Lee, Daniele Bigi and Barbara Padalino
Animals 2025, 15(19), 2768; https://doi.org/10.3390/ani15192768 - 23 Sep 2025
Viewed by 1170
Abstract
The number of donkeys in Europe has significantly declined in recent decades due to mechanization; however, recently, the demand for donkey milk and other purposes has led to a slight increase in their population. However, information on how they are kept and managed, [...] Read more.
The number of donkeys in Europe has significantly declined in recent decades due to mechanization; however, recently, the demand for donkey milk and other purposes has led to a slight increase in their population. However, information on how they are kept and managed, and their welfare is limited. This review aimed to explore the husbandry, management, and welfare of donkeys (Equus asinus) across European Union member states, Switzerland, and the United Kingdom. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) technique was used. The search was conducted using Scopus and Web of Science, identifying 797 records that were screened using titles, keywords, and abstracts, resulting in 78 retained records. An additional 19 records were identified using snowballing and experts’ suggestions, bringing the total to 97. Dairy donkeys have been studied mainly in Italy, and there they are usually managed under extensive to semi-intensive husbandry systems. Donkeys involved in human intervention therapies are generally managed semi-intensively. Based on the literature, most donkeys are provided with shelter and outdoor access, and this can be with or without pasture, except the free-range donkeys that graze year-round. Health and management-related issues (e.g., obesity, dental disorders, and hoof disorders) could be overlooked, potentially compromising their welfare. The feeding management of donkeys is generally traditional and poorly studied, relying mainly on forages supplemented with concentrates. Most donkeys suffer from overweight/obesity except for lactating donkeys, which are often underweight. This may indicate unbalanced feeding practices. Improved understanding of housing and feeding management is essential for establishing evidence-based welfare guidelines tailored to the donkeys’ species-specific needs. Full article
(This article belongs to the Special Issue Working Equids: Welfare, Health and Behavior)
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14 pages, 9504 KB  
Article
Evaluating Habitat Conditions for the Ringlet Butterfly (Erebia pronoe glottis) in a Multi-Use Mountain Landscape in the French Pyrenees
by Martin Wendt and Thomas Schmitt
Diversity 2025, 17(8), 554; https://doi.org/10.3390/d17080554 - 5 Aug 2025
Viewed by 1395
Abstract
We conducted a mark–release–recapture study of the ringlet butterfly, Erebia pronoe glottis, in the Pyrenees to study population density, flight activity, dispersal, and nectar plant preferences. We found differences between both sexes in population density (males: 48/ha; females: 23/ha), sex ratio (2.1), [...] Read more.
We conducted a mark–release–recapture study of the ringlet butterfly, Erebia pronoe glottis, in the Pyrenees to study population density, flight activity, dispersal, and nectar plant preferences. We found differences between both sexes in population density (males: 48/ha; females: 23/ha), sex ratio (2.1), and behaviour (75.4 vs. 20.5% flying). Both sexes used a wide range of nectar plants (Asteraceae, 40.6%; Apiaceae, 34.4%; Caprifoliaceae, 18.8%). However, local abundance appeared to be limited by the availability of nectar plants. Compared to a population of an extensively used pasture in the Alps, a significant increase in flight activity, but not in range, was observed. Movement patterns showed the establishment of home ranges, which significantly limited the dispersal potential, being low for both sexes (mean fight distances-males: 101 m ± 73 SD; females: 68 m ± 80 SD). A sedentary taxon such as E. pronoe glottis does not seem to be able to avoid the pressure of resource shortage by dispersal. As a late-flying pollinator, Erebia pronoe competes seasonally for scarce resources. These are further reduced by grazing pressure and are exploited by honey bees as a superior competitor, resulting in low habitat quality and, consequently, in comparatively low abundance of E. pronoe glottis. Full article
(This article belongs to the Special Issue Biodiversity, Ecology and Conservation of Lepidoptera)
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15 pages, 2920 KB  
Article
Grazing Intensities Regulated the Effects of Seasonal Dietary Pattern on Gut Bacterial Community Composition of Sheep
by Pengzhen Li, Zhenhao Zhang, Thomas A. Monaco, Yao Dong and Yuping Rong
Microorganisms 2025, 13(6), 1392; https://doi.org/10.3390/microorganisms13061392 - 14 Jun 2025
Viewed by 601
Abstract
Gut microbiota “enterotypes” are strongly associated with diet and host health. For grazing animals, plant species richness and nutrient content of vegetation may alter the food supply and diet composition of animals. Understanding this relationship is critical to clarify the adaption of gut [...] Read more.
Gut microbiota “enterotypes” are strongly associated with diet and host health. For grazing animals, plant species richness and nutrient content of vegetation may alter the food supply and diet composition of animals. Understanding this relationship is critical to clarify the adaption of gut microbiota to changes in vegetation quantity and quality in grassland ecosystems. Here, we studied the relationship between dietary and gut microbiota composition of sheep (lambs) over a growing season in a grassland ecosystem in northern China. Variation in vegetation composition among grazing intensities was greatest in September: and sheep preferred forbs and Rosaceae throughout the grazing period in all grazing treatments, yet their preference for Fabaceae was reduced in HG treatments in September. Grazing intensity and seasonal variations in food resource availability influenced dietary patterns, which in turn affected gut bacterial community composition. Enterotype 1, dominated by Christensenellaceae_R_7_group and Clostridia_UCG_014_unclassified, predominated during the warm season (July) for both LG and HG treatments. In contrast, Enterotype 2, dominated by Escherichia_Shigella, prevailed during the cool season (September) in HG. Diversity of Enterotype 1 exceeded (p < 0.001) that of Enterotype 2. For MG, Enterotype 1 and Enterotype 2 were evenly distributed over the grazing period. Our results highlight the importance of regulating grazing intensity to maintain the balance and health of gut microbiota according to temporal changes in plant nutrients and aboveground biomass of grassland ecosystems. Full article
(This article belongs to the Special Issue Advances in Diet–Host–Gut Microbiome Interactions)
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15 pages, 2796 KB  
Article
Soil Fauna-Indicators of Ungrazed Versus Grazed Grassland Ecosystems in Romania
by Minodora Manu, Raluca Ioana Băncilă and Marilena Onete
Diversity 2025, 17(5), 323; https://doi.org/10.3390/d17050323 - 29 Apr 2025
Cited by 1 | Viewed by 796
Abstract
In order to establish the influence of grazing regime on soil fauna communities, a complex study was conducted on eight mountain grasslands in Romania. The grassland sites were grouped by management regime: ungrazed or intensely grazed by sheep. Eight environmental factors were measured, [...] Read more.
In order to establish the influence of grazing regime on soil fauna communities, a complex study was conducted on eight mountain grasslands in Romania. The grassland sites were grouped by management regime: ungrazed or intensely grazed by sheep. Eight environmental factors were measured, both abiotic (soil acidity, soil resistance at penetration, soil and air humidity, soil and air temperature, and soil electrical conductivity) and biotic (vegetation coverage). There was significant variability in the average values of these factors at the microhabitat level (between all grasslands investigated). Analysis of eighty soil samples allowed for the identification of sixteen soil fauna taxa, which constituted the database for statistical processing. The community status of these soil invertebrate faunas was mainly evaluated using three parameters: numerical abundance, taxa richness, and Shannon–Wiener index of diversity. Collembola and Oribatida were the most dominant taxa. The numerical abundance and taxa diversity recorded high values in ungrazed grasslands. Soil resistance at penetration, vegetation coverage, and soil pH influenced the numerical abundance of soil fauna communities significantly. Grassland management influenced the composition of soil invertebrates in both regimes, with Chilopoda, Staphylinidae, Diplopoda, and Enchytraeidae clearly preferring ungrazed ecosystems, whilst Mesostigmata was much commoner in grazed sites. The study revealed that correlations between the species composition of soil communities and environmental parameters under differing management regimes (ungrazed vs. grazed), demonstrated that these invertebrates can be used as bioindicators in such terrestrial ecosystems. Full article
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22 pages, 7115 KB  
Article
Identification and Feeding Characterization of Sterkiella histriomuscorum (Protozoa, Ciliophora, Hypotrichia) Isolated from Outdoor Mass Culture of Scenedesmus dimorphus
by Mengyun Wang, Pei Chen, Hongxia Wang, Qiong Deng, Xiaonan Zhang, Guoqing Yuan, Mixue Jiang, Lingling Zheng, Zixuan Hu, Zemao Gu, Denis V. Tikhonenkov and Yingchun Gong
Microorganisms 2025, 13(5), 1016; https://doi.org/10.3390/microorganisms13051016 - 28 Apr 2025
Viewed by 708
Abstract
Herbivorous protistan grazers are ubiquitous and abundant in marine and temperate freshwater environments. However, little is known about the algivorous ciliates and their feeding habits in outdoor mass algal cultures. In this study, we report on one hypotrich ciliate, identified as Sterkiella histriomuscorum [...] Read more.
Herbivorous protistan grazers are ubiquitous and abundant in marine and temperate freshwater environments. However, little is known about the algivorous ciliates and their feeding habits in outdoor mass algal cultures. In this study, we report on one hypotrich ciliate, identified as Sterkiella histriomuscorum, from the outdoor mass culture of Scenedesmus in Arizona, USA. A long-term field survey revealed that this species often occurs in Scenedesmus culture in spring and summer, and can graze very heavily on Scenedesmus cells. By isolating Sterkiella cells and then observing them via light microscopy and electron microscopy, detailed information about the morphology, ultrastructure, excystment process, and feeding characteristics of the ciliate was obtained. Specifically, it seems that S. histriomuscorum has a range of different strategies for excystment, and the sharp change in the ion concentration in the environment around the cyst results in osmotic shock, which likely facilitates the excystment. Feeding experiments revealed that S. histriomuscorum preferred to graze on chlorophytes as well as the diatom Phaeodactylum tricornutum and had no interaction with chrysophytes or cyanobacteria. Molecular phylogenetic analysis based on the SSU rRNA gene sequence indicated that both the genus Sterkiella and the species S. histriomuscorum are non-monophyletic. The information obtained from this study will help advance our understanding of the biodiversity and ecological function of S. histriomuscorum, and will also be very useful in the development of early warning systems and control measures for preventing or treating this contaminant in microalgal mass cultures. Full article
(This article belongs to the Section Molecular Microbiology and Immunology)
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33 pages, 2340 KB  
Article
Dietary Reconstruction of Pliocene–Pleistocene Mammoths and Elephants (Proboscidea) from Northern Greece Based on Dental Mesowear Analysis
by Christos Tsakalidis, George E. Konidaris, Evangelia Tsoukala and Dimitris S. Kostopoulos
Quaternary 2025, 8(2), 19; https://doi.org/10.3390/quat8020019 - 11 Apr 2025
Cited by 1 | Viewed by 1824
Abstract
Dental wear analyses of extinct animals offer key insights into their dietary preferences and in turn contribute substantially to palaeoenvironmental reconstructions, leading to more accurate interpretations about past ecosystems. This study employs dental mesowear analysis on Pliocene and Pleistocene elephants and mammoths from [...] Read more.
Dental wear analyses of extinct animals offer key insights into their dietary preferences and in turn contribute substantially to palaeoenvironmental reconstructions, leading to more accurate interpretations about past ecosystems. This study employs dental mesowear analysis on Pliocene and Pleistocene elephants and mammoths from several localities in Northern Greece (Ptolemais Basin, Mygdonia Basin, Drama Basin, and the Neapolis-Grevena Basin), aiming to classify them into three main dietary categories (browsers, mixed-feeders, grazers) and investigate potential niche partitioning. The method relies on documenting the wear pattern of molar surfaces through angle measurements on the enamel ridges, which reflect the average annual diet of the examined taxon and in turn the annual ecological conditions of the studied area. Prior to the palaeodietary study and in order to ensure the taxonomic attribution of the examined specimens, a taxonomic review was conducted which confirmed the presence of the mammoths Mammuthus rumanus, Mammuthus meridionalis (southern mammoth), and Mammuthus trogontherii (steppe mammoth), and the European straight-tusked elephant Palaeoloxodon antiquus. Dental mesowear results indicate a grazing diet for M. (cf.) rumanus, a mainly browsing diet for M. meridionalis but mixed-feeding to grazing for the subspecies Mammuthus meridionalis vestinus, a grazing one for M. trogontherii, and a wide diet spectrum for P. antiquus, including browsing, mixed-feeding and grazing, depending on the locality. This study expands our knowledge on the palaeoecology of Greek proboscideans and further highlights the importance of mesowear analysis on proboscidean teeth for palaeodietary and palaeoenviromental inferences. Full article
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18 pages, 1941 KB  
Article
Comparing Short Versus Long Persistency of Anthelmintics: Impacts on Dairy Sheep Production
by Konstantinos V. Arsenopoulos, Eleni Michalopoulou and Elias Papadopoulos
Animals 2025, 15(7), 1028; https://doi.org/10.3390/ani15071028 - 2 Apr 2025
Viewed by 774
Abstract
Introduction: Grazing dairy ewes are often heavily challenged by parasitic infections, which represent one of the most important concerns in sheep farming due to their impacts on dairy sheep production. Objectives: The objectives of the present study were (i) to compare the short [...] Read more.
Introduction: Grazing dairy ewes are often heavily challenged by parasitic infections, which represent one of the most important concerns in sheep farming due to their impacts on dairy sheep production. Objectives: The objectives of the present study were (i) to compare the short (i.e., albendazole) and long (i.e., eprinomectin) persistent effect of these anthelmintics in reducing infections by gastrointestinal nematodes (GIN) in ewes and (ii) to determine these effects on milk yield and quality in naturally infected with GIN dairy ewes. Methods: On each farm, 40 selected ewes were divided into four similar groups on Day 0 based on their fecal egg counts (eggs per gram of feces; epg) as follows: Group 1—control group; Group 2—albendazole-treated group; Group 3—pour-on eprinomectin-treated group; Group 4—injectable eprinomectin-treated group. Fecal egg counts and coprocultures were performed on Days 0, 15, 30, 45, 60 and 75. The milk yield and milk quality (i.e., fat and protein concentration and somatic cell counts) were estimated on the aforementioned occasions. Results: Eprinomectin outperformed albendazole in treating gastrointestinal nematode infections in dairy ewes. Both pour-on and injectable eprinomectin formulations provided long-lasting protection by reducing fecal egg counts (i.e., from 92.1% to 99.9%). Furthermore, eprinomectin-treated ewes exhibited increased daily milk yield and improved milk composition in terms of fat and protein concentrations and somatic cell counts. Conclusions: These findings highlight the benefits of long-persistent efficacy of eprinomectin as a preferred anthelmintic treatment for lactating dairy ewes, offering enhanced productivity and milk quality, while addressing parasite resistance concerns. Full article
(This article belongs to the Section Animal Products)
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33 pages, 1969 KB  
Article
Collaborative Adaptive Management in the Greater Yellowstone Ecosystem: A Rangeland Living Laboratory at the US Sheep Experiment Station
by Hailey Wilmer, Jonathan Spiess, Patrick E. Clark, Michelle Anderson, Amira Burns, Arica Crootof, Lily Fanok, Tracy Hruska, Bruce J. Mincher, Ryan S. Miller, William Munger, Christian J. Posbergh, Carrie S. Wilson, Eric Winford, Jessica Windh, Nicole Strong, Marlen Eve and J. Bret Taylor
Sustainability 2025, 17(7), 3086; https://doi.org/10.3390/su17073086 - 31 Mar 2025
Viewed by 1809
Abstract
Social conflict over rangeland-use priorities, especially near protected areas, has long pitted environmental and biodiversity conservation interests against livestock livelihoods. Social–ecological conflict limits management adaptation and creativity while reinforcing social and disciplinary divisions. It can also reduce rancher access to land and negatively [...] Read more.
Social conflict over rangeland-use priorities, especially near protected areas, has long pitted environmental and biodiversity conservation interests against livestock livelihoods. Social–ecological conflict limits management adaptation and creativity while reinforcing social and disciplinary divisions. It can also reduce rancher access to land and negatively affect wildlife conservation. Communities increasingly expect research organizations to address complex social dynamics to improve opportunities for multiple ecosystem service delivery on rangelands. In the Greater Yellowstone Ecosystem (GYE), an area of the western US, long-standing disagreements among actors who argue for the use of the land for livestock and those who prioritize wildlife are limiting conservation and ranching livelihoods. Researchers at the USDA-ARS US Sheep Experiment Station (USSES) along with University and societal partners are responding to these challenges using a collaborative adaptive management (CAM) methodology. The USSES Rangeland Collaboratory is a living laboratory project leveraging the resources of a federal range sheep research ranch operating across sagebrush steppe ecosystems in Clark County, Idaho, and montane/subalpine landscapes in Beaverhead County, Montana. The project places stakeholders, including ranchers, conservation groups, and government land managers, in the decision-making seat for a participatory case study. This involves adaptive management planning related to grazing and livestock–wildlife management decisions for two ranch-scale rangeland management scenarios, one modeled after a traditional range sheep operation and the second, a more intensified operation with no use of summer ranges. We discuss the extent to which the CAM approach creates opportunities for multi-directional learning among participants and evaluate trade-offs among preferred management systems through participatory ranch-scale grazing research. In a complex system where the needs and goals of various actors are misaligned across spatiotemporal, disciplinary, and social–ecological scales, CAM creates a structure and methods to focus on social learning and land management knowledge creation. Full article
(This article belongs to the Section Sustainable Management)
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29 pages, 5393 KB  
Article
Spatial Distribution Patterns, Environmental Drivers, and Hotspot Dynamics of the European Rabbit on a Mediterranean Island: Implications for Conservation and Management
by Yiannis G. Zevgolis, Foto Konsola, Athanasia-Zoi Bouloutsi, Niki-Nektaria Douskou, Ioanna Emmanouilidou, Maria-Alexandra Kordatou, Anastasia Lekka, Maria-Eirini Limnioti, Maria Loupou, Despoina Papageorgiou, Michailia-Theodora Papamakariou, Eleni Tsiripli, Panagiotis Tzedopoulos, Christos Xagoraris, Alexandros D. Kouris and Panayiotis G. Dimitrakopoulos
Biology 2025, 14(3), 225; https://doi.org/10.3390/biology14030225 - 20 Feb 2025
Cited by 1 | Viewed by 1914
Abstract
The European rabbit (Oryctolagus cuniculus) presents a significant conservation and management challenge in Greece. While it has been listed in national biodiversity assessments, its population dynamics on the island of Lemnos demonstrate the characteristics of a highly adaptable and rapidly expanding [...] Read more.
The European rabbit (Oryctolagus cuniculus) presents a significant conservation and management challenge in Greece. While it has been listed in national biodiversity assessments, its population dynamics on the island of Lemnos demonstrate the characteristics of a highly adaptable and rapidly expanding species, exerting substantial ecological and economic impacts. Addressing this issue requires a spatially explicit understanding of its distribution patterns and habitat preferences, particularly given its extensive population growth over the past three decades. To this end, we conducted 40 field surveys across the island, documenting 1534 presence records of the species. We applied Kernel Density Estimation, Getis-Ord Gi *, and Anselin Local Moran’s I to identify the spatial distribution patterns and significant hotspots. A spatial lag model was used to quantify hotspot intensity and clustering dynamics, while abiotic, biotic, and anthropogenic factors were analyzed to assess habitat associations. Our results revealed that rabbit hotspots are predominantly concentrated in fertile lowland agroecosystems, with nearly 60% of high-density areas overlapping conservation zones. Soil and field conditions, grazing-supporting landscapes, and arable and subsidized agricultural areas emerged as significant predictors of O. cuniculus presence. The observed spatial dependencies indicated that while hotspot intensities and clustering dynamics are influenced by the conditions in neighboring areas, habitat characteristics remain fundamental in shaping their distribution, highlighting the broader landscape-scale spatial patterns affecting rabbit populations. These findings underscore the necessity of adopting spatially informed management strategies that mitigate agricultural impacts while accounting for interconnected spatial dynamics, providing a foundation for informed decision-making to manage rabbit populations while balancing conservation and agricultural priorities. Full article
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27 pages, 12750 KB  
Article
Sustainable Intensification of the Montado Ecosystem: Evaluation of Sheep Stocking Methods and Dolomitic Limestone Application
by Emanuel Carreira, João Serrano, Shakib Shahidian, Paulo Infante, Luís L. Paniagua, Francisco Moral, Luís Paixão, Carlos Pinto Gomes, José Lopes de Castro, Mário de Carvalho and Alfredo F. Pereira
Sustainability 2025, 17(1), 363; https://doi.org/10.3390/su17010363 - 6 Jan 2025
Viewed by 1375
Abstract
The objective of this study was to determine how application of dolomitic limestone and stocking methods (continuous stocking or deferred stocking) affect the soil compaction, sheep grazing location, height, and nutritional value of pastures when the pasture growth rate is at its maximum. [...] Read more.
The objective of this study was to determine how application of dolomitic limestone and stocking methods (continuous stocking or deferred stocking) affect the soil compaction, sheep grazing location, height, and nutritional value of pastures when the pasture growth rate is at its maximum. A 4 ha field at Mitra farm—University of Évora—was divided into four plots: P1 and P2—without application of dolomitic limestone, continuous stocking (CS), and deferred stocking (DS), respectively—and P3 and P4—with application of dolomitic limestone, DS (2.3 AUE), and CS (1 AUE), respectively. In DS, animals were placed and removed from the plots depending on the height of the pasture (entry ≥ 10 cm; removal ≤ 5 cm). Throughout the pasture’s vegetative cycle, several measurements of pasture height and cut were carried out. From the beginning of March to the beginning of June, animal behavior was observed (animals’ activity grazing and location) by trained observers through binoculars on six dates. The results show the following: (i) the application of dolomitic limestone combined with CS provided higher values of pasture height; (ii) there were no significant differences in pasture quality between treatments; (iii) DS led to 50% more sheep grazing days that CS; (iv) there were no significant differences in soil compaction between CS and DS; and (v) the stocking methods and the application of dolomitic limestone did not seem to change the grazing pattern between treatments. This study constitutes a basis to support more informed decisions by agricultural managers and may also contribute to maintaining balance in the Montado ecosystem, as well as increasing the efficiency of livestock production systems based on rainfed pastures. Full article
(This article belongs to the Section Sustainable Agriculture)
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24 pages, 2773 KB  
Article
Integrating Mixed Livestock Systems to Optimize Forage Utilization and Modify Woody Species Composition in Semi-Arid Communal Rangelands
by Mhlangabezi Slayi and Ishmael Festus Jaja
Land 2024, 13(11), 1945; https://doi.org/10.3390/land13111945 - 18 Nov 2024
Cited by 5 | Viewed by 1371
Abstract
Communally owned rangelands serve as critical grazing areas for mixed livestock species such as cattle and goats, particularly in the arid and semi-arid regions of Southern Africa. This study aimed to evaluate the nutritional composition and woody species composition of communal rangelands where [...] Read more.
Communally owned rangelands serve as critical grazing areas for mixed livestock species such as cattle and goats, particularly in the arid and semi-arid regions of Southern Africa. This study aimed to evaluate the nutritional composition and woody species composition of communal rangelands where cattle and goat flocks graze together and to investigate the influence of grazing intensity on vegetation dynamics. Vegetation surveys were conducted across varying grazing intensities to assess species richness, biomass, and dietary preferences, while soil properties were analyzed to determine their interaction with vegetation attributes. Stepwise regression and path analyses were used to explore the relationships between soil characteristics, vegetation structure, and livestock dietary choices. The results revealed that high grazing pressure significantly reduced grass biomass (p = 0.003) and woody species density (p = 0.007) while increasing shrub cover (p = 0.018). Nutritional analysis indicated that goats preferred woody shrubs, which contributed 42.1% of their diet compared to 27.8% for cattle (p = 0.008). Regression analysis further showed that soil organic carbon (p = 0.002) and tree height (p = 0.041) were strong predictors of shrub cover. Seasonal variation significantly affected forage availability and nutritional content, with higher crude protein levels recorded during the wet season (p = 0.007). These findings suggest that grazing management strategies should be tailored to the distinct forage needs of cattle and goats to maintain the productivity and ecological stability of communal rangelands. A holistic approach that considers livestock dietary preferences, vegetation composition, and soil health is essential for sustainable rangeland management in mixed-species grazing systems. Full article
(This article belongs to the Section Land–Climate Interactions)
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22 pages, 5341 KB  
Article
Multi-Annual Study of Eriogaster catax (Linnaeus, 1758) (Lepidoptera, Lasiocampidae) Oviposition Strategy in Transylvania’s Largest Population: Key Insights for Species Conservation and Local Land Management
by Cristian Sitar, Geanina Magdalena Sitar, Angela Monica Ionică, Vladimír Hula, Lukáš Spitzer, Alina Simona Rusu and László Rakosy
Insects 2024, 15(10), 794; https://doi.org/10.3390/insects15100794 - 12 Oct 2024
Viewed by 1487
Abstract
This study provides new insights into the oviposition strategy of Eriogaster catax (Linnaeus, 1758) (Lepidoptera, Lasiocampidae), an endangered species of moth found in semi-natural habitats within agricultural landscapes. Protected under various European directives and listed as Data Deficient by the IUCN, E. catax [...] Read more.
This study provides new insights into the oviposition strategy of Eriogaster catax (Linnaeus, 1758) (Lepidoptera, Lasiocampidae), an endangered species of moth found in semi-natural habitats within agricultural landscapes. Protected under various European directives and listed as Data Deficient by the IUCN, E. catax inhabits warmer regions of the Western Palearctic. Despite noted geographic variations in its ecological preferences, few studies have statistically significant data on its ecology. Our six-year study, conducted within the largest known population of E. catax. in Romania, reveals critical data on its oviposition preferences, including the species’ tendency to utilize Prunus spinosa L. and Crataegus monogyna Jacq. shrubs at an average height of 80.48 ± 34.3 cm, with most nests placed within the 41–80 cm range and containing an average of 186 ± 22 eggs. The study also addresses the species’ vulnerability to human activities such as bush trimming, agricultural burning, and uncontrolled grazing, particularly due to its low oviposition height. These findings underscore the negative impact of overgrazing and burning practices, particularly when conducted on a large scale, on the conservation of E. catax. The detailed ecological requirements identified in this study are essential for developing effective conservation strategies and habitat management practices. Furthermore, the study highlights the importance of local community involvement and public education in raising awareness about biodiversity and the conservation of endangered species. Full article
(This article belongs to the Section Insect Ecology, Diversity and Conservation)
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24 pages, 3731 KB  
Article
The Dynamics of Trophic Cascades on Phytoplankton Induced by Mesozooplankton in Coastal Water, Daya Bay, Northern South China Sea
by Bingqing Liu, Mianrun Chen and Chunsheng Wang
Microorganisms 2024, 12(10), 1982; https://doi.org/10.3390/microorganisms12101982 - 30 Sep 2024
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Abstract
Daya Bay, a semi-enclosed bay in the northern South China Sea and to the east of the Pearl River Estuary, is rich in biological resources and diverse habitats. Current research on mesozooplankton in Daya Bay has mainly focused on aspects such as species [...] Read more.
Daya Bay, a semi-enclosed bay in the northern South China Sea and to the east of the Pearl River Estuary, is rich in biological resources and diverse habitats. Current research on mesozooplankton in Daya Bay has mainly focused on aspects such as species composition, biomass, and biodiversity in the zooplankton community. However, there is limited research on the top-down effects of mesozooplankton on prey communities. This study conducted seasonal in-situ cultivation experiments from 2015 to 2017. By combining mesozooplankton grazing experiments and microzooplankton dilution experiments, the mesozooplankton clearance rate and trophic cascading effect on low trophic levels were calculated. Results showed evident mesozooplankton selective feeding behavior and corresponding trophic cascades with seasonal variations, these being significantly higher in the spring and summer and lower in the autumn and winter. Different sizes of phytoplankton showed significant differences; large-sized phytoplankton received high feeding rates but low trophic cascades by mesozooplankton, while the opposite was true for small-sized phytoplankton. Trophic cascades contribute in three ways: offsetting direct grazing mortality, changing prey community structure via its effects on different phytoplankton sizes, and reducing ciliate grazing impacts at an average of 14.4 ± 7.8%, maintaining around 70% ciliate grazing impacts in nature. The composition of mesozooplankton was the primary reason for explaining feeding preferences, including size selectivity and omnivory. For instance, high cladoceran abundance caused high feeding rates while, on the other hand, high omnivorous copepods abundance caused high trophic cascades on small-sized phytoplankton. General additive model (GAM) analysis revealed that the changes in trophic cascades were highly dependent on temperature, ciliate abundance, mesozooplankton feeding rates on ciliates, and ciliate feeding rates on phytoplankton. The significance of this study lies in its contribution to providing valuable insights into the role of mesozooplankton in the marine food web and their impact on lower trophic levels. In addition, the findings can help inform the management and conservation of marine ecosystems, as well as guide future research in this field. Full article
(This article belongs to the Special Issue Marine Microorganisms and Ecology)
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