Search Results (139)

Background/Objectives: Tau protein, a central player in Alzheimer’s disease (AD) pathology, is classically known for its role in microtubule stabilisation. However, accumulating evidence indicates that tau also localises to the neuronal nucleus, particularly the nucleolus, where it may regulate chromatin organisation and transcription. In this study, we investigated whether different phosphorylation states of nuclear tau display age- and disease-dependent patterns, with a specific focus on the AT8 epitope (phospho-Ser202/Thr205). Methods: We analysed nuclear tau epitopes (Tau-1, AT8, PHF1, T181, and S262) by indirect immunofluorescence in SK-N-BE neuroblastoma cells under proliferative and retinoic acid-induced differentiated conditions and in post-mortem hippocampal CA1 neurons from foetal, young, aged, and AD brains. Other functional markers (UBTF, Ki67, fibrillarin and acetylated histone H4) were used to assess nuclear organisation and function. Results: Compared with the other epitopes, AT8 was unique in showing dynamic nuclear localisation: absent in proliferating cells but present after differentiation, abundant in young neurons, and significantly reduced in aged and AD samples. Nuclear AT8 co-localised with Ki67, and its decline was associated with neuronal cell cycle re-entry and nucleolar disorganisation. Conclusions: Among multiple nuclear tau epitopes, AT8 was the only one displaying age- and disease-related changes, and its reduction during ageing and AD correlates with nuclear stress, aberrant cell cycle activity, and neuronal vulnerability. Loss of nuclear AT8 may therefore represent an early marker of dysfunction in ageing and AD brains. Full article

Background: EHEC O157:H7 causes severe gastrointestinal illness by first colonizing the large intestine. It intimately attaches to the epithelial lining, orchestrating distinctive “attaching and effacing” lesions that disrupt the host’s cellular landscape. While much is known about the well-established virulence factors, there are much to learn about the surface proteins’ roles in a living host. Methods: This study presents the first in vivo characterisation of the surface proteome, i.e., proteosurfaceome, of Escherichia coli O157:H7 EDL933 during intestinal infection, revealing spatial and temporal adaptations critical for colonisation and survival. Using a murine ileal loop model, surface proteomic profiles were analysed at early (3 h) and late (10 h) infection stages across the ileum and colon. Results: In total, 272 proteins were identified, with only 13 shared across all conditions, reflecting substantial niche-specific adaptations. Gene ontology enrichment analyses highlighted dominant roles in metabolic, cellular, and binding functions, while subcellular localisation prediction uncovered cytoplasmic moonlighting proteins with surface activity. Comparative analyses revealed dynamic changes in protein abundance. Conclusions: These findings indicate a coordinated shift from stress adaptation and virulence to nutrient acquisition and persistence and provide a comprehensive view of EHEC O157:H7 surface proteome dynamics during infection, highlighting key adaptive proteins that may serve as targets for future therapeutic and vaccine strategies. Full article

Overall sitting comfort is related to both static pressure distribution and dynamic human–seat interaction during vibration. This study proposes a simplified finite-element model of the seated human body that could potentially be used to assess overall sitting comfort. The static pressures of the seated human body measured on a rigid seat with different footrest layouts, together with the overall and localised apparent masses of the human body measured in a previous study, were used for model validation. The proposed model contained homogeneous soft tissues of the buttocks and thighs and rigid bodies connected to represent the torso. The tissue geometry was adjusted to match the measured anthropometry. Viscoelastic material was assigned to the tissues, and the properties were identified by fitting the modelled pressures and apparent masses to the measurement results. The proposed model was capable of reproducing static pressures and dynamic forces over the seat for the three sitting postures. Full article

One of the greatest quests in physics in current times is the search for a grand unified theory—to bring all the forces of nature into one coherent explanatory framework. Despite two centuries of progress, both in comprehending the individual forces and formulating mathematical constructs to explain the existence and operation of such forces, the final step to unify the localised atomic and subatomic forces with gravity has proven to be elusive. Whilst recognising that there are arguments for and against the unification of all the forces of nature, the pursuit for unity has been driving many physicists and mathematicians to explore increasingly extraordinary ideas, from string theory to various other options requiring multiple dimensions. Can process philosophy ride to the rescue? By changing our perspective, it might be possible to derive a provocative and compelling alternative way to understand basic (and advanced) physics. This process approach would see all matter objects, at whatever scale, as energetic systems (inherently dynamic). Through the use of game theory, there is a way to appreciate the combination of entropy together with all the apparent forces of nature, being gravity and the more localised forces, within a singular, metaphysically consistent, construct. The outcome, however, challenges our whole understanding of the universe and fundamentally changes our relationship with matter. Full article

Meso-scale combustors face persistent challenges in sustaining stable combustion and efficient heat transfer due to high surface-to-volume ratios and attendant heat losses. In contrast, larger outlet diameters exhibit weaker recirculation and more diffused temperature zones, resulting in reduced combustion efficiency and thermal confinement. The behavior of non-premixed flames in meso-scale combustor has been investigated through a comprehensive numerical study, utilizing computational fluid dynamics (CFD) under stoichiometric natural gas (methane)–air conditions; three outlet configurations (6 mm, 8 mm, and 10 mm) were analysed to evaluate their impact on temperature behaviour, vortex flow, swirl intensity, and central recirculation zone (CRZ) formation. Among the tested geometries, the 6 mm outlet produced the most robust central recirculation, intensifying reactant entrainment and mixing and yielding a sharply localised high-temperature core approaching 1880 K. The study highlights the critical role of geometric parameters in governing heat release distribution, with the 6 mm configuration achieving the highest exhaust temperature (920 K) and peak wall temperature (1020 K), making it particularly suitable for thermoelectric generator (TEG) integration. These findings underscore the interplay between combustor geometry, flow dynamics, and heat transfer mechanisms in meso-scale systems, providing valuable insights for optimizing portable power generation devices. Full article

Prolonged operation of biomedical devices may compromise electronic component integrity due to cyclic thermal stress, thereby impacting both functionality and safety. Regulatory standards require regular inspections, particularly for surgical applications, highlighting the need for efficient and non-invasive diagnostic tools. This study introduces an integrated system that combines finite element models, infrared thermographic analysis, and artificial intelligence to monitor thermal stress in printed circuit boards (PCBs) within biomedical devices. A dynamic thermal model, implemented in COMSOL Multiphysics^® (version 6.2), identifies regions at high risk of thermal overload. The infrared measurements acquired through a FLIR P660 thermal camera provided experimental validation and a dataset for training a hybrid artificial intelligence system. This model integrates deep learning-based U-Net architecture for thermal anomaly segmentation with machine learning classification of heat diffusion patterns. By combining simulation, the proposed system achieved an F1-score of 0.970 for hotspot segmentation using a U-Net architecture and an F1-score of 0.933 for the classification of heat propagation modes via a Multi-Layer Perceptron. This study contributes to the development of intelligent diagnostic tools for biomedical electronics by integrating physics-based simulation and AI-driven thermographic analysis, supporting automatic classification and localisation of thermal anomalies, real-time fault detection and predictive maintenance strategies. Full article

Robust maritime radar object detection and tracking in maritime clutter environments is critical for maritime safety and security. Conventional Constant False Alarm Rate (CFAR) detectors have limited performance in processing complex-valued radar echoes, especially in complex scenarios where phase information is critical and in the real-time processing of successive echo pulses, while existing deep learning methods usually lack native support for complex-valued data and have inherent shortcomings in real-time compared to conventional methods. To overcome these limitations, we propose a dual-branch sequence feature fusion (DFF) detector designed specifically for complex-valued continuous sea-clutter signals, drawing on commonly used methods in video pattern recognition. The DFF employs dual parallel complex-valued U-Net branches to extract multilevel spatiotemporal features from distance profiles and Doppler features from distance–Doppler spectrograms, preserving the critical phase–amplitude relationship. Subsequently, the sequential feature-extraction module (SFEM) captures the temporal dependence in both feature streams. Next, the Adaptive Weight Learning (AWL) module dynamically fuses these multimodal features by learning modality-specific weights. Finally, the detection module generates the object localisation output. Extensive evaluations on the IPIX and SDRDSP datasets show that DFF performs well. On SDRDSP, DFF achieves 98.76% accuracy and 68.75% in F1 score, which significantly outperforms traditional CFAR methods and state-of-the-art deep learning models in terms of detection accuracy and false alarm rate (FAR). These results validate the effectiveness of DFF for reliable maritime object detection in complex clutter environments through multimodal feature fusion and sequence-dependent modelling. Full article

In rotating machines, any faults in anti-friction bearings occurring during operation can lead to failures that are unacceptable due to considerable downtime losses and maintenance costs. Hence, early fault detection is essential, and different vibration-based methods (VBMs) are explored to recognise incipient fault signatures. Based on rotordynamics, if a bearing defect causes metal-to-metal (MtM) impacts during shaft rotation, the impacts excite high-frequency resonance responses of the bearing assembly. The defect-related frequencies are modulated with the resonance responses and rely on signal demodulation for fault detection. However, the current study highlights that the bearing fault/faults may not be detected if the defect in a bearing is not causing MtM impacts nor exciting the high-frequency resonance of the bearing assembly. In a roller bearing, a localised defect may maintain persistent contact between rolling elements and raceways, thereby preventing the occurrence of impulse vibration responses. Due to contact persistence, such defects may not generate impact and may not be detected by existing VBMs, and the bearing could behave as healthy. This paper investigates such specific cases by exploring the relationship between roller-bearing defect characteristics and their potential to generate impact loads during operation. Using an experimental bearing rig, different roller and inner-race defects are presented while their fault characteristic frequencies remain undetected by the envelope analysis, fast Kurtogram, cyclic spectral coherence, and tensor decomposition methods. This study highlights the significance of both the dimension and location of defects within bearings on their detectability based on the rotordynamics concept. Further, simple roller-beam experiments are carried out to visualise and validate the reliability of the experimental observations made on the roller bearing dynamics. Full article

Electricity markets in Europe have undergone major changes in the last decade, mainly due to the increasing share of variable renewable energy sources (RES), changing demand patterns, and geopolitical factors—particularly the war in Ukraine, tensions over energy imports, and disruptions in natural gas supplies. These changes have led to increased electricity price volatility, reducing the reliability of traditional forecasting tools. This research analyses the potential of static and adaptive linear regression as electricity price forecasting tools in the context of three countries with different energy mixes: Germany, France and the Czech Republic. The static regression approach was compared with an adaptive approach based on incremental model updates at monthly intervals. Testing was carried out in three different scenarios combining stable and turbulent market periods. The quantitative results showed that the adaptive model achieved a lower MAE and RMSE, especially when trained on data from high-volatility periods. However, models trained under turbulent conditions performed poorly in stable environments due to a shift in market dynamics. The results supported several of the hypotheses formulated and demonstrated the need for localised, flexible and continuously updated forecasting. Limitations of the adaptive approach and suggestions for future research, including changing the length of training windows and the use of seasonal models, are also discussed. The research confirms that modern markets require adaptive analytical approaches that account for changing RES dynamics and country specificities. Full article

Riverbed dynamics are natural processes that are strongly driven by human and climatic factors. In the last two centuries, the anthropogenic influence and impact of climate change on European rivers has resulted in significant degradation of riverbeds. This research paper aims to determine the historical evolution (1856–2021) and future trends of the Ialomița riverbed (Romania) under the influence of anthropogenic impact and climate change. The case study is a reach of 66 km between the confluences with the Ialomicioara and Pâscov rivers. The localisation in a contact zone between the Curvature Subcarpathians and the Târgoviște Plain, the active recent tectonic uplift of the area, and the intense anthropogenic intervention gives to this river reach favourable conditions for pronounced riverbed dynamics over time. To achieve the aim of the study, we developed a complex methodology which involves the use of Geographical Information System (GIS) techniques, hierarchical cluster analysis (HCA), the Mann–Kendall test (MK), and R programming. The results indicate that the evolution of the Ialomița River aligns with the general trends observed across Europe and within Romania, characterised by a reduction in riverbed geomorphological complexity and a general transition from a braided, multi-thread into a sinuous, single-thread fluvial style. The main processes consist of channel narrowing and incision alternating with intense meandering. However, specific temporal and spatial evolution patterns were identified, mainly influenced by the increasingly anthropogenic local influences and confirmed climate changes in the study area since the second half of the 20th century. Future evolutionary trends suggest that, in the absence of river restoration interventions, the Ialomița riverbed is expected to continue degrading on a short-term horizon, following both climatic and anthropogenic signals. The findings of this study may contribute to a better understanding of recent river behaviours and serve as a valuable tool for the management of the Ialomița River. Full article

With the widespread adoption of the Robot Operating System (ROS), technologies for autonomous navigation in mobile robots have advanced considerably. ROS provides a modular navigation stack that integrates essential components, such as SLAM, localisation, global path planning, and obstacle avoidance, forming the foundation for applications including service robotics and autonomous driving. Nonetheless, achieving safe and reliable navigation in complex and dynamic environments remains a formidable challenge, due to the need for real-time perception of moving obstacles, sensor fusion requirements, and the demand for robust and efficient algorithms. This study presents a systematic examination of the ROS-based navigation stack and obstacle-avoidance mechanisms. The architecture and implementation principles of the core modules are analysed, along with a comparison of the features and application suitability of common local planners such as the Dynamic Window Approach (DWA) and Timed Elastic Band (TEB). The key technical challenges in autonomous navigation are summarised, and recent advancements are reviewed to outline emerging trends in ROS-based systems, including integration with deep learning, multi-robot coordination, and real-time optimisation. The findings contribute to a deeper theoretical understanding of robotic navigation and offer practical guidance for the design and development of autonomous systems. Full article

Wind erosion poses a major threat to ecosystem stability and land productivity in arid and semi-arid regions. Accurate identification of its spatiotemporal dynamics and underlying driving mechanisms is a critical prerequisite for effective risk forecasting and targeted erosion control. This study applied the Revised Wind Erosion Equation (RWEQ) model to assess the spatial distribution, interannual variation, and seasonal dynamics of the Soil Wind Erosion Modulus (SWEM) across Inner Mongolia from 1990 to 2022. The GeoDetector model was further employed to quantify dominant drivers, key interactions, and high-risk zones via factor, interaction, and risk detection. The results showed that the average SWEM across the study period was 35.65 t·ha⁻¹·yr⁻¹ and showed a decreasing trend over time. However, localised increases were observed in the Horqin and Hulun Buir sandy lands and central grasslands. Wind erosion was most intense in spring (17.64 t·ha⁻¹·yr⁻¹) and weakest in summer (5.57 t·ha⁻¹·yr⁻¹). Gale days, NDVI, precipitation, and wind speed were identified as dominant drivers. Interaction detection revealed non-linear synergies between gale days and temperature (q = 0.40) and wind speed and temperature (q = 0.36), alongside a two-factor interaction between NDVI and precipitation (q = 0.19). Risk detection indicated that areas with gale days > 58, wind speed > 3.01 m/s, NDVI < 0.2, precipitation of 30.17–135.59 mm, and temperatures of 3.01–4.23 °C are highly erosion-prone. Management should prioritise these sensitive and intensifying areas by implementing site-specific strategies to enhance ecosystem resilience. Full article

The structural integrity of cement concrete pavements, paramount for ensuring traffic safety and operational efficiency, faces mounting challenges from the escalating burden of heavy-duty vehicular traffic. Precise characterisation of pavement dynamic responses under such conditions proves indispensable for implementing effective structural health monitoring and early warning system deployment. This investigation examines the triaxial dynamic response characteristics of cement concrete pavement subjected to low-speed, heavy-duty vehicular excitations, employing data acquired through in situ field measurements. A monitoring system incorporating embedded triaxial MEMS accelerometers was developed to capture vibration signals directly within the pavement structure. Raw data underwent preprocessing utilising a smoothing wavelet transform technique to attenuate noise, followed by empirical mode decomposition (EMD) and short-time energy (STE) analysis to scrutinise the time–frequency and energetic properties of triaxial vibration signals. The findings demonstrate that heavy, slow-moving vehicles generate substantial triaxial vibrations, with the vertical (Z-axis) response exhibiting the greatest amplitude and encompassing higher dominant frequency components compared to the horizontal (X and Y) axes. EMD successfully decomposed the complex signals into discrete intrinsic mode functions (IMFs), identifying high-frequency components (IMF1–IMF3) associated with transient vehicular impacts, mid-frequency components (IMF4–IMF6) presumably linked to structural and vehicle dynamics, and low-frequency components (IMF7–IMF9) representing system trends or ambient noise. The STE analysis of the selected IMFs elucidated the transient nature of axle loading, revealing pronounced, localised energy peaks. These findings furnish a comprehensive understanding of the dynamic behaviour of cement concrete pavements under heavy vehicle loads and establish a robust methodological framework for pavement performance assessment and refined axle load identification. Full article

Traditional park designs no longer meet the diverse needs of young users amid rising visitor numbers and environmental challenges. Exploring the impact of mountain city parks on youth is crucial, yet localised studies on their spatial perceptions in such unique environments are lacking. Landscape design based on spatial perception evaluation offers a promising approach for renewing mountain parks to address these complex needs. Therefore, a pilot study was conducted in Chongqing’s Pipa Mountain and Eling Parks, involving questionnaire surveys and on-site spatial data collection. Using principal component analysis to select the visual and auditory indicators most related to environmental satisfaction in the overall park and various types of gathering spaces, the results showed that the first principal component of the visual environment in the entrance platform and key nodes (r = 0.41, r = 0.45), as well as the first principal component of the auditory environment in the entrance platform, path platform, and elevated points (r = 0.67, r = 0.85, r = 0.68), all showed significant positive correlations with environmental satisfaction (p < 0.01). Moreover, naturalness and aesthetics were identified as the main factors influencing environmental satisfaction. A random forest model analysed nonlinear relationships, ranking spatial factors by importance. Simultaneously, SHAP analysis highlighted the effects of key factors like elevation changes, green view index, colour diversity, and natural elements. Elevation changes were positively correlated with satisfaction at elevated points but showed a negative correlation in the overall park environment and other gathering spaces. This study explored space-perception dynamics in mountain city parks, proposing strategies to improve environmental quality in various gathering spaces and the park. These findings support creating liveable mountainous environments and guide “human-centred health,” quality enhancement, and sustainable development in renewing mountain city parks. Full article

The ability of a virus to be propagated within a host cell is dependent on a multitude of dynamic virus–host interactions. Live-cell imaging is an invaluable approach in the study of virus replication cycles and virus–host interactions as it can allow for the direct visualisation of key events and interactions in real time. These details can provide unique insights into many aspects of viral infections including the cellular pathways that are exploited by viruses, the evasion of host immune defences, and viral pathogenesis. This review summarises the live-cell fluorescence imaging approaches that have been developed and applied to study Flaviviridae virus family members that are responsible for significant public health burdens and outbreaks which, in many instances, are increasing in frequency and severity. We discuss how these approaches have expanded our understanding of fundamental stages of viral replication cycles by enabling the direct visualisation of the localisation, trafficking, and interactions of virus particles, proteins, and genomes at distinct stages. The strategies that can be employed to enhance the biological relevance of live-cell fluorescence imaging acquisitions are discussed, along with how live-cell imaging approaches can be further developed to increase resolution, enable multi-colour imaging, and support the long-term visualisation of multiple stages of a viral replication cycle. Full article