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Volume 13
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Article

Temporal and Spatial Profiling of Escherichia coli O157:H7 Surface Proteome: Insights into Intestinal Colonisation Dynamics In Vivo

by
Ricardo Monteiro
1,2,3,*,
Ingrid Chafsey
2,
Charlotte Cordonnier
4,
Valentin Ageorges
2,
Didier Viala
5,
Michel Hébraud
2,5,
Valérie Livrelli
4,
Alfredo Pezzicoli
3,
Mariagrazia Pizza
6 and
Mickaël Desvaux
2,*,†
1
Instituto de Investigação e Inovação em Saúde-i3S, Universidade do Porto, 4150-564 Porto, Portugal
2
INRAE, Université Clermont Auvergne, UMR0454 MEDIS, 63000 Clermont-Ferrand, France
3
GSK, 53100 Siena, Italy
4
M2iSH, UMR 1071 INSERM, University of Clermont Auvergne, 63001 Clermont-Ferrand, France
5
INRAE, Plateforme d’Exploration du Métabolisme—Composante Protéomique (PFEMcp), 63122 Saint-Genès-Champanelle, France
6
Centre for Bacterial Resistance Biology, Department of Life Sciences, Imperial College, London SW7 2AZ, UK
*
Authors to whom correspondence should be addressed.
†
Current address: INRAE (Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement), Centre de Recherche Clermont Auvergne-Rhône-Alpes, UMR454 MEDIS (Microbiologie, Environnement Digestif, Santé), Site de Theix, F-63122 Saint-Genès Champanelle, France.
Proteomes 2025, 13(4), 52; https://doi.org/10.3390/proteomes13040052
Submission received: 11 July 2025 / Revised: 30 September 2025 / Accepted: 7 October 2025 / Published: 10 October 2025
Versions Notes

Abstract

Background: EHEC O157:H7 causes severe gastrointestinal illness by first colonizing the large intestine. It intimately attaches to the epithelial lining, orchestrating distinctive “attaching and effacing” lesions that disrupt the host’s cellular landscape. While much is known about the well-established virulence factors, there are much to learn about the surface proteins’ roles in a living host. Methods: This study presents the first in vivo characterisation of the surface proteome, i.e., proteosurfaceome, of Escherichia coli O157:H7 EDL933 during intestinal infection, revealing spatial and temporal adaptations critical for colonisation and survival. Using a murine ileal loop model, surface proteomic profiles were analysed at early (3 h) and late (10 h) infection stages across the ileum and colon. Results: In total, 272 proteins were identified, with only 13 shared across all conditions, reflecting substantial niche-specific adaptations. Gene ontology enrichment analyses highlighted dominant roles in metabolic, cellular, and binding functions, while subcellular localisation prediction uncovered cytoplasmic moonlighting proteins with surface activity. Comparative analyses revealed dynamic changes in protein abundance. Conclusions: These findings indicate a coordinated shift from stress adaptation and virulence to nutrient acquisition and persistence and provide a comprehensive view of EHEC O157:H7 surface proteome dynamics during infection, highlighting key adaptive proteins that may serve as targets for future therapeutic and vaccine strategies.
Keywords: EHEC O157:H7; surface proteome; host colonization EHEC O157:H7; surface proteome; host colonization

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MDPI and ACS Style

Monteiro, R.; Chafsey, I.; Cordonnier, C.; Ageorges, V.; Viala, D.; Hébraud, M.; Livrelli, V.; Pezzicoli, A.; Pizza, M.; Desvaux, M. Temporal and Spatial Profiling of Escherichia coli O157:H7 Surface Proteome: Insights into Intestinal Colonisation Dynamics In Vivo. Proteomes 2025, 13, 52. https://doi.org/10.3390/proteomes13040052

AMA Style

Monteiro R, Chafsey I, Cordonnier C, Ageorges V, Viala D, Hébraud M, Livrelli V, Pezzicoli A, Pizza M, Desvaux M. Temporal and Spatial Profiling of Escherichia coli O157:H7 Surface Proteome: Insights into Intestinal Colonisation Dynamics In Vivo. Proteomes. 2025; 13(4):52. https://doi.org/10.3390/proteomes13040052

Chicago/Turabian Style

Monteiro, Ricardo, Ingrid Chafsey, Charlotte Cordonnier, Valentin Ageorges, Didier Viala, Michel Hébraud, Valérie Livrelli, Alfredo Pezzicoli, Mariagrazia Pizza, and Mickaël Desvaux. 2025. "Temporal and Spatial Profiling of Escherichia coli O157:H7 Surface Proteome: Insights into Intestinal Colonisation Dynamics In Vivo" Proteomes 13, no. 4: 52. https://doi.org/10.3390/proteomes13040052

APA Style

Monteiro, R., Chafsey, I., Cordonnier, C., Ageorges, V., Viala, D., Hébraud, M., Livrelli, V., Pezzicoli, A., Pizza, M., & Desvaux, M. (2025). Temporal and Spatial Profiling of Escherichia coli O157:H7 Surface Proteome: Insights into Intestinal Colonisation Dynamics In Vivo. Proteomes, 13(4), 52. https://doi.org/10.3390/proteomes13040052

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