Search Results (829)

Milk titratable acidity is a key indicator of raw milk freshness and quality, but its variation across different dairy animal species remains incompletely characterized. Based on 16,984 raw milk samples from eight dairy animal species (Holstein cow, goat, buffalo, camel, sheep, yak, donkey, and horse) collected within a retrospective raw milk quality monitoring framework in China from 2016 to 2024, this study provides a large-scale descriptive comparison of milk titratable acidity across species. Distinct titratable acidity profiles were observed among species, with camel and yak milk showing relatively high values, sheep, Holstein, and buffalo milk exhibiting intermediate values, and donkey and horse milk presenting markedly low values. Calendar-season-associated patterns also differed among species. Correlations between titratable acidity and milk components varied by species, with relatively stronger positive associations with protein and solids-not-fat (SNF) in several ruminant milks, suggesting that milk composition may contribute to differences in titratable acidity. However, because this study was based on an unbalanced observational dataset with limited animal-level, farm-level, feeding, management, physiological, and environmental metadata, these observations should be interpreted as descriptive and exploratory patterns rather than causal biological mechanisms. This dataset provides preliminary reference information for future studies on species-associated variation in raw milk titratable acidity and for discussions on species-specific raw milk quality evaluation. Full article

Dairy goat farming is a niche but relevant livestock system in alpine regions, yet its economic viability and environmental performance remain poorly quantified. This study assessed the relationship between profitability and environmental impacts in dairy goat farms in South Tyrol (Northern Italy). Data were collected from ten alpine dairy goat farms through on-farm interviews and accounting records and exploratorily analyzed using full-cost accounting and life cycle assessment (LCA). Given the small and purposive sample, all findings should be interpreted as preliminary and hypothesis-generating rather than statistically representative. Environmental impacts were evaluated from cradle to farm gate using two functional units: 1 kg of fat- and protein-corrected milk (FPCM) and 1 ha of agricultural land. Farm income per kg FPCM was highly variable, ranging from −€1.10 to €2.50, and depended strongly on herd size and subsidies. Average global warming potential was 2.96 ± 1.18 kg CO₂ eq per kg FPCM, but farm rankings changed when impacts were expressed per hectare. Pearson correlation and linear regression analyses showed a significant positive relationship between income and greenhouse gas emissions (r = 0.80, p < 0.05), indicating a trade-off between economic and environmental performance. Enteric methane and energy use were the main contributors to climate impacts. Improving productivity per animal rather than expanding herd size appears to be the most promising strategy to enhance profitability while limiting environmental burdens. Full article

Maintaining udder health and milk quality is important in dairy goat production; however, the interpretation of somatic cell count (SCC) in goats is complicated by physiological and non-infectious factors. This study evaluated the associations among udder and teat morphological traits, SCC, bacteriological status, and udder surface temperature in clinically healthy Saanen dairy goats. Udder conformation was assessed using a linear scoring system in multiparous goats (n = 70). Composite milk samples were collected at three lactation stages for SCC and milk quality analysis, whereas separate half-udder milk samples were used for bacteriological examination. Udder surface temperature was measured using infrared thermography (IRT) at the final sampling time point. Stepwise multiple regression analysis was applied to identify udder and teat traits associated with log-transformed SCC. The results showed considerable variation in udder conformation, with the greatest deviations observed for teat placement. Fore udder attachment, udder cleft, teat length, and teat form were significantly associated with SCC, with stronger attachment, a more distinct udder cleft, shorter teats, and a more cylindrical teat shape being associated with lower SCC. Bacteriological examination detected mastitis-associated bacteria in 45.0% of half-udder samples, mainly coagulase-negative Staphylococcus spp. and Corynebacterium spp., while the proportion of obligate udder pathogens was highest in the >2,000,000 cells/mL SCC category. Bacteriologically positive udder halves showed slightly higher surface temperatures than bacteriologically negative udder halves (p < 0.05), whereas no significant differences in udder surface temperature were observed among SCC categories. These findings suggest that udder morphology is associated with SCC and may be useful in phenotypic udder health assessment. IRT may provide complementary information on bacteriological status under standardized conditions, but its diagnostic value in dairy goats requires further validation in larger, longitudinal studies. Full article

Trypanosomiasis remains a major constraint to small ruminant production in sub-Saharan Africa, particularly among smallholder farmers. Although WAD goats are considered trypanotolerant because of their relatively low mortality during chronic infection, recent findings show that this survival is at the expense of reproductive efficiency. To back up this claim with scientific evidence, this review followed PRISMA guidelines and systematically searched PubMed, Scopus, and Web of Science for important studies published between January 1980 and February 2026. Search terms included African animal trypanosomiasis, Trypanosoma spp., WAD goats, reproductive dysfunction, trypanotolerance, oxidative stress, and hypothalamic–pituitary–gonadal axis. Of the 1245 retrieved articles, 14 met the inclusion criteria. Evidence from the included studies indicates that chronic trypanosome infection disrupts reproduction through interconnected mechanisms involving systemic inflammation, oxidative stress, endocrine imbalance, and impaired gonadal function. Available evidence suggests that T. brucei is frequently associated with ovarian dysfunction and embryonic loss, whereas T. congolense has been linked in some studies to uterine pathology and gestational reproductive disturbances. Female goats commonly exhibit irregular oestrous cycles, embryonic loss, and prolonged kidding intervals, while males develop impaired spermatogenesis, abnormal sperm morphology, and reduced testosterone levels. These reproductive impairments reduce kid output, milk yield, herd productivity, and household livelihood resilience. Integrated control strategies combining vector control, targeted chemotherapy, nutritional support, and selective breeding are essential for preserving both fertility and survival in trypanosome-endemic areas. Full article

Phytoestrogens are plant-derived phenolic compounds that structurally resemble endogenous estrogens and can exert both estrogenic and anti-estrogenic effects in animals. In ruminant nutrition, the main classes of phytoestrogens (isoflavones, lignans, stilbenes, coumestans and selected flavonoids) are supplied predominantly by legume forages and soybean-based feeds, in which concentrations can reach several mg/g of dry matter. After ingestion, these compounds are extensively metabolized by the rumen microbiota to derivatives with altered biological potency, such as equol and p-ethyl-phenol, which influence endocrine, immune and metabolic pathways. Experimental and field studies in cattle, sheep and goats indicate that dietary phytoestrogens may improve nitrogen utilization, immune competence, growth performance, antioxidant status and milk yield. However, they can also impair fertility, modify hormone profiles and compromise embryo survival in a compound-, dose-, and species-dependent manner. In this review, we summarize current knowledge on the botanical and nutritional sources, ruminal metabolism and transfer of phytoestrogens in ruminants, and critically examine their effects on blood metabolites, immune responses, growth and carcass traits and lactational performance and reproductive function. A structured literature search based on PRISMA principles was used to identify and appraise experimental and observational studies in both grazing and intensive production systems up to 2025. Remaining knowledge gaps and practical implications for the safe use of phytoestrogen-rich feeds in livestock production are highlighted. Full article

Milk adulteration is a common form of food fraud, particularly in high-value dairy products from small ruminants. A frequent practice involves dilution with water, often combined with the addition of sugars to mask physicochemical changes and avoid detection during routine quality control. This study aimed to develop an analytical approach for detecting combined adulteration in goat and sheep milk involving both water dilution and sucrose addition. Controlled experiments were conducted by diluting milk samples with water (1–15%) followed by the addition of sucrose solutions. Changes in physicochemical parameters, including fat, protein, total solids, lactose, density, freezing point depression, mineral content, and pH, were evaluated using an automated milk analyzer. In parallel, a suspected adulterant powder was characterized using conventional chemical analysis, ICP-AES, and HPLC-RI, revealing a composition predominantly of sucrose (91.4% w/w) with elevated sodium levels. Sucrose in milk samples was subsequently quantified using an enzymatic spectrophotometric method. Water dilution reduced protein, total solids, and density, while sucrose addition partially restored these parameters, masking adulteration effects. However, sucrose was reliably detected at concentrations above 0.1%. The proposed workflow may provide a practical and cost-effective complementary tool for routine dairy authenticity surveillance and fraud prevention. Full article

Foot-and-Mouth Disease (FMD) is caused by the FMD virus. Indirect Sandwich Enzyme-Linked Immunosorbent Assay (IS-ELISA) was standardized to characterize the FMD serotype “O” virus. Total protein content in the guinea pig serum (whole serum), ammonium sulfate precipitated guinea pig serum (ASPGPS) protein and ion-exchange-based purified guinea pig serum (IEGPS) protein was measured as 52 µg/mL, 24 µg/mL and 10 µg/mL respectively. The whole serum of guinea pigs and rabbits showed the 1:32 and 1:64 anti-FMD serotype “O” virus neutralizing antibody titers, while the anti-FMD serotype “O” virus neutralizing antibody titer was 1:128 in the IEGPS proteins. IEGPS protein with 1:128 neutralizing antibody titers were used as capture/trapping antibodies in the standardization of the assay. The IEGPS protein 1:1000 diluted with 10 µg/mL of protein content was found to be optimum for capture/trapping antibodies. To cover residual blank spaces, different available blocking buffers were evaluated and Skimmed Milk Solution 5% in Phosphate-Buffered Saline (PBS5%) proved best amongst blocking buffers. Coating of 1:1000 diluted IEGPS at 37 °C for 1 h followed by storage at 4 °C for overnight was best for incubation time. FMD serotype “O” virus 1:100 diluted was optimum in IS-ELISA. Similarly rabbit anti-FMD serotype “O” virus specific immune serum 1:10,000 diluted and goat anti-rabbit IgG horseradish peroxidase conjugate 1:4000 diluted were found to be optimum during the standardization of the assay. Lastly ELISA plates proved to be best amongst the available plates for assay. In each experiment, the plateau region, test background and plate background were recorded. Lastly it became possible for the establishment of an optimized and potentially cost-effective IS-ELISA requiring further diagnostic validation in research and diagnostic laboratories in the country. Full article

This study evaluated the effects of replacing soybean meal with cashew nut roasting residue (CNRR) on feed intake, nutrient digestibility, rumen fermentation, blood metabolites, and economic efficiency in lactating crossbred Saanen goats. Fifteen does were assigned to a completely randomized design with three treatments: 0% CNRR (T1), 10% replacement of soybean meal protein (T2), and 20% replacement (T3). Goats were fed pangola hay ad libitum and concentrate according to milk yield (1:2). Total dry matter, organic matter, crude protein, and fiber intake were not affected by dietary treatments (p > 0.05). However, crude protein digestibility decreased from 68.83% in the control group to 52.72% and 51.21% in the CNRR treatments (p < 0.05). Rumen pH and total volatile fatty acids remained within normal physiological ranges, suggesting stable rumen fermentation. Blood parameters, including packed cell volume, glucose, and blood urea nitrogen, were not influenced by CNRR supplementation (p > 0.05). Economic analysis showed reduced feed cost with increasing CNRR inclusion. In conclusion, CNRR can replace soybean meal protein at rates of up to 20% without affecting feed intake, ruminal pH, or measured blood metabolites; however, crude protein digestibility was reduced, indicating that dietary formulation may require adjustment. Full article

This study investigated how Pb, Cd, and Cu are distributed between curd and whey during milk coagulation in milk from different animal species, and how the level of metal addition and the coagulation method influence metal retention. Raw milk from buffalo, cow, donkey, goat, and sheep was supplemented with Pb, Cd, and Cu under controlled laboratory conditions at two levels corresponding to the regulatory maximum level (ML) and ten times this level (10 × ML). All three metals were added simultaneously to the same milk aliquot, and coagulation was induced either enzymatically or by acidification at pH 4.6. Metal concentrations in curd and whey were determined by atomic absorption spectrophotometry. In all milk types, Pb, Cd, and Cu were retained mainly in the curd fraction. At ML, curd retention generally ranged from about 77% to 97%, whereas at 10 × ML, retention decreased and transfer to whey increased. Donkey milk consistently showed lower metal retention in curd than ruminant milk. Statistical analysis of curd retention showed that metal type, milk species, the level of metal addition, and their interactions significantly influenced metal retention, indicating that the effect of coagulation method depended on the experimental conditions rather than being uniform across all cases. Overall, the results show that milk coagulation favours the association of Pb, Cd, and Cu with the curd fraction, highlighting the importance of the milk protein phase in determining metal distribution during dairy processing. These findings improve our understanding of heavy-metal behaviour during milk processing and help clarify their potential transfer into curd-based dairy products. Full article

Background/Objectives: Gastroesophageal reflux disease (GERD) is prevalent in infants and frequently managed with acid-suppressive medications that elevate gastric pH. This pilot study aimed to evaluate how varying gastric pH levels (2.5, 4.0 and 6.0) influence the hydrolysis of milk proteins in human milk (HM), cow’s milk-based infant formula (CMF), and goat milk-based infant formula (GMF). Methods: Samples were subjected to a 30 min in vitro gastric digestion using pediatric human gastric juice obtained from clinical donors. Protein degradation was analyzed via SDS-PAGE densitometry, comparing digested aliquots to undigested controls. Results: At pH 2.5, caseins were highly digested in all samples, especially in HM and GMF. At pH 4.0, GMF displayed an apparent 51% greater casein degradation relative to CMF and HM in this pilot analysis. α-lactalbumin degradation was markedly higher in GMF at all pH levels; notably, at pH 4.0 and 6.0, only GMF exhibited digestion of this protein. Albumin showed almost complete degradation in HM and GMF at pH 2.5, and GMF maintained greater degradation at higher pH levels. β-lactoglobulin (absent in HM) was better digested in GMF at pH 2.5, whereas CMF showed higher hydrolysis observed at pH 4.0 and 6.0. Lactoferrin digestion was most efficient in HM and GMF at pH 2.5, with no differences observed at higher pH levels. Conclusions: These preliminary findings suggest that GMF may offer digestive advantages for infants with GERD under pharmacological acid suppression, particularly regarding casein and α-lactalbumin breakdown at higher pH. The distinct digestion kinetics of CMF and GMF at different pH levels provide a physiological basis for targeted infant feeding strategies. Further large-scale studies are required to validate these exploratory observations. Full article

Despite serological evidence of tick-borne encephalitis virus (TBEV) circulation in Belgian animals since 2007, confirmed autochthonous human infection was only first documented in 2020. We review the current national epidemiologic situation and investigate a household cluster of confirmed autochthonous cases identified in 2025. A cohabiting couple experienced a near-simultaneous onset of meningoencephalitis and tested positive for TBEV-specific IgM and IgG, with confirmation by PRNT90. One patient reported a recent tick bite, and both patients reported consumption of unpasteurized milk and goat cheese, suggesting possible alimentary transmission. The identification of Case 2, who lacked neurological symptoms at presentation and was only tested due to the index case, illustrates the risk of missed diagnoses and supports the notion that human TBEV infection is likely underdiagnosed in Belgium. These findings underscore the need to increase clinical awareness, strengthen surveillance, and reinforce prevention strategies. TBE should be considered in the differential diagnosis of patients presenting with non-specific fever or neurological syndromes such as meningoencephalitis, particularly during the spring-to-autumn tick activity season. Full article

The Comarca Lagunera region faces groundwater quality issues due to elevated concentrations of heavy metals that exceed permissible limits set by Mexican regulations. This study aimed to evaluate arsenic concentrations in drinking water and goat milk, as well as their possible association with DNA fragmentation in goat lymphocytes in the Comarca Lagunera (Durango and Coahuila, Northern Mexico). Water, milk, and blood samples were collected from 120 goats (Capra hircus) and analyzed using atomic absorption spectrophotometry (water and milk samples) and the comet assay (blood). Arsenic concentration in drinking water varied among locations, with the highest value detected in El Venado (San Pedro, Coahuila), while other sites showed concentrations close to permissible limits. Arsenic concentrations in goat milk were generally low and mostly below the LOQ, which limited the ability to assess arsenic transfer into milk. DNA fragmentation was observed in lymphocytes; however, no statistically significant association was found between arsenic concentrations and DNA damage. These results indicate that, under the conditions of this study, DNA damage cannot be directly attributed to arsenic exposure and may be influenced by other environmental or biological factors. Further studies with larger sample sizes and additional variables are recommended. Full article

This study investigated the microbiological composition and antimicrobial resistance (AMR) profiles of artisanal goat and sheep milk cheeses produced in Poland. Ten raw milk cheeses (five each from goat and sheep milk) were analyzed using a combined approach involving culture-dependent enumeration, 16S rRNA gene sequencing, and antibiotic susceptibility testing. Microbial counts revealed substantial variability among the samples, with lactic acid bacteria (LAB) dominating the microbiota. Taxonomic analysis confirmed the predominance of Lactococcus, Streptococcus, and lactobacilli, although marked intra-group heterogeneity was observed. Multivariate analyses indicated that sample-specific factors had a greater influence on microbiome composition than milk origin. Among 170 isolates, 28.7% were classified as multidrug-resistant (MDR), being most prevalent in Enterobacterales (100%) and Enterococcus spp. (73%), whereas LAB exhibited low resistance levels (16.2%). Resistance was most frequently associated with aminoglycosides and β-lactams. The resistance results were interpreted according to CLSI guidelines. These findings demonstrate that artisanal cheeses harbor complex, dynamic microbial ecosystems that may serve as reservoirs of antimicrobial resistance. The results highlight that environmental and technological factors, rather than milk source alone, are key drivers of both microbiome structure and resistance distribution, underscoring the need for targeted AMR monitoring in traditional dairy products. Full article

Sheep and goat milk are mainly used for cheese manufacture in small livestock farms, giving rise to a large volume of whey. Sheep and goat cheese whey possess excellent and specific functional and nutritional characteristics. The valorization of these valuable by-products through physicochemical or biotechnological processes compatible with artisanal production are important in terms of sustainability, i.e., economic, social, and environmental impacts. The main challenges for whey processing in small and medium-size enterprises (SMEs) are the lack of equipment, construction and information as well as the small amounts of cheese whey generated from these plants. Membrane technology can be convenient to produce valuable by-products for small dairy plants in the presence of enough investment cost and whey amount. Biotechnological treatments covering anaerobic digestion systems and fermentation processes are advantageous for SMEs over physicochemical methods on investment cost. In these processes, efficient microorganisms are able to produce high-value natural products, biofuels, and biopolymers. Anaerobic digestion is a suitable method for goat and sheep cheese whey valorization in SMEs due to the small volumes. Additionally, bioconversion into fermented beverages is a good choice for cheese whey valorization in SMEs because of its low operational and equipment cost. Full article