Search Results (36)

Astaxanthin, a high-value keto-carotenoid with potent antioxidant and health-promoting properties, has gained global attention as a sustainable nutraceutical and biotechnological product. The green microalgae Haematococcus pluvialis and Chromochloris zofingiensis represent two promising natural producers, yet they differ markedly in physiology, productivity, and industrial scalability. This review provides a focused comparative analysis of these two species, emphasizing their quantitative performance differences. H. pluvialis can accumulate astaxanthin up to ~3–5% of dry biomass but typically reaches biomass densities of only 5–10 g L⁻¹, whereas C. zofingiensis achieves ultrahigh biomass concentrations of 100–220 g L⁻¹ under heterotrophic fed-batch fermentation, although its astaxanthin content is much lower (~0.1–0.5% DW). While H. pluvialis remains the benchmark for natural astaxanthin due to its exceptionally high cellular content, its thick cell wall, slow growth, and strict phototrophic requirements impose major cost and operational barriers. In contrast, C. zofingiensis exhibits rapid and flexible growth under heterotrophic, mixotrophic, or phototrophic conditions and can achieve ultrahigh biomass in fermentation, though its ketocarotenoid flux and astaxanthin accumulation remain comparatively limited. Meanwhile, a rapidly growing patent landscape demonstrates global technological competition, with major portfolios emerging in China, the United States, and Europe, spanning chemical synthesis, microbial fermentation, algal metabolic engineering, and high-density cultivation methods. These patents reveal clear innovation trends—ranging from solvent-free green synthesis routes to engineered microalgae and yeast chassis for enhanced astaxanthin production—which increasingly shape industrial development strategies. By synthesizing recent advances in metabolic engineering, two-stage cultivation, and green extraction technologies, this review identifies key knowledge gaps and outlines a practical roadmap for developing next-generation astaxanthin biorefineries, with an emphasis on scalable production and future integration into broader biorefinery frameworks. The findings aim to guide future research and provide actionable insights for scaling sustainable, cost-effective production of natural astaxanthin. Full article

The functional landscape of the skin microbiome is largely defined by dominant genera such as Cutibacterium and Staphylococcus, whereas rare commensals remain poorly understood. In this study, we identified Rothia kristinae BF00107, a skin-resident strain with a complete pantothenate biosynthesis pathway, as a novel postbiotic candidate with distinct dermatological benefits. BF00107 fermentation filtrate suppressed pro-inflammatory cytokines (IL-1β and TNF-α) in keratinocytes and restored extracellular matrix homeostasis in UVB-irradiated fibroblasts by upregulating COL1A1 expression and reducing MMP-1 levels. Consistent with the observed phenotypes, transcriptomic profiling revealed a strain-specific signature characterized by downregulation and upregulation of the expression of inflammatory mediators and barrier- and ECM-associated genes, respectively. Comparative genomics and metabolite profiling confirmed BF00107 as a unique high-pantothenate producer. Supplementation with pantothenic acid reproduced the anti-inflammatory and barrier-supporting effects of the strain, confirming its role as a key effector metabolite. Furthermore, BF00107 passed standard safety assessments, including the Human Repeat Insult Patch Test (HRIPT), Ames, and irritation tests, supporting its suitability for human applications. These findings establish the pantothenate-producing R. kristinae BF00107 as the first functionally validated Rothia strain with anti-inflammatory and photoaging-protective properties. This study expands the functional scope of the skin microbiome and highlights rare commensals as valuable reservoirs for safe, strain-specific postbiotic development. Full article

The Lactobacillaceae family encompasses microorganisms of exceptional ecological and biotechnological importance, serving as central agents in food fermentations, health applications, and nutrient cycling across diverse environments. Despite their broad functional and phylogenetic diversity, the global distribution and ecological specialization of Lactobacillaceae are not yet fully understood. In this study, we performed a comprehensive analysis of over 2 million records from the NCBI database to survey and trace the ecological landscape of Lactobacillaceae across thousands of distinct habitats. Our results reveal that food products and animal hosts represent the primary ecological niches for members of this family. The examined taxa exhibit a broad spectrum of ecological strategies, ranging from generalists with wide environmental adaptability to specialists with strict niche preferences. Notably, our findings highlight a profound geographical and ecological sampling bias, with unclassified taxids frequent in animal gastrointestinal tracts, soils, and especially in living plant tissues—habitats identified as promising frontiers for discovering novel biodiversity. The obtained results emphasize the urgent need for expanded sampling efforts in underexplored geographic regions such as Africa, Antarctica, the Arctic, South America, and Central Asia to capture a more complete picture of Lactobacillaceae diversity. The study underscores the necessity of implementing standardized, metadata-rich data deposition practices to enable unbiased, large-scale ecological and evolutionary analyses. Ultimately, these insights not only deepen our fundamental knowledge of Lactobacillaceae diversity but also provide a strategic framework for future bioprospecting, fostering the discovery of novel strains and expanding the biotechnological potential of this influential bacterial family. Full article

Food waste (FW) presents a critical issue, representing an environmental liability and a largely untapped resource. Its heterogeneity and low valorization rate among main-stream alternative treatments challenge its integration into economically and environmentally sustainable bioprocesses. We explore biorefineries as a solution that can address the complexity of urban food waste through biological strategies capable of converting food waste into valuable products. Exploring the current landscape of FW biorefineries, this study focused on the interplay between feedstock heterogeneity, pretreatment strategies, microbial dynamics, and integration potential. We propose a framework distinguishing between robust fermentations that can use minimally treated FW and tailored fermentations, which require refined media pretreatment and/or supplementation to yield higher-value compounds. Drawing on recent techno-economic and life cycle assessments, this article evaluates process viability and environmental impacts across multiple scales, reinforcing the need for robust analysis to support decision-making. Real-world initiatives and policy frameworks are analyzed to contextualize technological advances within regulatory and infrastructural realities. By linking practical constraints to biochemical and operational strategies, this work outlines how food waste biorefineries can contribute meaningfully to circular economy goals. Instead of treating FW as an intractable problem, it is seen as a versatile feedstock that demands integration, investment, and adaptive process design. Full article

Lignocellulosic agricultural residues represent a rich source of potential feedstock for biorefinery applications, but their valorization remains challenging. The white-rot fungus Irpex lacteus J2 exhibited a promising degradation effect, but its molecular mechanisms of lignocellulose degradation remained largely uncharacterized. Here, we performed high-quality whole-genome sequencing and untargeted metabolomic profiling of I. lacteus J2 during the degradation of corn straw as the sole carbon source. The assembled I. lacteus J2 genome contained 14,647 protein-coding genes, revealing a rich genetic repertoire for biomass degradation and secondary metabolite synthesis. Comparative genomics showed high synteny (mean amino acid sequence identity 92.28%) with I. lacteus Irplac1. Untargeted metabolomic analysis unveiled a dynamic metabolic landscape during corn straw fermentation. Dominant metabolite classes included organic acids and derivatives (27.32%) and lipids and lipid-like molecules (25.40%), as well as heterocyclic compounds (20.41%). KEGG pathway-enrichment analysis highlighted significant activation of core metabolic pathways, with prominent enrichment in global metabolism (160 metabolites), amino acid metabolism (99 metabolites), carbohydrate metabolism (24 metabolites), and lipid metabolism (19 metabolites). Fermentation profiles at 3 and 15 days demonstrated substantial metabolic reprogramming, with up to 210 upregulated and 166 downregulated metabolites. Correlation analyses further revealed complex metabolic interdependencies and potential regulatory roles of key compounds. These integrated multi-omics insights significantly expand our understanding of the genetic basis and metabolic versatility, enabling I. lacteus J2 to efficiently utilize lignocellulose. Our findings position I. lacteus J2 as a robust model strain and provide a valuable foundation for developing advanced fungus-based strategies for sustainable bioprocessing and valorization of agricultural residues. Full article

Population stratification based on gut microbiota composition has revealed several enterotypes in humans and animals, providing valuable tools for studying the gut microbiota landscape, which is crucial for animal health and production. However, knowledge about rumen enterotype identification in sheep, its influencing factors, and its association with growth performance and host genetics remains limited. Here, we investigated host genetic effects and phenotypic landscapes of rumen bacterial enterotypes in a large sheep population. Ruminal contents from 1150 healthy sheep were analyzed using 16S rRNA gene sequencing and genus-level clustering, complemented by extensive phenotypic data covering 47 traits spanning growth, feed efficiency, meat yield, and ruminal fermentation, along with whole-genome resequencing data. We identified two distinct enterotypes: Enterotype 1 (E1), a mixture of multiple genera, and Enterotype 2 (E2), dominated by Prevotella. E2 sheep exhibit superior growth and meat production performance, but lower feed efficiency and increased fat deposition. Two-part beta-regression models and co-occurrence network analyses revealed the extensive impact of enterotypes on microbial community structure, with E1 displaying a higher frequency of unique bacterial interactions. The estimated heritability of the enterotype was 0.47, and a GWAS identified five key genetic markers associated with rumen enterotype, localized to two candidate genes: CHODL and ENPP6. These markers significantly influence 58 ruminal bacterial genera, including key taxa and driving genus. Overall, our data provide new insights into sheep rumen-enterotype characteristics, contributing to a better understanding of microbial interactions that are crucial for improving ruminant growth performance. Full article

The rising demand for sustainable, nutritious, and functional food options has fueled growing interest in plant-based fermented foods. These products offer enhanced sensory, functional, and health-promoting properties, largely driven by microbial activity during fermentation. This review examines recent advances in microbial biotechnology—including the use of novel starter cultures, strain engineering, CRISPR-based genome editing, and precision fermentation that are reshaping the nutritional landscape of plant-based fermented foods. Key benefits such as improved protein digestibility, bioactive compound synthesis, antinutrient reduction, and micronutrient bioavailability are explored. Additionally, the review highlights the potential of microbial innovations to enhance sustainability, address global nutrition challenges, and improve consumer acceptance through better sensory quality. It also discusses challenges related to regulatory frameworks, scalability, and consumer perception. This review aims to provide a comprehensive understanding of how microbial processes can optimize the nutritional and functional value of plant-based fermented foods in alignment with future food system goals. Full article

CO₂ enrichment in protected agriculture is a key strategy for enhancing crop productivity and quality, optimizing photosynthetic efficiency, and mitigating the impacts of climate change. In this study, a comprehensive bibliometric analysis of research on CO₂ enrichment is conducted by compiling and evaluating 171 relevant documents published between 1982 and 2024 in Scopus, utilizing R-Studio and VOSviewer for data processing. The analysis explores scientific output trends, predominant research methodologies, influencing factors, and emerging applications in controlled-environment agriculture. The findings reveal an exponential growth in scientific publications since 2015, with Asia and Europe leading the research landscape. The physiological and agronomic benefits of CO₂ enrichment in C3 crops, particularly tomatoes and lettuce, include enhanced photosynthesis, improved nitrogen assimilation, and reduced abiotic stress. Additionally, advancements in sustainable CO₂ capture and delivery technologies, such as industrial capture and fermentation-based systems, have been documented. However, significant challenges remain regarding the economic feasibility, accessibility for small-scale farmers, and environmental sustainability of CO₂ enrichment strategies. A network analysis of scientific collaboration highlights an increasing trend of international cooperation, with China, the United States, and Japan emerging as key contributors. The integration of plant physiology, agricultural engineering, and environmental sustainability reflects a transition toward multidisciplinary approaches aimed at optimizing CO₂ utilization in controlled environments. This study underscores the potential of CO₂ enrichment as a transformative tool in protected agriculture. However, its large-scale adoption necessitates international collaboration, rigorous research on socio-economic and environmental impacts, and the development of context-specific technologies. Strengthening global research networks and fostering applied innovation will be essential to ensuring the widespread and sustainable implementation of CO₂ enrichment strategies in protected agriculture. Full article

The aim of this review is to provide a comprehensive analysis of the membrane-assisted dark fermentation process for bioH₂ production and purification. This review initially analyses the need for and the current state of the art in H₂ production through dark fermentation, evaluating the research landscape and the maturity level of the technology. Key factors influencing the dark fermentation process are then examined, along with emerging research trends in membrane-assisted fermentative H₂ production systems. This review subsequently addresses the challenges inherent to dark fermentation and explores potential opportunities to enhance H₂ production efficiency. Special attention is given to membrane technology as a promising strategy for process intensification in bioH₂ production and recovery. Finally, this review provides an in-depth discussion of inorganic membranes, mixed matrix membranes (MMMs), and thin and ultrathin membranes, evaluating each membrane type in terms of its advantages, limitations, and purification performance. This review offers valuable insights into intensifying the dark fermentation process by leveraging membrane technology to enhance bioH₂ production and purification efficiency. Full article

Extreme precipitation and flooding frequency associated with global climate change are expected to increase worldwide, with major consequences in floodplains and areas susceptible to flooding. The purpose of this review was to examine the effects of flooding events on changes in soil properties and their consequences on agricultural production. Flooding is caused by natural and anthropogenic factors, and their effects can be amplified by interactions between rainfall and catchments. Flooding impacts soil structure and aggregation by altering the resistance of soil to slaking, which occurs when aggregates are not strong enough to withstand internal stresses caused by rapid water uptake. The disruption of soil aggregates can enhance soil erosion and sediment transport during flooding events and contribute to the sedimentation of water bodies and the degradation of aquatic ecosystems. Total precipitation, flood discharge, and total water are the main factors controlling suspended mineral-associated organic matter, dissolved organic matter, and particulate organic matter loads. Studies conducted in paddy rice cultivation show that flooded and reduced conditions neutralize soil pH but changes in pH are reversible upon draining the soil. In flooded soil, changes in nitrogen cycling are linked to decreases in oxygen, the accumulation of ammonium, and the volatilization of ammonia. Ammonium is the primary form of dissolved inorganic nitrogen in sediment porewaters. In floodplains, nitrate removal can be enhanced by high denitrification when intermittent flooding provides the necessary anaerobic conditions. In flooded soils, the reductive dissolution of minerals can release phosphorus (P) into the soil solution. Phosphorus can be mobilized during flood events, leading to increased availability during the first weeks of waterlogging, but this availability generally decreases with time. Rainstorms can promote the subsurface transport of P-enriched soil particles, and colloidal P can account for up to 64% of total P in tile drainage water. Anaerobic microorganisms prevailing in flooded soil utilize alternate electron acceptors, such as nitrate, sulfate, and carbon dioxide, for energy production and organic matter decomposition. Anaerobic metabolism leads to the production of fermentation by-products, such as organic acids, methane, and hydrogen sulfide, influencing soil pH, redox potential, and nutrient availability. Soil enzyme activity and the presence of various microbial groups, including Gram+ and Gram− bacteria and mycorrhizal fungi, are affected by flooding. Waterlogging decreases the activity of β-glucosidase and acid phosphomonoesterase but increases N-acetyl-β-glucosaminidase in soil. Since these enzymes control the hydrolysis of cellulose, phosphomonoesters, and chitin, soil moisture content can impact the direction and magnitude of nutrient release and availability. The supply of oxygen to submerged plants is limited because its diffusion in water is extremely low, and this impacts mitochondrial respiration in flooded plant tissues. Fermentation is the only viable pathway for energy production in flooded plants, which, under prolonged waterlogging conditions, is inefficient and results in plant death. Seed germination is also impaired under flooding stress due to decreased sugar and phytohormone biosynthesis. The sensitivity of different crops to waterlogging varies significantly across growth stages. Mitigation and adaptation strategies, essential to the management of flooding impacts on agriculture, enhance resilience to climate change through improved drainage and water management practices, soil amendments and rehabilitation techniques, best management practices, such as zero tillage and cover crops, and the development of flood-tolerant crop varieties. Technological advances play a crucial role in assessing flooding dynamics and impacts on crop production in agricultural landscapes. This review embarks on a comprehensive journey through existing research to unravel the intricate interplay between flooding events, agricultural soil, crop production, and the environment. We also synthesize available knowledge to address critical gaps in understanding, identify methodological challenges, and propose future research directions. Full article

Red dragon fruit (Hylocereus polyrhizus), recognized globally for its substantial nutrient content and health benefits, has been extensively studied; studies have particularly focused on the fruit, while the composition of the stem remains less explored. This research focuses on optimizing fermentation parameters for red dragon fruit wine, specifically examining yeast-strain selection, juice-to-water dilution ratios, and yeast concentrations. Saccharomyces cerevisiae RV002 emerged as the optimal strain due to its robust performance and adaptability under adverse conditions. The study identified a 50% dilution ratio as ideal for maximizing clarity and the sensory attributes of the wine, whereas dilution ratios exceeding 90% significantly reduced ethanol content below acceptable commercial standards. An optimal yeast concentration of 1 g/L was found to balance microbial suppression and alcohol yield effectively; deviations from this concentration led to microbial contamination or impaired fermentation dynamics. Fermentation markedly altered the biochemical properties of Hylocereus polyrhizus, reducing sugar and vitamin C levels while increasing polyphenol content and antioxidant activity, thereby enhancing potential health benefits. These findings underscore the transformative effects of microbial activity on the substrate’s chemical landscape and highlight the potential of tailored fermentation strategies to enhance the utility and value of underutilized fruits in sustainable agricultural practices. Full article

This review aims to create a communication tool for low-alcohol and nonalcoholic wine production, catering to scientists, educators, students, and wine producers in the field. With health concerns regarding alcohol consumption, the need for information on low-alcohol wines is essential. This paper outlines the methods for the pre-fermentation (leaf area reduction, early grape harvest, grape must dilution, filtration of grape juice and addition of glucose oxidase), mid-fermentation (employing non-saccharomyces yeasts, using genetically modified yeasts through metabolic engineering, and controlling yeast nutrition), and post-fermentation (nanofiltration and reverse osmosis, osmotic distillation, pervaporation, spinning cone column, vacuum distillation, and multi-stage membrane-based systems) stages and their effects on wine quality. It also presents evidence of the impact of alcoholic, low-alcohol, and nonalcoholic wines on cardiovascular health. Finally, the potential market for low-alcohol and nonalcoholic wines is discussed. Key findings indicate a shift toward low-alcohol alternatives due to health, economic, and social factors and consumer interest in healthier lifestyles. Low-alcohol and nonalcoholic wines offer health benefits, particularly cardiovascular health, presenting an opportunity for winemakers to cater to a health-conscious market. From an economic perspective, the low-alcohol and nonalcoholic wine market is poised to grow and diversify its revenue streams. The development of high-quality low-alcohol and nonalcoholic wines, which can command premium prices, enhances profitability. The changing regulatory landscape in Europe, with a focus on transparency in alcohol labeling and nutritional information, aligns with the new consumer preferences and regulatory standards. Full article

The sustainable production of natural compounds is increasingly important in today’s industrial landscape. This study investigates the metabolic engineering of Saccharomyces cerevisiae for the efficient biosynthesis of valuable carotenoids: canthaxanthin, zeaxanthin, and astaxanthin. Utilizing a tailored parental yeast strain, Sp_Bc, we optimized the carotenoid pathway by screening and identifying CrtW and CrtZ enzymatic variants. The CrtW variant from Bradyrhizobium sp. achieved a canthaxanthin titer of 425.1 ± 69.1 µg/L, while the CrtZ variant from Pantoea ananatis achieved a zeaxanthin titer of 70.5 ± 10.8 µg/L. Additionally, we optimized carotenoid production by exploring enzyme fusion strategies for all three studied carotenoids and organelle compartmentalization specifically for enhancing astaxanthin synthesis. We further improved carotenoid production by integrating the optimal gene constructs into the yeast genome and deleting the GAL80 gene, enabling the use of sucrose as a carbon source. The engineered strain Sp_Bc-Can001 ∆gal80 was evaluated in a 5 L bioreactor fermentation, achieving a notable canthaxanthin titer of 60.36 ± 1.51 mg/L using sucrose. This research conclusively establishes S. cerevisiae as a viable platform for efficient carotenoid biosynthesis and, for the first time in this yeast system, illustrates sucrose’s viability as a carbon source for canthaxanthin production. These findings pave the way for sustainable, cost-effective carotenoid production at an industrial scale. Full article

It is widely known that mushrooms present several properties with applications in the medicinal and pharmaceutical sectors, including antimicrobial, immunomodulatory, antioxidant, hypotensive, neuroprotective, and anti-inflammatory activities. This article aims to review examples of the bioactive metabolites responsible for those activities, such as polysaccharides, phenols and polyphenols, terpenes, peptides, alkaloids, and steroids, which are produced by several relevant mushroom species. It also discusses their production through solid-state fermentation and submerged fermentation, as well as the processes of obtention of mushroom bioactive extracts and considerations on their stability aiming industrial applications. In addition, the article examines the patent landscape surrounding mushroom-derived bioactives, shedding light on the intellectual property history and innovations driving this field forward. Examples of recently deposited patents in the field are highlighted, as well as the main depositors. China and the United States are the major depositor countries in this field (52% and 35% of patents, respectively), and the principal compounds on the patents are polysaccharides and alkaloids. The article also provides insights into the current market landscape, showcasing mushroom-derived products in the pharmaceutical field available to consumers. From dietary supplements to skincare formulations, the market offerings reflect the growing interest in harnessing the health benefits of mushroom bioactives. Full article

The low FODMAP (fermentable oligosaccharide, disaccharide, monosaccharide, and polyol) diet is a beneficial therapeutic approach for patients with irritable bowel syndrome (IBS). However, how the low FODMAP diet works is still not completely understood. These mechanisms encompass not only traditionally known factors such as luminal distension induced by gas and water but also recent evidence on the role of FOMAPs in the modulation of visceral hypersensitivity, increases in intestinal permeability, the induction of microbiota changes, and the production of short-chain fatty acids (SCFAs), as well as metabolomics and alterations in motility. Although most of the supporting evidence is of low quality, recent trials have confirmed its effectiveness, even though the majority of the evidence pertains only to the restriction phase and its effectiveness in relieving abdominal bloating and pain. This review examines potential pathophysiological mechanisms and provides an overview of the existing evidence on the effectiveness of the low FODMAP diet across various IBS subtypes. Key considerations for its use include the challenges and disadvantages associated with its practical implementation, including the need for professional guidance, variations in individual responses, concerns related to microbiota, nutritional deficiencies, the development of constipation, the necessity of excluding an eating disorder before commencing the diet, and the scarcity of long-term data. Despite its recognized efficacy in symptom management, acknowledging these limitations becomes imperative for a nuanced comprehension of the role of a low FODMAP diet in managing IBS. By investigating its potential mechanisms and evidence across IBS subtypes and addressing emerging modulations alongside limitations, this review aims to serve as a valuable resource for healthcare practitioners, researchers, and patients navigating the intricate landscape of IBS. Full article