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Keywords = forest tree species classification

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31 pages, 29169 KB  
Article
Domain-Adapted Supervised Learning for Tree Species Mapping Using UAV Multispectral Data
by Sowmya Natesan, Udayalakshmi Vepakomma and Costas Armenakis
Forests 2026, 17(7), 738; https://doi.org/10.3390/f17070738 - 25 Jun 2026
Viewed by 240
Abstract
Individual tree species classification is essential for detailed forest inventories, ecosystem monitoring, and biodiversity assessment. While UAV-acquired RGB and multispectral (MS) imagery have advanced tree species mapping, most studies focus on a single sensor type. In practice, UAV platforms carry diverse sensors with [...] Read more.
Individual tree species classification is essential for detailed forest inventories, ecosystem monitoring, and biodiversity assessment. While UAV-acquired RGB and multispectral (MS) imagery have advanced tree species mapping, most studies focus on a single sensor type. In practice, UAV platforms carry diverse sensors with varying spatial resolutions, spectral bands, radiometric responses, and noise characteristics, introducing domain shifts that limit model generalization across datasets. To overcome these challenges, we propose a supervised cross-sensor transfer learning approach, leveraging a DenseNet-121 model pretrained on high-resolution UAV RGB imagery to improve classification on lower-resolution multispectral imagery with limited labelled data. The adapted model achieved 75% overall accuracy and a macro-F1 score of 0.706, significantly improving over models trained from scratch. Its performance was further evaluated on downsampled UAV MS imagery simulating conventional airborne multispectral photographs, demonstrating robustness and practical applicability for regional-scale forest inventories. This study highlights cross-domain transfer learning as a pathway toward sensor-independent, efficient, and operationally scalable tree species classification. Full article
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29 pages, 9857 KB  
Article
Network Structure Explained the Differences in the Response of Soil Bacterial Community Structure and Functional Structure to Afforestation Types
by Zhenlu Qiu, Jin Liu, Hui Gao, Suying Dong, Xiaojin Zang, Wenxin Kang and Jing Shu
Forests 2026, 17(6), 702; https://doi.org/10.3390/f17060702 - 16 Jun 2026
Viewed by 276
Abstract
This study used 16S rDNA high-throughput sequencing and Faprotax functional prediction to analyze the effects of different artificial forests (coniferous forest, conifer–broad-leaved mixed forest, broad-leaved forest) in the Fanggan ecological restoration area of North China on soil bacterial community composition and functional characteristics [...] Read more.
This study used 16S rDNA high-throughput sequencing and Faprotax functional prediction to analyze the effects of different artificial forests (coniferous forest, conifer–broad-leaved mixed forest, broad-leaved forest) in the Fanggan ecological restoration area of North China on soil bacterial community composition and functional characteristics and, based on network topology features, analyzed the potential influencing pathways. Planting broad-leaved forests significantly increased soil bacterial α-diversity indices (ACE, Chao1, Shannon) and induced the greatest heterogeneity in both community and functional composition. Soil bacteria exhibit significant differences in taxonomic structure across forest types but not in functional structure. The classification network and functional network of broad-leaved forests are more complex than those of coniferous and mixed forests, with the former having more nodes and edges, as well as higher weighted degree and betweenness centrality. Zi-Pi analysis indicates that high-abundance taxa involved in carbon and nitrogen cycles dominate the keystone taxa of the taxonomic network, while low-abundance pathogenic, urea-decomposing, and trace element metabolism functional groups dominate the keystone groups of the functional network. Redundancy analysis further revealed that soil available potassium concentration, pH, and tree species composition (importance values of Pinus tabulaeformis and Populus davidiana) were the principal determinants of bacterial functional structure. Collectively, broad-leaved forests achieve higher network robustness via elevated network complexity and functional redundancy, whereas coniferous forests might rely on functional convergence and modular integration to cope with resource limitation. These results indicate that network traits mediate the distinct responses of bacterial communities and their functional potentials, offering practical references for vegetation restoration in limestone mountain areas. Full article
(This article belongs to the Section Forest Soil)
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18 pages, 15664 KB  
Article
Subpixel Mapping of Flammable Tree Species in Yajiang County Based on Sentinel-2 Time-Series Data and a Spectral Mixing–Unmixing Strategy
by Zhiqiang Li, Xiaobing Deng, Dongzhou Deng, Yue Wang, Ling Wu, Wenyan Yu, Bingnan Dong and Ben Yang
Remote Sens. 2026, 18(12), 1952; https://doi.org/10.3390/rs18121952 - 12 Jun 2026
Viewed by 291
Abstract
The spatial distribution of flammable tree species directly influences forest fuel structure and fire risk patterns. However, mixed pixels limit the ability of conventional classification methods to characterize continuous within-pixel variation in species composition, thereby constraining fine-scale forest mapping. To address this issue, [...] Read more.
The spatial distribution of flammable tree species directly influences forest fuel structure and fire risk patterns. However, mixed pixels limit the ability of conventional classification methods to characterize continuous within-pixel variation in species composition, thereby constraining fine-scale forest mapping. To address this issue, this study developed a subpixel mapping framework for flammable tree species in Yajiang County, Sichuan Province, by integrating Sentinel-2 time-series data with a spectral mixing–unmixing strategy. Using 2019 Sentinel-2 time-series data and National Forest Inventory (NFI) data, temporal mixed samples with known abundance fractions were generated using a linear spectral mixing model. An XGBoost-based collaborative multi-regression framework was then applied to estimate the proportions of different tree-species endmembers within complex forest pixels. Quantitative evaluation using synthetic mixed samples showed that the model achieved stable unmixing performance across different random mixing scenarios. The best performance was obtained under the Mixed 2 scenario with a sample size of 250 K, reaching an R2 of 0.821. The resulting maps revealed continuous spatial variation in the abundance and composition of flammable tree species. Mountain pine was the most widespread and dominant species, followed by spruce and mountain oak, whereas birch and fir mainly exhibited localized patchy distributions. An additional NFI-based categorical evaluation assessed the consistency of the final maps with real forest inventory records. The identification accuracies were 93.95% for pure stands and 91.22% for mixed stands, while the species classification accuracies were 87.28% for pure stands and 84.41% for dominant species in mixed stands. The proposed framework provides useful spatial information for regional forest fuel assessment and fire risk management. Full article
(This article belongs to the Section Forest Remote Sensing)
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24 pages, 67340 KB  
Article
Evaluating the Influence of Pseudo Tree Crown (PTC) Input Alternatives for Machine Learning and Deep Learning Models on Individual Tree Classification Performance
by Tong Yan, Kongwen Zhang, Wuxue Cheng and Jane Liu
Remote Sens. 2026, 18(11), 1848; https://doi.org/10.3390/rs18111848 - 4 Jun 2026
Viewed by 239
Abstract
Individual tree classification has a long history of diverse development, with recent trends focusing on the adoption of machine learning and deep learning approaches. It is a simple and powerful approach that allows the model to auto-pilot while reducing the need for physical [...] Read more.
Individual tree classification has a long history of diverse development, with recent trends focusing on the adoption of machine learning and deep learning approaches. It is a simple and powerful approach that allows the model to auto-pilot while reducing the need for physical characteristic understanding. Over more than a decade of research, we have focused on establishing a direct representation of individual trees that bridges 2D top-down imagery and true 3D models. In this study, we investigated the fundamental question of the influence of the input data on these ML/DL models. In 2024, we introduced a novel data transformation method, the Pseudo Tree Crown (PTC), which provides a pseudo-3D pixel-value perspective that enhances the informational richness of images and significantly improves classification performance. Our original implementation was successfully tested on urban and deciduous trees in 2024 and was later extended to Canadian natural conifer species under snow conditions in 2025. However, the original PTC relied on the green band, limiting its applicability to green-leaf species. In this study, we analyzed and compared the performance of different data variations and transformations, such as the Green–Red Vegetation Index (GRVI) and principal component analysis (PCA), as direct input and used their PTC forms. Classifications were conducted using Random Forest (RF), ResNet50, YOLOv10 and Segment Anything (SA). The results confirmed the effectiveness of the PTC, which consistently improves the classification accuracy by at least 5% without introducing additional computational time or complexity. Furthermore, PTC exhibits robust, consistent behavior across all data forms, demonstrating its strong resilience and reliability. Full article
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18 pages, 43774 KB  
Article
Automatic Tree Species Identification in a Cold Temperate Natural Broadleaf Mixed Forest Using High-Resolution UAV Imagery and Mask R-CNN
by Vladislav Bukin, Maximo Larry Lopez Caceres, Yago Diez Donoso, Takashi Kobayashi, Le Tien Nguyen, Friederich Blum, Muhammad Iqbal Faishal and Anna Trigubenko
Remote Sens. 2026, 18(11), 1692; https://doi.org/10.3390/rs18111692 - 23 May 2026
Viewed by 342
Abstract
Forest ecosystems in northeastern Japan are characterized by natural mixed forests, where sustainable management has always been limited because of their difficult accessibility. The aims of this study are first, to assess mixed forest composition, and second, to train Mask R-CNN models with [...] Read more.
Forest ecosystems in northeastern Japan are characterized by natural mixed forests, where sustainable management has always been limited because of their difficult accessibility. The aims of this study are first, to assess mixed forest composition, and second, to train Mask R-CNN models with these data in order to detect and segment trees in a 19-ha mixed forest composed mainly of beech (Fagus crenata), oak (Quercus crispula), magnolia (Magnolia obovata) and larch (Larix kaempferi). The Mask R-CNN model was applied in two experimental scenarios: a single multi-class model and species-specific models. RGB images consisted of four orthomosaics (August, September, October 2024 and October 2025), which yielded 1725, 359, 129 and 525 samples of each tree species, respectively. The Unmanned Aerial Vehicle (UAV)-QField validation method improved the classification accuracy of the annotations and made it possible to map each tree species distribution and understand the composition of mixed forests along an elevation gradient. The multi-class model demonstrated an overall precision of 0.59, a recall of 0.53, and an F1-score of 0.56. The detection performance for individual tree species was similar for both models. Based on these results, the multi-class model is more suitable because it decreases the possibility of misclassification of tree species. Full article
(This article belongs to the Section Forest Remote Sensing)
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20 pages, 4455 KB  
Article
Soil Organic Carbon Storage in Temperate Forests: Utilizing of the Forestry Site Classification and the Role of Main Tree Species
by Vít Šrámek, Kateřina Neudertová Hellebrandová, Ondřej Špulák and Věra Fadrhonsová
Forests 2026, 17(5), 547; https://doi.org/10.3390/f17050547 - 29 Apr 2026
Viewed by 330
Abstract
Soil organic carbon (SOC) storage in forests is governed by complex interactions between site conditions and vegetation. This study quantifies SOC stocks across a gradient of Target Management Sets (TMS) in the Czech Republic (Central Europe) to evaluate the baseline storage capacity of [...] Read more.
Soil organic carbon (SOC) storage in forests is governed by complex interactions between site conditions and vegetation. This study quantifies SOC stocks across a gradient of Target Management Sets (TMS) in the Czech Republic (Central Europe) to evaluate the baseline storage capacity of distinct ecological sites and the modifying effects of dominant tree species, specifically Norway spruce and European beech. Utilizing large-scale spatial data, linear mixed-effects models, and piecewise structural equation modeling (pSEM), we analyzed SOC stratification across middle (≈400–600 m a.s.l.) and higher (≈600–800 m a.s.l.) elevational zones. The results indicate that while overall SOC stocks inherently increase with elevation due to climatic constraints, tree species dictate the vertical carbon distribution within the soil profile. Specifically, conifers (i.e., Norway spruce and Scots pine) accumulate SOC primarily in the organic layer, whereas broadleaves (mainly European beech and oak) translocate and stabilize carbon in deeper mineral horizons. The pSEM analysis revealed that beech functions as a ‘calcium pump’, increasing topsoil pH and driving calcium-mediated SOC stabilization in mineral soils. This mechanism is highly effective at middle elevations but partially overridden by abiotic limits at higher elevations. We conclude that inherent site conditions (TMS) determine total SOC capacity, whereas tree species management controls SOC stability. Although no significant differences were observed in total SOC stocks between conifers and broadleaves at the same sites (medians of total SOC ranged from approx. 5 to 16 kg·m−2, depending on the site), converting purely coniferous stands into broadleaves represents an effective strategy for long-term mineral SOC stabilization, particularly in middle-elevation sites. Full article
(This article belongs to the Section Forest Ecology and Management)
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32 pages, 7017 KB  
Article
Individual Tree Species Classification in a Mining Area of the Yellow River Basin Using UAV-Based LiDAR, Hyperspectral, and RGB Data
by Guo Wang, Sheng Nie, Xiaohuan Xi, Cheng Wang and Hongtao Wang
Remote Sens. 2026, 18(9), 1361; https://doi.org/10.3390/rs18091361 - 28 Apr 2026
Viewed by 525
Abstract
The Yellow River Basin contains abundant coal resources; however, its ecological environment is inherently fragile, and vegetation degradation has been further intensified by extensive mining activities. Accurate classification of individual tree species in mining-affected areas is therefore essential for assessing ecological conditions and [...] Read more.
The Yellow River Basin contains abundant coal resources; however, its ecological environment is inherently fragile, and vegetation degradation has been further intensified by extensive mining activities. Accurate classification of individual tree species in mining-affected areas is therefore essential for assessing ecological conditions and establishing a scientific foundation for targeted restoration and sustainable management. To address this need, an evaluated machine learning framework was developed and evaluated for individual tree species classification in a coal mining area of the Yellow River Basin using integrated unmanned aerial vehicle (UAV) data. A comprehensive feature set was constructed by extracting 278 attributes per tree. These attributes included 224 spectral bands and 29 hyperspectral indices derived from hyperspectral imagery, 24 textural metrics obtained from RGB orthophotos, and one canopy height feature generated from a LiDAR-derived model. Based on ground-truth data from 1095 individual trees, seven machine learning algorithms were trained and systematically compared: Random Forest (RF), Support Vector Machine (SVM), K-Nearest Neighbors (KNN), Decision Tree (DT), Gradient Boosting (GB), Logistic Regression (LR), and XGBoost. Statistical significance testing using 5 × 5 repeated cross-validation, together with the Friedman test and post hoc Nemenyi test, and additional model stability analysis consistently identified XGBoost as the optimal classifier. On an independent test set, XGBoost achieved high accuracy (Overall Accuracy = 0.897, Kappa = 0.811) with an efficient training time of 2.36 s. Further analysis demonstrated the critical and complementary roles of hyperspectral and structural features in species discrimination. The optimized model was subsequently applied to generate a detailed wall-to-wall tree species map across the entire mining area. Overall, this study presents a statistically informed comparison of classifiers for multi-source feature-based species discrimination and delivers an evaluated and practical pipeline for effective vegetation monitoring. The proposed framework provides a scientific tool for assessing and managing ecological recovery in complex mining environments, particularly within ecologically sensitive regions such as the Yellow River Basin. Full article
(This article belongs to the Special Issue Remote Sensing and Smart Forestry (Third Edition))
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25 pages, 5188 KB  
Article
MonoCrown for Crown-Level Tree Species Semantic Segmentation in Heterogeneous Forests Using UAV RGB Imagery
by Linzhi Wen and Guangsheng Chen
Remote Sens. 2026, 18(9), 1338; https://doi.org/10.3390/rs18091338 - 27 Apr 2026
Cited by 1 | Viewed by 474
Abstract
Crown-level tree species semantic segmentation enables fine-grained forest inventory and management. Current high-precision tree species classification typically relies on multi-source remote sensing data, the acquisition and processing of which remain costly for large-area applications, making low-cost unmanned aerial vehicle (UAV) RGB imagery an [...] Read more.
Crown-level tree species semantic segmentation enables fine-grained forest inventory and management. Current high-precision tree species classification typically relies on multi-source remote sensing data, the acquisition and processing of which remain costly for large-area applications, making low-cost unmanned aerial vehicle (UAV) RGB imagery an attractive option for large-scale forest mapping. However, in heterogeneous forests, complex canopy structures and the limited spectral discriminability of low-cost UAV RGB imagery make 2D appearance cues alone insufficient for reliable species discrimination, crown delineation, and accurate separation of adjacent crowns. This often leads to inter-class confusion, blurred crown boundaries, and poor recognition of small crowns. To address these limitations, this paper proposes MonoCrown (MCrown), which strengthens geometric and contextual representation for distinguishing visually similar species and delineating crowns from single-temporal UAV RGB imagery. To compensate for the insufficiency of appearance cues, MCrown introduces monocular depth inferred offline from the same RGB image as a frozen geometric prior, and integrates cross-window global–local attention (CW-GLA), bidirectional cross-modal attention (BiCoAttn), and depth-adaptive injection (DAI) to capture long-range dependencies and promote complementary use of appearance and geometric features, especially for small crowns with similar visual patterns in complex scenes. To validate the method’s effectiveness, a crown-level UAV RGB dataset covering approximately 40 km2 was constructed. Systematic comparative experiments were conducted on the proposed dataset and on public benchmarks, supporting the effectiveness of the proposed approach across ten dominant classes, especially for small crowns and visually similar categories. Its mean Intersection over Union (mIoU) and overall accuracy (OA) reached 74.1% and 87.3%, respectively. The method achieves high-precision crown-level tree species semantic segmentation using single-temporal UAV RGB as the sole acquired modality, while monocular depth inferred from the same RGB image serves only as a frozen geometric prior, without requiring multispectral, multi-temporal, or active-sensor acquisitions. This offers a practical solution for crown-level tree species mapping in heterogeneous forests. Full article
(This article belongs to the Section Remote Sensing Image Processing)
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30 pages, 3811 KB  
Article
FA-CTNet: A Geometry-Aware Deep Learning Approach for Tree Species Classification from LiDAR Point Clouds
by Shengchao Sha, Qianhui Liu, Yan Zhang and Ting Yun
Remote Sens. 2026, 18(9), 1311; https://doi.org/10.3390/rs18091311 - 24 Apr 2026
Viewed by 452
Abstract
Accurate identification of tree species is important for forest management, biodiversity studies, and precision forestry. Near-range LiDAR point clouds provide detailed three-dimensional information about individual trees. However, the complex structure of the point clouds and the unbalanced distribution of species make automatic classification [...] Read more.
Accurate identification of tree species is important for forest management, biodiversity studies, and precision forestry. Near-range LiDAR point clouds provide detailed three-dimensional information about individual trees. However, the complex structure of the point clouds and the unbalanced distribution of species make automatic classification difficult. To address these issues, this study presents a Transformer model with geometric enhancement. The model combines local geometric features and global attention to improve species recognition in forest environments. It uses geometric information with biological meaning, including point cloud normals, local density, vertical structure, and growth direction. A focal loss with class balance is also introduced to reduce the impact of species distributions with long tails. Experiments on the ForSpecial20K dataset show that the proposed method performs better than representative models based on convolution, graph methods, and Transformer architectures. It achieves higher overall accuracy (78.20%), higher mean class accuracy (73.48%), and a higher Macro-F1 score (73.21%). Results from confusion matrices and visual analysis of similar species further verify the effectiveness of the geometric features and the loss design. These results suggest that modeling structural information of forests helps improve robustness and generalization. The proposed method offers a practical solution for tree-level species mapping, fusion of LiDAR data from multiple sources, and fine-scale forest inventory. It also shows the value of combining high-resolution LiDAR data with deep learning for forestry applications. Full article
(This article belongs to the Section Forest Remote Sensing)
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15 pages, 4945 KB  
Article
Evaluation of Deep Learning Models for Image-Based Classification of Timber Logs by Market Value
by Matevž Triplat, Žiga Lukančič and Vasja Kavčič
Forests 2026, 17(5), 518; https://doi.org/10.3390/f17050518 - 23 Apr 2026
Viewed by 447
Abstract
The identification of standing tree species, timber logs, and on-site assessment of their quality and value using images holds significant potential for forestry applications, including inventory management, traceability under EU regulations like the Deforestation Regulation, and market valuation amid growing demands for sustainable [...] Read more.
The identification of standing tree species, timber logs, and on-site assessment of their quality and value using images holds significant potential for forestry applications, including inventory management, traceability under EU regulations like the Deforestation Regulation, and market valuation amid growing demands for sustainable practices. This study addresses this by classifying images of timber logs by tree species and market value using the Orange data mining software, which leverages pre-trained convolutional neural networks (Inception v3 and SqueezeNet) to generate embeddings from a dataset of 5549 images collected at a real timber auction in Slovenia, followed by logistic regression image classification. Results show high accuracy for tree species classification (up to 92.6%), but substantially lower accuracy for market value classification (40%–55%), reflecting the greater complexity of value determination from visual features. These findings underscore the promise of deep learning for species identification while indicating the need for further methodological advancements to enhance value classification reliability, which offers the practical impact for operational forestry and bioeconomy value chains. Full article
(This article belongs to the Special Issue Sustainable Forest Operations: Technology, Management, and Challenges)
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32 pages, 3275 KB  
Article
Machine Learning-Based Mapping of Dominant Tree Species in Dryland Forests Using Multi-Temporal and Multi-Source Data
by Emad H. E. Yasin, Milan Koreň and Kornel Czimber
Remote Sens. 2026, 18(8), 1185; https://doi.org/10.3390/rs18081185 - 15 Apr 2026
Viewed by 463
Abstract
Timely and accurate mapping of tree species is essential for forest resource inventory, biodiversity conservation, and sustainable ecosystem management, particularly in dryland environments where structural heterogeneity, spectral similarity, and data scarcity complicate classification. This study develops a machine learning-based framework implemented in Google [...] Read more.
Timely and accurate mapping of tree species is essential for forest resource inventory, biodiversity conservation, and sustainable ecosystem management, particularly in dryland environments where structural heterogeneity, spectral similarity, and data scarcity complicate classification. This study develops a machine learning-based framework implemented in Google Earth Engine to map dominant tree species in the Elnour Natural Forest Reserve (ENFR), Blue Nile, Sudan, using multi-temporal and multi-sensor remote sensing data. Multi-temporal Landsat 5 TM, Landsat 8 OLI, and Sentinel-2 MSI imagery were integrated with vegetation index (NDVI), topographic variables derived from a digital elevation model (DEM), and field observations. The performance of Random Forest (RF), Support Vector Machine (SVM), Classification and Regression Trees (CART), and an unweighted ensemble approach was evaluated across four reference years (2008, 2013, 2018, and 2021). Results show that RF and SVM consistently achieved high classification performance, with overall accuracy (OA) ranging from 85.0% to 92.0% and Kappa coefficients (κ) from 0.81 to 0.89, while maintaining stable and ecologically realistic species-area estimates. CART showed greater sensitivity to class imbalance and overestimated minor species (OA = 72.0–80.0%, κ = 0.65–0.74), whereas the ensemble approach amplified misclassification of rare classes (OA = 78.0–84.0%, κ = 0.70–0.78). The integration of Sentinel-2 data improved species discrimination due to enhanced spatial and spectral resolution, particularly in the red-edge region; however, algorithm selection remained the dominant factor controlling performance. Feature importance analysis identified near-infrared (NIR), shortwave infrared (SWIR), and NDVI variables as the most influential predictors. Multi-temporal analysis revealed declining class separability, reflected by decreasing MCC values, and a shift in species composition, including a decline in Acacia seyal (Delile) and an increase in Sterculia setigera Delile. These patterns indicate increasing ecological complexity driven primarily by anthropogenic pressures, with climatic variability acting as an additional stressor. Full article
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25 pages, 45583 KB  
Article
Terrain-Aware Self-Supervised Representation Learning for Tree Species Mapping in Mountainous Regions Under Limited Field Samples
by Li He, Leiguang Wang, Liang Hong, Qinling Dai, Wei Gu, Xingyue Du, Mingqi Yang, Juanjuan Liu and Yaoming Feng
Remote Sens. 2026, 18(6), 951; https://doi.org/10.3390/rs18060951 - 21 Mar 2026
Viewed by 497
Abstract
Accurate tree species mapping is critical for forest inventory, biodiversity assessment, and ecosystem management. In mountainous regions, terrain-induced radiometric non-stationarity and limited field access often produce scarce, clustered, and environmentally biased samples, limiting model generalization. To address this issue, this study proposes a [...] Read more.
Accurate tree species mapping is critical for forest inventory, biodiversity assessment, and ecosystem management. In mountainous regions, terrain-induced radiometric non-stationarity and limited field access often produce scarce, clustered, and environmentally biased samples, limiting model generalization. To address this issue, this study proposes a terrain-aware self-supervised representation learning framework for tree species classification under small-sample conditions. The framework integrates terrain information into representation learning and adopts a hybrid contrastive–generative self-supervised strategy to learn discriminative and terrain-robust features from large volumes of unlabeled multi-source remote sensing data. These learned representations are subsequently combined with limited field samples to produce regional-scale tree species maps. Experiments conducted across Yunnan Province, China, using Sentinel-1, Sentinel-2 and Landsat time-series data show that the proposed framework substantially improvesa class separability and classification robustness in complex mountainous environments. The framework achieves an overall accuracy of 75.8%, significantly outperforming conventional feature engineering (38.3–40.6%) and supervised deep learning models (37.3–47.8%). Species with relatively homogeneous structure and strong ecological niche dependence can be accurately mapped with limited training samples, whereas structurally complex forest communities require broader environmental sample coverage. Overall, the results highlight the potential of terrain-aware self-supervised representation learning as a scalable and data-efficient paradigm for forest mapping in mountainous and environmentally heterogeneous regions. Full article
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25 pages, 10673 KB  
Article
Application of UAV Devices to Assess Post-Drought Canopy Vigor in Two Pine Forests Showing Die-Off
by Elisa Tamudo, Jesús Revuelto, Antonio Gazol and Jesús Julio Camarero
Remote Sens. 2026, 18(6), 916; https://doi.org/10.3390/rs18060916 - 17 Mar 2026
Viewed by 549
Abstract
Rising temperatures and droughts are triggering forest die-off in climate warming hotspots such as the Mediterranean Basin. UAVs equipped with LiDAR and multispectral sensors offer a powerful tool for surveys of tree vigor at landscape level. We used UAV-acquired LiDAR data and multispectral [...] Read more.
Rising temperatures and droughts are triggering forest die-off in climate warming hotspots such as the Mediterranean Basin. UAVs equipped with LiDAR and multispectral sensors offer a powerful tool for surveys of tree vigor at landscape level. We used UAV-acquired LiDAR data and multispectral camera imagery to segment individual tree crowns, classify species, and assess the health status in two drought-affected forests in northeastern Spain: a mixed Pinus pinasterQuercus ilex forest and a Pinus halepensis forest. Individual trees were segmented and classified using object-based image analysis with the Random Forest algorithm incorporating spectral, structural, and topographic variables. Greenness indices (NDVI and EVI) were analyzed in relation to crown height, topography (slope and elevation) and solar radiation, and their interactions. Analyses showed satisfactory crown segmentation (F-Score = 0.85–0.86) and species classification (Overall accuracy = 0.86–0.99), though distinguishing spectrally similar classes remained challenging. Taller P. pinaster trees exhibited higher NDVI, while taller P. halepensis displayed higher NDVI values in dense neighborhoods and on gentle slopes. These findings highlight the potential of high-resolution UAV-based remote sensing for effective near-real-time detection and attribution of forest die-off. Future research should aim to improve algorithm accuracy and better integrate field-based validation across different forest types. Full article
(This article belongs to the Special Issue Vegetation Mapping through Multiscale Remote Sensing)
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18 pages, 2917 KB  
Article
Phylogenetic Relationships of Five Phallales Species Based on Mitochondrial Genome Analysis
by Yaping Wang, Dan Li, Guoyu Wang, Zhongyao Guo, Xianyi Wang and Hongmei Liu
J. Fungi 2026, 12(3), 207; https://doi.org/10.3390/jof12030207 - 13 Mar 2026
Viewed by 1009
Abstract
Fungi of the Phallales order are globally distributed and are important in forest ecosystems, and many species have medicinal and edible value. However, despite the rich diversity, the information on this order is limited, and its taxonomic classification remains contentious. In this study, [...] Read more.
Fungi of the Phallales order are globally distributed and are important in forest ecosystems, and many species have medicinal and edible value. However, despite the rich diversity, the information on this order is limited, and its taxonomic classification remains contentious. In this study, the mitogenomes of five species from the Phallales order were sequenced, assembled, annotated, and compared. All five assembled mitogenomes were circular, ranging in size from 41,465 bp to 99,150 bp. Introns and intergenic regions were the key factors for mitogenome size variation in the Phallales order. The arrangement of 15 protein-coding genes, 2 rRNA genes, and 24 tRNA genes was highly conserved among the Phallales species. The only variation observed was the presence of an additional copy of trnI, trnT, trnD, and trnF in some mitogenomes. Specifically, the mitogenomes of P. rugulosus, P. hadriani, P. rigidiindusiatus, and P. dongsun had an additional copy of trnI, trnT, trnD, and trnF, respectively. A phylogenetic analysis produced well-supported phylogenetic tree, indicating that the mitogenome was an effective molecular marker for inferring evolutionary relationships. The phylogenetic analysis showed that the Phallales and Gomphales species share a closer evolutionary relationship. Our results contribute to a better understanding of the evolutionary dynamics, genetic constitution, and systematic classification of this important fungal community. Full article
(This article belongs to the Section Fungal Evolution, Biodiversity and Systematics)
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32 pages, 9091 KB  
Article
Multi-Temporal Fusion of Sentinel-1 and Sentinel-2 Data for High-Accuracy Tree Species Identification in Subtropical Regions
by Hui Li, Caijuan Luo, Xuan Kang, Haijun Luan and Lanhui Li
Remote Sens. 2026, 18(4), 592; https://doi.org/10.3390/rs18040592 - 13 Feb 2026
Cited by 1 | Viewed by 798
Abstract
Persistent cloud cover and frequent rainfall in subtropical regions throughout the year significantly limit the applicability of optical remote sensing for tree species identification, thereby constraining dynamic forest monitoring and precise management of forest resources. To address this challenge, this study proposes a [...] Read more.
Persistent cloud cover and frequent rainfall in subtropical regions throughout the year significantly limit the applicability of optical remote sensing for tree species identification, thereby constraining dynamic forest monitoring and precise management of forest resources. To address this challenge, this study proposes a tree species identification method that integrates multi-source remote sensing temporal features. By combining multi-temporal optical imagery from Sentinel-2 and dual-polarisation Synthetic Aperture Radar (SAR) data from Sentinel-1, we constructed a comprehensive feature set that incorporates spectral, structural, and phenological attributes, including various vegetation indices, backscatter coefficients, and polarimetric decomposition parameters. Through correlation analysis and assessment of temporal feature variability, five distinct integration strategies (T1-T5) were developed to classify six typical subtropical tree species: Pinus massoniana, Pinus elliottii, Acacia, Eucalyptus grandis, Mangrove, and Other hardwoods, using a random forest classifier. The results indicate that the multi-source feature fusion approach significantly outperforms single-source models, with the T5 strategy achieving the highest overall accuracy (OA) of 95.33% and a Kappa coefficient of 0.94. The red-edge vegetation indices and SAR polarimetric features were identified as major contributors to improving the classification accuracy of hardwood species. This study demonstrates that multi-source remote sensing data fusion can effectively mitigate the spatiotemporal constraints of optical imagery, providing a viable solution and technical framework for high-accuracy remote sensing classification in complex subtropical forest environments. Full article
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