Search Results (159)

Shallot (Allium hirtifolium Boiss.) is of considerable nutritional and medical significance due to its strong antioxidant properties; however, no nanophytotoxicity studies have assessed whether the use of nanofertilizers would improve shallot performance, micronutrient iron (Fe) enrichment, and yield in semi-arid regions. Herein, we evaluated the effects of magnetite nanoparticles (nFe₃O₄) on shallot grown for a full lifecycle in two semi-arid regions through bulb-priming followed by foliar application and compared them with conventional ferrous sulfate (FeSO₄) fertilizer and untreated control. Our results showed remarkable cellular adaptations to semi-arid climate upon nFe₃O₄ treatment as leaves displayed thickened cell walls, distinct chloroplasts featuring organized thylakoid grana and stroma, normal mitochondria, abundant starch grains, and plastoglobuli around chloroplasts compared to FeSO₄ or untreated control. At 900 mg/L nFe₃O₄, chlorophyll-a, chlorophyll-b, and carotenoid increased by 27–55%, 108–126%, and 77–97%, respectively, compared to FeSO₄ applied at recommended field rate (1800 mg/L). Significant increments in bulb diameter (38–39%) and sister bulb number (300–500%) were observed upon 900 mg/L nFe₃O₄ treatment compared to FeSO₄ (1800 mg/L) and control. Furthermore, with 900 mg/L nFe₃O₄ treatment, total phenol, flavonoids, and Fe in bulbs increased by 27–46%, 29–73%, and 486–549%, respectively, compared to FeSO₄ (1800 mg/L). These findings demonstrate that bulb-priming followed by foliar application of 900 mg/L of nFe₃O₄ could significantly promote cellular adaptation, thereby improving photosynthetic efficiency, bulb yield, antioxidant activities, and Fe biofortification in shallot, and may serve as a novel approach for improving shallot production in semi-arid regions. Full article

Oats (Avena sativa L.) are a staple grain and forage crop with substantial market demand. In China, they are the second most-imported forage grass, only after alfalfa (Medicago sativa L.). Enhancing the salt tolerance of oats to facilitate their cultivation in saline areas can thereby increase forage yield and promote the utilization of saline land, which constitutes an important reserve land resource in China. This study aimed to identify the bacterial strain Bacillus sp. LrM2 (hereafter referred to as strain LrM2) to determine its precise species-level classification and evaluate its effects on oat photosynthesis and growth under salt stress through indoor pot experiments. The results indicated that strain LrM2, capable of urease production and citrate utilization, was identified as Bacillus mojavensis. The strain LrM2 had a positive effect on shoot and root growth of oats under 100 mM NaCl stress conditions. Strain LrM2 inoculation modulated osmotic stress in oats under 100 mM NaCl stress by significantly increasing soluble sugar and decreasing proline content in leaves. It inhibited Na⁺ uptake and promoted K⁺ absorption in the roots, thereby reducing Na⁺ translocation to the leaves and mitigating ionic toxicity. Inoculation with strain LrM2 significantly increased photosynthetic pigment content (chlorophyll a, carotenoids), improved gas exchange parameters (stomatal conductance, transpiration rate, net rate of photosynthesis), enhanced PSII photochemical efficiency (maximum quantum yield, coefficient of photochemical quenching, actual photosynthetic efficiency of PSII, electron transfer rate), and reduced the quantum yield of non-regulated energy dissipation. These improvements, coupled with increased relative water content and instantaneous water use efficiency, thereby collectively enhanced the overall photosynthetic performance. In conclusion, strain LrM2 represents a promising bio-resource for mitigating salt stress and promoting growth in oats, with direct applications for developing novel biofertilizers and sustainable agricultural strategies. Full article

The use of Bacillus spp. in combination with mineral fertilizers represents a sustainable alternative to conventional agricultural practices. This study evaluated the effects of inoculation with Bacillus amyloliquefaciens BV03 (Ba) on corn fertilized with phosphorus (P) sources of different solubilities. Two experiments were conducted under greenhouse conditions in a completely randomized design, following a 2 (without and with Ba) × 4 [control (without P, –P), triple superphosphate (TSP), Bayóvar natural phosphate (BNP), and Pratápolis natural phosphate (PNP)] factorial arrangement. Plant growth parameters, chlorophyll a fluorescence, gas exchange, photosynthetic pigments, nutritional status, biomass accumulation, and grain yield were assessed. Corn responses to Ba inoculation varied with P source and season. Inoculation with Ba, Ba + TSP, and Ba + BNP at sowing enhanced biometric traits (height, stem diameter, and leaf area); physiological parameters (F_v’/F_m’, ΦPSII, ETR, E, g_s, WUE); biochemical variables (Chl a, Chl b, and carotenoids); nutritional contents (N, P, K, Ca, and Mg); and yield traits. Overall, our results highlight the potential of Bacillus amyloliquefaciens BV03, alone or in combination with triple superphosphate or Bayóvar natural phosphate, as a sustainable alternative for phosphorus fertilization to improve corn growth and development. Full article

In agricultural production, Deoxynivalenol (DON) generally exists and contaminates wheat, corn, and other grains, causing intestinal damage and immunotoxicity. Lycopene (LYC), an antioxidant, anti-inflammatory carotenoid, is mainly found in red fruits such as tomatoes and has been investigated for its great medicinal advantages. This study aimed to investigate the protective effect of LYC against DON-induced enterotoxicity. Our findings demonstrated that incubation of IPEC-J2 cells with 0.5 μM DON for 24 h caused intestinal barrier impairment and oxidative stress induction, which subsequently led to increased secretion of pro-inflammatory factors (TNF-α, IL-1β, IL-18, and IL-6) and decreased secretion of the counterregulatory factor (IL-10). Furthermore, DON ultimately induced NLRP3 inflammasome activation through the stimulation of the MAPK/NF-κB pathway. It is worth mentioning that the above changes were reversed after adding 30 μg/mL of LYC to DON-exposed IPEC-J2 cells. In addition, further experiments confirmed that ERK activator (4-Methylbenzylidene camphor, 4-MBC) eliminated the positive effect of LYC on alleviating enterotoxicity induced by DON in IPEC-J2 cells. In addition, further experiments confirmed that 4-MBC eliminated the positive effect of LYC on alleviating enterotoxicity induced by DON. In general, our study certified that ERK is a therapeutic target for LYC protecting DON-induced intestinal barrier dysfunction and NLRP3 inflammasome activation. Full article

Improving rye (Secale cereale) yield under increasing climatic stress remains a major challenge for sustainable cereal production. We examined whether early-vegetative physiological, biochemical, and molecular traits can predict final grain yield in hybrid-breeding components. Across three consecutive seasons, 14 genotypes were evaluated under controlled cold-greenhouse conditions for chlorophyll fluorescence, pigment content, hydrogen peroxide (H₂O₂), salicylic acid (SA) levels, and the expression of selected antioxidant and defence-related genes, and these traits were related to yield components. Across years, photosynthetic efficiency (F_v/F_m, Rfd), chlorophyll content, and foliar H₂O₂ emerged as the most consistent predictors of kernel mass, spike number, and kernel number. In contrast, non-photochemical quenching, SA, and carotenoid contents showed weak or inconsistent relationships with yield. These findings indicate that light-harvesting capacity, PSII performance, and oxidative balance are central to reproductive success in rye. The stability of these trait–yield correlations across three seasons provides the basis for a physiological robustness index for hybrid rye, with predictive models achieving accuracies up to R = 0.51. This work demonstrates the potential of using a compact set of early-stage, high-throughput physiological traits to accelerate selection for stress-resilient, high-yielding rye cultivars. Full article

Due to the intensive cultivation of various crops, the surface soil layer is depleted. This leads to a decrease in fertility, losses of organic matter and nutrients, and an overall decrease in soil health. We aimed to investigate the role of green manure application and organic fertilization on winter fodder barley (Hordeum vulgare L., Zemela cult.) in terms of agronomic and soil parameters. The cultivation was carried out in two fields, the predecessors of which were oats–vetch green manure (field 1) or fallow (field 2). In each field, five treatments were prepared: a control without fertilization, mineral fertilization, vermicompost, mineral fertilizer + vermicompost, and biochar. The green manure incorporation led to a decrease in grain yield of barley by 10.8–20.0% depending on the treatment. A similar tendency was observed for the rest of the studied agronomic parameters (thousand-grain mass, hectolitre weight, ear number, plants per hectare). Additionally, the vermicompost application had the most substantial effect, accounting for a 20.1% increase compared to the control, while the smallest was expressed by biochar—1.6%. Nevertheless, the photosynthesis intensity was higher in treatments after green manure. The microbiome’s activity was boosted in the vermicompost treatments, while amino acids, carboxylic acids, and polymers were the most fully metabolised compounds by the soil communities. In conclusion, the type of predecessor influenced mainly grain protein, carotenoids, and chlorophyll contents, as well as microbial activities, respiration, and dehydrogenase, while the fertilization impacted primarily on soil water and organic content, total soil N, and photosynthetic pigments of barley plants. Full article

Cordyceps militaris (C. militaris) is a medicinal fungus renowned for its diverse therapeutic properties, largely attributed to bioactive compounds such as cordycepin, polysaccharides, adenosine, D-mannitol, carotenoids, and ergosterol. However, the production and composition of these metabolites are highly influenced by cultivation conditions, highlighting the need for systematic optimization strategies. This review synthesizes current findings on how nutritional factors—including carbon and nitrogen sources, their ratios, and trace elements—and environmental parameters such as oxygen availability, pH, temperature, and light regulate C. militaris metabolite biosynthesis. The impacts of solid-state fermentation (using grains, insects, and agro-industrial residues) and liquid state fermentation (submerged and surface cultures) are compared, with attention to their roles in mycelial growth, fruiting body formation, and secondary metabolite production. Special emphasis is placed on mixed grain–insect substrates and light regulation, which have emerged as promising methods to enhance cordycepin accumulation. Beyond summarizing advances, this review also identifies key knowledge gaps that must be addressed: (i) the incomplete understanding of metabolite regulatory networks, (ii) the absence of standardized cultivation protocols, and (iii) unresolved challenges in scale-up, including oxygen transfer, foam control, and downstream processing. We propose that future research should integrate multi-omics approaches with bioprocess engineering to overcome these limitations. Collectively, this review highlights both current progress and remaining challenges, providing a roadmap for advancing the sustainable, scalable, and application-driven production of bioactive compounds from C. militaris. Full article

Objectives: The rapid aging of the U.S. population has raised concerns about age-related cognitive decline and Alzheimer’s disease. Therefore, we aimed to characterize diet quality, nutrient intake, and to examine the associations between specific dietary components and cognitive performance in older adults. Design: Cross-sectional observational study. Setting: Community-based recruitment. Participants: Data from 72 community-dwelling adults aged 65 years and older was analyzed. Measurements: Cognitive performance was assessed using subtests from the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD) battery, evaluating episodic memory (Word List Memory/Recall/Recognition), visuospatial skills (Constructional Praxis), and executive function (Verbal Fluency). A composite cognitive score was calculated from memory and visuospatial subtests. Habitual dietary intake was evaluated using structured 24-h recalls to calculate nutrient intake and the Healthy Eating Index score, supplemented by the Short HEI questionnaire. Demographics, health history, depressive symptoms (Patient Health Questionnaire-9), and sleep quality (Pittsburgh Sleep Quality Index) were also collected. Results: Participants demonstrated suboptimal diet quality (mean HEI score 62.9 ± 10.69; recommended >80), with only 9.7% meeting fiber recommendations, 11% meeting calcium or vitamin A recommendations, and 1.4% meeting vitamin D requirements. In bivariate comparisons, higher cognitive performance was observed in younger participants (75.5 vs. 79.5 years; p < 0.01) and females (78% vs. 50%; p = 0.024). Regression models identified significant positive associations between cognitive scores and intakes of dietary fiber (p = 0.007), unsaturated fats (mono- and polyunsaturated; p = 0.012–0.033), protein (p = 0.018), carotenoids (α-carotene, p = 0.001; β-carotene, p = 0.026; lutein + zeaxanthin, p = 0.016), vitamins A (p = 0.044) and E (p = 0.034), and minerals including magnesium (p = 0.006), potassium (p = 0.004), copper (p = 0.008), zinc (p = 0.024), and calcium (p = 0.035). Refined grain intake was inversely associated with cognition (p = 0.011). Conclusions: In this population, dietary components like fiber and micronutrients were positively associated with better cognitive function, and the overall nutrient intake shortfalls observed highlight the need for targeted dietary interventions to support healthy brain aging. Therefore, this work advances our understanding by highlighting potential modifiable nutritional targets for clinical trials focused on delaying or preventing cognitive decline. Full article

Given the critical role of manganese (Mn) as an essential micronutrient in wheat growth and development and the high efficiency of foliar fertilization in optimizing nutrient uptake and improving crop quality, this study aimed to elucidate the regulatory effects of exogenous manganese sulfate application on wheat grain yield and carotenoid accumulation. Methods: Field experiments were conducted from 2022 to 2024 at the Shuitou Experimental Station of the Cotton Research Institute, Shanxi Agricultural University (35°11′ N, 111°05′ E), using the wheat cultivar ‘Jinmai 110’. Foliar applications of manganese sulfate were administered at concentrations of 0.5 g/kg, 1.0 g/kg, and 1.5 g/kg, with water serving as the control (CTRL). Spraying was conducted on the upper canopy during the flowering and grain-filling stages, applied every 7 days for a total of three times. Samples for transcriptomic analysis were collected within 24 h of the final application. At maturity, yield-related traits and grain carotenoid contents were assessed. Results: Foliar application of 1.0 g/kg MnSO₄ significantly enhanced both grain yield and carotenoid content in wheat. Transcriptome sequencing revealed that treatment with 1.0 g/kg manganese sulfate (M2) resulted in 4761 differentially expressed genes (DEGs), including 2933 upregulated and 1828 downregulated genes, relative to CTRL. Gene Ontology (GO) analysis showed that in the M2 vs. CTRL comparison, 819 GO terms were significantly enriched among upregulated DEGs and 630 among downregulated DEGs. Specifically, upregulated genes were associated with 427 biological process terms and 299 cellular component terms, while downregulated genes were linked to 361 biological processes and 211 cellular components. Enriched functions primarily included cellular processes, metabolic processes, catalytic activity, and binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 809 annotations for upregulated DEGs and 330 for downregulated DEGs, mainly related to photosynthesis, carotenoid biosynthesis, phenylpropanoid biosynthesis, and plant hormone signal transduction. In total, 43,395 alternative splicing (AS) events were identified from 17,165 genes, including 445 upregulated and 319 downregulated AS events, primarily enriched in photosynthesis and plant hormone-related pathways. Conclusion: Foliar application of manganese sulfate significantly modulates gene expression in wheat grains, thereby improving both yield and carotenoid accumulation. Key biological processes affected include photosynthesis, plant hormone signal transduction, and the carotenoid biosynthetic pathway. The interactions among these regulatory networks constitute a complex molecular mechanism through which exogenous Mn influences agronomic traits. These findings provide mechanistic insights and practical implications for enhancing wheat productivity and nutritional quality through foliar manganese application. Full article

Hairless canary seed (Phalaris canariensis L.) can play significant roles in human health and nutrition due to its unique nutrient profile. It belongs to the Gramineae family similar to common cereal grains like wheat, rice and corn. On the other hand, the traditional canary seed is characterized by the presence of silicified spicules or hairs on the hulls of the kernel that could pose health hazards to humans. The hairless canary seed was developed in Canada by a conventional breeding program to mitigate the health concerns associated with the silicified hairs. The hairless grain is silica free, i.e., totally glabrous, and is granted regulatory food approvals by Health Canada and US-FDA. The hairless grain holds a great potential as a whole grain functional food ingredient due to its unique nutritional and functional attributes. As a cereal grain, it is rich in protein that is non-gluten and exceptionally high in tryptophan and bioactive peptides. The grain also contains reasonable amounts of carotenoids, polyphenols, and healthy unsaturated oil. Because of these special characteristics, it is considered a promising nutritious and therapeutic food. This review provides insights into the potential of hairless canary seed as a functional ingredient in products designed to mitigate oxidative stress, diabetes and celiac disease and/or to improve vision and cognition. Full article

Lead (Pb) and cadmium (Cd) severely impair rice growth, yield, and grain quality. This study assessed the role of exogenous gamma-aminobutyric acid (GABA) in mitigating Pb and Cd toxicity in aromatic rice ‘Guixiangzhan’. Treatments included the control (no Pb, Cd, or GABA), GABA (1 mM), Pb (800 mg/kg of soil)+GABA, Cd (75 mg/kg of soil)+GABA, Pb+Cd+GABA, Pb, Cd, and Pb+Cd without GABA. GABA improved chlorophyll and carotenoid, protein, proline and GABA contents whilst reducing oxidative stress under Pb/Cd toxicity. GABA application regulated antioxidant enzyme activities, net photosynthesis, and gas exchange, while its effects on nitrate reductase and glutamine synthetase were variable. Compared with Pb+Cd, the grain yields were 34.03%, 31.94%, 15.88%, 24.86%, and 17.32% higher in (Pb, Cd, Pb+Cd)+GABA, Pb, and Cd treatments, respectively. Furthermore, GABA reduced Pb and Cd accumulation in aboveground parts, while Ca, Mg, Fe, Cu, Zn, and Mn levels varied across treatments. Cd translocation was more from root-to-leaves, while Pb translocation was more from leaves-to-grains. Grain Pb and Cd positively correlated with their root, stem, and leaf contents but negatively with mineral nutrients. Overall, exogenous GABA mitigated Pb and Cd toxicity in aromatic rice. Full article

Purple totomoxtle (maize husk) in native maize represents a phenotypic trait of cultural and agronomic significance within traditional Mesoamerican agroecosystems. This study evaluated the phenotypic expression of anthocyanins in vegetative and reproductive tissues of ten native maize genotypes, including inter-parental crosses derived from both pigmented and non-pigmented lines. Field trials were conducted under rainfed conditions in Chiapas, Mexico. Visual and quantitative assessments included pigmentation intensity, chlorophyll and carotenoid content, ear traits and appearance, grain health, and yield performance. Genotypes exhibiting the purple phenotype showed consistent pigment accumulation in stems, nodes, leaf sheaths, tassels, and bracts (totomoxtle), with statistically significant differences compared to non-pigmented controls. Anthocyanin content in totomoxtle increased by 30% during late developmental stages, whereas chlorophyll and carotenoid levels peaked during early vegetative growth. Pigmented materials displayed healthier grain, enhanced ear appearance, and higher yields, with the JCTM × LLMJ cross reaching 6.60 t ha⁻¹. These findings highlight the functional value of purple totomoxtle and its potential in agroecological programs aimed at resilience, genetic conservation, and integral resource utilization, providing useful criteria such as stable pigment expression and superior yield to guide sustainable reproduction strategies. Full article

Cucumber mosaic virus (CMV) is a destructive viral pathogen of vegetables, fruits, grains, and ornamentals across the globe. This study investigated the comparative antiviral efficacy of chitosan–salicylic acid nanocomposite (Ch/SA NC) and salicylic acid (SA) against CMV in cucumber plants. Transmission electron microscopy (TEM) analyses revealed that Ch/SA NCs can aggregate on the viral coat protein surface, suggesting direct nanoparticle–virus interaction. Greenhouse trials showed that Ch/SA NC, particularly at 90 ppm applied 24 h before CMV inoculation, was the most effective treatment in reducing disease severity and viral load. SA at the same concentration also conferred significant protection when used prophylactically. An RT-PCR analysis confirmed suppression or complete silencing of CMV coat protein gene expression, especially Ch/SA NC-treated plants. Both treatments significantly enhanced the physiological condition of infected plants, including restoration of chlorophyll a, chlorophyll b, and carotenoids, and elevated levels of total phenolics, flavonoids carbohydrates, and proteins. In addition, they boosted the key antioxidant enzymes activities (POX, PPO, SOD) and improved vegetative growth indicators such as plant height, fruit fresh weight, and number of fruits per plant. These results indicate that Ch/SA NC and SA not only inhibit CMV replication but also stimulate host defense responses, improving overall plant health. The strong antiviral effect is likely due to the dual action of Ch/SA NC: direct virus binding and induction of systemic acquired resistance (SAR). Given their efficacy and eco-friendly nature, especially the Ch/SA NC, these treatments offer a promising strategy for integrated viral disease management. Future studies should investigate long-term environmental safety, molecular mechanisms, and field-level applicability. Full article

Chemical fertilizers pose significant risks to both human health and the environment. To investigate the effect of substituting nitrogen fertilizer with vermicompost tea on wheat yield, shoot chemical constituents, and grain quality under clay-loam soil conditions, two field experiments were conducted at the Faculty of Agriculture, Cairo University, Egypt, during the winter seasons of 2021–2022 and 2022–2023. A split-plot design in randomized complete blocks with three replications was employed. Vermicompost tea was assigned to the main plots, while wheat cultivars were assigned to the subplots. The cultivars were evaluated under four treatments involving partial substitution of mineral nitrogen (recommended dose of nitrogen (RDN%, 190 kg N ha⁻¹): a control (90% of RDN + 25 kg vermicompost tea), 80% of RDN + 37.5 kg vermicompost tea, and 70% of RDN + 50 kg vermicompost tea. Nitrogen fertilizer (RDN%) was applied at rates of 190 (control), 170 (90%), 150 (80%), and 130 (70%) kg N ha⁻¹. The results indicated that partially substituting mineral nitrogen with vermicompost tea significantly increased grain weight/Ha, chlorophyll A, chlorophyll B, carotenoids, nitrogen, phosphorus (P), and potassium (K) content in shoots, as well as ash, crude protein, crude fiber, total sugar, and N, P, and K content in wheat grains. The grain weight/Ha of the Sakha-95, Giza-171, and Sads-14 cultivars increased by 38.6%, 33.5%, and 39.3%, respectively, when treated with 70% RDN + 50 kg vermicompost tea. The combination of the Sads-14 cultivar and 70% RDN + 50 kg vermicompost tea resulted in the highest values for grain weight/ha (9.43 tons ha⁻¹), chlorophyll A (1.39 mg/g), chlorophyll B (1.04 mg/g), N (5.08%), P (1.63%), and P (2.43%) content in shoots. The same combination also improved ash (2.89%), crude fiber (2.84%), and K (6.05%) content in grains. In conclusion, the application of vermicompost tea in conjunction with chemical fertilizers offers a viable alternative to using chemical fertilizers alone, promoting sustainable agricultural practices and improving wheat production. It is recommended that mineral nitrogen fertilizer be partially replaced with vermicompost tea to enhance both the productivity and grain quality of wheat while minimizing environmental pollution. Full article

Foliar fertilization is an effective practice that improves both the yield and quality of maize, a crop with high and specific micronutrient demands. This study hypothesized that foliar application of Fe, Cu, Mn, Mo, Zn and B would improve grain size and quality in GS210 maize compared to the control. The single-factor field experiment was conducted in 2023–2024 on Haplic Cambisol (Eutric) soil, under a variety of meteorological conditions. The application of Zn and B fertilizers significantly increased the soil plant analysis development (SPAD) index. Yield components (number of grains per ear, thousand-grain weight) and grain yield increased significantly following Zn foliar application compared to the control. Zn application increased grain yield by 0.59 t ha⁻¹ and 0.49 t ha⁻¹ in 2023 and 2024, respectively. Smaller but beneficial effects were observed with Cu and B applications. In contrast, the effects of fertilization with other micronutrients (Fe, Mn, Mo) were less pronounced than anticipated. Biochemical analyses revealed that foliar fertilization with Fe, Cu and Mo increased total phenolic content and antioxidant capacity, while Fe and Mo enhanced carotenoid accumulation, and Cu and B significantly influenced grain color parameters. The study highlights the potential of foliar fertilization to improve maize performance and grain quality, despite possible antagonisms between micronutrients. Full article