Search Results (1,399)

Gluten-free fermented products remain technologically challenging due to the absence of gluten, which plays a key role in stabilizing batter structure and gas retention. This study proposes a mixture design-driven approach to develop gluten-free Algerian pancakes based on rice and teff formulations enriched with legumes and seeds, aiming to restore techno-functional properties while improving nutritional quality. Two formulations, a teff-based (TBF) and a rice-based (RBF) system, were optimized using a simplex centroid mixture design and evaluated in comparison with durum wheat pancakes. The results demonstrated that formulation strongly influenced batter rheology and final structure. The TBF system exhibited superior technological performance, with higher specific volume (1.77 cm³/g), lower density (0.56 g/cm³), and enhanced porosity, associated with improved protein and fiber content. In contrast, the RBF formulation showed higher antioxidant activity. The findings highlight the critical role of component interactions in modulating batter viscosity and foam stability, which directly affected pore development and product airiness. Both optimized formulations successfully reproduced the characteristic “light and airy” structure of traditional pancakes, achieving good sensory acceptability. Overall, this study demonstrates that mixture design can effectively guide the development of gluten-free fermented systems by linking composition, rheology, and structural properties, providing a strategy for improving the quality of traditional gluten-free foods. Full article

Objectives: To evaluate serum zonulin and CHI3L1 as indicators of intestinal permeability and disease activity in pediatric celiac disease and to explore their associations with histopathological findings and nutritional status. Methods: This prospective cross-sectional study included 131 pediatric patients with CD (aged 2–18 years) and 42 healthy controls. Patients were classified as newly diagnosed, gluten-free diet (GFD)-adherent, or GFD-nonadherent. Body mass index was calculated, and serum levels of micronutrients, zonulin, and CHI3L1 were measured using a sandwich enzyme-linked immunosorbent assay. Associations with histopathological findings, serological markers, and nutritional parameters were analyzed. Results: Age and sex distributions were similar across groups (mean age: 10.9 ± 4.27 years). Serum zonulin and CHI3L1 levels were moderately positively correlated (r = 0.525, p < 0.001). Both biomarkers showed significant positive correlations with Marsh scores and tissue transglutaminase IgA levels. Zonulin was inversely correlated with hemoglobin, serum iron, and ferritin, whereas CHI3L1 showed negative correlations with hemoglobin and folate. Parathyroid hormone levels were positively correlated with both biomarkers. Receiver operating characteristic analysis demonstrated acceptable discriminatory performance for distinguishing CD from controls (AUC: 0.713 for zonulin and 0.709 for CHI3L1). Conclusions: Serum zonulin and CHI3L1 levels are associated with disease activity and mucosal injury in pediatric CD but do not directly reflect micronutrient status. These biomarkers may complement conventional monitoring parameters by providing additional information on intestinal permeability and inflammatory activity during follow-up. Full article

Background: Celiac disease (CeD) requires lifelong adherence to a gluten-free diet (GFD). However, there is evidence that a GFD may lead to an unhealthy cardiometabolic risk profile and potentially increase the risk of cardiovascular disease in some patients. Incorporating plant-based foods (primarily derived from plants) into a GFD may offer a solution to improve cardiometabolic health. Thus, this study aimed to identify the attitudes toward and barriers to adopting a more plant-dominant diet among Danish patients with CeD. Methods: A cross-sectional survey was distributed to 2861 members of the Danish Celiac Society. Data from 959 patients with confirmed CeD were included. Results: Most participants (58.5%) reported adapting their diet after diagnosis by combining gluten-free products with plant-based foods, while 31.2% relied solely on gluten-free replacements. Dietary adaptation was primarily shaped by the limited availability of gluten-free plant-based foods (64%), taste/texture (55%), and cost (51%). More than half of the patients (56.8%) considered ‘eating more plant-based foods’, with ‘health’ being the primary motivator (70%), followed by ‘climate’ (50%) and ‘taste’ (36%). However, several barriers to a more plant-dominant diet were identified. Most notably, ‘taste and texture’ (71%), ‘limited availability of gluten-free plant-based foods’ (68%), ‘nutritional concerns’ (56%), and ‘cost’ (54%) were reported as barriers. Conclusions: Most Danish patients with CeD were generally positive about increasing their intake of plant-based foods; however, barriers to such dietary changes remain. Ongoing follow-up, practical guidance from dietitians, and accessible evidence-based resources may help patients maintain a nutritionally balanced, plant-dominant GFD that supports long-term health. Full article

Background/Objectives: Celiac disease is a chronic autoimmune disorder triggered by gluten ingestion in genetically predisposed individuals. In children and adolescents, it presents heterogeneously and may negatively affect physical, psychological, and social well-being. Although a strict gluten-free diet is the only effective treatment, it may also impose important dietary, social, and economic burdens. This systematic review aimed to evaluate the impact of celiac disease on the quality of life of affected children and adolescents and their families. Methods: This systematic review was conducted according to PRISMA, AMSTAR 2, and Cochrane Handbook recommendations. Searches were performed in PubMed, Scopus, CINAHL, Web of Science, and PsycINFO for studies published between 2019 and 2026 in English or Spanish. Quantitative, qualitative, and mixed-methods studies on pediatric celiac disease and quality of life were included. Two reviewers independently conducted study selection, data extraction, and risk-of-bias assessment. Due to study heterogeneity, a narrative synthesis was performed. Results: Thirteen studies were included. Children and adolescents with celiac disease generally reported lower quality of life, particularly in emotional, social, and school-related domains. Adherence to a gluten-free diet was an important factor associated with quality of life. Although it improved symptoms, it was also linked to social restrictions, nutritional imbalances, and financial burden. Families also reported stress, lifestyle changes, and reduced well-being. Findings should be interpreted cautiously due to heterogeneity and variability in methodological quality across studies. Conclusions: Current evidence suggests that celiac disease may have a multidimensional impact on the quality of life of pediatric patients and their families. These findings support the need for a comprehensive multidisciplinary approach addressing dietary, psychosocial, and family-related factors. Full article

Objective: This scoping review aimed to map the methodological approaches used to assess disordered eating attitudes and behaviors in children and adolescents with celiac disease (CD). Methods: This review followed the Joanna Briggs Institute scoping review methodology and the PRISMA-ScR guidelines, including studies of children and adolescents with CD that used methodological approaches to assess disordered eating attitudes and behaviors in the context of the gluten-free diet (GFD). No restrictions were applied regarding geography, language, or year. Searches were conducted across 10 electronic databases. Data were descriptively analyzed and presented in tables or diagrams, with a narrative synthesis aligned to the review objective. Results: Studies from 13 countries were included and classified as quantitative (n = 16; 51.6%), qualitative (n = 11; 35.5%), or mixed-methods (n = 4; 12.9%). A total of 34 instruments were used, but only one was specifically designed to evaluate eating attitudes and behaviors in children and adolescents with CD. Conclusions: Analysis of disordered eating in children and adolescents with CD remains methodologically heterogeneous and evolving. Quantitative studies predominate but often rely on non-specific instruments that blur the distinction between adaptive dietary vigilance and disordered eating. Qualitative and mixed-methods approaches highlight lived experiences and reveal measurement gaps despite their higher costs. Progress depends on developing specific instruments for this population that better capture the complexity of GFD management across development. Full article

Celiac disease (CD) is a chronic immune-mediated enteropathy triggered by the ingestion of gluten in genetically predisposed individuals. While the adaptive immune response to deamidated gliadin peptides represents a central pathogenic mechanism, growing evidence suggests that epithelial stress and innate immune activation play a fundamental role in the onset and persistence of the disease. Heat shock proteins (Hsps), central regulators of cellular proteostasis, have emerged as potential mediators at the interface between epithelial distress and immune signaling. This review discusses the involvement of major Hsp families, including Hsp27, Hsp60, Hsp70, and Hsp90, in the pathophysiology of CD. The altered expression of Hsp27 and Hsp70 in the intestinal mucosa reflects a persistent state of epithelial stress that often persists despite a strict gluten-free diet (GFD). We focus specifically on Hsp60, whose extracellular release under stress conditions may allow it to function as a damage-associated molecular pattern (DAMP), engaging Toll-like receptors and promoting NF-κB- and inflammasome-dependent inflammatory pathways. Although direct mechanistic evidence linking Hsp60 to CD remains limited, the convergence of epithelial stress signs, Toll-like receptor (TLR) upregulation, and prolonged innate immune activation supports the hypothesis of a stress-induced inflammatory amplification circuit in the coeliac mucosa. Further studies are essential to clarify the pathogenic relevance and potential therapeutic implications of this proposed axis. Full article

Evidence suggests that a gluten-free diet may increase the risk of metabolic abnormalities associated with cardiovascular disease in adults with Coeliac Disease (CeD). This 9-week, double-blind, placebo-controlled, randomised pilot study investigated the effects of a combined supplement containing probiotic Lactiplantibacillus plantarum ECGC 13110402 and plant sterols and stanols, on cardiometabolic biomarkers and gut microbiota diversity and composition in adults with CeD and hypercholesterolaemia. Blood lipid profiles and vitamin D concentrations were analysed, and gut microbiota was profiled via 16S rRNA amplicon sequencing. In the active group, significant reductions in total cholesterol, LDL-cholesterol, non-HDL cholesterol, and apolipoprotein B were observed at multiple time points during the treatment phase, with changes generally greater in magnitude compared with the placebo group. Vitamin D levels also increased in the active group during supplementation. Microbiota analysis revealed potentially beneficial changes in participants receiving the active formulation, including higher alpha diversity and higher proportions of Bifidobacterium spp., Christensenellaceae R-7 group, and Lachnospiraceae ND3007 group. Overall, this feasibility study provides exploratory findings that a combined Lactiplantibacillus plantarum ECGC 13110402-phytosterol formulation may support lipid management and beneficially modulate gut microbiota in adults with CeD, particularly for those seeking non-pharmacological approaches to improving cardiometabolic health biomarkers. Full article

Background/Objectives: Avoidant/restrictive food intake disorder (ARFID), a non-body image-based eating disorder, has been described in several chronic gastrointestinal diseases, but little is known about ARFID in celiac disease (CeD) and non-celiac gluten sensitivity (NCGS). We aimed to identify the prevalence and characteristics of ARFID among adult and pediatric patients presenting for consultation of gluten-related disorders longitudinally. Methods: We reviewed 386 consecutive referrals (ages 2–82 y; 68% female) to a tertiary care celiac center from June 2019 to June 2022. Eligible patients had histologically confirmed CeD or were classified as NCGS based on gluten-related symptoms but negative serologies and histology. Masked coders applied DSM-5 criteria for ARFID at initial presentation and one-year follow-up visit. Results: Out of 386 patients, 253 had biopsy information and were included in the study. ARFID symptoms were present in 19% (47/253) of patients at consultation and in 17% (22/126) at follow-up. ARFID symptoms were equally present among patients with CeD and NCGS and were not associated with the presence or duration of a gluten-free diet, but patients with ARFID were more likely to be on a non-gluten-free diet at presentation. In multivariate analysis, the likelihood of having ARFID symptoms at consultation increased with a history of weight loss (OR 2.87, 95% CI 1.36–6.30). Conclusions: We found that almost one-fifth of patients with CeD and NCGS had symptoms of ARFID at consultation. At follow-up, among a smaller cohort, ARFID prevalence remained similar, although some patients no longer had ARFID symptoms, and some had new ARFID symptoms develop. Further longitudinal research is needed to understand the risk and maintenance factors of ARFID in the context of CeD and NCGS. Full article

Proso millet (Panicum miliaceum L.) is being increasingly used in gluten-free baking; however, the influence of cultivar-dependent functionality on gluten-free dough remains insufficiently characterized. This study systematically evaluated the impact of nine proso millet cultivars (Cope, Dawn, Sunrise, Earlybird, Huntsman, Minco, Panhandle, Plateau, and Rise) on dough rheology, bread quality, and texture stability in a gluten-free formulation. Dough viscoelasticity was characterized using small-amplitude oscillatory shear (G′, G″, tan δ) and creep–recovery (J_end, J_nr, J_r/J, strain recovery, and t₉₀). Breads were then evaluated for specific volume, crust and crumb color, and texture profile analysis (TPA) over 0, 2, and 5 days of storage. All doughs exhibited weak gel behavior (tan δ = 0.30–0.36) with G′ consistently exceeding G″. The waxy, low-amylose cultivar Plateau produced the stiffest dough (highest G′ and G″) and the lowest loaf specific volume (1.97 mL/g), whereas Rise and Earlybird yielded the greatest expansion (2.43–2.40 mL/g). Storage induced typical staling (increased firmness, decreased springiness, cohesiveness, and resilience) with cultivar-dependent retention of elastic attributes linked to rheological parameters. Overall, cultivar starch structure impacts dough viscoelasticity, loaf expansion, and texture evolution, highlighting cultivar selection as a practical route to improve gluten-free bread quality and shelf-life consistency. Full article

Acorns (Quercus spp.) have a long history of use in human nutrition and represent an underutilized plant resource with considerable potential for sustainable food systems. In recent years, acorn flour (AF) has attracted scientific interest as an innovative ingredient, particularly in breadmaking, due to its lack of gluten, favorable nutritional profile, and technological functionality. This review summarizes bread made with AF as a sustainable and innovative food product, with particular emphasis on its technological properties, including rheological behavior, sensory attributes, and nutritional profile. To our knowledge, this review is among the first to integrate an analysis of recent technological advancements in breadmaking with the current European Union regulatory framework, specifically EU Organic Regulation 2018/848. By bridging the gap between food technology and legislative requirements, the review outlines a comprehensive pathway for the commercialization of acorns as a certified organic resource. It concludes that although acorns have significant potential to enhance food security and sustainability, further standardization and increased consumer awareness are necessary to ensure their successful market integration. Full article

The development of gluten-free biscuits with high nutritional value presents a challenge for the food industry. This study evaluated the dough behavior and quality characteristics of gluten-free biscuits obtained using the raw materials sorghum flour, sesame paste, and Moringa oleifera leaf powder. Ten formulations were developed, including a control sample without moringa, using a mixture design that generated different combinations between sorghum flour, sesame paste, and powdered moringa. Moringa-enriched biscuits showed significant nutritional improvements, with protein increasing by 40% (12.07–16.93%), fiber by 92% (2.78–5.34%), polyphenols more than twofold (52.88–120.66 mg GAE/100 g), and flavonoids more than threefold (110.44–335.30 mg QE/100 20 g). Technological properties such as rheology, texture, color, and water activity varied with formulation. Moringa addition darkened biscuits (L* 35.61–49.74) and increased hardness by 62% (20.53–33.19 N). All doughs exhibited dominant viscoelastic behavior (G′ > G″), with higher sorghum levels leading to increased viscoelasticity. FTIR analysis confirmed characteristic functional groups of carbohydrates, lipids, and proteins across samples. Sensory evaluation indicated good overall acceptance, with a preference for the control sample and the sorghum-rich formulation (F4), which contained the lowest amounts of sesame paste and powdered moringa. Overall, Moringa oleifera enhances both nutritional and technological properties of gluten-free biscuits; therefore, it can contribute to the development of functional products from local resources. Full article

Background/Objectives: Celiac disease (CD) is a chronic immune-mediated enteropathy triggered by gluten ingestion in genetically predisposed individuals, with a highly heterogeneous clinical presentation encompassing both intestinal and extraintestinal manifestations. Despite growing clinical awareness, diagnostic delay remains a significant challenge, and the evolution of clinical and histopathological features following gluten-free diet (GFD) initiation remains incompletely characterized. This study aimed to compare the clinical, serological, and histopathological profile of adults with newly diagnosed CD and those maintained on a long-term GFD. Methods: This prospective observational study enrolled 50 adult patients with biopsy-confirmed CD: 16 at the time of diagnosis (gluten-consuming) and 34 on a GFD for at least one year. All participants underwent standardized clinical assessment, serological testing (IgA anti-tissue transglutaminase [tTG] and/or IgA anti-endomysial antibodies [EMAs]), upper digestive endoscopy with duodenal biopsies graded according to the Marsh–Oberhuber classification, and evaluation for Helicobacter pylori co-infection. Between-group comparisons were performed using the Mann–Whitney U test, Fisher’s exact test, and chi-square test, as appropriate. Results: Newly diagnosed patients exhibited a significantly longer median duration from symptom onset to diagnosis compared to the GFD group (p = 0.03). Histopathological severity was greater in the newly diagnosed group, with more advanced Marsh–Oberhuber grades (p = 0.03). Among individual symptoms, bloating was significantly more frequent in newly diagnosed patients (p = 0.02). Notably, thrombocytosis was identified significantly more often in the newly diagnosed group compared to GFD patients (p = 0.02), representing a potentially underrecognized extraintestinal marker of active CD. Overall rates of intestinal and extraintestinal manifestations and CD-specific seropositivity did not differ significantly between groups. Conclusions: The clinical and histopathological profile of adults with celiac disease differs meaningfully between the time of diagnosis and during GFD adherence. Bloating and thrombocytosis were significantly more prevalent at diagnosis, with thrombocytosis emerging as a potentially underrecognized marker of active disease. Conversely, several manifestations persisted despite dietary treatment, underscoring the heterogeneous nature of CD across its clinical course. These findings may support earlier disease recognition and more individualized follow-up strategies in routine clinical practice. Full article

Cañihua (Chenopodium pallidicaule) is an underutilized Andean pseudocereal of strategic interest for sustainable agriculture in high-altitude, climate-constrained environments, where its tolerance to frost, drought, and saline soils positions it as a potential climate-resilient crop. Despite its high nutritional value and potential for functional food applications, its research landscape remains fragmented and unevenly developed across agronomic, nutritional, and technological dimensions. This study aimed to systematically and bibliometrically analyze the scientific literature on cañihua published between 1995 and 2025. A total of 104 documents indexed in the Scopus database were evaluated following the PRISMA 2020 approach, including analyses of publication trends, geographic distribution, collaboration networks, and thematic structures, together with a qualitative critical appraisal of the included evidence. Results indicate a marked increase in scientific output since 2006, with research predominantly concentrated in food science and technology and limited development in agronomy, clinical nutrition, and socio-economic domains. Thematic analysis reveals a strong focus on bioactive compounds, nutritional composition, and processing technologies, while clinical, socio-economic, and large-scale agricultural studies remain limited. Processing strategies such as germination, malting, and fermentation enhance nutrient bioavailability, reduce antinutritional factors, and improve sensory properties, supporting the incorporation of cañihua into functional and gluten-free foods at levels of up to 25%. Significant gaps persist in clinical validation, agronomic standardization, production scalability, genetic improvement, and integration across research domains. Overall, cañihua shows strong potential to contribute to sustainable Andean agriculture, food security, and functional food innovation, although further interdisciplinary and translational research linking agricultural production with nutritional and technological outcomes is required to realize its full applied potential. Full article

Gluten-free cakes formulated solely with rice flour frequently exhibit limited volume, weak internal structures, and rapid quality deterioration. This study investigated the effects of replacing rice flour with 0, 3, 6, 9, and 12% goji berry powder (GBP) in gluten-free cupcakes. Physical properties, texture profile, crumb porosity, crust and crumb color, flour pasting behavior, and antioxidant properties were evaluated. Moderate GBP addition improved cupcake quality, with the 6% treatment showing the greatest height (45.17 mm) and specific volume (3.64 cm³/g), the lowest hardness (327.50 g), the highest springiness (9.25 mm), and the largest average pore area (0.42 mm²). In contrast, higher substitution levels (9–12%) increased moisture and reduced baking loss but caused a decline in specific volume and a marked increase in hardness. GBP progressively darkened the product, especially the crumb, while significantly enhancing total phenolic content and DPPH radical-scavenging activity from 55.46 to 67.36 mg gallic acid equivalents (GAE)/100 g and from 4.85 to 15.08 mg ascorbic acid equivalents (AAE)/100 g, respectively. Monotonic decreases in peak, trough, final, and setback viscosities were observed, indicating reduced starch swelling and retrogradation tendencies as the GBP level increased. GBP at 6% showed the most balanced overall performance, while 12% maximized the antioxidant response at the expense of structural quality. Full article

Celiac disease (CD) is an autoimmune inflammatory enteropathy triggered by consuming gluten-containing cereals. A key role in its pathogenesis is played by type 2 transglutaminase, an enzyme that causes an increase in gluten immunogenicity. Celiac cells seem to present constitutive characteristics, even recognizable under a gluten-free diet, such as defects in vesicular trafficking and autophagy, protein hyperphosphorylation, and cytoskeleton rearrangement. In this work, by using an omics approach, we attempted to identify those proteins differentially expressed or differentially phosphorylated in a cell model suitable to study cell behavior in the absence of inflammation, i.e., primary cultures of dermal fibroblasts from control or CD subjects. By performing mass spectrometry analyses, we found several up- and-down expressed or phosphorylated proteins in CD samples, mainly involved in signaling, homeostatic responses, cytoskeleton organization, vesicular trafficking, and extracellular vesiculation. These proteins may represent a molecular signature of the celiac cellular phenotype and may contribute to adding new insight into the comprehension of the complex mechanisms of CD pathogenesis. Full article