Search Results (12,863)

Reflection on sustainable economic models, such as the civil economy, has led to the development of alternative food supply chains grounded in ethical values and practices. From this perspective, the Food Village model was proposed to meet stakeholders’ needs, overcome the limitations of Alternative Food Networks, and scale up. In this study, a Discrete Choice Experiment on hypothetical Food Village participation scenarios was combined with the Portrait Values Questionnaire to analyse preferences for the model’s attributes in relation to personal values. The results indicate that consumers appreciate the ethical and territorial characteristics of Food Village, such as local and organic products and cooperative governance, as long as convenience is guaranteed (product variety, flexible hours). Furthermore, they prefer moderate forms of participation, while excessively burdensome involvement reduces their willingness to participate. Individual values influence preferences: values of “self-transcendence” and conservation are associated with greater willingness, while those of “self-affirmation” correlate with lower adherence to Food Village. This evidence suggests implications for policy and scalability: initiatives like Food Village, if supported by public incentives and flexible participatory schemes, can contribute to more sustainable food systems at scale. Full article

Macroalgae plays a significant role in the formulation of innovative and environmentally sustainable approaches to address food challenges. Specifically, green macroalgae serve as dietary supplements aimed at improving the health, growth, and feeding efficiency of various species of marine and freshwater fishes, as well as mollusks. The effects of Chaetomorpha linum extract (CLE) on growth performance, physiological responses, and disease resistance are studied in Bellamya bengalensis against Aeromonas hydrophila. In this experiment, adult B. bengalensis (4412 ± 165.25 mg) were randomly divided into 15 rectangular glass aquariums (35 snail/aquaria; 45 L capacity) and their basal diet was supplemented with different levels of CLE, including 0 (CLE0), 1 (CLE1), 2 (CLE2), 3 (CLE3), and 4 (CLE4) g/kg for 60 days. The growth performance in the CLE3 dietary group was significantly higher that of the CLE0 group, exhibiting both linear and quadratic trends in relation to dietary CLE levels (p < 0.05). The activities of pepsin, amylase, and lipase were found to be highest in CLE3 and lowest in CLE0. Both linear and quadratic responses to dietary CLE levels in digestive enzymes were observed (p < 0.05). The activities of superoxide dismutase and catalase in the hepatopancreas were found to be elevated in snails due to the synergistic effect of the supplemented CLE diet. Among different levels of diet given, CLE2-supplemented snails showed an increase in their enzyme activity (p < 0.05). Interestingly, all the CLE-treated snails expressed elevated levels of mucus lysozyme and mucus protein when compared to control (p < 0.05). Additionally, hepatopancreatic acid phosphatase and alkaline phosphatase activity were elevated in snails consuming CLE3 (p < 0.05). The transcription levels of immune-related genes, including mucin-5ac and cytochrome, were significantly elevated in snails that were fed a diet supplemented with 2–4 g of CLE/kg. Furthermore, the transcription level of the acid phosphatase-like 7 protein gene also increased in snails receiving CLE-supplemented diets. After a 14-day period of infection, snails that consumed a diet supplemented with 3–4 g/kg of CLE exhibited a notable increase in survival rates against virulent A. hydrophila. Based on the above findings, it is suggested that a diet supplemented with 3 g/kg of CLE may enhance growth, antioxidant and immune defense, and disease resistance in the freshwater snail B. bengalensis. Full article

The use of natural bioactive compounds in edible coatings provides a sustainable approach to reducing food spoilage and meeting consumer demand for safer food preservation. In this study, bioactive extracts from Brassica juncea (green mustard, GM) and Raphanus sativus (radish tango, RT) sprouts were encapsulated into zein/chitosan (Z/CH) microparticles (MPs) using a complex coacervation–based encapsulation approach. The encapsulated microparticles (MPs), characterized by FTIR and UV-Vis spectroscopy, demonstrated a high loading efficiency of up to 90% and maintained their antioxidant activity for up to 168 h. TGA and SEM tests confirmed that the edible films produced by incorporating these microparticles (MPs) into polyvinyl alcohol (PVA) and chitosan (CH) matrices had a more uniform microstructure and enhanced heat stability. The Z/CH/RT6:PVA (1:2) and Z/CH/GM6:CH (1:1) formulations of the films showed significant antioxidant and antibacterial action, with up to 22.4% DPPH inhibition and a 1-log decrease in Salmonella enterica CFU, respectively. Overall, the results underscore the promise of sprout-derived microparticles as components for developing active, biodegradable packaging films with improved functional properties. Full article

Background/Objectives: Vending machines in hospitals offer convenient access to snacks and beverages for employees, visitors, and patients. However, their contents are typically energy-dense and nutritionally poor, which can potentially reinforce unhealthy eating habits. This study aimed to evaluate the impact of introducing healthier vending machine options on purchasing behaviour and consumer perceptions in a hospital setting. Methods: An interventional study was conducted at a university clinical centre in Slovenia. Sales data were collected from a standard vending machine and a pilot machine stocked with healthier products over two 14-day periods. Additionally, a consumer survey assessed factors influencing purchasing decisions and opinions on the healthier offerings. Results: The proportion of healthy items purchased increased from 22% to 39% in the pilot vending machine, indicating a positive shift toward healthier choices. However, total sales declined by 18.81%, suggesting consumer hesitation toward the new product mix. Survey results identified price, ingredients, and visual appeal as the primary factors influencing purchase decisions. Conclusions: The introduction of healthier vending machine options can promote better food choices in hospital environments, though challenges remain regarding consumer acceptance and sales performance. Expanding the variety of healthy items and adopting more competitive pricing strategies may enhance uptake. Further long-term research is needed to assess the sustainability of such interventions and their broader impact on hospital food environments. Full article

Salmonella, a major global foodborne pathogen, is a leading cause of salmonellosis. Quantitative detection of Salmonella provides a scientific basis for establishing microbiological criteria and conducting risk assessments. The plate count method remains the primary approach for bacterial quantification, whereas the most probable number (MPN) method is commonly used for detecting low levels of bacterial contamination. However, both methods are time-consuming and labor-intensive. Validated digital polymerase chain reaction (dPCR) techniques are emerging as promising alternatives because they enable rapid, absolute quantification with high specificity and sensitivity. Herein, we developed a novel droplet dPCR (ddPCR) assay for identifying and quantifying Salmonella using invA as the target. The assay demonstrated high specificity and sensitivity, with a limit of quantification of 1.1 × 10² colony-forming units/mL in meat samples. Furthermore, the log₁₀ values obtained via ddPCR and plate counting exhibited a strong linear relationship (R² > 0.99). Mathematical modeling of growth kinetics further confirmed a high correlation between plate count and ddPCR measurements (Pearson correlation coefficient: 0.996; calculated bias factor: 0.88). Collectively, these results indicate that ddPCR is a viable alternative to the MPN method and represents a powerful tool for the quantitative risk assessment of food safety. Full article

Microplastics (MPs) are prevalent in freshwater systems; consequently, fish ingest them either accidentally or intentionally. Once ingested, MPs can translocate to various organs and cause physiological effects. Most studies have focused on tropical and marine fishes, and many have used mass-based methods that measure exposure only by the total mass of microplastics, ignoring particle number and size. These studies have also rarely examined MP effects or fate after a depuration period, limiting our understanding of MP impacts on temperate fishes, hindering the harmonisation of toxicological studies, and complicating assessments of food safety for cultured and wild fish. This study investigated the physiological impacts of dietary exposure to polystyrene microplastics (PS-MPs; 1–10 µm) in Salmo trutta fed a diet with ~5.4 × 10⁶ PS-MPs g⁻¹ feed for 21 days, followed by a 90-day depuration period. PS-MPs translocation from the intestine to the liver and muscle was investigated. Enzymatic biomarkers of oxidative stress and metabolism were analysed in the liver, digestive enzyme activity was assessed in the intestine, and inflammatory enzyme responses were evaluated in both liver and intestinal tissues. In addition, malondialdehyde (MDA) concentration, an indicator of lipid peroxidation, was quantified in blood, muscle, and liver samples. Results show that 1–5 µm PS-MPs translocated to the liver and muscle, while 10 µm particles largely remained in the intestine, with a small fraction detected in muscle tissue but not in the liver. Most biochemical markers were unaffected; however, both trypsin and peroxidase activities significantly decreased after 21 days, and lipid peroxidation increased in blood following 90 days of depuration. PS-MPs persisted in muscle following 90 days of depuration. These findings demonstrate that dietary exposure to PS-MPs in the size range 1–10 µm leads to selective physiological alterations in S. trutta and results in persistent accumulation of MPs in organs, especially muscle tissue consumed by humans, highlighting a clear concern for food safety. Full article

This paper examines whether information-theoretic complexity measures enhance industry-group return forecasting and portfolio construction within a machine-learning framework. Using daily data for 25 U.S. GICS industry groups spanning more than three decades, we augment gradient-boosted decision tree models with Shannon entropy and fuzzy entropy computed from recent return dynamics. Models are estimated at weekly, monthly, and quarterly horizons using a strictly causal rolling-window design and translated into two economically interpretable allocation rules, a maximum-profit strategy and a minimum-risk strategy. Results show that the top performing strategy, the weekly maximum-profit model augmented with Shannon entropy, achieves an accumulated return exceeding 30,000%, substantially outperforming both the baseline model and the fuzzy-entropy variant. On monthly and quarterly horizons, entropy and fuzzy entropy generate smaller but robust improvements by maintaining lower volatility and better downside protection. Industry allocations display stable and economically interpretable patterns, profit-oriented strategies concentrate primarily in cyclical and growth-sensitive industries such as semiconductors, automobiles, technology hardware, banks, and energy, while minimum-risk strategies consistently favor defensive industries including utilities, food, beverage and tobacco, real estate, and consumer staples. Overall, the results demonstrate that entropy-based complexity measures improve both economic performance and interpretability, yielding industry-rotation strategies that are simultaneously more profitable, more stable, and more transparent. Full article

Aspergillus fumigatus, a saprophytic fungus, causes aspergillosis, primarily affecting the immunocompromised. The efficacy of triazole antifungals is compromised by resistance that has developed both clinically and environmentally. Widespread agricultural use of similar triazole fungicides selects for resistant genotypes, leading to potential food contamination and compromising treatment. This study assessed the presence of azole-resistant A. fumigatus in minimally processed food items commonly consumed in Brazil. A total of 25 commercial samples, including black pepper, yerba mate, and green coffee beans, were collected from different regions. Forty-two A. fumigatus isolates were recovered and screened for susceptibility to agricultural and clinical triazoles by determining EC₅₀ values for tebuconazole (0.04–0.7 µg/mL), itraconazole (0.06–0.5 µg/mL), and voriconazole (0.07–0.15 µg/mL). Sequence analysis of the CYP51A gene revealed the presence of M172V mutation, none of which are associated with resistance. Microsatellite genotyping indicated high genotypic diversity and genetic relatedness among isolates from different food sources. Although no azole-resistant phenotypes were identified, the consistent recovery of A. fumigatus from products not directly exposed to azole fungicides highlights the need for continued surveillance. Agricultural environments remain critical hotspots for the emergence and dissemination of resistance, reinforcing the importance of integrated One Health strategies in antifungal resistance monitoring. Full article

This study investigated the optimisation of roselle (Hibiscus sabdariffa L.) leaf tisane formulation using response surface methodology (RSM), targeting total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity as quality indicators. A face-centred central composite design was employed to evaluate dose effects (0.5–2.5 g) and infusion time (5–15 min). Multi-response optimisation using the desirability function identified 1.81 g dose and 5 min infusion as the optimum condition, yielding predicted values of 24.46 mg GAE/100 mL (TPC), 61.07 µmol Fe²⁺/100 mL (FRAP), and 80.47% (DPPH), with a composite desirability score of 0.64. Validation experiments confirmed strong predictive accuracy, with deviations of 0.80% (FRAP) and 3.92% (DPPH), and a modest deviation of 13.2% (TPC), acceptable within complex food matrices. The findings demonstrate that short infusion times are sufficient to extract key bioactives, ensuring consumer convenience and energy efficiency, while valorising roselle leaves as an underutilised by-product into a sustainable functional beverage. Future studies should address sensory acceptance, stability, and bioavailability to support industrial applications further. Full article

Functional food plays an increasingly important role in shaping healthy dietary habits by integrating health-promoting components into consumers’ everyday diets and helping reduce food waste. Products developed using by-products and raw material streams from food processing can support the principles of the circular economy (CE) by improving resource efficiency and lowering the environmental footprint of the food system. In Poland, as in many countries worldwide, functional food is a rapidly growing category that can contribute to public health and serve as a driver of economic development. However, the excessive and scientifically unsupported use of the term “functional food” for marketing purposes may mislead consumers and weaken trust in this product group. From a CE perspective, transparent communication and informed consumer choices are essential, as they promote environmentally responsible behaviors and support sustainable production models. The aim of this study was to assess the level of knowledge about functional food among residents of Eastern Poland, which is a less industrialized and predominantly agricultural region. The analysis focused on awareness of health benefits, consumption frequency, consumer attitudes, and interest in educational initiatives promoting functional food within CE principles. The study also examined whether consumers associate functional food with sustainability-oriented practices. The findings offer insights for educational and marketing strategies grounded in scientific evidence and highlight the potential of functional food in building a sustainable, resource-efficient food system. Full article

Chronic, low-grade inflammation is a characteristic of metabolic diseases like type 2 diabetes. Despite recommendations to select low- or non-fat dairy foods over full-fat dairy foods for metabolic health, recent research suggests potential anti-inflammatory benefits of dairy fat consumption. We aimed to compare the systemic inflammatory tone (i.e., circulating inflammatory biomarker concentrations and ex vivo peripheral blood mononuclear cell inflammatory responses) of individuals with prediabetes after consuming diets with full-fat (3.25%) or non-fat yogurt. We hypothesized that short-term consumption of three daily full-fat yogurt servings beneficially affects inflammatory tone. Thirteen participants aged 45–75 years completed an eight-week randomized, double-masked, controlled crossover study. The two, three-week experimental diets comprised three daily servings of full-fat or non-fat yogurt and were each preceded by a one-week run-in diet. Following each diet, circulating inflammatory biomarkers and cytokine concentrations in the supernatants of peripheral blood mononuclear cells under control or lipopolysaccharide-stimulated conditions were measured. Compared with non-fat yogurt intake, circulating immature granulocyte concentrations were lower following full-fat yogurt intake, but there were no other differences in leukocyte concentrations. Circulating concentrations of cytokines or other inflammatory markers did not differ by diet. Cell supernatant interleukin-1β concentrations were lower following the full-fat yogurt diet under unstimulated conditions but were not different between diets under stimulated conditions. There were no differences by diet in supernatant concentrations of other cytokines under unstimulated or stimulated conditions. Together, minimal differences in inflammatory tone were observed following the short-term consumption of three daily servings of full-fat or non-fat yogurt in individuals with prediabetes. Full article

This article provides a systematic literature review of the scientific literature on forms of communitization that feature alternative nutritional practices to reveal their organizational structures, opportunities, challenges, and transformative potential. The forms studied are alternative food networks and are characterized by their sustainable commitment in food production, distribution, and consumption practices. This review focused solely on articles investigating these forms of communitization in Germany. A systematic literature search was conducted using the databases Web of Science and Business Source Premier in accordance with the PRISMA statement guidelines. Forty-two articles were included in the final analysis, with the oldest article published in 2006 and the newest in 2025. The systematic literature review identifies five forms of communitization with alternative nutritional practices: community, urban and self-harvest gardens; food cooperatives or cooperative initiatives; food sharing and redistribution initiatives; community-supported agriculture and networks; and ecovillages, commune, food initiatives, and other partnerships. The review highlights key forms of communitization that feature alternative nutritional practices, the methods used, and the geographical areas involved. Using content analysis, the organizational structures, opportunities, and challenges of various forms of communitization that feature alternative nutritional practices are identified and their transformative potential discussed. Full article

Determination of the right price is vital for the success of newly developed food products. This study examined the market prices and their determinants for five ready-to-cook catfish products: Panko-Breaded Standard Strips (PBSS), Panko-Breaded Standard Fillet (PBSF), Panko-Breaded Delacata Fillet (PBDF), Sriracha-Marinated Delacata Fillet (SMDF), and Sesame-Ginger-Marinated Delacata Fillet (SGMDF). Market prices were derived using Vickrey’s second-price auction, where the second-highest bid represents the market price. We analyzed experimental auction data from 121 consumers using a logit model to estimate the probability of offering the market price based on product sensory attributes, socio-demographic characteristics of the participants, and the level of competition (panel size). Consumers’ willingness-to-pay (WTP) was elicited in two rounds: before tasting (visual evaluation) and after tasting (organoleptic evaluation) the products. Breaded products received higher market prices than marinated products, with PBDF ranked highest. Sensory traits, especially taste, along with income, education, and grocery shopping involvement, significantly influenced the formation of market price. Increased competition elevated the market prices. Both product features and consumer characteristics significantly affect market price outcomes, and experimental auctions provide a robust tool for understanding consumer behavior toward newly developed food products. Full article

Background/Objectives: To promote sustainable and healthy diets, increased consumption of pulses (the edible grains of legumes) is recommended. Adolescence is a period in which perceptions and behaviours develop that can impact lifelong dietary behaviours. Therefore, this study aimed to understand how Australian adolescents perceive healthy, sustainable diets and perceive and consume pulses. Methods: Students (11–17 years old, median = 15 years, n = 33) in Brisbane, Australia, participated in school-based face-to-face focus groups and an online survey. Results: Students’ perceptions of healthy and sustainable dietary behaviours largely focused on the health aspects of food and consideration of food waste. The main factors that adolescents identified as influencing the health and sustainability of their diet were a lack of perceived responsibility for the impact of their meal choices and lack of knowledge of what constitutes a healthy and sustainable diet. Most students were unfamiliar with the term ‘pulses’ and lacked a desire to consume pulses more often. Consumption of pulses was below recommendations in national and international dietary guidelines. The main factors that adolescents identified as influencing pulse consumption related to students’ lack of capability to prepare pulses and the limited opportunities to access pulse-based foods. Conclusions: Perceptions of sustainable diets and pulses were very limited, and a lack of knowledge, skills, and limited availability were identified as barriers to their consumption. Education and cooking classes, in conjunction with increased availability of tasty pulse foods, are recommended to increase students’ pulse consumption as part of a healthy and sustainable diet. Full article

Background/Objectives: The overconsumption of animal-derived proteins represents a threat to both the environment and our health. Although there is widespread agreement that reducing meat consumption represents a more sustainable alternative, few studies have explored the implicit relations guiding these food choices. This empirical study explores meat consumption and vegetarianism through the lens of Relational Frame Theory. It is hypothesized that people who eat meat have different relational responses to images of meat and plant-based alternatives than vegetarians. Methods: We used the Implicit Attribute Classification Task (IMPACT) to measure relational responses, testing whether omnivores find plant-based proteins (1) less tasty, (2) less familiar, and (3) more expensive than vegetarians do. We registered the response latencies and calculated D-scores from 110 participants who completed an online test. Results: The study failed to find any statistically significant differences in the IMPACT measures between omnivores and vegetarians, given our specific participants and stimuli. Conclusions: Relational responding measures offer a useful approach to understanding consumer choices. However, they are highly sensitive to the task parameters and could be enhanced by further integration with other consumer behavior models when explaining meat consumption. Full article