Search Results (765)

Despite the centrality of scalar implicature (SI) in pragmatic development, the neurocognitive trajectory of SI processing in Mandarin-speaking children remains underexplored, with existing frameworks inadequately accounting for developmental constraints and cross-linguistic variation. This ERP study maps the neurocognitive trajectory of scalar implicature (SI) processing in Mandarin preschoolers (N = 49). Behavioral accuracy improved with age (p < 0.001) but was not modulated by contextual felicity. Neural dynamics revealed developmental shifts: 4-year-olds exhibited heightened P200 amplitudes in infelicitous contexts, indicating attentional overloading. Differences in P200 amplitude between younger and older children indexed developmental shifts in attentional allocation. The N400 showed contextual sensitivity, whereas the Late Positive Component (LPC) showed only marginal context effects, suggesting protracted inferential adjustments. We propose the Cognitive-Dynamic Relevance Model (CDRM), challenging existing frameworks by integrating gradual recalibration mechanisms with resource constraints. Mandarin children demonstrate delayed SI maturation, attributable to reduced SI frequency in child-directed speech and quantifier ambiguity. Findings underscore cross-linguistic variation in pragmatic development, with neurocognitive markers preceding behavioral mastery. Full article

Soybean seed quality is defined by an inverse relationship between oil and protein content. Understanding the spatiotemporal regulation of this trade-off is crucial for breeding. This study aims to dissect the transcriptomic networks governing carbon and nitrogen partitioning during seed development. Here, transcriptomic and co-expression network analyses were performed on cotyledon and seedcoat tissues of high-protein (HP) and low-protein (LP) soybean cultivars across three seed developmental stages. We identified 4910 HP/LP-specific differentially expressed genes (DEGs), with striking transcriptional alterations in the early developmental stage. Notably, some important DEGs were enriched in carbon/lipid metabolism, protein folding, and hormone/circadian signaling pathways, among which key gene families (e.g., OLEs, SWEETs, HSPs), core regulators (e.g., LACS, L1L, ABF1), and QTL-localized candidate genes (e.g., FA9) were characterized. Mechanistically, C/VIF1-mediated post-translational inhibition of CWINV1 may restrict carbon flux to oil synthesis in HP seeds; upstream circadian/hormone signaling and L1L-sHSPs jointly promote protein deposition, uncoupling the oil–protein trade-off and enabling HP trait formation. In contrast, LP cultivars upregulated SWEETs, OLEs, and LTPs to facilitate high carbon flux into lipid biosynthesis and storage. These findings provide valuable genetic targets for precision breeding programs aimed at optimizing resource allocation. Full article

Performance of Osmia bicornis larvae fed on six diets with different pollen species composition (one wild collected by foraging adults), each with known levels of nine essential amino acids (EAA; leucine, lysine, valine, arginine, isoleucine, phenylalanine, threonine, histidine, methionine), was investigated. Four of the pollen diets consisted of individual pollen species and two were mixtures of either four or five species (including one naturally foraged by adult O. bicornis). The diets fell into four statistically distinct groups with different EAA contents (ranked from Group I (highest EAA) to Group IV (lowest EAA; pine pollen). The highest larval survival rate was recorded with the wild-foraged diet (Group III) with no survival in Group IV. Similar survival occurred for all other diets. Where larvae survived (Group I–III), there was no effect of diet on the time to commencement of larval stages, cocoon completion or larval development time (egg hatch to pupation), or on pupal weight. The findings provide corroborative evidence of the existence of amino acid thresholds for larval success, but the need for further work is discussed in relation to their multidimensional nutritional requirements and variation of the nutritional content of pollen. Full article

The article is based on On the Move a holistic, playful movement intervention with children in Red Cross asylum centers in Denmark. Children in asylum centers in Denmark have diverse backgrounds, challenges, and resources. Common challenges due to their life situations can include potential trauma stemming from flight, migration, and/or war experienced by the children and their parents. Furthermore, they live with uncertainty regarding future relocation. These conditions may induce a state of alert, as the children’s foundations feel insecure. These circumstances can also affect the children’s emotional, cognitive, motor, and relational developmental processes. On the Move is a practice-based research project focused on examining how participation in a long-term holistic, playful movement intervention can support children in asylum centers regarding connectedness. The research project is inspired by a phenomenological understanding of body and movement, hermeneutic–phenomenological research, practitioner research, and Arts-Based Research. The data presented here is derived from scenic descriptions and interviews collected during the research project. The theoretical framework is based on the concepts of ontological security, movement philosophy and movement psychology. The article illuminates one of the main practice-based thematic findings from the research project: “Children sway—movement processes”. The article highlights challenges faced by the children due to their life situations and shows how teachers can support the children’s participation in the intervention. The article focuses both on the children’s life situations viewed by professionals and on the children’s movement processes during the intervention. In the movement processes, the children can enter a state in which they are described as being in harmony with the movements, with themselves, and with others. In this way, participating in a holistic, playful movement intervention can support the connectedness of children in asylum centers. Full article

Mechanical energy, a ubiquitous renewable resource, can be effectively harnessed via piezocatalysis to convert physical stimuli into chemical energy for sterilization. As a promising green technology, piezocatalysis employs external mechanical force to physically disrupt bacterial membranes while simultaneously triggering redox reactions to generate bactericidal reactive oxygen species (ROS). Recent advances highlight the superior performance and broad applicability of this technology in the antibacterial domain. This review systematically elucidates the antibacterial mechanisms of piezocatalysis, followed by a comprehensive survey of prevalent piezoelectric biomaterials (e.g., amino acids, cellulose, proteins) and their synthesis strategies. Furthermore, specific applications of these bio-piezoelectric composites in sterilization are consolidated. Finally, we critically assess the primary challenges and outline future developmental trajectories, offering a prospective pathway for next-generation eco-friendly disinfection strategies. Full article

Edible mushrooms have long been valued as functional foods and traditional remedies, yet a significant developmental gap hinders their transition from nutraceuticals to standardized pharmaceutical ingredients. This narrative review provides a comprehensive and integrative analysis of edible mushroom-derived bioactive compounds as emerging candidates for pharmaceutical development. It examines major chemical classes, including polysaccharides (e.g., β-glucans), proteins (e.g., lectins, FIPs), triterpenoids (e.g., ganoderic acids), nucleosides (e.g., adenosine and cordycepin), and phenolic compounds, which underpin immunomodulatory, anticancer, antioxidant, anti-inflammatory, and metabolic activities. Beyond bioactivity, the review critically examines the downstream processing pipeline required for translation into pharmaceutical ingredients, encompassing controlled biomass production, pre-extraction processing, extraction technologies, isolation and purification strategies, and structural elucidation techniques. Key bottlenecks are identified, including bioavailability limitations of β-glucans (2–5%), lack of standardization, limited human clinical evidence, and regulatory constraints, explaining why robust preclinical evidence has not consistently translated into clinical success. Emerging solutions are also highlighted, including application of multi-omics tools, nano-encapsulation strategies, and synthetic biology approaches to improve scalability and reproducibility. By synthesizing research on natural product chemistry, biotechnology, and pharmacology, this study maps the journey of edible mushrooms from traditional dietary components to pharmaceutical-grade ingredients, providing a focused resource for researchers and industry stakeholders aiming to navigate mushroom-based drug development. Full article

Physical and competitive environments play an important role in shaping athletes’ psychological development, motivation, and long-term engagement in sport. Guided by the Personal Assets Framework, this study examined basketball athletes’ perceptions of the quality of facilities and material resources across developmental stages and explored how these environments influenced developmental experiences and continuity in youth sport. A mixed-methods sequential explanatory design was employed. Quantitative data were collected from Brazilian basketball athletes aged 18–19 years (n = 141), followed by semi-structured interviews with 24 athletes. Distributional differences were explored using Kruskal–Wallis tests and associations using chi-square tests, while qualitative data were analysed through thematic analysis. Results indicated that both public and private gyms were commonly used practice settings; however, private facilities were consistently perceived as offering superior structural conditions and material resources, particularly from early adolescence onward. Although physical environments were largely perceived as non-limiting during childhood, their influence on psychological development, motivation, and perceived developmental opportunities became progressively more salient with age. Overall, the findings highlight the importance of adequate physical and competitive environments as key contextual components of athlete development, suggesting that long-term participation in youth sport is strongly influenced by the contexts in which athletes are embedded. Full article

Deciphering the molecular regulatory networks of panicle development is essential for the genetic improvement of crop yield. However, to date, no systematic studies of gene expression across different panicle developmental stages have been performed in foxtail millet. In this study, a transcriptome analysis of foxtail millet panicle was conducted in four stages of development spanning from the initiation of primary branches to floral organ differentiation. A total of 20,377 expressed genes were identified, including 10,647 differentially expressed genes. Fuzzy C-means clustering detected stage-specific expressed homologs of key transcription regulators, which may be involved in plant organ morphogenesis. Moreover, 1215 transcription factors of 55 families were identified. Members of the MADS-box and GRAS transcription factors showed stage-specific expression patterns, whose conservation was useful in discovering genes related to foxtail millet panicle development. Our analysis was further substantiated by the functional characterization of SiSOC1, whose expression exhibited a gradual upregulation across the four developmental stages. SiSOC1 was identified and validated, which revised the annotation of the reference genome. Overexpression of SiSOC1 markedly accelerated the heading of foxtail millet under long-day and short-day conditions, validating its function in regulating the heading date. In summary, this study systematically identified the transcriptomic landscape of foxtail millet panicle development, and elucidated key regulatory genes. These results provide a theoretical foundation and gene resources for facilitating the molecular breeding of foxtail millet. Full article

Early life experiences and the process of exploration play a vital role in shaping brain development and lifelong learning. In March 2020, population-wide restrictions were imposed due to the COVID-19 pandemic. It remains to be determined whether having been raised under the global stress and restrictions of COVID-19 has influenced children’s development as they enter formal schooling. The aim of this study was to examine the extent to which having more than 50% of one’s first year of life and/or prenatal period in the COVID-19 era influences the developmental trajectory in preschool. The study compared 3- to 5-year-old children born before the pandemic (n = 63) with those who were five months or younger at its onset (n = 40). Variables assessed included executive function skills, vocabulary, and common developmental domains. Using the BRIEF-P as a standardized measure of executive function, the results demonstrate that the pandemic-born cohort exhibit greater impairments than those born before the pandemic. There was also a significant increase in reports of speech and language therapy enrollment; frequent ear infections; diagnoses of hearing, speech, or language impairments; and delays in reaching developmental milestones. The pandemic-born cohort additionally reported delays in fine motor skills compared to the pre-pandemic cohort. The present study underscores the urgent need for additional resources to better support children in this cohort as they begin formal schooling. Full article

Background/Objectives: Early Childhood Intervention (ECI) services are critical for supporting children with developmental needs and their families. Despite an established legislative framework, challenges related to accessibility, equity, resources, and standardization of practices persist. This study aimed to describe the perspectives of early intervention professionals in Portugal regarding current barriers, facilitators, and priority areas for improvement within the system. Methods: A descriptive study was conducted involving 82 professionals working in early intervention in Portugal. Data were collected using a survey specifically developed by the research team, grounded in a comprehensive literature review and professional expertise. The instrument was validated through a Delphi Panel with two rounds involving six experts in ECI. Data from open-ended questions were analyzed using content analysis, identifying categories and sub-categories to describe the responses, and descriptive statistics for the closed-ended questions. Results: Professionals highlighted the need to update the National ECI System (SNIPI), improve accessibility, and ensure equitable access to early intervention services. Participants reported limited resources, a lack of standardization in practices, and emphasized the importance of professional training and continuous professional development. The findings also pointed to the urgent need for investment and functional and structural restructuring of early intervention services. Various barriers and facilitators were identified. Conclusions: The study provides valuable insights into the perspectives of early intervention professionals, identifying critical areas for policy improvement, resource allocation, and practice standardization. Full article

Background/Objectives: Adolescence is a critical developmental period in which individuals are required to orient themselves toward the future and construct a coherent life plan, including educational and career aspirations. Future orientation is closely linked to identity development and self-understanding, which allow adolescents to integrate past, present, and anticipated future selves. Among the personal resources supporting this process, problem-solving skills play a key role by enabling effective coping with challenges and informed, goal-directed decision-making. This study examined the association between problem-solving skills and adolescents’ aspirations for an ideal occupation, and tested whether this relationship was mediated by identity acquisition and self-understanding, with attention to gender differences. Methods: A quantitative study design was adopted. Participants were 2443 Italian adolescents (aged 15–19 years) attending upper secondary schools. They completed self-report measures assessing perceived problem-solving skills, identity acquisition, self-understanding, and aspiration for an ideal occupation. Two multigroup mediation models were tested using structural equation modeling, examining identity acquisition and self-understanding as mediators and comparing pathways across genders. Results: Problem-solving skills were indirectly associated with stronger aspirations toward an ideal occupation through identity-related processes. Identity acquisition mediated this association only among females, whereas self-understanding emerged as a significant mediator for both females and males, with partial mediation among females and full mediation among males. Conclusions: Overall, although constrained by the cross-sectional design, the findings are consistent with the notion that problem-solving skills contribute to future-oriented career aspirations chiefly by promoting identity coherence and self-clarity. These findings highlight the importance of integrating problem-solving training with identity-focused interventions in educational and career guidance programs, while considering gender-specific developmental pathways. Full article

Background: Adolescence is a developmental window of heightened vulnerability to psychological distress, yet the interplay between pathology and protective factors remains understudied in a low-to-middle-income urban district in North Lima, Peru. This study examined the network structure of resilience, happiness, and mental health indicators in Peruvian adolescents to identify precise intervention targets. Methods: A sample of 559 adolescents (49.9% boys; M_age = 14.72, SD = 1.43) recruited from public secondary schools in Carabayllo, a low-to-middle-income urban district in North Lima, Peru, completed validated measures of resilience (CD-RISC-25), subjective happiness, and mental health (anxiety, depression, and stress). A Gaussian Graphical Model was estimated using non-regularized partial correlations. Node centrality, predictability, and network stability were assessed, and a Network Comparison Test evaluated structural differences by sex. Results: Anxiety, depression, and stress formed a tightly interconnected core, with the strongest edge between stress and anxiety. Among the psychological resources, self-regulation and external resources showed the highest centrality and predictability, followed by personal competence and tenacity. Happiness occupied a peripheral position but maintained a negative association with depression. The network demonstrated strong stability (CS = 0.75). No significant structural or global strength differences emerged between boys and girls. Conclusions: Findings challenge generic well-being approaches, revealing that happiness is a distal factor rather than a central buffer in this population. Instead, the network architecture suggests that interrupting the stress–anxiety loop and fostering self-regulation skills constitute the most effective pathways for school-based mental health protection, regardless of student gender. Full article

Understanding a species’ life history is essential for assessing its adaptability and resource trade-offs under environmental stress. Given their diversity and ecological significance, Lepidoptera represent an ideal model system for studying such adaptive responses. Under controlled laboratory conditions, we quantified the life history traits of Telchinia issoria and examined their associations with key abiotic factors—temperature, humidity, and light intensity—across all developmental stages. The results showed that: (1) the complete developmental duration from egg to adult was first quantified, establishing a crucial baseline for understanding its life history strategy; (2) the egg stage exhibited the highest survival rate, whereas the eighth-instar larval stage showed the lowest; and (3) correlations with abiotic factors differed markedly across stages, indicating stage-specific environmental sensitivity. Faster larval development may be associated with higher temperature, humidity, and light intensity; pupal development with high humidity and low light; and adult lifespan with low temperature, high humidity, and dim light. These findings advance our understanding of insect developmental plasticity, supporting more accurate population models and informing insect management and biodiversity conservation under climate change. Full article

Computational thinking (CT) has increasingly been recognized as a fundamental skill that should be fostered from early childhood. This study investigated the comparative effectiveness of plugged (robot-based) and unplugged (without technology) instructional activities on the development of CT skills in young children. Two natural classroom groups participated, each receiving the same instructional content and assessment, differing only in intervention modality: one utilized the Bee-bot floor robot, while the other engaged in unplugged activities simulating the robot’s movements. Pre- and post-intervention assessments measured CT and spatial reasoning skills to evaluate learning gains. Results demonstrated significant improvements in CT across both groups, with no statistically significant differences in overall gains, suggesting that unplugged activities, when thoughtfully designed, can be as effective as technology-supported ones. These findings have important implications for designing inclusive and resource-sensitive early childhood CT curricula, emphasizing the value of developmentally appropriate and engaging learning experiences beyond technological availability. Full article

MADS-box transcription factors are key regulators of plant development and environmental responses. Here, we performed an integrated phylogenomic and expression analysis of the MADS-box gene family in Capsicum annuum, identifying 97 members that fall into 52 Type I and 45 Type II genes. Comparative phylogeny, exon–intron organization, conserved motifs, and chromosomal mapping allowed classification into 15 subfamilies. Gene duplication analysis revealed that segmental duplication has been a major driver of family expansion. Expression profiling across multiple tissues, together with promoter cis-element prediction and stress-responsive transcriptome data, demonstrated that Type II genes exhibit broad and dynamic expression patterns, particularly under ABA treatment and temperature stress. A key finding of this study is the complete absence of the Mβ lineage, a Type I subfamily typically associated with gametophyte and endosperm development in other angiosperms. No Mβ-like sequences were detected in the pepper genome, and Type I genes overall showed extremely low expression, suggesting that the Mβ lineage has undergone lineage-specific evolutionary loss and that its functions may be compensated by other Type I members or by expanded Type II regulatory modules. Together, this study provides the first evidence for the evolutionary disappearance of the Mβ subfamily in Capsicum and offers a comprehensive resource for dissecting the developmental and stress-responsive roles of MADS-box genes in pepper. Full article