Search Results (15)

Blockchain technology is being increasingly deployed to store and process transactions and information in the global financial sector. Blockchain underpins cryptocurrencies such as Bitcoin and facilitates decentralized finance (DeFi), representing a paradigm shift in the global financial landscape, offering alternative solutions to traditional banking, and fostering financial inclusion. In developing economies such as Morocco, where a significant portion of the population remains unbanked, these digital financial innovations present both opportunities and challenges. This study examines the potential role of cryptocurrencies and DeFi in enhancing financial inclusion in Morocco, where cryptocurrencies have been banned since 2017. However, the public continues to use cryptocurrencies, circumventing restrictions, and the Moroccan Central Bank is now preparing to introduce new regulations to legalize their use within the country. In this context, this article analyses the potential of cryptocurrencies to mitigate barriers such as high transaction costs, restricted access to financial services in rural areas, and limited financial literacy in the country. The study pursues a mixed-methods approach, which combines a quantitative survey with qualitative expert interviews and adapts the Unified Theory of Acceptance and Use of Technology (UTAUT) model to the Moroccan context. The findings reveal that while cryptocurrencies offer cost-efficient financial transactions and improved accessibility, their adoption may be constrained by regulatory uncertainty, security risks, and technological limitations. The novelty of the article thus lies in its focus on the key mechanisms that influence the adoption of cryptocurrencies and their potential impact in a specific national context. In so doing, the study highlights the need for a structured regulatory framework, investment in digital infrastructure, and targeted financial literacy initiatives to optimize the potential role of cryptocurrencies in progressing financial inclusion in Morocco. This underscores the need for integrated models and guidelines for policymakers, financial institutions, and technology providers to ensure the responsible introduction of cryptocurrencies in developing world environments. Full article

Achieving the Sustainable Development Goals (SDGs) is crucial for addressing global environmental challenges. SDG 13 calls for urgent climate action, while SDG 7 promotes sustainable energy. These objectives are particularly relevant to China, where transport-related CO₂ emissions continue to rise due to urbanization, industrial growth, and increasing energy demand. This study examines the impact of economic governance, population aging, human capital, financial innovation, GDP growth, and energy transition on China’s transport-related CO₂ emissions, using quarterly data from 2006Q1 to 2018Q4. The Method of Moments Quantile Regression (MMQR) is applied to analyze heterogeneous effects across different emission levels. The findings reveal that economic governance (ECOG), energy transition (ENT), and human capital (HI) significantly reduce transport CO₂ emissions (TCO_2E) by enhancing institutional effectiveness and promoting clean energy adoption. In contrast, population aging (POPAGE), financial innovation (FI), and GDP contribute to higher emissions by increasing energy consumption and private transport dependency. These insights highlight the need for stronger governance frameworks, sustainable financial policies, and increased investment in renewable energy. Policymakers should strengthen environmental regulations, expand green financing initiatives, and enhance public transport infrastructure to align with SDGs 7 and 13. By implementing these strategies, China can make significant progress toward reducing transport emissions, achieving carbon neutrality, and ensuring long-term sustainability. Full article

The tomato is a vital component of agriculture and is the second-most important vegetable globally. Maintaining a high tomato production requires both water quality and quantity. Water-scarce regions like Saudi Arabia still lack an understanding of the impact of deficit irrigation and the use of a blend of saline and freshwater, especially their nuanced impact across growth stages. The purpose of this study was to evaluate the effects of six different irrigation amounts: full irrigation with 100% ET_c (FI), regulated deficit irrigation with 60% ET_c (DI), and deficit irrigation with 60% ET_c, except for the initial (DI-int), development (DI-dev), mid-season (DI-mid), and late-season (DI-lat) stages. This was performed with three different water qualities: fresh (FW), saline (SW), and fresh-saline blend (1:1) (MW) water. FW and MW enhanced the growth, physiology, morphology, yield, and quality, while SW had the lowest values. DI reduced these parameters and lowered yields by 13.7%, significantly improving water use efficiency (WUE) by 44% and fruit quality. DI-mid or DI-lat slightly improved yields while remarkably decreasing WUE and fruit quality. DI outperforms deficit irrigation in all growth stages except one, and countries with limited freshwater resources can benefit from a mix of fresh and saline water with a 60% ET_c deficit irrigation, resulting in greater water savings. Full article

According to estimates by the Food and Agriculture Organization of the United Nations (FAO), about a third of all food produced for human consumption in the world is lost or wasted—approximately 1.3 billion tons. Among this, the amount lost during the storage stage is about 15–20% for vegetables and 10–15% for fruits. It is 5–10% for vegetables and fruits during the distribution stage, resulting in a large amount of resource waste and economic losses. At the same time, the global population affected by hunger has reached 828 million, exceeding one-tenth of the total global population. The improvement of the cold chain system will effectively reduce the amount of waste and loss of food during the storage and transportation stages. Firstly, this paper summarizes the concept and development status of traditional preservation technology; environmental parameter sensor components related to fruit and vegetable spoilage in the intelligent cold chain system; the data transmission and processing technology of the intelligent cold chain system, including wireless network communication technology (WI-FI) and cellular mobile communication; short-range communication technology, and the low-power, wide-area network (LPWAN). The smart cold chain system is regulated and optimized through the Internet of Things, blockchain, and digital twin technology to achieve the sustainable development of smart agriculture. The deep integration of artificial intelligence and traditional preservation technology provides new ideas and solutions for the problem of food waste in the world. However, the lack of general standards and the high cost of the intelligent cold chain system are obstacles to the development of the intelligent cold chain system. Governments and researchers at all levels should strive to highly integrate cold chain systems with artificial intelligence technology, establish relevant regulations and standards for cold chain technology, and actively promote development toward intelligence, standardization, and technology. Full article

Mitochondria, the membrane-bound cell organelles that supply most of the energy needed for cell function, are highly regulated, dynamic organelles bearing the ability to alter both form and functionality rapidly to maintain normal physiological events and challenge stress to the cell. This amazingly vibrant movement and distribution of mitochondria within cells is controlled by the highly coordinated interplay between mitochondrial dynamic processes and fission and fusion events, as well as mitochondrial quality-control processes, mainly mitochondrial autophagy (also known as mitophagy). Fusion connects and unites neighboring depolarized mitochondria to derive a healthy and distinct mitochondrion. In contrast, fission segregates damaged mitochondria from intact and healthy counterparts and is followed by selective clearance of the damaged mitochondria via mitochondrial specific autophagy, i.e., mitophagy. Hence, the mitochondrial processes encompass all coordinated events of fusion, fission, mitophagy, and biogenesis for sustaining mitochondrial homeostasis. Accumulated evidence strongly suggests that mitochondrial impairment has already emerged as a core player in the pathogenesis, progression, and development of various human diseases, including cardiovascular ailments, the leading causes of death globally, which take an estimated 17.9 million lives each year. The crucial factor governing the fission process is the recruitment of dynamin-related protein 1 (Drp1), a GTPase that regulates mitochondrial fission, from the cytosol to the outer mitochondrial membrane in a guanosine triphosphate (GTP)-dependent manner, where it is oligomerized and self-assembles into spiral structures. In this review, we first aim to describe the structural elements, functionality, and regulatory mechanisms of the key mitochondrial fission protein, Drp1, and other mitochondrial fission adaptor proteins, including mitochondrial fission 1 (Fis1), mitochondrial fission factor (Mff), mitochondrial dynamics 49 (Mid49), and mitochondrial dynamics 51 (Mid51). The core area of the review focuses on the recent advances in understanding the role of the Drp1-mediated mitochondrial fission adaptor protein interactome to unravel the missing links of mitochondrial fission events. Lastly, we discuss the promising mitochondria-targeted therapeutic approaches that involve fission, as well as current evidence on Drp1-mediated fission protein interactions and their critical roles in the pathogeneses of cardiovascular diseases (CVDs). Full article

Herein, we describe the global comparison of miRNAs in human pancreatic cancer tumors, adjacent normal tissue, and matched patient-derived xenograft models using microarray screening. RNA was extracted from seven tumor, five adjacent normal, and eight FI PDX tumor samples and analyzed by Affymetrix GeneChip miRNA 4.0 array. A transcriptome analysis console (TAC) was used to generate comparative lists of up- and downregulated miRNAs for the comparisons, tumor vs. normal and F1 PDX vs. tumor. Particular attention was paid to miRNAs that were changed in the same direction in both comparisons. We identified the involvement in pancreatic tumor tissue of several miRNAs, including miR4534, miR3154, and miR4742, not previously highlighted as being involved in this type of cancer. Investigation in the parallel mRNA and protein lists from the same samples allowed the elimination of proteins where altered expression correlated with corresponding mRNA levels and was thus less likely to be miRNA regulated. Using the remaining differential expression protein lists for proteins predicted to be targeted for differentially expressed miRNA on our list, we were able to tentatively ascribe specific protein changes to individual miRNA. Particularly interesting target proteins for miRs 615-3p, 2467-3p, 4742-5p, 509-5p, and 605-3p were identified. Prominent among the protein targets are enzymes involved in aldehyde metabolism and membrane transport and trafficking. These results may help to uncover vulnerabilities that could enable novel approaches to treating pancreatic cancer. Full article

A recent study has shown that DNA hypermethylation can promote ischemia reperfusion (I/R) injury by regulating the mitochondrial function. Diabetes mellitus (DM) is reported to induce DNA hypermethylation, but whether this prior DNA methylation in DM I/R heart inflicts a beneficial or detrimental effect is not known and is addressed in this study. DM was induced in 6-week-old male Wistar rats with streptozotocin (65 mg/kg b.wt). After 24 weeks on a normal diet, I/R was induced in rat heart using a Langendorff perfusion system and analyzed the myocardium for different parameters to measure hemodynamics, infarct size, DNA methylation and mitochondrial function. Diabetic heart exhibited DNA hypermethylation of 39% compared to the control, along with DNMT expression elevated by 41%. I/R induction in diabetic heart promoted further DNA hypermethylation (24%) with aggravated infarct size (21%) and reduced the cardiac rate pressure product (43%) from I/R heart. Importantly, diabetic I/R hearts also experienced a decline in the mitochondrial copy number (60%); downregulation in the expression of mitochondrial bioenergetics (ND1, ND2, ND3, ND4, ND5, ND6) and mitofusion (MFN1, MFN2) genes and the upregulation of mitophagy (PINK, PARKIN, OPTN) and mitofission (MFF, DNM1, FIS1) genes that reduce the dp/dt contribute to the contractile dysfunction in DM I/R hearts. Besides, a negative correlation was obtained between mitochondrial PGC1α, POLGA, TFAM genes and DNA hypermethylation in DM I/R hearts. Based on the above data, the elevated global DNA methylation level in diabetic I/R rat hearts deteriorated the mitochondrial function by downregulating the expression of POLGA, TFAM and PGC1α genes and negatively contributed to I/R-associated increased infarct size and altered hemodynamics. Full article

Climate change and rapid urbanization are two global processes that have significantly aggravated natural disasters, such as drought and flooding. Urbanization without resilient and sustainable planning and execution could lead to undesirable changes in landscapes and stormwater regulation capacity. These changes have exacerbated the effects of extreme climatic events with disastrous consequences in many cities worldwide. Unfortunately, the major storm in Zhengzhou, China on 20 July 2021 was one of these examples. This event provided a rare opportunity to study the key roles of green infrastructures (GI) in mitigating flooding risks in a major urban center after a devasting flood event. Using the data from high-resolution images collected via two satellites, a comprehensive study of the Jialu System in Greater Zhengzhou was conducted to systematically compare how far the river water had reached before and after the 20 July 2021 major storm in order to identify the main weak links in the city’s GI and stormwater management system. A flood inundation intensity index (FI) in the Upper (UJLR), Middle (MJLR), and Lower (LJLR) Regions of the Jialu River System was generated. Bivariate Moran’s I, a correlation coefficient between FI and landscape characteristics, was calculated and used to identify problem areas for future improvements. Our results showed that the MJLR had the severest flooding impacts. LJLR had the biggest change in how far the river water reached after flooding, ranging from 4.59 m to 706.28 m. In UJLR, the percentages of mine, crop land, and green space had the highest global bivariate Moran’s I correlation coefficients. In MJLR, the percentages of vacant land, impervious surfaces, and water body had the highest global bivariate Moran’s I correlation coefficients. In LJLR, the percentages of vacant land, water body, and crop land had the highest global bivariate Moran’s I correlation coefficients. The total percentages of both high landscape characteristics indices-high flood inundation intensity indices and low landscape characteristics indices-high flood inundation intensity indices areas are 12.96%, 13.47%, and 13.80% in UJLR, MJLR, and LJLR, respectively. These land cover composition types identified for each region can be treated as areas of primary focus. However, GeoDector Model (GDM) analyses showed that our eight variables of landscape characteristics were not independent. Hence, a more comprehensive approach integrating all eight variables is still necessary in future flood mitigation efforts. Full article

Bronchopulmonary dysplasia (BPD) is a morbid lung disease distinguished by lung alveolar and vascular simplification. Hyperoxia, an important BPD causative factor, increases extracellular signal-regulated kinases (ERK)-1/2 expression, whereas decreased lung endothelial cell ERK2 expression reduces angiogenesis and potentiates hyperoxia-mediated BPD in mice. However, ERK1′s role in experimental BPD is unclear. Thus, we hypothesized that hyperoxia-induced experimental BPD would be more severe in global ERK1-knockout (ERK1^-/-) mice than their wild-type (ERK1^+/+ mice) littermates. We determined the extent of lung development, ERK1/2 expression, inflammation, and oxidative stress in ERK1^-/- and ERK1^+/+ mice exposed to normoxia (FiO₂ 21%) or hyperoxia (FiO₂ 70%). We also quantified the extent of angiogenesis and hydrogen peroxide (H₂O₂) production in hyperoxia-exposed neonatal human pulmonary microvascular endothelial cells (HPMECs) with normal and decreased ERK1 signaling. Compared with ERK1^+/+ mice, ERK1^-/- mice displayed increased pulmonary ERK2 activation upon hyperoxia exposure. However, the extent of hyperoxia-induced inflammation, oxidative stress, and interrupted lung development was similar in ERK1^-/- and ERK1^+/+ mice. ERK1 knockdown in HPMECs increased ERK2 activation at baseline, but did not affect in vitro angiogenesis and hyperoxia-induced H₂O₂ production. Thus, we conclude ERK1 is dispensable for hyperoxia-induced experimental BPD due to compensatory ERK2 activation. Full article

The agricultural sector is facing a decrease in water supply and water quality at a global level and this is a problem that strictly affects all the Mediterranean olive growing areas. The aim of this work was to evaluate, for the first time, by NMR Spectroscopy and multivariate data analysis the metabolic profiling of the oils produced under different irrigation schemes. Arbosana olive oils were obtained from the use of saline reclaimed water (RW) and treated municipal wastewater (DW), combined with: full irrigation (FI) and regulated deficit irrigation (RDI). The results show a higher relative content of saturated fatty acids in EVOOs obtained from RDI strategy, regardless of the water source. Moreover, an increase in unsaturated fatty acids, a ω6/ω3 ratio content was observed in EVOOs obtained from RW when compared with DW water. Furthermore, the RW–RDI showed an increase in secoiridoid derivatives and hydroperoxides with respect to DW–RDI. A sustainable irrigation management, by combining a deficit irrigation strategy and saline reclaimed water source, could be crucial in order to overcome the problem of water scarcity and to guarantee the olive oil nutraceutical properties. The ¹H NMR-based metabolomic approach proved a powerful and versatile tool for this specific investigation. Full article

Reduced cognitive flexibility, characterized by restricted interests and repetitive behavior, is associated with atypical memory performance in autism spectrum disorder (ASD), suggesting hippocampal dysfunction. FOXP1 syndrome is a neurodevelopmental disorder characterized by ASD, language deficits, global developmental delay, and mild to moderate intellectual disability. Strongly reduced Foxp1 expression has been detected in the hippocampus of Foxp1^+/− mice, a brain region required for learning and memory. To investigate learning and memory performance in these animals, fear conditioning tests were carried out, which showed impaired associative learning compared with wild type (WT) animals. To shed light on the underlying mechanism, we analyzed various components of the mitochondrial network in the hippocampus. Several proteins regulating mitochondrial biogenesis (e.g., Foxo1, Pgc-1α, Tfam) and dynamics (Mfn1, Opa1, Drp1 and Fis1) were significantly dysregulated, which may explain the increased mitophagy observed in the Foxp1^+/− hippocampus. The reduced activity of complex I and decreased expression of Sod2 most likely increase the production of reactive oxygen species and the expression of the pre-apoptotic proteins Bcl-2 and Bax in this tissue. In conclusion, we provide evidence that a disrupted mitochondrial network and the resulting oxidative stress in the hippocampus contribute to the altered learning and cognitive impairment in Foxp1^+/− mice, suggesting that similar alterations also play a major role in patients with FOXP1 syndrome. Full article

Data are required for optimizing workplace design, assessing user experience, and ensuring wellbeing. This research focuses on the benefits of incorporating post-occupancy evaluation (POE) data analysis by studying the digital trail of employees generated by the existing Wi-Fi infrastructure of the office. The objective is to enable a safe return to offices through compliance with COVID-19 space-capacity regulations and in consideration of the health and wellbeing of employees. Workplaces, teams, and people have become more digitalized and therefore more mobile due to the globalization of knowledge and cutting-edge technological innovations, a process that has been accelerated by the COVID-19 crisis. Now, hybrid work and fully remote working routines are increasing in a significant number of companies. Nevertheless, with the return to the office, understanding how to calibrate spatial capacity is now key for workplaces and companies. Traditional assessment methods are obsolete; new methods that respond to mobility, changing occupancy rates, and comfort are essential. This paper analyzes, through the case study of a pre-COVID-19 activity-based office, the advantages of using digital indoor-location techniques (such as Wi-Fi networks, which additionally have the advantage of being previously installed in the majority of these spaces). The paper demonstrates that the incorporation of digital POE of user trends enabled a more seamless, accurate, and scalable return to a new normal office work scenario and an improved post-COVID-19 design of workplaces. Full article

Pyruvate is a central metabolite that connects many metabolic pathways in living organisms. To meet the cellular pyruvate requirements, the enterobacterium Escherichia coli has at least three pyruvate uptake systems—the H⁺/pyruvate symporter BtsT, and two thus far less well-characterized transporters, YhjX and CstA. BtsT and CstA belong to the putative carbon starvation (CstA) family (transporter classification TC# 2.A.114). We have created an E. coli mutant that cannot grow on pyruvate as the sole carbon source and used it to characterize CstA as a pyruvate transporter. Transport studies in intact cells confirmed that CstA is a highly specific pyruvate transporter with moderate affinity and is energized by a proton gradient. When cells of a reporter strain were cultured in complex medium, cstA expression was maximal only in stationary phase. A DNA affinity-capture assay combined with mass spectrometry and an in-vivo reporter assay identified Fis as a repressor of cstA expression, in addition to the known activator cAMP-CRP. The functional characterization and regulation of this second pyruvate uptake system provides valuable information for understanding the complexity of pyruvate sensing and uptake in E. coli. Full article

The energy demand of mankind is constantly growing, thus the utilization of various renewable energy sources, which also reduces negative environmental effects, is becoming more and more important. Because of the achievement of climate protection targets, photovoltaic (PV) energy has an increasing role in the global energy mix. This paper presents the technical and economic aspects of different photovoltaic system configurations designed to suit the Hungarian renewable energy regulations. In this study, five alternative PV configurations were examined for systems with a capacity from 50 kW to 500 kW, related to low- and medium-voltage installations. This article also introduces and explains the Hungarian economic PV and Feed-in-Tariff (FiT) regulations, where three different investment alternatives are analyzed with the help of economic indicators. This study could help stakeholders in the market (e.g., the Hungarian industry sector and local governments) understand the possible directions of technical and economic PV development. According to the results, the payback periods in all the studied economic-technical cases were below 10 years. The experimental results show that each investment option may be a good decision from an economic and technical point of view under the Hungarian regulations in force in 2019. Full article

Cumulus cells of pre-pubertal domestic animals are dysfunctional, perhaps due to age-specific epigenetic events. This study was designed to determine effects of melatonin treatment of donors on methylation modification of pre-pubertal cumulus cells. Cumulus cells from germinal vesicle stage cumulus oocyte complexes (COCs) were collected from eighteen lambs which were randomly divided into control group (C) and melatonin group given an 18 mg melatonin implant subcutaneous (M). Compared to the C group, the M group had higher concentrations of melatonin in plasma and follicular fluid (p < 0.05), greater superovulation, a higher proportion of fully expanded COCs, and a lower proportion of apoptotic cumulus cells (p < 0.05). Real-time PCR results showed that melatonin up-regulated expression of genes MT1, Bcl2, DNMT1, DNMT3a and DNMT3b, but down-regulated expression of genes p53, Caspase 3 and Bax (p < 0.05). Furthermore, melatonin increased FI of FITC (global methylation level) on cumulus cells (p < 0.05). To understand the regulation mechanism, the DNMTs promoter methylation sequence were analyzed. Compared to the C group, although there was less methylation at two CpG sites of DNMT1 (p < 0.05) and higher methylation at two CpG sites of DNMT3a (p < 0.05), there were no significant differences in methylation of the detected DNMT1 and DNMT3a promoter regions. However, there were lower methylation levels at five CpG sites of DNMT3b, which decreased methylation of detected DNMT3b promoter region on M group (p < 0.05). In conclusion, alterations of methylation regulated by melatonin may mediate development of cumulus cells in lambs. Full article