Search Results (92)

To investigate the impacts of the continuous application of urban sewage sludge on heavy metal pollution risks in wine grape orchards, this study conducted a five-year field plot experiment using wine grapes as the test crop. The experimental design included three sludge application rates and a control without sludge application. Soil physicochemical properties, the single-factor and integrated pollution indices (PI and NIPI) of heavy metals, potential ecological risk indices (EI and RI), and the safe application duration of sludge were analyzed. The results suggest that sludge application significantly increased soil organic matter, total nitrogen, total phosphorus, and available phosphorus by 39.99–46.56%, 59.37–73.69%, 83.57–143.19%, and 88.79%, respectively, while reducing soil bulk density by 8.70–27.92%. The PI and EI of Cd exhibited significant linear increases with the duration of sludge application, with annual increments of 0.010 and 0.31, respectively. Hg was influenced by both the application rates and duration, with annual increments of 0.013 and 0.52 for the PI and EI, respectively. These two elements collectively drove overall increases of 7.31–24.96% in NIPI and 32.51–59.90% in RI, with mean annual increases of 0.0064 and 0.84, respectively. In contrast, Cr, Pb, and As showed no significant changes. Based on the calculated environmental capacities of Cd and Hg, the safe application durations were estimated to be 46.99–126.93 and 48.58–131.21 years, respectively. These results demonstrate that under the current application intensity, sludge can improve soil fertility in the short term with controllable ecological risks. However, considering their potential environmental risks, the continuous accumulation of Cd and Hg necessitates vigilance. Full article

The transition toward sustainable and circular agricultural systems is increasingly important, yet evidence linking circularity and farm profitability in transition economies remains limited. This study examines the determinants of farm profitability in Serbia by combining micro-level structural and productivity indicators with a macro-level agro-eco efficiency measure, used here as a sector-wide ecological pressure indicator rather than a direct proxy for circular practices. Using a balanced Farm Accountancy Data Network (FADN) panel of 443 farms (2015–2022) across dairy, mixed, field crop, and fruit & wine sectors, dynamic panel estimators (difference and system Generalized Method of Moments-GMM) reveal strong sectoral heterogeneity. Asset turnover is the primary driver of profitability in field crops and perennial systems, while dairy farms benefit from scale and land productivity. Energy intensity consistently reduces profitability across all sectors. Agro-eco efficiency shows a negative effect in livestock-based systems, indicating higher sensitivity to macro-ecological pressures. These findings suggest that environmental and economic vulnerabilities differ across production systems, highlighting the need for sector-specific strategies aimed at improving resilience rather than inferring the profitability of circular technologies. Full article

Grape harvest time influences the berry composition, which impacts the organoleptic wine characteristics. Winemakers monitor technological, phenolic, and aromatic grape maturity to decide the harvest date. Little is known about the evolution of yeast ecology of grapes during the maturation period. The microbiota involved in the fermentation impacts the wine composition and characteristics; therefore, changes in grape biodiversity could have an impact in fermentation kinetics and aroma compound formation. In this study, the yeast biodiversity of Grenache Noir and Carignan grapes from Montsant DO (Denomination of Origin) were analyzed at different ripening stages to assess how harvest time influences microbiota. The fermentation performance of the yeasts obtained was studied at both laboratory and pilot scales to evaluate the impact of these yeasts, both in pure and mixed cultures, on the wine’s chemical and aromatic composition as well as its sensory impact. The results indicated that early harvest favored a higher diversity of non-Saccharomyces species, whereas in more mature grapes, Saccharomyces cerevisiae species was dominant. The isolated strains of Saccharomyces differed in their fermentation performances, as well as ethanol content and acidity of wine. In general, they produced higher concentration of fermentative volatile compounds than a commercial strain. The non-Saccharomyces yeasts in mixed fermentations with the S. cerevisiae strains also impacted wine composition and characteristics, leading lower ethanol content or enhancing aromatic balance and sensory equilibrium. The results highlight that grape harvest timing influences microbial diversity and fermentative performance and thus should be considered to better determine the optimum harvest date and ensure consistent wine characteristics. Full article

The implementation of vegetative cover crops in vineyards is a sustainable alternative to chemical weed control, potentially influencing both soil fertility and grape-associated microbiota. This study evaluated the impact of six groundcover management strategies under vines—white clover (Trifolium repens), red clover (Trifolium pratense), burr medic (Medicago polymorpha), lupine (Lupinus albus), spontaneous weeds, and an herbicide-treated control—on the microbial dynamics and physicochemical properties of Cabernet Sauvignon must and wine from the Maipo Valley, Chile. Amplicon sequencing of bacterial (16S rRNA) and fungal (ITS) communities was combined with spontaneous fermentation trials and chemical analyses of must and wine. Fungal and bacterial communities on grape surfaces were dominated by Ascomycota and Proteobacteria, respectively, with no significant compositional differences among treatments. During fermentation, Metschnikowia and Tatumella were the most abundant non-Saccharomyces and bacterial genera, respectively, showing dynamic shifts across fermentation stages. Legume-based covers, particularly red clover, increased wine total acidity and polyphenol index while reducing pH. Correlation analyses revealed associations between specific microbial taxa (Metschnikowia, Cohnella, Saliterribacillus) and key enological parameters. Overall, these findings demonstrate that leguminous cover crops subtly modulate vineyard microbial ecology and fermentation outcomes, offering an environmentally sustainable pathway to enhance enological differentiation in semi-arid viticultural regions. Full article

The Criaderas and Solera system represents one of the most sophisticated aging methodologies in winemaking, producing distinctive Sherry wines through dynamic blending and biological aging processes. This traditional Spanish system, combined with the unique metabolic activities of flor yeast (Saccharomyces cerevisiae), creates wines of exceptional complexity and consistency. This comprehensive review synthesizes current literature on the Criaderas and Solera system, focusing on biological aging mechanisms, flor yeast dynamics, industrial applications, and contemporary challenges. Recent advances in genomic, proteomic and metabolomic studies of flor yeasts have been analyzed, alongside modern analytical approaches to the characterization of Sherry wine. The Criaderas and Solera system enables consistent wine quality through fractional blending across multiple aging levels. Flor yeasts exhibit specialized metabolic adaptations, including enhanced ethanol oxidation capacity, biofilm formation abilities, and stress resistance mechanisms. Modern applications extend beyond traditional winemaking to include biotechnological uses, immobilization techniques, and sustainable production methods. Current challenges include climate change impacts, maintaining genetic diversity, adapting to new technologies and meeting evolving consumer preferences. The integration of traditional knowledge with modern biotechnology offers promising opportunities for optimizing Sherry production. Understanding flor yeast ecology and metabolic pathways provides insights for developing innovative applications while preserving the authenticity of this centuries-old winemaking tradition. Full article

As a wine-producing region in China, Xinjiang’s ecological conditions endow grapes with distinctive flavor potential. However, systematic research on volatile compounds in wines from Vitis amurensis Rupr. varieties in this region remains limited. Therefore, wines from four Xinjiang Vitis amurensis varieties (‘Shuanghong’, ‘Zuoyouhong’, ‘Xuelanhong’, and ‘Beibinghong’) were analyzed using high-performance liquid chromatography (HPLC), headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS), electronic nose (E-nose), odor activity value (OAV) calculation, and multivariate analysis. Physicochemical parameters, organic acids, volatile organic compounds (VOCs), and OAVs were determined. Results showed significant differences in physicochemical properties among the varieties, potentially correlating with wine mouthfeel. Beibinghong wine contained the highest total VOC concentration. Among 64 identified VOCs, 37 had OAVs ≥ 1. Multivariate analysis identified 14 key differential volatile compounds (VIP ≥ 1, p < 0.05) responsible for flavor differences between varieties, with each variety exhibiting distinct key compounds. E-nose analysis effectively distinguished the aroma profiles of the four wines. This study elucidates the chemical and volatile compound characteristics of wines from Xinjiang Vitis amurensis varieties, providing a theoretical foundation for research on their flavor profiles. It also aids in selecting Vitis amurensis varieties for cultivation and supports the development of distinctive regional wines in Xinjiang. Full article

Microbial inoculants, as a new type of product that combines economic efficiency with ecological sustainability, play an important role in promoting plant growth and development, increasing crop yields, and enhancing plant resistance to abiotic stress. This study used the wine grape cultivar (Vitis vinifera ‘Pinot Noir’) as experimental material to systematically investigate the effects of microbial inoculants on the soil–leaf–fruit system during the late growth stage of grapes under salt stress conditions (200 mM NaCl). This study analyzed the regulatory effects of microbial inoculants on soil physicochemical properties, leaf physiological and biochemical characteristics, as well as fruit yield and quality. The results showed that salt stress significantly inhibited the growth of Pinot Noir grapes. However, the application of microbial inoculants effectively alleviated the negative effects of salt stress. By enhancing the plant’s antioxidant defense capacity and regulating physiological metabolic pathways such as osmotic balance, the inoculants significantly mitigated the inhibitory effect of salt stress on fruit development. Notably, the S+JH treatment group demonstrated particularly outstanding results, with hundred-berry weight, single-bunch weight, and yield per plant increasing significantly by 15.96%, 12.47%, and 28.93%, respectively, compared to the salt stress group (S). Additionally, this treatment also stabilized free amino acid content and suppressed excessive organic acid synthesis. This study provides new technical insights into the application of microbial inoculants for saline-alkali land improvement and stress-resistant cultivation of horticultural crops such as grapes, holding significant practical value for promoting the sustainable development of the grape industry in saline-alkali regions. Full article

The wine sector faces urgent challenges related to climate change, resource constraints, rising energy costs, and shifting consumption patterns. In this context, sustainability represents a lever of competitiveness and resilience, enabling producers to differentiate themselves through their connection with the territory and the preservation of artisanal practices. This is particularly evident for native varieties, such as the Apulian Primitivo, whose market repositioning is based on production choices that combine sustainability and craftsmanship, generating wines with distinctive organoleptic characteristics while also contributing to the environmental, economic, and social protection of the production areas. At the same time, the spread of e-commerce offers new opportunities to enhance these characteristics: online descriptions enrich the communication of details compared to the physical label, reducing information asymmetries and making signs of quality and sustainability more visible. Therefore, this study applies a hedonic model to estimate the implicit price of such practices, analyzing 656 Primitivo labels through simultaneous quantile regressions. The results show that sensory characteristics, such as alcohol content and ageing, determine the price throughout distribution, while identity and sustainable attributes have heterogeneous effects: ecological practices and Geographical Indications affect the lower quantiles, while indigenous yeasts and sapling vines are configured as premium signals in the upper segments. Full article

Toxicological studies of pesticides in animal models provide critical insights into their mechanisms of action, while adsorption strategies offer potential solutions for decontaminating polluted waters. We evaluated toxicity induced by tetraethyl pyrophosphate (TEPP), an organophosphate pesticide and AChE inhibitor, on zebrafish (Danio rerio) development and behavior, alongside the efficacy of wine cork granules as a natural adsorbent. TEPP exposure reduced embryo viability following an inverted U-shaped dose–response curve, suggesting non-monotonic neurodevelopmental effects, but did not alter developmental timing or morphology in survivors. In juveniles, TEPP increased preference for dark environments (33% vs. controls) and enhanced swimming endurance approximately 3-fold, indicating disrupted phototaxis and stress responses. Most strikingly, water treated with cork granules retained toxicity, increasing mortality, delaying embryogenesis, and altering behavior. This directly contradicts in vitro adsorption studies that suggested cork’s efficacy. These results demonstrate the high sensitivity of zebrafish to TEPP at nanomolar concentrations, which contrasts with in vitro models that require doses approximately 1000 times higher. Our findings not only highlight TEPP’s ecological risks but also reveal unexpected limitations of cork granules for environmental remediation, urging caution in their application. Full article

In the present paper, extracts from pomace after cherry wine production were used as biocomponents of antioxidant packages. In the study, the highest concentrations of polyphenolic compounds were obtained when a 50% ethanol solution was used as the extraction solution. The addition of extracts provided statistically significant (p < 0.05) changes in water vapor transmission for the films obtained. The WVTR results are at a very low level, as values ranging from 7.96 ± 0.33 [g/m² d] (sample 2) to 10.95 ± 0.33 [g/m² d] (sample 1) were obtained. The addition of extract also affected the oxygen barrier. Samples without extract addition showed an OTR value of 2.42 ± 0.23 [cm³/m² d 0.1 MPa]. A statistically significant (p < 0.05) reduction in this parameter was affected by the addition of extract to the matrix. Oxygen barrier properties ranged from 0.50 ± 0.05 (sample 3) to 0.94 ± 0.04 (sample 1), indicating high barrier properties of the packaging material. The addition of extracts caused an increase in opacity: films 3 and 4 were characterized by the highest value of the parameter, which was, respectively: 18.14 ± 27.02 and 18.97 ± 29.83 [%]. The research carried out in this study allows us to conclude that bioactive films with high application potential have been achieved and, in addition, represent a natural and ecological alternative to the materials currently used. Full article

Italy is a global top wine producer, with emphasis on high-quality wines. This study investigates the Carbon Footprint (CF), Water Footprint (WF), and Ecological Footprint (EF) of twelve red wine producers in Piedmont, Northern Italy. The analysis was based on a 0.75 L wine bottle as functional unit (FU). Twelve producers were interviewed and given questionnaires, which made it possible to gather primary data for the environmental evaluation that described vineyard and agricultural operations and wine production. The average CF was 0.88 ± 0.3 kg ${CO}_{2eq},$ with 44% of CF associated with the glass bottle, 20% to the diesel fuel fed to the agricultural machines, 32% to electricity consumption, and 4% to other contributions. The average WF was 881 ± 252.4 L, with 98% Green WF due to evapotranspiration, and 2% Blue and Grey WF. The average EF was 81.3 ± 57.2 global ha, 73% ascribed to the vineyard area and 27% to CO₂ assimilation. The obtained CF and WF values align with existing literature, while no comparison is possible for the EF data, which are previously unknown. To reduce the environmental impacts of wine production, actions like using recycled glass bottles, electric agricultural machines and renewable energy can help. However, high-quality wine production in Piedmont is deeply rooted in tradition and mostly managed by small producers. Further research should investigate the social acceptance of such actions, and policies supporting economic incentives could be key enablers. Full article

Vin Santo is a passito wine produced mainly in Tuscany. In the traditional production of Vin Santo, fermentation occurs naturally. Only a few reports have explored the microbial ecology of Vin Santo. Therefore, the present study aimed to investigate the microbial ecology and its impact on the fermentative kinetics in traditional processes of Vin Santo carried out in two different Tuscan wineries. Despite the different systems used for drying the grapes, both wineries showed similar microbial ecology. Non-Saccharomyces yeasts were the dominant microbial population during grape drying in different succession, even though in the end, the dominant species (at different percentages) in both were Metschnikowia pulcherrima, Kloeckera apiculata, and Starmerella bacillaris. The spontaneous fermentations were instead both dominated by Saccharomyces cerevisiae, however in different concentration throughout the process, leading to a different ethanol content—12% (v/v) and 10.8% (v/v) in winery A and B, respectively. In both wineries, acetic bacteria and moulds did not grow. Considering the intraspecific biodiversity of S. cerevisiae populations, the vinifications of both wineries displayed very similar biodiversity indices. No single strain of S. cerevisiae dominated the entire fermentation process. The analysis identified 30 distinct genetic patterns in the fermentations of winery A and 23 in the fermentations of winery B. The work provided an insight into the microbial communities and their metabolomic interactions during Vin Santo production which could improve the management and control of the process. Full article

Water pollution by antibiotics and heavy metals threatens the ecological environment and human health globally, yet there is no rapid method to detect multiple antibiotics and metal ions simultaneously. A simple, fast, and ultra-sensitive colorimetric chemosensor for the simultaneous detection of moxifloxacin (MOX), ciprofloxacin (CIP), and Cr(III) based on the aggregation of ammonium thioglycolate (ATG)-functionalized gold nanoparticles (ATG-AuNPs) was developed. Following the addition of MOX, CIP, and Cr(III), a color change in the solution was observed from wine-red to blue-grey. The UV–Vis signal of the ATG-AuNPs system blended with MOX, CIP, and Cr(III) in the range of 0~200 µM, 0~100 µM, and 0~5 µM was assessed and measured with detection limits (LODs) of 1.57 µM, 1.30 µM, and 57.1 nM calculated by 3σ/S, respectively. Therefore, this system has the potential to act as an effective colorimetric chemosensor for simultaneously detecting MOX, CIP, and Cr(III) in complex environmental systems. Full article

Our study was based on the premise that the type of soil tillage and the kind of fertilization significantly affect soil properties, nutrient availability, and uptake by Vitis vinifera L. (cv. ‘Robola’) plants. For this purpose, a two-year field experiment was conducted, in a 2 × 3 factorial (i.e., two types of soil tillage-conventional and reduced and three kinds of fertilization-conventional, controlled N release and organic), with six treatments derived from the combination of the two tillage and the three fertilization methods. The results showed that the organic matter content (%), as well as the exchangeable Mg, were significantly influenced by the type of tillage. The kind of fertilization affected soil nitrate and leaf N (lower values in the organic fertilization) and P concentrations (higher values in the organic fertilization). Regarding the effect of the type of tillage, foliar Mg was significantly higher in the conventional soil tillage. Finally, both the type of tillage and kind of fertilization significantly affected leaf Zn. Overall, these data show the importance of innovative dual co-application of pomace (an organic by-product of the wine industry) with reduced soil tillage on soil properties and plant nutrition. Thus, it is expected to gain environmental, ecological, and economic benefits for wine producers and also to improve vineyards’ sustainability and protected designation of origin (PDO) wine quality under the challenges provoked by climatic and recent energy crises. Full article

The invasive spotted wing Drosophila (SWD) represents new challenges for European and North American fruit producers. The aim of our study was to examine wine grape cultivar susceptibility to this pest and melanogaster-type Drosophila (MTD) by surveying drosophilid populations using field traps and conducting emergence tests. We assessed fly development from intact and artificially injured berries collected from four cultivars. Berries were incubated individually and in pooled samples to evaluate infestation patterns and potential larval interactions. Although grapes are generally considered less favorable hosts for SWD, the pest was consistently present across all vineyard plots. Infestation levels differed significantly among cultivars, with the Hungarian white cultivar Furmint being the most susceptible, while French-origin red cultivars Cabernet Franc and Cabernet Sauvignon, along with the other Hungarian cultivar Rózsakő, were less susceptible. Berry integrity played a crucial role: intact berries showed minimal infestation, whereas physical injuries led to a substantial and significant increase in infestation rates and fly emergence. In contrast to SWD-dominated trap catches and the nearly equal proportions of SWD and MTD observed in intact berries, injured berries were predominantly colonized by MTD. This dominance became even more pronounced in pooled samples, suggesting that larval competition in shared environments favors MTD over SWD. These findings underscore the importance of grape cultivar traits and berry condition in shaping Drosophila infestation dynamics. Further research into the chemical and ecological drivers of host selection and interspecific interactions is warranted to improve vineyard pest management strategies. Full article