Search Results (21)

This study evaluated the effectiveness of eDNA metabarcoding in assessing fish communities in two urban lakes (First Lake and Second Lake) in Zagreb, Croatia, following IAS removal. Water samples were collected in April and June 2024 and analyzed using MiFish primers targeting the 12S rRNA gene. The results indicated that the cleanup efforts were largely successful, as several IAS previously recorded in these lakes were not detected (Ameiurus melas, Lepomis gibbosus, and Hypophthalmichthys spp.). However, some others persisted in low relative abundances, such as grass carp (Ctenopharyngodon idella), topmouth gudgeon (Pseudorasbora parva), and prussian/crucian carp (Carassius sp.). Species composition differed between lakes, with common carp (Cyprinus carpio) dominating Maksimir First Lake, while chub (Squalius cephalus) was prevalent in Maksimir Second Lake. Unexpected eDNA signals from salmonid and exotic species suggest potential input from upstream sources, human activity, or the nearby Zoo Garden. These findings underscore the utility of eDNA metabarcoding in biodiversity monitoring and highlight the need for continuous surveillance and adaptive management strategies to ensure long-term IAS control. Full article

Background: Participation in home and wild food procurement (HWFP) activities (i.e., gardening, hunting, fishing, foraging, preserving food, raising livestock, and raising poultry for eggs) skyrocketed during the COVID-19 pandemic. Procuring food through HWFP activities may have important food security and nutrition benefits, while also enhancing food sovereignty and food system resilience. This cross-sectional study examined the effect of HWFP activities on food security status, fruit and vegetable intake, and meat consumption. Methods: We used data collected in 2021 and 2022 from adults (n = 2001) through two statewide representative surveys in Maine and Vermont, United States. Dietary intake was assessed using the Dietary Screener Questionnaire. Food security status was assessed using the U.S. Department of Agriculture’s 6-item short-form food security module. We analyzed the data using linear regression, logistic regression, and ordinal logistic regression models. Results: Sixty-one percent of respondents engaged in HWFP activities; the majority of those gardened. Households engaging in most individual HWFP activities had greater odds of being food insecure. HWFP engagement was positively associated with fruit and vegetable consumption. Specifically, gardening was associated with an additional one cup-equivalent in fruit and vegetable consumption per week compared to respondents that did not garden. Furthermore, when exploring these relationships disaggregated by food security status, we find that this effect is stronger for food insecure households than food secure households. Respondents from households that hunted were more likely to eat wild game meat and also consumed red and white meat more frequently compared to households that did not hunt. Conclusions: Overall, our results indicate potential nutrition and food security benefits from engaging in HWFP activities. Future research should continue to examine a full suite of HWFP activities and their relationship to diet, health, food security, and food sovereignty. Full article

Urbanization and population growth in coastal communities increase demands on local food and water sectors. Due to this, urban communities are reimagining stormwater pond infrastructure, asking whether the stormwater can be used to irrigate food and grow fish for local consumption. Studies exploring this feasibility are limited in the literature. Driven by a community’s desire to co-locate community gardens with stormwater pond spaces, this research monitored the water quality of a 23.4-hectare stormwater pond located in East Tampa, Florida over one year using the grab sample technique and compared the results with U.S. Environmental Protection Agency (EPA) reuse recommendations, EPA national recommended water quality criteria for aquatic life, and human health. pH and conductivity levels were acceptable for irrigating crops. Heavy metal (arsenic, cadmium, copper, lead, and zinc) concentrations were below the maximum recommended reuse levels (100, 10, 200, 5000 and 2000 µg/L, respectively), while zinc and lead were above the criteria for aquatic life (120 and 2.5 µg/L, respectively). E. coli concentrations ranged from 310 to greater than 200,000 MPN/100 mL, above the 0 CFU/100 mL irrigation requirements for raw food consumption and 200 CFU/100 mL requirements for commercial food processing. Synthetic organic compounds also exceeded criteria for human health. Full article

Marine protected areas (MPAs) can allow some fish populations to rebuild within their borders in areas impacted by overfishing, but the effectiveness of reserves is highly dependent on how effectively fishing mortality is controlled, which in turn depends on the level of fishery management implementation. In Cuba’s Gardens of the Queen MPA, the largest in the Caribbean, a variety of fishery management measures have been implemented to ensure the social, economic, and political viability of protecting such a large area. Here, we evaluate the biological response, in terms of fish density and the biomass of commercially valuable and ecologically important reef fish species, to a spatial gradient of fishery management enforcement, in terms of fish density and biomass, of commercially valuable and ecologically important reef fish species. The enforcement gradient is characterized by the level of protection, fishing effort, patrolling effort, distance to the nearest fishing port, and fishing intensity. Fish density and biomass were estimated from visual scuba surveys. Areas with higher levels of enforcement support higher levels of average biomass (up to 1378 kg/ha) and density (up to 2367 indv./ha) of commercially important fishes in comparison to areas with very low or no enforcement (estimates of 757 kg/ha average biomass and 1090 indv./ha average density, respectively). These fish density and biomass levels can serve as proxies in the development of harvest control rules that adjust fishing pressure according to the ratio of fished density or biomass to unfished density or biomass, through the use of the MPA Density Ratio method. Full article

The responses of rare plants to environmental stressors will determine their potential to adapt to a rapidly changing climate. We used a common garden approach to evaluate how six populations of the annual San Diego thornmint (Acanthomintha ilicifolia Lamiaceae; listed as endangered in the state of California and as threatened by the US Fish and Wildlife Service) from across the species range respond in terms of growth (biomass, height, and width) and reproduction (seed production, floral production, and next generation seed viability) to experimental differences in water availability. We found a significant irrigation-by-population interaction on the aboveground growth, wherein the differences in the magnitude and direction of treatment did not correlate directly with climate variables in natural populations. With respect to reproduction, the low-irrigation treatment produced more seeds per plant, more reproductive individuals, and a larger proportion of viable seed in most, but not all, populations. The seed production and the effect of irrigation on seed production correlated positively with rainfall at wild source populations. These results suggest that Acanthomintha ilicifolia responds to water limitation by creating more and higher-quality seed, and that plants locally adapted to a higher annual rainfall show a greater plasticity to differences in water availability than plants adapted to a lower annual rainfall, a finding that can inform the in situ demographic management and ex situ collection strategy for Acanthomintha ilicifolia and other rare California annuals. Full article

Fish Aggregating Devices (FADs) are anchored floating structures often made with cheap scrapped materials and used to aggregate pelagic fish species under their artificial shadows. Globally, the dangerous impact of FADs is well known. They pose a severe threat as a source of bycatch, as a danger to navigation, and with their high potential to become marine litter. Unintended entanglement and consequent mortality in FADs of vulnerable (e.g., sharks, sea turtles, and cetaceans) and commercial species is a serious concern for several international inter-governmental bodies (e.g., EU, GFCM, and IWC). This work describes the first case of a cetacean, a striped dolphin (Stenella coeruleoalba), entangled in a FAD in the Mediterranean Sea. A young male of striped dolphins was found dead along the coast of Lazio (central Tyrrhenian Sea) with its peduncle entangled in typical debris from illegal/artisanal FADs (i.e., a nylon rope, teared gardening plastic sheets, bush branches, and scrapped empty plastic bottles). Although this is the first confirmed case of a cetacean entangled in a FAD in Mediterranean waters, given the extent of the deployment of anchored FADs, the scale of this type of interaction with protected species might be seriously underestimated. Therefore, actions and monitoring need to be implemented urgently to effectively protect and conserve marine biodiversity. Full article

Nontuberculous mycobacteria (NTM) represent an important group of environmentally saprophytic and potentially pathogenic bacteria that can cause serious mycobacterioses in humans and animals. The sources of infections often remain undetected except for soil- or water-borne, water-washed, water-based, or water-related infections caused by groups of the Mycobacterium (M.) avium complex; M. fortuitum; and other NTM species, including M. marinum infection, known as fish tank granuloma, and M. ulcerans infection, which is described as a Buruli ulcer. NTM could be considered as water-borne, air-borne, and soil-borne pathogens (sapronoses). A lot of clinically relevant NTM species could be considered due to the enormity of published data on permanent, periodic, transient, and incidental sapronoses. Interest is currently increasing in mycobacterioses diagnosed in humans and husbandry animals (esp. pigs) caused by NTM species present in peat bogs, potting soil, garden peat, bat and bird guano, and other matrices used as garden fertilizers. NTM are present in dust particles and in water aerosols, which represent certain factors during aerogenous infection in immunosuppressed host organisms during hospitalization, speleotherapy, and leisure activities. For this Special Issue, a collection of articles providing a current view of the research on NTM—including the clinical relevance, therapy, prevention of mycobacterioses, epidemiology, and ecology—are addressed. Full article

Recently, a significant number of freshwater fishing gardens have sprouted up across mainland China. These recreational facilities are an important component in promoting the upgrading of the fishing industry and rural revitalization, and they are a key component in the high-quality development of rural tourism. This paper uses fishing gardens points of interest (POI) in China as data sources and employs kernel density estimation and geographical detectors to systematically uncover the multiscale spatial distribution pattern of these gardens, as well as the factors influencing their distribution. The results show that: (1) There are 15,090 fishing gardens in inland China. The spatial distribution of Chinese fishing gardens corresponds well with the “Hu-Line”, with a greater number of gardens clustered in the southeast and few in the northwest. The density distribution exhibits a polarized pattern with multiple high-density centers. (2) The number of fishing gardens varies significantly across regions, with the eastern > central > western > northeastern; Guangdong has the most fishing gardens. The top five provinces have 43.4% of the total number of fishing gardens in the country. Large-scale fishing gardens are common in developed cities such as the Pearl River Delta, Beijing-Tianjin-Hebei, and the Yangtze River economic belt. (3) In natural environmental factors, land altitude and contour are negatively correlated with the distribution of fishing gardens, whereas winter temperature is positively correlated with the distribution. More than 50% of fishing gardens are located within 6 km of urban built-up areas. (4) GDP, population, and tourism revenue are the most important social development factors influencing the distribution of fishing gardens. The moderate factors are per capita income and the rate of urbanization; the weak factors are fishery output value and freshwater products production. In the discussion, suggestions on how to guide the rational layout and healthy development of the fishing garden industry in the region are put forward. We believe that these suggestions could be part of the pursuit to improve the fishing garden industrial policy in China. Full article

This paper presents the results of the evaluation of the possibility of using a selected sorbent of natural origin, mineral (opoka rock), for the removal of biogenic compounds from aqueous solutions. The analyzed opoka rock contains approximately 70% calcium carbonate (CaCO₃) and from 26 to 27% silicon dioxide (SiO₂) and has been classified as heavy opoka rock. The experiment focused on the sorption of organic components, including phosphorus (P), nitrogen (N) and carbon (C). It was carried out for two treatment systems, column tests and batch tests, for three samples of water—water from a water supply system, water from a fish pond and water from a garden pond—located in the region of Silesia (Poland). The results showed that the P removal efficiency was equal to 96.6% for the fine-grained sorbent (grain size <2 mm) and 90.8% for the coarse-grained sorbent (2–4 mm) in the batch tests, while lower effectiveness was observed for the column tests, reaching 67.8% and 54%, respectively. The efficiency of N (NH₄⁺) removal was equal to 84% for both types of sorbents in the batch tests, while it was 47.7% for the fine-grained sorbent and 26.3% for the coarse-grained sorbent in the column tests. The efficiency of the removal of nitrate-nitrogen (NO₃⁻) was higher for the fine-grained sorbent in all analyzed water samples. The use of materials of natural origin in industrial applications is a recommended direction, part of the green transition. The analyzed samples of opoka rock come from deposits, and opoka rock has so far not been analyzed in terms of its possible use in water and sewage treatment technology. Therefore, further research is recommended for this low-cost sorbent, which may be a competitive material for commercial products. Full article

Chironomids are one of the most biodiverse and abundant members of freshwater ecosystems. They are a food source for many organisms, including fish and water mites. The accurate identification of chironomids is essential for many applications in ecological research, including determining which chironomid species are present in the diets of diverse predators. Larval and adult chironomids from diverse habitats, including lakes, rivers, inland gardens, coastal vegetation, and nearshore habitats of the Great Lakes, were collected from 2012 to 2019. After morphological identification of chironomids, DNA was extracted and cytochrome oxidase I (COI) barcodes were PCR amplified and sequenced. Here we describe an analysis of biodiverse adult and larval chironomids in the Great Lakes region of North America based on new collections to improve chironomid identification by curating a chironomid DNA barcode database, thereby expanding the diversity and taxonomic specificity of DNA reference libraries for the Chironomidae family. In addition to reporting many novel chironomid DNA barcodes, we demonstrate here the use of this chironomid COI barcode database to improve the identification of DNA barcodes of prey in the liquefied diets of water mites. The species identifications of the COI barcodes of chironomids ingested by Lebertia davidcooki and L. quinquemaculosa are more diverse for L. davidcooki and include Parachironomus abortivus, Cryptochironomus ponderosus. Parachironomus tenuicaudatus, Glyptotendipes senilis, Dicrotendipes modestus, Chironomus riparius, Chironomus entis/plumosus, Chironomus maturus, Chironomus crassicaudatus, Endochironomus subtendens, Cricotopus sylvestris, Cricotopus festivellus, Orthocladius obumbratus, Tanypus punctipennis, Rheotanytarsus exiguus gr., and Paratanytarsus nr. bituberculatus. Full article

In 2019, the degree of food self-support in Taiwan was 32.08%, which was lower than that in the previous year by 2.4%. Taiwan does not have the ability to control the availability of food in the face of a food crisis. This study used an aquaponics system to implement the mutualism of fish, flowers, vegetables, and water, as well as to implement the cyclic utilization of water, so that vegetables and fruit could be produced to relieve food shortages in the world. The simulation site of this study was located on a gentle grassy slope alongside Li-tze Lake in Changhua County, Taiwan. In the simulation, three light-tight experimental buckets, with an upper rim diameter of 130 cm, a bottom rim diameter of 125 cm, and a height of 180 cm, were embedded in the ground. The target vegetables and flowers were planted in pots at 17 cm apart, and they were planted on the water surface using the floating island principle. A solar power system enabled a motorized pump to irrigate the plants. An aerator provided adequate aeration to achieve an elementary purifying effect. The experimental results showed that, in terms of the dissolved oxygen, the mean difference of the experimental group was about 1 mg/L, that of the control group was 2 mg/L, and the maximum difference was 6.5 mg/L. As the fish died in April, the ammonia nitrogen value of the control group was 68 times higher than that of the experimental group. Due to the nitrification in July, the ammonia nitrogen decomposed into NO₂, which suddenly increased to 13 mg/L and was extremely unsuitable for the existence of aquatic organisms. This amount was five to six times higher than that of the experimental group. In terms of the fish growth rate, the control group could not bear the drastic changes in the water body at the intermediate stage, and all the fish died, whereas the fish in the experimental group were not affected. The results of this study could provide useful data for gardening, aquatic products, and space design staff. Full article

The study of human–animal interactions has provided insights into the welfare of many species. To date, however, research has largely focused on human relationships with captive mammals, with relatively little exploration of interactions between humans and other vertebrates, despite non-mammals constituting the vast majority of animals currently living under human management. With this study, we aimed to address this gap in knowledge by investigating human–fish interactions at a community garden/aquaponics learning-center that is home to approximately 150 goldfish (Carassius auratus) and seven adult and two juvenile koi (Cyprinus rubrofuscus). After a habituation period (July–September 2019) during which time the fish were regularly provided with the opportunity to engage with the researcher’s submerged hand, but were not forced to interact with the researcher, we collected video data on 10 non-consecutive study days during the month of October. This procedure produced 18~20-min interaction sessions, 10 during T1 (when the experimenter first arrived and the fish had not been fed) and eight during T2 (20–30 min after the fish had been fed to satiation; two sessions of which were lost due equipment malfunction). Interactions between the researcher and the seven adult koi were coded from video based on location (within reach, on the periphery, or out of reach from the researcher) and instances of physical, tactile interaction. Analyses revealed that overall, koi spent more time than expected within reach of the researcher during both T1 (p < 0.02) and T2 (p < 0.03). There were also substantial differences between individuals’ overall propensity for being within-reach and engaging in physical interaction. These results show that koi will voluntarily interact with humans and that individual koi display unique and consistent patterns of interaction. By providing quantitative data to support anecdotal claims that such relationships exist around the world, this research contributes to the ongoing discoveries highlighting the profound dissonance between how humans think about and treat fish and who fish actually are, thereby emphasizing the necessity of stronger moral and legal protections for fishes. Full article

Basil (Ocimum basilicum) was cultivated in northern Germany in three different hydroponic components: grow pipes, a raft, and an ebb-and-flood gravel substrate. The nutrients originated from the intensive production of African catfish (Clarias gariepinus) with 140 fish/m³ under decoupled aquaponic conditions. After 41 days, plants were significantly taller in the gravel components (101.8 ± 8.3 cm), followed by the grow pipes (96.7 ± 7.0 cm), and the raft (94.8 ± 8.6 cm) components (gravel > grow pipes = raft). The leaf number was high and not significantly different between the grow pipes (518.0 ± 81.4), gravel (515.1 ± 133.0), and raft components (493.7 ± 124.8; grow pipes = raft = gravel). Basil in the grow-pipe subsystems developed rapid root growth and clogged the pipes with heterogeneous plant growth. Basil production in northern Germany in grow-pipe, raft, and gravel hydro-components is possible by using effluents from intensive C. gariepinus aquaculture without additional fertilizer in the plant grow-out phase. Further research should focus on optimizing grow pipes by maintaining an optimal root–water contact area, as well as on new technologies such as aquaponics (s.l.) gardening. Full article

The earliest evidence for agriculture in Taiwan dates to about 6000 years BP and indicates that farmer-gardeners from Southeast China migrated across the Taiwan Strait. However, little is known about the adaptive interactions between Taiwanese foragers and Neolithic Chinese farmers during the transition. This paper considers theoretical expectations from human behavioral ecology based models and macroecological patterning from Binford’s hunter-gatherer database to scope the range of responses of native populations to invasive dispersal. Niche variation theory and invasion theory predict that the foraging niche breadths will narrow for native populations and morphologically similar dispersing populations. The encounter contingent prey choice model indicates that groups under resource depression from depleted high-ranked resources will increasingly take low-ranked resources upon encounter. The ideal free distribution with Allee effects categorizes settlement into highly ranked habitats selected on the basis of encounter rates with preferred prey, with niche construction potentially contributing to an upswing in some highly ranked prey species. In coastal plain habitats preferred by farming immigrants, interactions and competition either reduced encounter rates with high ranked prey or were offset by benefits to habitat from the creation of a mosaic of succession ecozones by cultivation. Aquatic-focused foragers were eventually constrained to broaden subsistence by increasing the harvest of low ranked resources, then mobility-compatible Neolithic cultigens were added as a niche-broadening tactic. In locations less suitable for farming, fishing and hunting continued as primary foraging tactics for centuries after Neolithic arrivals. The paper concludes with a set of evidence-based archaeological expectations derived from these models. Full article

Biodiversity is vital for the stability of the planet; its ecosystem services provide essential elements for our survival and well-being. This review analyzes the national biodiversity policies and describes the main strategies for biodiversity conservation in Ecuador, one of the “mega-diverse” countries in the world with the highest species density. It deepens an analysis of in-situ and ex-situ conservation processes. Ecuador has six clear policies for biodiversity conservation. These policies strengthen biodiversity conservation through mechanisms that improve the well-being of wildlife by ensuring human, wildlife and ecosystem health. It promotes actions for the welfare of wildlife, through technical, administrative and legal tools. The National System of Protected Areas, with 60 protected areas, is the most effective in-situ conservation instrument at the country level. Several ex-situ conservation and management means for the conservation of wild species are being utilized, including nurseries, botanical gardens, zoos, germplasm banks, aquariums, species reproduction and rehabilitation centers. Ecuador is making slow progress on ex-situ conservation despite the availability of a sound policy framework, possibly due to financial, infrastructural, and/or technological challenges, and knowledge gaps. We propose fostering international research collaborations and establishing fully funded small-scale captive breeding programs at zoos, aquariums and university research facilities to help recovery of at-risk species of reptiles, amphibians, fish and species beyond Galapagos region. We recommend utilizing citizen science programs to fill the gaps of biodiversity information and increasing efforts to revive the ex-situ conservation strategies in protecting the unique biodiversity of Ecuador. Full article