Search Results (2,059)

Root phenology significantly influences ecosystem processes yet remains poorly characterized across biomes. This study synthesized data from 59 studies spanning Arctic to tropical ecosystems to identify woody plants root phenological patterns and their environmental drivers. The analysis revealed distinct climate-specific patterns. Arctic regions had a short growing season with remarkably low temperature threshold for initiation of root growth (0.5–1 °C). Temperate forests displayed pronounced spring-summer growth patterns with root growth initiation occurring at 1–9 °C. Mediterranean ecosystems showed bimodal patterns optimized around moisture availability, and tropical regions demonstrate seasonality primarily driven by precipitation. Root-shoot coordination varies predictably across biomes, with humid continental ecosystems showing the highest synchronous above- and belowground activity (57%), temperate regions exhibiting leaf-before-root emergence (55%), and Mediterranean regions consistently showing root-before-leaf patterns (100%). Winter root growth is more widespread than previously recognized (35% of studies), primarily in tropical and Mediterranean regions. Temperature thresholds for phenological transitions vary with climate region, suggesting adaptations to environmental conditions. These findings provide a critical, region-specific framework for improving models of terrestrial ecosystem responses to climate change. While our synthesis clarifies distinct phenological strategies, its conclusions are drawn from data focused primarily on Northern Hemisphere woody plants, highlighting significant geographic gaps in our current understanding. Bridging these knowledge gaps is essential for accurately forecasting how belowground dynamics will influence global carbon sequestration, nutrient cycling, and ecosystem resilience under changing climatic regimes. Full article

Timely and accurate monitoring of crop nitrogen (N) status is essential for precision agriculture. UAV-based hyperspectral remote sensing offers high-resolution data for estimating plant nitrogen concentration (PNC), but its cost and complexity limit large-scale application. This study compares the performance of UAV hyperspectral data (S185 sensor) with simulated multispectral data from DJI Phantom 4 Multispectral (P4M), PlanetScope (PS), and Sentinel-2A (S2) in estimating winter wheat PNC. Spectral data were collected across six growth stages over two seasons and resampled to match the spectral characteristics of the three multispectral sensors. Three variable selection strategies (one-dimensional (1D) spectral reflectance, optimized two-dimensional (2D), and three-dimensional (3D) spectral indices) were combined with Random Forest Regression (RFR), Support Vector Machine Regression (SVMR), and Partial Least Squares Regression (PLSR) to build PNC prediction models. Results showed that, while hyperspectral data yielded slightly higher accuracy, optimized multispectral indices, particularly from PS and S2, achieved comparable performance. Among models, SVM and RFR showed consistent effectiveness across strategies. These findings highlight the potential of low-cost multispectral platforms for practical crop N monitoring. Future work should validate these models using real satellite imagery and explore multi-source data fusion with advanced learning algorithms. Full article

To address the problems of traditional methods that rely on destructive sampling, the poor adaptability of fixed equipment, and the susceptibility of single-view angle measurements to occlusions, a non-destructive and portable device for three-dimensional phenotyping and biomass detection in lettuce was developed. Based on the Structure-from-Motion Multi-View Stereo (SFM-MVS) algorithms, a high-precision three-dimensional point cloud model was reconstructed from multi-view RGB image sequences, and 12 phenotypic parameters, such as plant height, crown width, were accurately extracted. Through regression analyses of plant height, crown width, and crown height, and the R² values were 0.98, 0.99, and 0.99, respectively, the RMSE values were 2.26 mm, 1.74 mm, and 1.69 mm, respectively. On this basis, four biomass prediction models were developed using Adaptive Boosting (AdaBoost), Support Vector Regression (SVR), Gradient Boosting Decision Tree (GBDT), and Random Forest Regression (RFR). The results indicated that the RFR model based on the projected convex hull area, point cloud convex hull surface area, and projected convex hull perimeter performed the best, with an R² of 0.90, an RMSE of 2.63 g, and an RMSEn of 9.53%, indicating that the RFR was able to accurately simulate lettuce biomass. This research achieves three-dimensional reconstruction and accurate biomass prediction of facility lettuce, and provides a portable and lightweight solution for facility crop growth detection. Full article

High moisture content (MC) harms wheat storage quality and readily leads to mold growth. Accurate localization of abnormal/high-moisture regions enables early warning, ensuring proper storage and reducing economic losses. The present study introduces the 2D microwave scanning method and investigates a novel localization method for addressing such a challenge. Both static and scanning experiments were performed on a developed mobile and non-destructive microwave detection system to quantify the MC of wheat and then locate abnormal moisture regions. For quantifying the wheat’s MC, a dual-parameter wheat MC prediction model with the random forest (RF) algorithm was constructed, achieving a high accuracy (R² = 0.9846, MSE = 0.2768, MAE = 0.3986). MC scanning experiments were conducted by synchronized moving waveguides; the maximum absolute error of MC prediction was 0.565%, with a maximum relative error of 3.166%. Furthermore, both one- and two-dimensional localizing methods were proposed for localizing abnormal moisture regions. The one-dimensional method evaluated two approaches—attenuation value and absolute attenuation gradient—using computer simulation technology (CST) modeling and scanning experiments. The experimental results confirmed the superior performance of the absolute gradient method, with a center detection error of less than 12 mm in the anomalous wheat moisture region and a minimum width detection error of 1.4 mm. The study performed two-dimensional antenna scanning and effectively imaged the high-MC regions using phase delay analysis. The imaging results coincide with the actual locations of moisture anomaly regions. This study demonstrated a promising solution for accurately localizing the wheat’s abnormal/high-moisture regions with the use of an emerging microwave transmission method. Full article

Archaeological sites located on the edge of growing cities often struggle to reconcile heritage protection with rapid development. To understand this tension, we examined a 50.83 km² zone around the Western Han Imperial Mausoleums in the Qin-Han New District. Using Landsat images from 1992, 2002, 2012, and 2022, this study applied supervised classification, land-use transfer matrices, and dynamic-degree analysis to trace three decades of land-use change. From 1992 to 2022, built-up land expanded by 29.85 percentage points, largely replacing farmland, which shrank by 35.64 percentage points and became fragmented. Forest cover gained a modest 5.78 percentage points and migrated eastward toward the mausoleums. Overall, urban growth followed a “spread–integrate–connect” pattern along major roads. This study interprets these trends through five interrelated drivers, including policy, planning, economy, population, and heritage protection, and proposes an integrated management model. The model links archaeological pre-assessment with land-use compatibility zoning and active community participation. Together, these measures offer a practical roadmap for balancing conservation and sustainable land management at imperial burial complexes and similar urban fringe heritage sites. Full article

Climate change alters plant biomass allocation and aboveground–belowground trade-offs in grassland ecosystems, potentially affecting critical functions such as carbon sequestration. However, uncertainties persist regarding how precipitation gradients regulate (1) responses of aboveground biomass (AGB), belowground biomass (BGB), and total biomass in alpine grasslands, and (2) precipitation-mediated AGB-BGB allocation strategies. To address this, we conducted a large-scale field survey across precipitation gradients (400–700 mm/y) in the Sanjiangyuan alpine grasslands, Qinghai–Tibet Plateau. During the 2024 growing season, a total of 63 sites (including 189 plots and 945 quadrats) were sampled along five aridity classes: <400, 400–500, 500–600, 600–700, and >700 mm/y. Our findings revealed precipitation as the dominant driver of biomass dynamics: AGB exhibited equal growth rates relative to BGB within the 600–700 mm/y range, but accelerated under drier/wetter conditions. This suggests preferential allocation to aboveground parts under most precipitation regimes. Precipitation explained 31.71% of AGB–BGB trade-off variance (random forest IncMSE), surpassing contributions from AGB (17.61%), specific leaf area (SLA, 13.87%), and BGB (12.91%). Structural equation modeling confirmed precipitation’s positive effects on SLA (β = 0.28, p < 0.05), AGB (β = 0.53, p < 0.05), and BGB (β = 0.60, p < 0.05), with AGB-mediated cascades (β = 0.33, p < 0.05) dominating trade-off regulation. These results advance our understanding of mechanistic drivers governing allometric AGB–BGB relationships across climatic gradients in alpine ecosystems of the Sanjiangyuan Region on the Qinghai–Tibet Plateau. Full article

Chlorophyll plays a vital role in wheat growth and fertilization management. Accurate and efficient estimation of chlorophyll content is crucial for providing a scientific foundation for precision agricultural management. Unmanned aerial vehicles (UAVs), characterized by high flexibility, spatial resolution, and operational efficiency, have emerged as effective tools for estimating chlorophyll content in wheat. Although multi-source data derived from UAV-based multispectral imagery have shown potential for wheat chlorophyll estimation, the importance of multi-source deep feature fusion has not been adequately addressed. Therefore, this study aims to estimate wheat chlorophyll content by integrating spectral and textural features extracted from UAV multispectral imagery, in conjunction with partial least squares regression (PLSR), random forest regression (RFR), deep neural network (DNN), and a novel multi-source deep feature neural network (MDFNN) proposed in this research. The results demonstrate the following: (1) Except for the RFR model, models based on texture features exhibit superior accuracy compared to those based on spectral features. Furthermore, the estimation accuracy achieved by fusing spectral and texture features is significantly greater than that obtained using a single type of data. (2) The MDFNN proposed in this study outperformed other models in chlorophyll content estimation, with an R² of 0.850, an RMSE of 5.602, and an RRMSE of 15.76%. Compared to the second-best model, the DNN (R² = 0.799, RMSE = 6.479, RRMSE = 18.23%), the MDFNN achieved a 6.4% increase in R², and 13.5% reductions in both RMSE and RRMSE. (3) The MDFNN exhibited strong robustness and adaptability across varying years, wheat varieties, and nitrogen application levels. The findings of this study offer important insights into UAV-based remote sensing applications for estimating wheat chlorophyll under field conditions. Full article

Stem radius growth (GRO), tree water deficit (TWD), and maximum daily shrinkage (MDS) were monitored throughout 2023 in a semi-arid Mediterranean forest stand in Burdur, Türkiye, where Pinus nigra subsp. pallasiana (Lamb.) Holmboe and Pinus brutia Ten. naturally co-occur. These indicators, derived from electronic band dendrometers, were analyzed in relation to key climatic variables. Results indicated that P. brutia had a longer growth period, while P. nigra exhibited a higher average daily increment under the environmental conditions of 2023 at the study site. Annual stem growth was nearly equal for both species. Based on dendrometer observations, P. brutia exhibited lower normalized TWD and higher normalized MDS values under varying vapor pressure deficit (VPD) and soil water potential (SWP) conditions. A linear mixed-effects model further confirmed that P. brutia consistently maintained lower TWD than P. nigra across a wide climatic range, suggesting a comparatively lower degree of drought-induced water stress. GRO was most influenced by air temperature and VPD, and negatively by SWP. TWD was strongly affected by both VPD and SWP, while MDS was primarily linked to minimum air temperature and VPD. Moreover, MDS in P. brutia appeared more sensitive to climate variability compared to P. nigra. Although drought limited stem growth in both species during the study year, the lower TWD and higher MDS observed in P. brutia may indicate distinct physiological strategies for coping with drought. These findings offer preliminary insights into interspecific differences in water regulation under the particular climatic conditions observed during the study year in this semi-arid Mediterranean ecosystem. Full article

Accurately estimating leaves’ relative chlorophyll contents (widely represented by Soil and Plant Analysis Development (SPAD) values) across growth stages is crucial for assessing crop health, particularly in regions characterized by varying sowing dates. Unlike previous studies focusing on high-resolution UAV imagery or specific growth stages, this research incorporates satellite-derived texture indices (TIs) into a SPAD value estimation model applicable across multiple growth stages (from tillering to grain-filling). Field experiments were conducted in Jiangsu Province, China, where winter wheat sowing dates varied significantly from field to field. Sentinel-2 imagery was employed to extract vegetation indices (VIs) and TIs. Following a two-step variable selection method, Random Forest (RF)-LassoCV, five machine learning algorithms were applied to develop estimation models. The newly developed model (SVR-RBF_VIs+TIs) exhibited robust estimation performance (R² = 0.8131, RMSE = 3.2333, RRMSE = 0.0710, and RPD = 2.3424) when validated against independent SPAD value datasets collected from fields with varying sowing dates. Moreover, this optimal model also exhibited a notable level of transferability at another location with different sowing times, wheat varieties, and soil types from the modeling area. In addition, this research revealed that despite the lower resolution of satellite imagery compared to UAV imagery, the incorporation of TIs significantly improved estimation accuracies compared to the sole use of VIs typical in previous studies. Full article

Fractional Vegetation Cover (FVC) is an important parameter to reflect vegetation growth and describe plant canopy structure. This study integrates both active and passive remote sensing, capitalizing on the complementary strengths of optical and radar data, and applies various machine learning algorithms to retrieve FVC. The results demonstrate that, for FVC retrieval, the optimal combination of optical remote sensing bands includes B2 (490 nm), B5 (705 nm), B8 (833 nm), B8A (865 nm), and B12 (2190 nm) from Sentinel-2, achieving an Optimal Index Factor (OIF) of 522.50. The LiDAR data of ICESat-2 imagery is more suitable for extracting FVC than that of GEDI imagery, especially at a height of 1.5 m, and the correlation coefficient with the measured FVC is 0.763. The optimal feature variable combinations for FVC retrieval vary among different vegetation types, including synthetic aperture radar, optical remote sensing, and terrain data. Among the three models tested—multiple linear regression, random forest, and support vector machine—the random forest model outperformed the others, with fitting correlation coefficients all exceeding 0.974 and root mean square errors below 0.084. Adding LiDAR data on the basis of optical remote sensing combined with machine learning can effectively improve the accuracy of remote sensing retrieval of vegetation coverage. Full article

Background/Objectives: The resistance mutations EGFR^{L858R/T790M/C797S} in epidermal growth factor receptor (EGFR) are key factors in the reduced efficacy of Osimertinib. Predicting the inhibitory effects of Osimertinib derivatives against these mutations is crucial for the development of more effective inhibitors. This study aims to predict the inhibitory effects of Osimertinib derivatives against EGFR^{L858R/T790M/C797S} mutations. Methods: Six models were established using heuristic method (HM), random forest (RF), gene expression programming (GEP), gradient boosting decision tree (GBDT), polynomial kernel function support vector machine (SVM), and mixed kernel function SVM (MIX-SVM). The descriptors for these models were selected by the heuristic method or XGBoost. Comprehensive learning particle swarm optimizer was adopted to optimize hyperparameters. Additionally, the internal and external validation were performed by leave-one-out cross-validation ($Q_{LOO}^2$), 5-fold cross validation ($Q_{5-fold}^2$) and concordance correlation coefficient (CCC), $Q_{F1}^2$, and $Q_{F2}^2$. The properties of novel EGFR inhibitors were explored through molecular docking analysis. Results: The model established by MIX-SVM whose kernel function is a convex combination of three regular kernel functions is best: $R^2$ and RMSE for training set and test set are 0.9445, 0.1659 and 0.9490, 0.1814, respectively; $Q_{LOO}^2$, $Q_{5-fold}^2$, CCC, $Q_{F1}^2$, and $Q_{F2}^2$ are 0.9107, 0.8621, 0.9835, 0.9689, and 0.9680. Based on these results, the IC₅₀ values of 162 newly designed compounds were predicted using the HM model, and the top four candidates with the most favorable physicochemical properties were subsequently validated through PEA. Conclusions: The MIX-SVM method will provide useful guidance for the design and screening of novel EGFR^{L858R/T790M/C797S} inhibitors. Full article

Although treeline ecotones are significant components of vulnerable mountain ecosystems and key indicators of climate change, treelines of the Southern Hemisphere remain largely outside of research focus. In this study, we investigate, for the first time, the current and future distribution of the treeline species Nothofagus pumilio in the Southern Andes using a Species Distribution Modelling approach. The lack of modelling studies in this region can be contributed to missing occurrence data for the species. In a preliminary study, both point and raster data were generated using a novel Instagram ground truthing approach and remote sensing. Here we tested the performance of the two datasets: a typical binary species dataset consisting of occurrence points and pseudo-absence points and a continuous dataset where species occurrence was determined by supervised classification. We used a Random Forest (RF) classification and a RF regression approach. RF is applicable for both datasets, has a very good performance, handles multicollinearity and remains largely interpretable. We used bioclimatic variables from CHELSA as predictors. The two models differ in terms of variable importance and spatial prediction. While a temperature variable is the most important variable in the RF classification, the RF regression model was mainly modelled by precipitation variables. Heat deficiency is the most important limiting factor for tree growth at treelines. It is evident, however, that water availability and drought stress will play an increasingly important role for the future competitiveness of treeline species and their distribution. Modelling with binary presence–absence point data in the RF classification model led to an overprediction of the potential distribution of the species in summit regions and in glacier areas, while the RF regression model, trained with continuous raster data, led to a spatial prediction with small-scale details. The time-consuming and costly acquisition of complex species information should be accepted in order to provide better predictions and insights into the potential current and future distribution of a species. Full article

Accurate estimation aboveground biomass (AGB) in winter wheat is crucial for yield assessment but remains challenging to achieve non-destructively. Unmanned aerial vehicle (UAV)-based remote sensing offers a promising solution at the plot level. Traditional field sampling methods, such as random plant selection or full-quadrat harvesting, are labor intensive and may introduce substantial errors compared to the canopy-level estimates obtained from UAV imagery. This study proposes a novel method using Fractional Vegetation Coverage (FVC) to adjust field-sampled AGB to per-plant biomass, enhancing the accuracy of AGB estimation using UAV imagery. Correlation analysis and Variance Inflation Factor (VIF) were employed for feature selection, and estimation models for leaf, spike, stem, and total AGB were constructed using Random Forest (RF), Support Vector Machine (SVM), and Neural Network (NN) models. The aim was to evaluate the performance of multimodal data in estimating winter wheat leaves, spikes, stems, and total AGB. Results demonstrated that (1) FVC-adjusted per-plant biomass significantly improved correlations with most indicators, particularly during the filling stage, when the correlation between leaf biomass and NDVI increased by 56.1%; (2) RF and NN models outperformed SVM, with the optimal accuracies being R² = 0.709, RMSE = 0.114 g for RF, R² = 0.66, RMSE = 0.08 g for NN, and R² = 0.557, RMSE = 0.117 g for SVM. Notably, the RF model achieved the highest prediction accuracy for leaf biomass during the flowering stage (R² = 0.709, RMSE = 0.114); (3) among different water treatments, the R² values of water and drought treatments were higher 0.723 and 0.742, respectively, indicating strong adaptability. This study provides an economically effective method for monitoring winter wheat growth in the field, contributing to improved agricultural productivity and fertilization management. Full article

Traditional methods for predicting feeding amounts rely on historical data and experience but fail to account for non-linear fish growth and the influence of water quality and meteorological factors. This study presents a novel approach for sea bass feeding prediction based on Spearman + RF feature optimization and multi-scale feature fusion using a transformer model. A logistic growth curve model is used to analyze sea bass growth and establish the relationship between biomass and feeding amount. Spearman correlation analysis and random forest optimize the feature set for improved prediction accuracy. A dual-encoder structure incorporates historical feeding data and biomass along with water quality and meteorological information. Multi-scale feature fusion addresses time-scale inconsistencies between input variables The results showed that the MSE and MAE of the improved transformer model for sea bass feeding prediction were 0.42 and 0.31, respectively, which decreased by 43% in MSE and 33% in MAE compared to the traditional transformer model. Full article

Accurate estimation of winter wheat yield under saline stress is crucial for addressing food security challenges and optimizing agricultural management in regional soils. This study proposed a method integrating Sentinel-2 data and field-measured soil salt content (SC) using a random forest (RF) method to improve yield estimation of winter wheat in Kenli County, a typical saline area in China’s Yellow River Delta. First, feature importance analysis of a temporal vegetation index (VI) and salinity index (SI) across all growth periods were achieved to select main parameters. Second, yield models of winter wheat were developed in VI-, SI-, VI + SI-, and VI + SI + SC-based groups. Furthermore, error assessment and spatial yield mapping were analyzed in detail. The results demonstrated that feature importance varied by growth periods. SI dominated in pre-jointing periods, while VI was better in the post-jointing phase. The VI + SI + SC-based model achieved better accuracy (R² = 0.78, RMSE = 720.16 kg/ha) than VI-based (R² = 0.71), SI-based (R² = 0.69), and VI + SI-based (R² = 0.77) models. Error analysis results suggested that the residuals were reduced as the input parameters increased, and the VI + SI + SC-based model showed a good consistency with the field-measured yields. The spatial distribution of winter wheat yield using the VI + SI + SC-based model showed significant differences, and average yields in no, slight, moderate, and severe salinity areas were 7945, 7258, 5217, and 4707 kg/ha, respectively. This study can provide a reference for winter wheat yield estimation and crop production improvement in saline regions. Full article